Usp domain-containing protein

Overview
NameUsp domain-containing protein
Smed IDSMED30012777
Length (bp)869
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Usp domain-containing protein (SMED30012777) t-SNE clustered cells

Violin plots show distribution of expression levels for Usp domain-containing protein (SMED30012777) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Usp domain-containing protein (SMED30012777) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Usp domain-containing protein (SMED30012777) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012777

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30012777h1SMcG0021120 dd_Smed_v4_7254_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30012777h1SMcG0021120 dd_Smed_v4_7254_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30012777h1SMcG0021120 dd_Smed_v4_7254_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30012777h1SMcG0021120 dd_Smed_v4_7254_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
muscle cellSMED30012777h1SMcG0021120 dd_Smed_v4_7254_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30012777h1SMcG0021120 dd_Smed_v4_7254_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30012777h1SMcG0021120 dd_Smed_v6_7254_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A5J4P0C2 (Usp domain-containing protein OS=Paragonimus westermani OX=34504 GN=DEA37_0013912 PE=4 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 1.649e-40
Identity = 83/164 (50.61%), Postives = 110/164 (67.07%), Query Frame = 1 
Query:  139 SVELDENKRIILMPVDFSENSERAFNWYHTEFHRENDILFLIHVVNXXXXXXXXXXXXKIGSNTNEFNEIMQEQLETGKKVGHKYLDRCKEKSIKAKFTVHIGSKPGEHIVRLTKEHKINTIVIGNRAISNFRRTILGSVSEYVIRNANCXXXXXXXEKTKKLK 630
            + EL E  RI+LMPVD SE+SERAF WY     R  D L L+H+V      V+Y  +SK  +   EF+  + + LETGK +  K++ RC+   + A+FT+H+G+KPGEHIVRL +EH  + IV+GNR I   RRT LGSVS+Y++ NAN PVV+VPP KT K K
Sbjct:   21 ATELSEANRIVLMPVDGSEHSERAFTWYLENVLRPGDGLCLVHIVEPMSPGVNYGIASKSPALKEEFSRQINKLLETGKLLRTKFITRCESLEVPARFTIHMGTKPGEHIVRLAQEHGAHMIVVGNRGIGTVRRTFLGSVSDYILHNANVPVVIVPPPKTSKKK 184
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A183A884 (Usp domain-containing protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS3169 PE=4 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 3.093e-37
Identity = 74/161 (45.96%), Postives = 109/161 (67.70%), Query Frame = 1 
Query:  148 LDENKRIILMPVDFSENSERAFNWYHTEFHRENDILFLIHVVNXXXXXXXXXXXXKIGSNTNEFNEIMQEQLETGKKVGHKYLDRCKEKSIKAKFTVHIGSKPGEHIVRLTKEHKINTIVIGNRAISNFRRTILGSVSEYVIRNANCXXXXXXXEKTKKLK 630
            L + +R +LMPVD SE+SERAFNWY     R  D L+L H+V  +   ++Y  +SK  +  ++F + + + +E+G+ +  K+L RC+   + A+FT+H+G+KPGEHIVRL ++  +  IV+GNR I   RRT LGSVS+Y++ NAN PV+VVPP K  K K
Sbjct:   23 LSDAERRVLMPVDGSEHSERAFNWYLENIMRPGDGLYLAHIVEPASPGINYGIASKSPAMKDDFAKHINKLVESGRALRGKFLSRCESLGLAARFTIHVGTKPGEHIVRLAQDQNVQMIVVGNRGIGTVRRTFLGSVSDYILHNANVPVIVVPPTKISKKK 183
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A0R3U4W3 (Usp domain-containing protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS1712 PE=4 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 4.870e-37
Identity = 68/142 (47.89%), Postives = 97/142 (68.31%), Query Frame = 1 
Query:  160 KRIILMPVDFSENSERAFNWYHTEFHRENDILFLIHVVNXXXXXXXXXXXXKIGSNTNEFNEIMQEQLETGKKVGHKYLDRCKEKSIKAKFTVHIGSKPGEHIVRLTKEHKINTIVIGNRAISNFRRTILGSVSEYVIRNAN 585
            KR +L+ VD SE+S RAF WY   F +E D L L+HVV    T ++Y   +K    T +FN+ +QE ++ G+K+G  YL +CK   I+A+F +HIG+KPGEHI  L KE +I++IV+G+R I   RRT+LGSV++YVI +A 
Sbjct:   31 KRRVLLSVDCSESSSRAFEWYSNNFAKEGDGLLLVHVVEPVSTGINYGLVTKPALLTEDFNKHLQELVDEGRKLGKTYLQKCKGTGIQARFILHIGAKPGEHICHLAKELQIDSIVMGSRGIGKIRRTLLGSVTDYVIHHAG 172
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A0X3NS25 (Universal stress protein A-like protein (Fragment) OS=Schistocephalus solidus OX=70667 GN=USPAL PE=4 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 1.112e-36
Identity = 72/177 (40.68%), Postives = 112/177 (63.28%), Query Frame = 1 
Query:   55 LSFKINQ*ATMSEEGSEDVKSSPITIKDSVELDENKRIILMPVDFSENSERAFNWYHTEFHRENDILFLIHVVNXXXXXXXXXXXXKIGSNTNEFNEIMQEQLETGKKVGHKYLDRCKEKSIKAKFTVHIGSKPGEHIVRLTKEHKINTIVIGNRAISNFRRTILGSVSEYVIRNAN 585
            L   ++  A M+  G+  + SSP   ++   L   +R +L+ VD SE+S RAF WY T   RE D L L+H+V  +   V+Y  ++K    T +F++ + E ++ GK++G K+L + K   I+A+F +HIG+KPGEHI RL+KE  I+ IV+G+R +   RRT LGSVS+YV+ +A+
Sbjct:    9 LRLPLSTSAGMATAGASPLNSSPKESENGDYLSTAERKVLLSVDGSEDSARAFEWYTTNLLREKDGLILVHIVEPANAGVNYGLATKPAVLTEDFSKHITELVDKGKELGKKFLHQSKALGIRARFVLHIGAKPGEHICRLSKELAIDIIVMGSRGLGKLRRTFLGSVSDYVLHHAS 185
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A2H1CH20 (Universal stress family protein OS=Fasciola hepatica OX=6192 GN=D915_004269 PE=4 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 1.597e-36
Identity = 68/146 (46.58%), Postives = 98/146 (67.12%), Query Frame = 1 
Query:  148 LDENKRIILMPVDFSENSERAFNWYHTEFHRENDILFLIHVVNXXXXXXXXXXXXKIGSNTNEFNEIMQEQLETGKKVGHKYLDRCKEKSIKAKFTVHIGSKPGEHIVRLTKEHKINTIVIGNRAISNFRRTILGSVSEYVIRNAN 585
            L + +R +LMPVD SE+SERAFNWY     R  D L+L H+V  S   ++Y  +SK  +  ++F + + + +E+G+ +  K+L RC+   + A+FT+H+G+KPGEHIVRL ++     IVIGNR I   RRT LGSVS+YV+ NAN
Sbjct:   23 LIDAERRVLMPVDGSEHSERAFNWYLENIMRSGDGLYLAHIVEPSSPGINYGIASKSPAMKDDFAKHINKLVESGRTLRKKFLSRCESLGLAARFTIHVGTKPGEHIVRLAQDQNAQMIVIGNRGIGTVRRTFLGSVSDYVLHNAN 168
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO45800 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRQ2])

HSP 1 Score: 96.6709 bits (239), Expect = 8.703e-25
Identity = 56/141 (39.72%), Postives = 84/141 (59.57%), Query Frame = 1 
Query:  163 RIILMPVDFSENSERAFNWYHTEFHRENDILFLIHVVNXXXXXXXXXXXXKIGSNTNEFNEIMQEQLETGKKVGHKYLDRCKEKSIKAKFTVHIGSKPGEHIVRLTKEHKINTIVIGNRAISNFRRTILGSVSEYVIRNAN 585
            R IL+P+D S++SE AF WY    H E D L L+HV++S+       S    G   +EF   M + L+  K +  KY  + +  S+KAK  V  G KPGE I + +K+   + I++G+R + + RRTILGSVS+YV+ +A+
Sbjct:   10 RRILLPIDSSKHSEDAFEWYVNNMHHEEDELILVHVLDSAAIQTRVSSH---GLVDDEFKNEMNKGLKEVKALEEKYKTKAETASLKAKIEVR-GGKPGETICQCSKDEHCDLILMGSRGLGSIRRTILGSVSDYVLHHAH 146
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO44746 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUS8])

HSP 1 Score: 87.4261 bits (215), Expect = 2.757e-21
Identity = 48/144 (33.33%), Postives = 78/144 (54.17%), Query Frame = 1 
Query:  160 KRIILMPVDFSENSERAFNWYHTEFHRENDILFLIHVVNXXXXXXXXXXXXKIGSN--TNEFNEIMQEQLETGKKVGHKYLDRCKEKSIKAKFTVHIGSKPGEHIVRLTKEHKINTIVIGNRAISNFRRTILGSVSEYVIRNAN 585
            KR +++PVD S++SERAFNWY    H   D + +IH            S    G     +E+N+ + E +   KK+  +Y  +CKE+ +  +     G +PGE I +L K+     +V+G+R     RRT+LGSVS+Y + +++
Sbjct:    6 KRRVVIPVDGSQHSERAFNWYRQHVHEPGDEVLIIHT---QEQPTIPSSPYAYGGTVLPDEWNKAVDECIVNAKKLIEEYNKKCKEQGMTCRLFKGSG-QPGETICQLAKDLSAKHVVMGSRGCGTIRRTLLGSVSDYCVHHSS 145
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO44805 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUT2])

HSP 1 Score: 87.0409 bits (214), Expect = 5.588e-21
Identity = 51/155 (32.90%), Postives = 86/155 (55.48%), Query Frame = 1 
Query:  163 RIILMPVDFSENSERAFNWYHTEFHRENDILFLIHVVNXXXXXXXXXXXXKIGSNTNEFNEIMQEQLETGKKVGHKYLDRCKEKSIKAKFTVHIGSKPGEHIVRLTKEHKINTIVIGNRAISNFRRTILGSVSEYVIRNANCXXXXXXXEKTKKL 627
            R I++PVD S++SERAF+WY    HR ND +F++H  +      +        +   ++ + M++ ++  K V   Y  +CK+  +K       G  PGE I  + K+   + I++G+R +   RRTI+GSVSE+ + + + P+ +VPPEK   L
Sbjct:   10 RKIVIPVDGSKHSERAFDWYKGALHRGNDEVFVVHAFDPYAAPPTPYPYGF--AFPEDWEQHMKKTVDDAKSVMEYYEKKCKDSKMKCTMLTKPGD-PGETICEIAKDKNADQIIMGSRGLGTVRRTIVGSVSEFCLHHTHIPMSIVPPEKPDGL 161
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO33143 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7ST34])

HSP 1 Score: 85.8853 bits (211), Expect = 1.036e-20
Identity = 46/141 (32.62%), Postives = 79/141 (56.03%), Query Frame = 1 
Query:  163 RIILMPVDFSENSERAFNWYHTEFHRENDILFLIHVVNXXXXXXXXXXXXKIGSNTNEFNEIMQEQLETGKKVGHKYLDRCKEKSIKAKFTVHIGSKPGEHIVRLTKEHKINTIVIGNRAISNFRRTILGSVSEYVIRNAN 585
            R IL+PVD SE+SERAF+WY    H   D + ++H +      + +     + +   E++ +++E  E  + +   Y D CKEK +  +  + +G   G+ I ++ ++   N IV+G R     RRTILGSVS+YV+ +++
Sbjct:    4 RKILIPVDGSEHSERAFDWYAELLHSPGDEVLVVHCIELPPVPLEHQFPF-VFAYYEEWSAMVKETREQHEAMLRSYEDICKEKKLHYEIMMVVGKPAGDVICQVARDVSANLIVLGTRGQGMIRRTILGSVSDYVVHHSH 143
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO42478 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1B3])

HSP 1 Score: 85.5001 bits (210), Expect = 1.685e-20
Identity = 45/144 (31.25%), Postives = 81/144 (56.25%), Query Frame = 1 
Query:  160 KRIILMPVDFSENSERAFNWYHTEFHRENDILFLIHVVNXXXXXXXXXXXXKIGSNTNEFNEIMQEQLETGKKVGHKYLDRCKE-KSIKAKFTVHIGSKPGEHIVRLTKEHKINTIVIGNRAISNFRRTILGSVSEYVIRNANC 588
            + ++L+PVD S+NS RAF+WY   +H+END + ++          +  +S    +   E+  + Q+  +  + +   +  RC   K + +   +  G K GE I+ + K+  ++ I+IG+R +  FRRTILGSVS+YV+ +A+ 
Sbjct:    8 RNVVLIPVDGSKNSIRAFDWYKDHYHQENDKVLIVSAYEIPPMQAAKHASVDFKNQLLEWQILRQKAEDKARSILKVFEQRCLPFKELISYRLLPGGGKAGEVIIGIAKQENVDEIIIGSRGLGKFRRTILGSVSDYVVHHASV 151
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000035960.1 (SMESG000035960.1)

HSP 1 Score: 364.77 bits (935), Expect = 1.238e-128
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 1 
Query:   85 MSEEGSEDVKSSPITIKDSVELDENKRIILMPVDFSENSERAFNWYHTEFHRENDILFLIHVVNXXXXXXXXXXXXKIGSNTNEFNEIMQEQLETGKKVGHKYLDRCKEKSIKAKFTVHIGSKPGEHIVRLTKEHKINTIVIGNRAISNFRRTILGSVSEYVIRNANCXXXXXXXEKTKKLKDRNSIDKQSIDSVSQKNED 687
            MSEEGSEDVKSSPITIKDSVELDENKRIILMPVDFSENSERAFNWYHTEFHRENDILFLIHVVNSSGTSVSYDSSSKIGSNTNEFNEIMQEQLETGKKVGHKYLDRCKEKSIKAKFTVHIGSKPGEHIVRLTKEHKINTIVIGNRAISNFRRTILGSVSEYVIRNANCPVVVVPPEKTKKLKDRNSIDKQSIDSVSQKNED
Sbjct:    1 MSEEGSEDVKSSPITIKDSVELDENKRIILMPVDFSENSERAFNWYHTEFHRENDILFLIHVVNSSGTSVSYDSSSKIGSNTNEFNEIMQEQLETGKKVGHKYLDRCKEKSIKAKFTVHIGSKPGEHIVRLTKEHKINTIVIGNRAISNFRRTILGSVSEYVIRNANCPVVVVPPEKTKKLKDRNSIDKQSIDSVSQKNED 201
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000022085.1 (SMESG000022085.1)

HSP 1 Score: 117.472 bits (293), Expect = 2.164e-32
Identity = 58/154 (37.66%), Postives = 101/154 (65.58%), Query Frame = 1 
Query:  154 ENKRIILMPVDFSENSERAFNWYHTEFHRENDILFLIHVVNXXXXXXXXXXXXKIGSNTNEFNEIMQEQLETGKKVGHKYLDRCKEKSIKAKFTVHIGSKPGEHIVRLTKEHKINTIVIGNRAISNFRRTILGSVSEYVIRNANCXXXXXXXEK 615
            ++ R++L+P+D S+NSERAF WY  + HR  D+  LI++V     +   +S+     + +  ++ MQ+ +E GK++  ++L   K  +++AK  +H+  KPG  +V+  KEH+ + IV+GNR +S   RT LGSVS+Y++++A+ P+V+VP EK
Sbjct:    5 KDTRVVLLPLDGSDNSERAFEWYIGQMHRRGDLAVLINIVEPVYATPPLNSAENSVLSNDSVSQQMQDIIEDGKQLTQRFLHMAKNANVEAKAFIHVDPKPGPALVKCAKEHRADVIVVGNRGVSKLHRTFLGSVSDYILQHAHLPIVIVPKEK 158
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000016283.1 (SMESG000016283.1)

HSP 1 Score: 110.153 bits (274), Expect = 1.196e-29
Identity = 47/143 (32.87%), Postives = 87/143 (60.84%), Query Frame = 1 
Query:  160 KRIILMPVDFSENSERAFNWYHTEFHRENDILFLIHVVNXXXXXXXXXXXXKIGSNTNEFNEIMQEQLETGKKVGHKYLDRCKEKSIKAKFTVHIGSKPGEHIVRLTKEHKINTIVIGNRAISNFRRTILGSVSEYVIRNANC 588
            KR I++P+D SE+S+RAF WY     + ND++  IHVV     + +           ++ + +M++ +  GKK+G +++   K+  ++ K  +H+ +KPG  +V+  K+H+ + I+IGNR +   RRT LGSVS++++ +A+ 
Sbjct:    3 KRSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVGMMEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAHI 145
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000039918.1 (SMESG000039918.1)

HSP 1 Score: 100.138 bits (248), Expect = 1.054e-25
Identity = 47/142 (33.10%), Postives = 82/142 (57.75%), Query Frame = 1 
Query:  163 RIILMPVDFSENSERAFNWYHTEFHRENDILFLIHVVNXXXXXXXXXXXXKIGSNTNEFNEIMQEQLETGKKVGHKYLDRCKEKSIKAKFTVHIGSKPGEHIVRLTKEHKINTIVIGNRAISNFRRTILGSVSEYVIRNANC 588
            R IL+PVD S++S RAF WY     +E D+LFL+H+V     + +   S  I     +    M    +  K++  KY+   ++  I  K  +++ +K G+ I+++  E++++ ++IGNR I   RRTILGS S++++ +AN 
Sbjct:    8 RNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYATSAISISDTIKFKMEDMTSKMDSASKKAKELLQKYIKLAEDDRIHTKGIINVDNKAGDAIIKIASENRVSLVIIGNRGIGTLRRTILGSTSDFILNHANV 149
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000020952.1 (SMESG000020952.1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.783e-25
Identity = 54/146 (36.99%), Postives = 88/146 (60.27%), Query Frame = 1 
Query:  151 DENKRIILMPVDFSENSERAFNWYHTEFHRENDILFLIHVVNXXXXXXXXXXXXKIG-SNTNEFNEIMQEQLETGKKVGHKYLDRCKEKSIKAKFTVHIGSKPGEHIVRLTKEHKINTIVIGNRAISNFRRTILGSVSEYVIRNAN 585
            DE +R+ILMPVD SE++ERA +WY     + ND++ +IH+V    TS +     ++      +    +++QL+ GK +  K+    +EK  +AK  V + SK GE I+++   HK++ I++ NR     RRT LGSVS+YV+ +A+
Sbjct:    7 DEVERVILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVEPMYTSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHAD 152
The following BLAST results are available for this feature:
BLAST of Usp domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5J4P0C21.649e-4050.61Usp domain-containing protein OS=Paragonimus weste... [more]
A0A183A8843.093e-3745.96Usp domain-containing protein OS=Echinostoma capro... [more]
A0A0R3U4W34.870e-3747.89Usp domain-containing protein OS=Mesocestoides cor... [more]
A0A0X3NS251.112e-3640.68Universal stress protein A-like protein (Fragment)... [more]
A0A2H1CH201.597e-3646.58Universal stress family protein OS=Fasciola hepati... [more]
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BLAST of Usp domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO458008.703e-2539.72Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO447462.757e-2133.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO448055.588e-2132.90Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO331431.036e-2032.62Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO424781.685e-2031.25Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Usp domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000035960.11.238e-128100.00SMESG000035960.1[more]
SMESG000022085.12.164e-3237.66SMESG000022085.1[more]
SMESG000016283.11.196e-2932.87SMESG000016283.1[more]
SMESG000039918.11.054e-2533.10SMESG000039918.1[more]
SMESG000020952.11.783e-2536.99SMESG000020952.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012777 ID=SMED30012777|Name=Usp domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=869bp
TCTATATAAATCTTATATGTATTTTAGAAAATCTCTTATTACATGTAACT
AAGACTTTCTTTCAAAATAAATCAATAAGCAACAATGTCAGAAGAAGGCA
GTGAAGATGTTAAATCGAGTCCTATTACAATTAAAGACAGTGTAGAATTA
GATGAGAACAAGAGAATTATTCTTATGCCTGTGGATTTTTCAGAAAACAG
TGAACGAGCATTTAATTGGTATCATACGGAATTTCACCGAGAAAATGATA
TTCTATTTTTAATACATGTGGTTAATTCATCTGGAACTTCCGTTTCTTAT
GATAGTTCATCAAAAATTGGATCCAATACAAATGAATTTAACGAAATAAT
GCAGGAACAACTAGAAACAGGGAAAAAGGTCGGTCACAAATATCTTGATC
GATGTAAAGAAAAATCTATTAAGGCAAAATTTACCGTTCATATCGGCAGT
AAACCTGGAGAACACATAGTAAGGCTTACTAAAGAACACAAAATAAATAC
TATAGTTATCGGCAACCGAGCAATAAGTAATTTCCGAAGAACAATTTTGG
GAAGTGTTAGCGAATATGTTATTCGTAATGCAAATTGTCCAGTTGTAGTT
GTTCCACCGGAAAAAACGAAGAAGCTGAAAGACCGAAACTCAATTGACAA
GCAATCCATTGATTCGGTCAGTCAAAAAAATGAAGATAGCTCTAAGAGTG
GAAAATTTGGTTCATTTCTTAAAGGAATTGGTTCAAAATGATTCCGCGCC
ATGGTGCACTTTTTATTTATTTGAGAAATGTCTAAGAAAATTCGGTTACA
ATTTTTATTTTAGTTTAGATCTTTATATTCGTGGTTTTTTATTCGTATGA
TTTTTCATTAAATATATAA
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protein sequence of SMED30012777-orf-1

>SMED30012777-orf-1 ID=SMED30012777-orf-1|Name=SMED30012777-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=219bp
MSEEGSEDVKSSPITIKDSVELDENKRIILMPVDFSENSERAFNWYHTEF
HRENDILFLIHVVNSSGTSVSYDSSSKIGSNTNEFNEIMQEQLETGKKVG
HKYLDRCKEKSIKAKFTVHIGSKPGEHIVRLTKEHKINTIVIGNRAISNF
RRTILGSVSEYVIRNANCPVVVVPPEKTKKLKDRNSIDKQSIDSVSQKNE
DSSKSGKFGSFLKGIGSK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR006015Universal_stress_UspA
IPR014729Rossmann-like_a/b/a_fold
IPR006016UspA
Vocabulary: biological process
TermDefinition
GO:0006950response to stress
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006015Universal stress protein A familyPRINTSPR01438UNVRSLSTRESScoord: 26..44
score: 33.26
coord: 152..174
score: 45.22
coord: 134..146
score: 26.15
IPR006016UspAPFAMPF00582Uspcoord: 27..174
e-value: 6.1E-26
score: 91.6
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3DG3DSA:3.40.50.620coord: 16..186
e-value: 4.8E-38
score: 133.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 179..218
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 190..206
NoneNo IPR availablePANTHERPTHR46989FAMILY NOT NAMEDcoord: 29..203
NoneNo IPR availableCDDcd00293USP_Likecoord: 29..173
e-value: 8.30909E-28
score: 100.522
NoneNo IPR availableSUPERFAMILYSSF52402Adenine nucleotide alpha hydrolases-likecoord: 29..177