Coiled-coil domain-containing protein 51

Overview
NameCoiled-coil domain-containing protein 51
Smed IDSMED30012677
Length (bp)968
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Coiled-coil domain-containing protein 51 (SMED30012677) t-SNE clustered cells

Violin plots show distribution of expression levels for Coiled-coil domain-containing protein 51 (SMED30012677) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Coiled-coil domain-containing protein 51 (SMED30012677) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Coiled-coil domain-containing protein 51 (SMED30012677) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30012677

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30012677h1SMcG0013484 dd_Smed_v4_12107_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30012677h1SMcG0013484 dd_Smed_v4_12107_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30012677h1SMcG0013484 dd_Smed_v4_12107_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Human
Match: CCDC51 (coiled-coil domain containing 51 [Source:HGNC Symbol;Acc:HGNC:25714])

HSP 1 Score: 77.7962 bits (190), Expect = 9.688e-16
Identity = 34/89 (38.20%), Postives = 63/89 (70.79%), Query Frame = 1 
Query:  280 EHFRMMN*KYQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVL 546
            ++  +   +++++ +E  LR     AE+ ERE F+  S+ +RESHEKER+++E+TK WSL+GS++GA IG+ G++  + +R +E++ +L
Sbjct:   37 QYLELATLEHRMLQEEKRLRTAYLRAEDSEREKFSLFSAAVRESHEKERTRAERTKNWSLIGSVLGALIGVAGSTYVNRVRLQELKALL 125
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Human
Match: CCDC51 (coiled-coil domain containing 51 [Source:HGNC Symbol;Acc:HGNC:25714])

HSP 1 Score: 77.7962 bits (190), Expect = 9.688e-16
Identity = 34/89 (38.20%), Postives = 63/89 (70.79%), Query Frame = 1 
Query:  280 EHFRMMN*KYQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVL 546
            ++  +   +++++ +E  LR     AE+ ERE F+  S+ +RESHEKER+++E+TK WSL+GS++GA IG+ G++  + +R +E++ +L
Sbjct:   37 QYLELATLEHRMLQEEKRLRTAYLRAEDSEREKFSLFSAAVRESHEKERTRAERTKNWSLIGSVLGALIGVAGSTYVNRVRLQELKALL 125
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Human
Match: CCDC51 (coiled-coil domain containing 51 [Source:HGNC Symbol;Acc:HGNC:25714])

HSP 1 Score: 77.7962 bits (190), Expect = 9.688e-16
Identity = 34/89 (38.20%), Postives = 63/89 (70.79%), Query Frame = 1 
Query:  280 EHFRMMN*KYQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVL 546
            ++  +   +++++ +E  LR     AE+ ERE F+  S+ +RESHEKER+++E+TK WSL+GS++GA IG+ G++  + +R +E++ +L
Sbjct:   37 QYLELATLEHRMLQEEKRLRTAYLRAEDSEREKFSLFSAAVRESHEKERTRAERTKNWSLIGSVLGALIGVAGSTYVNRVRLQELKALL 125
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Human
Match: CCDC51 (coiled-coil domain containing 51 [Source:HGNC Symbol;Acc:HGNC:25714])

HSP 1 Score: 77.7962 bits (190), Expect = 2.335e-15
Identity = 34/89 (38.20%), Postives = 63/89 (70.79%), Query Frame = 1 
Query:  280 EHFRMMN*KYQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVL 546
            ++  +   +++++ +E  LR     AE+ ERE F+  S+ +RESHEKER+++E+TK WSL+GS++GA IG+ G++  + +R +E++ +L
Sbjct:  146 QYLELATLEHRMLQEEKRLRTAYLRAEDSEREKFSLFSAAVRESHEKERTRAERTKNWSLIGSVLGALIGVAGSTYVNRVRLQELKALL 234
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Celegans
Match: ZC477.3 (pep chromosome:WBcel235:IV:7103506:7104400:1 gene:WBGene00022618.1 transcript:ZC477.3c.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZC477.3)

HSP 1 Score: 75.485 bits (184), Expect = 7.756e-16
Identity = 36/88 (40.91%), Postives = 59/88 (67.05%), Query Frame = 1 
Query:  283 HFRMMN*KYQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVL 546
            + +++  ++QII +E EL    E  E  ERE F  LS ++R SHE+ER ++EKTK+W +  S+IGA +GI G SI + +R ++++ +L
Sbjct:  100 YLQLLTEEHQIIKKERELLNSFEGLETAEREAFHQLSMRVRSSHERERERAEKTKWWGVSASLIGALLGIAGTSIGNELRMRKLKEML 187

HSP 2 Score: 67.0106 bits (162), Expect = 7.946e-13
Identity = 32/81 (39.51%), Postives = 54/81 (66.67%), Query Frame = 3 
Query:   27 NNLSRFIKYYEELIGIKELRIVQANVIKTEQEFLQSQTNRRHFQSEINKILVKRNEINGELENTPRGQKKFLQLVTEEHEV 269
              + +++  YEE IG+K +R  Q  V++ E++  ++Q  RR  QSEIN +  +  EI+ +L+ T RG+ K+LQL+TEEH++
Sbjct:   30 GKIDQYVDVYEEFIGVKTVRQAQEEVMRWEKKLSEAQLVRREKQSEINNLQSRLKEIHFDLDRTSRGEDKYLQLLTEEHQI 110
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Celegans
Match: ZC477.3 (pep chromosome:WBcel235:IV:7103492:7105096:1 gene:WBGene00022618.1 transcript:ZC477.3b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZC477.3)

HSP 1 Score: 76.6406 bits (187), Expect = 1.282e-15
Identity = 36/88 (40.91%), Postives = 59/88 (67.05%), Query Frame = 1 
Query:  283 HFRMMN*KYQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVL 546
            + +++  ++QII +E EL    E  E  ERE F  LS ++R SHE+ER ++EKTK+W +  S+IGA +GI G SI + +R ++++ +L
Sbjct:  100 YLQLLTEEHQIIKKERELLNSFEGLETAEREAFHQLSMRVRSSHERERERAEKTKWWGVSASLIGALLGIAGTSIGNELRMRKLKEML 187

HSP 2 Score: 67.0106 bits (162), Expect = 2.217e-12
Identity = 32/85 (37.65%), Postives = 55/85 (64.71%), Query Frame = 3 
Query:   15 SSFNNNLSRFIKYYEELIGIKELRIVQANVIKTEQEFLQSQTNRRHFQSEINKILVKRNEINGELENTPRGQKKFLQLVTEEHEV 269
            +     + +++  YEE IG+K +R  Q  V++ E++  ++Q  RR  QSEIN +  +  EI+ +L+ T RG+ K+LQL+TEEH++
Sbjct:   26 TKVRGKIDQYVDVYEEFIGVKTVRQAQEEVMRWEKKLSEAQLVRREKQSEINNLQSRLKEIHFDLDRTSRGEDKYLQLLTEEHQI 110
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Celegans
Match: ZC477.3 (pep chromosome:WBcel235:IV:7103492:7105096:1 gene:WBGene00022618.1 transcript:ZC477.3a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZC477.3)

HSP 1 Score: 76.6406 bits (187), Expect = 1.415e-15
Identity = 36/88 (40.91%), Postives = 59/88 (67.05%), Query Frame = 1 
Query:  283 HFRMMN*KYQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVL 546
            + +++  ++QII +E EL    E  E  ERE F  LS ++R SHE+ER ++EKTK+W +  S+IGA +GI G SI + +R ++++ +L
Sbjct:  100 YLQLLTEEHQIIKKERELLNSFEGLETAEREAFHQLSMRVRSSHERERERAEKTKWWGVSASLIGALLGIAGTSIGNELRMRKLKEML 187

HSP 2 Score: 67.0106 bits (162), Expect = 2.343e-12
Identity = 32/85 (37.65%), Postives = 55/85 (64.71%), Query Frame = 3 
Query:   15 SSFNNNLSRFIKYYEELIGIKELRIVQANVIKTEQEFLQSQTNRRHFQSEINKILVKRNEINGELENTPRGQKKFLQLVTEEHEV 269
            +     + +++  YEE IG+K +R  Q  V++ E++  ++Q  RR  QSEIN +  +  EI+ +L+ T RG+ K+LQL+TEEH++
Sbjct:   26 TKVRGKIDQYVDVYEEFIGVKTVRQAQEEVMRWEKKLSEAQLVRREKQSEINNLQSRLKEIHFDLDRTSRGEDKYLQLLTEEHQI 110
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Zebrafish
Match: ccdc51 (coiled-coil domain containing 51 [Source:ZFIN;Acc:ZDB-GENE-060825-200])

HSP 1 Score: 78.1814 bits (191), Expect = 1.223e-15
Identity = 34/81 (41.98%), Postives = 61/81 (75.31%), Query Frame = 1 
Query:  304 KYQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVL 546
            +++++ +E  LR   E AE  ERE FA  S+ +RESH+KER+++E+TK WS++GS++GA IG++G++  + +R +E++ +L
Sbjct:  157 EHKLLQEERRLRTSHENAEEAEREKFALFSAAVRESHDKERTRAERTKNWSIIGSVLGAIIGVMGSTYINRVRLQELKSLL 237
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Zebrafish
Match: ccdc51 (coiled-coil domain containing 51 [Source:ZFIN;Acc:ZDB-GENE-060825-200])

HSP 1 Score: 78.1814 bits (191), Expect = 1.223e-15
Identity = 34/81 (41.98%), Postives = 61/81 (75.31%), Query Frame = 1 
Query:  304 KYQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVL 546
            +++++ +E  LR   E AE  ERE FA  S+ +RESH+KER+++E+TK WS++GS++GA IG++G++  + +R +E++ +L
Sbjct:  157 EHKLLQEERRLRTSHENAEEAEREKFALFSAAVRESHDKERTRAERTKNWSIIGSVLGAIIGVMGSTYINRVRLQELKSLL 237
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Xenopus
Match: snapc5 (small nuclear RNA activating complex polypeptide 5 [Source:Xenbase;Acc:XB-GENE-973982])

HSP 1 Score: 75.8702 bits (185), Expect = 8.490e-15
Identity = 34/89 (38.20%), Postives = 63/89 (70.79%), Query Frame = 1 
Query:  280 EHFRMMN*KYQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVL 546
            ++  +   +++++ +E  LRA    AE  ERE F+  S+ +R SHEKER+++E+TK WS++GS++GA IG++G++  + IR +E++ +L
Sbjct:  147 QYLELATLEHKLLQEEKRLRALYVNAEESEREKFSLFSASVRASHEKERTRAERTKNWSIIGSVLGALIGVMGSTYINRIRLQELKSLL 235
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Mouse
Match: Ccdc51 (coiled-coil domain containing 51 [Source:MGI Symbol;Acc:MGI:1913908])

HSP 1 Score: 77.0258 bits (188), Expect = 2.626e-15
Identity = 34/89 (38.20%), Postives = 63/89 (70.79%), Query Frame = 1 
Query:  280 EHFRMMN*KYQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVL 546
            ++  +   +++++ +E  LR     AE+ ERE F+  S+ +RESHEKER+++E+TK WSL+GS++GA IG+ G++  + +R +E++ +L
Sbjct:  143 QYLELATLEHRMLQEEKRLRIAYLRAEDSEREKFSLFSAAVRESHEKERTRAERTKNWSLIGSVLGALIGVAGSTYVNRVRLQELKALL 231
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. UniProt/SwissProt
Match: sp|Q96ER9|MITOK_HUMAN (Mitochondrial potassium channel OS=Homo sapiens OX=9606 GN=CCDC51 PE=1 SV=2)

HSP 1 Score: 77.7962 bits (190), Expect = 1.121e-14
Identity = 34/89 (38.20%), Postives = 63/89 (70.79%), Query Frame = 1 
Query:  280 EHFRMMN*KYQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVL 546
            ++  +   +++++ +E  LR     AE+ ERE F+  S+ +RESHEKER+++E+TK WSL+GS++GA IG+ G++  + +R +E++ +L
Sbjct:  146 QYLELATLEHRMLQEEKRLRTAYLRAEDSEREKFSLFSAAVRESHEKERTRAERTKNWSLIGSVLGALIGVAGSTYVNRVRLQELKALL 234
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. UniProt/SwissProt
Match: sp|Q3URS9|MITOK_MOUSE (Mitochondrial potassium channel OS=Mus musculus OX=10090 GN=Ccdc51 PE=1 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.837e-14
Identity = 34/89 (38.20%), Postives = 63/89 (70.79%), Query Frame = 1 
Query:  280 EHFRMMN*KYQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVL 546
            ++  +   +++++ +E  LR     AE+ ERE F+  S+ +RESHEKER+++E+TK WSL+GS++GA IG+ G++  + +R +E++ +L
Sbjct:  143 QYLELATLEHRMLQEEKRLRIAYLRAEDSEREKFSLFSAAVRESHEKERTRAERTKNWSLIGSVLGALIGVAGSTYVNRVRLQELKALL 231
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. UniProt/SwissProt
Match: sp|Q5PPN7|MITOK_RAT (Mitochondrial potassium channel OS=Rattus norvegicus OX=10116 GN=Ccdc51 PE=2 SV=2)

HSP 1 Score: 77.0258 bits (188), Expect = 1.850e-14
Identity = 34/89 (38.20%), Postives = 63/89 (70.79%), Query Frame = 1 
Query:  280 EHFRMMN*KYQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVL 546
            ++  +   +++++ +E  LR     AE+ ERE F+  S+ +RESHEKER+++E+TK WSL+GS++GA IG+ G++  + +R +E++ +L
Sbjct:  146 QYLELATLEHRMLQEEKRLRIAYLRAEDSEREKFSLFSAAVRESHEKERTRAERTKNWSLIGSVLGALIGVAGSTYVNRVRLQELKALL 234
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. UniProt/SwissProt
Match: sp|Q5E9Q3|MITOK_BOVIN (Mitochondrial potassium channel OS=Bos taurus OX=9913 GN=CCDC51 PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 2.457e-14
Identity = 34/89 (38.20%), Postives = 63/89 (70.79%), Query Frame = 1 
Query:  280 EHFRMMN*KYQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVL 546
            ++  +   +++++ +E  LR     AE+ ERE F+  S+ +RESHEKER+++E+TK WSL+GS++GA IG+ G++  + +R +E++ +L
Sbjct:  141 QYLELATLEHRMLQEEKRLRMTYLRAEDSEREKFSLFSAAVRESHEKERTRAERTKNWSLIGSVLGALIGVAGSTYVNRVRLQELKALL 229
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. TrEMBL
Match: A0A553PBQ8 (Uncharacterized protein OS=Tigriopus californicus OX=6832 GN=TCAL_08496 PE=4 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 5.329e-29
Identity = 38/82 (46.34%), Postives = 60/82 (73.17%), Query Frame = 1 
Query:  307 YQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVLHR 552
            +Q++ +E  L       E  ERE FA LS+ +R+SHEKER+Q+EKTKYWS++GSI+G  +GI+G +IN+ +R +E+R ++ +
Sbjct:  183 HQVLKEEKALLEDFTQLEKRERECFALLSNAVRDSHEKERAQAEKTKYWSVLGSILGTCLGIIGTTINNRLRMRELRELVAK 264

HSP 2 Score: 70.8626 bits (172), Expect = 5.329e-29
Identity = 33/76 (43.42%), Postives = 53/76 (69.74%), Query Frame = 3 
Query:   42 FIKYYEELIGIKELRIVQANVIKTEQEFLQSQTNRRHFQSEINKILVKRNEINGELENTPRGQKKFLQLVTEEHEV 269
            +++ YE+ +G+ E++  Q  V++ E+ F++ Q +RR  QS+I  I  K  EI+GELE T RG+ K+L LVT+EH+V
Sbjct:  110 WMRQYEDFVGLTEVKQAQGKVVELERRFIEQQDSRREAQSDITSIQSKIKEIHGELEKTYRGEDKYLTLVTQEHQV 185
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. TrEMBL
Match: A0A1W0XAM5 (Putative Coiled-coil domain-containing protein 51 OS=Hypsibius dujardini OX=232323 GN=BV898_01907 PE=4 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 2.636e-27
Identity = 42/87 (48.28%), Postives = 66/87 (75.86%), Query Frame = 1 
Query:  313 IITQENEL----RAKLEAA---ENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVLHR 552
            ++TQE++L    R   +AA   E  ER  F+ LS+ +RESHEKER+++E+TKYWS++GS+IGA IGI+G S+N+++R KE++ ++ R
Sbjct:   95 LVTQEHKLVKEERTFQQAANDSETAERGAFSLLSAAVRESHEKERARAERTKYWSVIGSVIGAMIGIVGTSLNNHLRMKELQSLVTR 181

HSP 2 Score: 66.6254 bits (161), Expect = 2.636e-27
Identity = 29/83 (34.94%), Postives = 56/83 (67.47%), Query Frame = 3 
Query:   21 FNNNLSRFIKYYEELIGIKELRIVQANVIKTEQEFLQSQTNRRHFQSEINKILVKRNEINGELENTPRGQKKFLQLVTEEHEV 269
            +N  ++ +I+ YE+ +G+ E++  Q++V++ E+ F+QSQ  RR    +++ +  K  +I  ELE T RG+ ++L LVT+EH++
Sbjct:   20 YNQKITSWIQQYEDFLGLTEVKEAQSSVMEAEKSFIQSQQVRRERNRKVSDVQQKLQDIRAELERTARGEDRYLALVTQEHKL 102
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. TrEMBL
Match: A0A1B6LD19 (Uncharacterized protein OS=Graphocephala atropunctata OX=36148 GN=g.15999 PE=4 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 4.958e-27
Identity = 38/80 (47.50%), Postives = 63/80 (78.75%), Query Frame = 1 
Query:  307 YQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVL 546
            + ++ +E +L A+   +E  ERE F +LSS L+ESHEKER+Q+E+TKYWS++GS+IG  IG++G+SIN+  + +E+R+++
Sbjct:  149 HNLLKEERKLTAQFNLSEQEERECFTFLSSSLKESHEKERAQAERTKYWSVIGSVIGTVIGVVGSSINNEFKMRELRKLV 228

HSP 2 Score: 59.3066 bits (142), Expect = 4.958e-27
Identity = 36/98 (36.73%), Postives = 55/98 (56.12%), Query Frame = 3 
Query:    3 HKFYSS---FNNNLSRFIKY--------YEELIGIKELRIVQANVIKTEQEFLQSQTNRRHFQSEINKILVKRNEINGELENTPRGQKKFLQLVTEEH 263
            H   SS   F+++ + FIK         YE++ G+ E+R  Q  VI+ + +F+ +Q  RR    E+  I  K  E+ GELE T RG++++L LVT EH
Sbjct:   52 HSLTSSKFIFDSSETNFIKKKIDDLVTKYEQITGMDEVRKAQNRVIEAQDKFIIAQEKRRDLVKELTSIQNKLKEVYGELEATTRGEERYLTLVTREH 149
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. TrEMBL
Match: A0A1D1UV63 (Uncharacterized protein OS=Ramazzottius varieornatus OX=947166 GN=RvY_05475-1 PE=4 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 9.207e-27
Identity = 37/81 (45.68%), Postives = 64/81 (79.01%), Query Frame = 1 
Query:  304 KYQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVL 546
            ++++  +E  LR  +   EN ER+TFA LS+ +R+SH+KER+++E+TKYWS++GS++GA IGILG S+N+ ++ +E++ +L
Sbjct:  142 EHKLAKEERVLRQDVMELENTERDTFALLSAAVRDSHDKERARAERTKYWSVMGSVVGALIGILGTSLNNRLKMRELQFML 222

HSP 2 Score: 65.4698 bits (158), Expect = 9.207e-27
Identity = 32/88 (36.36%), Postives = 54/88 (61.36%), Query Frame = 3 
Query:    6 KFYSSFNNNLSRFIKYYEELIGIKELRIVQANVIKTEQEFLQSQTNRRHFQSEINKILVKRNEINGELENTPRGQKKFLQLVTEEHEV 269
            K+ +     +S  I+ YE+ IG+ E++  Q  VI+ E+ F+ +Q  RR    ++ +I  K  E+  ELE TPRG+ ++L LVT+EH++
Sbjct:   58 KWIAPLQQKISSTIRAYEDFIGLTEVKEAQEKVIQGERNFVMAQRARRERNRQMLEIHSKMKEVVAELERTPRGEDRYLTLVTQEHKL 145
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. TrEMBL
Match: A0A085MC64 (Uncharacterized protein OS=Trichuris suis OX=68888 GN=M513_04244 PE=4 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.028e-26
Identity = 36/81 (44.44%), Postives = 58/81 (71.60%), Query Frame = 1 
Query:  304 KYQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVL 546
            ++ ++  E  +   L   E  ERE F+ LS+ LRESHEKER + E+TKYWS++GS++GA IGI+G ++N+  R +E++R++
Sbjct:   98 EHSVLKSERAITTGLHEVEMCEREAFSVLSACLRESHEKERVRVERTKYWSIIGSMVGALIGIIGTTVNNERRMRELKRIV 178

HSP 2 Score: 67.781 bits (164), Expect = 1.028e-26
Identity = 35/85 (41.18%), Postives = 50/85 (58.82%), Query Frame = 3 
Query:   15 SSFNNNLSRFIKYYEELIGIKELRIVQANVIKTEQEFLQSQTNRRHFQSEINKILVKRNEINGELENTPRGQKKFLQLVTEEHEV 269
            SS  + + R +K YE  +G+ E+R+ Q  V++ E+ F   Q  RR  QSE+ KI  +  E+   LE TPR   +FL L+TEEH V
Sbjct:   17 SSVWSAIDRIVKRYENFVGLTEVRLAQEKVLEAEKVFTAKQRLRRQSQSELQKIQERHRELQLALERTPRTDDRFLSLLTEEHSV 101
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Cavefish
Match: ccdc51 (coiled-coil domain containing 51 [Source:NCBI gene;Acc:103044947])

HSP 1 Score: 78.9518 bits (193), Expect = 4.802e-16
Identity = 35/80 (43.75%), Postives = 60/80 (75.00%), Query Frame = 1 
Query:  307 YQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVL 546
            ++++ +E  L+   E AE  ERE FA  S+ +RESHEKERS++E+TK WS++GS++GA IG++G++  + +R +E++ +L
Sbjct:  159 HKLLQEERRLKTTYENAEECEREKFALFSAGVRESHEKERSRAERTKNWSVIGSVLGAIIGVMGSTYVNRVRLQELKGLL 238
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Nematostella
Match: EDO43887 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXR3])

HSP 1 Score: 69.3218 bits (168), Expect = 2.832e-21
Identity = 30/61 (49.18%), Postives = 48/61 (78.69%), Query Frame = 1 
Query:  307 YQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIG 489
            + I+ +E +L+   E  EN ER+ FA LS+ +R+SHEKER+++E+TKYWS++GS+ GA++G
Sbjct:   79 HGILREEKKLKLDFEEMENLERDHFASLSAVVRDSHEKERARAERTKYWSVLGSVAGASLG 139

HSP 2 Score: 50.0618 bits (118), Expect = 2.832e-21
Identity = 23/72 (31.94%), Postives = 45/72 (62.50%), Query Frame = 3 
Query:   54 YEELIGIKELRIVQANVIKTEQEFLQSQTNRRHFQSEINKILVKRNEINGELENTPRGQKKFLQLVTEEHEV 269
            +++++GI E+R  Q NV + E EF++ +   +  + E++ I  + +E+   L+ TPR  +++L L TEEH +
Sbjct:   10 FDDVLGISEVRNAQENVRRAEDEFMECRKRVQTAKKELDFIHSQLSEVRRRLDRTPRDDERYLTLATEEHGI 81
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Medaka
Match: ccdc51 (coiled-coil domain containing 51 [Source:NCBI gene;Acc:101174163])

HSP 1 Score: 79.337 bits (194), Expect = 3.742e-16
Identity = 34/81 (41.98%), Postives = 62/81 (76.54%), Query Frame = 1 
Query:  304 KYQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVL 546
            +++++ +E  LR   E+AE  ERE F+  S+ +RESHEKER+++E+TK WS++GS++GA IG++G++  + +R +E++ +L
Sbjct:  161 EHKLLQEERRLRTAYESAEGSEREKFSLFSAAVRESHEKERTRAERTKNWSVIGSVLGALIGVMGSTYINRVRLQELKNLL 241
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. Planmine SMEST
Match: SMESG000058452.1 (SMESG000058452.1)

HSP 1 Score: 377.096 bits (967), Expect = 9.066e-152
Identity = 199/203 (98.03%), Postives = 203/203 (100.00%), Query Frame = 1 
Query:  304 KYQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVLHRMEFMXXXXXXXXXXXXXDKQNHLMAILTNVNENLENFHKEFPTPINSFGNKLETLNLTLGKLEKLQLIPINTLEPNQDFIDTNDNEKLRHIVVGSTSLLCFSLAFTILYLTFSSASDALF 912
            +++IITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVLHRMEFMNNTNSVSNANNLSDKQNHLMAILTNVNENLENFHKEFPTPINSFGNKLETLNLTLGKLEKLQL+PINTLEPNQDFIDTNDNEKLRHIVVGSTSLLCFSLAFTILYLTFSSASDALF
Sbjct:   87 EHEIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVLHRMEFMNNTNSVSNANNLSDKQNHLMAILTNVNENLENFHKEFPTPINSFGNKLETLNLTLGKLEKLQLMPINTLEPNQDFIDTNDNEKLRHIVVGSTSLLCFSLAFTILYLTFSSASDALF 289

HSP 2 Score: 179.489 bits (454), Expect = 9.066e-152
Identity = 88/89 (98.88%), Postives = 89/89 (100.00%), Query Frame = 3 
Query:    3 HKFYSSFNNNLSRFIKYYEELIGIKELRIVQANVIKTEQEFLQSQTNRRHFQSEINKILVKRNEINGELENTPRGQKKFLQLVTEEHEV 269
            HKFYSSFNNNLSRFIKYYEELIGIKELRIVQANVIKTEQEFLQSQTNRRHFQSEINKILVKRNEINGELENTPRGQKKFLQLVTEEHE+
Sbjct:    2 HKFYSSFNNNLSRFIKYYEELIGIKELRIVQANVIKTEQEFLQSQTNRRHFQSEINKILVKRNEINGELENTPRGQKKFLQLVTEEHEI 90
[Close]
BLAST of Coiled-coil domain-containing protein 51 vs. Planmine SMEST
Match: SMESG000058452.1 (SMESG000058452.1)

HSP 1 Score: 328.176 bits (840), Expect = 4.686e-137
Identity = 180/203 (88.67%), Postives = 184/203 (90.64%), Query Frame = 1 
Query:  304 KYQIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVLHRMEFMXXXXXXXXXXXXXDKQNHLMAILTNVNENLENFHKEFPTPINSFGNKLETLNLTLGKLEKLQLIPINTLEPNQDFIDTNDNEKLRHIVVGSTSLLCFSLAFTILYLTFSSASDALF 912
            +++IITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVLHRMEFMNNTNSVSNANNLSDKQ                   EFPTPINSFGNKLETLNLTLGKLEKLQL+PINTLEPNQDFIDTNDNEKLRHIVVGSTSLLCFSLAFTILYLTFSSASDALF
Sbjct:   87 EHEIITQENELRAKLEAAENHERETFAYLSSKLRESHEKERSQSEKTKYWSLVGSIIGATIGILGASINSYIRNKEIRRVLHRMEFMNNTNSVSNANNLSDKQ-------------------EFPTPINSFGNKLETLNLTLGKLEKLQLMPINTLEPNQDFIDTNDNEKLRHIVVGSTSLLCFSLAFTILYLTFSSASDALF 270

HSP 2 Score: 179.489 bits (454), Expect = 4.686e-137
Identity = 88/89 (98.88%), Postives = 89/89 (100.00%), Query Frame = 3 
Query:    3 HKFYSSFNNNLSRFIKYYEELIGIKELRIVQANVIKTEQEFLQSQTNRRHFQSEINKILVKRNEINGELENTPRGQKKFLQLVTEEHEV 269
            HKFYSSFNNNLSRFIKYYEELIGIKELRIVQANVIKTEQEFLQSQTNRRHFQSEINKILVKRNEINGELENTPRGQKKFLQLVTEEHE+
Sbjct:    2 HKFYSSFNNNLSRFIKYYEELIGIKELRIVQANVIKTEQEFLQSQTNRRHFQSEINKILVKRNEINGELENTPRGQKKFLQLVTEEHEI 90
The following BLAST results are available for this feature:
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
CCDC519.688e-1638.20coiled-coil domain containing 51 [Source:HGNC Symb... [more]
CCDC519.688e-1638.20coiled-coil domain containing 51 [Source:HGNC Symb... [more]
CCDC519.688e-1638.20coiled-coil domain containing 51 [Source:HGNC Symb... [more]
CCDC512.335e-1538.20coiled-coil domain containing 51 [Source:HGNC Symb... [more]
back to top
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ZC477.37.756e-1640.91pep chromosome:WBcel235:IV:7103506:7104400:1 gene:... [more]
ZC477.31.282e-1540.91pep chromosome:WBcel235:IV:7103492:7105096:1 gene:... [more]
ZC477.31.415e-1540.91pep chromosome:WBcel235:IV:7103492:7105096:1 gene:... [more]
back to top
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ccdc511.223e-1541.98coiled-coil domain containing 51 [Source:ZFIN;Acc:... [more]
ccdc511.223e-1541.98coiled-coil domain containing 51 [Source:ZFIN;Acc:... [more]
back to top
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
snapc58.490e-1538.20small nuclear RNA activating complex polypeptide 5... [more]
back to top
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Ccdc512.626e-1538.20coiled-coil domain containing 51 [Source:MGI Symbo... [more]
back to top
BLAST of Coiled-coil domain-containing protein 51 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q96ER9|MITOK_HUMAN1.121e-1438.20Mitochondrial potassium channel OS=Homo sapiens OX... [more]
sp|Q3URS9|MITOK_MOUSE1.837e-1438.20Mitochondrial potassium channel OS=Mus musculus OX... [more]
sp|Q5PPN7|MITOK_RAT1.850e-1438.20Mitochondrial potassium channel OS=Rattus norvegic... [more]
sp|Q5E9Q3|MITOK_BOVIN2.457e-1438.20Mitochondrial potassium channel OS=Bos taurus OX=9... [more]
back to top
BLAST of Coiled-coil domain-containing protein 51 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A553PBQ85.329e-2946.34Uncharacterized protein OS=Tigriopus californicus ... [more]
A0A1W0XAM52.636e-2748.28Putative Coiled-coil domain-containing protein 51 ... [more]
A0A1B6LD194.958e-2747.50Uncharacterized protein OS=Graphocephala atropunct... [more]
A0A1D1UV639.207e-2745.68Uncharacterized protein OS=Ramazzottius varieornat... [more]
A0A085MC641.028e-2644.44Uncharacterized protein OS=Trichuris suis OX=68888... [more]
back to top
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ccdc514.802e-1643.75coiled-coil domain containing 51 [Source:NCBI gene... [more]
back to top
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO438872.832e-2149.18Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of Coiled-coil domain-containing protein 51 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ccdc513.742e-1641.98coiled-coil domain containing 51 [Source:NCBI gene... [more]
back to top
BLAST of Coiled-coil domain-containing protein 51 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000058452.19.066e-15298.03SMESG000058452.1[more]
SMESG000058452.14.686e-13788.67SMESG000058452.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012677 ID=SMED30012677|Name=Coiled-coil domain-containing protein 51|organism=Schmidtea mediterranea sexual|type=transcript|length=968bp
TGCATAAATTTTATTCTTCTTTTAACAATAATCTTTCTAGATTTATTAAA
TATTACGAGGAATTAATTGGAATTAAAGAATTACGCATTGTTCAGGCTAA
TGTTATTAAAACCGAACAAGAATTTTTACAGTCACAAACTAATCGTAGAC
ATTTTCAATCCGAGATCAATAAAATTCTTGTTAAGCGTAATGAGATAAAT
GGGGAATTAGAAAATACTCCAAGAGGACAAAAAAAATTTTTGCAATTAGT
GACTGAAGAGCACGAAGTAAATATAATAGGAACACTTTCGGATGATGAAT
TAAAAATATCAGATTATCACACAAGAAAATGAATTGAGAGCTAAACTGGA
AGCTGCTGAAAACCATGAAAGAGAAACCTTCGCCTATTTATCATCAAAGT
TAAGAGAATCTCACGAAAAAGAAAGATCACAATCAGAAAAAACAAAATAT
TGGTCTCTAGTCGGATCTATTATTGGGGCAACAATCGGAATTTTGGGAGC
TTCTATTAATAGTTATATTAGAAATAAAGAAATTCGGCGAGTTTTGCATC
GGATGGAATTCATGAACAATACAAATTCAGTTTCTAATGCAAATAATTTA
TCAGATAAACAAAATCATTTGATGGCTATATTGACCAATGTTAATGAAAA
TCTCGAAAACTTTCACAAGGAATTTCCAACACCAATCAATTCCTTTGGAA
ACAAATTAGAGACATTAAATTTAACATTGGGGAAACTGGAAAAATTACAG
TTAATACCGATTAATACATTGGAACCAAATCAGGATTTTATTGATACAAA
TGACAATGAGAAACTGCGCCACATCGTCGTTGGTTCTACATCACTGTTAT
GTTTTTCTTTGGCATTTACGATTTTATATTTGACATTTTCCAGTGCTTCC
GATGCGCTGTTTTGAACTTAAACTTAATGATTTTATTTTTGACAAATATA
TTTCTATGTCTAAAAAAA
back to top

protein sequence of SMED30012677-orf-1

>SMED30012677-orf-1 ID=SMED30012677-orf-1|Name=SMED30012677-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=121bp
MEFMNNTNSVSNANNLSDKQNHLMAILTNVNENLENFHKEFPTPINSFGN
KLETLNLTLGKLEKLQLIPINTLEPNQDFIDTNDNEKLRHIVVGSTSLLC
FSLAFTILYLTFSSASDALF*
back to top

protein sequence of SMED30012677-orf-2

>SMED30012677-orf-2 ID=SMED30012677-orf-2|Name=SMED30012677-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=110bp
HKFYSSFNNNLSRFIKYYEELIGIKELRIVQANVIKTEQEFLQSQTNRRH
FQSEINKILVKRNEINGELENTPRGQKKFLQLVTEEHEVNIIGTLSDDEL
KISDYHTRK*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000043dorsal ventral margin of the whole animal
PLANA:0000070intestinal phagocyte
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 91..113