SMED30012788
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30012788 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4Note: Hover over icons to view figure legend
Alignments
SMED30012788 aligns in the following genomic locations:
Homology
BLAST of SMED30012788 vs. Planmine SMEST
Match: SMESG000022814.1 (SMESG000022814.1) HSP 1 Score: 259.225 bits (661), Expect = 2.943e-83 Identity = 130/145 (89.66%), Postives = 133/145 (91.72%), Query Frame = 3 Query: 3 MWICNGCNHFNWDINAKICKSCYESRYGIECAINKANENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEIINAFKDGKEEKIWICTKCGLFNGYLDSDKCEICECSRKEENGNKYEQNRDRLGNPAGGEIDENWYCWRRGC 437 MWICN CNHFNWDINAKICKSC ESRY IECAINKANENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEIIN KDG++EK+WICTKCGLFNG LDSDKCEICECSRKEE NKYEQNR RLGNPAGGEIDENW CW R C Sbjct: 362 MWICNECNHFNWDINAKICKSCNESRYEIECAINKANENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEIINVLKDGRKEKMWICTKCGLFNGDLDSDKCEICECSRKEEKENKYEQNRGRLGNPAGGEIDENWDCWSRSC 506 HSP 2 Score: 153.295 bits (386), Expect = 6.623e-44 Identity = 78/108 (72.22%), Postives = 85/108 (78.70%), Query Frame = 3 Query: 114 ENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEIINAFKDGKEEKIWICTKCGLFNGYLDSDKCEICECSRKEENGNKYEQNRDRLGNPAGGEIDENWYCWRRGC 437 ENW C + C K N G+ +YCS CGVD+AANQNRGEIIN KDG++EK+WICTKCG NG LDSDKC ICECSRKEE NK EQNR RLGN AGGEIDENW CW R C Sbjct: 216 ENWDCWRRGCMKKNYGNHQYCSHCGVDKAANQNRGEIINVLKDGRKEKMWICTKCGFHNGDLDSDKCGICECSRKEERENKSEQNRGRLGNSAGGEIDENWDCWSRSC 323 HSP 3 Score: 128.642 bits (322), Expect = 3.536e-35 Identity = 82/159 (51.57%), Postives = 97/159 (61.01%), Query Frame = 3 Query: 3 MWICNGCNHFNWDINAKICKSC-----------YESRYGI--ECAINKANENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEIINAFKDGKEEKIWICTKCGLFNGYLDSDKCEICECSRKEENGNKYEQNRDRLGNPAGGEIDENWYCWRRGCM 440 MWIC C FN D+++ C+ C YE G A + +ENW C C K N G+ +YCS CGVDRAANQNRGEIINAFKDGKEEKIWICTKCGLFNG LDSDKCEICE R E++ YE D A G +++ W C + C+ Sbjct: 447 MWICTKCGLFNGDLDSDKCEICECSRKEEKENKYEQNRGRLGNPAGGEIDENWDCWSRSCRKRNYGNHQYCSHCGVDRAANQNRGEIINAFKDGKEEKIWICTKCGLFNGDLDSDKCEICEEMRYEKDA--YEIITDVEMKRAAGVLND-WKCGKCECV 602 HSP 4 Score: 87.8113 bits (216), Expect = 7.062e-21 Identity = 53/126 (42.06%), Postives = 70/126 (55.56%), Query Frame = 3 Query: 3 MWICNGCNHFNWDINAKICKSCYESRYGIE-------------CAINKANENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEIINAFKDGKEEKIWICTKCGLFNGYLDSDKCEICECSRKE 341 MWIC C N D+++ C C SR A + +ENW C C K N G+ +YCS CGVD+AANQNRGEIIN KDG++E++WIC +C FN +++ C+ C SR E Sbjct: 264 MWICTKCGFHNGDLDSDKCGICECSRKEERENKSEQNRGRLGNSAGGEIDENWDCWSRSCRKRNYGNHQYCSHCGVDKAANQNRGEIINVLKDGRKEEMWICNECNHFNWDINAKICKSCNESRYE 389
BLAST of SMED30012788 vs. Planmine SMEST
Match: SMESG000022780.1 (SMESG000022780.1) HSP 1 Score: 185.267 bits (469), Expect = 1.949e-55 Identity = 106/146 (72.60%), Postives = 113/146 (77.40%), Query Frame = 3 Query: 3 MWICNGCNHFNWDINAKICKSCYESRYGIECAINKANENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEIINAFKDGKEEKIWICTKCGLFNGYLDSDKCEICECSRKEENGNKYEQNRDRLGNPAGGEIDENWYCWRRGCM 440 MWICN CNHFN DINAKICKSC ESRY IE AINKANENWKC QSICGKANVGSDKYCS CGVDRAANQNR EIIN KDGKEEKIWICTKCG FNG L SDKCEICE R E++ YE D A GE+++ W C + C+ Sbjct: 467 MWICNECNHFNRDINAKICKSCNESRYEIESAINKANENWKCLQSICGKANVGSDKYCSHCGVDRAANQNR-EIINVLKDGKEEKIWICTKCGFFNGDLGSDKCEICEEMRYEKDA--YEIITDVEMKRASGELND-WKCGKCECV 608 HSP 2 Score: 164.466 bits (415), Expect = 6.929e-48 Identity = 84/113 (74.34%), Postives = 94/113 (83.19%), Query Frame = 3 Query: 3 MWICNGCNHFNWDINAKICKSCYESRYGIECAINKANENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEIINAFKDGKEEKIWICTKCGLFNGYLDSDKCEICECSRKE 341 MWICNGCNHFN DINAKICKSC ESRY IE AINKANENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGE+I +G++EK+WIC +C FN +++ C+ C SR E Sbjct: 382 MWICNGCNHFNRDINAKICKSCNESRYEIESAINKANENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEVIGVLLNGRKEKMWICNECNHFNRDINAKICKSCNESRYE 494 HSP 3 Score: 157.532 bits (397), Expect = 1.914e-45 Identity = 80/109 (73.39%), Postives = 87/109 (79.82%), Query Frame = 3 Query: 111 NENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEIINAFKDGKEEKIWICTKCGLFNGYLDSDKCEICECSRKEENGNKYEQNRDRLGNPAGGEIDENWYCWRRGC 437 +ENW C C K N G+ +YCS CGVD+AANQNRGEIIN KDG++EK+WICTKCG NG LDSDKCEICECSRKEE NK EQNR RLGNPAGGEIDENW CW R C Sbjct: 235 DENWDCWWRGCMKRNYGNHQYCSHCGVDKAANQNRGEIINVLKDGRKEKMWICTKCGFHNGDLDSDKCEICECSRKEEKENKSEQNRGRLGNPAGGEIDENWDCWSRNC 343 HSP 4 Score: 89.7373 bits (221), Expect = 1.548e-21 Identity = 54/126 (42.86%), Postives = 72/126 (57.14%), Query Frame = 3 Query: 3 MWICNGCNHFNWDINAKICKSCYESRYGIE-------------CAINKANENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEIINAFKDGKEEKIWICTKCGLFNGYLDSDKCEICECSRKE 341 MWIC C N D+++ C+ C SR + A + +ENW C C + N G+ +YCS CGVD+AANQNRGEIIN FKDG++EK+WIC C FN +++ C+ C SR E Sbjct: 284 MWICTKCGFHNGDLDSDKCEICECSRKEEKENKSEQNRGRLGNPAGGEIDENWDCWSRNCRERNYGNHQYCSHCGVDKAANQNRGEIINVFKDGRKEKMWICNGCNHFNRDINAKICKSCNESRYE 409 HSP 5 Score: 51.9878 bits (123), Expect = 2.705e-8 Identity = 34/79 (43.04%), Postives = 41/79 (51.90%), Query Frame = 3 Query: 204 NQNRGEIINAFKDGKEEKIWICTKCGLFNGYLDSDKCEICECSRKEENGNKYEQNRDRLGNPAGGEIDENWYCWRRGCM 440 N+ I K+ + W C CG N +L S++C C R+E N DR NPAGGEIDENW CW RGCM Sbjct: 177 NEQMNNISMKNKNDENSSQWKCEYCGNANEFL-SNRCAGCPNLRRESN--------DRSDNPAGGEIDENWDCWWRGCM 246
BLAST of SMED30012788 vs. Planmine SMEST
Match: SMESG000022815.1 (SMESG000022815.1) HSP 1 Score: 49.2914 bits (116), Expect = 1.910e-7 Identity = 27/81 (33.33%), Postives = 46/81 (56.79%), Query Frame = 3 Query: 120 WKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEIINAFKDGKEEKIWICTKCGLFNGYLDSDKCEICECSRKEENGNKYE 362 W+C + CGK N G D +C +C +DR +++ ++ G+ ++IWIC+KCG N ++DKC+ C + + N E Sbjct: 269 WQCWKVNCGKKNKGEDVFCLQCKLDRNPPDPITKLVKSYAGGEMKEIWICSKCGNENRK-ENDKCDRKNCYQSKLNARSKE 348 HSP 2 Score: 46.9802 bits (110), Expect = 1.336e-6 Identity = 31/97 (31.96%), Postives = 49/97 (50.52%), Query Frame = 3 Query: 3 MWICNGCNHFNWDINAKICKSCYESRYGIECAINKANENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEIIN-AFKDGKEEKIWICTKCGLFN 290 +W+C+ C+ N IN IC +C +S +E + WKC + C + N K+C +C + R A ++ +II +G K WIC KC +N Sbjct: 98 LWMCSWCDLIN-HINTSICSACEKSDKNVERFLM-----WKCWKPHCEQLNNPDCKFCKKCFLQRDAPKSLSDIIERQMGNGSILKFWICEKCWKYN 188 HSP 3 Score: 45.0542 bits (105), Expect = 6.288e-6 Identity = 25/85 (29.41%), Postives = 47/85 (55.29%), Query Frame = 3 Query: 108 ANENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEIINAFKDGKEEKIWICTKCGLFNGYLDSDKCEICECSRKEENGNKYE 362 + +W+C C K N G +K+C +C +DR +++ ++ + + ++IWIC+KCG N ++DKC+ C + + N E Sbjct: 397 SQSSWQCWNIDCEKMNKGVNKFCQQCRLDRNPPDPITKLVKSYAEKEIKEIWICSKCGNENRK-ENDKCDRKNCYQSKLNARSKE 480
BLAST of SMED30012788 vs. Planmine SMEST
Match: SMESG000022822.1 (SMESG000022822.1) HSP 1 Score: 45.4394 bits (106), Expect = 3.849e-6 Identity = 26/81 (32.10%), Postives = 44/81 (54.32%), Query Frame = 3 Query: 120 WKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEIINAFKDGKEEKIWICTKCGLFNGYLDSDKCEICECSRKEENGNKYE 362 W+C + CGK N G D +C +C +DR +++ ++ + ++IWIC KCG N ++DKC+ C + + N E Sbjct: 441 WQCWKVNCGKKNKGEDVFCLQCKLDRNPPDPITKLVKSYAGKEIKEIWICEKCGNENRK-ENDKCDKKNCYQSKLNARSKE 520 HSP 2 Score: 45.0542 bits (105), Expect = 6.388e-6 Identity = 30/97 (30.93%), Postives = 48/97 (49.48%), Query Frame = 3 Query: 3 MWICNGCNHFNWDINAKICKSCYESRYGIECAINKANENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEIIN-AFKDGKEEKIWICTKCGLFN 290 +W+C+ C+ N IN IC +C + +E + WKC + C N K+C +C + R A ++ +II +G+ K WIC KC +N Sbjct: 270 IWMCSWCDLIN-HINTSICSACEKRDKSVERFLM-----WKCWKPHCEHLNNPDCKFCKKCFLQRDAPKSLSDIIERQMGNGRILKFWICEKCWKYN 360 The following BLAST results are available for this feature:
BLAST of SMED30012788 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30012788 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30012788 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30012788 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30012788 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30012788 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30012788 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30012788 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30012788 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30012788 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30012788 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30012788 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30012788 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30012788 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 4
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30012788 ID=SMED30012788|Name=SMED30012788|organism=Schmidtea mediterranea sexual|type=transcript|length=440bpback to top protein sequence of SMED30012788-orf-1 >SMED30012788-orf-1 ID=SMED30012788-orf-1|Name=SMED30012788-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=146bp MWICNGCNHFNWDINAKICKSCYESRYGIECAINKANENWKCQQSICGKAback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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