NPHS1-6
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30012656 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Homology
BLAST of NPHS1-6 vs. Ensembl Human
Match: NPHS1 (NPHS1 adhesion molecule, nephrin [Source:HGNC Symbol;Acc:HGNC:7908]) HSP 1 Score: 109.768 bits (273), Expect = 2.039e-23 Identity = 150/683 (21.96%), Postives = 276/683 (40.41%), Query Frame = 2
Query: 218 QCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKP---VCSAVKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHP-TGSVSSNGVQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRGKDLRSVEK--IWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMF---FMDQFWLKSTVTIMKIKRP-ERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNT-------VILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFIN 2215
+ F +P VVE ++E+ C +S VQW + LLG D I G PRYR+ GDP++GE L I+ + DD +YECQV G ++ I+ + PKL L+ + + T + + C+S +P ++G+ + + V + L + P + Q + C ++ A P + V + PP P I+ + + +S++L C G P A+L W N + T T++ + S+ + + + H L C A + + +G+ + + +PP I I L S + +N +NV ++C++ V L+W + + L +E+ + + + S+++ + + L C F F + + KS I+ +K P ++L+IE L ++L CL+ GG P+P L W + T+ +S L + + V V++ +T V + + + S +L V FPP + I N + L + + CV+ SNPP L W + + E + + + F G+ +S+L L ++ H + C ++S + S +L + ++P F+
Sbjct: 32 RGFWALPENLTVVE-GASVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQV---GRSEMGPELVSPRVILSILVPPKLLL----LTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPALE----APIKASFTVNVLFPPGPPVIE-WPGLDEGHVRAGQSLELPC-VARGGNPLATLQW---LKNGQPVSTAWG--------TEHTQAVARSVLVMTVRPEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIII------LGSASQTEN-----KNVT--LSCVSKSSRPRVLLRWWLG----WRQLLPMEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAFSEAFTKETFKKS--LILNVKYPAQKLWIEGPPEGQKLRAGTRVRLVCLAIGGNPEPSLMWYKDSRTVTESR---LPQESRRVHLG-SVEKSGSTFSRELVLVTGPSDNQAKFTCKAGQLSASTQLAVQFPPTNVTILANA-SALRPGDALNLTCVSVSSNPPVNLSWDKEGERL-------EGVAAPPRRAPFKGSA----AARSVL-LQVSSRDHGQRVTCR---AHSAELRETVSSFYRLNVLYRPEFLG 650
BLAST of NPHS1-6 vs. Ensembl Human
Match: NPHS1 (NPHS1 adhesion molecule, nephrin [Source:HGNC Symbol;Acc:HGNC:7908]) HSP 1 Score: 109.768 bits (273), Expect = 2.210e-23 Identity = 150/683 (21.96%), Postives = 276/683 (40.41%), Query Frame = 2
Query: 218 QCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKP---VCSAVKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHP-TGSVSSNGVQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRGKDLRSVEK--IWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMF---FMDQFWLKSTVTIMKIKRP-ERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNT-------VILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFIN 2215
+ F +P VVE ++E+ C +S VQW + LLG D I G PRYR+ GDP++GE L I+ + DD +YECQV G ++ I+ + PKL L+ + + T + + C+S +P ++G+ + + V + L + P + Q + C ++ A P + V + PP P I+ + + +S++L C G P A+L W N + T T++ + S+ + + + H L C A + + +G+ + + +PP I I L S + +N +NV ++C++ V L+W + + L +E+ + + + S+++ + + L C F F + + KS I+ +K P ++L+IE L ++L CL+ GG P+P L W + T+ +S L + + V V++ +T V + + + S +L V FPP + I N + L + + CV+ SNPP L W + + E + + + F G+ +S+L L ++ H + C ++S + S +L + ++P F+
Sbjct: 32 RGFWALPENLTVVE-GASVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQV---GRSEMGPELVSPRVILSILVPPKLLL----LTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPALE----APIKASFTVNVLFPPGPPVIE-WPGLDEGHVRAGQSLELPC-VARGGNPLATLQW---LKNGQPVSTAWG--------TEHTQAVARSVLVMTVRPEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIII------LGSASQTEN-----KNVT--LSCVSKSSRPRVLLRWWLG----WRQLLPMEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAFSEAFTKETFKKS--LILNVKYPAQKLWIEGPPEGQKLRAGTRVRLVCLAIGGNPEPSLMWYKDSRTVTESR---LPQESRRVHLG-SVEKSGSTFSRELVLVTGPSDNQAKFTCKAGQLSASTQLAVQFPPTNVTILANA-SALRPGDALNLTCVSVSSNPPVNLSWDKEGERL-------EGVAAPPRRAPFKGSA----AARSVL-LQVSSRDHGQRVTCR---AHSAELRETVSSFYRLNVLYRPEFLG 650
BLAST of NPHS1-6 vs. Ensembl Human
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:HGNC Symbol;Acc:HGNC:23204]) HSP 1 Score: 88.5817 bits (218), Expect = 5.240e-17 Identity = 76/260 (29.23%), Postives = 116/260 (44.62%), Query Frame = 2
Query: 245 EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSA------VKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWS 1006
+ VV Q + + C I G V W + LG R + P+Y +VG+ GEH L+I ++DD YECQ I+ R LT++V P+ P + L ++ L D P+ LTC + + +P S W G+ + A ++ + ES+ VS L P +++ QSI C TN A P ++ + IQ PP V S + VLE +V + HC+ PA + Y RW+
Sbjct: 56 DQVVVSGQPVTLLCAIPEYDGFVLWIKDGLALGVGRDLSSYPQYLVVGNHLSGEHHLKILRAELQDDAVYECQAIQAAIRS----RPARLTVLVPPDDPVI------LGGPVISLRAGD---PLNLTCHADNAKPAASIIWLRKGEVINGATYSKTLLRDGKRESI---VSTLFISPGDVENGQSIVCRATNKA---IPG-GKETSVTIDIQHPPLVN--LSVEPQPVLEDNV-----VTFHCSAKANPAVTQY---RWA 285
BLAST of NPHS1-6 vs. Ensembl Human
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:HGNC Symbol;Acc:HGNC:23204]) HSP 1 Score: 88.5817 bits (218), Expect = 5.342e-17 Identity = 76/260 (29.23%), Postives = 116/260 (44.62%), Query Frame = 2
Query: 245 EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSA------VKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWS 1006
+ VV Q + + C I G V W + LG R + P+Y +VG+ GEH L+I ++DD YECQ I+ R LT++V P+ P + L ++ L D P+ LTC + + +P S W G+ + A ++ + ES+ VS L P +++ QSI C TN A P ++ + IQ PP V S + VLE +V + HC+ PA + Y RW+
Sbjct: 56 DQVVVSGQPVTLLCAIPEYDGFVLWIKDGLALGVGRDLSSYPQYLVVGNHLSGEHHLKILRAELQDDAVYECQAIQAAIRS----RPARLTVLVPPDDPVI------LGGPVISLRAGD---PLNLTCHADNAKPAASIIWLRKGEVINGATYSKTLLRDGKRESI---VSTLFISPGDVENGQSIVCRATNKAI---PG-GKETSVTIDIQHPPLVN--LSVEPQPVLEDNV-----VTFHCSAKANPAVTQY---RWA 285
BLAST of NPHS1-6 vs. Ensembl Human
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:HGNC Symbol;Acc:HGNC:23204]) HSP 1 Score: 87.4261 bits (215), Expect = 7.171e-17 Identity = 76/262 (29.01%), Postives = 117/262 (44.66%), Query Frame = 2
Query: 245 EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSA------VKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQ--TLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWS 1006
+ VV Q + + C I G V W + LG R + P+Y +VG+ GEH L+I ++DD YECQ I+ R LT++V P+ P + L ++ L D P+ LTC + + +P S W G+ + A ++ + ES+ VS L P +++ QSI C TN A P + ++ + IQ PP V S + VLE +V + HC+ PA + Y RW+
Sbjct: 56 DQVVVSGQPVTLLCAIPEYDGFVLWIKDGLALGVGRDLSSYPQYLVVGNHLSGEHHLKILRAELQDDAVYECQAIQAAIRS----RPARLTVLVPPDDPVI------LGGPVISLRAGD---PLNLTCHADNAKPAASIIWLRKGEVINGATYSKTLLRDGKRESI---VSTLFISPGDVENGQSIVCRATNK------AIPGGKETSVTIDIQHPPLVN--LSVEPQPVLEDNV-----VTFHCSAKANPAVTQY---RWA 285
BLAST of NPHS1-6 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 115.546 bits (288), Expect = 1.250e-25 Identity = 189/893 (21.16%), Postives = 362/893 (40.54%), Query Frame = 2
Query: 314 QWKANSD-LLGY--DRMIQG-RPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHP---ALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIESLSLSV---SKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHP-TGSVSSNGVQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENV----VQKINCITGEYYGSVNLKWKMDDLSRGKDLRSVEKIWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMFFMDQFWLKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIE--HNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFKRDSPSISTTDNKIFFQKVDESIKQ-------EYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKITELECLIESNPSPQISKINWLFFSSYNYFDENFDEIPKKYSCNQGHWKNKIMKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVAILLNTRSLAKAFMHS 2920
QWK+N+ LLGY + ++ G + R+ + ++ H L+I +V+++DDG+YECQ + HP +R + L IIV P++ +YF + N I+ + ++ P+ +TC+ + +PE WY++GK + VK L+ + + L+ + + I+C T + TL V+ PP+ P ++ N L N + + C T G P ++W + K + +D T++K I +++ F S+ + C A++ TG+ +++ + YPP + +F + N+ I C + + + W ++ S + + + S S L +I + C+ + K V +KI P + I E ++ + C + GG P +L W E + + N+ ++ S L+V R NT LKCE + SQ L+V +PP ++ I + LL + C T SNP + + W ++S + + L DV + T+ +G+ +++L + EY V+ C + +S + + L + + P + + ++ DS +++ V+ NP ++W +++ T ++F + S KQ Y L ATNS+G + + + + YS RI + LEC + PS + + I+W +N + + ++ +GH K I++L N + + G Y C A + + L+ S P + + + K A +L + A+ +++
Sbjct: 53 QWKSNTGSLLGYHQEGILPGHQGRFSYIKQNAEELH-LKITHVNLDDDGEYECQ-----MLHPEEGPIRAKSFLNIIVPPQL--VYFS-NYQPNSIIAVK---ENTPLNITCVVPNVKPEPEVLWYMDGKVMSRDVKQASTPHLNKTFTVYTSLVVQSDRNDHGKVITCEAFQKETDI--RITTNTTLDVLF--PPSDPTVEILRNPSALRSGDN--VTIACSVT-GGNPPPDVFW---YHENKRLQSHSTLD------TRSK--EIKNIYSFIASQNDNMAEYECRANNSRTGNPKRKAMKLEVNYPPASVE-----------------LFGESNIRYGSSANIQCKSLPSNPASQITWIINGRSVPTPTQREFVVENGIVSSSNVSVHSNELSVEAHQINVECMATNPEGSSAKQHV--IKIIAPPKAPIITGLEDRKFFEGDIVNVTCEAQGGNPLAELSWYRGSEKVNGAHNEVAGFSSYST-LALRVDRTMNTQRLKCEATNAALDEPLIESQ--YLSVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISW-----QFSRATDDNKVHLGDVS--LNETTRDNGFNVENVLSFTPTEEYDGTVVHCIANHPEWKHS---VNTSFPLNVMYPPKMLVNDPVTVVMSEGDSFKENLTVR--GNPAISLWQW--RKNGVPFDHTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAATGDTVLLECEADGEPS-KANMIHWY---------KNGEILAAQH---RGH-KKAILRL-------------------NATEQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKVYA 846
BLAST of NPHS1-6 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 110.923 bits (276), Expect = 3.233e-24 Identity = 191/905 (21.10%), Postives = 362/905 (40.00%), Query Frame = 2
Query: 314 QWKANSD-LLGY--DRMIQG-RPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHP---ALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIESLSLSV---SKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHP-TGSVSSNGVQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENV----VQKINCITGEYYGSVNLKWKMDDLSRGKDLRSVEKIWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMFFMDQFWLKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGET-IVKSENQGLFKTNNSVDFE-----------LKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIE--HNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFKRDSPSISTTDNKIFFQKVDESIKQ-------EYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKITELECLIESNPSPQISKINWLFFSSYNYFDENFDEIPKKYSCNQGHWKNKIMKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVAILLNTRSLAKAFMHS 2920
QWK+N+ LLGY + ++ G + R+ + ++ H L+I +V+++DDG+YECQ + HP +R + L IIV P++ +YF + N I+ + ++ P+ +TC+ + +PE WY++GK + VK L+ + + L+ + + I+C T + TL V+ PP+ P ++ N L N + + C T G P ++W + K + +D T++K I +++ F S+ + C A++ TG+ +++ + YPP + +F + N+ I C + + + W ++ S + + + S S L +I + C+ + K V +KI P + I E ++ + C + GG P +L W E +VK + + N E L+V R NT LKCE + SQ L+V +PP ++ I + LL + C T SNP + + W ++S + + L DV + T+ +G+ +++L + EY V+ C + +S + + L + + P + + ++ DS +++ V+ NP ++W +++ T ++F + S KQ Y L ATNS+G + + + + YS RI + LEC + PS + + I+W +N + + ++ +GH K I++L N + + G Y C A + + L+ S P + + + K A +L + A+ +++
Sbjct: 53 QWKSNTGSLLGYHQEGILPGHQGRFSYIKQNAEELH-LKITHVNLDDDGEYECQ-----MLHPEEGPIRAKSFLNIIVPPQL--VYFS-NYQPNSIIAVK---ENTPLNITCVVPNVKPEPEVLWYMDGKVMSRDVKQASTPHLNKTFTVYTSLVVQSDRNDHGKVITCEAFQKETDI--RITTNTTLDVLF--PPSDPTVEILRNPSALRSGDN--VTIACSVT-GGNPPPDVFW---YHENKRLQSHSTLD------TRSK--EIKNIYSFIASQNDNMAEYECRANNSRTGNPKRKAMKLEVNYPPASVE-----------------LFGESNIRYGSSANIQCKSLPSNPASQITWIINGRSVPTPTQREFVVENGIVSSSNVSVHSNELSVEAHQINVECMATNPEGSSAKQHV--IKIIAPPKAPIITGLEDRKFFEGDIVNVTCEAQGGNPLAELSWYRGSEKHLVKGRKKMKWSQVNGAHNEVAGFSSYSTLALRVDRTMNTQRLKCEATNAALDEPLIESQ--YLSVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISW-----QFSRATDDNKVHLGDVS--LNETTRDNGFNVENVLSFTPTEEYDGTVVHCIANHPEWKHS---VNTSFPLNVMYPPKMLVNDPVTVVMSEGDSFKENLTVR--GNPAISLWQW--RKNGVPFDHTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAATGDTVLLECEADGEPS-KANMIHWY---------KNGEILAAQH---RGH-KKAILRL-------------------NATEQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKVYA 859
BLAST of NPHS1-6 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 78.5666 bits (192), Expect = 2.167e-14 Identity = 97/447 (21.70%), Postives = 180/447 (40.27%), Query Frame = 2
Query: 1595 ENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIE--HNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFKRDSPSISTTDNKIFFQKVDESIKQ-------EYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKITELECLIESNPSPQISKINWLFFSSYNYFDENFDEIPKKYSCNQGHWKNKIMKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVAILLNTRSLAKA 2908
E ++ + C + GG P +L W E + + N+ ++ S L+V R NT LKCE + SQ L+V +PP ++ I + LL + C T SNP + + W+ +S + + L DV + T+ +G+ +++L + EY V+ C + +S + + L + + P + + ++ DS +++ V+ NP ++W +++ T ++F + S KQ Y L ATNS+G + + + + YS RI + LEC + PS + + I+W +N + + ++ +GH K I++L N + + G Y C A + + L+ S P + + + K A +L +AKA
Sbjct: 144 EGDIVNVTCEAQGGNPLAELSWYRGSEKVNGAHNEVAGFSSYST-LALRVDRTMNTQRLKCEATNAALDEPLIESQ--YLSVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQ-----FSRATDDNKVHLGDVS--LNETTRDNGFNVENVLSFTPTEEYDGTVVHCIANHPEWKHS---VNTSFPLNVMYPPKMLVNDPVTVVMSEGDSFKENLTVR--GNPAISLWQW--RKNGVPFDHTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAATGDTVLLECEADGEPS-KANMIHWY---------KNGEILAAQH---RGH-KKAILRL-------------------NATEQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILG--GVAKA 538
BLAST of NPHS1-6 vs. Ensembl Celegans
Match: syg-1 (Synaptogenesis protein syg-1 [Source:UniProtKB/Swiss-Prot;Acc:B1Q236]) HSP 1 Score: 77.7962 bits (190), Expect = 3.584e-14 Identity = 46/151 (30.46%), Postives = 74/151 (49.01%), Query Frame = 2
Query: 230 IVPFP-EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRM--IQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYV 673
IV P + + + +T ++C + +Q G VQW + LG DR + G RYR+VG + GE+ L+I NV++ DD + CQ+ + LT++V P PK+ + H I PI +C+SR G+P + W +
Sbjct: 25 IVEAPKDTLAAVGETAILTCRVEHQQGPVQWMKDDFGLGTDRDKPLPGNKRYRMVGSAANGEYNLEISNVTLFDDDDFACQISESDHAKAVVSSKAKLTVLVRPTPPKI-VKSHHSLKAIA-------GDPITQSCLSRKGKPPPTIGWAI 167
BLAST of NPHS1-6 vs. Ensembl Celegans
Match: syg-1 (Synaptogenesis protein syg-1 [Source:UniProtKB/Swiss-Prot;Acc:B1Q236]) HSP 1 Score: 77.411 bits (189), Expect = 4.120e-14 Identity = 46/151 (30.46%), Postives = 74/151 (49.01%), Query Frame = 2
Query: 230 IVPFP-EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRM--IQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYV 673
IV P + + + +T ++C + +Q G VQW + LG DR + G RYR+VG + GE+ L+I NV++ DD + CQ+ + LT++V P PK+ + H I PI +C+SR G+P + W +
Sbjct: 25 IVEAPKDTLAAVGETAILTCRVEHQQGPVQWMKDDFGLGTDRDKPLPGNKRYRMVGSAANGEYNLEISNVTLFDDDDFACQISESDHAKAVVSSKAKLTVLVRPTPPKI-VKSHHSLKAIA-------GDPITQSCLSRKGKPPPTIGWAI 167
BLAST of NPHS1-6 vs. Ensembl Fly
Match: sns (gene:FBgn0024189 transcript:FBtr0088635) HSP 1 Score: 149.058 bits (375), Expect = 1.176e-35 Identity = 191/805 (23.73%), Postives = 324/805 (40.25%), Query Frame = 2
Query: 218 QCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWY---VNGKPVCSAVKICEIESLSL-SVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHPTGSVSSNG----VQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRGKDLRSV--EKIWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMFFMDQFW-LKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKV--QRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPP--LKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMN--WEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFKRDSPSI-STTDNKIF-------FQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRI--VGERFMKLPIHSKITELECLIESNP 2551
Q F P V+E + + M C ++N G VQW + LG+ +I G PRY ++GD +G + L+I N SI DD Y+CQV P + A+R LT+I P + +G + N VE+ +++ ++L CI + +P WY V KP + E + + S L P +C + A P P T+++ + PP P I+ ++ + L ++++L C G P A L W S ++ Y T ++S +++ F ++ C A + +S N V++ + + P ++T+ P + + + C T +KW + G+ +R+ + I + + S+++ + P + + C M + ST TI + P I Y ++ V KL C+S GG P L W N + I N + + SV E+ + N +CE + T+ Q L+V F P +KI+IE P L + C ++ SNPP+ L W I N + K ++ G T S L+ +N E + V C+S S + L + ++P F+ + + + + +SL S+ +T ANP P+ +KW +D +I D++IF F ++ Y A+NS G + L V + Y I V E + P + L C +E P
Sbjct: 72 QKFRTTPHDLQVLEGAEAM-MRCEVANVAGAVQWTKDGFALGFSAVIPGFPRYSVLGDRKQGIYNLRISNASINDDADYQCQVGPARLNS-AIRANAKLTVISPPA--SIEIKG-YSHNSKVEVR---ENQDLQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVEESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKALS--PDMPMRATVQLSVLYPPGPPYIEGYSAGETLR--RGQTVELMCRSR-GGNPPAQLIWYKNGSQIRMAY-----------RTSGRLS--ENIYTFTAEAGDNKARFRCEASN---VMSQNPLKAEVELSVLFAP--THVTVMGPTEARVGD-----------IVPLTCTTAPSNPPAEIKWMVG----GRQVRNATSKTIVSPEGGWTTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDKRI----NSVIRAADKSVSAEITILANVSDNQAQYRCEA--SNSATEIPLFQSTTLSVHFAPETVKIRIE---PEELRPGMEATIICDSSSSNPPAKLSWWKDGIPIEGINNTS-------KPGLWGG-------TVSTLEFRVNVTQEMNGQVYTCQSANEALQRS---AHEAVSLDVLYRPKFVPPPSSTAVGVEGESLQVSL--QTRANPTPVTYKW--TKDGTTIPQDGDHRIFADGGSLNFTRLHRDDAGIYSCSASNSQGGATLNITVVVEYGTTIKSVSENIVVNPGEDAM--LSCTVEGKP 798
BLAST of NPHS1-6 vs. Ensembl Fly
Match: sns (gene:FBgn0024189 transcript:FBtr0339277) HSP 1 Score: 148.673 bits (374), Expect = 1.301e-35 Identity = 191/805 (23.73%), Postives = 325/805 (40.37%), Query Frame = 2
Query: 218 QCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWY---VNGKPVCSAVKICEIESLSL-SVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHPTGSVSSNG----VQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRGKDLRSV--EKIWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMFFMDQFW-LKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTS--QPIKLTVVFPP--LKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMN--WEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFKRDSPSI-STTDNKIF-------FQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRI--VGERFMKLPIHSKITELECLIESNP 2551
Q F P V+E + + M C ++N G VQW + LG+ +I G PRY ++GD +G + L+I N SI DD Y+CQV P + A+R LT+I P + +G + N VE+ +++ ++L CI + +P WY V KP + E + + S L P +C + A P P T+++ + PP P I+ ++ + L ++++L C G P A L W S ++ Y T ++S +++ F ++ C A + +S N V++ + + P ++T+ P + + + C T +KW + G+ +R+ + I + + S+++ + P + + C M + ST TI + P I Y ++ V KL C+S GG P L W N + I N + + SV E+ + + N + + R E + T Q L+V F P +KI+IE P L + C ++ SNPP+ L W I N + K ++ G T S L+ +N E + V C+S S + L + ++P F+ + + + + +SL S+ +T ANP P+ +KW +D +I D++IF F ++ Y A+NS G + L V + Y I V E + P + L C +E P
Sbjct: 72 QKFRTTPHDLQVLEGAEAM-MRCEVANVAGAVQWTKDGFALGFSAVIPGFPRYSVLGDRKQGIYNLRISNASINDDADYQCQVGPARLNS-AIRANAKLTVISPPA--SIEIKG-YSHNSKVEVR---ENQDLQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVEESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKALS--PDMPMRATVQLSVLYPPGPPYIEGYSAGETLR--RGQTVELMCRSR-GGNPPAQLIWYKNGSQIRMAY-----------RTSGRLS--ENIYTFTAEAGDNKARFRCEASN---VMSQNPLKAEVELSVLFAP--THVTVMGPTEARVGD-----------IVPLTCTTAPSNPPAEIKWMVG----GRQVRNATSKTIVSPEGGWTTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDKRI----NSVIRAADKSVSAEITI--LANVSDNQAQYRCEASNSATEIPLFQSTTLSVHFAPETVKIRIE---PEELRPGMEATIICDSSSSNPPAKLSWWKDGIPIEGINNTS-------KPGLWGG-------TVSTLEFRVNVTQEMNGQVYTCQSANEALQRS---AHEAVSLDVLYRPKFVPPPSSTAVGVEGESLQVSL--QTRANPTPVTYKW--TKDGTTIPQDGDHRIFADGGSLNFTRLHRDDAGIYSCSASNSQGGATLNITVVVEYGTTIKSVSENIVVNPGEDAM--LSCTVEGKP 798
BLAST of NPHS1-6 vs. Ensembl Fly
Match: sns (gene:FBgn0024189 transcript:FBtr0111043) HSP 1 Score: 148.673 bits (374), Expect = 1.529e-35 Identity = 191/805 (23.73%), Postives = 324/805 (40.25%), Query Frame = 2
Query: 218 QCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWY---VNGKPVCSAVKICEIESLSL-SVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHPTGSVSSNG----VQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRGKDLRSV--EKIWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMFFMDQFW-LKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKV--QRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPP--LKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMN--WEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFKRDSPSI-STTDNKIF-------FQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRI--VGERFMKLPIHSKITELECLIESNP 2551
Q F P V+E + + M C ++N G VQW + LG+ +I G PRY ++GD +G + L+I N SI DD Y+CQV P + A+R LT+I P + +G + N VE+ +++ ++L CI + +P WY V KP + E + + S L P +C + A P P T+++ + PP P I+ ++ + L ++++L C G P A L W S ++ Y T ++S +++ F ++ C A + +S N V++ + + P ++T+ P + + + C T +KW + G+ +R+ + I + + S+++ + P + + C M + ST TI + P I Y ++ V KL C+S GG P L W N + I N + + SV E+ + N +CE + T+ Q L+V F P +KI+IE P L + C ++ SNPP+ L W I N + K ++ G T S L+ +N E + V C+S S + L + ++P F+ + + + + +SL S+ +T ANP P+ +KW +D +I D++IF F ++ Y A+NS G + L V + Y I V E + P + L C +E P
Sbjct: 72 QKFRTTPHDLQVLEGAEAM-MRCEVANVAGAVQWTKDGFALGFSAVIPGFPRYSVLGDRKQGIYNLRISNASINDDADYQCQVGPARLNS-AIRANAKLTVISPPA--SIEIKG-YSHNSKVEVR---ENQDLQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVEESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKALS--PDMPMRATVQLSVLYPPGPPYIEGYSAGETLR--RGQTVELMCRSR-GGNPPAQLIWYKNGSQIRMAY-----------RTSGRLS--ENIYTFTAEAGDNKARFRCEASN---VMSQNPLKAEVELSVLFAP--THVTVMGPTEARVGD-----------IVPLTCTTAPSNPPAEIKWMVG----GRQVRNATSKTIVSPEGGWTTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDKRI----NSVIRAADKSVSAEITILANVSDNQAQYRCEA--SNSATEIPLFQSTTLSVHFAPETVKIRIE---PEELRPGMEATIICDSSSSNPPAKLSWWKDGIPIEGINNTS-------KPGLWGG-------TVSTLEFRVNVTQEMNGQVYTCQSANEALQRS---AHEAVSLDVLYRPKFVPPPSSTAVGVEGESLQVSL--QTRANPTPVTYKW--TKDGTTIPQDGDHRIFADGGSLNFTRLHRDDAGIYSCSASNSQGGATLNITVVVEYGTTIKSVSENIVVNPGEDAM--LSCTVEGKP 798
BLAST of NPHS1-6 vs. Ensembl Fly
Match: hbs (gene:FBgn0029082 transcript:FBtr0087413) HSP 1 Score: 133.65 bits (335), Expect = 4.509e-31 Identity = 178/822 (21.65%), Postives = 331/822 (40.27%), Query Frame = 2
Query: 215 IQCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSA--VKICEIESLSLSVSKLIFV-PNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTIS-SLHLFYISKKFHRGFLHCVADHPTGSVSSNG-VQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMD----DLSRGKDLRSVEKIWQIQSNYK----QQSSVSVTLGPTVD-EIRLNCVMFFMDQFWLKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETR-----LEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIW-----KIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFKRDSPSIST---------TDN-KIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKITELECLIESNPSPQISKINW 2578
+Q F + P ++E T+ + C +SN+ GKVQW + LG+ +I G PRY ++ D + + LQIKN ++EDD +Y+CQV P +PA+R L+I+ P + +G + N VE+ ++ + + L CI+ + P W+ PV +A V + + + S + + P + SC + A P P +++ + PP P + ++ + L + +++ C G P A L W +R +G+ + + +S +++ F + + + L C A + + + + + Y P+ + YL+ + K + ++V +++C+T + W ++ D S K S + W SN QS + + ++ E+ N V + T+ + P + Y +L +LKL+C S GG P P LQW N + I+ + ++ L + + + L V N I KC+ + + + TKT L V FPP +KI P L+ C ++ SNPP+ + W ++ +N +S ++ Y+ G V C+S S + I L I + P F S + ++ + + NP+ I + W +D IS+ +D ++ ++ + Y A NS G ++L V + Y+P I + + + L C I++ P + + + W
Sbjct: 38 VQKFHLTPHDLQILEGTDTL-LRCEVSNRAGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDDAEYQCQVGP-AAGNPAIRANAKLSIVAAPS--SIVIEG-YARNARVEV---EERQNLTLHCIAENANPAAEIVWFQGEVPVSTAPIVTVNQTAPKRFTTSSTLHLQPRAEDDYKEFSCEARHKALP--PDVPMRAQVQLSVLYPPGAPFFEGYSQGETLH--RGQEVQIACRSR-GGNPPAQLTW-YR-------------NGVAISSPQRTSGRLSENVYKFTAAAEDNGANLVCEAKNLLATTPLRAELNLTVLYAPKDV-------YLSGANQAK----VGDSV--QLSCVTAPSNPQARISWSINGRPLDNSTYKTTSSSDGGWVSSSNISLTIDSQSRTFIAVCHALNTELTQNVV----------GSHTVNVLYPPSPPLLTGYNDGDILISGSILKLQCSSAGGNPPPTLQWYKN-DKIINAPSK-LVDSKITSELSLLVNASDNNAIYKCKVQNAAIDIPLFATKT-------LGVHFPPETVKISV-VPKNLVPGIRAKLICDSSSSNPPAKISWWKDGIPVEGLNLANRPGLWGGSVSTLEMYVNITQDLDGI-----------------VYTCQSHNEVLQRS---VHETISLDILYPPKFETPQ--STTFVGVEGAPFHVELLASGNPMVITYTW--TKDGLPISSNSLSGQRLISDGPRLNISRLSRNDAGVYICEALNSQGTALLEIQVAVEYAPTITAVSEGRSFVAGEPAVLACHIQARPL-EAAHVRW 774
BLAST of NPHS1-6 vs. Ensembl Fly
Match: hbs (gene:FBgn0029082 transcript:FBtr0087414) HSP 1 Score: 133.265 bits (334), Expect = 6.938e-31 Identity = 178/822 (21.65%), Postives = 331/822 (40.27%), Query Frame = 2
Query: 215 IQCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSA--VKICEIESLSLSVSKLIFV-PNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTIS-SLHLFYISKKFHRGFLHCVADHPTGSVSSNG-VQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMD----DLSRGKDLRSVEKIWQIQSNYK----QQSSVSVTLGPTVD-EIRLNCVMFFMDQFWLKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETR-----LEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIW-----KIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFKRDSPSIST---------TDN-KIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKITELECLIESNPSPQISKINW 2578
+Q F + P ++E T+ + C +SN+ GKVQW + LG+ +I G PRY ++ D + + LQIKN ++EDD +Y+CQV P +PA+R L+I+ P + +G + N VE+ ++ + + L CI+ + P W+ PV +A V + + + S + + P + SC + A P P +++ + PP P + ++ + L + +++ C G P A L W +R +G+ + + +S +++ F + + + L C A + + + + + Y P+ + YL+ + K + ++V +++C+T + W ++ D S K S + W SN QS + + ++ E+ N V + T+ + P + Y +L +LKL+C S GG P P LQW N + I+ + ++ L + + + L V N I KC+ + + + TKT L V FPP +KI P L+ C ++ SNPP+ + W ++ +N +S ++ Y+ G V C+S S + I L I + P F S + ++ + + NP+ I + W +D IS+ +D ++ ++ + Y A NS G ++L V + Y+P I + + + L C I++ P + + + W
Sbjct: 38 VQKFHLTPHDLQILEGTDTL-LRCEVSNRAGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDDAEYQCQVGP-AAGNPAIRANAKLSIVAAPS--SIVIEG-YARNARVEV---EERQNLTLHCIAENANPAAEIVWFQGEVPVSTAPIVTVNQTAPKRFTTSSTLHLQPRAEDDYKEFSCEARHKALP--PDVPMRAQVQLSVLYPPGAPFFEGYSQGETLH--RGQEVQIACRSR-GGNPPAQLTW-YR-------------NGVAISSPQRTSGRLSENVYKFTAAAEDNGANLVCEAKNLLATTPLRAELNLTVLYAPKDV-------YLSGANQAK----VGDSV--QLSCVTAPSNPQARISWSINGRPLDNSTYKTTSSSDGGWVSSSNISLTIDSQSRTFIAVCHALNTELTQNVV----------GSHTVNVLYPPSPPLLTGYNDGDILISGSILKLQCSSAGGNPPPTLQWYKN-DKIINAPSK-LVDSKITSELSLLVNASDNNAIYKCKVQNAAIDIPLFATKT-------LGVHFPPETVKISV-VPKNLVPGIRAKLICDSSSSNPPAKISWWKDGIPVEGLNLANRPGLWGGSVSTLEMYVNITQDLDGI-----------------VYTCQSHNEVLQRS---VHETISLDILYPPKFETPQ--STTFVGVEGAPFHVELLASGNPMVITYTW--TKDGLPISSNSLSGQRLISDGPRLNISRLSRNDAGVYICEALNSQGTALLEIQVAVEYAPTITAVSEGRSFVAGEPAVLACHIQARPL-EAAHVRW 774
BLAST of NPHS1-6 vs. Ensembl Zebrafish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1]) HSP 1 Score: 135.961 bits (341), Expect = 1.138e-31 Identity = 187/897 (20.85%), Postives = 347/897 (38.68%), Query Frame = 2
Query: 251 VVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIESLSLSVSK---------LIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVA-DHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRGKDLRSVEKIWQIQSNYKQQSSVSVTLGPTVDE--IRLNCVMFFMD-QFWLKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLF------KRDSPSISTTDNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKI---TELECLIESNPSPQISKINWLFFSSYNYFDENFDEIPKKYSCNQGHWKNKIMKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVA 2875
V T + C + G VQW + LLG R + G PRY + GD KG++ LQI +V++EDDG YECQV P + R L + + P YFQ + + + + +TCI+ +P + +G+ ++ E ES ++S S+ + + + ++C N A+ R A T+KV PP P I NE E+ +K+ C + G P A+L+ W + E L+ T +D + S++ + + + + L C + + T S S + + + P + + L S + E + ++C T V+++W + K+L E + N + ++T + ++ + L C F +F ++ P++++++ L L+L C S GG P L W+ N + ++ + Q + S + L + N +C++ + K V S K V+FP + +KI L + + +C+ SNP + + W + ++ + +F G S L L++ + + C+ ++S+ + + L + F P F + + ++ ++ K ANP I +W+F K P + +D + V +Y + +N+ G + +D+ YSP + R P+ I +L C+ ++NP I F S+ + E + + S + G L IY + + G Y C+A + S + + +LI P+ P + KVA
Sbjct: 82 TVRAGATALLKCEVLRASGAVQWVKDGLLLGPLRSLPGYPRYSMTGDQKKGQYHLQILDVTLEDDGPYECQVGRSESSRPIVSRTVWLNVQIPSSQP--YFQLESEEPWV-------EGEQYTVTCIAPDAKPPAEITMFRDGE------ELLESESYTMSGSQDKLQNTHAEVTIRARSSDDTRRLTCRAKNPASPR--ALETGMTMKVYF--PPQAPMIMGLENE---EVKAGSFLKVVC-MSYGGNPLATLH--WTKNGEVLS-TSWDVD-----FVSRRASSVLKME---VKPEDNHAVLQCQSINQVTRSPMSLTRTLTVLFEPAEVTL------LGSFEAVEG-----EEI--NLSCSTSSSNPPVHIRWWLG----FKELNRTEVTFAEGDNGGMMTVSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELIVYYPPQKVWLDAPPPNVFLHSGSTLRLVCFSSGGNPTGQLTWLKNNKVVLTASKQVSSERGVSRELLLILHPSDNLATYRCDS---SNKAKKVLSTQTKFRVLFPAVSVKIVAK-QKELRRGQTLDLDCLAGSSNPTANISWSLGPVILKGVDQAPKR------------AEFGGMSRTSKLSLTLESHHWRKRITCQ---AFSNVLSEGVNNFYSLDVLFPPEFSD--EQPDEVQVIEDETATLPAKVSANPDEITCEWIFQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLDVQYSPTV---RMKSDPVFVNIGDTADLLCVADANP------ITSSMF-SWKWMGEGERDELGEESEDDG---------------------TGVLTIYEVTRDRAGVYQCTADNGISPTGSVEGQLIVRFAPDLQKGPQWRKVA 875
BLAST of NPHS1-6 vs. Ensembl Zebrafish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1]) HSP 1 Score: 135.576 bits (340), Expect = 1.510e-31 Identity = 187/897 (20.85%), Postives = 346/897 (38.57%), Query Frame = 2
Query: 251 VVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIESLSLSVSK---------LIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVA-DHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRGKDLRSVEKIWQIQSNYKQQSSVSVTLGPTVDE--IRLNCVMFFMD-QFWLKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLF------KRDSPSISTTDNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKI---TELECLIESNPSPQISKINWLFFSSYNYFDENFDEIPKKYSCNQGHWKNKIMKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVA 2875
V T + C + G VQW + LLG R + G PRY + GD KG++ LQI +V++EDDG YECQV P + R L + + P YFQ + + + + +TCI+ +P + +G+ ++ E ES ++S S+ + + + ++C N A+ R A T+KV PP P I NE E+ +K+ C G P A+L+ W + E L+ T +D + S++ + + + + L C + + T S S + + + P + + L S + E + ++C T V+++W + K+L E + N + ++T + ++ + L C F +F ++ P++++++ L L+L C S GG P L W+ N + ++ + Q + S + L + N +C++ + K V S K V+FP + +KI L + + +C+ SNP + + W + ++ + +F G S L L++ + + C+ ++S+ + + L + F P F + + ++ ++ K ANP I +W+F K P + +D + V +Y + +N+ G + +D+ YSP + R P+ I +L C+ ++NP I F S+ + E + + S + G L IY + + G Y C+A + S + + +LI P+ P + KVA
Sbjct: 37 TVRAGATALLKCEVLRASGAVQWVKDGLLLGPLRSLPGYPRYSMTGDQKKGQYHLQILDVTLEDDGPYECQVGRSESSRPIVSRTVWLNVQIPSSQP--YFQLESEEPWV-------EGEQYTVTCIAPDAKPPAEITMFRDGE------ELLESESYTMSGSQDKLQNTHAEVTIRARSSDDTRRLTCRAKNPASPR--ALETGMTMKVYF--PPQAPMIMGLENE---EVKAGSFLKVVCMSYG-GNPLATLH--WTKNGEVLS-TSWDVD-----FVSRRASSVLKME---VKPEDNHAVLQCQSINQVTRSPMSLTRTLTVLFEPAEVTL------LGSFEAVEG-----EEI--NLSCSTSSSNPPVHIRWWLG----FKELNRTEVTFAEGDNGGMMTVSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELIVYYPPQKVWLDAPPPNVFLHSGSTLRLVCFSSGGNPTGQLTWLKNNKVVLTASKQVSSERGVSRELLLILHPSDNLATYRCDS---SNKAKKVLSTQTKFRVLFPAVSVKIVAK-QKELRRGQTLDLDCLAGSSNPTANISWSLGPVILKGVDQAPKR------------AEFGGMSRTSKLSLTLESHHWRKRITCQ---AFSNVLSEGVNNFYSLDVLFPPEFSD--EQPDEVQVIEDETATLPAKVSANPDEITCEWIFQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLDVQYSPTV---RMKSDPVFVNIGDTADLLCVADANP------ITSSMF-SWKWMGEGERDELGEESEDDG---------------------TGVLTIYEVTRDRAGVYQCTADNGISPTGSVEGQLIVRFAPDLQKGPQWRKVA 830
BLAST of NPHS1-6 vs. Ensembl Zebrafish
Match: kirrel3l (kirre like nephrin family adhesion molecule 3, like [Source:ZFIN;Acc:ZDB-GENE-070831-1]) HSP 1 Score: 97.4413 bits (241), Expect = 5.748e-20 Identity = 80/277 (28.88%), Postives = 120/277 (43.32%), Query Frame = 2
Query: 245 EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIK-HPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAV---KICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVII--QTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFT 1057
+ VV Q++ + CVI G VQW + LG +R + G RY ++GDP GEH L I + + DD YECQ G++ H A LT++V P P + +V L P LTC + +P WY +G+ + +A+ + E +VS L VP + + ++ +C V N PA P + V I Q PP+V V +V E K+ C+ P + Y RWS + ++ D L T
Sbjct: 26 DQVVVAGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWVRYSLMGDPLSGEHSLVIDSAELGDDAIYECQATQAGLRSHRA-----KLTVLVPPSDPIVE------GGPVVRLKAH---TPHNLTCRASGAKPAAEITWYRDGEIMQNAIYSKSLMEDGKRETAVSMLPMVPEDSDSGRTYTCRVLN------PAAPAGRQTSVTINVQHPPSV-------TLSVQPQTVMEGAKVLFICSASANPEITGY---RWSKGGVPISEANGDSLEVT 272
BLAST of NPHS1-6 vs. Ensembl Zebrafish
Match: kirrel3l (kirre like nephrin family adhesion molecule 3, like [Source:ZFIN;Acc:ZDB-GENE-070831-1]) HSP 1 Score: 97.4413 bits (241), Expect = 5.880e-20 Identity = 80/277 (28.88%), Postives = 120/277 (43.32%), Query Frame = 2
Query: 245 EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIK-HPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAV---KICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVII--QTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFT 1057
+ VV Q++ + CVI G VQW + LG +R + G RY ++GDP GEH L I + + DD YECQ G++ H A LT++V P P + +V L P LTC + +P WY +G+ + +A+ + E +VS L VP + + ++ +C V N PA P + V I Q PP+V V +V E K+ C+ P + Y RWS + ++ D L T
Sbjct: 40 DQVVVAGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWVRYSLMGDPLSGEHSLVIDSAELGDDAIYECQATQAGLRSHRA-----KLTVLVPPSDPIVE------GGPVVRLKAH---TPHNLTCRASGAKPAAEITWYRDGEIMQNAIYSKSLMEDGKRETAVSMLPMVPEDSDSGRTYTCRVLN------PAAPAGRQTSVTINVQHPPSV-------TLSVQPQTVMEGAKVLFICSASANPEITGY---RWSKGGVPISEANGDSLEVT 286
BLAST of NPHS1-6 vs. Ensembl Zebrafish
Match: kirrel3l (kirre like nephrin family adhesion molecule 3, like [Source:ZFIN;Acc:ZDB-GENE-070831-1]) HSP 1 Score: 97.4413 bits (241), Expect = 6.116e-20 Identity = 80/277 (28.88%), Postives = 120/277 (43.32%), Query Frame = 2
Query: 245 EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIK-HPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAV---KICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVII--QTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFT 1057
+ VV Q++ + CVI G VQW + LG +R + G RY ++GDP GEH L I + + DD YECQ G++ H A LT++V P P + +V L P LTC + +P WY +G+ + +A+ + E +VS L VP + + ++ +C V N PA P + V I Q PP+V V +V E K+ C+ P + Y RWS + ++ D L T
Sbjct: 22 DQVVVAGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWVRYSLMGDPLSGEHSLVIDSAELGDDAIYECQATQAGLRSHRA-----KLTVLVPPSDPIVE------GGPVVRLKAH---TPHNLTCRASGAKPAAEITWYRDGEIMQNAIYSKSLMEDGKRETAVSMLPMVPEDSDSGRTYTCRVLN------PAAPAGRQTSVTINVQHPPSV-------TLSVQPQTVMEGAKVLFICSASANPEITGY---RWSKGGVPISEANGDSLEVT 268
BLAST of NPHS1-6 vs. Ensembl Xenopus
Match: NPHS1 (NPHS1 nephrin [Source:NCBI gene;Acc:100494620]) HSP 1 Score: 118.242 bits (295), Expect = 4.960e-26 Identity = 169/800 (21.12%), Postives = 316/800 (39.50%), Query Frame = 2
Query: 218 QCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKL--YFQGKHLS-----NQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHL-FYISKKFHRGFLHCVA-DHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRGKDLRSVEKIWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMFFMDQFWLKSTVTIMKIKR-PERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRI--HNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFK-----RDSPSIS--TTDNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSP--RIVGERFMKLPIHSKITELECLIESNPS 2554
Q F + P VV+ T +SC + + G VQW+ N LLG DR I G RY + GDP KGE+ L+I+ ++DD +Y CQV + + R + +++ P+ P Y G ++ V D +KP I + G V+G + ++E+ ++ V+K+I P + + ++C N A + P + + + PPN P I+ + V E++KL C + G P A+L W N+ + T+ + D ++ S HL I + + C A +H T + + + + P + I +S + N F +C T V ++W + + ++ + S++++ + L C F + KS + ++ P+ ++IE + +K+ C S GG P P L WI + +++ + V E+ + + N +C +T +T+ KL V FP + + I + Y ++ CVT SNP S + W +++ + D+ + + G + S + L + + + CE+ S + + + + +L I F P F++ + ++ ++ I V+ ANP I + W RD + + V + Y + N G + ++ +D+ YSP R +G+ + L E+ C ++NP+
Sbjct: 19 QVFRVQPDNITVVQ-GSTALLSCEVEHGTGMVQWEKNGLLLGPDRNIPGFLRYSMTGDPHKGEYNLRIERSELDDDAEYHCQVGRSEVSLGIISRTALINVLIPPKAPTFEEYAAGSRVTWVYGVEYTVTCQATD-AKPAAALTIKKSGL-------EVSGDSSSNPGSRDKLENTAM-VAKVI--PQSSDNGKLLTCLALNPALQT----PITVSFTMNVLFPPNAPIIEGYIGPSV---KAGETLKLIC-ISLSGNPLATLQWS---KNDDVVSTRWETD---------EVVRASRSHLTLNIKPADNMAVVSCEAVNHVTPETLKTSIILNVVFLPTEVTILGS----SSGPEKSNLTF---------SCFTAASNPPVQIRWWLGPKELVNTIVTISD--AVHGGKVTMSNLTMEALREEHGMALTCEAFNEAILYTKSNSVTLSVQYPPQNVWIEAPPADKFFRAGTSVKMTCFSSGGNPPPRLTWIKDTKSLSGGSQ---IHSGKIVTKEITIITVPSDNMATYRCNAS-NIGKTPALTAS-TKLKVQFPAIDVNIISSAKEY-RRGSTIILTCVTGSSNPASTISW---------VKDGEQLKAQDLGR---KPSLYGGISSSSRVTLKAASKDNGRRVSCEAFSSVLNEA---VNTFYKLNILFPPEFLD--DQPAVVQAVEHGAALIPVRVRANPPQINYTWSLWGEKLIRDGAYRHHLRGEGALEIWNVSRADSGVYNVTCVNKEGKNSIVIRLDVQYSPTIRSLGDTEVDL---GSDAEIACTADANPA 745
BLAST of NPHS1-6 vs. Ensembl Xenopus
Match: eif4e (eukaryotic translation initiation factor 4E [Source:Xenbase;Acc:XB-GENE-855435]) HSP 1 Score: 99.3673 bits (246), Expect = 1.076e-20 Identity = 64/212 (30.19%), Postives = 102/212 (48.11%), Query Frame = 2
Query: 251 VVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIES---LSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNV 877
V+ +++ + CV+ G VQW + LG +R + G RY +VGDPS GEH L I+ V + DD YECQ ++ R LT+++ P P + G ++N ++ + P LTC++ +P WY +GK + SAV E+ S S S L+ P+ + S +C V N A +++ + +Q PP V
Sbjct: 38 VIVAGKSVTLPCVVIGYRGVVQWTMDGLALGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQATQAALRSQRAR----LTVLLPPGDPMI--PGSPVANVLLNV-------PYNLTCLAPVAKPAAEITWYRDGKKLDSAVYTKELLSDGKSEGSASSLLITPSLADSGSSYTCRVRNEALPE----GREKSVTLSVQYPPKV 232
BLAST of NPHS1-6 vs. Ensembl Xenopus
Match: eif4e (eukaryotic translation initiation factor 4E [Source:Xenbase;Acc:XB-GENE-855435]) HSP 1 Score: 98.9821 bits (245), Expect = 1.676e-20 Identity = 64/212 (30.19%), Postives = 102/212 (48.11%), Query Frame = 2
Query: 251 VVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIES---LSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNV 877
V+ +++ + CV+ G VQW + LG +R + G RY +VGDPS GEH L I+ V + DD YECQ ++ R LT+++ P P + G ++N ++ + P LTC++ +P WY +GK + SAV E+ S S S L+ P+ + S +C V N A +++ + +Q PP V
Sbjct: 38 VIVAGKSVTLPCVVIGYRGVVQWTMDGLALGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQATQAALRSQRAR----LTVLLPPGDPMI--PGSPVANVLLNV-------PYNLTCLAPVAKPAAEITWYRDGKKLDSAVYTKELLSDGKSEGSASSLLITPSLADSGSSYTCRVRNEALPE----GREKSVTLSVQYPPKV 232
BLAST of NPHS1-6 vs. Ensembl Xenopus
Match: eif4e (eukaryotic translation initiation factor 4E [Source:Xenbase;Acc:XB-GENE-855435]) HSP 1 Score: 98.9821 bits (245), Expect = 1.971e-20 Identity = 64/212 (30.19%), Postives = 102/212 (48.11%), Query Frame = 2
Query: 251 VVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIES---LSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNV 877
V+ +++ + CV+ G VQW + LG +R + G RY +VGDPS GEH L I+ V + DD YECQ ++ R LT+++ P P + G ++N ++ + P LTC++ +P WY +GK + SAV E+ S S S L+ P+ + S +C V N A +++ + +Q PP V
Sbjct: 46 VIVAGKSVTLPCVVIGYRGVVQWTMDGLALGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQATQAALRSQRAR----LTVLLPPGDPMI--PGSPVANVLLNV-------PYNLTCLAPVAKPAAEITWYRDGKKLDSAVYTKELLSDGKSEGSASSLLITPSLADSGSSYTCRVRNEALPEG----REKSVTLSVQYPPKV 240
BLAST of NPHS1-6 vs. Ensembl Xenopus
Match: eif4e (eukaryotic translation initiation factor 4E [Source:Xenbase;Acc:XB-GENE-855435]) HSP 1 Score: 98.5969 bits (244), Expect = 1.984e-20 Identity = 64/212 (30.19%), Postives = 102/212 (48.11%), Query Frame = 2
Query: 251 VVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIES---LSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNV 877
V+ +++ + CV+ G VQW + LG +R + G RY +VGDPS GEH L I+ V + DD YECQ ++ R LT+++ P P + G ++N ++ + P LTC++ +P WY +GK + SAV E+ S S S L+ P+ + S +C V N A +++ + +Q PP V
Sbjct: 34 VIVAGKSVTLPCVVIGYRGVVQWTMDGLALGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQATQAALRSQRAR----LTVLLPPGDPMI--PGSPVANVLLNV-------PYNLTCLAPVAKPAAEITWYRDGKKLDSAVYTKELLSDGKSEGSASSLLITPSLADSGSSYTCRVRNEALPEG----REKSVTLSVQYPPKV 228
BLAST of NPHS1-6 vs. Ensembl Mouse
Match: Nphs1 (nephrosis 1, nephrin [Source:MGI Symbol;Acc:MGI:1859637]) HSP 1 Score: 120.553 bits (301), Expect = 7.440e-27 Identity = 175/798 (21.93%), Postives = 320/798 (40.10%), Query Frame = 2
Query: 251 VVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKP---VCSAVKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIK-SFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVA-DHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRGKDLRSVEK--IWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMF---FMDQFWLKSTVTIMKIKRP-ERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETI---------VKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVT-SQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFK--RDSPSIST-----TDNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKI---TELECLIESNP 2551
VVE T+++ C + VQW + LLG + I G PRY + GD +KGE L I+ + DD +YECQV + + L+I+V P++ +L + + + +TC+S +P + G+ V S+V E L + ++ P + Q + C +N A A P + + I PP P I NE + E+++L C G P A+L W N + I T++ + S+ + + + H L C + + + + + + +PP + I L S +++N +NV + C+T V L+W + G+ L ++ + + + S++++ + + + L C F F + + KS + +K P ++L+IE + ++L CL+ GG P+P L W+ + + + + + K+ ++ EL +I+ L + K S +L V FPP + I N + L + + CV+ SNPP L +++ E L DV + F G + L ++ H + C ++S+ + S +L + + P F +G + + ++ + V ANP P F W F+ R SP+ + + V + YQL NS G + + +D+ Y+P I R +K P + ++ C +++NP
Sbjct: 57 VVE-GSTVKLWCGVRAPGSVVQWAKDGLLLGPNPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPSVILSILVSPKVLQLTPEAGSTVTWVA-------GQEYVVTCVSGDAKPAPDIIFIQGGRTVEDVSSSVNEGSEEKLFFTEAEARVTPQSSDNGQLLVCEGSNPAL----ATPIKASFTMNILFPPGPPVIDWPGLNEG--HVRAGENLELPCIAR-GGNPPATLQW-------LKNGKPVSI----AWGTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAETQERSITLQVTFPPSAVTI------LGSTSQSEN-----KNVT--LCCLTKSSRPRVLLRWWLG----GRQLLPTDETVMDGLHGGHISMSNLTLLVKREDNGLSLTCEAFSDAFSKETFKKSLT--LNVKYPAQKLWIEGPPEGQSIRTGTRVRLVCLAIGGNPEPSLTWLKDSRPVNDPRQSQEPRRVQLGSVEKSGSTFSREL-------VLIIGPPDNLAKFSCKAGQLSASTQLVVQFPPTNLTILANS-SALRPGDALNLTCVSISSNPPVNL----------SLDKEGER-LDDVAAKPQS-APFKGSAASRSVFLRVSSRDHGHRVTCR---AHSEALRETVSSFYRLNVLYPPEF--LGEQVRAVTVVEQGQALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTI---RALKDPTEVNVGGSVDIVCTVDANP 781
BLAST of NPHS1-6 vs. Ensembl Mouse
Match: Nphs1 (nephrosis 1, nephrin [Source:MGI Symbol;Acc:MGI:1859637]) HSP 1 Score: 120.553 bits (301), Expect = 7.506e-27 Identity = 175/798 (21.93%), Postives = 320/798 (40.10%), Query Frame = 2
Query: 251 VVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKP---VCSAVKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIK-SFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVA-DHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRGKDLRSVEK--IWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMF---FMDQFWLKSTVTIMKIKRP-ERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETI---------VKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVT-SQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFK--RDSPSIST-----TDNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKI---TELECLIESNP 2551
VVE T+++ C + VQW + LLG + I G PRY + GD +KGE L I+ + DD +YECQV + + L+I+V P++ +L + + + +TC+S +P + G+ V S+V E L + ++ P + Q + C +N A A P + + I PP P I NE + E+++L C G P A+L W N + I T++ + S+ + + + H L C + + + + + + +PP + I L S +++N +NV + C+T V L+W + G+ L ++ + + + S++++ + + + L C F F + + KS + +K P ++L+IE + ++L CL+ GG P+P L W+ + + + + + K+ ++ EL +I+ L + K S +L V FPP + I N + L + + CV+ SNPP L +++ E L DV + F G + L ++ H + C ++S+ + S +L + + P F +G + + ++ + V ANP P F W F+ R SP+ + + V + YQL NS G + + +D+ Y+P I R +K P + ++ C +++NP
Sbjct: 43 VVE-GSTVKLWCGVRAPGSVVQWAKDGLLLGPNPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPSVILSILVSPKVLQLTPEAGSTVTWVA-------GQEYVVTCVSGDAKPAPDIIFIQGGRTVEDVSSSVNEGSEEKLFFTEAEARVTPQSSDNGQLLVCEGSNPAL----ATPIKASFTMNILFPPGPPVIDWPGLNEG--HVRAGENLELPCIAR-GGNPPATLQW-------LKNGKPVSI----AWGTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAETQERSITLQVTFPPSAVTI------LGSTSQSEN-----KNVT--LCCLTKSSRPRVLLRWWLG----GRQLLPTDETVMDGLHGGHISMSNLTLLVKREDNGLSLTCEAFSDAFSKETFKKSLT--LNVKYPAQKLWIEGPPEGQSIRTGTRVRLVCLAIGGNPEPSLTWLKDSRPVNDPRQSQEPRRVQLGSVEKSGSTFSREL-------VLIIGPPDNLAKFSCKAGQLSASTQLVVQFPPTNLTILANS-SALRPGDALNLTCVSISSNPPVNL----------SLDKEGER-LDDVAAKPQS-APFKGSAASRSVFLRVSSRDHGHRVTCR---AHSEALRETVSSFYRLNVLYPPEF--LGEQVRAVTVVEQGQALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTI---RALKDPTEVNVGGSVDIVCTVDANP 767
BLAST of NPHS1-6 vs. Ensembl Mouse
Match: Kirrel3 (kirre like nephrin family adhesion molecule 3 [Source:MGI Symbol;Acc:MGI:1914953]) HSP 1 Score: 88.5817 bits (218), Expect = 3.058e-17 Identity = 77/262 (29.39%), Postives = 118/262 (45.04%), Query Frame = 2
Query: 245 EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSA------VKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQ--TLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWS 1006
+ VV Q + + C I G V W + LG R + P+Y +VG+ GEH L+I ++DD YECQ I+ R LT++V P+ P + L ++ L D P+ LTC + + +P S W G+ + A ++ + ES+ VS L P +++ QSI C TN A P + ++ + IQ PP V S + VLE ++I F HC+ PA + Y RW+
Sbjct: 23 DQVVVSGQPVTLLCAIPEYDGFVLWIKDGLALGVGRDLSSYPQYLVVGNHLSGEHHLKILRAELQDDAVYECQAIQAAIRS----RPARLTVLVPPDDPII------LGGPVISLRAGD---PLNLTCHADNAKPAASIIWLRKGEVINGATYSKTLLRDGKRESI---VSTLFISPGDVENGQSIVCRATNK------AIPGGKETSVTIDIQHPPLVN--LSVEPQPVLE----DNIVTF-HCSAKANPAVTQY---RWA 252
BLAST of NPHS1-6 vs. Ensembl Mouse
Match: Kirrel3 (kirre like nephrin family adhesion molecule 3 [Source:MGI Symbol;Acc:MGI:1914953]) HSP 1 Score: 88.5817 bits (218), Expect = 3.058e-17 Identity = 77/262 (29.39%), Postives = 118/262 (45.04%), Query Frame = 2
Query: 245 EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSA------VKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQ--TLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWS 1006
+ VV Q + + C I G V W + LG R + P+Y +VG+ GEH L+I ++DD YECQ I+ R LT++V P+ P + L ++ L D P+ LTC + + +P S W G+ + A ++ + ES+ VS L P +++ QSI C TN A P + ++ + IQ PP V S + VLE ++I F HC+ PA + Y RW+
Sbjct: 23 DQVVVSGQPVTLLCAIPEYDGFVLWIKDGLALGVGRDLSSYPQYLVVGNHLSGEHHLKILRAELQDDAVYECQAIQAAIRS----RPARLTVLVPPDDPII------LGGPVISLRAGD---PLNLTCHADNAKPAASIIWLRKGEVINGATYSKTLLRDGKRESI---VSTLFISPGDVENGQSIVCRATNK------AIPGGKETSVTIDIQHPPLVN--LSVEPQPVLE----DNIVTF-HCSAKANPAVTQY---RWA 252
BLAST of NPHS1-6 vs. Ensembl Mouse
Match: Kirrel3 (kirre like nephrin family adhesion molecule 3 [Source:MGI Symbol;Acc:MGI:1914953]) HSP 1 Score: 88.1965 bits (217), Expect = 3.920e-17 Identity = 77/262 (29.39%), Postives = 118/262 (45.04%), Query Frame = 2
Query: 245 EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSA------VKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQ--TLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWS 1006
+ VV Q + + C I G V W + LG R + P+Y +VG+ GEH L+I ++DD YECQ I+ R LT++V P+ P + L ++ L D P+ LTC + + +P S W G+ + A ++ + ES+ VS L P +++ QSI C TN A P + ++ + IQ PP V S + VLE ++I F HC+ PA + Y RW+
Sbjct: 23 DQVVVSGQPVTLLCAIPEYDGFVLWIKDGLALGVGRDLSSYPQYLVVGNHLSGEHHLKILRAELQDDAVYECQAIQAAIRS----RPARLTVLVPPDDPII------LGGPVISLRAGD---PLNLTCHADNAKPAASIIWLRKGEVINGATYSKTLLRDGKRESI---VSTLFISPGDVENGQSIVCRATNK------AIPGGKETSVTIDIQHPPLVN--LSVEPQPVLE----DNIVTF-HCSAKANPAVTQY---RWA 252
BLAST of NPHS1-6 vs. UniProt/SwissProt
Match: sp|Q9QZS7|NPHN_MOUSE (Nephrin OS=Mus musculus OX=10090 GN=Nphs1 PE=1 SV=2) HSP 1 Score: 120.553 bits (301), Expect = 5.204e-26 Identity = 175/798 (21.93%), Postives = 320/798 (40.10%), Query Frame = 2
Query: 251 VVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKP---VCSAVKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIK-SFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVA-DHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRGKDLRSVEK--IWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMF---FMDQFWLKSTVTIMKIKRP-ERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETI---------VKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVT-SQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFK--RDSPSIST-----TDNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKI---TELECLIESNP 2551
VVE T+++ C + VQW + LLG + I G PRY + GD +KGE L I+ + DD +YECQV + + L+I+V P++ +L + + + +TC+S +P + G+ V S+V E L + ++ P + Q + C +N A A P + + I PP P I NE + E+++L C G P A+L W N + I T++ + S+ + + + H L C + + + + + + +PP + I L S +++N +NV + C+T V L+W + G+ L ++ + + + S++++ + + + L C F F + + KS + +K P ++L+IE + ++L CL+ GG P+P L W+ + + + + + K+ ++ EL +I+ L + K S +L V FPP + I N + L + + CV+ SNPP L +++ E L DV + F G + L ++ H + C ++S+ + S +L + + P F +G + + ++ + V ANP P F W F+ R SP+ + + V + YQL NS G + + +D+ Y+P I R +K P + ++ C +++NP
Sbjct: 57 VVE-GSTVKLWCGVRAPGSVVQWAKDGLLLGPNPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPSVILSILVSPKVLQLTPEAGSTVTWVA-------GQEYVVTCVSGDAKPAPDIIFIQGGRTVEDVSSSVNEGSEEKLFFTEAEARVTPQSSDNGQLLVCEGSNPAL----ATPIKASFTMNILFPPGPPVIDWPGLNEG--HVRAGENLELPCIAR-GGNPPATLQW-------LKNGKPVSI----AWGTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAETQERSITLQVTFPPSAVTI------LGSTSQSEN-----KNVT--LCCLTKSSRPRVLLRWWLG----GRQLLPTDETVMDGLHGGHISMSNLTLLVKREDNGLSLTCEAFSDAFSKETFKKSLT--LNVKYPAQKLWIEGPPEGQSIRTGTRVRLVCLAIGGNPEPSLTWLKDSRPVNDPRQSQEPRRVQLGSVEKSGSTFSREL-------VLIIGPPDNLAKFSCKAGQLSASTQLVVQFPPTNLTILANS-SALRPGDALNLTCVSISSNPPVNL----------SLDKEGER-LDDVAAKPQS-APFKGSAASRSVFLRVSSRDHGHRVTCR---AHSEALRETVSSFYRLNVLYPPEF--LGEQVRAVTVVEQGQALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTI---RALKDPTEVNVGGSVDIVCTVDANP 781
BLAST of NPHS1-6 vs. UniProt/SwissProt
Match: sp|Q9R044|NPHN_RAT (Nephrin OS=Rattus norvegicus OX=10116 GN=Nphs1 PE=1 SV=2) HSP 1 Score: 119.398 bits (298), Expect = 1.088e-25 Identity = 177/835 (21.20%), Postives = 333/835 (39.88%), Query Frame = 2
Query: 269 TIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGK---PVCSAVKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIK-SFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVA-DHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRGKDLRSVEK--IWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMF---FMDQFWLKSTVTIMKIKRP-ERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSEN---------QGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFK--RDSPSIST-----TDNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKI---TELECLIESNP-SPQISKINWLFFSSYNYFDENFDEIPKKYSCNQGHWKNKIMKL 2680
T+++ C + VQW + LLG + + G PRY + GD +KGE L I+ + DD +YECQV + + L+I+V P++ L + + + +TC+S +P + +G+ V S V E L ++ ++ +P + Q + C +N A P + + I PP P I NE + E+++L C G P A+L W N K T T++ + S+ + + + H L C + + + + + + +PP I I L S+ +++N +NV + C+T V L+W + G+ L ++ + + + S+++ + + + L C F F + + KS + +K P ++L+IE + ++L CL+ GG P P L W + + + + K+ ++ EL + +I + R + S +L V FPP + I N + L + + CV+ SNPP L W + + E + + + F G+ ++S+ L ++ H + C ++S+ + S + + + P F +G + + ++ + V ANP P F W F+ R SP+ + + V + YQL NS G + + +D+ Y+P I R ++ P + ++ C +++NP P++ +W D N D++ K + G + + KL
Sbjct: 62 TVKLWCGVRAPGSVVQWAKDGLLLGPNPKMPGFPRYSLEGDRAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPKVILSILVSPKVLLLTPEAGSTVTWVA-------GQEYVVTCVSGDAKPAPDITFIQSGRTILDVSSNVNEGSEEKLCITEAEARVIPQSSDNGQLLVCEGSNPALDT----PIKASFTMNILFPPGPPVIDWPGLNEG--HVRAGENLELPCTAR-GGNPPATLQW---LKNGKPVSTAWG--------TEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAGTQERSITLQVTFPPSAITI------LGSVSQSEN-----KNVT--LCCLTKSSRPRVLLRWWLG----GRQLLPTDETVMDGLHGGHISMSNLTFLVRREDNGLPLTCEAFSDAFSKETFKKSLT--LNVKYPAQKLWIEGPPEGQYIRTGTRVRLVCLAIGGNPDPSLIWFKDSRPVSEPRQPQEPRRVQLGSVEKSGSTFSRELVL------IIGPPDNRAKFSCKAGQLSASTQLVVQFPPTNLTILANS-SALRPGDALNLTCVSISSNPPVNLSWDKEGERL-------EDVAAKPQSAPFKGSA----ASRSVF-LRVSSRDHGQRVTCR---AHSEALRETVSSFYRFNVLYPPEF--LGEQVRAVTVVEQGQVLLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTI---RALRDPTEVNVGGSVDIVCTVDANPILPEM--FSWERLGEEEE-DLNLDDMEKVSKGSTGRLRIRQAKL 822
BLAST of NPHS1-6 vs. UniProt/SwissProt
Match: sp|O60500|NPHN_HUMAN (Nephrin OS=Homo sapiens OX=9606 GN=NPHS1 PE=1 SV=1) HSP 1 Score: 109.768 bits (273), Expect = 1.061e-22 Identity = 150/683 (21.96%), Postives = 276/683 (40.41%), Query Frame = 2
Query: 218 QCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKP---VCSAVKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHP-TGSVSSNGVQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRGKDLRSVEK--IWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMF---FMDQFWLKSTVTIMKIKRP-ERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNT-------VILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFIN 2215
+ F +P VVE ++E+ C +S VQW + LLG D I G PRYR+ GDP++GE L I+ + DD +YECQV G ++ I+ + PKL L+ + + T + + C+S +P ++G+ + + V + L + P + Q + C ++ A P + V + PP P I+ + + +S++L C G P A+L W N + T T++ + S+ + + + H L C A + + +G+ + + +PP I I L S + +N +NV ++C++ V L+W + + L +E+ + + + S+++ + + L C F F + + KS I+ +K P ++L+IE L ++L CL+ GG P+P L W + T+ +S L + + V V++ +T V + + + S +L V FPP + I N + L + + CV+ SNPP L W + + E + + + F G+ +S+L L ++ H + C ++S + S +L + ++P F+
Sbjct: 32 RGFWALPENLTVVE-GASVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQV---GRSEMGPELVSPRVILSILVPPKLLL----LTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPALE----APIKASFTVNVLFPPGPPVIE-WPGLDEGHVRAGQSLELPC-VARGGNPLATLQW---LKNGQPVSTAWG--------TEHTQAVARSVLVMTVRPEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIII------LGSASQTEN-----KNVT--LSCVSKSSRPRVLLRWWLG----WRQLLPMEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAFSEAFTKETFKKS--LILNVKYPAQKLWIEGPPEGQKLRAGTRVRLVCLAIGGNPEPSLMWYKDSRTVTESR---LPQESRRVHLG-SVEKSGSTFSRELVLVTGPSDNQAKFTCKAGQLSASTQLAVQFPPTNVTILANA-SALRPGDALNLTCVSVSSNPPVNLSWDKEGERL-------EGVAAPPRRAPFKGSA----AARSVL-LQVSSRDHGQRVTCR---AHSAELRETVSSFYRLNVLYRPEFLG 650
BLAST of NPHS1-6 vs. UniProt/SwissProt
Match: sp|Q8IZU9|KIRR3_HUMAN (Kin of IRRE-like protein 3 OS=Homo sapiens OX=9606 GN=KIRREL3 PE=1 SV=1) HSP 1 Score: 88.5817 bits (218), Expect = 2.516e-16 Identity = 76/260 (29.23%), Postives = 116/260 (44.62%), Query Frame = 2
Query: 245 EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSA------VKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWS 1006
+ VV Q + + C I G V W + LG R + P+Y +VG+ GEH L+I ++DD YECQ I+ R LT++V P+ P + L ++ L D P+ LTC + + +P S W G+ + A ++ + ES+ VS L P +++ QSI C TN A P ++ + IQ PP V S + VLE +V + HC+ PA + Y RW+
Sbjct: 56 DQVVVSGQPVTLLCAIPEYDGFVLWIKDGLALGVGRDLSSYPQYLVVGNHLSGEHHLKILRAELQDDAVYECQAIQAAIRS----RPARLTVLVPPDDPVI------LGGPVISLRAGD---PLNLTCHADNAKPAASIIWLRKGEVINGATYSKTLLRDGKRESI---VSTLFISPGDVENGQSIVCRATNKA---IPG-GKETSVTIDIQHPPLVN--LSVEPQPVLEDNV-----VTFHCSAKANPAVTQY---RWA 285
BLAST of NPHS1-6 vs. UniProt/SwissProt
Match: sp|Q8BR86|KIRR3_MOUSE (Kin of IRRE-like protein 3 OS=Mus musculus OX=10090 GN=Kirrel3 PE=1 SV=1) HSP 1 Score: 88.1965 bits (217), Expect = 2.836e-16 Identity = 77/262 (29.39%), Postives = 118/262 (45.04%), Query Frame = 2
Query: 245 EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSA------VKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQ--TLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWS 1006
+ VV Q + + C I G V W + LG R + P+Y +VG+ GEH L+I ++DD YECQ I+ R LT++V P+ P + L ++ L D P+ LTC + + +P S W G+ + A ++ + ES+ VS L P +++ QSI C TN A P + ++ + IQ PP V S + VLE ++I F HC+ PA + Y RW+
Sbjct: 56 DQVVVSGQPVTLLCAIPEYDGFVLWIKDGLALGVGRDLSSYPQYLVVGNHLSGEHHLKILRAELQDDAVYECQAIQAAIRS----RPARLTVLVPPDDPII------LGGPVISLRAGD---PLNLTCHADNAKPAASIIWLRKGEVINGATYSKTLLRDGKRESI---VSTLFISPGDVENGQSIVCRATNK------AIPGGKETSVTIDIQHPPLVN--LSVEPQPVLE----DNIVTF-HCSAKANPAVTQY---RWA 285
BLAST of NPHS1-6 vs. TrEMBL
Match: A0A0H3YFQ6 (NPHS1-6 OS=Schmidtea mediterranea OX=79327 GN=NPHS1-6 PE=2 SV=1) HSP 1 Score: 422.935 bits (1086), Expect = 1.762e-123 Identity = 300/1028 (29.18%), Postives = 490/1028 (47.67%), Query Frame = 2
Query: 143 DLENSQEARIKQSDNCTQDDIKDNIQCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLK----VIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWR-------WSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNS--IYK---NKNYIFLDENVVQKINCITGEYYGSVNLKWKMD-DLSRGKDLRS-VEKIWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMFFMDQFW-----LKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVV-FNCVTTESNPPSGLIWKIKVCKYSNIE--------NCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKW------LFKRDSPSISTTDNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKITELECLIESNPSPQISKINWLFF---SSYN--------------------YFDENFDEIPKKYSCNQGHWKNKI----MKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVAILLNTRSLAKAFMHSGLTSNITLHIELH---LNKSVLFPVKVNRINSGIYLV 3022
D E S A+IKQS CT ++I + IQCF +P P Y V + C++ NQ+GKVQW+A LLGYDR I GRP YR+VG KGEH L+I NV+++D G YECQVQP+G +HP LRR T L ++V P+ P+++++G + V +++ Q E TC+S +G+P+V F+W++N + +E L+ S S+L + K I C V+N AT + P ++L ++I TPP P+I K V+++I + C +P G PA SL W W+ ++N NYT I Y+ T +K +S+L + ++ K H L CVA +P S S V++ I + P + I++ + L+S I K N+N I+ + + I C T YY +KW + + D S VEKIW+ ++++ S++ + + D+ L+C Q W ++S V + PE+ I+ Y ++ N ++ C + GG P P+LQW + + I S ++ + N V C+ + ++ + S + L V+FPP+ I ++ + +EN ++ C ESNPP+ + WK+ C E C E +LS G +F G +S ++ +W+ HL + CE + V+R +IQ+ ++F P F I N++ + +Q++ ++ +ANP + W L +++ SIST+ K+ + Y L+ N G+S + ++ITY P I+G++F + I + ELEC ++SNP+ W YN + E +E PK YS + WK K F KC + S + S LIIY+ DVG Y C ++ KTIEL P +S+P F+K A L ++ S + +T + ++ + + + + R +G Y+
Sbjct: 39 DSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQD-PERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDC------QAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLK-SLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTING-KFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYN--FYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYS--KAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVA 1053 HSP 2 Score: 52.7582 bits (125), Expect = 1.762e-123 Identity = 45/136 (33.09%), Postives = 69/136 (50.74%), Query Frame = 1
Query: 3001 QFRNIFSDFRIQLSDFGR--HRKNLFRAENKIGSSSLMLEAVSESIPDPPIVHEIKFLQPNSLFVKIIQTFDGGYPQSFQVFWKEANRNTTENKMWEVPGDYTK---KFIIIKI-SDTFLSKNYEIQVCSKNLVYG 3390
+FR F ++++ + R H + +AEN GS S +E S PD P+ ++ + + + IQ FDGG Q+FQV WKE + T+ + +V D TK FII + SDT Y I + S+N +G
Sbjct: 1045 RFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSP-TSLYRYEDVASDDTKITLSFIITGLKSDTA----YLISINSRNSKHG 1175
BLAST of NPHS1-6 vs. TrEMBL
Match: A0A4E0RDV1 (Nephrin OS=Fasciola hepatica OX=6192 GN=D915_004791 PE=4 SV=1) HSP 1 Score: 350.517 bits (898), Expect = 1.293e-95 Identity = 261/1032 (25.29%), Postives = 442/1032 (42.83%), Query Frame = 2
Query: 164 ARIKQSDNCTQDDIKDNIQCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGI-KHPALRRITHLTIIVLPEIPK-LYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIESLSL------------------SVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLK----VIIQTPPNVPEIKSFTNEKV-LEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLN--------------------YTKIK-------------------------------IDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHPTGSVSSNGVQIMIKYPPEFINITIDNPYLN---SIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRG-----KDLRSVEKIWQIQSNYK--QQSSVSVTLGPTVDEIRLNCVMFFMDQFWLKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVC---------------KYSN-----IEN--CTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFKRDSPSISTTDNK--------------IFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIH-SKITELECLIESNPSPQISKINWLFFSSYNYFDENFDE----IPKKYSCNQG-HWKNKIMKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVA 2875
R+ QS CT + ++ IQCF +P +Y V L T+ M CV+ NQHGKVQW+A LLGYDR I G RYR++GD +GEH LQI +V +ED G+YECQV P+ HP LRR T L +++ P +P LY L Q++ +S D I + C+S G P SF W +N + + +SL S+L + + +K + C+VTN+AT+ + P+ + L + + TPP P I S + V LE + +K C +PPG P L+W W ++N Y+ ++ + L++T+ K+K +++L + I++++H L C HP G+ + I +K+PP + I+ID + + + + ++ ++ I C TG YY +KW G ++L K Q Y Q+S V+VT+ D ++C ++ + + V + I P + + Y+S ++ L+L C+++GG P P+L W + + I+ + + + +S+ ++ Q+ N C +R + + S+ + L V+FPP +I I + P + +V NC SNP + + W C K SN EN C + E G G + S L L W YH+ + C + + PV ++QL I+F P F+ + ++ ++ + + S+ + ANP W +D K + ++ Y ++ATN +S +F +++TY ++G + + + EC E+NP+ I W F + E+ E + CN+ + KLFV+C+ S ++S L +Y + +DVGTY+C ++ K + + P I +P + K A
Sbjct: 95 GRVIQSSECTPQERQNRIQCFDKLPEDKYAVSLGNTVNMQCVVLNQHGKVQWRAKKILLGYDRSIPGYQRYRVIGDVGRGEHTLQIMDVQLEDAGEYECQVTPVPANNHPLLRRKTELIVLIKPSVPSILYPDVPPLDRQLI-ISKPDPILRITVLCVSVGGSPPPSFSWSLNNQEIPQIRTTPSPRRMSLVQNPWMKLEPVIETQPETGQSRLTLLKSGLKDGDQLMCSVTNAATQLH-HDPHQRNLSTHVTIRVHTPPGPPRIISPETDHVYLE---GDELKAICIASPPGNPLGGLFWRWLLHPVQVNDPSIGHVSGLGGRGGARLSDYSSVEAYLHAQDVGYLPGEKSGTTSPHQTLTPISEDVQPLHYTLNKDKDQLVNTLVIPRITRRYHASKLVCETGHPVGTAHQTSINIYVKHPPANVTISIDGGTVRQEIAAGRREYVVYGRAGEMKTIICRTGPYYRDAQIKWVAQGPDPGLLKAPRELFGQTKTIQTSDGYSRVQESRVNVTITEADDRGYIDCRVWGEGDTRMDARVRLDIIYPPTKPTVSGYKSLDPIKMAHTLELVCITYGGNPPPELAWFKDNQQILSTSDVVVLGRQSSLKLKVIPQKEDNGAHYHCSSRNAATGPEGIRSESVILNVLFPPERIVISFHSPPVVSSGHQLVINCQADTSNPVATISWWHFRCGPAHAFTQSLVSSGQKKSNAGRTPTENTKCKSVAMQGTNEKPTPGAHG-GLQANSRLWLRPTWHYHMDYIECRT--ENPEYGPPVWHDRLQLNITFAPQFLGLQAGTEKVVR-EGQSMSLDLGPYANPPVTAVNWFINGVPLLFEPSDTKEVEGVFASGRNGELLALHRIKRKHMANYTVMATNPEAESKAVFFLNVTYPAELIGSTVDNITDSVNGVASFECTAEANPAVAIRSFTWYRFVPPKSWPEDSHEAALQLGSPIPCNESTSAPGRTDKLFVRCQQESQLRMSSRLSLYRVTEDDVGTYVCEVNNGLGEPVQKRVNFLHPFPPTVIRLPRYTKAA 1117
BLAST of NPHS1-6 vs. TrEMBL
Match: G7YEL7 (Nephrin OS=Clonorchis sinensis OX=79923 GN=CLF_106049 PE=4 SV=1) HSP 1 Score: 337.035 bits (863), Expect = 2.683e-91 Identity = 260/1035 (25.12%), Postives = 441/1035 (42.61%), Query Frame = 2
Query: 146 LENSQEARIKQSDNCTQDDIKDNIQCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIK-HPALRRITHLTIIVLPEIPK-LYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNG-----KPVCSAVK-------------ICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQ-TLKVIIQ--TPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLN--------------------YTKIK----------------------------IDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNS---IYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRG-----KDLRSVEKIWQIQSN--YKQQSSVSVTLGPTVDEIRLNCVMFFMDQFWLKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNP------------PSGLIWKIKVCKYSNIE-----------NCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFK------RDSPSISTT--------DNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVG--ERFMKLPIHSKITELECLIESNPSPQISKINWLFFSSYNYFDENFDEIPKKYS----CNQGHWKNKI-MKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVA 2875
L+ S AR+ QS CT + +D IQCF ++P +Y V L T+ M CV+ NQHGKVQW+A LLGYDR + G PRYRI+GD +G+H LQI +V +ED G+YECQV P+ HP LRR T L ++V P +P+ LY NQ++ +S D I + C++ G P SF W +N KP+ ++ I E+ S+L + +K ++C VTN+AT+ + T +V+I+ TPP P I + E E E +K C +PPG P L+W W ++ Y+ ++ + L++T+ K K I++L + I++++H L C HP GS ++I +KY P + I+++ + + ++ ++ ++ + C T Y+ N+KW G + L KI + + Q+S V V + D ++C ++ + + V + I P I Y++ ++ L++ C++ GG P P+L W + + + SE L +S+ +L QR N C R + S+ + L VVFPP + + + P+ + + +V +C SNP P+ + K K + E C L +E GT G K +S L L W YH+ + C + + PV ++QL I+F P F+ + + ++ + ++ + +NP W + +P+ + T + K+ + Y ++A N + +S IF +++T+ + G E + ++T L C +NPS I W + + ++ +E + S CNQ + K F+ C+ + S IY + +DVGTY+C ++ K I + P + +P + K A
Sbjct: 34 LQVSDAARVIQSSACTPQERQDRIQCFDVLPQDQYSVPLGNTVNMQCVVLNQHGKVQWRAKKILLGYDRSLPGYPRYRIIGDVGRGQHTLQIMDVQLEDAGEYECQVTPVPANNHPLLRRKTELVVLVKPSVPRILYPDVPPPDNQLI-ISQPDPIVRITVLCVTVGGSPPPSFTWIINNQEIPTKPLQTSAGRHPDRGPWAKLDPIINTET-EKQESRLSLLKAGLKDGDQLTCGVTNAATQLHHDIQQRNLTTQVMIRVHTPPGPPRI--ISPETKYEYLEGEELKAVCVASPPGNPFGGLFWRWLLQPAQVGDQKTGRIIGFGGRGGARMNEYSAVEAYLKTAEHLGSQSADEMSQGMSDLISEDVQPLHYTLNKEKDQLINTLVIPRITRRYHAAKLICETGHPVGSAHQTSIEIFVKYAPANVTISVEGGSVREELVSGRRESVVYGQAGEIKTLICRTSPYFKQANIKWITQSSDSGPMKPPRQLFGQTKIMRTPDGKSWLQESRVDVKISDEDDRGYVDCRVWSEGDLRVDARVRMDIIYPPGVPVITGYRNLQPVKMAHSLEMTCVTSGGNPPPELAWFRDKQQLPNSEEVILLGRQSSLKLKLVPQRDDNGAHYHCAARNAATGNVGIASEAVVLNVVFPPQGVSVNFHGPSVVPSGQPLVISCQADSSNPVAIINWWHFRCGPAHAFSQFKASKTTVDEVLYERRTPERAKCKSEKLEGTEENPVPGTAG-GLKAQSRLWLRPTWHYHMDYIECRT--ENPEYGPPVWHDRLQLNITFAPQFLGLQTRKEHVIR-EGTTVNLDLGPYSNPPVTSVSWFVEGRPIPPETAPAAARTGVHTSGRLGELLALHKISRTHMANYTVVARNDIAESSAIFFLNVTFPASLAGAAEENVTQTTSDQVT-LVCQATANPSIAIQSFTWYRYVPPPGWPQDVNEAEVQLSAPMPCNQSDAGPSVDDKYFIHCQRDDSVHMTSRFSIYRINRDDVGTYVCEINNGLGDPVRKNIHFVYHFAPGIVRLPRYTKAA 1059
BLAST of NPHS1-6 vs. TrEMBL
Match: A0A4S2LBR4 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008491 PE=4 SV=1) HSP 1 Score: 335.88 bits (860), Expect = 6.984e-91 Identity = 259/1030 (25.15%), Postives = 437/1030 (42.43%), Query Frame = 2
Query: 164 ARIKQSDNCTQDDIKDNIQCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIK-HPALRRITHLTIIVLPEIPK-LYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNG-----KPVCSAVK-------------ICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQ-TLKVIIQ--TPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLN--------------------YTKIK----------------------------IDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNS---IYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRG-----KDLRSVEKIWQIQSN--YKQQSSVSVTLGPTVDEIRLNCVMFFMDQFWLKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVC-----------------------KYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNF-SQILLTLDSLNQSIIVKTVANPLPIRFKWLFK------RDSPSISTT--------DNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVG--ERFMKLPIHSKITELECLIESNPSPQISKINWLFFSSYNYFDENFDEIPKKYS----CNQGHWKNKI-MKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVA 2875
AR+ QS CT + +D IQCF ++P +Y V L T+ M CV+ NQHGKVQW+A LLGYDR + G PRYRI+GD +G+H LQI +V +ED G+YECQV P+ HP LRR T L ++V P +P+ LY NQ++ +S D I + C + G P SF W +N KP+ ++ I E+ S+L + +K ++C+VTN+AT+ + T +V+I+ TPP P I + E E E +K C +PPG P L+W W ++ Y+ ++ + L++T+ K K I++L + I++++H L C HP GS ++I +KY P + I++D + + ++ ++ ++ + C T Y+ N+KW G + L KI + + Q+S V V + D ++C ++ + + V + I P I Y+S ++ L++ C++ GG P P+L W + + + +E L +S+ +L QR N C R + S + L VVFPP + + + P+ + + +V +C SNP + + W C + S C L +E GT G K +S L L W YH+ + C + + PV ++QL I+F P F+ + Q++ ++N + + +NP W + P+ + T + K+ + Y ++A N + +S +F +++T+ + G E + ++T L C +NPS W + + ++ +E + S CNQ + K F+ C+ + S L IY + +DVGTY+C ++ K I + P+ + +P + K A
Sbjct: 116 ARVIQSSACTPRERQDRIQCFDVLPQDQYSVPLGNTVNMQCVVLNQHGKVQWRAKKILLGYDRSLPGYPRYRIIGDVGRGQHTLQIMDVQLEDAGEYECQVTPVPANNHPLLRRKTELVVLVKPSVPRILYPDVPPPDNQLI-ISQPDPIVRITVLCATVGGSPAPSFTWIINNQEIPMKPLQASAGRHPDRGPWAKLDPIINTET-EKQESRLSLLKAGLKDGDQLTCSVTNAATQLHHDIQQRNLTTQVVIRVHTPPGPPRI--ISPETKYEYLEGEELKAVCVASPPGNPFGGLFWRWLLQPSQVGDQKTGRIIGFGGRGGARMNEYSDVEAYLKTAEHLGSQSADEMSQGMSDLISEDVQPLHYTLNKEKDQLINTLVIPRITRRYHAAKLICETGHPVGSAHQTSIEIFVKYAPANVTISVDGGSVREELVSGRRESVVYGQAGEIKTLICRTSPYFKQANIKWITQSSDSGPMKPPRQLFGQTKIMRTPDGKSWLQESRVDVKISEEDDRGYVDCRVWSEGDLRVDARVRMDIIYPPGVPVITGYRSLQPVKMAHSLEMTCVTSGGNPPPELAWFRDKQQLPNAEEVILLGRQSSLKLKLVPQRDDNGAHYHCAARNAATGNVGIASDAVVLNVVFPPQGVSVNFHGPSVVPSGQPLVISCQADSSNPVATINWWHFRCGPAHAFSQFKASKTTIEEALYDRRTSERAKCKSEKLEGTEENPVPGTA-GGLKAQSRLWLRPTWHYHMDYIECRT--ENPEYGPPVWHDRLQLNITFAPQFLGLQTRKEQVIREGTTVN--LDLGPYSNPPVTSVSWFVEGRPIPPETPPAAARTGVHTSGRLGELLALHKISRTHMANYTVVARNDIAESSAVFFLNVTFPASLAGAAEENVTQTTSDQVT-LVCQATANPSIATQAFTWYRYVPPPGWPQDVNEAEVQLSAPMPCNQSDASPSVDDKYFIHCQRDDSIHMTSRLSIYRINRDDVGTYVCEINNGLGDPVRKNIHFVYHFTPSIVRLPRYTKAA 1135
BLAST of NPHS1-6 vs. TrEMBL
Match: A0A4S2LB20 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008491 PE=4 SV=1) HSP 1 Score: 335.495 bits (859), Expect = 8.096e-91 Identity = 268/1071 (25.02%), Postives = 453/1071 (42.30%), Query Frame = 2
Query: 41 CLILEICENVSLNSNTTLFSINSRNFKTLNELARDLENSQEARIKQSDNCTQDDIKDNIQCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIK-HPALRRITHLTIIVLPEIPK-LYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNG-----KPVCSAVK-------------ICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQ-TLKVIIQ--TPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLN--------------------YTKIK----------------------------IDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNS---IYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRG-----KDLRSVEKIWQIQSN--YKQQSSVSVTLGPTVDEIRLNCVMFFMDQFWLKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVC-----------------------KYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNF-SQILLTLDSLNQSIIVKTVANPLPIRFKWLFK------RDSPSISTT--------DNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVG--ERFMKLPIHSKITELECLIESNPSPQISKINWLFFSSYNYFDENFDEIPKKYS----CNQGHWKNKI-MKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVA 2875
C+ C ++ +S T F S F L +D ++ AR+ QS CT + +D IQCF ++P +Y V L T+ M CV+ NQHGKVQW+A LLGYDR + G PRYRI+GD +G+H LQI +V +ED G+YECQV P+ HP LRR T L ++V P +P+ LY NQ++ +S D I + C + G P SF W +N KP+ ++ I E+ S+L + +K ++C+VTN+AT+ + T +V+I+ TPP P I + E E E +K C +PPG P L+W W ++ Y+ ++ + L++T+ K K I++L + I++++H L C HP GS ++I +KY P + I++D + + ++ ++ ++ + C T Y+ N+KW G + L KI + + Q+S V V + D ++C ++ + + V + I P I Y+S ++ L++ C++ GG P P+L W + + + +E L +S+ +L QR N C R + S + L VVFPP + + + P+ + + +V +C SNP + + W C + S C L +E GT G K +S L L W YH+ + C + + PV ++QL I+F P F+ + Q++ ++N + + +NP W + P+ + T + K+ + Y ++A N + +S +F +++T+ + G E + ++T L C +NPS W + + ++ +E + S CNQ + K F+ C+ + S L IY + +DVGTY+C ++ K I + P+ + +P + K A
Sbjct: 4 CITSSYCFHLPFSSLTMQF--GSLMFVATIGLLQDSVDA--ARVIQSSACTPRERQDRIQCFDVLPQDQYSVPLGNTVNMQCVVLNQHGKVQWRAKKILLGYDRSLPGYPRYRIIGDVGRGQHTLQIMDVQLEDAGEYECQVTPVPANNHPLLRRKTELVVLVKPSVPRILYPDVPPPDNQLI-ISQPDPIVRITVLCATVGGSPAPSFTWIINNQEIPMKPLQASAGRHPDRGPWAKLDPIINTET-EKQESRLSLLKAGLKDGDQLTCSVTNAATQLHHDIQQRNLTTQVVIRVHTPPGPPRI--ISPETKYEYLEGEELKAVCVASPPGNPFGGLFWRWLLQPSQVGDQKTGRIIGFGGRGGARMNEYSDVEAYLKTAEHLGSQSADEMSQGMSDLISEDVQPLHYTLNKEKDQLINTLVIPRITRRYHAAKLICETGHPVGSAHQTSIEIFVKYAPANVTISVDGGSVREELVSGRRESVVYGQAGEIKTLICRTSPYFKQANIKWITQSSDSGPMKPPRQLFGQTKIMRTPDGKSWLQESRVDVKISEEDDRGYVDCRVWSEGDLRVDARVRMDIIYPPGVPVITGYRSLQPVKMAHSLEMTCVTSGGNPPPELAWFRDKQQLPNAEEVILLGRQSSLKLKLVPQRDDNGAHYHCAARNAATGNVGIASDAVVLNVVFPPQGVSVNFHGPSVVPSGQPLVISCQADSSNPVATINWWHFRCGPAHAFSQFKASKTTIEEALYDRRTSERAKCKSEKLEGTEENPVPGTA-GGLKAQSRLWLRPTWHYHMDYIECRT--ENPEYGPPVWHDRLQLNITFAPQFLGLQTRKEQVIREGTTVN--LDLGPYSNPPVTSVSWFVEGRPIPPETPPAAARTGVHTSGRLGELLALHKISRTHMANYTVVARNDIAESSAVFFLNVTFPASLAGAAEENVTQTTSDQVT-LVCQATANPSIATQAFTWYRYVPPPGWPQDVNEAEVQLSAPMPCNQSDASPSVDDKYFIHCQRDDSIHMTSRLSIYRINRDDVGTYVCEINNGLGDPVRKNIHFVYHFTPSIVRLPRYTKAA 1060
BLAST of NPHS1-6 vs. Ensembl Cavefish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1]) HSP 1 Score: 129.798 bits (325), Expect = 8.184e-30 Identity = 172/818 (21.03%), Postives = 305/818 (37.29%), Query Frame = 2
Query: 251 VVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIESLSLSVS--KLIFVPNNIK---TIQSISCAVT------NSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLD-----ENVVQKINCITGEYYGSVNLKWKMD---------DLSRGKDLRSVEKIWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMFFMDQFWLKSTVTIMKIKRP-ERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICE---SLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFKRDSPSISTTDNKIFFQK--------VDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKI---TELECLIESNP-SPQISKINWL 2581
V T + C + G VQW + LLG R + G PRY + GD +G++ LQI++V +EDD YECQV P + R L + + P P YF+ + + + +TCI+ +P Y +G ++ +IES + S S KL +K + I +T + + + PP P I+ ++ E+ S++L C + G P A+L+W TKN ++ + +S+K G V G+V + P+ I T L +++ L E + C T V+++W + +S G++ S+++ T+ + + L C F + + + P ++++++ +L ++L C S GG P L W+ N +T+ + S +F L +Q N +C+ E K + S IKL V FP + +KI L + +C+ SNP + + W + + + +E + +F G S L L + E+H + C+ SL S N++ V L + F P F ++ D+ I+V ANP +WLF + + D++ F + V Y++ +N+ G I +D+ YSP + + P+ + +L C ++NP +P + W+
Sbjct: 53 TVRTGATALLKCEVLRASGSVQWVKDGLLLGPQRSLPGHPRYSMTGDQDRGQYHLQIEDVRLEDDSPYECQVGRSKTSRPIVSRTVWLNVQIPPAEP--YFEWDAGKPWV-------EGEEYTVTCIAPDAKPAADVLIYKDGD------ELTDIESFTESGSEDKLQNTHAEVKYRNVMIYIGLCLTALFLYLPPSLLLPLLLRYLSLGLAVSEVPPQAPVIEGLRSQ---EVKAGTSLRLVC-LSYGGNPLATLHW-----------------------TKNGEVLSTNWEVDVVSQKASSGLNMQVQPEDNGAVLCCESVNQVSRSPQSITRT-----LTVLFEPAEVTLLGSFEAVEGNQVTLCCHTSSSNPPVHIRWWLGFKELNNATVTISEGEN-----------GGMMTMSNLTYTVSREENSLPLTCEAFNRGTRYSSIKSQPLSVYYPPQKVWLDAPPEGTLLRSGTTVRLVCFSSGGNPTGRLTWMKNNKTVQVPSKHVESERGVSREFLLTLQPSDNLATYRCDATNE---AKEIRSAQIKLLVQFPAVSVKILVK-QKELRAGRSLSLDCLAGSSNPKANISWSLGSTRLNGVEQAPKR------------AEFGGLSVSSKLTLPLGSEHHGKRVSCQAFSSLLSEGVNTFYV------LNVLFPPEFAEDQPSEVQIVEDDTATLPIMVS--ANPEDFTCEWLFHGEK-VVKDRDHRYHFPEGWALEIWNVTRRDAGNYRVECSNAEGKRSSIIKLDVQYSPTV---KMKTDPVFVNVGDTADLMCEADANPITPGMFSWKWM 784
BLAST of NPHS1-6 vs. Ensembl Cavefish
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:HGNC Symbol;Acc:HGNC:23204]) HSP 1 Score: 99.7525 bits (247), Expect = 7.418e-21 Identity = 69/208 (33.17%), Postives = 96/208 (46.15%), Query Frame = 2
Query: 245 EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAV---KICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVII 859
+ VV Q + + C I HG V W + LG R + G PRY +VGD S GEH L+I+ V + DD +ECQ I+ R LT++V PE P + IV L D P+ LTC + + +P S W NG+ + A+ + +VS L P+NI+T Q I C +N A PN + V I
Sbjct: 8 DLVVVAGQPVTLPCSIPGYHGVVLWIKDGLALGVGRDLSGYPRYTVVGDHSSGEHHLRIQRVELMDDAVFECQA----IQAAMRSRPARLTVLVPPEDPVID------GGPIVSLRAGD---PLNLTCHADNAKPAASIIWIRNGEVLNGAMYSKTLLRDGRRESTVSTLYISPSNIETGQKIICRASNK------AIPNGKETSVTI 196
BLAST of NPHS1-6 vs. Ensembl Cavefish
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:HGNC Symbol;Acc:HGNC:23204]) HSP 1 Score: 99.7525 bits (247), Expect = 7.880e-21 Identity = 69/208 (33.17%), Postives = 96/208 (46.15%), Query Frame = 2
Query: 245 EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAV---KICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVII 859
+ VV Q + + C I HG V W + LG R + G PRY +VGD S GEH L+I+ V + DD +ECQ I+ R LT++V PE P + IV L D P+ LTC + + +P S W NG+ + A+ + +VS L P+NI+T Q I C +N A PN + V I
Sbjct: 8 DLVVVAGQPVTLPCSIPGYHGVVLWIKDGLALGVGRDLSGYPRYTVVGDHSSGEHHLRIQRVELMDDAVFECQA----IQAAMRSRPARLTVLVPPEDPVID------GGPIVSLRAGD---PLNLTCHADNAKPAASIIWIRNGEVLNGAMYSKTLLRDGRRESTVSTLYISPSNIETGQKIICRASNK------AIPNGKETSVTI 196
BLAST of NPHS1-6 vs. Ensembl Cavefish
Match: kirrel3a (kirre like nephrin family adhesion molecule 3a [Source:ZFIN;Acc:ZDB-GENE-091117-44]) HSP 1 Score: 97.4413 bits (241), Expect = 3.965e-20 Identity = 80/267 (29.96%), Postives = 126/267 (47.19%), Query Frame = 2
Query: 221 CFLIVPFP-EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAV---KICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIK-SFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWS 1006
C + V P + VV Q + + C I+ HG V W + LG +R + G PRY ++GD SKGE+ L I+ + DDG +ECQ GI+ R LT++V P+ P + + IV L D P+ LTC + + +P S W NG+ + A+ + +VS L +NI++ Q I+C +N A PN + V I ++P + S + +LE ++ +K HC+ P + Y RW+
Sbjct: 28 CAVFVQQPHDVVVVAGQPVTLPCSITGFHGMVLWDGLA--LGVNRDLSGYPRYDVIGDHSKGEYHLLIQRTELGDDGSFECQ----GIQAGIRSRPARLTVLVPPDDPVI------IGAPIVSLRAGD---PLNLTCHADNAKPAASIIWIRNGEVLNGAMYSKTLLRDGQRESTVSTLYISASNIESGQRITCRASNKAA------PNGKEATVTIDI-QHLPLVNLSVEPQPILEGNL---VKF--HCSAKANPPVTHY---RWA 264
BLAST of NPHS1-6 vs. Ensembl Cavefish
Match: kirrel3l (kirre like nephrin family adhesion molecule 3, like [Source:ZFIN;Acc:ZDB-GENE-070831-1]) HSP 1 Score: 92.0485 bits (227), Expect = 2.028e-18 Identity = 61/208 (29.33%), Postives = 95/208 (45.67%), Query Frame = 2
Query: 245 EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAV---KICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVII 859
+ VV Q++ + CVI G VQW + LG +R + G RY ++GDP+ GEH L I++ + DD YECQ G++ R LT++V P P + +V L P LTC + +P WY +G+ + +A+ + E +VS L P + + ++ +C V N PA P + V I
Sbjct: 21 DQVVVAGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWTRYSLLGDPASGEHSLVIESSELADDAVYECQATQAGLRSHRAR----LTVLVPPSDPVVD------GGPVVRLKAH---TPHNLTCRATGAKPAAEITWYRDGEVMHTAIYSKSLMEDGKRETAVSLLPMTPEDRDSGRTYTCRVLN------PAAPAGRQTSVTI 209
BLAST of NPHS1-6 vs. Ensembl Medaka
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:NCBI gene;Acc:101170290]) HSP 1 Score: 100.138 bits (248), Expect = 6.668e-21 Identity = 79/262 (30.15%), Postives = 119/262 (45.42%), Query Frame = 2
Query: 245 EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAV-KICEIESL------SLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIK-SFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWS 1006
+ VV Q + + C I HG V W + LG R + G PRY ++GD GEH L+I+ V + DD +ECQ ++ R LT++V P+ P + +V L D P+ LTC + + +P S W NG+ + A+ IE+L +VS L P+NI+T Q I C +N A PN + V I ++P + S + VLE S+ + C+ P +LY RW+
Sbjct: 26 DLVVVAGQPVTLPCSIPGYHGIVLWIKDGLALGVGRDLSGYPRYTVIGDHGSGEHHLRIQRVELMDDAVFECQA----VQAAMRSRPARLTVLVPPDDPVIG------GGPVVSLRAGD---PLNLTCHADNAKPAASIIWMRNGEVLNGAMYSKVSIEALVRDGRRESTVSTLYLSPSNIETGQQIICRASNK------AVPNGKEASVTIDI-QHLPLVNLSVEPQAVLEGSL-----VRFQCSAKANPPVTLY---RWA 259
BLAST of NPHS1-6 vs. Ensembl Medaka
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:NCBI gene;Acc:101170290]) HSP 1 Score: 99.7525 bits (247), Expect = 9.579e-21 Identity = 76/258 (29.46%), Postives = 116/258 (44.96%), Query Frame = 2
Query: 245 EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAV---KICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIK-SFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWS 1006
+ VV Q + + C I HG V W + LG R + G PRY ++GD GEH L+I+ V + DD +ECQ ++ R LT++V P+ P + +V L D P+ LTC + + +P S W NG+ + A+ + +VS L P+NI+T Q I C +N A PN + V I ++P + S + VLE S+ + C+ P +LY RW+
Sbjct: 22 DLVVVAGQPVTLPCSIPGYHGIVLWIKDGLALGVGRDLSGYPRYTVIGDHGSGEHHLRIQRVELMDDAVFECQA----VQAAMRSRPARLTVLVPPDDPVIG------GGPVVSLRAGD---PLNLTCHADNAKPAASIIWMRNGEVLNGAMYSKALVRDGRRESTVSTLYLSPSNIETGQQIICRASNK------AVPNGKEASVTIDI-QHLPLVNLSVEPQAVLEGSL-----VRFQCSAKANPPVTLY---RWA 251
BLAST of NPHS1-6 vs. Ensembl Medaka
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:NCBI gene;Acc:101170290]) HSP 1 Score: 99.3673 bits (246), Expect = 1.205e-20 Identity = 76/258 (29.46%), Postives = 116/258 (44.96%), Query Frame = 2
Query: 245 EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAV---KICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIK-SFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWS 1006
+ VV Q + + C I HG V W + LG R + G PRY ++GD GEH L+I+ V + DD +ECQ ++ R LT++V P+ P + +V L D P+ LTC + + +P S W NG+ + A+ + +VS L P+NI+T Q I C +N A PN + V I ++P + S + VLE S+ + C+ P +LY RW+
Sbjct: 54 DLVVVAGQPVTLPCSIPGYHGIVLWIKDGLALGVGRDLSGYPRYTVIGDHGSGEHHLRIQRVELMDDAVFECQA----VQAAMRSRPARLTVLVPPDDPVIG------GGPVVSLRAGD---PLNLTCHADNAKPAASIIWMRNGEVLNGAMYSKALVRDGRRESTVSTLYLSPSNIETGQQIICRASNK------AVPNGKEASVTIDI-QHLPLVNLSVEPQAVLEGSL-----VRFQCSAKANPPVTLY---RWA 283
BLAST of NPHS1-6 vs. Ensembl Medaka
Match: kirrel3l (kirre like nephrin family adhesion molecule 2 [Source:NCBI gene;Acc:101173407]) HSP 1 Score: 99.3673 bits (246), Expect = 1.209e-20 Identity = 79/278 (28.42%), Postives = 121/278 (43.53%), Query Frame = 2
Query: 245 EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIK-HPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAV---KICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVII--QTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTM 1060
+ VV Q++ + CVI G VQW + LG +R + G RY ++GDP GEH L I + + DD YECQ G++ H A LT++V P P + +V L P LTC + +P WY +G+ + +A+ + + +VS L +P + T ++ +C V N PA P + V I Q PP+V V +V E K+ C+ P + Y RWS + ++ D L T+
Sbjct: 25 DQVVVSGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWTRYSLMGDPLSGEHSLLIDSAELADDAVYECQATQAGLRSHRA-----KLTVLVPPSDPVVE------GGPVVRLKAH---TPHNLTCRASGAKPAAEITWYRDGEVMETAIYSKTLMQDGKRETAVSMLPIIPEDSDTGRTYTCRVLN------PAAPAGRQTSVTINVQHPPSV-------TLSVQPQTVTEGAKVLFLCSASANPEITGY---RWSKGGVPISEANGDSLEVTV 272
BLAST of NPHS1-6 vs. Ensembl Medaka
Match: kirrel3l (kirre like nephrin family adhesion molecule 2 [Source:NCBI gene;Acc:101173407]) HSP 1 Score: 99.3673 bits (246), Expect = 1.264e-20 Identity = 79/278 (28.42%), Postives = 121/278 (43.53%), Query Frame = 2
Query: 245 EYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIK-HPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAV---KICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVII--QTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTM 1060
+ VV Q++ + CVI G VQW + LG +R + G RY ++GDP GEH L I + + DD YECQ G++ H A LT++V P P + +V L P LTC + +P WY +G+ + +A+ + + +VS L +P + T ++ +C V N PA P + V I Q PP+V V +V E K+ C+ P + Y RWS + ++ D L T+
Sbjct: 26 DQVVVSGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWTRYSLMGDPLSGEHSLLIDSAELADDAVYECQATQAGLRSHRA-----KLTVLVPPSDPVVE------GGPVVRLKAH---TPHNLTCRASGAKPAAEITWYRDGEVMETAIYSKTLMQDGKRETAVSMLPIIPEDSDTGRTYTCRVLN------PAAPAGRQTSVTINVQHPPSV-------TLSVQPQTVTEGAKVLFLCSASANPEITGY---RWSKGGVPISEANGDSLEVTV 273
BLAST of NPHS1-6 vs. Planmine SMEST
Match: SMESG000056135.1 (SMESG000056135.1) HSP 1 Score: 2080.45 bits (5389), Expect = 0.000e+0 Identity = 1019/1025 (99.41%), Postives = 1021/1025 (99.61%), Query Frame = 2
Query: 17 MLFLFYCWCLILEICENVSLNSNTTLFSINSRNFKTLNELARDLENSQEARIKQSDNCTQDDIKDNIQCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRGKDLRSVEKIWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMFFMDQFWLKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFKRDSPSISTTDNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKITELECLIESNPSPQISKINWLFFSSYNYFDENFDEIPKKYSCNQGHWKNKIMKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVAILLNTRSLAKAFMHSGLTSNITLHIELHLNKSVLFPVKVNRINSGIYLVTSEFSYQILEDIEKIYLGLKTKL 3091
MLFLFYCWCLILEICENVSLNSNTTLFSINSRNFKTLNELARDLENSQEARIKQSDNCTQDDIKDNIQCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRGKDLRSVEKIWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMFFMDQFWLKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFKRDSPSISTTDNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKITELECLIESNPSPQISKINWLFFSSYNYFDENFDEIPKKYSCNQGHWKNKIMKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVAILLNTRSLAKAFMHSGLTSNITLHIELHLNKSVLFPVKVNRINSGIYLVTSEFSYQILEDIEKIY + K+
Sbjct: 1 MLFLFYCWCLILEICENVSLNSNTTLFSINSRNFKTLNELARDLENSQEARIKQSDNCTQDDIKDNIQCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRGKDLRSVEKIWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMFFMDQFWLKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFKRDSPSISTTDNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKITELECLIESNPSPQISKINWLFFSSYNYFDENFDEIPKKYSCNQGHWKNKIMKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVAILLNTRSLAKAFMHSGLTSNITLHIELHLNKSVLFPVKVNRINSGIYLVTSEFSYQILEDIEKIYFRAENKI 1025 HSP 2 Score: 441.81 bits (1135), Expect = 1.594e-133 Identity = 233/234 (99.57%), Postives = 234/234 (100.00%), Query Frame = 1
Query: 3070 FRAENKIGSSSLMLEAVSESIPDPPIVHEIKFLQPNSLFVKIIQTFDGGYPQSFQVFWKEANRNTTENKMWEVPGDYTKKFIIIKISDTFLSKNYEIQVCSKNLVYGRCKVCPKKFLDLNGKQAHEPNSEDFNENLYELIFQFNLKIVISVAGXXXXXXXXXXXXXXXXXKCKHNHWNSERRPTVNHSLKYGNDLLIPNLSITDSKSDFQNITSFDKTGIPLNPIITHCSQSKV 3771
FRAENKIGSSSLMLEAVSESIPDPPIVHEIKFLQPNSLFVKIIQTFDGGYPQSFQVFWKEANRNTTENKMWEVPGDYTKKFIIIKISDTFLSKNYEIQVCSKNLVYGRCKVCPKKFLDLNGKQAHEPNSEDFNENLYELIFQFNLKIVISVAGFVFGLILLAIFIIIFLFKCKHNHWNSERRPTVNHSLKYGNDLLIPNLSITDSKSDFQNITSFDKTGIPLNPI+THCSQSKV
Sbjct: 1019 FRAENKIGSSSLMLEAVSESIPDPPIVHEIKFLQPNSLFVKIIQTFDGGYPQSFQVFWKEANRNTTENKMWEVPGDYTKKFIIIKISDTFLSKNYEIQVCSKNLVYGRCKVCPKKFLDLNGKQAHEPNSEDFNENLYELIFQFNLKIVISVAGFVFGLILLAIFIIIFLFKCKHNHWNSERRPTVNHSLKYGNDLLIPNLSITDSKSDFQNITSFDKTGIPLNPILTHCSQSKV 1252
BLAST of NPHS1-6 vs. Planmine SMEST
Match: SMESG000056135.1 (SMESG000056135.1) HSP 1 Score: 1972.59 bits (5109), Expect = 0.000e+0 Identity = 977/1025 (95.32%), Postives = 984/1025 (96.00%), Query Frame = 2
Query: 17 MLFLFYCWCLILEICENVSLNSNTTLFSINSRNFKTLNELARDLENSQEARIKQSDNCTQDDIKDNIQCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLKVIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRGKDLRSVEKIWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMFFMDQFWLKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFKRDSPSISTTDNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKITELECLIESNPSPQISKINWLFFSSYNYFDENFDEIPKKYSCNQGHWKNKIMKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVAILLNTRSLAKAFMHSGLTSNITLHIELHLNKSVLFPVKVNRINSGIYLVTSEFSYQILEDIEKIYLGLKTKL 3091
MLFLFYCWCLILEICENVSLNSNTTLFSINSRNFKTLNELARDLENSQEARIKQSDNCTQDDIKDNIQCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIE + KL C T S + P++ I+ PPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRGKDLRSVEKIWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMFFMDQFWLKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFKRDSPSISTTDNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKITELECLIESNPSPQISKINWLFFSSYNYFDENFDEIPKKYSCNQGHWKNKIMKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVAILLNTRSLAKAFMHSGLTSNITLHIELHLNKSVLFPVKVNRINSGIYLVTSEFSYQILEDIEKIY + K+
Sbjct: 1 MLFLFYCWCLILEICENVSLNSNTTLFSINSRNFKTLNELARDLENSQEARIKQSDNCTQDDIKDNIQCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIEKHFMCRYKL--------------CHPTISGISK----PSN------IKAPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWRWSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNSIYKNKNYIFLDENVVQKINCITGEYYGSVNLKWKMDDLSRGKDLRSVEKIWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMFFMDQFWLKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVVFNCVTTESNPPSGLIWKIKVCKYSNIENCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKWLFKRDSPSISTTDNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKITELECLIESNPSPQISKINWLFFSSYNYFDENFDEIPKKYSCNQGHWKNKIMKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVAILLNTRSLAKAFMHSGLTSNITLHIELHLNKSVLFPVKVNRINSGIYLVTSEFSYQILEDIEKIYFRAENKI 1001 HSP 2 Score: 441.039 bits (1133), Expect = 1.932e-133 Identity = 234/238 (98.32%), Postives = 235/238 (98.74%), Query Frame = 1
Query: 3058 RKNLFRAENKIGSSSLMLEAVSESIPDPPIVHEIKFLQPNSLFVKIIQTFDGGYPQSFQVFWKEANRNTTENKMWEVPGDYTKKFIIIKISDTFLSKNYEIQVCSKNLVYGRCKVCPKKFLDLNGKQAHEPNSEDFNENLYELIFQFNLKIVISVAGXXXXXXXXXXXXXXXXXKCKHNHWNSERRPTVNHSLKYGNDLLIPNLSITDSKSDFQNITSFDKTGIPLNPIITHCSQSKV 3771
K FRAENKIGSSSLMLEAVSESIPDPPIVHEIKFLQPNSLFVKIIQTFDGGYPQSFQVFWKEANRNTTENKMWEVPGDYTKKFIIIKISDTFLSKNYEIQVCSKNLVYGRCKVCPKKFLDLNGKQAHEPNSEDFNENLYELIFQFNLKIVISVAGFVFGLILLAIFIIIFLFKCKHNHWNSERRPTVNHSLKYGNDLLIPNLSITDSKSDFQNITSFDKTGIPLNPI+THCSQSKV
Sbjct: 991 EKIYFRAENKIGSSSLMLEAVSESIPDPPIVHEIKFLQPNSLFVKIIQTFDGGYPQSFQVFWKEANRNTTENKMWEVPGDYTKKFIIIKISDTFLSKNYEIQVCSKNLVYGRCKVCPKKFLDLNGKQAHEPNSEDFNENLYELIFQFNLKIVISVAGFVFGLILLAIFIIIFLFKCKHNHWNSERRPTVNHSLKYGNDLLIPNLSITDSKSDFQNITSFDKTGIPLNPILTHCSQSKV 1228
BLAST of NPHS1-6 vs. Planmine SMEST
Match: SMESG000028689.1 (SMESG000028689.1) HSP 1 Score: 432.18 bits (1110), Expect = 1.277e-129 Identity = 300/1008 (29.76%), Postives = 490/1008 (48.61%), Query Frame = 2
Query: 143 DLENSQEARIKQSDNCTQDDIKDNIQCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLK----VIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWR-------WSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNS--IYK---NKNYIFLDENVVQKINCITGEYYGSVNLKWKMD-DLSRGKDLRS-VEKIWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMFFMDQFW-----LKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVV-FNCVTTESNPPSGLIWKIKVCKYSNIE--------NCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKW------LFKRDSPSISTTDNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKITELECLIESNPS-PQISKINWLFFSSYNYFD-ENFDEIPK-KYSCNQGHWKNKI----MKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVAILLNTRSLAKAFMHSGLTSNITLHIELH---LNKSVLFPVKVNRINSGIYLV 3022
D E S A+IKQS CT ++I + IQCF +P P Y V + C++ NQ+GKVQW+A LLGYDR I GRP YR+VG KGEH L+I NV+++D G YECQVQP+G +HP LRR T L ++V P+ P+++++G + V +++ Q E TC+S +G+P+V F+W++N + +E L+ S S+L + K I C V+N AT + P ++L ++I TPP P+I K V+++I + C +P G PA SL W W+ ++N NYT I Y+ T +K +S+L + ++ K H L CVA +P S S V++ I + P + I++ + L+S I K N+N I+ + + I C T YY +KW + + D S VEKIW+ ++++ S++ + + D+ L+C Q W ++S V + PE+ I+ Y ++ N ++ C + GG P P+LQW + + I S ++ + N V C+ + ++ + S + L V+FPP+ I ++ + +EN ++ C ESNPP+ + WK+ C E C E +LS G +F G +S ++ +W+ HL + CE + V+R +IQ+ ++F P F I N++ + +Q++ ++ +ANP + W L +++ SIST+ K+ + Y L+ N G+S + ++ITY P I+G++F + I + ELEC ++SNP+ P +S Y D + D +P CNQ WK K F KC + S + S LIIY+ DVG Y C ++ KTIEL P +S+P F+K A L ++ S + +T + ++ + + + + R +G Y+
Sbjct: 34 DSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQD-PERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDC------QAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLK-SLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTING-KFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYN--FYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVA 1030 HSP 2 Score: 52.7582 bits (125), Expect = 1.277e-129 Identity = 45/136 (33.09%), Postives = 69/136 (50.74%), Query Frame = 1
Query: 3001 QFRNIFSDFRIQLSDFGR--HRKNLFRAENKIGSSSLMLEAVSESIPDPPIVHEIKFLQPNSLFVKIIQTFDGGYPQSFQVFWKEANRNTTENKMWEVPGDYTK---KFIIIKI-SDTFLSKNYEIQVCSKNLVYG 3390
+FR F ++++ + R H + +AEN GS S +E S PD P+ ++ + + + IQ FDGG Q+FQV WKE + T+ + +V D TK FII + SDT Y I + S+N +G
Sbjct: 1022 RFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSP-TSLYRYEDVASDDTKITLSFIITGLKSDTA----YLISINSRNSKHG 1152
BLAST of NPHS1-6 vs. Planmine SMEST
Match: SMESG000028689.1 (SMESG000028689.1) HSP 1 Score: 432.18 bits (1110), Expect = 1.423e-129 Identity = 300/1008 (29.76%), Postives = 490/1008 (48.61%), Query Frame = 2
Query: 143 DLENSQEARIKQSDNCTQDDIKDNIQCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLK----VIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWR-------WSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNS--IYK---NKNYIFLDENVVQKINCITGEYYGSVNLKWKMD-DLSRGKDLRS-VEKIWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMFFMDQFW-----LKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVV-FNCVTTESNPPSGLIWKIKVCKYSNIE--------NCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKW------LFKRDSPSISTTDNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKITELECLIESNPS-PQISKINWLFFSSYNYFD-ENFDEIPK-KYSCNQGHWKNKI----MKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVAILLNTRSLAKAFMHSGLTSNITLHIELH---LNKSVLFPVKVNRINSGIYLV 3022
D E S A+IKQS CT ++I + IQCF +P P Y V + C++ NQ+GKVQW+A LLGYDR I GRP YR+VG KGEH L+I NV+++D G YECQVQP+G +HP LRR T L ++V P+ P+++++G + V +++ Q E TC+S +G+P+V F+W++N + +E L+ S S+L + K I C V+N AT + P ++L ++I TPP P+I K V+++I + C +P G PA SL W W+ ++N NYT I Y+ T +K +S+L + ++ K H L CVA +P S S V++ I + P + I++ + L+S I K N+N I+ + + I C T YY +KW + + D S VEKIW+ ++++ S++ + + D+ L+C Q W ++S V + PE+ I+ Y ++ N ++ C + GG P P+LQW + + I S ++ + N V C+ + ++ + S + L V+FPP+ I ++ + +EN ++ C ESNPP+ + WK+ C E C E +LS G +F G +S ++ +W+ HL + CE + V+R +IQ+ ++F P F I N++ + +Q++ ++ +ANP + W L +++ SIST+ K+ + Y L+ N G+S + ++ITY P I+G++F + I + ELEC ++SNP+ P +S Y D + D +P CNQ WK K F KC + S + S LIIY+ DVG Y C ++ KTIEL P +S+P F+K A L ++ S + +T + ++ + + + + R +G Y+
Sbjct: 15 DSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQD-PERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDC------QAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLK-SLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTING-KFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYN--FYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVA 1011 HSP 2 Score: 53.1434 bits (126), Expect = 1.423e-129 Identity = 45/136 (33.09%), Postives = 69/136 (50.74%), Query Frame = 1
Query: 3001 QFRNIFSDFRIQLSDFGR--HRKNLFRAENKIGSSSLMLEAVSESIPDPPIVHEIKFLQPNSLFVKIIQTFDGGYPQSFQVFWKEANRNTTENKMWEVPGDYTK---KFIIIKI-SDTFLSKNYEIQVCSKNLVYG 3390
+FR F ++++ + R H + +AEN GS S +E S PD P+ ++ + + + IQ FDGG Q+FQV WKE + T+ + +V D TK FII + SDT Y I + S+N +G
Sbjct: 1003 RFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSP-TSLYRYEDVASDDTKITLSFIITGLKSDTA----YLISINSRNSKHG 1133
BLAST of NPHS1-6 vs. Planmine SMEST
Match: SMESG000028689.1 (SMESG000028689.1) HSP 1 Score: 422.935 bits (1086), Expect = 8.046e-127 Identity = 300/1028 (29.18%), Postives = 490/1028 (47.67%), Query Frame = 2
Query: 143 DLENSQEARIKQSDNCTQDDIKDNIQCFLIVPFPEYVVELNQTIEMSCVISNQHGKVQWKANSDLLGYDRMIQGRPRYRIVGDPSKGEHVLQIKNVSIEDDGKYECQVQPIGIKHPALRRITHLTIIVLPEIPKLYFQGKHLSNQIVELSTKDQSKPIELTCISRHGRPEVSFEWYVNGKPVCSAVKICEIESLSLSVSKLIFVPNNIKTIQSISCAVTNSATRRYPAFPNHQTLK----VIIQTPPNVPEIKSFTNEKVLEISVNESIKLFCHCTPPGFPAASLYWEWR-------WSNEKLNYTKIKIDGLYFTMTKNKISTISSLHLFYISKKFHRGFLHCVADHPTGSVSSNGVQIMIKYPPEFINITIDNPYLNS--IYK---NKNYIFLDENVVQKINCITGEYYGSVNLKWKMD-DLSRGKDLRS-VEKIWQIQSNYKQQSSVSVTLGPTVDEIRLNCVMFFMDQFW-----LKSTVTIMKIKRPERLYIENYQSTFMLEENQVLKLKCLSFGGRPQPDLQWILNGETIVKSENQGLFKTNNSVDFELKVQRIHNTVILKCETRLEHYQTKTVTSQPIKLTVVFPPLKIKIEHNFPNYLLENEDVV-FNCVTTESNPPSGLIWKIKVCKYSNIE--------NCTEHILSDVKEYIFAGTQFHGYKTKSILKLSMNWEYHLAVLICESLFSYSDNSYPVIRSQIQLFISFKPNFINIGNFSQILLTLDSLNQSIIVKTVANPLPIRFKW------LFKRDSPSISTTDNKIFFQKVDESIKQEYQLIATNSLGDSVLIFNVDITYSPRIVGERFMKLPIHSKITELECLIESNPSPQISKINWLFF---SSYN--------------------YFDENFDEIPKKYSCNQGHWKNKI----MKLFVKCRLISPKIIASSLIIYNTSPEDVGTYICSASDNRSLSENKTIELITISIPNFISIPLFNKVAILLNTRSLAKAFMHSGLTSNITLHIELH---LNKSVLFPVKVNRINSGIYLV 3022
D E S A+IKQS CT ++I + IQCF +P P Y V + C++ NQ+GKVQW+A LLGYDR I GRP YR+VG KGEH L+I NV+++D G YECQVQP+G +HP LRR T L ++V P+ P+++++G + V +++ Q E TC+S +G+P+V F+W++N + +E L+ S S+L + K I C V+N AT + P ++L ++I TPP P+I K V+++I + C +P G PA SL W W+ ++N NYT I Y+ T +K +S+L + ++ K H L CVA +P S S V++ I + P + I++ + L+S I K N+N I+ + + I C T YY +KW + + D S VEKIW+ ++++ S++ + + D+ L+C Q W ++S V + PE+ I+ Y ++ N ++ C + GG P P+LQW + + I S ++ + N V C+ + ++ + S + L V+FPP+ I ++ + +EN ++ C ESNPP+ + WK+ C E C E +LS G +F G +S ++ +W+ HL + CE + V+R +IQ+ ++F P F I N++ + +Q++ ++ +ANP + W L +++ SIST+ K+ + Y L+ N G+S + ++ITY P I+G++F + I + ELEC ++SNP+ W YN + E +E PK YS + WK K F KC + S + S LIIY+ DVG Y C ++ KTIEL P +S+P F+K A L ++ S + +T + ++ + + + + R +G Y+
Sbjct: 39 DSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQDNENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQD-PERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDESFRRNSTIKIKINKQFDQSFLDC------QAWSGIKSVQSRVKLHITYSPEKPTIQGYNVGKPVKLNHNFEMTCSTIGGNPYPELQWFRDSKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLK-SLSGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTING-KFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQFEGIINYN--FYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYS--KAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVA 1053 HSP 2 Score: 52.7582 bits (125), Expect = 8.046e-127 Identity = 45/136 (33.09%), Postives = 69/136 (50.74%), Query Frame = 1
Query: 3001 QFRNIFSDFRIQLSDFGR--HRKNLFRAENKIGSSSLMLEAVSESIPDPPIVHEIKFLQPNSLFVKIIQTFDGGYPQSFQVFWKEANRNTTENKMWEVPGDYTK---KFIIIKI-SDTFLSKNYEIQVCSKNLVYG 3390
+FR F ++++ + R H + +AEN GS S +E S PD P+ ++ + + + IQ FDGG Q+FQV WKE + T+ + +V D TK FII + SDT Y I + S+N +G
Sbjct: 1045 RFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSP-TSLYRYEDVASDDTKITLSFIITGLKSDTA----YLISINSRNSKHG 1175 The following BLAST results are available for this feature:
BLAST of NPHS1-6 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of NPHS1-6 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of NPHS1-6 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of NPHS1-6 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of NPHS1-6 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of NPHS1-6 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of NPHS1-6 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of NPHS1-6 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of NPHS1-6 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of NPHS1-6 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of NPHS1-6 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of NPHS1-6 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of NPHS1-6 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of NPHS1-6 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30012656 ID=SMED30012656|Name=NPHS1-6|organism=Schmidtea mediterranea sexual|type=transcript|length=3921bpback to top protein sequence of SMED30012656-orf-1 >SMED30012656-orf-1 ID=SMED30012656-orf-1|Name=SMED30012656-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=223bp MLEAVSESIPDPPIVHEIKFLQPNSLFVKIIQTFDGGYPQSFQVFWKEANback to top protein sequence of SMED30012656-orf-2 >SMED30012656-orf-2 ID=SMED30012656-orf-2|Name=SMED30012656-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1030bp MLFLFYCWCLILEICENVSLNSNTTLFSINSRNFKTLNELARDLENSQEAback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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