SMED30012698

Overview


Name	SMED30012698
Smed ID	SMED30012698
Length (bp)	4278

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30012698 (SMED30012698) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30012698 (SMED30012698) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30012698 (SMED30012698) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30012698 (SMED30012698) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30012698

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 1

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication	Specimen	Lifecycle	Evidence
parenchyma	SMED30012698		dd_Smed_v4_17687_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of SMED30012698 vs. TrEMBL
Match: A0A4C1X7C4 (BED-type domain-containing protein OS=Eumeta variegata OX=151549 GN=EVAR_25855_1 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 9.451e-20
Identity = 83/250 (33.20%), Postives = 126/250 (50.40%), Query Frame = -3

Query: 1454 IGLDRIFR*CTATKLLKVVTYHLGSFKVNYEQHVVGTTGDSASLMVIHGKECPTSYKLCLNHGLHLVVCDTLXXXXXXXXXXX------XXXXXXXXXXXTKDSIQK-----------------IDCIHEIIKNVRKVVKFFRLSPF---ILQEKIRAKLGHNLELYLDCKTRWSSLANMXXXXXXXXXXXXEALVDLNFSHIIDHI----NFNELKDLQNSLEHAKLAIEVLIGNDTNLHSVRITLEYM 2113
            +GL RIF    AT  ++ +   L  F+++ ++ +V  T D AS+MV  G   P   +LC  HGL L + D LYKK      E       +  + E+     + +I +                 I    +II  VRK+V+ F+ SP    +LQ+ ++ + G  +EL  DCKTRWSSLA M+  F KL+  I + L+DL  S  I++      F+ L +L+N L+  KLA+EVL   D  L +   TL++M
Sbjct:  643 LGLTRIFGSMPATVCVETLRKKLKKFEIDLDEDIVAITTDGASVMVKTGSLVPAFQQLCYAHGLQLGILDVLYKKNESIRQELIDDDILDNSEAESNDNDGRPNISENSGFTVEVVSFATEGELIPDFKDIINKVRKIVRMFKCSPTKNDVLQKYVKNEFGRKIELDGDCKTRWSSLAVMVEKFNKLKLCIAKTLIDLGLSANIEYRFSQHEFDALVNLENILKPVKLAVEVLCRQDATLITTEATLKFM 892

HSP 2 Score: 31.5722 bits (70), Expect = 9.451e-20
Identity = 12/24 (50.00%), Postives = 16/24 (66.67%), Query Frame = -1

Query: 2137 RFSFTLDEWASLRTRKYINVILHS 2208
            RFS T DEW S   R+Y+N+  H+
Sbjct:  609 RFSLTFDEWTSSSNRRYLNINAHT 632

[Close]

BLAST of SMED30012698 vs. TrEMBL
Match: B0W9I7 (Predicted protein OS=Culex quinquefasciatus OX=7176 GN=6035136 PE=4 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.415e-17
Identity = 99/346 (28.61%), Postives = 168/346 (48.55%), Query Frame = -3

Query: 1517 ELEDVDS---EVGCSIKKPKTIMDFVKRSSLEKIISKLEPVDGISIRVISRL*FIRESIYNSGYKLLKNENDVMQLIHDHFEGMKEXXXXXXXKNYK*PEI*LHT**VGQLT---NEEIYQCNSP*CPRSI*KIIGLDRIFR*CTATKLLKVVTYHLGSFKVNYEQHVVGTTGDSASLMVIHGKECPTSYKLCLNHGLHLVVCDTLXXXXXXXXXXXXXXXXXXXXXXTKDSIQKIDCI----------HEIIKNVRKVVKFFRLSPF---ILQEKIRAKLGHNLELYLDCKTRWSSLANMXXXXXXXXXXXXEALVDLNFSHIIDHINFNELKDLQNSLEHAKLA 2497
            + EDV S   +   +  K  TI  F+ +  + ++ ++L   DG++I  I+   FIR S    GY+L  ++  VM+LIHD++E  K++ ++ I+   K  E+   +  + + T   N +    N      +   + GL R+     A K+L+ V  HL  F +  +++V   T D+A +M   GK     ++LC NHGLHL VCD ++K      +  E  D + E   +++S    D              ++  +R + K FR SP    ILQ+K+  + G  L+L LD KTRW+S   M+  + KL   + EAL +L     +   +   +  L ++L+  +LA
Sbjct:   57 QPEDVPSGSKQAKSNTSKQSTIEPFIHKKPMNEMFARLAAQDGLTISAITNSKFIRTSFAQRGYQLPLSKTSVMKLIHDYYEIAKQQTVQQIQD--KLAELHFLSITLDEWTSSGNRKYLNVNVHFGDGAFFNL-GLVRVKGSFPAEKILEAVDQHLECFAIR-DRNVAAATSDAAKVMEKFGKLATFKHQLCFNHGLHLAVCDVIFKSSETPGDLLEESDSDDEDCDSEESDDSKDATIPGLYNSSNYRPVLLQIRNICKLFRRSPVKNAILQKKVVQEFGKPLQLILDVKTRWNSTEQMLARYMKLHGCVAEALKELEDLAPVSTSDLESISQLYDALQPDELA 398

[Close]

BLAST of SMED30012698 vs. TrEMBL
Match: A0A4Y2LA02 (Uncharacterized protein OS=Araneus ventricosus OX=182803 GN=AVEN_237838_1 PE=4 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 1.355e-16
Identity = 79/248 (31.85%), Postives = 120/248 (48.39%), Query Frame = -3

Query: 1454 IGLDRIFR*CTATKLLKVVTYHLGSFKVNYEQHVVGTTGDSASLMVIHGKECPTSYKLCLNHGLHLVVCDTLXXXXXXXXXXXXXXXXXXXXXXTKDS------------IQKIDCIH------EIIK---NVRKVVK---FFRLSP----FILQEKIRAKLGHNLELYLDCKTRWSSLANMXXXXXXXXXXXXEALVDLNFSHIIDHINFNELKDLQNSLEHAKLAIEVLIGNDTNLHSVRITLEYM 2113
            +GL RI   CT    + ++   L SF ++ +  ++G T D AS+MV  GK     ++LC +HGLHL V D LYKK    E   + I          D                 D  H      E+I    +++KV K    F+ SP      LQ+ ++      L L LDC+TRW+ L  MI  F KL+  I++AL+D+        + + ++KDL +SL+ +KLAIE L   D+ L +   TL+++
Sbjct:  190 LGLIRIHGSCTLEHTISLIKNRLESFGLDSDTDIIGVTTDGASVMVKVGKLMSCYHQLCFSHGLHLAVVDILYKKNIEREEAHQEITSNESDTDDDDDTNDTHEEQGVTVTTTTDPRHLHLSRAEVIPRYNDLQKVRKVVKLFKRSPTKYDTYLQKYVKEDTCKQLSLILDCRTRWNGLLAMIERFHKLKVCIDKALIDMGSDTKFSDLEWTKIKDLIDSLQPSKLAIEALCRRDSTLLTAETTLKFI 437

HSP 2 Score: 31.5722 bits (70), Expect = 1.355e-16
Identity = 21/86 (24.42%), Postives = 43/86 (50.00%), Query Frame = -3

Query: 2276 DIHPNLDKHELEDVD--SEVGCSIKKPKTIMDFVKR-SSLEKIISKLEPVDGISIRVISRL*FIRESIYNSGYKLLKNENDVMQLI 2524
            D+ P     + + VD   E     KK K I  + K+ +S+EK++S++   DG+++ +      +R     SG++L  + N +  ++
Sbjct:   51 DLFPKASTSKTDAVDIICEEEIPKKKSKKINSYFKKETSMEKMVSRMACKDGVALSLFCSSSDLRYLFSKSGFQLPNSSNTIRSIV 136

HSP 3 Score: 27.7202 bits (60), Expect = 1.355e-16
Identity = 9/23 (39.13%), Postives = 16/23 (69.57%), Query Frame = -1

Query: 2140 RFSFTLDEWASLRTRKYINVILH 2208
            RF+ + DEW S +  +Y+N+ +H
Sbjct:  160 RFALSFDEWTSQKNHRYLNLRVH 182

[Close]

BLAST of SMED30012698 vs. TrEMBL
Match: A0A4C1WY75 (Uncharacterized protein OS=Eumeta variegata OX=151549 GN=EVAR_35857_1 PE=4 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 4.597e-16
Identity = 84/256 (32.81%), Postives = 132/256 (51.56%), Query Frame = -3

Query: 1433 IGLDRIFR*CTATKLLKVVTYHLGSFKVNYEQHVVGTTGDSASLMVIHGKECPTSYKLCLNHGLHLVVCDTLXXXXXXXXXXXXXXXXXXXXXXTKD-------------------SIQKIDCIHE---IIKNVRKVVKFFRLSPF---ILQEKIRAKLGHNLELYLDCKTRWSSLANMXXXXXXXXXXXXEALVDLNFSHIIDHINFNE----LKDLQNSLEHAKLAIEVLIGNDTNLHSVRITLEYMKNFMKNI 2113
            IGL RIF   ++   ++++   L  F ++ +  +VG T D AS+M   G+      +LC  HG+ L + D +YKK   S  EEE  D E     + +                    I+++D   E   II  +RK VK F+ SP    +LQ  I+ + G  ++L LDCKTRWSS+ NMI  + ++++ + + LVDL+ S   ++   +E    L DL+N+L+  KLA+EVL   D +L S   TL +M   + N+
Sbjct:   50 IGLARIFGSMSSESCVQLLKEKLSEFDLSLDNDIVGITTDGASVMKKVGRLIEPLQQLCYAHGVQLGITDVIYKKTVQSNPEEEFEDPELNTNRSAEEEEVLGDNPDNYDGLYFSLPIRQVDLGTEYQMIISKLRKCVKLFKNSPTKNDMLQIYIQQEFGKTIQLVLDCKTRWSSICNMISTYNRVKQCVAKTLVDLSLSSNPEYFFTDEEHAVLIDLENTLQPVKLAVEVLCRQDIDLVSAEHTLRFMVRKLGNL 305

[Close]

BLAST of SMED30012698 vs. TrEMBL
Match: A0A0N7Z9Z3 (BED-type domain-containing protein OS=Scylla olivacea OX=85551 PE=4 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 5.338e-16
Identity = 107/384 (27.86%), Postives = 161/384 (41.93%), Query Frame = -3

Query: 1592 TTSLFKHLK-------THDIHPNLDKHELEDVDSEVGCSIKKPKTIMDFVKRSSLEKIISKLEPVDGISIRVISRL*FIRESIYNSGYKLLKNENDVMQLIHDHFEGMKEXX-------------------XXXXXKNYK*PEI*LHT**VGQLTNEEIYQCNSP*CPRSI*KIIGLDRIFR*CTATKLLKVVTYHLGSFKVNYEQHVVGTTGDSASLMVIHGKECPTSYKLCLNHGLHLVVCDTLXXXXXXXXXXXXXXXXXXXXXXTKDSIQKIDCIHEI---------------------------------IKNVRKVVKFFRLSPF---ILQEKIRAKLGHNLELYLDCKTRWSSLANMXXXXXXXXXXXXEALVDLNF 2557
            T+ + +HLK       T ++    D+   +D  SEV  +  + K      K  + E+I+ KL  VDG SI  I++  FI+ S+   GYKL ++   VM L+H  +   KE++                        KN +   I LHT       ++  +              +G+ RI     A   ++VV   L  F+VN E+ VV    D AS+MV  GK   T +  C  HG+HL VCD LYKK   +    E  +     E   D ++ +    E+                                 I  VRK+VK FR SP     LQ  + ++ G  L L LD KTRW+SL +M+  F K++  + +A++D N 
Sbjct:   44 TSGMIRHLKSKHSIEKTENLKRPSDRSHDKDT-SEVKRNSVQQKMTDLLKKEETKEEIVGKLAAVDGFSINAIAKSEFIKTSMLARGYKLPQSPTLVMDLVHKQYNVAKERVIFDINKRKNVGVRFGLSLDEYTSLKNKRYMNINLHT-------SDTFWN-------------LGMARITGSVPAETAVEVVENKLAEFQVNLERDVVACVTDGASVMVKFGKLIKTCHHTCYAHGIHLAVCDVLYKKTDGNLVSLERRNVTHLQEEEADDVEAVPEEEELSSMIEVVSDEDLENIRLEDLEDDEVGNIDLSVTINKVRKIVKIFRKSPTRNEKLQMYVVSEYGKELMLKLDSKTRWNSLLDMLERFLKVKNSVAKAMIDCNM 406

[Close]

BLAST of SMED30012698 vs. Planmine SMEST
Match: SMESG000004539.1 (SMESG000004539.1)

HSP 1 Score: 105.916 bits (263), Expect = 4.799e-23
Identity = 73/199 (36.68%), Postives = 111/199 (55.78%), Query Frame = -3

Query: 1904 DVDSEVGCSIKKP-KTIMDFVKRSSLEKIISKLEPVDGISIRVISRL*FIRESIYNSGYKLLKNENDVMQLIHDHF-EGMKEXXXXXXXKNYK*PEI*LHT**VGQLTNEEIYQCNSP*CPRSI*KI--IGLDRIFR*CTATKLLKVVTYHLGSFKVNYEQHVVGTTGDSASLMVIHGKECPTSYKLCLNHGLHLVVCD 2488
            D DS+   S +   KTI+DF+K+ +L +I+ +L   DG SIR I+R  FIR++I+  GYKL KNEN+ + +  ++F E +K+     I               + +L +   ++  +        K+  +GL RI   CT  K+  +VT HL  F +++E  VV TT D  S+MV +G+E P   +LCLNHGLHL VC+
Sbjct:   14 DSDSKASTSTRSHHKTIVDFMKKITLGEIVDELATKDGFSIRGITRSSFIRQAIHAQGYKLPKNENENIYI--NYFDEAIKKGQKFSIT--------------LDELVSSRQHRYMNINVHDKTGKVFNLGLIRIIGSCTTRKIPSIVTDHLKVFHLSFENDVVATTNDGTSVMVKYGRESPAEVQLCLNHGLHLDVCN 196

[Close]

BLAST of SMED30012698 vs. Planmine SMEST
Match: SMESG000046976.1 (SMESG000046976.1)

HSP 1 Score: 100.523 bits (249), Expect = 2.131e-22
Identity = 82/230 (35.65%), Postives = 118/230 (51.30%), Query Frame = -3

Query: 1454 IGLDRIFR*CTATKLLKVVTYHLGSFKVNYEQHVVGTTGDSASLMVIHGKECPTSYKLCLNHGLHLVVCDTLXXXXXXXXXXXXXXXXXXXXXXTKDSIQKIDCIHEIIKN-------VRKVVKFFRLSPF---ILQEKIRAKLGHNLELYLDCKTRWSSLANMXXXXXXXXXXXXEALVDLNFSHIIDHINFNELKDLQNSLEHAKLAIEVLIGNDTNLHSVRITLEYM 2113
            +GL RI   C A KLL +VT HL  F +++E  VV    D AS+MV +G+E     +LCLNHGLHL VC+T Y KK                    +  Q      E I N         + +KFF+ S     ILQ K+R  L H L L LD K RW+S+  M+  F KL+K I EAL +LN ++++  +N + +K L + L+  +L +  L   D NL + +  + ++
Sbjct:   13 LGLIRIIGSCNARKLLSIVTDHLKIFHLSFENDVVANINDGASVMVKYGRESKAEVQLCLNHGLHLAVCNTFYFKKCI------------------NDYQNTSSEDEAIGNNLSATTDEYEEIKFFKSSTIRNDILQAKVRIGLEHELHLVLDVKHRWNSIYPMLEQFLKLQKPIVEALTELNATNLLIGLNLDVIKGLCSILQPLELTVLALSWKDANLITAKGAINFL 224

[Close]

BLAST of SMED30012698 vs. Planmine SMEST
Match: SMESG000068156.1 (SMESG000068156.1)

HSP 1 Score: 79.7221 bits (195), Expect = 6.828e-17
Identity = 52/143 (36.36%), Postives = 80/143 (55.94%), Query Frame = -3

Query: 1700 IGLDRIFR*CTATKLLKVVTYHLGSFKVNYEQHVVGTTGDSASLMVIHGKECPTSYKLCLNHGLHLVVCDTLXXXXXXXXXXXXXXXXXXXXXXTKDS--IQKIDCIHEIIKNVRKVVKFFR---LSPFILQEKIRAKLGHNL 2113
            +GL RI   C A K+L +VT HL  F +++E   + T  D AS+MV +G+E P   +LCLNHGLHL V + +   +     +E  +   T+    +++  + +I  I EI+  VR +VK F+   +   ILQ K+R +L H L
Sbjct:   59 LGLIRIIGSCNARKILSIVTDHLKIFHLSFENADMATINDGASVMVKYGRESPAEVQLCLNHGLHLAVYEAIGNYEEDEFEDEFSLSATTDEYEEREAKRVTEIFKIAEILNKVRVIVKIFKSLTVRNDILQAKVRIELEHEL 201

HSP 2 Score: 28.4906 bits (62), Expect = 6.828e-17
Identity = 12/28 (42.86%), Postives = 17/28 (60.71%), Query Frame = -1

Query: 2140 ITNNLRFSFTLDEWASLRTRKYINVILH 2223
            I    +FS TLDE  S R  +Y+N+ +H
Sbjct:   23 IKKGQKFSITLDECLSSRQNRYMNINVH 50

[Close]

BLAST of SMED30012698 vs. Planmine SMEST
Match: SMESG000077975.1 (SMESG000077975.1)

HSP 1 Score: 79.337 bits (194), Expect = 7.087e-15
Identity = 100/410 (24.39%), Postives = 184/410 (44.88%), Query Frame = -3

Query: 1439 KAVDSITTSLFKHLKT-HDIHPNLDKHELEDVDSEVGCSIKKPKTIMDFVKRSSLEKIISKLEPVDGISIRVISRL*FIRESIYNSGYKLLKNENDVMQLIHDHFEGMKEXXXXXXXKNYK*PEI*LHT**VGQLTNEEIYQC------NSP*CPRSI*KIIGLDRIFR*CTATKLLKVVTYHLGSFKVNYEQHVVGTTGDSASLMVIHGKECPTSYKLCLNHGLHLVVCDTLXXXXXXXXXXXXXXXXXXXXXXTK-------------DSIQKIDC--------IHEIIKNVRKVVKFFRLSPF---ILQEKIRAKLGHNLELYLDCKTRWSSLANMXXXXXXXXXXXXEALVDLNFSHIIDHINFNELKDLQNSLEHAKLAIEVLIGNDTNLHSVRITLEYMKNFMK 2575
            K     T++L  HL+  HDI    ++   E          K   ++++F+KR S+E++++K    DG SI  ++    IRE I    Y + ++E+ + +LI D F   K+   +          +         +T +E   C      N     +     +GL  I   C A ++  +V   L  + +   ++++ +T D AS+M  + +      + C+NH +HL V +T Y K +  +   +  + + + +  +             + I   +         I+EI++N RK V+FFR SP    ILQ  +  K G  L+L LDC+TRW+S+  M+     L+  I+ AL ++    + +  +FN L +L   L+  +LA++ L  + + L +    L ++   MK
Sbjct:   30 KCASGNTSNLANHLRAKHDIDVKKNEEPPEK-------RAKATSSMLNFIKRESVEELLAKCAAYDGFSIDAMTHSSAIREFITTRSYVMPRSESTIKKLIVD-FANEKKLQFKDKFTKMAKSGMCF------SITTDEWTSCSGKRYLNVTLHSQEDSYKLGLIAIECSCDAYQIHSLVEQKLMDYGIKL-RNILTSTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFYNKNNVIDEISDSENADGDEDENQFNSFAGYHNLNIDNDIDNTEYTYSLDDANINEILQNFRKTVRFFRKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNSIIPMMERLVLLQVCIKHALEEIGRDDLYNEKDFNILANLIEILKPTELAVKELSKHTSTLLTAEGVLTFLFTQMK 424

[Close]

BLAST of SMED30012698 vs. Planmine SMEST
Match: SMESG000026901.1 (SMESG000026901.1)

HSP 1 Score: 77.411 bits (189), Expect = 4.036e-14
Identity = 101/404 (25.00%), Postives = 181/404 (44.80%), Query Frame = -3

Query: 1439 TTSLFKHLKT-HDIHPNLDKHELEDVDSEVGCSIKKPKTIMDFVKRSSLEKIISKLEPVDGISIRVISRL*FIRESIYNSGYKLLKNENDVMQLIHDHFEGMKEXXXXXXXKNYK*PEI*LHT**VGQLTNEEIYQC------NSP*CPRSI*KIIGLDRIFR*CTATKLLKVVTYHLGSFKVNYEQHVVGTTGDSASLMVIHGKECPTSYKLCLNHGLHLVVCDTLXXXXXXXXXXXXXXXXXXXXXXTK------DSIQKIDC---------------IHEIIKNVRKVVKFFRLSPF---ILQEKIRAKLGHNLELYLDCKTRWSSLANMXXXXXXXXXXXXEALVDLNFSHIIDHINFNELKDLQNSLEHAKLAIEVLIGNDTNLHSVRITLEYMKNFMK 2557
            T++L  HL+  HDI    ++   E          K    +++F+KR S+ ++++K    DG SI  ++    IRE I    Y + ++E+ + +LI D F   K+   +          I L       +T +E   C      N     +     +GL  I   C A ++  +V   L  + +   ++++ +T D AS+M  + +      + C+NH +HL V +T + K +  +   +  + + + +  +      D    ID                I+EI++N RK V+FFR SP    ILQ  +  K G  L+L LD +TRW+SL  M+     L+  I+ AL ++    + +  +FN L +L   L+  +LA++ L  + + L +    L ++   MK
Sbjct:   36 TSNLANHLRAKHDIDVKKNEEPPEK-------RAKATSNMLNFIKRESVGELLAKCAAYDGFSIHAMTHSSAIREFITTRSYVMPRSESTIKKLIVD-FANEKKIQFKDKFTKMAESGICL------SITTDEWTSCSGKRYLNVTLHSQEDSYKLGLIAIEGSCDAYQIHSLVEQKLMDYGIKL-RNILASTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFHNKNNVIDEISDSENADGDEDENQFNSFAGDHNLNIDNDIDNTEYTYSLDDAKINEILQNFRKTVRFFRKSPVRNDILQRYVEDKEGKKLKLILDSRTRWNSLIPMMERLVLLQVCIKHALEEIGRDDLYNEKDFNILANLIEILKPTELAVKELSKHTSTLLTAEGVLTFLFTQMK 424

The following BLAST results are available for this feature:

BLAST of SMED30012698 vs. Ensembl Human
BLAST of SMED30012698 vs. Ensembl Celegans
BLAST of SMED30012698 vs. Ensembl Fly
BLAST of SMED30012698 vs. Ensembl Zebrafish
BLAST of SMED30012698 vs. Ensembl Xenopus
BLAST of SMED30012698 vs. Ensembl Mouse
BLAST of SMED30012698 vs. UniProt/SwissProt
BLAST of SMED30012698 vs. TrEMBL
BLAST of SMED30012698 vs. Ensembl Cavefish
BLAST of SMED30012698 vs. Ensembl Sea Lamprey
BLAST of SMED30012698 vs. Ensembl Yeast
BLAST of SMED30012698 vs. Ensembl Nematostella
BLAST of SMED30012698 vs. Ensembl Medaka
BLAST of SMED30012698 vs. Planmine SMEST

BLAST of SMED30012698 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30012698 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30012698 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30012698 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30012698 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30012698 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30012698 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30012698 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A4C1X7C4	9.451e-20	33.20	BED-type domain-containing protein OS=Eumeta varie...	[more]
B0W9I7	1.415e-17	28.61	Predicted protein OS=Culex quinquefasciatus OX=717...	[more]
A0A4Y2LA02	1.355e-16	31.85	Uncharacterized protein OS=Araneus ventricosus OX=...	[more]
A0A4C1WY75	4.597e-16	32.81	Uncharacterized protein OS=Eumeta variegata OX=151...	[more]
A0A0N7Z9Z3	5.338e-16	27.86	BED-type domain-containing protein OS=Scylla oliva...	[more]

BLAST of SMED30012698 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30012698 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30012698 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30012698 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30012698 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30012698 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000004539.1	4.799e-23	36.68	SMESG000004539.1	[more]
SMESG000046976.1	2.131e-22	35.65	SMESG000046976.1	[more]
SMESG000068156.1	6.828e-17	36.36	SMESG000068156.1	[more]
SMESG000077975.1	7.087e-15	24.39	SMESG000077975.1	[more]
SMESG000026901.1	4.036e-14	25.00	SMESG000026901.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30012698 ID=SMED30012698|Name=SMED30012698|organism=Schmidtea mediterranea sexual|type=transcript|length=4278bp
TTGCTTAAATCGAATTTATTTAAGGTGGACTTTTAATTTAATTAAGCATT
TCTTATTAAATTTCGCAATTAAAATATCAATTTTATCTTCATTTCCAATA
TAGATCAATGTTTTTTAAAATCCAAATCAATTAAAAAAATATCAAATAAA
ATGTAGACAGAAAACGCACCTTTTCTTAATTTCTGGGAGAACTTAGAAAT
ATTTAGAGGATTTTATTTCAGAATCTTCTTAGTTTAAACCAAGACGCAAC
TTTTTCATGAAGGATGTCTCGACAAATTATGTCGCGTTTTCAATGTTCCC
AAAATGTTCCTTAAAAATAGCAAAATAGTATGAAAAAAAATTTTTTCCTC
GACTTTTTTTTACATATTCTTGTGTGACGTCATCGAACACATCTTTTTCT
TGTAGGATCTCTCGACAAAATATTATGAATAACCAAGATTTACTTTTGAC
TATAGTCTTTTTTTGCACTTTTAAATCAATAATTATTAAATTATTTTTGG
TGCACAAACTTAAGTGCACCTTCATATGGTCTGAAAACCAGAAAGTACCT
CTGACTATAGTTAGAAGAGGCACTTTGCACTTTTTTTCACCCAAAAATCA
ATATATGTAGGCAAAAAGGAGCCCATTTTAAAACCGAAAAAAGGAAATAT
TCTAGTTAGTAAATGCACAGCAGAAAGCCCGAAACCGAAGCTCCTTGTAA
GCTCCTTCTAATCCTTGTAAGATCAATAAATCACGCAGGCAAGCAGATGA
TGTATTATATTACTATTGACGCGCTATTGAATTATTAATTGCACTAATGA
TGGCTGGCAAGCATCTAAGTGAAGCAGCTAAACGACGGAAAAAAGATTTA
CCAAACGAACTTTAGAAAGGATGTAAAATTGACTTATTTTTGTTACCGGC
ACAGGCTAAATTCGAAGAAATACAATCTGTTGCTAATGTACTTAATTTAG
AAAATTTGAAGGATCCTCAGGATGATACCAGTGGGATTGAAGTCAGTAAA
CTACAGATAAATACAAATTATCAGAGTAACGGTTTTAAAGAAAATCGTGC
AAGGGGTTCAGAATTTGAAGTTACTCCACTGCATGGAAAGAAGTAGTTAC
ATAGAAAACTTAATTTGCGTTAACCTATACCATTTTCATGTTGAATTTAT
TAACTTGTTATCCACACCATTAAATTTCTAAGCGCTCTGCATTCCTGTAT
CATGGCGTGTAGGGTTTCTAAAAACTCGGGTTTTAAGACTCGACGACTCC
GAGTCCGTTGTCTTTAACAAAAAATATTACAAGAATCTGATAAAATAATA
TTATATGATTTTCAATTCAACTGAAATTTGTGATTTAATGGTCGTGCCAA
GAAATTAAAAGGACAATGTACCCAAATTTATTGATTAATTCATTTATTTT
CAAAACTTAATAAGCTTTCAAATGAAATAGGTAATATTCTTCATAAAATT
CTTCATATACTCCAATGTAATACGAACTGAATGCAGATTCGTATCGTTTC
CGATTAAAACCTCAATTGCTAATTTGGCATGTTCAAGCGAATTCTGGAGA
TCTTTGAGCTCGTTAAAATTGATGTGATCAATAATGTGTGAAAAGTTGAG
ATCCACTAAAGCTTCCTCGATGAATTTTTCTAGTTTAAAAAATATAATTA
TCATATTAGCAAGAGAACTCCAACGAGTTTTACAATCTAAATATAACTCA
AGATTGTGGCCCAGTTTAGCTCTTATTTTTTCTTGTAATATGAAAGGAGA
CAATCTAAAAAACTTAACCACTTTTCGAACATTCTTAATTATTTCATGGA
TACAATCGATTTTTTGAATACTGTCCTTTGTTGTTTCAATTTCAGTTTCA
AAATCAATTAATTCCTCTTCATTTTCGCTGTACGATTTTTTCTTATATAA
AGTATCACATACAACAAGATGTAGTCCATGATTGAGACACAATTTATAAG
ATGTTGGACACTCCTTTCCATGTATCACCATCAAAGAAGCGCTATCTCCT
GTTGTACCAACAACATGTTGCTCATAATTTACTTTAAACGAACCCAAATG
GTAAGTAACCACTTTAAGTAATTTTGTTGCGGTACACTATCTGAATATAC
GATCTAGTCCAATGATTTTTCATATGGATCTGGGGCACTATGGAGAATTA
CATTGATATATTTCCTCGTTCGTAAGCTGGCCCACTCATCAAGTGTGAAG
CTAAATCTCAGGTTATTTGTAATTTTTTCTAATAATTCTAATTATTTTCT
CTTTCATTCCTTCAAAATGGTCATGTATTAATTGCATTACGTCATTTTCA
TTTTTAAGCAACTTATATCCACTATTATAAATAGATTCCCGTATAAATTA
CAATCTGGAAATCACTCTTATTGAAATTCCGTCTACTGGTTCCAATTTCG
AAATAATTTTTTCTAAACTTGAACGTTTTACAAAATCCATAATTGTCTTT
GGTTTTTTAATTGAACATCCTACTTCAGAATCAACATCCTCCAGTTCATG
TTTGTCCAAATTTGGATGAATATCATGAGTTTTAAGGTGCTTGAATAACG
ATGTTGTGATTGAATCAACAGCTTTTTTTCTCCAATTGAGTGAAATATTC
CCAAACTTTTGATGTTTTTTTTGTGACATTGTTTCTAAGCCGAGGATAAA
AATTAATTAAAAATTTATAATAAATTGATTTCAATAATTTGTAATTATCT
TTTTTTCATACATAAAACAGATTCGTCAATTATAATAATTGTCACAAAAA
CAAAATGATATTGAATTATTATGCTATGACTAGGTATAGGTAGCTAAATA
TACTGTAAGGGTAAACGTGTTATGAAAATTCAAAATTCAAAACCAAATTT
TCTACTTTTATTTTATTTTTACTGACTTACTCACCTACACTTACTACACT
CTTACCACGTATCAAATAGATTTGATTTTTATTTTCATTCAATATTGAGT
TTAAAGTCTAATAATCGTTTTATTATTTCTTACATTGAAAAATAAAAGTT
CTGTTGTTCTGCGAATTCGTACAAAGAAATTTGATGCCAAACCAACAATG
TACAATAAATATTAATATAGGCGTAAGCGACAATGAAATATAATAAATCG
ATCGACGCTTTTTTTGTTTAATTTTAAAAATTTGGACTCGTCGTATATTA
GAGTCGAGTTCTGAGTCCGAGTAACTACCCAAATACCCGACGAGTCATTG
TCGAGTAGAGTAGAGTCAAGAAAGCCTAAACAATTATAAGCTTATTTTCA
CTAATTTCTTTACATAAATTTGTTTTCTCGATGTTTCGAATGTTTTCTAT
TCTACTATATTTTTCAAGACGTGATCACGATAACCAAATTGTATTTTAAT
GATCTTCATTTTGAATTAATAGACCTTGCATAATGACATTAAGAAAACTT
CAAACACATGTAACTAACACAACTTTATACATCGCTAAAAATAATAAGTT
TCCAAACACAACACTTTGTCATTAAAATGCTTATTTTATTTGCATCAACT
TAAATCTGTGAAATAAATATCTCCTATGTAAATATCAATAAATCAAAATA
AATGTCTCAGCTCACTGATTCAAATTTGAGTTCTATACTGCGGATTTCAT
AAAGTAAAATTTCATAAAATCGGAAATTAATTAAAAGCTGCTTGCAATTA
CAAAAATATCATTAATGATTACATATTTTAACTAACAGTATGTAAATAGT
ATATTAAGTAGGTTACTATGATTATTGTCAGTATGTGATTTTACATTTTT
CAAATTTTGTTTTTTTGTTAACAATTTGTTTATTTTTTAACAAATTTTGT
CTCGGATTTCTCTTCATCTGTTGGAAATTATCTTGAGACATACCGAACAA
ATTTAATTTTCATGAGGCTTTTGAGACAAAAAAAAGGTTCCCTACTTTTG
TTCAGAACTCTCGTATATTCTACTTTATACAATTTACCACTTGGAACCGG
CTGACAATGCCACCAACTATATTATCAACTTCAGTATGACATTTATTCTA
ATGTAAAATGCTACCGTAAATATAACTAACCATTTTAATGTTTTATAATG
CAATATTTTCTAAATCAGAATCCATGAGTATTTAAGAAAGGTCTCAATTT
ATAAGGGCTCCACAGTGATTTTTTAAAATCTGAATCTCAAATGGAAATCA
AAAAATCAAACATCAAACATGTAGTCGTTACACATCCTAAAATGCTTTAA
TCAGGCATTGTTCAAGATCAACTTATTTTGATTCCAAATGTCTATGTTAC
AGACGAAATTTCATAAATTTTTTTTGAT

protein sequence of SMED30012698-orf-1

>SMED30012698-orf-1 ID=SMED30012698-orf-1|Name=SMED30012698-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=188bp
MVIHGKECPTSYKLCLNHGLHLVVCDTLYKKKSYSENEEELIDFETEIET
TKDSIQKIDCIHEIIKNVRKVVKFFRLSPFILQEKIRAKLGHNLELYLDC
KTRWSSLANMIIIFFKLEKFIEEALVDLNFSHIIDHINFNELKDLQNSLE
HAKLAIEVLIGNDTNLHSVRITLEYMKNFMKNITYFI*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0002056	medial muscle cell

Vocabulary: **INTERPRO**
Term	Definition
IPR012337	RNaseH-like_sf

Vocabulary: **molecular function**
Term	Definition
GO:0003676	nucleic acid binding

Main menu

SMED30012698

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 1

Owlery

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