SMED30012827

Overview
NameSMED30012827
Smed IDSMED30012827
Length (bp)3314
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30012827 (SMED30012827) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30012827 (SMED30012827) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30012827 (SMED30012827) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30012827 (SMED30012827) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012827

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 19

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30012827SMESG000054420.1 SmedASXL_016326SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30012827SMESG000054420.1 dd_Smed_v4_5361_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30012827SMESG000054420.1 dd_Smed_v4_5361_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30012827SMESG000054420.1 dd_Smed_v4_5361_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30012827SMESG000054420.1 dd_Smed_v4_5361_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
parapharyngeal regionSMED30012827SMESG000054420.1 dd_Smed_v4_5361_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30012827SMESG000054420.1 dd_Smed_v4_5361_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30012827SMESG000054420.1 dd_Smed_v4_5361_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012827SMESG000054420.1 Contig45287newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012827SMESG000054420.1 Contig45287uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012827SMESG000054420.1 SMESG000032269.1 Contig30957newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012827SMESG000054420.1 SMESG000032269.1 Contig30957uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012827SMESG000054420.1 SMESG000032269.1 Contig16946newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012827SMESG000054420.1 SMESG000032269.1 Contig16946uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30012827SMESG000054420.1 dd_Smed_v6_5361_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30012827SMESG000054420.1 OX_Smed_1.0.22682ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
neuronSMED30012827SMESG000054420.1 dd_Smed_v6_5361_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cholinergic neuronSMED30012827SMESG000054420.1 dd_Smed_v6_5361_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
GABAergic neuronSMED30012827SMESG000054420.1 dd_Smed_v6_5361_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30012827 vs. Planmine SMEST
Match: SMESG000054420.1 (SMESG000054420.1)

HSP 1 Score: 1553.11 bits (4020), Expect = 0.000e+0
Identity = 874/879 (99.43%), Postives = 874/879 (99.43%), Query Frame = 3 
Query:  273 LSDESISHDSLLNDKYSDCHEEESRLSRKGSYTIELTSEMSQSIRNMRKPLFIKTDMSRHSYASEKSSDEHMRSGNVVTQKNMMTQSMYERPWXXXXXXXXXXXXXQREAKLRLAKEEQSKKLKQKVEGLTEAQKQAEVARKRXXXXXXXXXXXXXXXXXXRQKIVEERKKRLAQAEEARKSRILQKMTNHEEKHSFLQTSIMTMSCYGAVGSSKVRTGNKEVVKDRFTAKRPVSSTGKIIGFGSSSPRSICENSIKTAPKQPPKISDYLMERSQILSRPVSSRMPDRMIISVISPSLSASQNRPMARQNSLKEKRRPVSAMVTSVIDDTIKHKPTRQSKYMSIKENGCENKGVASSTHLRVSSRKSNPTQLTKPNNTQTKPVRDRPSSAKPIQHANNLTERGIIQSKSSTFPKSKDAHQSRRSKVDVHLDSDSIEDEGLTLTESLNQGRSITSSISHKKSEINRTDLSSADSLDTENSKEKTSNNAKSENIKLDKVDGSSSAQLASGRALPTKDLTEADYKARLMXXXXXXXXXXXXXXXQLMKAERERRHLEEATVREIMELEKNKILISEDPQVTTSNDDATEYKSKMKEVQEGATNDKXXXXXXXXXXXXPDQTPENNIXXXXXXXXXXXXXXXXXSIMSRLKKTNXXXXXXXXXVSPRRAMSPELAEIDTETSMATQNQVFRSTLLQSMLNKGKLSGISKTPSPSVKEPEEVKLFQENLFVHSNSNQTSNTVPLTSLRESIEDSESLQINVNSKSSVAVRSSGIDGNSLSDANSDFVVDPETFSNSSVTNNIVLKVDREDCDGQEDHFNENVHSTNCDETNPKEANNSGGPKNYXXXXXXXXXXXXXKNPFNQDHVYNNHIIRPLTQEGFVSQS 2909
            LSDESISHDSLLNDKYSDCHEEESRLSRKGSYTIELTSEMSQSIRNMRKPLFIKTDMSRHSYASEKSSDEHMRSGNVVTQKNMMTQSMYERPWSSSKKNNSISISKQREAKLRLAKEEQSKKLKQKVEGLTEAQKQAEVARKRKENEMIKKLEEKKIKALERQKIVEERKKRLAQAEEARKSRILQKMTNHEEK SFLQTSIMTMSCYGAVGSSKVRTGNKEVVKDRFTAKRPVSSTGKIIGFGSSSPRSICENSIKTAPKQPPKISDYLMERSQILSRPVSSRMPDRMIISVISPSLSASQNRPMARQNSLKEKRRPVSAMVTSVIDDTIKHK TRQSKYMSIKENGCENKGVASSTHLRVSSRKSNPTQLTKPNNTQTKPVRDRPSSAKPIQHANNLTERGIIQSKSSTFPKSKDAHQSRRSKVDVHLDSDSIEDEGLTLTESLNQGRSITSSISHKKSEINRTDLSSADSLDTENSKEKTSNNAKSENIKLDKVDGSSSAQLASGRALPTKDLTEADYKARLMENRRLIREKREREREQLMKAERERRHLEEATVREIMELEKNKILISEDPQVTTSNDDATEYKSKMKEVQEGATNDKQQESSISIEISQPDQTPENNIKLE EEKERLERKKRLESIMSRLKKTNEESSSEGGGVSPRRAMSPELAEIDTETS ATQNQVFRSTLLQSMLNKGKLSGISK PSPSVKEPEEVKLFQENLFVHSNSNQTSNTVPLTSLRESIEDSESLQINVNSKSSVAVRSSGIDGNSLSDANSDFVVDPETFSNSSVTNNIVLKVDREDCDGQEDHFNENVHSTNCDETNPKEANNSGGPKNYSSTSSIETEEEEIKNPFNQDHVYNNHIIRPLTQEGFVSQS
Sbjct:   18 LSDESISHDSLLNDKYSDCHEEESRLSRKGSYTIELTSEMSQSIRNMRKPLFIKTDMSRHSYASEKSSDEHMRSGNVVTQKNMMTQSMYERPWSSSKKNNSISISKQREAKLRLAKEEQSKKLKQKVEGLTEAQKQAEVARKRKENEMIKKLEEKKIKALERQKIVEERKKRLAQAEEARKSRILQKMTNHEEKQSFLQTSIMTMSCYGAVGSSKVRTGNKEVVKDRFTAKRPVSSTGKIIGFGSSSPRSICENSIKTAPKQPPKISDYLMERSQILSRPVSSRMPDRMIISVISPSLSASQNRPMARQNSLKEKRRPVSAMVTSVIDDTIKHKLTRQSKYMSIKENGCENKGVASSTHLRVSSRKSNPTQLTKPNNTQTKPVRDRPSSAKPIQHANNLTERGIIQSKSSTFPKSKDAHQSRRSKVDVHLDSDSIEDEGLTLTESLNQGRSITSSISHKKSEINRTDLSSADSLDTENSKEKTSNNAKSENIKLDKVDGSSSAQLASGRALPTKDLTEADYKARLMENRRLIREKREREREQLMKAERERRHLEEATVREIMELEKNKILISEDPQVTTSNDDATEYKSKMKEVQEGATNDKQQESSISIEISQPDQTPENNIKLENEEKERLERKKRLESIMSRLKKTNEESSSEGGGVSPRRAMSPELAEIDTETSTATQNQVFRSTLLQSMLNKGKLSGISKIPSPSVKEPEEVKLFQENLFVHSNSNQTSNTVPLTSLRESIEDSESLQINVNSKSSVAVRSSGIDGNSLSDANSDFVVDPETFSNSSVTNNIVLKVDREDCDGQEDHFNENVHSTNCDETNPKEANNSGGPKNYSSTSSIETEEEEIKNPFNQDHVYNNHIIRPLTQEGFVSQS 896
[Close]
BLAST of SMED30012827 vs. Planmine SMEST
Match: SMESG000054420.1 (SMESG000054420.1)

HSP 1 Score: 1547.72 bits (4006), Expect = 0.000e+0
Identity = 872/881 (98.98%), Postives = 873/881 (99.09%), Query Frame = 3 
Query:  273 LSDESISHDSLLNDKYSDCHEEESRLSRKGSYTIELTSEMSQSIRNMRKPLFIKTDMSRHSYASEKSSDEHMRSGNVVTQKNMMTQSMYERPWXXXXXXXXXXXX--XQREAKLRLAKEEQSKKLKQKVEGLTEAQKQAEVARKRXXXXXXXXXXXXXXXXXXRQKIVEERKKRLAQAEEARKSRILQKMTNHEEKHSFLQTSIMTMSCYGAVGSSKVRTGNKEVVKDRFTAKRPVSSTGKIIGFGSSSPRSICENSIKTAPKQPPKISDYLMERSQILSRPVSSRMPDRMIISVISPSLSASQNRPMARQNSLKEKRRPVSAMVTSVIDDTIKHKPTRQSKYMSIKENGCENKGVASSTHLRVSSRKSNPTQLTKPNNTQTKPVRDRPSSAKPIQHANNLTERGIIQSKSSTFPKSKDAHQSRRSKVDVHLDSDSIEDEGLTLTESLNQGRSITSSISHKKSEINRTDLSSADSLDTENSKEKTSNNAKSENIKLDKVDGSSSAQLASGRALPTKDLTEADYKARLMXXXXXXXXXXXXXXXQLMKAERERRHLEEATVREIMELEKNKILISEDPQVTTSNDDATEYKSKMKEVQEGATNDKXXXXXXXXXXXXPDQTPENNIXXXXXXXXXXXXXXXXXSIMSRLKKTNXXXXXXXXXVSPRRAMSPELAEIDTETSMATQNQVFRSTLLQSMLNKGKLSGISKTPSPSVKEPEEVKLFQENLFVHSNSNQTSNTVPLTSLRESIEDSESLQINVNSKSSVAVRSSGIDGNSLSDANSDFVVDPETFSNSSVTNNIVLKVDREDCDGQEDHFNENVHSTNCDETNPKEANNSGGPKNYXXXXXXXXXXXXXKNPFNQDHVYNNHIIRPLTQEGFVSQS 2909
            LSDESISHDSLLNDKYSDCHEEESRLSRKGSYTIELTSEMSQSIRNMRKPLFIKTDMSRHSYASEKSSDEHMRSGNVVTQKNMMTQSMYERPWSSSKKNNSIS +  KQREAKLRLAKEEQSKKLKQKVEGLTEAQKQAEVARKRKENEMIKKLEEKKIKALERQKIVEERKKRLAQAEEARKSRILQKMTNHEEK SFLQTSIMTMSCYGAVGSSKVRTGNKEVVKDRFTAKRPVSSTGKIIGFGSSSPRSICENSIKTAPKQPPKISDYLMERSQILSRPVSSRMPDRMIISVISPSLSASQNRPMARQNSLKEKRRPVSAMVTSVIDDTIKHK TRQSKYMSIKENGCENKGVASSTHLRVSSRKSNPTQLTKPNNTQTKPVRDRPSSAKPIQHANNLTERGIIQSKSSTFPKSKDAHQSRRSKVDVHLDSDSIEDEGLTLTESLNQGRSITSSISHKKSEINRTDLSSADSLDTENSKEKTSNNAKSENIKLDKVDGSSSAQLASGRALPTKDLTEADYKARLMENRRLIREKREREREQLMKAERERRHLEEATVREIMELEKNKILISEDPQVTTSNDDATEYKSKMKEVQEGATNDKQQESSISIEISQPDQTPENNIKLE EEKERLERKKRLESIMSRLKKTNEESSSEGGGVSPRRAMSPELAEIDTETS ATQNQVFRSTLLQSMLNKGKLSGISK PSPSVKEPEEVKLFQENLFVHSNSNQTSNTVPLTSLRESIEDSESLQINVNSKSSVAVRSSGIDGNSLSDANSDFVVDPETFSNSSVTNNIVLKVDREDCDGQEDHFNENVHSTNCDETNPKEANNSGGPKNYSSTSSIETEEEEIKNPFNQDHVYNNHIIRPLTQEGFVSQS
Sbjct:   18 LSDESISHDSLLNDKYSDCHEEESRLSRKGSYTIELTSEMSQSIRNMRKPLFIKTDMSRHSYASEKSSDEHMRSGNVVTQKNMMTQSMYERPWSSSKKNNSISSAISKQREAKLRLAKEEQSKKLKQKVEGLTEAQKQAEVARKRKENEMIKKLEEKKIKALERQKIVEERKKRLAQAEEARKSRILQKMTNHEEKQSFLQTSIMTMSCYGAVGSSKVRTGNKEVVKDRFTAKRPVSSTGKIIGFGSSSPRSICENSIKTAPKQPPKISDYLMERSQILSRPVSSRMPDRMIISVISPSLSASQNRPMARQNSLKEKRRPVSAMVTSVIDDTIKHKLTRQSKYMSIKENGCENKGVASSTHLRVSSRKSNPTQLTKPNNTQTKPVRDRPSSAKPIQHANNLTERGIIQSKSSTFPKSKDAHQSRRSKVDVHLDSDSIEDEGLTLTESLNQGRSITSSISHKKSEINRTDLSSADSLDTENSKEKTSNNAKSENIKLDKVDGSSSAQLASGRALPTKDLTEADYKARLMENRRLIREKREREREQLMKAERERRHLEEATVREIMELEKNKILISEDPQVTTSNDDATEYKSKMKEVQEGATNDKQQESSISIEISQPDQTPENNIKLENEEKERLERKKRLESIMSRLKKTNEESSSEGGGVSPRRAMSPELAEIDTETSTATQNQVFRSTLLQSMLNKGKLSGISKIPSPSVKEPEEVKLFQENLFVHSNSNQTSNTVPLTSLRESIEDSESLQINVNSKSSVAVRSSGIDGNSLSDANSDFVVDPETFSNSSVTNNIVLKVDREDCDGQEDHFNENVHSTNCDETNPKEANNSGGPKNYSSTSSIETEEEEIKNPFNQDHVYNNHIIRPLTQEGFVSQS 898
[Close]
BLAST of SMED30012827 vs. Planmine SMEST
Match: SMESG000022393.1 (SMESG000022393.1)

HSP 1 Score: 67.3958 bits (163), Expect = 5.078e-11
Identity = 65/190 (34.21%), Postives = 94/190 (49.47%), Query Frame = 3 
Query:  486 MRSGNVVTQKNMMTQSMYERPWXXXXXXXXXXXXXQREAKLRLAKEEQSKKLK--QKVEGLTEAQKQAEVARKRXXXXXXXXXXXXXXXXXXRQKIVEERKKRLAQAEEARKSRILQKMTNHEEKHSFLQTSIMTMSCYGAVGSSKVRTGNKEVVKDR------FTAKRPVSSTGKIIGFGSSSPRSICE 1031
            + S +++T    MTQSMYE                       L K +Q  KL+   K+  L  ++K ++ A++RK  E+  K  E+K K  E+QK  EER+ R+    EA+K RI++K+            S MT SC        + T N+ V+K R      F  KRP+SSTGK+ GFGSS+ R +CE
Sbjct:   65 LDSDDLLTPDFAMTQSMYEP----------------------LEKRKQQAKLEANSKLLNLLASEKISQAAKERKRKEIKLKAMEQKKKNEEKQKQAEERRLRMLADHEAKKKRIIEKIN---------LPSPMTASC--------INTMNRSVLKQRASMGSGFIEKRPISSTGKVFGFGSSATRDVCE 215
The following BLAST results are available for this feature:
BLAST of SMED30012827 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012827 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012827 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012827 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012827 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012827 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012827 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012827 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012827 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012827 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012827 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012827 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012827 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012827 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000054420.10.000e+099.43SMESG000054420.1[more]
SMESG000054420.10.000e+098.98SMESG000054420.1[more]
SMESG000022393.15.078e-1134.21SMESG000022393.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012827 ID=SMED30012827|Name=SMED30012827|organism=Schmidtea mediterranea sexual|type=transcript|length=3314bp
CATCTACCCATTTTTGTTTCCACTACAATTTTCAATAATAAAATAATTTG
CTATTCGCATAAAATTTCCTCAACAATAGCTATTTTCTTATATCTCATGC
GATTTGATAAAATAATTCAGCATTTCGCATTTTCATTAAAAATTACGACA
ATGTTTTATGTAGGAAGTCATTAGGTTTCTGCAGATTAACACCGAACTTT
TCGTTTATTCTAGATTGGTTTATGGAAGACAACTCAAGAAGTAATGACGA
CCGAAATTTTGACAAGGATTCATTATCTGATGAATCTATATCTCATGATT
CATTGTTGAATGATAAATATAGCGATTGTCACGAAGAAGAATCTCGACTT
TCCCGGAAAGGTAGTTATACCATTGAACTTACATCAGAAATGAGTCAAAG
TATACGAAATATGAGAAAGCCACTTTTTATTAAAACTGACATGTCAAGGC
ATTCATATGCTTCAGAAAAGTCATCAGACGAACACATGAGATCGGGAAAC
GTGGTTACACAGAAAAATATGATGACTCAATCAATGTATGAACGTCCATG
GAGTAGCTCTAAAAAAAATAATTCAATATCGATAAGCAAGCAAAGAGAAG
CTAAACTTCGTTTAGCTAAAGAAGAACAATCAAAAAAGCTAAAACAAAAG
GTTGAAGGTTTAACCGAAGCTCAGAAACAAGCTGAAGTTGCCAGAAAACG
AAAGGAAAATGAAATGATTAAAAAGCTTGAAGAGAAAAAAATAAAAGCCT
TAGAACGACAGAAAATTGTTGAAGAACGGAAAAAACGTTTAGCGCAAGCA
GAAGAAGCTCGAAAGTCTCGAATTTTACAGAAAATGACAAATCATGAAGA
AAAACACAGTTTCCTACAAACATCCATAATGACTATGAGCTGTTATGGAG
CAGTAGGTAGTTCGAAAGTTCGAACGGGAAATAAAGAAGTGGTAAAGGAT
AGATTTACTGCGAAGAGACCAGTTTCTTCAACTGGGAAAATAATTGGATT
CGGTTCCAGTTCCCCTAGAAGTATTTGTGAGAATTCTATCAAAACCGCAC
CAAAGCAACCACCAAAGATTTCAGATTATTTGATGGAAAGATCACAAATT
TTATCTCGCCCCGTATCATCTAGAATGCCTGACAGAATGATAATTTCAGT
AATTTCACCAAGTTTGTCAGCCTCACAAAACCGTCCTATGGCCAGACAGA
ATTCTTTGAAAGAAAAACGAAGACCAGTTTCAGCGATGGTCACATCAGTG
ATTGATGACACAATCAAGCATAAACCAACCAGACAATCAAAATACATGTC
CATCAAAGAAAATGGCTGCGAAAATAAAGGTGTTGCATCATCTACTCATT
TGAGAGTTTCTTCTAGAAAATCAAATCCGACCCAGTTGACTAAACCAAAT
AATACTCAAACAAAACCAGTTCGAGATAGACCTTCATCTGCCAAACCCAT
TCAACATGCAAACAACCTGACAGAAAGAGGAATAATCCAGTCGAAATCCA
GTACATTTCCTAAATCAAAAGATGCTCATCAATCAAGAAGATCTAAAGTA
GACGTGCACCTTGACAGTGATAGTATTGAAGATGAAGGATTAACTCTCAC
AGAATCTTTAAATCAAGGAAGATCAATAACGTCTTCAATTTCACATAAAA
AGAGTGAAATCAACAGAACAGATTTAAGCTCTGCCGATTCATTAGATACC
GAGAATTCAAAAGAAAAAACTTCAAACAATGCAAAATCCGAAAATATAAA
ACTTGATAAAGTAGATGGTTCATCTTCTGCACAGCTTGCTTCAGGAAGAG
CTCTCCCAACGAAAGATCTCACCGAGGCAGATTATAAAGCAAGATTAATG
GAAAATAGAAGACTGATTCGAGAAAAAAGAGAAAGAGAAAGAGAACAATT
AATGAAAGCTGAAAGAGAAAGAAGACATCTTGAAGAAGCGACTGTGAGAG
AAATTATGGAATTGGAGAAGAATAAAATACTGATTTCTGAAGATCCGCAA
GTAACGACTTCAAATGATGATGCCACAGAATACAAGTCTAAAATGAAAGA
GGTTCAAGAAGGAGCTACCAATGACAAGCAACAAGAAAGTTCAATTTCCA
TTGAAATATCGCAGCCTGATCAAACTCCGGAAAATAACATTAAATTGGAA
AAGGAAGAAAAAGAAAGATTAGAAAGAAAGAAACGTCTCGAATCGATAAT
GAGTAGATTAAAGAAGACCAATGAAGAGTCATCAAGTGAAGGCGGTGGAG
TCAGTCCAAGACGAGCTATGAGTCCAGAACTGGCTGAAATCGATACTGAA
ACTTCTATGGCAACACAAAATCAAGTATTCCGTTCAACATTACTCCAGTC
AATGTTAAACAAGGGAAAATTATCCGGAATTTCAAAAACTCCTTCTCCTT
CTGTTAAAGAACCAGAAGAAGTGAAATTGTTTCAAGAAAATTTATTTGTT
CATTCTAACTCTAACCAAACGAGTAACACAGTACCGTTGACATCATTACG
CGAATCAATTGAAGATTCTGAATCACTACAGATAAATGTCAACTCAAAAT
CATCAGTAGCCGTTCGCAGTTCCGGAATAGATGGAAACTCACTATCAGAT
GCCAATTCAGATTTCGTCGTTGATCCAGAAACGTTTTCAAATTCATCTGT
AACCAACAATATTGTATTAAAAGTGGATAGAGAAGATTGTGACGGTCAAG
AAGATCATTTCAACGAAAATGTTCATTCAACCAATTGTGACGAAACAAAT
CCGAAAGAAGCAAACAATTCAGGAGGCCCTAAAAACTATTCATCCACTTC
TTCAATTGAAACAGAAGAGGAAGAGATTAAAAATCCCTTTAACCAAGACC
ACGTCTATAATAATCACATCATCAGACCTTTGACTCAAGAAGGTTTTGTC
TCTCAAAGCTAATAAATTTTCAAGGAACAGTCATAGAAAGAAATCAGAAA
TTGTAAAGGTTTAATGCATTTTATTTAGTTGAGAAGCCATAGACAATATT
TTCATTTGACTATTGATTTTGCACATTATTTGAGACTGAACCTCATCGGT
CTATTTGTTAAAAGAAATTGATCTGACTGCCGATCAGGTCAATTTGCGCT
GTGATTGAAAACGTAATTTTTTTCTGTTAGTTTAATTTTTCGTTTTCTCA
AATCATTCTTTTTATAAACAATAAAAATTAAATTTGCTGGTTACTGGATT
CGATTCTGTCCTTTAAATCTTTTAATTAGTTTTGATAGAAACAATTTTCG
GATCGTTTTCTCAGAGCGAGAAAGTTTCTTTTTAACTTGGTAGTGAAGAC
TTGATCTTTGATAT
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protein sequence of SMED30012827-orf-1

>SMED30012827-orf-1 ID=SMED30012827-orf-1|Name=SMED30012827-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=897bp
MEDNSRSNDDRNFDKDSLSDESISHDSLLNDKYSDCHEEESRLSRKGSYT
IELTSEMSQSIRNMRKPLFIKTDMSRHSYASEKSSDEHMRSGNVVTQKNM
MTQSMYERPWSSSKKNNSISISKQREAKLRLAKEEQSKKLKQKVEGLTEA
QKQAEVARKRKENEMIKKLEEKKIKALERQKIVEERKKRLAQAEEARKSR
ILQKMTNHEEKHSFLQTSIMTMSCYGAVGSSKVRTGNKEVVKDRFTAKRP
VSSTGKIIGFGSSSPRSICENSIKTAPKQPPKISDYLMERSQILSRPVSS
RMPDRMIISVISPSLSASQNRPMARQNSLKEKRRPVSAMVTSVIDDTIKH
KPTRQSKYMSIKENGCENKGVASSTHLRVSSRKSNPTQLTKPNNTQTKPV
RDRPSSAKPIQHANNLTERGIIQSKSSTFPKSKDAHQSRRSKVDVHLDSD
SIEDEGLTLTESLNQGRSITSSISHKKSEINRTDLSSADSLDTENSKEKT
SNNAKSENIKLDKVDGSSSAQLASGRALPTKDLTEADYKARLMENRRLIR
EKREREREQLMKAERERRHLEEATVREIMELEKNKILISEDPQVTTSNDD
ATEYKSKMKEVQEGATNDKQQESSISIEISQPDQTPENNIKLEKEEKERL
ERKKRLESIMSRLKKTNEESSSEGGGVSPRRAMSPELAEIDTETSMATQN
QVFRSTLLQSMLNKGKLSGISKTPSPSVKEPEEVKLFQENLFVHSNSNQT
SNTVPLTSLRESIEDSESLQINVNSKSSVAVRSSGIDGNSLSDANSDFVV
DPETFSNSSVTNNIVLKVDREDCDGQEDHFNENVHSTNCDETNPKEANNS
GGPKNYSSTSSIETEEEEIKNPFNQDHVYNNHIIRPLTQEGFVSQS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
PLANA:0000463cholinergic neuron
PLANA:0000464GABAergic neuron
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR008604MAP7_fam
Vocabulary: cellular component
TermDefinition
GO:0015630microtubule cytoskeleton
Vocabulary: biological process
TermDefinition
GO:0000226microtubule cytoskeleton organization
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 539..573
NoneNo IPR availableCOILSCoilCoilcoord: 653..673
NoneNo IPR availableCOILSCoilCoilcoord: 130..211
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 639..669
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 367..447
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 608..683
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 617..638
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..45
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 432..447
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 832..868
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 836..860
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 491..508
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 367..431
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 474..508
IPR008604Microtubule-associated protein 7 familyPFAMPF05672MAP7coord: 530..670
e-value: 6.2E-8
score: 32.5