Transformer 2 protein

Overview
NameTransformer 2 protein
Smed IDSMED30012773
Length (bp)1585
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Transformer 2 protein (SMED30012773) t-SNE clustered cells

Violin plots show distribution of expression levels for Transformer 2 protein (SMED30012773) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Transformer 2 protein (SMED30012773) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Transformer 2 protein (SMED30012773) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30012773

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30012773h1SMcG0006461 SmedASXL_012137SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30012773h1SMcG0006461 SmedASXL_012137SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30012773h1SMcG0006461 dd_Smed_v4_2971_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30012773h1SMcG0006461 dd_Smed_v4_2971_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30012773h1SMcG0006461 dd_Smed_v4_2971_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30012773h1SMcG0006461 dd_Smed_v4_2971_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30012773h1SMcG0006461 dd_Smed_v4_2971_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Transformer 2 protein vs. Ensembl Human
Match: TRA2B (transformer 2 beta homolog [Source:HGNC Symbol;Acc:HGNC:10781])

HSP 1 Score: 137.887 bits (346), Expect = 1.686e-37
Identity = 62/113 (54.87%), Postives = 86/113 (76.11%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTH 524
            MS ++    +R NP P+ CLG+F LSL TTE+DL E+F +YG I DVS+V D+ ++RSRGF FVYF+N+DDA+ AKE+ +G  + G++IRVD+S+T R HTPTPG+Y G  T+
Sbjct:    1 MSTRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKERANGMELDGRRIRVDFSITKRPHTPTPGIYMGRPTY 113          
BLAST of Transformer 2 protein vs. Ensembl Human
Match: TRA2A (transformer 2 alpha homolog [Source:HGNC Symbol;Acc:HGNC:16645])

HSP 1 Score: 135.191 bits (339), Expect = 1.362e-36
Identity = 62/113 (54.87%), Postives = 83/113 (73.45%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTH 524
            MS+++     R NP P+ CLG+F LSL TTE+DL E+F RYG +  V++V D+ T RSRGF FVYF+ IDD++ A E+ +G  + G++IRVDYS+T RAHTPTPG+Y G  TH
Sbjct:    1 MSNRRRHTGSRANPDPNTCLGVFGLSLYTTERDLREVFSRYGPLSGVNVVYDQRTGRSRGFAFVYFERIDDSKEAMERANGMELDGRRIRVDYSITKRAHTPTPGIYMGRPTH 113          
BLAST of Transformer 2 protein vs. Ensembl Human
Match: TRA2A (transformer 2 alpha homolog [Source:HGNC Symbol;Acc:HGNC:16645])

HSP 1 Score: 135.191 bits (339), Expect = 1.402e-36
Identity = 62/113 (54.87%), Postives = 83/113 (73.45%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTH 524
            MS+++     R NP P+ CLG+F LSL TTE+DL E+F RYG +  V++V D+ T RSRGF FVYF+ IDD++ A E+ +G  + G++IRVDYS+T RAHTPTPG+Y G  TH
Sbjct:    1 MSNRRRHTGSRANPDPNTCLGVFGLSLYTTERDLREVFSRYGPLSGVNVVYDQRTGRSRGFAFVYFERIDDSKEAMERANGMELDGRRIRVDYSITKRAHTPTPGIYMGRPTH 113          
BLAST of Transformer 2 protein vs. Ensembl Human
Match: TRA2A (transformer 2 alpha homolog [Source:HGNC Symbol;Acc:HGNC:16645])

HSP 1 Score: 135.191 bits (339), Expect = 1.402e-36
Identity = 62/113 (54.87%), Postives = 83/113 (73.45%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTH 524
            MS+++     R NP P+ CLG+F LSL TTE+DL E+F RYG +  V++V D+ T RSRGF FVYF+ IDD++ A E+ +G  + G++IRVDYS+T RAHTPTPG+Y G  TH
Sbjct:    1 MSNRRRHTGSRANPDPNTCLGVFGLSLYTTERDLREVFSRYGPLSGVNVVYDQRTGRSRGFAFVYFERIDDSKEAMERANGMELDGRRIRVDYSITKRAHTPTPGIYMGRPTH 113          
BLAST of Transformer 2 protein vs. Ensembl Human
Match: TRA2B (transformer 2 beta homolog [Source:HGNC Symbol;Acc:HGNC:10781])

HSP 1 Score: 138.272 bits (347), Expect = 2.294e-36
Identity = 62/113 (54.87%), Postives = 86/113 (76.11%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTH 524
            MS ++    +R NP P+ CLG+F LSL TTE+DL E+F +YG I DVS+V D+ ++RSRGF FVYF+N+DDA+ AKE+ +G  + G++IRVD+S+T R HTPTPG+Y G  T+
Sbjct:  101 MSTRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKERANGMELDGRRIRVDFSITKRPHTPTPGIYMGRPTY 213          
BLAST of Transformer 2 protein vs. Ensembl Celegans
Match: rsp-8 (SR Protein (Splicing factor) [Source:UniProtKB/TrEMBL;Acc:G3MU77])

HSP 1 Score: 119.783 bits (299), Expect = 5.520e-30
Identity = 55/98 (56.12%), Postives = 72/98 (73.47%), Query Frame = 3
Query:  213 DRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVY 506
            DR+NP PSKCLG+FNLS  TTEKDL ++F  +GEI    LV D+ +  SRGFGF+YF  I+DA  A++K+  T + G +IRVD+S+T R H+PTPG Y
Sbjct:   68 DRENPQPSKCLGVFNLSSYTTEKDLRDVFGEFGEINKCDLVYDRPSGNSRGFGFIYFNLIEDATAARDKLCNTDLDGHKIRVDFSLTKRGHSPTPGQY 165          
BLAST of Transformer 2 protein vs. Ensembl Celegans
Match: rsp-8 (SR Protein (Splicing factor) [Source:UniProtKB/TrEMBL;Acc:G3MU77])

HSP 1 Score: 119.783 bits (299), Expect = 5.520e-30
Identity = 55/98 (56.12%), Postives = 72/98 (73.47%), Query Frame = 3
Query:  213 DRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVY 506
            DR+NP PSKCLG+FNLS  TTEKDL ++F  +GEI    LV D+ +  SRGFGF+YF  I+DA  A++K+  T + G +IRVD+S+T R H+PTPG Y
Sbjct:   68 DRENPQPSKCLGVFNLSSYTTEKDLRDVFGEFGEINKCDLVYDRPSGNSRGFGFIYFNLIEDATAARDKLCNTDLDGHKIRVDFSLTKRGHSPTPGQY 165          
BLAST of Transformer 2 protein vs. Ensembl Celegans
Match: rsp-4 (Probable splicing factor, arginine/serine-rich 4 [Source:UniProtKB/Swiss-Prot;Acc:Q09511])

HSP 1 Score: 66.6254 bits (161), Expect = 3.450e-13
Identity = 34/73 (46.58%), Postives = 47/73 (64.38%), Query Frame = 3
Query:  240 CLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRV 458
             L I NLS +TT  DL   F RYG+I DV +  DK +++S+GFGFV F    DAE A ++  G +V G+++RV
Sbjct:   20 SLKIDNLSYQTTPNDLRRTFERYGDIGDVHIPRDKYSRQSKGFGFVRFYERRDAEHALDRTDGKLVDGRELRV 92          
BLAST of Transformer 2 protein vs. Ensembl Celegans
Match: rsp-4 (Probable splicing factor, arginine/serine-rich 4 [Source:UniProtKB/Swiss-Prot;Acc:Q09511])

HSP 1 Score: 67.0106 bits (162), Expect = 1.249e-12
Identity = 34/73 (46.58%), Postives = 47/73 (64.38%), Query Frame = 3
Query:  240 CLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRV 458
             L I NLS +TT  DL   F RYG+I DV +  DK +++S+GFGFV F    DAE A ++  G +V G+++RV
Sbjct:   20 SLKIDNLSYQTTPNDLRRTFERYGDIGDVHIPRDKYSRQSKGFGFVRFYERRDAEHALDRTDGKLVDGRELRV 92          
BLAST of Transformer 2 protein vs. Ensembl Celegans
Match: fox-1 (Sex determination protein fox-1 [Source:UniProtKB/Swiss-Prot;Acc:Q10572])

HSP 1 Score: 59.6918 bits (143), Expect = 2.054e-9
Identity = 33/105 (31.43%), Postives = 60/105 (57.14%), Query Frame = 3
Query:  204 DKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHT----PTPGVY 506
            D      P   K L + N+     + DL  MF ++G + DV ++ ++  + S+GFGFV  +   DAE A++++HG++++G++I V+ + T R H+    PT G+ 
Sbjct:   83 DPSTSSGPDGPKRLHVSNIPFRFRDPDLKTMFEKFGVVSDVEIIFNE--RGSKGFGFVTMERPQDAERARQELHGSMIEGRKIEVNCA-TARVHSKKVKPTGGIL 184          
BLAST of Transformer 2 protein vs. Ensembl Fly
Match: tra2 (gene:FBgn0003742 transcript:FBtr0089616)

HSP 1 Score: 117.087 bits (292), Expect = 2.635e-30
Identity = 50/103 (48.54%), Postives = 74/103 (71.84%), Query Frame = 3
Query:  210 EDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTK 518
            + R++P  S+C+G+F L+  T++  + E+F +YG IE + +VID  T+RSRGF F+YF+ + DA  AK+   G  V G++IRVD+S+T RAHTPTPGVY G +
Sbjct:    3 KSREHPQASRCIGVFGLNTNTSQHKVRELFNKYGPIERIQMVIDAQTQRSRGFCFIYFEKLSDARAAKDSCSGIEVDGRRIRVDFSITQRAHTPTPGVYLGRQ 105          
BLAST of Transformer 2 protein vs. Ensembl Fly
Match: tra2 (gene:FBgn0003742 transcript:FBtr0089615)

HSP 1 Score: 117.857 bits (294), Expect = 5.914e-30
Identity = 51/109 (46.79%), Postives = 77/109 (70.64%), Query Frame = 3
Query:  192 DKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTK 518
            D++   + R++P  S+C+G+F L+  T++  + E+F +YG IE + +VID  T+RSRGF F+YF+ + DA  AK+   G  V G++IRVD+S+T RAHTPTPGVY G +
Sbjct:   44 DRERMHKSREHPQASRCIGVFGLNTNTSQHKVRELFNKYGPIERIQMVIDAQTQRSRGFCFIYFEKLSDARAAKDSCSGIEVDGRRIRVDFSITQRAHTPTPGVYLGRQ 152          
BLAST of Transformer 2 protein vs. Ensembl Fly
Match: tra2 (gene:FBgn0003742 transcript:FBtr0089619)

HSP 1 Score: 117.857 bits (294), Expect = 5.914e-30
Identity = 51/109 (46.79%), Postives = 77/109 (70.64%), Query Frame = 3
Query:  192 DKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTK 518
            D++   + R++P  S+C+G+F L+  T++  + E+F +YG IE + +VID  T+RSRGF F+YF+ + DA  AK+   G  V G++IRVD+S+T RAHTPTPGVY G +
Sbjct:   44 DRERMHKSREHPQASRCIGVFGLNTNTSQHKVRELFNKYGPIERIQMVIDAQTQRSRGFCFIYFEKLSDARAAKDSCSGIEVDGRRIRVDFSITQRAHTPTPGVYLGRQ 152          
BLAST of Transformer 2 protein vs. Ensembl Fly
Match: tra2 (gene:FBgn0003742 transcript:FBtr0089620)

HSP 1 Score: 117.857 bits (294), Expect = 1.439e-29
Identity = 51/109 (46.79%), Postives = 77/109 (70.64%), Query Frame = 3
Query:  192 DKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTK 518
            D++   + R++P  S+C+G+F L+  T++  + E+F +YG IE + +VID  T+RSRGF F+YF+ + DA  AK+   G  V G++IRVD+S+T RAHTPTPGVY G +
Sbjct:   82 DRERMHKSREHPQASRCIGVFGLNTNTSQHKVRELFNKYGPIERIQMVIDAQTQRSRGFCFIYFEKLSDARAAKDSCSGIEVDGRRIRVDFSITQRAHTPTPGVYLGRQ 190          
BLAST of Transformer 2 protein vs. Ensembl Fly
Match: tra2 (gene:FBgn0003742 transcript:FBtr0089621)

HSP 1 Score: 117.857 bits (294), Expect = 1.439e-29
Identity = 51/109 (46.79%), Postives = 77/109 (70.64%), Query Frame = 3
Query:  192 DKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTK 518
            D++   + R++P  S+C+G+F L+  T++  + E+F +YG IE + +VID  T+RSRGF F+YF+ + DA  AK+   G  V G++IRVD+S+T RAHTPTPGVY G +
Sbjct:   82 DRERMHKSREHPQASRCIGVFGLNTNTSQHKVRELFNKYGPIERIQMVIDAQTQRSRGFCFIYFEKLSDARAAKDSCSGIEVDGRRIRVDFSITQRAHTPTPGVYLGRQ 190          
BLAST of Transformer 2 protein vs. Ensembl Zebrafish
Match: tra2b (transformer 2 beta homolog [Source:ZFIN;Acc:ZDB-GENE-040426-1094])

HSP 1 Score: 135.191 bits (339), Expect = 1.245e-35
Identity = 61/116 (52.59%), Postives = 86/116 (74.14%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTHDIG 533
            MS+++    DR NP P+ CLG+F LSL TTE+DL E+F +YG + DV +V D+ ++RSRGF FVYF+N +D++ AKE+ +G  + G++IRVDYS+T R HTPTPG+Y G  T+  G
Sbjct:  106 MSNRRRHIGDRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPLSDVCIVYDQQSRRSRGFAFVYFENREDSKEAKERANGMELDGRRIRVDYSITKRPHTPTPGIYMGRPTYGGG 221          
BLAST of Transformer 2 protein vs. Ensembl Zebrafish
Match: tra2b (transformer 2 beta homolog [Source:ZFIN;Acc:ZDB-GENE-040426-1094])

HSP 1 Score: 135.191 bits (339), Expect = 1.245e-35
Identity = 61/116 (52.59%), Postives = 86/116 (74.14%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTHDIG 533
            MS+++    DR NP P+ CLG+F LSL TTE+DL E+F +YG + DV +V D+ ++RSRGF FVYF+N +D++ AKE+ +G  + G++IRVDYS+T R HTPTPG+Y G  T+  G
Sbjct:  106 MSNRRRHIGDRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPLSDVCIVYDQQSRRSRGFAFVYFENREDSKEAKERANGMELDGRRIRVDYSITKRPHTPTPGIYMGRPTYGGG 221          
BLAST of Transformer 2 protein vs. Ensembl Zebrafish
Match: tra2a (transformer 2 alpha homolog [Source:ZFIN;Acc:ZDB-GENE-040426-1391])

HSP 1 Score: 133.65 bits (335), Expect = 8.577e-35
Identity = 60/104 (57.69%), Postives = 79/104 (75.96%), Query Frame = 3
Query:  216 RDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTHD 527
            R NP P+ CLG+F LSL TTE+DL E+F RYG +  V++V D+ T RSRGF FVYF++IDDA+ A E+ +G  + G++IRVDYS+T R HTPTPG+Y G  TH+
Sbjct:  128 RANPDPNTCLGVFGLSLYTTERDLREVFSRYGSLAGVNVVYDQRTGRSRGFAFVYFEHIDDAKEAMERANGMELDGRRIRVDYSITKRPHTPTPGIYMGRPTHN 231          
BLAST of Transformer 2 protein vs. Ensembl Zebrafish
Match: tra2a (transformer 2 alpha homolog [Source:ZFIN;Acc:ZDB-GENE-040426-1391])

HSP 1 Score: 133.265 bits (334), Expect = 9.607e-35
Identity = 60/104 (57.69%), Postives = 79/104 (75.96%), Query Frame = 3
Query:  216 RDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTHD 527
            R NP P+ CLG+F LSL TTE+DL E+F RYG +  V++V D+ T RSRGF FVYF++IDDA+ A E+ +G  + G++IRVDYS+T R HTPTPG+Y G  TH+
Sbjct:  128 RANPDPNTCLGVFGLSLYTTERDLREVFSRYGSLAGVNVVYDQRTGRSRGFAFVYFEHIDDAKEAMERANGMELDGRRIRVDYSITKRPHTPTPGIYMGRPTHN 231          
BLAST of Transformer 2 protein vs. Ensembl Zebrafish
Match: tra2a (transformer 2 alpha homolog [Source:ZFIN;Acc:ZDB-GENE-040426-1391])

HSP 1 Score: 129.413 bits (324), Expect = 2.237e-34
Identity = 59/102 (57.84%), Postives = 77/102 (75.49%), Query Frame = 3
Query:  216 RDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKT 521
            R NP P+ CLG+F LSL TTE+DL E+F RYG +  V++V D+ T RSRGF FVYF++IDDA+ A E+ +G  + G++IRVDYS+T R HTPTPG+Y G  T
Sbjct:   99 RANPDPNTCLGVFGLSLYTTERDLREVFSRYGSLAGVNVVYDQRTGRSRGFAFVYFEHIDDAKEAMERANGMELDGRRIRVDYSITKRPHTPTPGIYMGRPT 200          
BLAST of Transformer 2 protein vs. Ensembl Xenopus
Match: rit1 (Ras-like without CAAX 1 [Source:Xenbase;Acc:XB-GENE-5777077])

HSP 1 Score: 138.272 bits (347), Expect = 4.496e-36
Identity = 62/113 (54.87%), Postives = 86/113 (76.11%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTH 524
            MS ++    +R NP P+ CLG+F LSL TTE+DL E+F +YG I DVS+V D+ ++RSRGF FVYF+N+DDA+ AKE+ +G  + G++IRVD+S+T R HTPTPG+Y G  T+
Sbjct:  136 MSTRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPISDVSIVYDQQSRRSRGFSFVYFENVDDAKEAKERANGMELDGRRIRVDFSITKRPHTPTPGIYMGRPTY 248          
BLAST of Transformer 2 protein vs. Ensembl Xenopus
Match: plekhg5 (pleckstrin homology and RhoGEF domain containing G5 [Source:Xenbase;Acc:XB-GENE-6039487])

HSP 1 Score: 131.339 bits (329), Expect = 6.300e-34
Identity = 60/113 (53.10%), Postives = 81/113 (71.68%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTH 524
            MS+++     R NP P+ C+G+F LSL TTE+DL E+F RYG +  V++V D+ T RSRGF FVYF+ I+D+  A E  +G  + G++IRVDYS+T RAHTPTPG+Y G  TH
Sbjct:   97 MSNRRRHNGSRANPDPNICIGVFGLSLYTTERDLREVFSRYGPLSGVNVVYDQRTGRSRGFAFVYFERIEDSREAMEHANGMELDGRRIRVDYSITKRAHTPTPGIYMGRPTH 209          
BLAST of Transformer 2 protein vs. Ensembl Xenopus
Match: cirbp (cold inducible RNA binding protein [Source:NCBI gene;Acc:549982])

HSP 1 Score: 72.4034 bits (176), Expect = 3.035e-14
Identity = 35/73 (47.95%), Postives = 53/73 (72.60%), Query Frame = 3
Query:  243 LGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVD 461
            L +  L+ ETTE+ L ++F +YG++ +V +V D+ +KRSRGFGFV F+N +DA+ A   ++G  V G+QIRVD
Sbjct:   20 LFVGGLNFETTEESLEQVFSKYGQVAEVVVVKDRESKRSRGFGFVTFENPEDAKDAMMAMNGKSVDGRQIRVD 92          
BLAST of Transformer 2 protein vs. Ensembl Xenopus
Match: cirbp (cold inducible RNA binding protein [Source:NCBI gene;Acc:549982])

HSP 1 Score: 72.4034 bits (176), Expect = 3.086e-14
Identity = 35/73 (47.95%), Postives = 53/73 (72.60%), Query Frame = 3
Query:  243 LGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVD 461
            L +  L+ ETTE+ L ++F +YG++ +V +V D+ +KRSRGFGFV F+N +DA+ A   ++G  V G+QIRVD
Sbjct:   20 LFVGGLNFETTEESLEQVFSKYGQVAEVVVVKDRESKRSRGFGFVTFENPEDAKDAMMAMNGKSVDGRQIRVD 92          
BLAST of Transformer 2 protein vs. Ensembl Xenopus
Match: cirbp (cold inducible RNA binding protein [Source:NCBI gene;Acc:549982])

HSP 1 Score: 72.0182 bits (175), Expect = 4.102e-14
Identity = 35/73 (47.95%), Postives = 53/73 (72.60%), Query Frame = 3
Query:  243 LGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVD 461
            L +  L+ ETTE+ L ++F +YG++ +V +V D+ +KRSRGFGFV F+N +DA+ A   ++G  V G+QIRVD
Sbjct:    8 LFVGGLNFETTEESLEQVFSKYGQVAEVVVVKDRESKRSRGFGFVTFENPEDAKDAMMAMNGKSVDGRQIRVD 80          
BLAST of Transformer 2 protein vs. Ensembl Mouse
Match: Tra2b (transformer 2 beta [Source:MGI Symbol;Acc:MGI:106016])

HSP 1 Score: 137.887 bits (346), Expect = 1.158e-37
Identity = 62/113 (54.87%), Postives = 86/113 (76.11%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTH 524
            MS ++    +R NP P+ CLG+F LSL TTE+DL E+F +YG I DVS+V D+ ++RSRGF FVYF+N+DDA+ AKE+ +G  + G++IRVD+S+T R HTPTPG+Y G  T+
Sbjct:    1 MSTRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKERANGMELDGRRIRVDFSITKRPHTPTPGIYMGRPTY 113          
BLAST of Transformer 2 protein vs. Ensembl Mouse
Match: Tra2b (transformer 2 beta [Source:MGI Symbol;Acc:MGI:106016])

HSP 1 Score: 138.272 bits (347), Expect = 1.575e-36
Identity = 62/113 (54.87%), Postives = 86/113 (76.11%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTH 524
            MS ++    +R NP P+ CLG+F LSL TTE+DL E+F +YG I DVS+V D+ ++RSRGF FVYF+N+DDA+ AKE+ +G  + G++IRVD+S+T R HTPTPG+Y G  T+
Sbjct:  101 MSTRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKERANGMELDGRRIRVDFSITKRPHTPTPGIYMGRPTY 213          
BLAST of Transformer 2 protein vs. Ensembl Mouse
Match: Tra2a (transformer 2 alpha [Source:MGI Symbol;Acc:MGI:1933972])

HSP 1 Score: 135.576 bits (340), Expect = 1.052e-35
Identity = 62/113 (54.87%), Postives = 83/113 (73.45%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTH 524
            MS+++     R NP P+ CLG+F LSL TTE+DL E+F RYG +  V++V D+ T RSRGF FVYF+ IDD++ A E+ +G  + G++IRVDYS+T RAHTPTPG+Y G  TH
Sbjct:  100 MSNRRRHTGSRANPDPNTCLGVFGLSLYTTERDLREVFSRYGPLSGVNVVYDQRTGRSRGFAFVYFERIDDSKEAMERANGMELDGRRIRVDYSITKRAHTPTPGIYMGRPTH 212          
BLAST of Transformer 2 protein vs. Ensembl Mouse
Match: Tra2a (transformer 2 alpha [Source:MGI Symbol;Acc:MGI:1933972])

HSP 1 Score: 135.576 bits (340), Expect = 1.104e-35
Identity = 62/113 (54.87%), Postives = 83/113 (73.45%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTH 524
            MS+++     R NP P+ CLG+F LSL TTE+DL E+F RYG +  V++V D+ T RSRGF FVYF+ IDD++ A E+ +G  + G++IRVDYS+T RAHTPTPG+Y G  TH
Sbjct:  100 MSNRRRHTGSRANPDPNTCLGVFGLSLYTTERDLREVFSRYGPLSGVNVVYDQRTGRSRGFAFVYFERIDDSKEAMERANGMELDGRRIRVDYSITKRAHTPTPGIYMGRPTH 212          
BLAST of Transformer 2 protein vs. Ensembl Mouse
Match: Cirbp (cold inducible RNA binding protein [Source:MGI Symbol;Acc:MGI:893588])

HSP 1 Score: 75.485 bits (184), Expect = 3.338e-16
Identity = 37/75 (49.33%), Postives = 54/75 (72.00%), Query Frame = 3
Query:  243 LGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYS 467
            L +  LS +T E+ L ++F +YG+I +V +V D+ T+RSRGFGFV F+NIDDA+ A   ++G  V G+QIRVD +
Sbjct:    8 LFVGGLSFDTNEQALEQVFSKYGQISEVVVVKDRETQRSRGFGFVTFENIDDAKDAMMAMNGKSVDGRQIRVDQA 82          
BLAST of Transformer 2 protein vs. UniProt/SwissProt
Match: sp|P62997|TRA2B_RAT (Transformer-2 protein homolog beta OS=Rattus norvegicus OX=10116 GN=Tra2b PE=1 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 1.101e-35
Identity = 62/113 (54.87%), Postives = 86/113 (76.11%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTH 524
            MS ++    +R NP P+ CLG+F LSL TTE+DL E+F +YG I DVS+V D+ ++RSRGF FVYF+N+DDA+ AKE+ +G  + G++IRVD+S+T R HTPTPG+Y G  T+
Sbjct:  101 MSTRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKERANGMELDGRRIRVDFSITKRPHTPTPGIYMGRPTY 213          
BLAST of Transformer 2 protein vs. UniProt/SwissProt
Match: sp|P62996|TRA2B_MOUSE (Transformer-2 protein homolog beta OS=Mus musculus OX=10090 GN=Tra2b PE=1 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 1.101e-35
Identity = 62/113 (54.87%), Postives = 86/113 (76.11%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTH 524
            MS ++    +R NP P+ CLG+F LSL TTE+DL E+F +YG I DVS+V D+ ++RSRGF FVYF+N+DDA+ AKE+ +G  + G++IRVD+S+T R HTPTPG+Y G  T+
Sbjct:  101 MSTRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKERANGMELDGRRIRVDFSITKRPHTPTPGIYMGRPTY 213          
BLAST of Transformer 2 protein vs. UniProt/SwissProt
Match: sp|P62995|TRA2B_HUMAN (Transformer-2 protein homolog beta OS=Homo sapiens OX=9606 GN=TRA2B PE=1 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 1.101e-35
Identity = 62/113 (54.87%), Postives = 86/113 (76.11%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTH 524
            MS ++    +R NP P+ CLG+F LSL TTE+DL E+F +YG I DVS+V D+ ++RSRGF FVYF+N+DDA+ AKE+ +G  + G++IRVD+S+T R HTPTPG+Y G  T+
Sbjct:  101 MSTRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKERANGMELDGRRIRVDFSITKRPHTPTPGIYMGRPTY 213          
BLAST of Transformer 2 protein vs. UniProt/SwissProt
Match: sp|Q3ZBT6|TRA2B_BOVIN (Transformer-2 protein homolog beta OS=Bos taurus OX=9913 GN=TRA2B PE=2 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 1.101e-35
Identity = 62/113 (54.87%), Postives = 86/113 (76.11%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTH 524
            MS ++    +R NP P+ CLG+F LSL TTE+DL E+F +YG I DVS+V D+ ++RSRGF FVYF+N+DDA+ AKE+ +G  + G++IRVD+S+T R HTPTPG+Y G  T+
Sbjct:  101 MSTRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKERANGMELDGRRIRVDFSITKRPHTPTPGIYMGRPTY 213          
BLAST of Transformer 2 protein vs. UniProt/SwissProt
Match: sp|Q6PFR5|TRA2A_MOUSE (Transformer-2 protein homolog alpha OS=Mus musculus OX=10090 GN=Tra2a PE=1 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 7.539e-35
Identity = 62/113 (54.87%), Postives = 83/113 (73.45%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTH 524
            MS+++     R NP P+ CLG+F LSL TTE+DL E+F RYG +  V++V D+ T RSRGF FVYF+ IDD++ A E+ +G  + G++IRVDYS+T RAHTPTPG+Y G  TH
Sbjct:  100 MSNRRRHTGSRANPDPNTCLGVFGLSLYTTERDLREVFSRYGPLSGVNVVYDQRTGRSRGFAFVYFERIDDSKEAMERANGMELDGRRIRVDYSITKRAHTPTPGIYMGRPTH 212          
BLAST of Transformer 2 protein vs. TrEMBL
Match: A0A0R3WII9 (RRM domain-containing protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS418 PE=4 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 7.068e-36
Identity = 63/108 (58.33%), Postives = 82/108 (75.93%), Query Frame = 3
Query:  210 EDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTHDIG 533
            + RDNP+PS+C+G+F LSL TTE+DL+++F RYGE+EDV++V D  T  SRGFGFVY +++ DA+ AK   HGT + G+ IRVDYSVT R H+PTPGVY G  T   G
Sbjct:   80 QPRDNPVPSRCIGVFGLSLSTTERDLYDLFSRYGEVEDVTIVYDNFTCHSRGFGFVYMRHLSDAKEAKHDAHGTELDGRPIRVDYSVTERPHSPTPGVYMGRPTRRCG 187          
BLAST of Transformer 2 protein vs. TrEMBL
Match: A0A1I8FUT4 (RRM domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 1.490e-35
Identity = 66/110 (60.00%), Postives = 81/110 (73.64%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGT 515
            MS +K    DRDNP  SKCLG+F LSL TTE+DL  +F RYG +EDV LV D  T+RSRGF FVYF+++DDA  AKE+ HG  + G+ +R D+S+T RAHTPTPGVY G 
Sbjct:   58 MSSRKRHVGDRDNPKASKCLGVFGLSLRTTEEDLRHLFGRYGRLEDVQLVKDNHTRRSRGFAFVYFESLDDATYAKERCHGKELDGRNMRCDFSITRRAHTPTPGVYLGA 167          
BLAST of Transformer 2 protein vs. TrEMBL
Match: A0A068Y440 (Transformer 2 protein OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000709100 PE=4 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 1.494e-35
Identity = 63/108 (58.33%), Postives = 82/108 (75.93%), Query Frame = 3
Query:  210 EDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTHDIG 533
            + RDNP+PS+C+G+F LSL TTE+DL+++F RYGE+EDV++V D  T  SRGFGFVY +++ DA+ AK   HGT + G+ IRVDYSVT R H+PTPGVY G  T   G
Sbjct:   92 QPRDNPVPSRCIGVFGLSLSTTERDLYDLFSRYGEVEDVTIVYDNFTGHSRGFGFVYMRHLSDAKEAKHDAHGTELDGRPIRVDYSVTERPHSPTPGVYMGRPTRRCG 199          
BLAST of Transformer 2 protein vs. TrEMBL
Match: A0A068WSF6 (Transformer 2 protein OS=Echinococcus granulosus OX=6210 GN=EgrG_000709100 PE=4 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 1.494e-35
Identity = 63/108 (58.33%), Postives = 82/108 (75.93%), Query Frame = 3
Query:  210 EDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTHDIG 533
            + RDNP+PS+C+G+F LSL TTE+DL+++F RYGE+EDV++V D  T  SRGFGFVY +++ DA+ AK   HGT + G+ IRVDYSVT R H+PTPGVY G  T   G
Sbjct:   92 QPRDNPVPSRCIGVFGLSLSTTERDLYDLFSRYGEVEDVTIVYDNFTGHSRGFGFVYMRHLSDAKEAKHDAHGTELDGRPIRVDYSVTERPHSPTPGVYMGRPTRRCG 199          
BLAST of Transformer 2 protein vs. TrEMBL
Match: A0A3P6QF40 (RRM domain-containing protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS7685 PE=4 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 4.225e-35
Identity = 63/108 (58.33%), Postives = 82/108 (75.93%), Query Frame = 3
Query:  210 EDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTHDIG 533
            + RDNP+PS+C+G+F LSL TTE+DL+++F RYGE+EDV++V D  T  SRGFGFVY +++ DA+ AK   HGT + G+ IRVDYSVT R H+PTPGVY G  T   G
Sbjct:  112 QPRDNPVPSRCIGVFGLSLSTTERDLYDLFSRYGEVEDVTIVYDNFTCHSRGFGFVYMRHLSDAKEAKHDAHGTELDGRPIRVDYSVTERPHSPTPGVYMGRPTRRCG 219          
BLAST of Transformer 2 protein vs. Ensembl Cavefish
Match: ENSAMXT00000034164.1 (pep primary_assembly:Astyanax_mexicanus-2.0:12:6544829:6548035:1 gene:ENSAMXG00000039469.1 transcript:ENSAMXT00000034164.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 134.035 bits (336), Expect = 2.119e-36
Identity = 60/116 (51.72%), Postives = 85/116 (73.28%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTHDIG 533
            MS+++    +R NP P+ CLG+F LSL TTE+DL E+F +YG +  VS+V D+ ++RSRGF FVYF+N +D+  AKE+ +G  + G++IRVDYS+T R HTPTPG+Y G  T+  G
Sbjct:    1 MSNRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPLSSVSIVYDQQSRRSRGFAFVYFENREDSREAKERANGMELDGRRIRVDYSITQRPHTPTPGIYMGRPTYGGG 116          
BLAST of Transformer 2 protein vs. Ensembl Cavefish
Match: tra2a (transformer 2 alpha homolog [Source:ZFIN;Acc:ZDB-GENE-040426-1391])

HSP 1 Score: 131.724 bits (330), Expect = 1.100e-34
Identity = 60/113 (53.10%), Postives = 81/113 (71.68%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTH 524
            MS+++     R NP P+ CLG+F LSL TTE+DL E+F RYG +  V++V D+ T RSRGF FVYF+ IDD++ A E+ +G  + G++IRVDYS+T R HTPTPG+Y G  T 
Sbjct:   90 MSNRRRHAGSRANPDPNTCLGVFGLSLYTTERDLREVFSRYGPLAGVNVVYDQRTGRSRGFAFVYFERIDDSKEAMERANGMELDGRRIRVDYSITKRPHTPTPGIYMGRPTQ 202          
BLAST of Transformer 2 protein vs. Ensembl Cavefish
Match: tra2b (transformer-2 protein homolog alpha-like [Source:NCBI gene;Acc:103029311])

HSP 1 Score: 132.109 bits (331), Expect = 2.460e-34
Identity = 58/113 (51.33%), Postives = 85/113 (75.22%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTH 524
            MS+++    +R NP P+ CLG+F LSL TTE+DL E+F +YG + DV +V D+ ++RSRGF FVYF+N +D++ AKE+ +G  + G++IRVD+S+T R HTPTPG+Y G  T+
Sbjct:  101 MSNRRRHIGNRANPDPTCCLGVFGLSLYTTERDLREVFSKYGPLADVCIVYDQQSRRSRGFAFVYFENREDSQEAKERANGMELDGRRIRVDFSITKRPHTPTPGIYMGRPTY 213          
BLAST of Transformer 2 protein vs. Ensembl Cavefish
Match: tra2a (transformer 2 alpha homolog [Source:ZFIN;Acc:ZDB-GENE-040426-1391])

HSP 1 Score: 127.872 bits (320), Expect = 1.628e-33
Identity = 58/103 (56.31%), Postives = 76/103 (73.79%), Query Frame = 3
Query:  216 RDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTH 524
            R NP P+ CLG+F LSL TTE+DL E+F RYG +  V++V D+ T RSRGF FVYF+ IDD++ A E+ +G  + G++IRVDYS+T R HTPTPG+Y G  T 
Sbjct:  128 RANPDPNTCLGVFGLSLYTTERDLREVFSRYGPLAGVNVVYDQRTGRSRGFAFVYFERIDDSKEAMERANGMELDGRRIRVDYSITKRPHTPTPGIYMGRPTQ 230          
BLAST of Transformer 2 protein vs. Ensembl Cavefish
Match: cirbpa (cold inducible RNA binding protein a [Source:ZFIN;Acc:ZDB-GENE-050417-329])

HSP 1 Score: 69.707 bits (169), Expect = 9.416e-14
Identity = 37/73 (50.68%), Postives = 49/73 (67.12%), Query Frame = 3
Query:  243 LGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVD 461
            L I  LS +TTE+ L E F +YG I +V +  ++ T RSRGFGFV F+N DDA+ A E ++G  V G+ IRVD
Sbjct:    7 LFIGGLSFDTTEQSLEEAFSKYGVITNVHVARNRETNRSRGFGFVTFENPDDAKDALEGMNGKSVDGRAIRVD 79          
BLAST of Transformer 2 protein vs. Ensembl Sea Lamprey
Match: tra2b (transformer 2 beta homolog [Source:ZFIN;Acc:ZDB-GENE-040426-1094])

HSP 1 Score: 131.339 bits (329), Expect = 7.036e-35
Identity = 58/109 (53.21%), Postives = 82/109 (75.23%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKG 512
             S+++  + DR NP P+ CLG+F LSL TTE DL E+F RYG + DV++V D+ + R RGF FVY +N++D++ AKE+V+G  + G+ IRVD+S+T RAHTPTPG+Y G
Sbjct:   89 FSNRRRHQGDRGNPEPNTCLGVFGLSLYTTEHDLREVFGRYGPVADVNVVYDQQSGRPRGFAFVYMQNMEDSKEAKERVNGMELDGRNIRVDFSITKRAHTPTPGIYMG 197          
BLAST of Transformer 2 protein vs. Ensembl Sea Lamprey
Match: tra2a (transformer 2 alpha homolog [Source:ZFIN;Acc:ZDB-GENE-040426-1391])

HSP 1 Score: 130.183 bits (326), Expect = 1.372e-34
Identity = 62/118 (52.54%), Postives = 83/118 (70.34%), Query Frame = 3
Query:  168 RSNIVTMSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKT 521
            R +   MS+++     R NP PS CLG+F LSL T EKDL E+F RYG ++ V++V D+ + RSRGF FVYF N +DA+ AKE+ +G  + G+ IRVD+S+T RAHTPTPG+Y G  T
Sbjct:   89 RHSRSPMSNRRRHLGSRVNPDPSCCLGVFGLSLHTGEKDLKEVFSRYGPVQSVNVVYDQQSGRSRGFAFVYFDNSEDAKEAKERANGMELDGRYIRVDFSITKRAHTPTPGIYMGRPT 206          
BLAST of Transformer 2 protein vs. Ensembl Sea Lamprey
Match: rbmx (RNA binding motif protein X-linked [Source:NCBI gene;Acc:321860])

HSP 1 Score: 67.3958 bits (163), Expect = 2.250e-12
Identity = 30/71 (42.25%), Postives = 47/71 (66.20%), Query Frame = 3
Query:  249 IFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVD 461
            +  L+++TTEK L   F +YG + DV L+ D+ T  SRGF FV F N +DA+ A+   +G +++G+ +RVD
Sbjct:   11 VGGLNIDTTEKALEATFSKYGRVADVLLMKDRETNLSRGFAFVTFANPEDAKEAERDANGKVLEGRAMRVD 81          
BLAST of Transformer 2 protein vs. Ensembl Sea Lamprey
Match: srsf2b (serine and arginine rich splicing factor 2b [Source:ZFIN;Acc:ZDB-GENE-030131-2420])

HSP 1 Score: 63.1586 bits (152), Expect = 1.209e-11
Identity = 31/87 (35.63%), Postives = 51/87 (58.62%), Query Frame = 3
Query:  231 PSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTP 491
            PS+ L + NL+  T+ + L   F +YG++ DV +  D+ T+ SRGF FV F +  DAE A + + G ++ G+++RV  +   R   P
Sbjct:    6 PSRALQVDNLTYRTSPEMLRRSFEKYGDVGDVYIPRDRFTRESRGFAFVRFYDKRDAEDAMDAMDGKMLDGRELRVQVARYSRPADP 92          
BLAST of Transformer 2 protein vs. Ensembl Sea Lamprey
Match: rbmx (RNA binding motif protein X-linked [Source:NCBI gene;Acc:321860])

HSP 1 Score: 62.7734 bits (151), Expect = 5.670e-11
Identity = 31/77 (40.26%), Postives = 47/77 (61.04%), Query Frame = 3
Query:  231 PSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVD 461
            P K   I  LS +T E  L  +F +YG + +V ++ D+ T RSRGF FV F N DDA+ A+  ++G  + G+ I+V+
Sbjct:    6 PGKLF-IGGLSTDTNENALETIFSKYGRVAEVLIMKDRETNRSRGFAFVTFANPDDAKGAERDMNGKPLDGRNIKVE 81          
BLAST of Transformer 2 protein vs. Ensembl Yeast
Match: NSR1 (Nucleolar protein that binds nuclear localization sequences; required for pre-rRNA processing and ribosome biogenesis; binds to single stranded telomeric DNA and mRNA; methylated by Hmt1p; interaction with Top1p and nucleolar localization are negatively regulated by polyphosphorylation [Source:SGD;Acc:S000003391])

HSP 1 Score: 56.225 bits (134), Expect = 5.979e-9
Identity = 25/79 (31.65%), Postives = 46/79 (58.23%), Query Frame = 3
Query:  231 PSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYS 467
            PS  L + NLS       + E+F ++GE+  V +     T++ +GFG+V F N++DA+ A + + G  +  + +R+D+S
Sbjct:  265 PSDTLFLGNLSFNADRDAIFELFAKHGEVVSVRIPTHPETEQPKGFGYVQFSNMEDAKKALDALQGEYIDNRPVRLDFS 343          
BLAST of Transformer 2 protein vs. Ensembl Yeast
Match: NAM8 (RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function [Source:SGD;Acc:S000001128])

HSP 1 Score: 53.5286 bits (127), Expect = 5.477e-8
Identity = 25/68 (36.76%), Postives = 39/68 (57.35%), Query Frame = 3
Query:  258 LSLETTEKDLHEMFF-RYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRV 458
            L+   TE  L E+F  RY       +V D+VT  S+G+GFV F N D+ ++A  ++ G  + G+ I+V
Sbjct:  170 LAPNVTESQLFELFINRYASTSHAKIVHDQVTGMSKGYGFVKFTNSDEQQLALSEMQGVFLNGRAIKV 237          
BLAST of Transformer 2 protein vs. Ensembl Yeast
Match: MIP6 (Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication [Source:SGD;Acc:S000001057])

HSP 1 Score: 49.6766 bits (117), Expect = 9.386e-7
Identity = 25/72 (34.72%), Postives = 46/72 (63.89%), Query Frame = 3
Query:  243 LGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRV 458
            L I NL    TE+ L ++F RY   E   +  D +TK+S G+G++ FK+ +DAE A+++ + T+  G+++++
Sbjct:  113 LFIGNLKSTVTEEMLRKIFKRYQSFESAKVCRDFLTKKSLGYGYLNFKDKNDAESARKEFNYTVFFGQEVKI 184          
BLAST of Transformer 2 protein vs. Ensembl Yeast
Match: HRP1 (Subunit of cleavage factor I; cleavage factor I is a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences; required for genome stability [Source:SGD;Acc:S000005483])

HSP 1 Score: 48.521 bits (114), Expect = 1.609e-6
Identity = 21/46 (45.65%), Postives = 31/46 (67.39%), Query Frame = 3
Query:  249 IFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFK 386
            I  L+ +TTE +L E F +YG + D+ ++ D  T RSRGFGF+ F+
Sbjct:  163 IGGLNWDTTEDNLREYFGKYGTVTDLKIMKDPATGRSRGFGFLSFE 208          
BLAST of Transformer 2 protein vs. Ensembl Yeast
Match: NOP15 (Constituent of 66S pre-ribosomal particles; involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm [Source:SGD;Acc:S000005054])

HSP 1 Score: 46.595 bits (109), Expect = 2.722e-6
Identity = 24/63 (38.10%), Postives = 42/63 (66.67%), Query Frame = 3
Query:  276 EKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGK--QIRV 458
            EK+L + F ++G++++V L  +K T  SR +GF+ F N +DA IA+E ++  ++ G   Q+RV
Sbjct:  104 EKELSKYFAQFGDLKEVRLARNKKTGNSRHYGFLEFVNKEDAMIAQESMNNYLLMGHLLQVRV 166          
BLAST of Transformer 2 protein vs. Ensembl Nematostella
Match: EDO44802 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUS7])

HSP 1 Score: 139.428 bits (350), Expect = 1.276e-38
Identity = 67/123 (54.47%), Postives = 89/123 (72.36%), Query Frame = 3
Query:  150 NPFEPYRSNIV--TMSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKG 512
            NP+   R N     +S++K  + +RDNP PS+CLG+F LSL TTE+DL  +F +YG +E + +V D  T RSRGFGFVY+++  DAE+AKE+  G  + G++IRVDYSVT RAHTPTPGVY G
Sbjct:   64 NPYYKGRRNSSHSPLSNRKRHQGNRDNPSPSRCLGVFGLSLYTTERDLRPVFEKYGPVEAIQIVYDHQTGRSRGFGFVYYEDTKDAEVAKERTTGIELDGRRIRVDYSVTKRAHTPTPGVYMG 186          
BLAST of Transformer 2 protein vs. Ensembl Nematostella
Match: EDO44890 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUE7])

HSP 1 Score: 55.4546 bits (132), Expect = 5.004e-10
Identity = 31/83 (37.35%), Postives = 51/83 (61.45%), Query Frame = 3
Query:  210 EDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRV 458
            E+ D+  P K L + N+     + DL +MF  +G I DV ++ ++  + S+GFGFV F    +A  A+EK++GTIV G+++ V
Sbjct:    2 ENGDSSGP-KRLHVTNIPFRFRDNDLRQMFGSFGVIADVEIIYNE--RGSKGFGFVTFNTAAEANKAREKLNGTIVDGRKVEV 81          
BLAST of Transformer 2 protein vs. Ensembl Nematostella
Match: EDO39952 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8K1])

HSP 1 Score: 55.8398 bits (133), Expect = 2.551e-9
Identity = 30/77 (38.96%), Postives = 46/77 (59.74%), Query Frame = 3
Query:  234 SKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDY 464
            S  L + NLS  T+E+ ++E+F R GE++ + + +DKV K   GF FV +    DAE A   ++GT +  + IR DY
Sbjct:   26 SSTLYVGNLSFYTSEQQIYELFGRAGELKKIIMGLDKVRKTPCGFCFVEYYTRPDAENAMRYINGTRLDQRIIRTDY 102          
BLAST of Transformer 2 protein vs. Ensembl Nematostella
Match: EDO49282 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RH27])

HSP 1 Score: 53.5286 bits (127), Expect = 3.761e-9
Identity = 27/81 (33.33%), Postives = 47/81 (58.02%), Query Frame = 3
Query:  231 PSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVY--FKNIDDAEIAKEKVHGTIVQGKQIRVDYS 467
            P+  + + NL  +T   DL  MF +YG I DV + +D  T+  RGF ++Y  F++I DAE A   +   ++ G+++ V ++
Sbjct:    8 PNSSIYVRNLHPDTRPDDLRRMFGKYGRITDVYIPLDYYTREPRGFCYIYITFEDIRDAEDALYYLDRAMLLGRELEVQFA 88          
BLAST of Transformer 2 protein vs. Ensembl Nematostella
Match: EDO29715 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T2V2])

HSP 1 Score: 51.9878 bits (123), Expect = 8.604e-9
Identity = 27/71 (38.03%), Postives = 42/71 (59.15%), Query Frame = 3
Query:  255 NLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYS 467
            +L     E DL   F  YG ++ V ++ DK T RS+GFGFV   N  +A+ A ++++G  V+G+ I V+ S
Sbjct:    7 SLPFSIEEADLRGFFEEYGAVDSVKIISDKFTGRSKGFGFVEMPNDAEAQKAIDELNGGTVEGRTIVVNKS 77          
BLAST of Transformer 2 protein vs. Ensembl Medaka
Match: tra2a (transformer 2a [Source:NCBI gene;Acc:100049284])

HSP 1 Score: 133.265 bits (334), Expect = 6.178e-35
Identity = 59/113 (52.21%), Postives = 87/113 (76.99%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTH 524
            MS+++    +R NP PS CLG+F LSL TTE+DL E+F +YG + +V++V D+ ++RSRGF FVYF+N +D++ AKE+ +G  + G++IRVD+S+T RAHTPTPG+Y G  T+
Sbjct:   98 MSNRRRHIGNRGNPDPSCCLGVFGLSLYTTERDLREVFSKYGPLSEVNIVYDQQSRRSRGFAFVYFENSEDSKEAKEQANGMELDGRRIRVDFSITKRAHTPTPGIYMGRPTY 210          
BLAST of Transformer 2 protein vs. Ensembl Medaka
Match: tra2a (transformer-2 protein homolog alpha [Source:NCBI gene;Acc:101157868])

HSP 1 Score: 130.954 bits (328), Expect = 2.947e-34
Identity = 59/118 (50.00%), Postives = 81/118 (68.64%), Query Frame = 3
Query:  174 NIVTMSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTHD 527
            N   MS ++     R+NP PS CLG+F L+L TTE+DL E+F RYG +  +++V D+ T RSRGF F+YF+ + DA  A E  +G  + G+QIRVDYS+T R HTPTPG+Y G  T++
Sbjct:   88 NASPMSSRRRHTGTRENPDPSTCLGVFGLNLSTTERDLREVFSRYGRLTGINMVHDQRTGRSRGFAFIYFERLCDAREAIEHTNGMNMDGRQIRVDYSITKRPHTPTPGIYMGRPTYN 205          
BLAST of Transformer 2 protein vs. Ensembl Medaka
Match: tra2b (transformer 2 beta homolog [Source:NCBI gene;Acc:100049285])

HSP 1 Score: 129.413 bits (324), Expect = 1.632e-33
Identity = 56/98 (57.14%), Postives = 77/98 (78.57%), Query Frame = 3
Query:  213 DRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVY 506
            +R NP P+ CLG+F LSL TTE+DL ++F +YG + DVS+V D+ ++RSRGF FVYF+N  DA+ AKEK +G  + G++IRVD+S+T R HTPTPG+Y
Sbjct:  114 NRANPDPNACLGVFGLSLYTTERDLKDVFSKYGPLADVSIVYDQQSRRSRGFAFVYFENTPDAKEAKEKANGMELDGRRIRVDFSITKRPHTPTPGIY 211          
BLAST of Transformer 2 protein vs. Ensembl Medaka
Match: tra2b (transformer 2 beta homolog [Source:NCBI gene;Acc:100049285])

HSP 1 Score: 129.028 bits (323), Expect = 2.679e-33
Identity = 56/98 (57.14%), Postives = 77/98 (78.57%), Query Frame = 3
Query:  213 DRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVY 506
            +R NP P+ CLG+F LSL TTE+DL ++F +YG + DVS+V D+ ++RSRGF FVYF+N  DA+ AKEK +G  + G++IRVD+S+T R HTPTPG+Y
Sbjct:  123 NRANPDPNACLGVFGLSLYTTERDLKDVFSKYGPLADVSIVYDQQSRRSRGFAFVYFENTPDAKEAKEKANGMELDGRRIRVDFSITKRPHTPTPGIY 220          
BLAST of Transformer 2 protein vs. Ensembl Medaka
Match: tra2b (transformer 2 beta homolog [Source:NCBI gene;Acc:100049285])

HSP 1 Score: 127.872 bits (320), Expect = 6.650e-33
Identity = 56/98 (57.14%), Postives = 77/98 (78.57%), Query Frame = 3
Query:  213 DRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVY 506
            +R NP P+ CLG+F LSL TTE+DL ++F +YG + DVS+V D+ ++RSRGF FVYF+N  DA+ AKEK +G  + G++IRVD+S+T R HTPTPG+Y
Sbjct:  109 NRANPDPNACLGVFGLSLYTTERDLKDVFSKYGPLADVSIVYDQQSRRSRGFAFVYFENTPDAKEAKEKANGMELDGRRIRVDFSITKRPHTPTPGIY 206          
BLAST of Transformer 2 protein vs. Planmine SMEST
Match: SMESG000022369.1 (SMESG000022369.1)

HSP 1 Score: 294.664 bits (753), Expect = 5.350e-98
Identity = 187/187 (100.00%), Postives = 187/187 (100.00%), Query Frame = 3
Query:  186 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTHDIGXXXXXXXXXXXXXXXXXXXXXXXEQRMDSRYRSGESFXXXXXXXXXXXXXXXXWTDYPPRRRSPFNDRPHY 746
            MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTHDIGRKPNKSRSNRRNPRDRLSDRDRSEQRMDSRYRSGESFDRRGGRYEYSERRSRDWTDYPPRRRSPFNDRPHY
Sbjct:    1 MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKTHDIGRKPNKSRSNRRNPRDRLSDRDRSEQRMDSRYRSGESFDRRGGRYEYSERRSRDWTDYPPRRRSPFNDRPHY 187          
BLAST of Transformer 2 protein vs. Planmine SMEST
Match: SMESG000031639.1 (SMESG000031639.1)

HSP 1 Score: 136.346 bits (342), Expect = 7.760e-37
Identity = 59/103 (57.28%), Postives = 80/103 (77.67%), Query Frame = 3
Query:  213 DRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKT 521
            DRDNP PS C+G+F LSL+T EKDL+ +F +YGEI+DV LV+D  T RSRGFGF+Y++ ++ A  AK+  HG  + G+ +R+DYS+T +AHTPTPGVY G K+
Sbjct:   79 DRDNPKPSTCIGVFGLSLKTDEKDLYRVFQKYGEIKDVQLVLDNYTGRSRGFGFIYYEEVESARNAKDSAHGIEIDGRVVRIDYSITRKAHTPTPGVYLGKKS 181          
BLAST of Transformer 2 protein vs. Planmine SMEST
Match: SMESG000031639.1 (SMESG000031639.1)

HSP 1 Score: 136.346 bits (342), Expect = 8.053e-37
Identity = 59/103 (57.28%), Postives = 80/103 (77.67%), Query Frame = 3
Query:  213 DRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKT 521
            DRDNP PS C+G+F LSL+T EKDL+ +F +YGEI+DV LV+D  T RSRGFGF+Y++ ++ A  AK+  HG  + G+ +R+DYS+T +AHTPTPGVY G K+
Sbjct:   80 DRDNPKPSTCIGVFGLSLKTDEKDLYRVFQKYGEIKDVQLVLDNYTGRSRGFGFIYYEEVESARNAKDSAHGIEIDGRVVRIDYSITRKAHTPTPGVYLGKKS 182          
BLAST of Transformer 2 protein vs. Planmine SMEST
Match: SMESG000031644.1 (SMESG000031644.1)

HSP 1 Score: 135.961 bits (341), Expect = 1.507e-36
Identity = 59/103 (57.28%), Postives = 80/103 (77.67%), Query Frame = 3
Query:  213 DRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKT 521
            DRDNP PS C+G+F LSL+T EKDL+ +F +YGEI+DV LV+D  T RSRGFGF+Y++ ++ A  AK+  HG  + G+ +R+DYS+T +AHTPTPGVY G K+
Sbjct:   79 DRDNPKPSTCIGVFGLSLKTDEKDLYRVFQKYGEIKDVQLVLDNYTGRSRGFGFIYYEEVESARNAKDSAHGIEIDGRVVRIDYSITRKAHTPTPGVYLGKKS 181          
BLAST of Transformer 2 protein vs. Planmine SMEST
Match: SMESG000031644.1 (SMESG000031644.1)

HSP 1 Score: 135.576 bits (340), Expect = 1.596e-36
Identity = 59/103 (57.28%), Postives = 80/103 (77.67%), Query Frame = 3
Query:  213 DRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLVIDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAHTPTPGVYKGTKT 521
            DRDNP PS C+G+F LSL+T EKDL+ +F +YGEI+DV LV+D  T RSRGFGF+Y++ ++ A  AK+  HG  + G+ +R+DYS+T +AHTPTPGVY G K+
Sbjct:   80 DRDNPKPSTCIGVFGLSLKTDEKDLYRVFQKYGEIKDVQLVLDNYTGRSRGFGFIYYEEVESARNAKDSAHGIEIDGRVVRIDYSITRKAHTPTPGVYLGKKS 182          
The following BLAST results are available for this feature:
BLAST of Transformer 2 protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TRA2B1.686e-3754.87transformer 2 beta homolog [Source:HGNC Symbol;Acc... [more]
TRA2A1.362e-3654.87transformer 2 alpha homolog [Source:HGNC Symbol;Ac... [more]
TRA2A1.402e-3654.87transformer 2 alpha homolog [Source:HGNC Symbol;Ac... [more]
TRA2A1.402e-3654.87transformer 2 alpha homolog [Source:HGNC Symbol;Ac... [more]
TRA2B2.294e-3654.87transformer 2 beta homolog [Source:HGNC Symbol;Acc... [more]
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BLAST of Transformer 2 protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rsp-85.520e-3056.12SR Protein (Splicing factor) [Source:UniProtKB/Tr... [more]
rsp-85.520e-3056.12SR Protein (Splicing factor) [Source:UniProtKB/Tr... [more]
rsp-43.450e-1346.58Probable splicing factor, arginine/serine-rich 4 ... [more]
rsp-41.249e-1246.58Probable splicing factor, arginine/serine-rich 4 ... [more]
fox-12.054e-931.43Sex determination protein fox-1 [Source:UniProtKB... [more]
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BLAST of Transformer 2 protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tra22.635e-3048.54gene:FBgn0003742 transcript:FBtr0089616[more]
tra25.914e-3046.79gene:FBgn0003742 transcript:FBtr0089615[more]
tra25.914e-3046.79gene:FBgn0003742 transcript:FBtr0089619[more]
tra21.439e-2946.79gene:FBgn0003742 transcript:FBtr0089620[more]
tra21.439e-2946.79gene:FBgn0003742 transcript:FBtr0089621[more]
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BLAST of Transformer 2 protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tra2b1.245e-3552.59transformer 2 beta homolog [Source:ZFIN;Acc:ZDB-GE... [more]
tra2b1.245e-3552.59transformer 2 beta homolog [Source:ZFIN;Acc:ZDB-GE... [more]
tra2a8.577e-3557.69transformer 2 alpha homolog [Source:ZFIN;Acc:ZDB-G... [more]
tra2a9.607e-3557.69transformer 2 alpha homolog [Source:ZFIN;Acc:ZDB-G... [more]
tra2a2.237e-3457.84transformer 2 alpha homolog [Source:ZFIN;Acc:ZDB-G... [more]
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BLAST of Transformer 2 protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rit14.496e-3654.87Ras-like without CAAX 1 [Source:Xenbase;Acc:XB-GEN... [more]
plekhg56.300e-3453.10pleckstrin homology and RhoGEF domain containing G... [more]
cirbp3.035e-1447.95cold inducible RNA binding protein [Source:NCBI ge... [more]
cirbp3.086e-1447.95cold inducible RNA binding protein [Source:NCBI ge... [more]
cirbp4.102e-1447.95cold inducible RNA binding protein [Source:NCBI ge... [more]
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BLAST of Transformer 2 protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tra2b1.158e-3754.87transformer 2 beta [Source:MGI Symbol;Acc:MGI:1060... [more]
Tra2b1.575e-3654.87transformer 2 beta [Source:MGI Symbol;Acc:MGI:1060... [more]
Tra2a1.052e-3554.87transformer 2 alpha [Source:MGI Symbol;Acc:MGI:193... [more]
Tra2a1.104e-3554.87transformer 2 alpha [Source:MGI Symbol;Acc:MGI:193... [more]
Cirbp3.338e-1649.33cold inducible RNA binding protein [Source:MGI Sym... [more]
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BLAST of Transformer 2 protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P62997|TRA2B_RAT1.101e-3554.87Transformer-2 protein homolog beta OS=Rattus norve... [more]
sp|P62996|TRA2B_MOUSE1.101e-3554.87Transformer-2 protein homolog beta OS=Mus musculus... [more]
sp|P62995|TRA2B_HUMAN1.101e-3554.87Transformer-2 protein homolog beta OS=Homo sapiens... [more]
sp|Q3ZBT6|TRA2B_BOVIN1.101e-3554.87Transformer-2 protein homolog beta OS=Bos taurus O... [more]
sp|Q6PFR5|TRA2A_MOUSE7.539e-3554.87Transformer-2 protein homolog alpha OS=Mus musculu... [more]
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BLAST of Transformer 2 protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0R3WII97.068e-3658.33RRM domain-containing protein OS=Hydatigena taenia... [more]
A0A1I8FUT41.490e-3560.00RRM domain-containing protein OS=Macrostomum ligna... [more]
A0A068Y4401.494e-3558.33Transformer 2 protein OS=Echinococcus multilocular... [more]
A0A068WSF61.494e-3558.33Transformer 2 protein OS=Echinococcus granulosus O... [more]
A0A3P6QF404.225e-3558.33RRM domain-containing protein OS=Taenia asiatica O... [more]
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BLAST of Transformer 2 protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000034164.12.119e-3651.72pep primary_assembly:Astyanax_mexicanus-2.0:12:654... [more]
tra2a1.100e-3453.10transformer 2 alpha homolog [Source:ZFIN;Acc:ZDB-G... [more]
tra2b2.460e-3451.33transformer-2 protein homolog alpha-like [Source:N... [more]
tra2a1.628e-3356.31transformer 2 alpha homolog [Source:ZFIN;Acc:ZDB-G... [more]
cirbpa9.416e-1450.68cold inducible RNA binding protein a [Source:ZFIN;... [more]
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BLAST of Transformer 2 protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tra2b7.036e-3553.21transformer 2 beta homolog [Source:ZFIN;Acc:ZDB-GE... [more]
tra2a1.372e-3452.54transformer 2 alpha homolog [Source:ZFIN;Acc:ZDB-G... [more]
rbmx2.250e-1242.25RNA binding motif protein X-linked [Source:NCBI ge... [more]
srsf2b1.209e-1135.63serine and arginine rich splicing factor 2b [Sourc... [more]
rbmx5.670e-1140.26RNA binding motif protein X-linked [Source:NCBI ge... [more]
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BLAST of Transformer 2 protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
NSR15.979e-931.65Nucleolar protein that binds nuclear localization ... [more]
NAM85.477e-836.76RNA binding protein, component of the U1 snRNP pro... [more]
MIP69.386e-734.72Putative RNA-binding protein; interacts with Mex67... [more]
HRP11.609e-645.65Subunit of cleavage factor I; cleavage factor I is... [more]
NOP152.722e-638.10Constituent of 66S pre-ribosomal particles; involv... [more]
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BLAST of Transformer 2 protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO448021.276e-3854.47Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO448905.004e-1037.35Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO399522.551e-938.96Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO492823.761e-933.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO297158.604e-938.03Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Transformer 2 protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tra2a6.178e-3552.21transformer 2a [Source:NCBI gene;Acc:100049284][more]
tra2a2.947e-3450.00transformer-2 protein homolog alpha [Source:NCBI g... [more]
tra2b1.632e-3357.14transformer 2 beta homolog [Source:NCBI gene;Acc:1... [more]
tra2b2.679e-3357.14transformer 2 beta homolog [Source:NCBI gene;Acc:1... [more]
tra2b6.650e-3357.14transformer 2 beta homolog [Source:NCBI gene;Acc:1... [more]
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BLAST of Transformer 2 protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000022369.15.350e-98100.00SMESG000022369.1[more]
SMESG000031639.17.760e-3757.28SMESG000031639.1[more]
SMESG000031639.18.053e-3757.28SMESG000031639.1[more]
SMESG000031644.11.507e-3657.28SMESG000031644.1[more]
SMESG000031644.11.596e-3657.28SMESG000031644.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012773 ID=SMED30012773|Name=Transformer 2 protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1585bp
GTTTAAAGTATGTTTTATTTGTTATGTTCTTAAATGTTAAAATGGAGATT
AATTATTAACTTTTTCAGTTAAGTTGTTATATTAAAAGACAATTTAATTA
ATTTAGAACTTTTATTTTATAAACCTATTTATTAACTAAGGTTATATTTA
ATCCATTTGAACCTTATAGAAGCAATATTGTTACAATGAGTGATAAAAAA
ATTGACAAAGAAGACAGAGATAATCCTTTACCCTCGAAGTGCTTAGGCAT
TTTTAATTTGTCTCTAGAAACCACTGAAAAAGACTTGCACGAAATGTTTT
TTCGATATGGTGAAATTGAAGATGTAAGCTTGGTTATTGATAAAGTCACA
AAAAGATCAAGGGGATTTGGTTTTGTTTATTTTAAGAATATAGATGATGC
TGAAATAGCAAAAGAAAAAGTTCATGGAACTATAGTTCAAGGCAAACAAA
TAAGAGTGGATTATAGTGTCACTCTACGAGCTCACACTCCAACACCAGGT
GTTTACAAAGGAACTAAAACACATGATATTGGAAGAAAACCTAACAAAAG
TCGATCAAATAGAAGAAATCCTCGTGATAGATTGTCAGATCGTGATCGAA
GTGAACAGCGAATGGATTCTAGGTATAGATCAGGTGAAAGCTTTGATAGG
CGTGGTGGTCGTTATGAATATAGTGAAAGAAGATCTAGAGATTGGACTGA
TTATCCACCAAGACGTAGATCTCCTTTTAATGATAGGCCTCATTATTAAT
CGTTTTTTAAAAATATAATGTACAATTATTTACTAATGTGAACGTTTGAG
ACAGAGTACTGTGTTTCATTGGTTAAAATTCCAATTAATTATGTAAATAA
ATAACCCAATCCGTTTAGTAGAAATAGAATTGTGCAATTCAAAGTAATAT
ATAAGGAATTGTTATTAAGTATTGCTGATTTATTGTTTTTCATTGTTATT
TCTTTTTGTAAAATTTTGCAACGGTTTCAATTAATATATTATTTACTATC
TAATATTTTATTTGTGTTTTGCAGTTTATATTTTATTATAATTTTTTAAC
GATTTTTAAAGTTTTATTTAATCTATTATTTATCTGAATTGTGTTGAAAT
GGCTTTTGTAATTGCTTGATTTGAATTGGAGGTTTTCATCAGTTATGTAT
GCAAATTAATAATTTCAATTGTTTGTTAATGAGTAATAAAATTTCTCTTT
TATTTATATATGGTTTGGTATAACTCTTATGCTATGAATTAAATTTTCAG
GTCTATCAAGTTAGATAAATATCATTTTCTGATTATTATTATGAAACATT
TTCCTTTCATTGTGAGATGGCCAAACTTTATAATGGATAAGCATGTTTTT
CTATACTGATACGTTTTTTTACTGGTAACAAAATATGAAATGAGTGTTAT
TGCATCACATTTAGAATTGTGGTTTGTAAAGTAATTTACATGGATTTTTT
GATTGTATGTATTATTTATGATTTTACGTAGTTGGTATTATTTTACACCA
TGTTATCAAAAATTTTATTGACCAAGAAAAATTATTGTTACATTAATTAA
TAACGAAAATTTGTTAATGAGTAAAATTAAGTTGG
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protein sequence of SMED30012773-orf-1

>SMED30012773-orf-1 ID=SMED30012773-orf-1|Name=SMED30012773-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=188bp
MSDKKIDKEDRDNPLPSKCLGIFNLSLETTEKDLHEMFFRYGEIEDVSLV
IDKVTKRSRGFGFVYFKNIDDAEIAKEKVHGTIVQGKQIRVDYSVTLRAH
TPTPGVYKGTKTHDIGRKPNKSRSNRRNPRDRLSDRDRSEQRMDSRYRSG
ESFDRRGGRYEYSERRSRDWTDYPPRRRSPFNDRPHY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003723RNA binding
GO:0003676nucleic acid binding
GO:0000166nucleotide binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR035979RBD_domain_sf
IPR000504RRM_dom
IPR012677Nucleotide-bd_a/b_plait_sf
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 19..92
e-value: 4.1E-24
score: 96.1
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 22..90
e-value: 1.8E-19
score: 69.3
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 18..96
score: 18.018
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3DG3DSA:3.30.70.330coord: 1..101
e-value: 3.7E-27
score: 97.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 111..187
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 102..187
NoneNo IPR availablePANTHERPTHR15241TRANSFORMER-2-RELATEDcoord: 12..117
NoneNo IPR availablePANTHERPTHR15241:SF3TRANSFORMER-2 PROTEIN HOMOLOG ALPHAcoord: 12..117
NoneNo IPR availableCDDcd12363RRM_TRA2coord: 19..96
e-value: 8.52847E-33
score: 110.407
IPR035979RNA-binding domain superfamilySUPERFAMILYSSF54928RNA-binding domain, RBDcoord: 13..122