Ast2 protein

Overview
NameAst2 protein (CCW36840.1)
Smed IDSMED30012714
Length (bp)864
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012714

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 20

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30012714h1SMcG0009025 SmedASXL_009673SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30012714h1SMcG0009025 SmedASXL_009673SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30012714h1SMcG0009025 SmedASXL_009674SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30012714h1SMcG0009025 SmedASXL_009674SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30012714h1SMcG0009025 SmedASXL_009673SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30012714h1SMcG0009025 SmedASXL_009673SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30012714h1SMcG0009025 SmedASXL_009674SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30012714h1SMcG0009025 SmedASXL_009674SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30012714h1SMcG0009037 Contig4486GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012714h1SMcG0009027 Contig4486GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012714h1SMcG0009025 Contig4486GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012714h1SMcG0009037 Contig4486GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012714h1SMcG0009027 Contig4486GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012714h1SMcG0009025 Contig4486GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30012714h1SMcG0009025 OX_Smed_1.0.21359ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
head regionSMED30012714h1SMcG0009025 OX_Smed_1.0.21359ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
neoblastSMED30012714h1SMcG0009025 SMED_01010_V2_1rGPL14150PMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30012714h1SMcG0009027 SMED_01010_V2_1rGPL14150PMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30012714h1SMcG0009025 SMED_01010_V2_1rGPL14150PMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30012714h1SMcG0009027 SMED_01010_V2_1rGPL14150PMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Ast2 protein vs. Ensembl Human
Match: ASTL (astacin like metalloendopeptidase [Source:HGNC Symbol;Acc:HGNC:31704])

HSP 1 Score: 88.9669 bits (219), Expect = 2.366e-19
Identity = 64/226 (28.32%), Postives = 107/226 (47.35%), Query Frame = 1 
Query:  178 LDNVKMGDMLLDSTDIQIINGFKNHYANSKKWP---NNIVTYTF--AGNFPQDKIQTVRQSLGELQKDLQNCVKF---QEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMY-SKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV-LRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            L+       L++  DI   + F+   A S KWP   + +V   F  +  + +   Q + ++L E ++    C++F   Q+ R+  +I +     GC+S +G +GG  Q ++L  P C+   +G + HE +H LGF H H R DRD +I + W+ IL      F K     +  PY+ +SVMHY    F+      +  L      IG   + + +D+  +   YGC
Sbjct:   64 LEETPESSFLIEG-DIIRPSPFRLLSATSNKWPMGGSGVVEVPFLLSSKYDEPSRQVILEALAEFERS--TCIRFVTYQDQRD--FISII-PMYGCFSSVGRSGG-MQVVSLA-PTCLQKGRGIVLHELMHVLGFWHEHTRADRDRYIRVNWNEILPGFEINFIKSQSSNMLTPYDYSSVMHYGRLAFSRRGLPTITPLWAPSVHIGQRWNLSASDITRVLKLYGC 281
[Close]
BLAST of Ast2 protein vs. Ensembl Human
Match: MEP1B (meprin A subunit beta [Source:HGNC Symbol;Acc:HGNC:7020])

HSP 1 Score: 82.8037 bits (203), Expect = 3.983e-17
Identity = 70/224 (31.25%), Postives = 103/224 (45.98%), Query Frame = 1 
Query:  175 GLDNVKMGDMLLDSTDIQIINGFKNHYANSK-KWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQE-GRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQF-TKCDGC--ELDGPYETTSVMHYNSEGFACDPREKVVLRKDG--GAIGYNHDTTPNDLNMIRNFYGC 825
            GLD  + GD+ LD   I      +N     K +WP+ I  Y    +   +    +  +    +  L+ C+ F+      +YI V  K  GC+S +G      Q L++    C     T++HEF+HALGF H   R DRD+++ I WDRIL+ +   F T  D     L+ PY+ TSVMHY+   F       +V R       IG   D + +DL  +   Y C
Sbjct:   45 GLDLFE-GDIRLDRAQI------RNSIIGEKYRWPHTI-PYVLEDSLEMNAKGVILNAFERYR--LKTCIDFKPWAGETNYISV-FKGSGCWSSVGNRRVGKQELSI-GANCD-RIATVQHEFLHALGFWHEQSRSDRDDYVRIMWDRILSGREHNFNTYSDDISDSLNVPYDYTSVMHYSKTAFQNGTEPTIVTRISDFEDVIGQRMDFSDSDLLKLNQLYNC 255
[Close]
BLAST of Ast2 protein vs. Ensembl Human
Match: MEP1B (meprin A subunit beta [Source:HGNC Symbol;Acc:HGNC:7020])

HSP 1 Score: 82.8037 bits (203), Expect = 4.023e-17
Identity = 70/224 (31.25%), Postives = 103/224 (45.98%), Query Frame = 1 
Query:  175 GLDNVKMGDMLLDSTDIQIINGFKNHYANSK-KWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQE-GRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQF-TKCDGC--ELDGPYETTSVMHYNSEGFACDPREKVVLRKDG--GAIGYNHDTTPNDLNMIRNFYGC 825
            GLD  + GD+ LD   I      +N     K +WP+ I  Y    +   +    +  +    +  L+ C+ F+      +YI V  K  GC+S +G      Q L++    C     T++HEF+HALGF H   R DRD+++ I WDRIL+ +   F T  D     L+ PY+ TSVMHY+   F       +V R       IG   D + +DL  +   Y C
Sbjct:   45 GLDLFE-GDIRLDRAQI------RNSIIGEKYRWPHTI-PYVLEDSLEMNAKGVILNAFERYR--LKTCIDFKPWAGETNYISV-FKGSGCWSSVGNRRVGKQELSI-GANCD-RIATVQHEFLHALGFWHEQSRSDRDDYVRIMWDRILSGREHNFNTYSDDISDSLNVPYDYTSVMHYSKTAFQNGTEPTIVTRISDFEDVIGQRMDFSDSDLLKLNQLYNC 255
[Close]
BLAST of Ast2 protein vs. Ensembl Human
Match: TLL1 (tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843])

HSP 1 Score: 78.5666 bits (192), Expect = 7.520e-16
Identity = 58/196 (29.59%), Postives = 86/196 (43.88%), Query Frame = 1 
Query:  265 KKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGH-YIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDG---PYETTSVMHYNSEGFACDPREKVVL--RKDGG---AIGYNHDTTPNDLNMIRNFYGC 825
            + WP  ++ Y   GNF   +    +Q++   +K    CV F E  +   YI    +  GC S +G  G   Q +++      +  G + HE  H +GF H H R DRDNH+TI  + I   Q   F K +  E++     Y+  S+MHY    F+       +L  R D G   AIG     +  D+   R  Y C
Sbjct:  155 RIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEK--HTCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKF--GIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRC 346
[Close]
BLAST of Ast2 protein vs. Ensembl Human
Match: TLL1 (tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843])

HSP 1 Score: 77.7962 bits (190), Expect = 2.316e-15
Identity = 58/194 (29.90%), Postives = 85/194 (43.81%), Query Frame = 1 
Query:  271 WPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGH-YIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDG---PYETTSVMHYNSEGFACDPREKVVL--RKDGG---AIGYNHDTTPNDLNMIRNFYGC 825
            WP  ++ Y   GNF   +    +Q++   +K    CV F E  +   YI    +  GC S +G  G   Q +++      +  G + HE  H +GF H H R DRDNH+TI  + I   Q   F K +  E++     Y+  S+MHY    F+       +L  R D G   AIG     +  D+   R  Y C
Sbjct:  157 WPGGVIPYVIGGNFTGSQRAMFKQAMRHWEK--HTCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKF--GIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRC 346
[Close]
BLAST of Ast2 protein vs. Ensembl Celegans
Match: nas-4 (Zinc metalloproteinase nas-4 [Source:UniProtKB/Swiss-Prot;Acc:P55112])

HSP 1 Score: 105.916 bits (263), Expect = 2.074e-26
Identity = 71/230 (30.87%), Postives = 115/230 (50.00%), Query Frame = 1 
Query:  178 LDNVKMGDMLLDSTDIQI-------INGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKF---QEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDG---CELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 825
            + N   GD+LL+S    +        N  K  Y   ++WPNN + YT +  +       +  ++ E     + CVKF      ++  Y+ ++   EGCYS++G TGG  QP++L++ GC+   GTI HE +HA+GF H   R+DRD++I + W  ++     QF K +      LD PY+  S+MHY    F+   ++ +V +K G   +G     +  D+  I   Y C
Sbjct:   69 IGNYSEGDILLESPKKFVEENNKLGRNAIKQIY---RRWPNNEIPYTLSSQYGSYARSVIANAMNEYHT--KTCVKFVARDPSKHHDYLWIHPD-EGCYSLVGKTGG-KQPVSLDS-GCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFSGSGKKTLVPKKSGSERMGQRVKFSDIDVRKINKLYNC 289
[Close]
BLAST of Ast2 protein vs. Ensembl Celegans
Match: nas-4 (Zinc metalloproteinase nas-4 [Source:UniProtKB/Swiss-Prot;Acc:P55112])

HSP 1 Score: 105.531 bits (262), Expect = 5.294e-26
Identity = 71/230 (30.87%), Postives = 115/230 (50.00%), Query Frame = 1 
Query:  178 LDNVKMGDMLLDSTDIQI-------INGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKF---QEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDG---CELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 825
            + N   GD+LL+S    +        N  K  Y   ++WPNN + YT +  +       +  ++ E     + CVKF      ++  Y+ ++   EGCYS++G TGG  QP++L++ GC+   GTI HE +HA+GF H   R+DRD++I + W  ++     QF K +      LD PY+  S+MHY    F+   ++ +V +K G   +G     +  D+  I   Y C
Sbjct:   69 IGNYSEGDILLESPKKFVEENNKLGRNAIKQIY---RRWPNNEIPYTLSSQYGSYARSVIANAMNEYHT--KTCVKFVARDPSKHHDYLWIHPD-EGCYSLVGKTGG-KQPVSLDS-GCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFSGSGKKTLVPKKSGSERMGQRVKFSDIDVRKINKLYNC 289
[Close]
BLAST of Ast2 protein vs. Ensembl Celegans
Match: nas-4 (Zinc metalloproteinase nas-4 [Source:UniProtKB/Swiss-Prot;Acc:P55112])

HSP 1 Score: 105.145 bits (261), Expect = 5.368e-26
Identity = 71/230 (30.87%), Postives = 115/230 (50.00%), Query Frame = 1 
Query:  178 LDNVKMGDMLLDSTDIQI-------INGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKF---QEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDG---CELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 825
            + N   GD+LL+S    +        N  K  Y   ++WPNN + YT +  +       +  ++ E     + CVKF      ++  Y+ ++   EGCYS++G TGG  QP++L++ GC+   GTI HE +HA+GF H   R+DRD++I + W  ++     QF K +      LD PY+  S+MHY    F+   ++ +V +K G   +G     +  D+  I   Y C
Sbjct:   69 IGNYSEGDILLESPKKFVEENNKLGRNAIKQIY---RRWPNNEIPYTLSSQYGSYARSVIANAMNEYHT--KTCVKFVARDPSKHHDYLWIHPD-EGCYSLVGKTGG-KQPVSLDS-GCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFSGSGKKTLVPKKSGSERMGQRVKFSDIDVRKINKLYNC 289
[Close]
BLAST of Ast2 protein vs. Ensembl Celegans
Match: nas-6 (Zinc metalloproteinase nas-6 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3S9])

HSP 1 Score: 99.7525 bits (247), Expect = 5.936e-24
Identity = 68/205 (33.17%), Postives = 102/205 (49.76%), Query Frame = 1 
Query:  229 IINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVN-SKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGC--ELDGP-YETTSVMHYNSEGFACDPREKVVLRKDG--GAIGYNHDTTPNDLNMIRNFYGC 825
            + N  KN       W   ++ Y     F  ++I+ + ++    ++    C++F E R G    +N  K  GCYS +G TGG  Q ++L   GC + +  I HE +H++GF H H R DRD+HI I WD IL    SQF K      +L G  Y+  S+MHY+S  F+ + R  +   ++G    IG   D +P D+  I   Y C
Sbjct:   70 LFNALKN---KQLTWEGGVIPYEMDTAFSPNEIKILEKAFDSYRR--TTCIRF-EKREGQTDYLNIVKGYGCYSQVGRTGG-KQEISLGR-GCFFHE-IIVHELMHSVGFWHEHSRADRDDHIKINWDNILPGMKSQFDKISAVLQDLQGENYDYKSIMHYDSTAFSRNGRNTIETVENGFTQVIGTAMDLSPLDIVKINKLYSC 265
[Close]
BLAST of Ast2 protein vs. Ensembl Celegans
Match: nas-15 (Zinc metalloproteinase nas-15 [Source:UniProtKB/Swiss-Prot;Acc:P55115])

HSP 1 Score: 95.9005 bits (237), Expect = 4.986e-22
Identity = 67/209 (32.06%), Postives = 103/209 (49.28%), Query Frame = 1 
Query:  214 STDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKF--QEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGC---ELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            S D +  N  KN     + WP   + YT +  +       +  S+ E       C+++  +E  + +Y+ +   R GCYSM+G  GG  Q L+L + GC+  KG I HE +HA+GF H   R DRD+HITI W+ I      QF K        L   Y+  S+MHY ++ F+ + +  ++ +K+G  IG  +  +  D   I   YGC
Sbjct:  107 SEDGKWYNAIKNRL---QLWPEGRIPYTISSQYSSYSRSLIAASMQEYAS--HTCIRWVPKEAADVNYVHIYPDR-GCYSMVGKMGG-KQSLSLGS-GCI-QKGIILHELMHAVGFFHEQSRTDRDDHITIMWNNIQAGMQGQFEKYGHGTIQSLGTGYDYGSIMHYGTKAFSRNGQPTMIPKKNGATIGQRNGFSKVDKFKINTLYGC 306
[Close]
BLAST of Ast2 protein vs. Ensembl Fly
Match: CG6763 (gene:FBgn0039069 transcript:FBtr0084378)

HSP 1 Score: 129.798 bits (325), Expect = 6.003e-35
Identity = 69/214 (32.24%), Postives = 114/214 (53.27%), Query Frame = 1 
Query:  196 GDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKF-QEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDG-PYETTSVMHYNSEGFACDPREKVVLRKDGGA--IGYNHDTTPNDLNMIRNFYGC 825
            GDML+  TD+ + NG       S +WPN +V Y   GNF    + T+  ++GE  +  + C++F +      YI +     GC+S +G  GG  Q +NL++PGC+   GT  HE +HALGF+H   R +RD ++ I+++ + +S  + F K    E  G PY+  SVMHY+   F+ + +  ++  +  GA  +G  +  +  D+  +   Y C
Sbjct:   95 GDMLVPQTDLIMKNGLPTQ---SSRWPNGVVPYEIRGNFNARDMATIENAIGEYHR--RTCIRFVKRSSERDYISIRGDNSGCWSSVGRVGG-KQEVNLQSPGCLSRPGTAMHELMHALGFLHEQNRMERDGYVAIQYNNVQSSAMNNFEKAARTEAFGVPYDYGSVMHYSKNAFSINGQPTILAMQANGADKMGQRNGFSDYDIQKLNRMYDC 302
[Close]
BLAST of Ast2 protein vs. Ensembl Fly
Match: CG6763 (gene:FBgn0039069 transcript:FBtr0334497)

HSP 1 Score: 129.798 bits (325), Expect = 6.003e-35
Identity = 69/214 (32.24%), Postives = 114/214 (53.27%), Query Frame = 1 
Query:  196 GDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKF-QEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDG-PYETTSVMHYNSEGFACDPREKVVLRKDGGA--IGYNHDTTPNDLNMIRNFYGC 825
            GDML+  TD+ + NG       S +WPN +V Y   GNF    + T+  ++GE  +  + C++F +      YI +     GC+S +G  GG  Q +NL++PGC+   GT  HE +HALGF+H   R +RD ++ I+++ + +S  + F K    E  G PY+  SVMHY+   F+ + +  ++  +  GA  +G  +  +  D+  +   Y C
Sbjct:   95 GDMLVPQTDLIMKNGLPTQ---SSRWPNGVVPYEIRGNFNARDMATIENAIGEYHR--RTCIRFVKRSSERDYISIRGDNSGCWSSVGRVGG-KQEVNLQSPGCLSRPGTAMHELMHALGFLHEQNRMERDGYVAIQYNNVQSSAMNNFEKAARTEAFGVPYDYGSVMHYSKNAFSINGQPTILAMQANGADKMGQRNGFSDYDIQKLNRMYDC 302
[Close]
BLAST of Ast2 protein vs. Ensembl Fly
Match: CG11865 (gene:FBgn0028947 transcript:FBtr0080753)

HSP 1 Score: 96.6709 bits (239), Expect = 1.842e-23
Identity = 71/254 (27.95%), Postives = 115/254 (45.28%), Query Frame = 1 
Query:  100 LGFVVIDYANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQ---------IINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQ--EGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQF--TKCDGCELDG-PYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFY 819
            L   VI +  G     R S+KL+          D++L S  +Q         ++  +  +Y     W    + Y++AG F    I ++  ++ E+    + CVKF+  E +    + +  +  GC+S +G  G   Q LNL   GCM S  TI+HE +HALGF HTH    RD ++ I+ D I +     F   + +G    G  Y+  S+MHY    F+ + +  +V  K    IG     +P D+  ++  Y
Sbjct:    4 LALAVI-FGLGSSANGRPSIKLQE--------DDIILISEQLQYFEGNLEGRVVKSWSEYY-----WKGRTLVYSYAGGFSSLDIASIESAMAEISS--KTCVKFRRTEYKREPQVVIQKEGSGCWSYVGYLGRADQTLNL-GSGCM-SNRTIQHELLHALGFFHTHSDPQRDKYVRIQTDNIRSGHEHNFQRLRANGVTNYGFGYDYDSIMHYGPFAFSKNGQSTIVPLKSHAKIGQATQMSPKDVQTLKRMY 239
[Close]
BLAST of Ast2 protein vs. Ensembl Fly
Match: CG15255 (gene:FBgn0028950 transcript:FBtr0080785)

HSP 1 Score: 94.3597 bits (233), Expect = 1.708e-22
Identity = 60/206 (29.13%), Postives = 97/206 (47.09%), Query Frame = 1 
Query:  235 NGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEG--RNGHYIEVNSKREGCYSMLGVTGGPSQPLNLE----NPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDG---CELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            NG  N     K+WP N+V Y  + +F     + ++  +  L+  L  C++F+E    +  Y+ V +K  GCY+ +G  G P Q +NLE      GC +  GTI HEF+HALGF H      RD+ I + ++ I+  +   F K       + +  Y+  S +HY    F+ +  + +V       IG     +  D++ I   Y C
Sbjct:   59 NGLIN---TEKRWPGNVVVYRISDDFDTAHKKAIQTGIDTLE--LHTCLRFREATDEDKAYLTVTAKSGGCYTAVGYQGAP-QEMNLEIYPLGEGC-FRPGTILHEFMHALGFYHQQSSSIRDDFINVIYENIVPGKEFNFQKYADTVVTDFEVGYDYDSCLHYRPGAFSINGEDTIVPLDSSAVIGQRVGLSSKDIDKINIMYKC 257
[Close]
BLAST of Ast2 protein vs. Ensembl Fly
Match: CG15255 (gene:FBgn0028950 transcript:FBtr0343719)

HSP 1 Score: 94.3597 bits (233), Expect = 1.708e-22
Identity = 60/206 (29.13%), Postives = 97/206 (47.09%), Query Frame = 1 
Query:  235 NGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEG--RNGHYIEVNSKREGCYSMLGVTGGPSQPLNLE----NPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDG---CELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            NG  N     K+WP N+V Y  + +F     + ++  +  L+  L  C++F+E    +  Y+ V +K  GCY+ +G  G P Q +NLE      GC +  GTI HEF+HALGF H      RD+ I + ++ I+  +   F K       + +  Y+  S +HY    F+ +  + +V       IG     +  D++ I   Y C
Sbjct:   59 NGLIN---TEKRWPGNVVVYRISDDFDTAHKKAIQTGIDTLE--LHTCLRFREATDEDKAYLTVTAKSGGCYTAVGYQGAP-QEMNLEIYPLGEGC-FRPGTILHEFMHALGFYHQQSSSIRDDFINVIYENIVPGKEFNFQKYADTVVTDFEVGYDYDSCLHYRPGAFSINGEDTIVPLDSSAVIGQRVGLSSKDIDKINIMYKC 257
[Close]
BLAST of Ast2 protein vs. Ensembl Zebrafish
Match: hce2l1 (high choriolytic enzyme 2-like 1 [Source:ZFIN;Acc:ZDB-GENE-070912-147])

HSP 1 Score: 102.449 bits (254), Expect = 9.183e-26
Identity = 62/195 (31.79%), Postives = 99/195 (50.77%), Query Frame = 1 
Query:  256 ANSKKWPNNIVTYTFAGNFPQ---DKIQTVRQSLGELQKDLQNCVKF-QEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGG-AIGYNHDTTPNDLNMIRNFYGC 825
             +S +WP  +  + +         D +  +    G L      CVKF       +++ +   R GC+S LG+TGG SQ ++L++PGCM+S G   HE +HALGF+H   R DRD +++I W+ I+ +Q   F K +   L+  Y+ +SVMHY    F+ D    ++ + D    IG     +  D++ I   Y C
Sbjct:   24 GDSCRWPKAVDGFVYVPYIMSTLYDDMDRITIETGMLDISSSTCVKFVPRTHQANFLNIQ-PRYGCWSYLGMTGG-SQTVSLQSPGCMWS-GVASHELMHALGFVHEQSRSDRDRYVSILWENIIENQRHNFRKYETNNLNTAYDYSSVMHYGRYAFSEDGGPTIIPKPDPYIPIGQRDGPSILDIHKINILYNC 215
[Close]
BLAST of Ast2 protein vs. Ensembl Zebrafish
Match: hce2l1 (high choriolytic enzyme 2-like 1 [Source:ZFIN;Acc:ZDB-GENE-070912-147])

HSP 1 Score: 102.449 bits (254), Expect = 2.877e-25
Identity = 64/197 (32.49%), Postives = 101/197 (51.27%), Query Frame = 1 
Query:  256 ANSKKWPNNI-----VTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKF-QEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGG-AIGYNHDTTPNDLNMIRNFYGC 825
             +S +WP  +     V Y  +  +  D +  +    G L      CVKF       +++ +   R GC+S LG+TGG SQ ++L++PGCM+S G   HE +HALGF+H   R DRD +++I W+ I+ +Q   F K +   L+  Y+ +SVMHY    F+ D    ++ + D    IG     +  D++ I   Y C
Sbjct:   82 GDSCRWPKAVDGFVYVPYIMSTLY--DDMDRITIETGMLDISSSTCVKFVPRTHQANFLNIQ-PRYGCWSYLGMTGG-SQTVSLQSPGCMWS-GVASHELMHALGFVHEQSRSDRDRYVSILWENIIENQRHNFRKYETNNLNTAYDYSSVMHYGRYAFSEDGGPTIIPKPDPYIPIGQRDGPSILDIHKINILYNC 273
[Close]
BLAST of Ast2 protein vs. Ensembl Zebrafish
Match: hce2l2 (high choriolytic enzyme 2-like 2 [Source:ZFIN;Acc:ZDB-GENE-070912-146])

HSP 1 Score: 99.7525 bits (247), Expect = 3.239e-24
Identity = 60/201 (29.85%), Postives = 100/201 (49.75%), Query Frame = 1 
Query:  244 KNHYANSKKWPNNI-----VTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKF-QEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 825
            KN +A S  W  ++     + Y+ +  +    ++ +++ +  +++D   CV+F        Y+++  K  GC+S LG  GG  Q ++L++P C  S G   HE +HALGF+H   R DRD ++TI W  I   +   F K     LD PY+ +SVMH+    F+ D    +V +++    IG     +  D+  I   Y C
Sbjct:   78 KNCFARSCLWTKSVDGNVYIAYSLSHAYDDADVKNIKEGMELIEQD--TCVRFVPRTHQRDYLDIQPK-TGCWSYLGARGG-RQTISLQSPDCTGS-GVTVHELMHALGFVHEQSRADRDKYVTIMWSNIWKDRLRNFEKFKTNNLDTPYDYSSVMHFGKYAFSEDGEPTIVPKRNWNVKIGQRLGPSDLDIMKINKLYSC 273
[Close]
BLAST of Ast2 protein vs. Ensembl Zebrafish
Match: hce2l2 (high choriolytic enzyme 2-like 2 [Source:ZFIN;Acc:ZDB-GENE-070912-146])

HSP 1 Score: 98.2117 bits (243), Expect = 3.303e-24
Identity = 57/195 (29.23%), Postives = 101/195 (51.79%), Query Frame = 1 
Query:  244 KNHYANSKKWPNNI-----VTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKF-QEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIR 810
            KN +A S  W  ++     + Y+ +  +    ++ +++ +  +++D   CV+F        Y+++  K  GC+S LG  GG  Q ++L++P C  S G   HE +HALGF+H   R DRD ++TI W  I   +   F K     LD PY+ +SVMH+    F+ D    +V +++   +       P+DL++++
Sbjct:   19 KNCFARSCLWTKSVDGNVYIAYSLSHAYDDADVKNIKEGMELIEQD--TCVRFVPRTHQRDYLDIQPK-TGCWSYLGARGG-RQTISLQSPDCTGS-GVTVHELMHALGFVHEQSRADRDKYVTIMWSNIWKDRLRNFEKFKTNNLDTPYDYSSVMHFGKYAFSEDGEPTIVPKRNWN-VKIGQRLGPSDLDIMK 207
[Close]
BLAST of Ast2 protein vs. Ensembl Zebrafish
Match: c6ast3 (six-cysteine containing astacin protease 3 [Source:ZFIN;Acc:ZDB-GENE-050320-99])

HSP 1 Score: 98.9821 bits (245), Expect = 3.309e-24
Identity = 57/182 (31.32%), Postives = 92/182 (50.55%), Query Frame = 1 
Query:  286 VTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKF-QEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV-LRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            V Y  A ++   +++ +++ L         C++F   G    YI + S R GCYS +G   G +Q ++L   GC+Y   T++HE +HALGF H   R DRDNHI + W+ IL      F K +      PY+ +SVM Y    F+ +    ++ +  +  A+G + + + ND+  I   Y C
Sbjct:   79 VPYVIANHYSSRELEIIQRGLDSF--SYSTCIRFFPRGNERDYISIES-RSGCYSYVG-RQGYAQTVSLARSGCLY-HSTVQHELLHALGFNHEQTRNDRDNHIQVIWENILDDMKYNFNKINTLNQGTPYDYSSVMQYERYAFSKNGLPTMIPIPNNNAALGTSTEMSQNDIIRINRLYQC 255
[Close]
BLAST of Ast2 protein vs. Ensembl Xenopus
Match: polr2l.2 (polymerase (RNA) II (DNA directed) polypeptide L, 7.6kDa, gene 2 [Source:Xenbase;Acc:XB-GENE-5898446])

HSP 1 Score: 103.219 bits (256), Expect = 2.949e-24
Identity = 69/210 (32.86%), Postives = 106/210 (50.48%), Query Frame = 1 
Query:  220 DIQIINGFKNHYANSKKWPNNI-----VTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQE-GRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFA-CDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 825
            DI I  G       S  WP +      V Y  + ++  ++  ++R +L  L+ +   CV F E      ++++ S   GC+S +G TGG +Q L+L + GC+ +KG I+HE  HALGF H   R DRD +I + W  I  S    F   D   LD PY+ +SVMHY    F+    +  +  + D  A IG  +  +P D++ ++  YGC
Sbjct:   54 DIAIETGRSATKCPSCLWPKSADGTVRVPYRLSADYSDNEKSSIRDAL--LEFNTLTCVHFVERSTEEDFLDIVSD-SGCWSSIGRTGG-AQTLSLMSSGCL-AKGIIQHEVDHALGFYHEQSRSDRDTYIDVLWQYICESDWGSFEMVDTDNLDLPYDYSSVMHYGWYAFSNTSGQPSLRPKPDPTANIGQRYGLSPLDVSKVKELYGC 258
[Close]
BLAST of Ast2 protein vs. Ensembl Xenopus
Match: kpna4 (karyopherin alpha 4 (importin alpha 3) [Source:Xenbase;Acc:XB-GENE-1011058])

HSP 1 Score: 98.5969 bits (244), Expect = 1.138e-22
Identity = 70/231 (30.30%), Postives = 116/231 (50.22%), Query Frame = 1 
Query:  196 GDMLLDSTDIQIINGFKNHYANSKKWPNN-----IVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKF-QEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA--IGYNHDTTPNDLNMIRNFYGC*RR*KTNICKICL 864
            G+M L      +IN  ++  ++   WP +     +V Y F+ N+  D++   + ++ E +     CV+F  +     ++ + S   GC SM+G  GG  Q + L + GCM S+G I+HE  HALGF H HMR DRD+++TI  + I+ S  + F+K     +   Y+  SVMHY+   F+  P +  ++ K   +  IG     +  D+  I+  Y C      ++C I L
Sbjct:   49 GNMALTEGGDILINIGRSATSSDYLWPKSADGTVVVPYIFSYNYSADELTLFKTAMQEFET--LTCVRFVPKTIQRDFLNIVSNG-GCLSMVGRNGG-GQKVELASYGCM-SRGVIQHELNHALGFYHEHMRSDRDDYVTIITENIIPSYENYFSKRKTNNMGIIYDYNSVMHYSRNTFSISPDKSTIVPKPDPSIPIGQRDGLSILDILKIKKLYQC------DVCAILL 268
[Close]
BLAST of Ast2 protein vs. Ensembl Xenopus
Match: ENSXETT00000032332.1 (astacin-like metallo-endopeptidase 3 gene b1 [Source:NCBI gene;Acc:100498420])

HSP 1 Score: 95.5153 bits (236), Expect = 1.450e-21
Identity = 61/191 (31.94%), Postives = 93/191 (48.69%), Query Frame = 1 
Query:  271 WPNN-----IVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 825
            WP +     IV Y F+ N+  D++   + ++ E +     CV+F    N           GC S LG  GG  Q + L++ GC+Y +G I+HE  HALGF H   R DRD+++TI  + I+      F K D   L   Y+ +SVMHY+ + F+ +    +V + D    IG     +  D++ I   Y C
Sbjct:   51 WPKSTDGTVIVPYNFSSNYSADQLALFKSTMQEYES--LTCVRFVPRANETDFLSIVSDNGCASFLGKVGG-DQTVQLDSYGCIY-RGIIQHELNHALGFYHEQSRSDRDDYVTIHTENIIPGYEGNFNKADSNNLGLEYDYSSVMHYSGDAFSKNGNLTIVPKPDPTVPIGQRDGLSILDVSKINRLYQC 237
[Close]
BLAST of Ast2 protein vs. Ensembl Xenopus
Match: ENSXETT00000032191.1 (astacin-like metallo-endopeptidase 3 gene b1 [Source:NCBI gene;Acc:100498420])

HSP 1 Score: 95.5153 bits (236), Expect = 1.748e-21
Identity = 61/191 (31.94%), Postives = 93/191 (48.69%), Query Frame = 1 
Query:  271 WPNN-----IVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 825
            WP +     IV Y F+ N+  D++   + ++ E +     CV+F    N           GC S LG  GG  Q + L++ GC+Y +G I+HE  HALGF H   R DRD+++TI  + I+      F K D   L   Y+ +SVMHY+ + F+ +    +V + D    IG     +  D++ I   Y C
Sbjct:  124 WPKSTDGTVIVPYNFSSNYSADQLALFKSTMQEYES--LTCVRFVPRANETDFLSIVSDNGCASFLGKVGG-DQTVQLDSYGCIY-RGIIQHELNHALGFYHEQSRSDRDDYVTIHTENIIPGYEGNFNKADSNNLGLEYDYSSVMHYSGDAFSKNGNLTIVPKPDPTVPIGQRDGLSILDVSKINRLYQC 310
[Close]
BLAST of Ast2 protein vs. Ensembl Xenopus
Match: ENSXETT00000032173.1 (astacin-like metallo-endopeptidase 3 gene b1 [Source:NCBI gene;Acc:100498420])

HSP 1 Score: 95.1301 bits (235), Expect = 1.868e-21
Identity = 61/191 (31.94%), Postives = 93/191 (48.69%), Query Frame = 1 
Query:  271 WPNN-----IVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 825
            WP +     IV Y F+ N+  D++   + ++ E +     CV+F    N           GC S LG  GG  Q + L++ GC+Y +G I+HE  HALGF H   R DRD+++TI  + I+      F K D   L   Y+ +SVMHY+ + F+ +    +V + D    IG     +  D++ I   Y C
Sbjct:  124 WPKSTDGTVIVPYNFSSNYSADQLALFKSTMQEYES--LTCVRFVPRANETDFLSIVSDNGCASFLGKVGG-DQTVQLDSYGCIY-RGIIQHELNHALGFYHEQSRSDRDDYVTIHTENIIPGYEGNFNKADSNNLGLEYDYSSVMHYSGDAFSKNGNLTIVPKPDPTVPIGQRDGLSILDVSKINRLYQC 310
[Close]
BLAST of Ast2 protein vs. Ensembl Mouse
Match: Mep1b (meprin 1 beta [Source:MGI Symbol;Acc:MGI:96964])

HSP 1 Score: 82.0333 bits (201), Expect = 5.270e-17
Identity = 66/223 (29.60%), Postives = 102/223 (45.74%), Query Frame = 1 
Query:  175 GLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGR-NGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGC---ELDGPYETTSVMHYNSEGFACDPREKVVLR--KDGGAIGYNHDTTPNDLNMIRNFYGC 825
            GLD  + GD+ L++      NG  +   + K+WP+ I  Y    +   +    +  +    +  L+ C+ F+      +YI V  K  GC+S +G      Q L++    C     T++HEF+HALGF H   R DRD+++ I WDRI   +   F   +      L+ PY+ TSVMHY+   F       +V R  +    IG   D +  DL  +   Y C
Sbjct:   47 GLDLFE-GDIKLEA------NGKNSIIGDHKRWPHTI-PYVLEDSLEMNAKGVILNAFERYR--LKTCIDFKPWSGEANYISV-FKGSGCWSSVGNIHAGKQELSI-GTNCD-RIATVQHEFLHALGFWHEQSRADRDDYVIIVWDRIQPGKEHNFNIYNDSVSDSLNVPYDYTSVMHYSKTAFQNGTESTIVTRISEFEDVIGQRMDFSDYDLLKLNQLYNC 256
[Close]
BLAST of Ast2 protein vs. Ensembl Mouse
Match: Astl (astacin-like metalloendopeptidase (M12 family) [Source:MGI Symbol;Acc:MGI:3046414])

HSP 1 Score: 78.9518 bits (193), Expect = 4.230e-16
Identity = 54/216 (25.00%), Postives = 94/216 (43.52%), Query Frame = 1 
Query:  205 LLDSTDIQIINGFKNHYANSKKWPNNI-----VTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMY-SKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV-LRKDGGAIGYNHDTTPNDLNMIRNFYGC*R 831
             L   DI   + F+     + KWP  +     + +  +  + +   + +  +  E ++    C++F                GC+S +G +GG  Q ++L  P C+   +G + HE +H LGF H H R DRD +I + W+ IL      F K     +  PY+ +SVMHY    F+   +  ++ L      IG   + + +D+  +   Y C R
Sbjct:   72 FLVEGDIIRPSPFRLLSVTNNKWPKGVGGFVEIPFLLSRKYDELSRRVIMDAFAEFERF--TCIRFVAYHGQRDFVSILPMAGCFSGVGRSGG-MQVVSLA-PTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAFSWRGQPTIIPLWTSSVHIGQRWNLSTSDITRVCRLYNCSR 283
[Close]
BLAST of Ast2 protein vs. Ensembl Mouse
Match: Astl (astacin-like metalloendopeptidase (M12 family) [Source:MGI Symbol;Acc:MGI:3046414])

HSP 1 Score: 78.5666 bits (192), Expect = 5.168e-16
Identity = 54/216 (25.00%), Postives = 94/216 (43.52%), Query Frame = 1 
Query:  205 LLDSTDIQIINGFKNHYANSKKWPNNI-----VTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYS-KGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV-LRKDGGAIGYNHDTTPNDLNMIRNFYGC*R 831
             L   DI   + F+     + KWP  +     + +  +  + +   + +  +  E ++    C++F                GC+S +G +GG  Q ++L  P C+   +G + HE +H LGF H H R DRD +I + W+ IL      F K     +  PY+ +SVMHY    F+   +  ++ L      IG   + + +D+  +   Y C R
Sbjct:   51 FLVEGDIIRPSPFRLLSVTNNKWPKGVGGFVEIPFLLSRKYDELSRRVIMDAFAEFERF--TCIRFVAYHGQRDFVSILPMAGCFSGVGRSGG-MQVVSLA-PTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAFSWRGQPTIIPLWTSSVHIGQRWNLSTSDITRVCRLYNCSR 262
[Close]
BLAST of Ast2 protein vs. Ensembl Mouse
Match: Astl (astacin-like metalloendopeptidase (M12 family) [Source:MGI Symbol;Acc:MGI:3046414])

HSP 1 Score: 78.5666 bits (192), Expect = 5.168e-16
Identity = 54/216 (25.00%), Postives = 94/216 (43.52%), Query Frame = 1 
Query:  205 LLDSTDIQIINGFKNHYANSKKWPNNI-----VTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYS-KGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV-LRKDGGAIGYNHDTTPNDLNMIRNFYGC*R 831
             L   DI   + F+     + KWP  +     + +  +  + +   + +  +  E ++    C++F                GC+S +G +GG  Q ++L  P C+   +G + HE +H LGF H H R DRD +I + W+ IL      F K     +  PY+ +SVMHY    F+   +  ++ L      IG   + + +D+  +   Y C R
Sbjct:   51 FLVEGDIIRPSPFRLLSVTNNKWPKGVGGFVEIPFLLSRKYDELSRRVIMDAFAEFERF--TCIRFVAYHGQRDFVSILPMAGCFSGVGRSGG-MQVVSLA-PTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAFSWRGQPTIIPLWTSSVHIGQRWNLSTSDITRVCRLYNCSR 262
[Close]
BLAST of Ast2 protein vs. Ensembl Mouse
Match: Tll2 (tolloid-like 2 [Source:MGI Symbol;Acc:MGI:1346044])

HSP 1 Score: 78.5666 bits (192), Expect = 8.719e-16
Identity = 66/243 (27.16%), Postives = 98/243 (40.33%), Query Frame = 1 
Query:  124 ANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKRE-GCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCE---LDGPYETTSVMHYNSEGFACDPREKVVL--RKDGGA---IGYNHDTTPNDLNMIRNFYGC 825
            +N     PR+  K  T  L N    +    +   ++     +     + WP  ++ Y   GNF   +    +Q++   +K    CV F E  +     V S R  GC S +G  GG  Q +++      +  G + HE  H +GF H H R DRD H+TI  + I   Q   F K +  E   L   Y+  S+MHY    F+       +L  R D G    IG     +  D+   R  Y C
Sbjct:  109 SNASIQAPRKDGKDATTFLPNPGTSNTTAKTFSARVRRATTSR--TERIWPGGVIPYVIGGNFTGTQRAIFKQAMRHWEK--HTCVTFVERTDEESFIVFSYRTCGCCSYVGRRGGGPQAISIGKNCDKF--GIVAHELGHVVGFWHEHTRPDRDQHVTIIRENIQPGQEYNFLKMEAGEVSSLGETYDFDSIMHYARNTFSRGVFLDTILPRRDDNGVRPTIGQRVRLSQGDIAQARKLYKC 345
[Close]
BLAST of Ast2 protein vs. UniProt/SwissProt
Match: sp|P55112|NAS4_CAEEL (Zinc metalloproteinase nas-4 OS=Caenorhabditis elegans OX=6239 GN=nas-4 PE=2 SV=4)

HSP 1 Score: 105.916 bits (263), Expect = 2.638e-25
Identity = 71/230 (30.87%), Postives = 115/230 (50.00%), Query Frame = 1 
Query:  178 LDNVKMGDMLLDSTDIQI-------INGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKF---QEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDG---CELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 825
            + N   GD+LL+S    +        N  K  Y   ++WPNN + YT +  +       +  ++ E     + CVKF      ++  Y+ ++   EGCYS++G TGG  QP++L++ GC+   GTI HE +HA+GF H   R+DRD++I + W  ++     QF K +      LD PY+  S+MHY    F+   ++ +V +K G   +G     +  D+  I   Y C
Sbjct:   69 IGNYSEGDILLESPKKFVEENNKLGRNAIKQIY---RRWPNNEIPYTLSSQYGSYARSVIANAMNEYHT--KTCVKFVARDPSKHHDYLWIHPD-EGCYSLVGKTGG-KQPVSLDS-GCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFSGSGKKTLVPKKSGSERMGQRVKFSDIDVRKINKLYNC 289
[Close]
BLAST of Ast2 protein vs. UniProt/SwissProt
Match: sp|P31579|LCE_ORYLA (Low choriolytic enzyme OS=Oryzias latipes OX=8090 GN=lce PE=1 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 7.564e-25
Identity = 70/229 (30.57%), Postives = 106/229 (46.29%), Query Frame = 1 
Query:  160 KLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYA-NSKKWP---NNIVT--YTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV-LRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            ++    ++ +  GD++L  T     N  K   A +S +WP   N IV   Y  + N+  D+ +T+R ++ E  +  + C+ F    N         R GC SM+G  G   Q + L+  GC+     I+HE +HALGF H H R DRD H+ I W+ I+      F K D   L  PY+  S+MHY    F  D +E +  +     AIG     +  D+  +   Y C
Sbjct:   51 RMNNNSMEELLEGDLVLPKTR----NAMKCFGAPDSCRWPKSSNGIVKVPYVVSDNYESDEKETIRNAMKEFAE--KTCIHFVPRNNERAYLSLEPRFGCKSMMGYVGD-KQVVVLQRFGCI-KHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLGTPYDYGSIMHYGRTAFGKDRKETITPIPNPKAAIGQTERMSDIDILRVNKLYKC 271
[Close]
BLAST of Ast2 protein vs. UniProt/SwissProt
Match: sp|P31581|HCE2_ORYLA (High choriolytic enzyme 2 OS=Oryzias latipes OX=8090 GN=hceb PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 3.075e-23
Identity = 53/146 (36.30%), Postives = 81/146 (55.48%), Query Frame = 1 
Query:  283 IVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGH-YIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFA 717
            ++ Y  +  + + ++ T+  ++       + C++F    N + +I V SK  GCYS LG  GG  Q L+L   GCMYS G I+HE  HALGF H   R DRD+++ I W  I+ +    F K D   L+ PY+ +S+MHY  + F+
Sbjct:  100 VIPYVISSQYSRGEVATIEGAMRAFNG--RTCIRFVRRTNEYDFISVVSK-NGCYSELGRKGG-QQELSLNRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWQNIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFS 240
[Close]
BLAST of Ast2 protein vs. UniProt/SwissProt
Match: sp|Q9U3S9|NAS6_CAEEL (Zinc metalloproteinase nas-6 OS=Caenorhabditis elegans OX=6239 GN=nas-6 PE=2 SV=2)

HSP 1 Score: 99.7525 bits (247), Expect = 7.550e-23
Identity = 68/205 (33.17%), Postives = 102/205 (49.76%), Query Frame = 1 
Query:  229 IINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVN-SKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGC--ELDGP-YETTSVMHYNSEGFACDPREKVVLRKDG--GAIGYNHDTTPNDLNMIRNFYGC 825
            + N  KN       W   ++ Y     F  ++I+ + ++    ++    C++F E R G    +N  K  GCYS +G TGG  Q ++L   GC + +  I HE +H++GF H H R DRD+HI I WD IL    SQF K      +L G  Y+  S+MHY+S  F+ + R  +   ++G    IG   D +P D+  I   Y C
Sbjct:   70 LFNALKN---KQLTWEGGVIPYEMDTAFSPNEIKILEKAFDSYRR--TTCIRF-EKREGQTDYLNIVKGYGCYSQVGRTGG-KQEISLGR-GCFFHE-IIVHELMHSVGFWHEHSRADRDDHIKINWDNILPGMKSQFDKISAVLQDLQGENYDYKSIMHYDSTAFSRNGRNTIETVENGFTQVIGTAMDLSPLDIVKINKLYSC 265
[Close]
BLAST of Ast2 protein vs. UniProt/SwissProt
Match: sp|P31580|HCE1_ORYLA (High choriolytic enzyme 1 OS=Oryzias latipes OX=8090 GN=hcea PE=1 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 1.168e-22
Identity = 52/146 (35.62%), Postives = 81/146 (55.48%), Query Frame = 1 
Query:  283 IVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGH-YIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFA 717
            ++ Y  +  +   ++ T+  ++       + C++F    N + +I V SK  GCYS LG  GG  Q L++   GCMYS G I+HE  HALGF H   R DRD+++ I W+ I+ +    F K D   L+ PY+ +S+MHY  + F+
Sbjct:   91 VIPYVISSEYSGGEVATIEGAMRAFNG--KTCIRFVRRTNEYDFISVVSK-TGCYSELGRKGG-QQELSINRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWENIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFS 231
[Close]
BLAST of Ast2 protein vs. TrEMBL
Match: A0A060Q714 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast2 PE=2 SV=1)

HSP 1 Score: 504.597 bits (1298), Expect = 9.353e-180
Identity = 238/250 (95.20%), Postives = 244/250 (97.60%), Query Frame = 1 
Query:   76 MEKLILLLLGFVVIDYANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            MEKLILLLLGFVVIDYANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCE DGPYET SVMHYNSEGFAC+P EKVV++K+GGAIGYNH+TT NDL MI+NFY C
Sbjct:    1 MEKLILLLLGFVVIDYANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQMIKNFYNC 250
[Close]
BLAST of Ast2 protein vs. TrEMBL
Match: A0A060Q785 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast1 PE=2 SV=1)

HSP 1 Score: 433.335 bits (1113), Expect = 1.214e-151
Identity = 203/251 (80.88%), Postives = 220/251 (87.65%), Query Frame = 1 
Query:   76 MEKLILLLLGFVVIDYANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGG-PSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            M+K+ L+ L   VI Y +GQCGCPRQ  KL  R +D++KMGDMLLD +DI +INGFKNHYAN+KKWPNN V Y+FAGNFP+DKI  VRQ LGELQ+DL NCVKF E   GHYIEVNSK+EGCYS+LG   G P+QPLNLENPGCMYSKGT+KHEFIHALGFMHTHMRKDRDNHITI W RI TSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC
Sbjct:    1 MQKMFLMCLAIAVIGYVSGQCGCPRQ--KLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 249
[Close]
BLAST of Ast2 protein vs. TrEMBL
Match: A0A060Q786 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast6 PE=2 SV=1)

HSP 1 Score: 371.318 bits (952), Expect = 3.947e-127
Identity = 172/250 (68.80%), Postives = 199/250 (79.60%), Query Frame = 1 
Query:   76 MEKLILLLLGFVVIDYANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            M  L  LLL    + Y +GQCGCPRQ  KL  RGLD+++MGDM+LD +DI +INGFK HY+N+K+WPNN V ++FA  FP DK   VR+ L ELQKDL NCVKF E    HYIEV+S  +GCYS+LG TGGP+QPLNL+NPGCMYSKGT+KHEFIHALGFMHTHMRKDRDNHITIKWDRILTS CSQF KC+GC+LDGPYET SVMHY S GFAC P E VV ++DGG I YNH T+ NDL+M+R FY C
Sbjct:    1 MRSLFCLLLIVAAVSYVSGQCGCPRQ--KLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGGPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRKFYAC 248
[Close]
BLAST of Ast2 protein vs. TrEMBL
Match: I1ZIC3 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast3 PE=2 SV=1)

HSP 1 Score: 282.337 bits (721), Expect = 4.797e-92
Identity = 133/250 (53.20%), Postives = 175/250 (70.00%), Query Frame = 1 
Query:   76 MEKLILLLLGFVVIDYANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            M+K ++L++  V+ +    QCGC ++  KL TR  +N+ +GDMLL   DI IINGFK+H   ++ WP   V++ FA +FP DK   V+Q L ELQ DL NCV+F E   G +I V+++ +GCY+M+G TG   Q LNL+NP CM SKGTIKHEFIHALGFMHTHMR DRDN I+I W  I +SQC  F KC+GC +DG YET SVMHY+S+ F C      +++K+G  I YNH+TTPNDL+MI++ Y C
Sbjct:    5 MQKFLILIV-LVLANEVASQCGCNKK--KLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNC 251
[Close]
BLAST of Ast2 protein vs. TrEMBL
Match: A0A060Q750 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast5 PE=2 SV=1)

HSP 1 Score: 229.18 bits (583), Expect = 3.828e-71
Identity = 116/253 (45.85%), Postives = 157/253 (62.06%), Query Frame = 1 
Query:   73 FMEKLILLLLGFVVIDYANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTG--GPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            F+   +L +L  V I+   G+CGC +  +K   R + ++K+ DMLLD  D+  I GF+ +  N+++W +N + YTF   FP DK Q VR+S+ EL  DL+ CVK+ E  +G++I+V S + GC+S +G+    G SQ LNLENPGC+YSKGTIKHEFIHA GFMHT MR DRD  + I W+RI +  C  F  C  CE    Y+T SVMHY SE F C  +   ++ K    I YNH T   D  MI++ Y C
Sbjct:    5 FIGLFLLSILNLVFIE--AGKCGCQKSKLK---RAI-SLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKYTFHPQFPADKKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQC 251
[Close]
BLAST of Ast2 protein vs. Ensembl Cavefish
Match: hce2l1 (high choriolytic enzyme 1-like [Source:NCBI gene;Acc:103038207])

HSP 1 Score: 111.694 bits (278), Expect = 1.036e-28
Identity = 64/190 (33.68%), Postives = 100/190 (52.63%), Query Frame = 1 
Query:  271 WPNNI---VTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKF-QEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGG-AIGYNHDTTPNDLNMIRNFYGC 825
            WP ++   V   +A +   D +  +   +G L    ++CVKF       +++++  K  GC+S LG+ GG +Q L+L+ PGCM+S G   HE +HALGF+H   R DRD ++TI WD I+  Q   F   D   L+  Y+ +SVMHY    F+ +    ++ + D    IG     +P D++ I   Y C
Sbjct:   90 WPKSVDGFVYVPYALSMQYDTMDRITIEMGMLDISSKSCVKFVPRTHQANFLDIQPKF-GCWSYLGMVGG-TQALSLQTPGCMWS-GVASHELMHALGFVHEQSRSDRDRYVTILWDNIIEGQKHNFRMYDTNNLNTAYDYSSVMHYGRFAFSEEGLPTIIPKPDPNIPIGQREGPSPLDIHKINLLYNC 276
[Close]
BLAST of Ast2 protein vs. Ensembl Cavefish
Match: c6ast1 (low choriolytic enzyme-like [Source:NCBI gene;Acc:111191947])

HSP 1 Score: 109.383 bits (272), Expect = 5.548e-28
Identity = 78/228 (34.21%), Postives = 116/228 (50.88%), Query Frame = 1 
Query:  184 NVKMGDMLLDST----DIQIINGFKN-HYANSKK----WP-----NNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQ-EGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV-LRKDGGAIGYNHDTTPNDLNMIRNFY 819
            N  +G  L D T    DI + +G +N     S+K    WP     N  + Y  +  F  ++   +   L   +K    C++F+   R   YI+V S  EGCYS +G TGG  Q L+L++ GC+Y K  I+HEF+HALGF H   R DRD HI I ++ I   +   F K D   L+ PY+  SVMHY  + F+ + +  +V +     A+G   + + ND+  I+  Y
Sbjct:   43 NRNIGQTLEDPTIRFGDILVDDGLQNADRCTSRKPSCMWPMNSDGNTYIPYIISNQFSPNEKDLITSVLKTFEK--STCIRFKPRDREEDYIDVQS-LEGCYSFVGRTGG-RQTLSLKSRGCLYKK-IIQHEFLHALGFHHEQCRSDRDKHIRILYENIDPGKEHNFDKKDTNNLNTPYDYGSVMHYGRDAFSRNGQPTMVPIPNQNVAVGRAEEMSANDILRIKKLY 265
[Close]
BLAST of Ast2 protein vs. Ensembl Cavefish
Match: c6ast1 (low choriolytic enzyme-like [Source:NCBI gene;Acc:111191947])

HSP 1 Score: 109.383 bits (272), Expect = 7.027e-28
Identity = 78/228 (34.21%), Postives = 116/228 (50.88%), Query Frame = 1 
Query:  184 NVKMGDMLLDST----DIQIINGFKN-HYANSKK----WP-----NNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQ-EGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV-LRKDGGAIGYNHDTTPNDLNMIRNFY 819
            N  +G  L D T    DI + +G +N     S+K    WP     N  + Y  +  F  ++   +   L   +K    C++F+   R   YI+V S  EGCYS +G TGG  Q L+L++ GC+Y K  I+HEF+HALGF H   R DRD HI I ++ I   +   F K D   L+ PY+  SVMHY  + F+ + +  +V +     A+G   + + ND+  I+  Y
Sbjct:   54 NRNIGQTLEDPTIRFGDILVDDGLQNADRCTSRKPSCMWPMNSDGNTYIPYIISNQFSPNEKDLITSVLKTFEK--STCIRFKPRDREEDYIDVQS-LEGCYSFVGRTGG-RQTLSLKSRGCLYKK-IIQHEFLHALGFHHEQCRSDRDKHIRILYENIDPGKEHNFDKKDTNNLNTPYDYGSVMHYGRDAFSRNGQPTMVPIPNQNVAVGRAEEMSANDILRIKKLY 276
[Close]
BLAST of Ast2 protein vs. Ensembl Cavefish
Match: npsn (low choriolytic enzyme-like [Source:NCBI gene;Acc:111191946])

HSP 1 Score: 106.301 bits (264), Expect = 1.063e-26
Identity = 74/223 (33.18%), Postives = 112/223 (50.22%), Query Frame = 1 
Query:  187 VKMGDMLLDSTDIQIINGFKN-HYANSKK----WP-----NNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQ-EGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGG-AIGYNHDTTPNDLNMIRNFY 819
            ++ GD+L+D       +G +N     S+K    WP     N  + Y  +  F   +   +   L   +K    C++F+   R   YI+V S  EGCYS +G TGG  Q L+L   GC++ K  I+HE +HALGF H   R DRD HI I ++ I+  +   F K D   L+ PY+  SVMHY  + F+ + +  +V   D   A+G   + + ND+  I+  Y
Sbjct:   66 IRFGDILVD-------DGLQNADRCTSRKPSCMWPRNSDGNTYIPYIISNQFRPKQKDLITSVLKTFEK--STCIRFKPRDREEDYIDVQSL-EGCYSFVGRTGG-GQTLSLNRRGCLHKK-IIQHELLHALGFHHEQCRSDRDQHIRILYENIIPGEEHNFDKEDTNNLNTPYDYGSVMHYERDAFSRNGQPTMVPIPDQNVAVGRAEEMSANDILRIKKLY 276
[Close]
BLAST of Ast2 protein vs. Ensembl Cavefish
Match: ENSAMXT00000021327.2 (low choriolytic enzyme-like [Source:NCBI gene;Acc:103035835])

HSP 1 Score: 104.76 bits (260), Expect = 3.350e-26
Identity = 63/200 (31.50%), Postives = 98/200 (49.00%), Query Frame = 1 
Query:  271 WPNN-----IVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGH-YIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV-LRKDGGAIGYNHDTTPNDLNMIRNFYGC*RR*KTNI 849
            WP +      V Y  +  F   ++Q +++ L        +C++F+   N   YI + S R GCYS +G  G  +Q ++L+  GC+Y   T++HE +HALGF H   R DRDNHI + WD I+ S    F K        PY+  SVM Y+   F+ + +  +V +     + G     + ND+N +   Y C +    NI
Sbjct:   61 WPKSSDGKVYVPYIISNQFASRELQVIQRGLDSFSS--MSCIRFKRRSNERDYISIES-RSGCYSYIGRLGN-AQTVSLDRNGCIY-HNTVQHELLHALGFNHEQTRSDRDNHIRVVWDNIIDSMKHNFNKIATLNQGTPYDYNSVMQYHRTAFSKNGQPTMVPIPNSNVSFGQASQMSQNDINRLNRLYKCCKYLSVNI 255
[Close]
BLAST of Ast2 protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007253.1 (pep scaffold:Pmarinus_7.0:GL476910:54360:144194:-1 gene:ENSPMAG00000006538.1 transcript:ENSPMAT00000007253.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 78.9518 bits (193), Expect = 1.042e-16
Identity = 59/198 (29.80%), Postives = 87/198 (43.94%), Query Frame = 1 
Query:  259 NSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGH-YIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDG---PYETTSVMHYNSEGFACDPREKVVLRK--DGG---AIGYNHDTTPNDLNMIRNFYGC 825
            + + WP  ++ Y   GNF   +    RQ++   +K    CV F E  +   YI    +  GC S +G  GG  Q +++      +  G + HE  H +GF H H R DRD+H+TI    I   Q   F K +  E+D     Y+  S+MHY    F+       +L +  D G   AIG     +  D+   R  Y C
Sbjct:   18 SERIWPGGVIPYIVGGNFTGSQKAMFRQAMRHWEK--HTCVTFIERTDEESYIVFTYRPCGCCSYVGRRGGGPQAISIGKNCDKF--GIVVHELGHVIGFWHEHTRPDRDDHVTIIKANIQPGQEYNFLKMEPGEVDSLGESYDFDSIMHYARNTFSRGIFLDTILPRPDDSGVRPAIGQRTRLSQGDIAQARKLYRC 211
[Close]
BLAST of Ast2 protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004913.1 (pep scaffold:Pmarinus_7.0:GL478359:22377:26907:1 gene:ENSPMAG00000004462.1 transcript:ENSPMAT00000004913.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 54.6842 bits (130), Expect = 1.571e-9
Identity = 34/100 (34.00%), Postives = 52/100 (52.00%), Query Frame = 1 
Query:  439 CYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDG-PYETTSVMHYNSEGFACDPREK 735
            C+S +G+ G   Q +++   GC    GT+ HE  HA+G++H   R DRD  I++    +   Q  QF      +  G PY+  SVMHY+    AC  R++
Sbjct:    1 CWSFVGMIGVGEQVVSIGR-GCE-GVGTVAHELGHAVGYVHEQSRPDRDAFISVLEGNVQDGQLPQFAVYSATDPRGVPYDLKSVMHYSRNVRACGTRDR 98
[Close]
BLAST of Ast2 protein vs. Ensembl Nematostella
Match: EDO37770 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SEU5])

HSP 1 Score: 102.064 bits (253), Expect = 2.580e-25
Identity = 67/218 (30.73%), Postives = 104/218 (47.71%), Query Frame = 1 
Query:  196 GDMLLDSTDIQIIN-----GFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELD---GPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            GDM+L     Q +N       +    + KKWPN I+ Y    +      + V Q+    + + + CV+FQ+           K +GC+S +G+    +Q ++L   GC + KGT  HE  HALGF H   R DRDN++T+ WD I T     F K     ++    PY+  S+MHY +  F+ + R  +  +K G  IG     +  D++ +   YGC
Sbjct:   34 GDMILTPDQQQAVNNGDLGASRGAPIHDKKWPNAILPYEIRSDLKGTPAEGVIQN-AMREWESKTCVRFQKRTTQKNYVYFFKGDGCWSYVGMLDRGAQAISL-GSGC-WHKGTAAHEIGHALGFYHEQSRPDRDNYVTVHWDNIKTDSKHNFNKYSADRINTFGTPYDFGSLMHYGTNYFSVNGRATITPKKSGAKIGQRDYLSDLDVHQMNLRYGC 248
[Close]
BLAST of Ast2 protein vs. Ensembl Nematostella
Match: EDO42421 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1T1])

HSP 1 Score: 98.2117 bits (243), Expect = 6.057e-25
Identity = 62/194 (31.96%), Postives = 99/194 (51.03%), Query Frame = 1 
Query:  265 KKWPNNIVTYTFAGNFPQDK--IQTVRQSLGELQKDLQNCVKFQE--GRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDG---PYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            K WP  +V Y     F +    ++ + Q++ + +K    C++F++    +  Y+ +   R GC S +G  GG  Q L+L + GC + KGTI HE  HALGF H   R DRD+++TI ++ I     S F K    E+D    PY+  S+MHY  + F+ + +  +V +K G   G     +P D+  +   Y C
Sbjct:   10 KLWPGGVVVYELDPRFERSSRAMEKLTQAISDYEK--YTCIRFRKKTANDNDYVYIKD-RGGCRSHVGRKGG-KQYLSLSS-GC-WHKGTIIHELGHALGFFHEQTRPDRDDYVTILFENIRLGMASNFMKHSAGEVDSLGTPYDYGSIMHYGPKIFSANGKPTIVAKKPGVKFGQRDGLSPIDIKQVNLLYKC 197
[Close]
BLAST of Ast2 protein vs. Ensembl Nematostella
Match: EDO41420 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4I5])

HSP 1 Score: 95.9005 bits (237), Expect = 8.277e-24
Identity = 57/189 (30.16%), Postives = 90/189 (47.62%), Query Frame = 1 
Query:  268 KWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDG---PYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            +WP  ++ YT   +     +  +R  + ++  D   C++F+E  N +      +  GC SM+G TG   Q ++L   GC Y  GT+ HE  HALGF H   R DRDN++TI W+ I  +    F       +D    PY+  SVMHY    F+ + R  +  ++ G  +G     +  D+  +   Y C
Sbjct:   40 RWPEGVMAYTIDRSLSNRAMNAIRAGM-KMWTD-NTCIRFKERTNEYAYARFWRGRGCSSMVGRTG-RRQDISLAG-GCWY-PGTVAHEIGHALGFYHEQSRPDRDNYVTIHWNNISPNMKFNFNMYPRSTIDSLGTPYDYRSVMHYQGNAFSSNGRATITAKQSGVTLGNRRQLSKVDIQQMNLMYKC 223
[Close]
BLAST of Ast2 protein vs. Ensembl Nematostella
Match: EDO34721 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNJ4])

HSP 1 Score: 94.7449 bits (234), Expect = 5.813e-23
Identity = 68/217 (31.34%), Postives = 106/217 (48.85%), Query Frame = 1 
Query:  196 GDMLLDSTDIQIINGFKNHYANS-----KKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRN-GHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELD---GPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFY 819
            GD+ LDS+  +I++   +    +       WPN  + Y F G       +  R ++ E + +   C++F E  +   YI V S+  GC+S +G +GG  Q L+L        KGT  HE +H LGF H   R+DRDN+ITI WD I + +   F K +  ++D    PY+  S+MHY    F+ + R  +V +    +IG     +  DL  +   Y
Sbjct:    4 GDIYLDSSTTRIMDQLMSPSREALVDPVSHWPNGRLPYKF-GAVSNSVKEAFRAAIDEYEAN--TCIRFVERTDETDYILVVSE-GGCWSPIGRSGG-QQKLSLGT--YCDRKGTAMHELMHTLGFFHEQSRRDRDNYITIHWDNIQSDKQFNFQKYEHGKVDTLGAPYDYGSIMHYPMFAFSSNGRATIVPKDPTASIGQRSGFSKVDLQQLNKLY 213
[Close]
BLAST of Ast2 protein vs. Ensembl Nematostella
Match: EDO30445 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T0S0])

HSP 1 Score: 85.8853 bits (211), Expect = 1.009e-19
Identity = 67/217 (30.88%), Postives = 106/217 (48.85%), Query Frame = 1 
Query:  184 NVKMGDMLLDSTDIQIINGFKNHYAN-SKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGH-YIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDG---PYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFY 819
            +V  GD+ LD +   I+   ++  ++ ++ W N  V Y F G+   +       ++ E +   + CV+F   R    YI + S+  GC+S +G +GG  Q L+L   GC + +GT  HE +HA+GF H   R DRD++ITI WD I       F K    E D     Y+  S+MHY    F  + R+  ++ ++G  IG     +  D+  I   Y
Sbjct:    1 DVFEGDIYLDRSTKAILGMGRDAMSSPARHWLNARVPYVF-GSVSSEVRSVFLAAIKEYEA--KTCVRFVPRRGERDYIYIVSE-GGCFSSIGRSGG-LQKLSL-GTGCEH-RGTAIHEMMHAIGFFHEQSRLDRDSYITIYWDNIKPGMEYNFRKYKRGEADTLGYAYDLKSIMHYPKYAFTKN-RQPTIIARNGANIGQRDSFSAIDIQQINALY 209
[Close]
BLAST of Ast2 protein vs. Ensembl Medaka
Match: mc6ast5 (astacin like metallo-protease [Source:NCBI gene;Acc:100049509])

HSP 1 Score: 109.768 bits (273), Expect = 5.479e-28
Identity = 66/194 (34.02%), Postives = 96/194 (49.48%), Query Frame = 1 
Query:  259 NSKKWPNNIVTYTFAGNFPQ---DKIQTVRQSLGELQKDLQNCVKF-QEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGG-AIGYNHDTTPNDLNMIRNFYGC 825
            N+  WP ++  + +         D +  +    G        C+KF       H++++   R GC+S LG TGG SQ L+L+ PGCM+S G   HEF+HALGF+H   R DRD+++TI W  I+      F K     L+ PY+ +SVMHY    F+ D    ++ R D    IG     +  DL+ I   Y C
Sbjct:   89 NTCLWPKSVDGFVYVPYIVSPLYDDMDRITIETGMQDIASGTCIKFVPRTHEAHFLDIQ-PRYGCWSFLGQTGG-SQTLSLQTPGCMWS-GIAAHEFMHALGFVHEQSRSDRDHYVTIVWKNIMPDHAHNFRKQATNNLNSPYDYSSVMHYGRYAFSGDGGPTIIPRPDPYIPIGQRDGPSALDLHKINVLYDC 279
[Close]
BLAST of Ast2 protein vs. Ensembl Medaka
Match: mc6ast4 (astacin like metallo-protease [Source:NCBI gene;Acc:100049317])

HSP 1 Score: 104.375 bits (259), Expect = 5.866e-26
Identity = 65/219 (29.68%), Postives = 109/219 (49.77%), Query Frame = 1 
Query:  196 GDMLLDSTDIQIINGFKNH--YANSKKWPNNI-----VTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKF-QEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGA-IGYNHDTTPNDLNMIRNFYGC 825
            G   L   DI +  G ++   +A S  W  ++     + YT + ++ +  ++ +++ +  +++    CV+F  +     Y+++  K  GC+S LG  GG  Q L+L+NP CM   G I HEF+HALGF+H   R DRDN+IT+ W  I   +   F K     LD PY+  S+MH+    ++ D +  +V + +    +G     +  D   I   Y C
Sbjct:   62 GTTTLREGDIAVSTGRRSKMCFARSCLWSKSVDGHVYIAYTISPDYTEADVKLIKKGMENIEEG--TCVRFVPQTHQRDYVDIQPK-SGCWSYLGARGG-RQTLSLQNPDCM-QVGVISHEFMHALGFVHEQSRFDRDNYITVMWPNIWRDRLRNFEKFKTDSLDLPYDYGSIMHFGMYAYSMDGQPTLVAKNNRNMKLGQASSLSHTDKLKINRLYQC 275
[Close]
BLAST of Ast2 protein vs. Ensembl Medaka
Match: lce (protease [Source:NCBI gene;Acc:100049451])

HSP 1 Score: 103.99 bits (258), Expect = 7.006e-26
Identity = 70/229 (30.57%), Postives = 106/229 (46.29%), Query Frame = 1 
Query:  160 KLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYA-NSKKWP---NNIVT--YTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV-LRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            ++    ++ +  GD++L  T     N  K   A +S +WP   N IV   Y  + N+  D+ +T+R ++ E  +  + C+ F    N         R GC SM+G  G   Q + L+  GC+     I+HE +HALGF H H R DRD H+ I W+ I+      F K D   L  PY+  S+MHY    F  D +E +  +     AIG     +  D+  +   Y C
Sbjct:   51 RMNNNSMEELLEGDLVLPKTR----NAMKCFGAPDSCRWPKSSNGIVKVPYVVSDNYESDEKETIRNAMKEFAE--KTCIHFVPRNNERAYLSLEPRFGCKSMMGYVGD-KQVVVLQRFGCI-KHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLGTPYDYGSIMHYGRTAFGKDRKETITPIPNPKAAIGQTERMSDIDILRVNKLYKC 271
[Close]
BLAST of Ast2 protein vs. Ensembl Medaka
Match: lce (protease [Source:NCBI gene;Acc:100049451])

HSP 1 Score: 103.605 bits (257), Expect = 8.439e-26
Identity = 70/229 (30.57%), Postives = 106/229 (46.29%), Query Frame = 1 
Query:  160 KLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYA-NSKKWP---NNIVT--YTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVV-LRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            ++    ++ +  GD++L  T     N  K   A +S +WP   N IV   Y  + N+  D+ +T+R ++ E  +  + C+ F    N         R GC SM+G  G   Q + L+  GC+     I+HE +HALGF H H R DRD H+ I W+ I+      F K D   L  PY+  S+MHY    F  D +E +  +     AIG     +  D+  +   Y C
Sbjct:   51 RMNNNSMEELLEGDLVLPKTR----NAMKCFGAPDSCRWPKSSNGIVKVPYVVSDNYESDEKETIRNAMKEFAE--KTCIHFVPRNNERAYLSLEPRFGCKSMMGYVGD-KQVVVLQRFGCI-KHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLGTPYDYGSIMHYGRTAFGKDRKETITPIPNPKAAIGQTERMSDIDILRVNKLYKC 271
[Close]
BLAST of Ast2 protein vs. Ensembl Medaka
Match: ENSORLT00000018637.2 (pep primary_assembly:ASM223467v1:3:32702055:32705063:-1 gene:ENSORLG00000014863.2 transcript:ENSORLT00000018637.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 99.3673 bits (246), Expect = 3.357e-24
Identity = 53/146 (36.30%), Postives = 81/146 (55.48%), Query Frame = 1 
Query:  283 IVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGH-YIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFA 717
            ++ Y  +  + + ++ T+  ++       + C++F    N + +I V SK  GCYS LG  GG  Q L+L   GCMYS G I+HE  HALGF H   R DRD+++ I W  I+ +    F K D   L+ PY+ +S+MHY  + F+
Sbjct:   91 VIPYVISSQYSRGEVATIEGAMRAFNG--RTCIRFVRRTNEYDFISVVSK-NGCYSELGRKGG-QQELSLNRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWQNIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFS 231
[Close]
BLAST of Ast2 protein vs. Planmine SMEST
Match: SMESG000072807.1 (SMESG000072807.1)

HSP 1 Score: 457.603 bits (1176), Expect = 8.714e-165
Identity = 216/238 (90.76%), Postives = 223/238 (93.70%), Query Frame = 1 
Query:  112 VIDYANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            +I Y      C  QSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCE DGPYET SVMHYNSEGFAC+P EKVV++K+GGAIGYNH+TT NDL MI+NFY C
Sbjct:    7 IIYYYQASSFCLIQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQMIKNFYNC 244
[Close]
BLAST of Ast2 protein vs. Planmine SMEST
Match: SMESG000072805.1 (SMESG000072805.1)

HSP 1 Score: 457.603 bits (1176), Expect = 8.714e-165
Identity = 216/238 (90.76%), Postives = 223/238 (93.70%), Query Frame = 1 
Query:  112 VIDYANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            +I Y      C  QSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCE DGPYET SVMHYNSEGFAC+P EKVV++K+GGAIGYNH+TT NDL MI+NFY C
Sbjct:    7 IIYYYQASSFCLIQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQMIKNFYNC 244
[Close]
BLAST of Ast2 protein vs. Planmine SMEST
Match: SMESG000072807.1 (SMESG000072807.1)

HSP 1 Score: 457.218 bits (1175), Expect = 1.302e-164
Identity = 216/238 (90.76%), Postives = 223/238 (93.70%), Query Frame = 1 
Query:  112 VIDYANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            +I Y      C  QSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCE DGPYET SVMHYNSEGFAC+P EKVV++K+GGAIGYNH+TT NDL MI+NFY C
Sbjct:    9 IIYYYQASSFCLIQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQMIKNFYNC 246
[Close]
BLAST of Ast2 protein vs. Planmine SMEST
Match: SMESG000072805.1 (SMESG000072805.1)

HSP 1 Score: 457.218 bits (1175), Expect = 1.302e-164
Identity = 216/238 (90.76%), Postives = 223/238 (93.70%), Query Frame = 1 
Query:  112 VIDYANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            +I Y      C  QSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCE DGPYET SVMHYNSEGFAC+P EKVV++K+GGAIGYNH+TT NDL MI+NFY C
Sbjct:    9 IIYYYQASSFCLIQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQMIKNFYNC 246
[Close]
BLAST of Ast2 protein vs. Planmine SMEST
Match: SMESG000072807.1 (SMESG000072807.1)

HSP 1 Score: 448.358 bits (1152), Expect = 2.732e-161
Identity = 214/238 (89.92%), Postives = 221/238 (92.86%), Query Frame = 1 
Query:  112 VIDYANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 825
            +I Y      C  QSVKLRTRGLDNVKMGDMLLDSTDIQIING  NHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCE DGPYET SVMHYNSEGFAC+P EKVV++K+GGAIGYNH+TT NDL MI+NFY C
Sbjct:    7 IIYYYQASSFCLIQSVKLRTRGLDNVKMGDMLLDSTDIQIING--NHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQMIKNFYNC 242
The following BLAST results are available for this feature:
BLAST of Ast2 protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ASTL2.366e-1928.32astacin like metalloendopeptidase [Source:HGNC Sym... [more]
MEP1B3.983e-1731.25meprin A subunit beta [Source:HGNC Symbol;Acc:HGNC... [more]
MEP1B4.023e-1731.25meprin A subunit beta [Source:HGNC Symbol;Acc:HGNC... [more]
TLL17.520e-1629.59tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843][more]
TLL12.316e-1529.90tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843][more]
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BLAST of Ast2 protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nas-42.074e-2630.87Zinc metalloproteinase nas-4 [Source:UniProtKB/Sw... [more]
nas-45.294e-2630.87Zinc metalloproteinase nas-4 [Source:UniProtKB/Sw... [more]
nas-45.368e-2630.87Zinc metalloproteinase nas-4 [Source:UniProtKB/Sw... [more]
nas-65.936e-2433.17Zinc metalloproteinase nas-6 [Source:UniProtKB/Sw... [more]
nas-154.986e-2232.06Zinc metalloproteinase nas-15 [Source:UniProtKB/S... [more]
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BLAST of Ast2 protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG67636.003e-3532.24gene:FBgn0039069 transcript:FBtr0084378[more]
CG67636.003e-3532.24gene:FBgn0039069 transcript:FBtr0334497[more]
CG118651.842e-2327.95gene:FBgn0028947 transcript:FBtr0080753[more]
CG152551.708e-2229.13gene:FBgn0028950 transcript:FBtr0080785[more]
CG152551.708e-2229.13gene:FBgn0028950 transcript:FBtr0343719[more]
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BLAST of Ast2 protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hce2l19.183e-2631.79high choriolytic enzyme 2-like 1 [Source:ZFIN;Acc:... [more]
hce2l12.877e-2532.49high choriolytic enzyme 2-like 1 [Source:ZFIN;Acc:... [more]
hce2l23.239e-2429.85high choriolytic enzyme 2-like 2 [Source:ZFIN;Acc:... [more]
hce2l23.303e-2429.23high choriolytic enzyme 2-like 2 [Source:ZFIN;Acc:... [more]
c6ast33.309e-2431.32six-cysteine containing astacin protease 3 [Source... [more]
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BLAST of Ast2 protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
polr2l.22.949e-2432.86polymerase (RNA) II (DNA directed) polypeptide L, ... [more]
kpna41.138e-2230.30karyopherin alpha 4 (importin alpha 3) [Source:Xen... [more]
ENSXETT00000032332.11.450e-2131.94astacin-like metallo-endopeptidase 3 gene b1 [Sour... [more]
ENSXETT00000032191.11.748e-2131.94astacin-like metallo-endopeptidase 3 gene b1 [Sour... [more]
ENSXETT00000032173.11.868e-2131.94astacin-like metallo-endopeptidase 3 gene b1 [Sour... [more]
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BLAST of Ast2 protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Mep1b5.270e-1729.60meprin 1 beta [Source:MGI Symbol;Acc:MGI:96964][more]
Astl4.230e-1625.00astacin-like metalloendopeptidase (M12 family) [So... [more]
Astl5.168e-1625.00astacin-like metalloendopeptidase (M12 family) [So... [more]
Astl5.168e-1625.00astacin-like metalloendopeptidase (M12 family) [So... [more]
Tll28.719e-1627.16tolloid-like 2 [Source:MGI Symbol;Acc:MGI:1346044][more]
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BLAST of Ast2 protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P55112|NAS4_CAEEL2.638e-2530.87Zinc metalloproteinase nas-4 OS=Caenorhabditis ele... [more]
sp|P31579|LCE_ORYLA7.564e-2530.57Low choriolytic enzyme OS=Oryzias latipes OX=8090 ... [more]
sp|P31581|HCE2_ORYLA3.075e-2336.30High choriolytic enzyme 2 OS=Oryzias latipes OX=80... [more]
sp|Q9U3S9|NAS6_CAEEL7.550e-2333.17Zinc metalloproteinase nas-6 OS=Caenorhabditis ele... [more]
sp|P31580|HCE1_ORYLA1.168e-2235.62High choriolytic enzyme 1 OS=Oryzias latipes OX=80... [more]
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BLAST of Ast2 protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A060Q7149.353e-18095.20Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7851.214e-15180.88Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7863.947e-12768.80Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
I1ZIC34.797e-9253.20Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7503.828e-7145.85Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
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BLAST of Ast2 protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hce2l11.036e-2833.68high choriolytic enzyme 1-like [Source:NCBI gene;A... [more]
c6ast15.548e-2834.21low choriolytic enzyme-like [Source:NCBI gene;Acc:... [more]
c6ast17.027e-2834.21low choriolytic enzyme-like [Source:NCBI gene;Acc:... [more]
npsn1.063e-2633.18low choriolytic enzyme-like [Source:NCBI gene;Acc:... [more]
ENSAMXT00000021327.23.350e-2631.50low choriolytic enzyme-like [Source:NCBI gene;Acc:... [more]
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BLAST of Ast2 protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000007253.11.042e-1629.80pep scaffold:Pmarinus_7.0:GL476910:54360:144194:-1... [more]
ENSPMAT00000004913.11.571e-934.00pep scaffold:Pmarinus_7.0:GL478359:22377:26907:1 g... [more]
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BLAST of Ast2 protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ast2 protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO377702.580e-2530.73Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO424216.057e-2531.96Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO414208.277e-2430.16Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO347215.813e-2331.34Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO304451.009e-1930.88Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Ast2 protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mc6ast55.479e-2834.02astacin like metallo-protease [Source:NCBI gene;Ac... [more]
mc6ast45.866e-2629.68astacin like metallo-protease [Source:NCBI gene;Ac... [more]
lce7.006e-2630.57protease [Source:NCBI gene;Acc:100049451][more]
lce8.439e-2630.57protease [Source:NCBI gene;Acc:100049451][more]
ENSORLT00000018637.23.357e-2436.30pep primary_assembly:ASM223467v1:3:32702055:327050... [more]
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BLAST of Ast2 protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000072807.18.714e-16590.76SMESG000072807.1[more]
SMESG000072805.18.714e-16590.76SMESG000072805.1[more]
SMESG000072807.11.302e-16490.76SMESG000072807.1[more]
SMESG000072805.11.302e-16490.76SMESG000072805.1[more]
SMESG000072807.12.732e-16189.92SMESG000072807.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30012714
GenBankCCW36840.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012714 ID=SMED30012714|Name=Ast2 protein|organism=Schmidtea mediterranea sexual|type=transcript|length=864bp
TGGAGACGAACTTCCTGTTTACGTTGCTGCAAGAGAATCGTATAAAAACC
GGAAGCATTCAGTATCAACACATTCATGGAAAAATTAATTCTCTTACTTT
TAGGATTTGTTGTGATTGACTATGCCAACGGCCAATGTGGATGTCCACGT
CAATCGGTGAAGCTGAGAACGAGAGGTCTCGACAATGTCAAGATGGGAGA
CATGCTTTTGGATTCCACAGATATACAGATCATCAATGGGTTCAAGAATC
ATTACGCAAACAGCAAGAAGTGGCCCAACAACATCGTCACGTATACATTT
GCAGGAAACTTCCCTCAAGACAAGATCCAAACTGTGCGACAATCTCTAGG
AGAATTACAGAAAGATTTACAAAACTGTGTCAAGTTTCAAGAAGGAAGAA
ACGGACATTACATCGAAGTCAACTCGAAACGTGAAGGATGTTATTCTATG
TTGGGAGTCACAGGAGGTCCTAGTCAGCCGTTGAATCTTGAAAACCCTGG
ATGTATGTATTCAAAAGGAACGATCAAACACGAGTTTATCCACGCCCTCG
GATTCATGCACACCCACATGAGAAAGGACAGAGACAACCACATCACTATC
AAATGGGACAGAATCCTCACAAGTCAATGCAGTCAGTTTACGAAATGCGA
CGGATGTGAACTTGATGGACCATACGAAACCACCAGTGTAATGCACTATA
ACAGTGAAGGATTTGCTTGTGATCCTCGAGAAAAAGTTGTTTTAAGAAAA
GATGGAGGAGCGATCGGTTATAATCACGACACAACTCCTAATGATTTGAA
CATGATAAGAAACTTTTATGGATGTTAAAGACGTTGAAAAACAAATATAT
GTAAAATTTGCTTG
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protein sequence of SMED30012714-orf-1

>SMED30012714-orf-1 ID=SMED30012714-orf-1|Name=SMED30012714-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=251bp
MEKLILLLLGFVVIDYANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDI
QIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQN
CVKFQEGRNGHYIEVNSKREGCYSMLGVTGGPSQPLNLENPGCMYSKGTI
KHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCELDGPY
ETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC
*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000420parapharyngeal region
PLANA:0000429neoblast
Vocabulary: INTERPRO
TermDefinition
IPR001506Peptidase_M12A
IPR006026Peptidase_Metallo
IPR024079MetalloPept_cat_dom_sf
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
Vocabulary: molecular function
TermDefinition
GO:0004222metalloendopeptidase activity
GO:0008270zinc ion binding
GO:0008237metallopeptidase activity
GO:0008233peptidase activity
GO:0016787hydrolase activity
GO:0046872metal ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001506Peptidase M12APRINTSPR00480ASTACINcoord: 163..180
score: 41.76
coord: 144..162
score: 45.61
IPR001506Peptidase M12APFAMPF01400Astacincoord: 64..181
e-value: 1.3E-28
score: 99.9
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 62..183
e-value: 1.8E-17
score: 74.1
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3DG3DSA:3.40.390.10coord: 20..183
e-value: 4.5E-34
score: 119.9
NoneNo IPR availablePANTHERPTHR10127DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAININGcoord: 38..181
NoneNo IPR availablePROSITEPS51864ASTACINcoord: 54..183
score: 36.273
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..19
score: 0.808
NoneNo IPR availableSUPERFAMILYSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 62..181