Retrovirus-related Pol polyprotein from transposon
|
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Homology
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Human
Match: RTL1 (retrotransposon Gag like 1 [Source:HGNC Symbol;Acc:HGNC:14665]) HSP 1 Score: 62.3882 bits (150), Expect = 3.616e-9 Identity = 43/168 (25.60%), Postives = 82/168 (48.81%), Query Frame = 3
Query: 897 YMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKILWTKECEESFETLKKDYVKHQY*ILVEKIECLFYIQTPVSA 1400
++L+ ++ V+ Y ++LIY +Q+EH + + +V R + + + K ++ + FLG+ + GV+ N + I P S +R F+ YR F++ ++ IA PL + W E +E+FE LK+ + K + K + FY++T V+
Sbjct: 718 FILKDMLGFFVLSYGQEVLIYSMSQEEHLHHVRQVLVRFRHHNVYCSLDKSQFHRQTVEFLGFVVTPKGVKLNKNVMTIITGYPTPGSKLSLRNFIEFVFPYRHFVERFSIIAEPLVRQLLSS-YQFYWGVEEQEAFECLKRAFRKAPL-LHHPKPQNPFYLETGVTG 883
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Human
Match: RTL1 (retrotransposon Gag like 1 [Source:HGNC Symbol;Acc:HGNC:14665]) HSP 1 Score: 62.3882 bits (150), Expect = 3.616e-9 Identity = 43/168 (25.60%), Postives = 82/168 (48.81%), Query Frame = 3
Query: 897 YMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKILWTKECEESFETLKKDYVKHQY*ILVEKIECLFYIQTPVSA 1400
++L+ ++ V+ Y ++LIY +Q+EH + + +V R + + + K ++ + FLG+ + GV+ N + I P S +R F+ YR F++ ++ IA PL + W E +E+FE LK+ + K + K + FY++T V+
Sbjct: 718 FILKDMLGFFVLSYGQEVLIYSMSQEEHLHHVRQVLVRFRHHNVYCSLDKSQFHRQTVEFLGFVVTPKGVKLNKNVMTIITGYPTPGSKLSLRNFIEFVFPYRHFVERFSIIAEPLVRQLLSS-YQFYWGVEEQEAFECLKRAFRKAPL-LHHPKPQNPFYLETGVTG 883
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Zebrafish
Match: FO704673.1 (pep chromosome:GRCz11:12:14545475:14551077:-1 gene:ENSDARG00000112601.1 transcript:ENSDART00000188717.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:FO704673.1) HSP 1 Score: 112.849 bits (281), Expect = 6.652e-48 Identity = 53/144 (36.81%), Postives = 84/144 (58.33%), Query Frame = 3
Query: 900 MLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKILWTKECEESFETLKKDYV 1331
+ L+ K V+ Y+DDILIY N ++H + V R+Q+ L +KC + ++ FLGY I GV N+ K++ + +PK+ K+++ FLG N+YRRFI++Y+ I+APL + G K+ W E +SFE LK +
Sbjct: 643 VFRDLLNKCVIAYIDDILIYSPNLEQHIKDVRTVLTRLQENQLYAKLEKCEFHMSKTSFLGYIISHHGVEMNNTKVQAVTGWPLPKTVKELQRFLGFANFYRRFIRNYSLISAPLTSLLKGKPSKLKWNPETVKSFEKLKTSFT 786 HSP 2 Score: 76.6406 bits (187), Expect = 6.652e-48 Identity = 40/125 (32.00%), Postives = 67/125 (53.60%), Query Frame = 1
Query: 523 QKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLMH 897
Q +E I++ L+ IR S + I K+DG R C+DYRGLN IT K + +P L+ L ++ ++ +DL A + + + +TAF T G + + +P+GL+ +PA FQ ++
Sbjct: 517 QAMETYIEEALSSGYIRPSTSPAAAGFFFIEKKDGGLRPCIDYRGLNNITVKYRYPLPLVPPALEQLREARIYTKLDLRSAYNLIRIREGDEWKTAFLTTRGHYEYLVMPYGLANSPAVFQSFIN 641 HSP 3 Score: 43.5134 bits (101), Expect = 6.652e-48 Identity = 37/146 (25.34%), Postives = 55/146 (37.67%), Query Frame = 2
Query: 1304 FRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQMKEDINT*HXXXXXXXXXXXAR----------------------------------TDHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSR 1639
F +K APIL F++ DAS IGAVLSQ + H F K A TDHK L ++ + ++ ++P+ W + ++F + YR G ++ AD LSR
Sbjct: 778 FEKLKTSFTTAPILKHPNPELPFVVEVDASDYGIGAVLSQRHGNPGKLHPCAYFSRKLTAAERNYDVGNKELLSMKAALEEWRHWLEGAVHPFQIITDHKNLEYIKSARR-LNPRQARWSLFFTRFNFTVTYRPGTKNHKADALSR 922
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Zebrafish
Match: CR925755.2 (pep chromosome:GRCz11:17:42486740:42492668:-1 gene:ENSDARG00000116402.1 transcript:ENSDART00000183946.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR925755.2) HSP 1 Score: 102.449 bits (254), Expect = 3.485e-43 Identity = 50/145 (34.48%), Postives = 82/145 (56.55%), Query Frame = 3
Query: 900 MLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKILWTKECEESFETLKKDYVK 1334
+L +I + + VYLDDILI+ + QEH + V R+ + GL + +KC + + FLG+T+ G+R + KI+ + + P S K ++ FLG N+YRRFI+++ ++AAPL T + W+ E +F LK +V
Sbjct: 637 VLRDMIDQFIYVYLDDILIFSHSLQEHVQHVRRVLQRLLENGLYVKAEKCVFHAQSVQFLGHTVSVEGMRMDPEKIQAVVNWPTPDSRKALQRFLGFANFYRRFIRNFRQLAAPL-TNLTSSKTPFRWSNAAEAAFSKLKGCFVS 780 HSP 2 Score: 93.5893 bits (231), Expect = 3.485e-43 Identity = 47/125 (37.60%), Postives = 72/125 (57.60%), Query Frame = 1
Query: 523 QKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLMH 897
+ +E I D L +IR S + + K+DGS R C+DYRGLN+IT K ++ +P + + L + +F+ +DL A + V + P + +TAF+T G F + LPFGLS PA FQ L++
Sbjct: 511 EAMEKYISDSLAAKIIRPSSSPAGAGFFFVKKKDGSLRPCIDYRGLNSITVKNTYPLPLMSSAFERLQGANFFTKLDLRNAYHLVRIRPGDEWKTAFNTPRGHFEYCVLPFGLSNAPAVFQALVN 635
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Zebrafish
Match: BX546500.1 (pep chromosome:GRCz11:23:12926092:12931693:-1 gene:ENSDARG00000086495.3 transcript:ENSDART00000122176.3 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX546500.1) HSP 1 Score: 102.064 bits (253), Expect = 4.934e-43 Identity = 49/145 (33.79%), Postives = 83/145 (57.24%), Query Frame = 3
Query: 900 MLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKILWTKECEESFETLKKDYVK 1334
+L +I + + VYLDDILI+ + QEH + V R+ + GL + +KC + + FLG+ + G+R + KI+ + P S K ++ FLG N+YRRFI++++++AAPL + T+ + W+ E +F LK +V
Sbjct: 638 VLRDMIDQFIYVYLDDILIFSHSLQEHVQHVRRVLQRLLENGLYVKAEKCVFHAQSVQFLGHIVSVEGMRMDPEKIQAVVDWPTPDSRKALQRFLGFANFYRRFIRNFSQLAAPLTSLTSS-KMPFRWSSAAEAAFSKLKGCFVS 781 HSP 2 Score: 93.5893 bits (231), Expect = 4.934e-43 Identity = 47/125 (37.60%), Postives = 72/125 (57.60%), Query Frame = 1
Query: 523 QKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLMH 897
+ +E I D L +IR S + + K+DGS R C+DYRGLN+IT K ++ +P + + L + +F+ +DL A + V + P + +TAF+T G F + LPFGLS PA FQ L++
Sbjct: 512 EAMEKYISDSLAAKIIRPSSSPAGAGFFFVKKKDGSLRPCIDYRGLNSITVKNTYPLPLMSSAFERLQGANFFTKLDLRNAYHLVRIRPGDEWKTAFNTPRGHFEYCVLPFGLSNAPAVFQALVN 636
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Zebrafish
Match: BX511224.1 (pep chromosome:GRCz11:2:18017000:18022765:1 gene:ENSDARG00000113243.1 transcript:ENSDART00000186877.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX511224.1) HSP 1 Score: 101.679 bits (252), Expect = 2.131e-42 Identity = 49/145 (33.79%), Postives = 83/145 (57.24%), Query Frame = 3
Query: 900 MLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKILWTKECEESFETLKKDYVK 1334
+L +I + + VYLDDILI+ + QEH + V R+ + GL + +KC + + FLG+ + G+R + KI+ + + P S K ++ FLG N+YRRFI +++++AAPL + T+ + W+ E +F LK +V
Sbjct: 664 VLRDMIDQFIYVYLDDILIFSHSLQEHIQHVRRVLQRLLENGLYVKAEKCVFHAQSVQFLGHIVSVEGMRMDPEKIQAVVNWPTPDSRKALQRFLGFANFYRRFIHNFSQLAAPLTSLTSS-KTPFRWSSAAEAAFSKLKGCFVS 807 HSP 2 Score: 92.0485 bits (227), Expect = 2.131e-42 Identity = 47/125 (37.60%), Postives = 72/125 (57.60%), Query Frame = 1
Query: 523 QKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLMH 897
+ +E I D L +IR S + + K+DGS R C+DYRGLN+IT K ++ +P + + L + +F+ +DL A + V + P + +TAF+T G F + LPFGLS PA FQ L++
Sbjct: 538 EAMEKYISDSLAAKIIRPSSSPAGAGFFFVKKKDGSLRPCIDYRGLNSITVKNTYPLPLMSSAFERLQGANFFTKLDLRNAYHLVCIRPGDEWKTAFNTPRGHFEYCVLPFGLSNAPAVFQALVN 662
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Zebrafish
Match: BX511082.1 (pep chromosome:GRCz11:9:14291932:14297132:1 gene:ENSDARG00000113678.1 transcript:ENSDART00000183119.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX511082.1) HSP 1 Score: 101.293 bits (251), Expect = 3.963e-42 Identity = 47/145 (32.41%), Postives = 84/145 (57.93%), Query Frame = 3
Query: 900 MLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKILWTKECEESFETLKKDYVK 1334
+L ++ + + VYLDDILI+ + QEH + V R+ + GL + +KC + + FLG+ + G+R + K++ + P S K ++ FLG N+YRRFI++++++AAPL A T+ + W+ + +F+ LK +V
Sbjct: 664 VLRDMLDQFIYVYLDDILIFSHSLQEHVQHVRRVLQRLLENGLYVKAEKCVFHAQSVPFLGHIVSVEGMRMDPEKVQAVVDWPTPDSRKALQRFLGFANFYRRFIRNFSQLAAPLTALTS-LKTPFRWSNAAQVAFDRLKSCFVS 807 HSP 2 Score: 91.6633 bits (226), Expect = 3.963e-42 Identity = 47/125 (37.60%), Postives = 71/125 (56.80%), Query Frame = 1
Query: 523 QKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLMH 897
+ +E I D L +IR S + + K+DGS R C+DYRGLNAIT K ++ +P + + L + +F+ +DL A + V + + +TAF+T G F + LPFGLS PA FQ L++
Sbjct: 538 EAMEKYISDSLAAKIIRPSSSPAGAGFFFVKKKDGSLRPCIDYRGLNAITVKNTYPLPLMSSAFERLQGASFFTKLDLRNAYHLVRIREGDEWKTAFNTPRGHFEYCVLPFGLSNAPAVFQALVN 662
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Xenopus
Match: anxa6 (annexin A6 [Source:Xenbase;Acc:XB-GENE-989741]) HSP 1 Score: 115.161 bits (287), Expect = 7.179e-61 Identity = 63/173 (36.42%), Postives = 98/173 (56.65%), Query Frame = 1
Query: 391 LSKFGNIFMASKFDLGYTELVKHEIKTMGNPVMQHPRRQPMH-----LEQKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLM 894
L + +F A+ G T +H++ T Q P R P + + +++ I ++L VI S W S VV + K+DGSTR C+DYR LN +T +++ MP LLD L N+KY +++DL + +Q+ +AP Q+++AF T G + F +PFG+ PATFQRL+
Sbjct: 309 LRSYSPMFSANP---GRTHWAEHKVDTG----TQLPIRSPAYRVAEAVRPEMKSQIDEMLAFGVITPSHSPWASPVVLVPKKDGSTRFCVDYRRLNDVTTTDAYPMPRVDELLDRLGNAKYLTTLDLSRGYWQIPLAPSAQEKSAFLTPFGLYQFTVMPFGMRNAPATFQRLV 474 HSP 2 Score: 113.235 bits (282), Expect = 7.179e-61 Identity = 55/149 (36.91%), Postives = 88/149 (59.06%), Query Frame = 3
Query: 879 FSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKK-ILWTKECEESFETLKK 1322
F ++ +LEG+ YLDDI ++ + +EH L+ VF +IQ AGL + P+KCH+ E+ +LG+ + +R + K+E I +PK+ KQ+ FLG + YYR+FI +Y+ +A PL T+ + I+WT ECE + LK+
Sbjct: 470 FQRLVNRLLEGMQ-DFAQAYLDDIAVFSQTWEEHLQHLQRVFAQIQDAGLTLKPEKCHLAMAEVQYLGHRVGGGQLRPDPAKVEAICQWPIPKTQKQVLAFLGTSGYYRKFIPNYSTVAKPLTDLTSRQRSRTIVWTPECESAMNALKQ 617 HSP 3 Score: 48.9062 bits (115), Expect = 7.179e-61 Identity = 38/139 (27.34%), Postives = 65/139 (46.76%), Query Frame = 2
Query: 1313 VKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQMK---EDINT*HXXXXXXXXXXXARTDHKALVFLSTTKKAISPQFH-------------TWMANLS--------------EYDFELKYRKGEEHSNADGLSR 1639
+K+ L +P+L +R FIL TDAS +GAVLSQ+ E+ ++ + ++ T K + + + + P + +W+ +S +Y+F +++RKG+EH NADGLSR
Sbjct: 615 LKQALASSPVLAAPDFSRRFILQTDASNFGLGAVLSQVNTYGEEHPVAYLSRKLLPREAAYATIEKECLAIVWALQKLQPYLYGREFTVVTDHNPLSWLQRVSGDNGKLLRWSLLLQQYNFTIQHRKGKEHHNADGLSR 753
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Xenopus
Match: ENSXETT00000006041.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:6:37317867:37322724:1 gene:ENSXETG00000003070.1 transcript:ENSXETT00000006041.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 119.013 bits (297), Expect = 2.912e-53 Identity = 70/179 (39.11%), Postives = 106/179 (59.22%), Query Frame = 1
Query: 364 KTR*RKHSNLSKFGNIFMASKFDLGYTELVKHEIKTMGN-PVMQHPRR-QPMHLEQKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLM 894
K+R RKH L++ +F + D+G + KH+I+ + P + RR P LE + +++L +I++ +S + S +V + K++GS RMC+DYR LN T + +T P L+CLV SK+FS +DL YQ+ + PE +++TAF G F FNR+P GL PATFQRLM
Sbjct: 288 KSRLRKH--LNEHSALFSRNDLDMGCSTSTKHKIRLREDKPFRERSRRIAPGDLED-LRKHLEELKAAGIIKESRSPYASPIVVVRKKNGSIRMCVDYRTLNQRTIPDQYTTPRIEDALNCLVGSKWFSVLDLRSGYYQLPMHPEDKEKTAFICPLGFFEFNRMPQGLCGAPATFQRLM 463 HSP 2 Score: 111.694 bits (278), Expect = 2.912e-53 Identity = 54/166 (32.53%), Postives = 96/166 (57.83%), Query Frame = 3
Query: 891 DAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPL------YATTTGCEKKIL-----------------WTKECEESFETLK 1319
D ++LE V+VYLDD++++G+ +EH+ L +VF+R+++ GLK++P+KC + + ++G+ + G+ T+ KIE + S P++ ++R FLG YYRRF+K ++K+A PL G ++ IL WT+EC+++F+ LK
Sbjct: 469 DMHLLE------VLVYLDDLIVFGRTLEEHEQRLLKVFDRLEKEGLKLSPEKCQFCQPSVNYVGHVVSAEGIATDPSKIEAVSSWPKPRTITELRSFLGFCGYYRRFVKGFSKVAQPLNQLLQSNTEVEGSDRDILAKRLRGQGWTRESIEDFWTEECDKAFDQLK 628
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Xenopus
Match: alkbh5 (alkB homolog 5, RNA demethylase [Source:Xenbase;Acc:XB-GENE-987580]) HSP 1 Score: 122.865 bits (307), Expect = 4.657e-49 Identity = 68/175 (38.86%), Postives = 102/175 (58.29%), Query Frame = 1
Query: 436 GYTELVKHEIKTM-GNPVMQHPRRQPMHLEQKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLMHTC*KV--*YSKVWLSTSTIF 951
G TE++KH+I T G V P R P Q V IK +L +VI + S W+S +V + K DGS R C D+R LN +++ +++ MP L++ L ++Y +++DL + +QV + +++TAFST EGQ+ + RLPFGL PATFQR+M K Y+ +L IF
Sbjct: 219 GLTEMIKHDIITGPGVKVNVRPYRIPEARRQAVAGEIKRMLELDVIEESHSEWSSPIVLVPKPDGSIRFCNDFRKLNEVSKFDAYPMPRVDELIERLGQARYITTLDLTKGYWQVPLTEGAKEKTAFSTPEGQWQYKRLPFGLHGAPATFQRMMDRILKPHRGYASAYLDDVVIF 393 HSP 2 Score: 93.9745 bits (232), Expect = 4.657e-49 Identity = 45/112 (40.18%), Postives = 66/112 (58.93%), Query Frame = 3
Query: 933 VYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCE 1268
YLDD++I+ + + H ++ V + I +AGL NPKKC + E +LGY I V+ KIE I+ P S KQ+R FLG+ YYRRF+ ++A +A+PL T G +
Sbjct: 385 AYLDDVVIFSPDWESHLPRVQAVLDSIWKAGLTANPKKCAMGLEEARYLGYVIGRGVVKPQVSKIEGIQQWPRPNSKKQVRAFLGIVGYYRRFVPNFASLASPLTDLTKGSK 496
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Xenopus
Match: ENSXETT00000034712.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:9:33567219:33572531:-1 gene:ENSXETG00000011772.1 transcript:ENSXETT00000034712.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 97.8265 bits (242), Expect = 7.329e-44 Identity = 48/144 (33.33%), Postives = 82/144 (56.94%), Query Frame = 3
Query: 900 MLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKILWTKECEESFETLKKDYV 1331
+ L+ KSVVVYLDDILI+ ++ + H + ++E +R+++ L +KC ++ FLGY I G + K+ I+ +P+S K I+ F+G NYYR+FIKD++ AP+ + + W E+F++LK ++
Sbjct: 660 IFRDLLGKSVVVYLDDILIFSQDLETHRSQVKEALSRLRENFLFAKLEKCTFEVPKISFLGYIISSRGFEMDPAKVSAIQKWPLPQSTKAIQRFIGFANYYRQFIKDFSSRIAPILSLIRKGGRPNCWPPVALEAFQSLKDAFI 803 HSP 2 Score: 81.2629 bits (199), Expect = 7.329e-44 Identity = 46/143 (32.17%), Postives = 73/143 (51.05%), Query Frame = 1
Query: 481 PVMQHPRRQPMHLEQKVEVLIKDLLNDNV----IRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLMH 897
P PR + L +K+ +++N+ IR S + + K+DG R C+DYRGLN IT K + +P L D L +K FS +DL A + + + +TAF+T++G + + +PFGL PA FQ ++
Sbjct: 516 PGTMPPRGRTYPLSPAETAAMKEYISENLQRGFIRPSTSPAGAGFFFVEKKDGGLRPCIDYRGLNKITVKNRYPLPLISELFDQLKGAKIFSKLDLRGAYNLIRIREGDEWKTAFNTRDGHYEYLVMPFGLCNAPAVFQEFVN 658 HSP 3 Score: 40.817 bits (94), Expect = 7.329e-44 Identity = 38/146 (26.03%), Postives = 53/146 (36.30%), Query Frame = 2
Query: 1304 FRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQMKEDINT*HXXXXXXXXXXXAR----------------------------------TDHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSR 1639
F+++K A +L + F + DAS GA+LSQ H F K A TDHK L FL + K+ +P+ W S ++F L YR G ++ AD LSR
Sbjct: 795 FQSLKDAFISASVLRHPEPHLPFFIEVDASDVGAGAILSQRHSADGKLHPCAYFSKKFSSAEQNYDIGNRELLAVKLALEEWRHLLEGASHPVTIYTDHKNLEFLQSLKRQ-NPRQARWSLFFSRFNFVLTYRPGTKNRKADALSR 939
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Xenopus
Match: ENSXETT00000042189.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV460667.1:82848:87648:1 gene:ENSXETG00000017765.1 transcript:ENSXETT00000042189.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 97.8265 bits (242), Expect = 1.161e-43 Identity = 48/144 (33.33%), Postives = 82/144 (56.94%), Query Frame = 3
Query: 900 MLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKILWTKECEESFETLKKDYV 1331
+ L+ KSVVVYLDDILI+ ++ + H + ++E +R+++ L +KC ++ FLGY I G + K+ I+ +P+S K I+ F+G NYYR+FIKD++ AP+ + + W E+F++LK ++
Sbjct: 660 IFRDLLGKSVVVYLDDILIFSQDLETHRSQVKEALSRLRENFLFAKLEKCTFEVPKISFLGYIISSRGFEMDPAKVSAIQKWPLPQSTKAIQRFIGFANYYRQFIKDFSSHIAPILSLIRKGGRPNCWPPVALEAFQSLKDAFI 803 HSP 2 Score: 81.6481 bits (200), Expect = 1.161e-43 Identity = 46/143 (32.17%), Postives = 73/143 (51.05%), Query Frame = 1
Query: 481 PVMQHPRRQPMHLEQKVEVLIKDLLNDNV----IRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLMH 897
P PR + L +K+ +++N+ IR S + + K+DG R C+DYRGLN IT K + +P L D L +K FS +DL A + + + +TAF+T++G + + +PFGL PA FQ ++
Sbjct: 516 PGTMPPRGRTYPLSPAETAAMKEYISENLQRGFIRPSTSPAGAGFFFVEKKDGGLRPCIDYRGLNKITVKNRYPLPLISELFDQLKGAKIFSKLDLRGAYNLIRIREGDEWKTAFNTRDGHYEYLVMPFGLCNAPAVFQEFVN 658 HSP 3 Score: 39.6614 bits (91), Expect = 1.161e-43 Identity = 37/146 (25.34%), Postives = 53/146 (36.30%), Query Frame = 2
Query: 1304 FRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQMKEDINT*HXXXXXXXXXXXAR----------------------------------TDHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSR 1639
F+++K A +L + F + DAS GA+LSQ H F K A TDHK L FL + K+ +P+ W + ++F L YR G ++ AD LSR
Sbjct: 795 FQSLKDAFISASVLRHPEPHLPFFIEVDASDVGAGAILSQRHSADGKLHPCAYFSKKFSSAEQNYDIGNRELLAVKLALEEWRHLLEGASHPVTIYTDHKNLEFLQSLKRQ-NPRQARWSLFFTRFNFVLTYRPGTKNRKADALSR 939
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Mouse
Match: Rtl1 (retrotransposon Gaglike 1 [Source:MGI Symbol;Acc:MGI:2656842]) HSP 1 Score: 68.1662 bits (165), Expect = 4.251e-11 Identity = 42/170 (24.71%), Postives = 84/170 (49.41%), Query Frame = 3
Query: 897 YMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKILWTKECEESFETLKKDYVKHQY*ILVEKIECLFYIQTPVSAQL 1406
++L+ ++ V+ + ++L+Y +Q+EH + +V R + + + K ++ LG+ I GV+ N + I VP S + ++ + L YR F++++A IAAPL E W +E +E+ E+LK+ + K + K + FY++T ++
Sbjct: 979 FILKDILGLFVICHGREVLVYSMSQEEHSQHVRQVLVRFRYHNIYCSLDKTQFHRQTAEILGFNISPKGVKLNKNLMNLIVGCPVPGSRRCLQSVIDLVYPYRHFVENFAVIAAPLVRQLLSSE-PYYWGEEEQEALESLKRAFRKSPV-LYHPKPQNPFYLETDITGSF 1146
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Mouse
Match: Rtl1 (retrotransposon Gaglike 1 [Source:MGI Symbol;Acc:MGI:2656842]) HSP 1 Score: 68.1662 bits (165), Expect = 4.251e-11 Identity = 42/170 (24.71%), Postives = 84/170 (49.41%), Query Frame = 3
Query: 897 YMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKILWTKECEESFETLKKDYVKHQY*ILVEKIECLFYIQTPVSAQL 1406
++L+ ++ V+ + ++L+Y +Q+EH + +V R + + + K ++ LG+ I GV+ N + I VP S + ++ + L YR F++++A IAAPL E W +E +E+ E+LK+ + K + K + FY++T ++
Sbjct: 979 FILKDILGLFVICHGREVLVYSMSQEEHSQHVRQVLVRFRYHNIYCSLDKTQFHRQTAEILGFNISPKGVKLNKNLMNLIVGCPVPGSRRCLQSVIDLVYPYRHFVENFAVIAAPLVRQLLSSE-PYYWGEEEQEALESLKRAFRKSPV-LYHPKPQNPFYLETDITGSF 1146
BLAST of Retrovirus-related Pol polyprotein from transposon vs. UniProt/SwissProt
Match: sp|P20825|POL2_DROME (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1) HSP 1 Score: 103.99 bits (258), Expect = 6.469e-55 Identity = 61/151 (40.40%), Postives = 88/151 (58.28%), Query Frame = 3
Query: 879 FSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKI--LWTKECE--ESFETLK 1319
F + +L L+ K +VYLDDI+I+ + EH N ++ VF ++ A LK+ KC K E FLG+ + G++ N K++ I S +P K+IR FLGLT YYR+FI +YA IA P+ T C KK + T++ E E+FE LK
Sbjct: 344 FQRCMNNILRPLLNKHCLVYLDDIIIFSTSLTEHLNSIQLVFTKLADANLKLQLDKCEFLKKEANFLGHIVTPDGIKPNPIKVKAIVSYPIPTKDKEIRAFLGLTGYYRKFIPNYADIAKPM----TSCLKKRTKIDTQKLEYIEAFEKLK 490 HSP 2 Score: 102.834 bits (255), Expect = 6.469e-55 Identity = 63/177 (35.59%), Postives = 100/177 (56.50%), Query Frame = 1
Query: 391 LSKFGNIFMASKFDLGYTELVKHEIKTMGN-PVM--QHPRRQPMHLEQKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGST-----RMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLMH 897
L+KF N+ L +T +KH + T N P+ Q+P Q +E VE ++++LN +IR+ S +NS + K+ ++ R+ +DYR LN IT + + +P +L L +YF++IDL + +Q+E+ E +TAFSTK G + + R+PFGL PATFQR M+
Sbjct: 175 LNKFRNLEYKEGEKLTFTNTIKHVLNTTHNSPIYSKQYPLAQTHEIE--VENQVQEMLNQGLIRESNSPYNSPTWVVPKKPDASGANKYRVVIDYRKLNEITIPDRYPIPNMDEILGKLGKCQYFTTIDLAKGFHQIEMDEESISKTAFSTKSGHYEYLRMPFGLRNAPATFQRCMN 349 HSP 3 Score: 52.373 bits (124), Expect = 6.469e-55 Identity = 43/165 (26.06%), Postives = 66/165 (40.00%), Query Frame = 2
Query: 1304 FRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQMKEDINT*HXXXXXXXXXXXA--------------------------RTDHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSRINGDVCAQCQTRHKDTKQEKPRVRHVC 1720
F +K + PIL + F+L TDAS ++GAVLSQ I+ N A +DH+ L +L K+ + W LSEY F++ Y KG+E+S AD LSRI + + +++ + H+
Sbjct: 486 FEKLKALIIRDPILQLPDFEKKFVLTTDASNLALGAVLSQNGHPISFISRTLNDHELNYSAIEKELLAIVWATKTFRHYLLGRQFLIASDHQPLRWLHNLKEP-GAKLERWRVRLSEYQFKIDYIKGKENSVADALSRIKIEENHHSEATQHSAEEDNSNLIHLT 649
BLAST of Retrovirus-related Pol polyprotein from transposon vs. UniProt/SwissProt
Match: sp|Q8I7P9|POL5_DROME (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1) HSP 1 Score: 98.2117 bits (243), Expect = 9.343e-50 Identity = 58/177 (32.77%), Postives = 95/177 (53.67%), Query Frame = 1
Query: 382 HSNLSKFGNIFMASKFDLGYTELVKHEIKT-MGNPVMQHPRRQPMHLEQKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKE-----DGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLM 894
+S L +F IF + VK EI+T +P+ P+++ +VE I +LL D +IR S +NS + + K+ + RM +D++ LN +T +++ +P L L N+KYF+++DL +Q+ + +TAFST G++ F RLPFGL PA FQR++
Sbjct: 89 NSLLGEFPRIFEPPLSGMSVETAVKAEIRTNTQDPIYAKSYPYPVNMRGEVERQIDELLQDGIIRPSNSPYNSPIWIVPKKPKPNGEKQYRMVVDFKRLNTVTIPDTYPIPDINATLASLGNAKYFTTLDLTSGFHQIHMKESDIPKTAFSTLNGKYEFLRLPFGLKNAPAIFQRMI 265 HSP 2 Score: 95.9005 bits (237), Expect = 9.343e-50 Identity = 46/128 (35.94%), Postives = 76/128 (59.38%), Query Frame = 3
Query: 879 FSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTG 1262
F ++ +L I K VY+DDI+++ ++ H L V + +A L++N +K H T++ FLGY + G++ + +K+ I P S K+++ FLG+T+YYR+FI+DYAK+A PL T G
Sbjct: 261 FQRMIDDILREHIGKVCYVYIDDIIVFSEDYDTHWKNLRLVLASLSKANLQVNLEKSHFLDTQVEFLGYIVTADGIKADPKKVRAISEMPPPTSVKELKRFLGMTSYYRKFIQDYAKVAKPLTNLTRG 388 HSP 3 Score: 47.7506 bits (112), Expect = 9.343e-50 Identity = 43/152 (28.29%), Postives = 65/152 (42.76%), Query Frame = 2
Query: 1280 MDKGMRGKFRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQ-----------MKEDINT*HXXXXXXXXXXXA--------------------RTDHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSRI 1642
+D+ F ++K LC + IL F + F L TDAS +IGAVLSQ + +N K+++A TDH+ L F + + + + W A + EY+ EL Y+ G+ + AD LSRI
Sbjct: 405 LDETALQSFNDLKSILCSSEILAFPCFTKPFHLTTDASNWAIGAVLSQDDQGRDRPIAYISRSLNKTEENYATIEKEMLAIIWSLDNLRAYLYGAGTIKVYTDHQPLTF-ALGNRNFNAKLKRWKARIEEYNCELIYKPGKSNVVADALSRI 555
BLAST of Retrovirus-related Pol polyprotein from transposon vs. UniProt/SwissProt
Match: sp|P04323|POL3_DROME (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1) HSP 1 Score: 112.464 bits (280), Expect = 1.819e-47 Identity = 67/174 (38.51%), Postives = 94/174 (54.02%), Query Frame = 1
Query: 391 LSKFGNIFMASKFDLGYTELVKHEIKTMGNPVMQHPRRQPMHLEQKVEVLIKDLLNDNVIRKCKSSWNSRV-VKINKEDGST----RMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLMH 897
L K+ +I L +T KH I T N + P EQ+VE I+D+LN +IR S +NS + V K+D S R+ +DYR LN IT + +P +L L YF++IDL + +Q+E+ PE +TAFSTK G + + R+PFGL PATFQR M+
Sbjct: 177 LQKYHDIQYHEGDKLTFTNQTKHTINTKHNLPLYSKYSYPQAYEQEVESQIQDMLNQGIIRTSNSPYNSPIWVVPKKQDASGKQKFRIVIDYRKLNEITVGDRHPIPNMDEILGKLGRCNYFTTIDLAKGFHQIEMDPESVSKTAFSTKHGHYEYLRMPFGLKNAPATFQRCMN 350 HSP 2 Score: 100.908 bits (250), Expect = 1.819e-47 Identity = 55/144 (38.19%), Postives = 82/144 (56.94%), Query Frame = 3
Query: 900 MLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKIL----WTKECEESFETLK 1319
+L L+ K +VYLDDI+++ + EH L VF ++ +A LK+ KC K E FLG+ + G++ N KIE I+ +P K+I+ FLGLT YYR+FI ++A IA P+ T C KK + E + +F+ LK
Sbjct: 352 ILRPLLNKHCLVYLDDIIVFSTSLDEHLQSLGLVFEKLAKANLKLQLDKCEFLKQETTFLGHVLTPDGIKPNPEKIEAIQKYPIPTKPKEIKAFLGLTGYYRKFIPNFADIAKPM----TKCLKKNMKIDTTNPEYDSAFKKLK 491 HSP 3 Score: 55.4546 bits (132), Expect = 1.882e-6 Identity = 59/199 (29.65%), Postives = 83/199 (41.71%), Query Frame = 2
Query: 1274 DFMDKGMRGKFRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQ------------MKEDINT*HXXXXXXXXXXXART--------------DHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSRIN-GDVCAQCQTRHKDTKQ--------EKPR---VRHVCFAKNGDDGKNTR 1756
D + F+ +K + E PIL + F L TDAS ++GAVLSQ + +IN I K +T DH+ L +L K S + W LSE+DF++KY KG+E+ AD LSRI + QT+H + E+P R V F+K D K T+
Sbjct: 477 DTTNPEYDSAFKKLKYLISEDPILKVPDFTKKFTLTTDASDVALGAVLSQDGHPLSYISRTLNEHEINYSTIEKELLAIVWATKTFRHYLLGRHFEISSDHQPLSWLYRMKDPNS-KLTRWRVKLSEFDFDIKYIKGKENCVADALSRIKLEETYLSEQTQHSAEEDNSDLIFITERPLNTFNRQVIFSKGPPDIKVTK 674
BLAST of Retrovirus-related Pol polyprotein from transposon vs. UniProt/SwissProt
Match: sp|P10394|POL4_DROME (Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaster OX=7227 GN=POL PE=4 SV=1) HSP 1 Score: 92.4337 bits (228), Expect = 2.135e-38 Identity = 52/174 (29.89%), Postives = 90/174 (51.72%), Query Frame = 1
Query: 394 SKFGNIFMASKFDLGYTELVKHEIKTMGN-PVMQHPRRQPMHLEQKVEVLIKDLLNDNVIRKCKSSWNSRVVKI------NKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLM 894
S++ +IF + L K +++ + PV R P ++++ ++ L+ D ++ S +NS ++ + N + R+ +DYR +N + F +P +LD L +KYFS +DL +Q+E+ +D T+FST G + F RLPFGL I P +FQR+M
Sbjct: 284 SEYIDIFALESEPITVNNLYKQQLRLKDDEPVYTKNYRSPHSQVEEIQAQVQKLIKDKIVEPSVSQYNSPLLLVPKKSSPNSDKKKWRLVIDYRQINKKLLADKFPLPRIDDILDQLGRAKYFSCLDLMSGFHQIELDEGSRDITSFSTSNGSYRFTRLPFGLKIAPNSFQRMM 457 HSP 2 Score: 90.5077 bits (223), Expect = 2.135e-38 Identity = 46/153 (30.07%), Postives = 84/153 (54.90%), Query Frame = 3
Query: 879 FSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKIL--WTKECEESFETLKKDYV 1331
F ++ G+ +Y+DD+++ G +++ L EVF + ++ LK++P+KC + E+ FLG+ + G+ + +K + I++ VP A R F+ NYYRRFIK++A + + T C+K + WT EC+++F LK +
Sbjct: 453 FQRMMTIAFSGIEPSQAFLYMDDLIVIGCSEKHMLKNLTEVFGKCREYNLKLHPEKCSFFMHEVTFLGHKCTDKGILPDDKKYDVIQNYPVPHDADSARRFVAFCNYYRRFIKNFADYSRHI---TRLCKKNVPFEWTDECQKAFIHLKSQLI 602
BLAST of Retrovirus-related Pol polyprotein from transposon vs. UniProt/SwissProt
Match: sp|P10401|POLY_DROME (Retrovirus-related Pol polyprotein from transposon gypsy OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1) HSP 1 Score: 81.2629 bits (199), Expect = 5.754e-36 Identity = 50/168 (29.76%), Postives = 88/168 (52.38%), Query Frame = 1
Query: 412 FMASKFDLGYTELVKHEIKTMGN-PVMQHPRRQPMHLEQKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKE------DGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLM 894
F + L + V I+T+ N PV M + V +K LL D +IR +S +NS ++K+ + + R+ +D+R LN T + + MP+ +L L +K+F+++DL+ +Q+ +A +++T+FS G++ F RLPFGL + FQR +
Sbjct: 157 FSTTNEALPFNTAVTATIRTVDNEPVYSRAYPTLMGVSDFVNNEVKQLLKDGIIRPSRSPYNSPTWVVDKKGTDAFGNPNKRLVIDFRKLNEKTIPDRYPMPSIPMILANLGKAKFFTTLDLKSGYHQIYLAEHDREKTSFSVNGGKYEFCRLPFGLRNASSIFQRAL 324 HSP 2 Score: 79.337 bits (194), Expect = 5.754e-36 Identity = 37/125 (29.60%), Postives = 72/125 (57.60%), Query Frame = 3
Query: 870 TGYFSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPL 1244
+ F + +L I K VY+DD++I+ +N+ +H ++ V + A ++++ +K +K + +LG+ + + G +++ K++ I+ P ++R FLGL +YYR FIKD+A IA P+
Sbjct: 317 SSIFQRALDDVLREQIGKICYVYVDDVIIFSENESDHVRHIDTVLKCLIDANMRVSQEKTRFFKESVEYLGFIVSKDGTKSDPEKVKAIQEYPEPDCVYKVRSFLGLASYYRVFIKDFAAIARPI 441 HSP 3 Score: 34.6538 bits (78), Expect = 5.754e-36 Identity = 35/149 (23.49%), Postives = 58/149 (38.93%), Query Frame = 2
Query: 1283 DKGMRGKFRNVKKRLC-EAPILNFSRENRMFILYTDASFCSIGAVLSQMKEDINT*HXXXXXXXXXXXAR---------------------------TDHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSRIN 1645
++ R F+ ++ L E IL + + F L TDAS IGAVLSQ I + TDH+ L F + + + + W + + +++ ++ Y+ G+E+ AD LSR N
Sbjct: 465 NETQRNAFQRLRNILASEDVILKYPDFKKPFDLTTDASASGIGAVLSQEGRPITMISRTLKQPEQNYATNERELLAIVWALGKLQNFLYGSREINIFTDHQPLTF-AVADRNTNAKIKRWKSYIDQHNAKVFYKPGKENFVADALSRQN 612
BLAST of Retrovirus-related Pol polyprotein from transposon vs. TrEMBL
Match: A0A0V1D0N4 (Transposon Ty3-G Gag-Pol polyprotein OS=Trichinella britovi OX=45882 GN=TY3B-G PE=4 SV=1) HSP 1 Score: 145.206 bits (365), Expect = 1.885e-79 Identity = 77/180 (42.78%), Postives = 107/180 (59.44%), Query Frame = 1
Query: 358 SGKTR*RKHSNLSKFGNIFMASKFDLGYTELVKHEIKTMG-NPVMQHPRRQPMHLEQKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLM 894
S + R S + ++G + DLG T LV+H I+T G PV PRR P + V+ LI+++L +I W+S VV + K+DGS R C+DYR LNA+T ++ +P LD L +K+FS++DL +QVEVA E +++TAFST G F F +PFGL PATFQRLM
Sbjct: 1889 SAQNRRTLRSLIRRYGKAISCGEGDLGRTSLVQHRIETGGAQPVKLPPRRLPQAQRETVDRLIREMLQAGIIEPASGPWSSPVVLVRKKDGSPRFCVDYRRLNAVTRVDAQPIPRIDDTLDALAGAKWFSTLDLASGYWQVEVAEEDREKTAFSTPLGLFQFRVMPFGLCNAPATFQRLM 2068 HSP 2 Score: 125.561 bits (314), Expect = 1.885e-79 Identity = 58/151 (38.41%), Postives = 95/151 (62.91%), Query Frame = 3
Query: 879 FSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKILWTKECEESFETLKKDYV 1331
F ++ L GL +K+ +VYLDDI+++ K ++EH LE V +R+Q GLKI P+KC + + + +LG+ + + G+ T+ K ++ P+ +++R FLGL +YYRRF++++A +A PL+A T E K W + EE+F LKK V
Sbjct: 2064 FQRLMEKALRGLTWKTCLVYLDDIIVFRKTEEEHLERLEGVLSRLQSVGLKIKPEKCQLMRQSVHYLGHVVTQQGIGTDPEKTAAVQKWPTPRCVREVRQFLGLASYYRRFVRNFAGVANPLHALTKKGE-KWHWGPKEEEAFTLLKKALV 2213 HSP 3 Score: 60.077 bits (144), Expect = 1.885e-79 Identity = 50/152 (32.89%), Postives = 70/152 (46.05%), Query Frame = 2
Query: 1304 FRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQM---------------------------KEDIN----T*HXXXXXXXXXXXARTDHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSRINGDVCAQC 1666
F +KK L PIL +R F+L DAS ++GAVLSQ +E + T H + ++ IARTDH +L +L ++ Q W+ L+E+DFE+ +R G +H NAD LSR VC QC
Sbjct: 2205 FTLLKKALVSPPILGHPDFDRTFLLDVDASEDAVGAVLSQQGGQDPPSVIAYASRSLSRAERGYCATRREMLALVWATQHFRPYLYGRRFIARTDHNSLRWLRNFREP-EGQVARWLERLAEFDFEVVHRAGRKHQNADALSR---RVCKQC 2352 HSP 4 Score: 38.5058 bits (88), Expect = 1.885e-79 Identity = 29/141 (20.57%), Postives = 60/141 (42.55%), Query Frame = 3
Query: 1728 KTETMERIQEEQHKDCKFQKVSEFLQDNTIQKKDLPK-----KFLFKQIDYIRYEEGLLIMERDGSH------LVLVPENYVEELVKQIH--VELCHVGIKKLSHYISNIFFWPSIQDSIPECLRKCVECAKRKFDQKRTK 2111
K + I++ Q D + Q++ E+ T + P+ + L+ Q D I EG + + + L ++P + E++ +H H+G+ K + ++WP ++ + + R C CA R K+ +
Sbjct: 2367 KLDAANPIKQWQESDKELQQIREWSTQRTWPRA-APEGSRLLRSLWAQRDRIVVREGTICRKWETPDTGETRLLQVIPRQRIPEILAAVHNGQSGAHLGVAKTLAKVRQRYYWPQQREDVEDWCRACETCAARAVPTKKPQ 2506 HSP 5 Score: 21.1718 bits (43), Expect = 1.885e-79 Identity = 9/31 (29.03%), Postives = 17/31 (54.84%), Query Frame = 2
Query: 2183 RVGKKYVVVIIDRFNKLVSLTATSKQDDQTI 2275
R G +Y++V+ D F+K A + +T+
Sbjct: 2533 RNGNRYILVVCDYFSKWPEAFALPNAEARTV 2563
BLAST of Retrovirus-related Pol polyprotein from transposon vs. TrEMBL
Match: A0A0V1L9H7 (Transposon Ty3-I Gag-Pol polyprotein (Fragment) OS=Trichinella nativa OX=6335 GN=TY3B-I PE=4 SV=1) HSP 1 Score: 146.747 bits (369), Expect = 1.207e-77 Identity = 75/166 (45.18%), Postives = 102/166 (61.45%), Query Frame = 1
Query: 400 FGNIFMASKFDLGYTELVKHEIKTMG-NPVMQHPRRQPMHLEQKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLM 894
+G + DLG T LV+H I+T G PV PRR P + V+ LI+++L+ VI W+S VV + K+DGS R C+DYR LNA+T ++ +P LD L +K+FS++DL +QVEVA E +++TAFST G F F +PFGL PATFQRLM
Sbjct: 2048 YGKAISCGEGDLGRTSLVQHRIETGGAQPVKLPPRRLPQAQRETVDRLIREMLHAGVIEPASGPWSSPVVLVRKKDGSPRFCVDYRRLNAVTRVDAQPIPRIDDTLDALAGAKWFSTLDLASGYWQVEVAEEDREKTAFSTPLGLFQFRVMPFGLCNAPATFQRLM 2213 HSP 2 Score: 119.783 bits (299), Expect = 1.207e-77 Identity = 50/131 (38.17%), Postives = 86/131 (65.65%), Query Frame = 3
Query: 879 FSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEK 1271
F ++ L GL +K+ +VYLDD++++GK ++EH LE V +R++ GLKI P+KC + + + +LG+ + + GV T+ K ++ P+ +++R FLGL +YYRRF++++A +A PL+A T EK
Sbjct: 2209 FQRLMEKALRGLTWKTCLVYLDDVIVFGKTEEEHLERLEGVLSRLRSVGLKIKPEKCQLMRQSVRYLGHIVTQHGVSTDPEKTAAVQEWPTPRCVREVRQFLGLASYYRRFVRNFAGVANPLHALTKKGEK 2339 HSP 3 Score: 52.373 bits (124), Expect = 1.207e-77 Identity = 42/133 (31.58%), Postives = 59/133 (44.36%), Query Frame = 2
Query: 1361 NRMFILYTDASFCSIGAVLSQMKED-------------------------------INT*HXXXXXXXXXXXARTDHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSRINGDVCAQC 1666
+R F++ DAS +IGAVLSQ E T H + +K ARTDH +L +L ++ Q W+ L+E+DFE+ +R G++H NAD LSR C QC
Sbjct: 2357 DRTFLVDVDASEDAIGAVLSQQGEQGPPGVVAYASRSLSRAERSYCATRKEMLALVWATHHFRPYLYGRKFTARTDHNSLKWLRNFREP-EGQVARWLEKLAEFDFEVVHRPGKKHQNADALSR---RACRQC 2485 HSP 4 Score: 45.4394 bits (106), Expect = 1.207e-77 Identity = 34/142 (23.94%), Postives = 63/142 (44.37%), Query Frame = 3
Query: 1722 LQKTETMERIQEEQHKDCKFQKVSEFLQDNT----IQKKDLPKKFLFKQIDYIRYEEGLLIME------RDGSHLVLVPENYVEELVKQIHVELC--HVGIKKLSHYISNIFFWPSIQDSIPECLRKCVECAKRKFDQKRTK 2111
L +ER Q+E D + +V E+++ T + L K L+ Q D I +EG + D L ++P + E++K IH + H+G+ K + ++WP ++ + + R C CA R ++ +
Sbjct: 2501 LDSASPVERWQKE---DAECMQVREWIEKETWPQLAPEGSLWMKSLWSQRDRIVLQEGTICRTWEIPDTGDSRLLPVIPRRNIPEILKTIHNQPTGGHLGVAKTLAKVRQRYYWPQQREDVEDWCRACQTCAARAIPTRKLQ 2639 HSP 5 Score: 148.673 bits (374), Expect = 6.255e-70 Identity = 76/169 (44.97%), Postives = 104/169 (61.54%), Query Frame = 1
Query: 391 LSKFGNIFMASKFDLGYTELVKHEIKTMG-NPVMQHPRRQPMHLEQKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLM 894
L ++G + DLG T LV+H I+T G PV PRR P + V+ LI+++L+ VI W+S VV + K+DGS R C+DYR LNA+T ++ +P LD L +K+FS++DL +QVEVA E +++TAFST G F F +PFGL PATFQRLM
Sbjct: 274 LRRYGKAISCGEGDLGRTSLVQHRIETGGAQPVKLPPRRLPQAQRETVDRLIREMLHAGVIEPASGPWSSPVVLVRKKDGSPRFCVDYRRLNAVTRVDAQPIPRIDDTLDALAGAKWFSTLDLASGYWQVEVAEEDREKTAFSTPLGLFQFRVMPFGLCNAPATFQRLM 442 HSP 6 Score: 91.6633 bits (226), Expect = 6.255e-70 Identity = 43/131 (32.82%), Postives = 74/131 (56.49%), Query Frame = 3
Query: 879 FSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEK 1271
F ++ L GL +K+ +VYLDD++++GK ++EH LE V +R++ GLKI P+KC + + + +LG+ + + GV T+ K ++ P RRF++++A +A PL+A T EK
Sbjct: 438 FQRLMEKALRGLTWKTCLVYLDDVIVFGKTEEEHLERLEGVLSRLRSVGLKIKPEKCQLMRQSVRYLGHIVTQHGVSTDPEKTTAVQEWPTP----------------RRFVRNFAGVANPLHALTKKGEK 552 HSP 7 Score: 52.373 bits (124), Expect = 6.255e-70 Identity = 42/133 (31.58%), Postives = 59/133 (44.36%), Query Frame = 2
Query: 1361 NRMFILYTDASFCSIGAVLSQMKED-------------------------------INT*HXXXXXXXXXXXARTDHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSRINGDVCAQC 1666
+R F++ DAS +IGAVLSQ E T H + +K ARTDH +L +L ++ Q W+ L+E+DFE+ +R G++H NAD LSR C QC
Sbjct: 570 DRTFLVDVDASEDAIGAVLSQQGEQGPPGVVAYASRSLSRAERSYCATRKEMLALVWATHHFRPYLYGRKFTARTDHNSLKWLRNFREP-EGQVARWLEKLAEFDFEVVHRPGKKHQNADALSR---RACRQC 698 HSP 8 Score: 45.4394 bits (106), Expect = 6.255e-70 Identity = 34/142 (23.94%), Postives = 63/142 (44.37%), Query Frame = 3
Query: 1722 LQKTETMERIQEEQHKDCKFQKVSEFLQDNT----IQKKDLPKKFLFKQIDYIRYEEGLLIME------RDGSHLVLVPENYVEELVKQIHVELC--HVGIKKLSHYISNIFFWPSIQDSIPECLRKCVECAKRKFDQKRTK 2111
L +ER Q+E D + +V E+++ T + L K L+ Q D I +EG + D L ++P + E++K IH + H+G+ K + ++WP ++ + + R C CA R ++ +
Sbjct: 714 LDSASPVERWQKE---DAECMQVREWIEKETWPQLAPEGSLWMKSLWSQRDRIVLQEGTICRTWEIPDTGDSRLLPVIPRRNIPEILKTIHNQPTGGHLGVAKTLAKVRQRYYWPQQREDVEDWCRACQTCAARAIPTRKLQ 852
BLAST of Retrovirus-related Pol polyprotein from transposon vs. TrEMBL
Match: A0A5J4NQX1 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0008956 PE=4 SV=1) HSP 1 Score: 145.591 bits (366), Expect = 3.483e-75 Identity = 73/169 (43.20%), Postives = 103/169 (60.95%), Query Frame = 1
Query: 391 LSKFGNIFMASKFDLGYTELVKHEIKTM-GNPVMQHPRRQPMHLEQKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLM 894
+ K IF G T V+H I T +P+ Q RR P+HL+ +VE L+ D+L +IR S W S VV + K+DGS R+C+DYR LNA+T+K+SF +P +D L +++FS++DL +QVEV P + +TAF G + F +PFGL+ PATFQRLM
Sbjct: 451 IEKHRAIFWWEGQPPGRTHFVRHRIDTGEAHPIRQAARRLPVHLQGQVEQLLTDMLEKGIIRPSTSPWASPVVLVKKKDGSLRLCVDYRQLNAVTKKDSFPLPRIDDTIDALSGTEWFSTLDLASGYWQVEVEPSDRLKTAFVVPSGLYEFETMPFGLTNAPATFQRLM 619 HSP 2 Score: 125.946 bits (315), Expect = 3.483e-75 Identity = 58/147 (39.46%), Postives = 95/147 (64.63%), Query Frame = 3
Query: 879 FSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKILWTKECEESFETLK 1319
F ++ +L LI K +VYLDD++++G+ EH + L V +R+ +AGLK+NP KC +TE+ +LG+ I +SGV + K+ ++K P+S ++IR FLGL +YYRRF+ ++ IA PL T + W+++C++SF+ LK
Sbjct: 615 FQRLMQQVLGDLIPKQCLVYLDDVIVHGRTVDEHLHNLSNVLSRLAEAGLKLNPAKCSFMRTEVKYLGHVISQSGVSCDPEKVRKVKEWPTPESVEEIRSFLGLASYYRRFVPRFSHIAKPLTLLTEK-GRVFEWSEDCQQSFDRLK 760 HSP 3 Score: 63.5438 bits (153), Expect = 3.483e-75 Identity = 56/163 (34.36%), Postives = 72/163 (44.17%), Query Frame = 2
Query: 1304 FRNVKKRLCEAPIL---NFSRENRMFILYTDASFCSIGAVLSQM----KEDINT---------------------------*HXXXXXXXXXXXARTDHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSRI----NGDVCAQCQTRH 1678
F +K LC APIL + + +MFIL TDAS +IGAVLSQ+ KE + H ++ + RTDH +L +L K Q W L EY+FE K+R G H NAD LSR +GD C C TR+
Sbjct: 756 FDRLKDALCTAPILALPDTRADAKMFILDTDASDVAIGAVLSQLDNNDKEHVIAYGNRNLNRSERNYCTTRKEMLALVHFIKHFRHYLLGRRFLVRTDHSSLQWLQNFKDP-EGQVARWQEFLQEYNFECKHRPGLRHRNADALSRRPIRDHGD-CPSC-TRY 915
BLAST of Retrovirus-related Pol polyprotein from transposon vs. TrEMBL
Match: A0A3B3HBB2 (Uncharacterized protein OS=Oryzias latipes OX=8090 PE=4 SV=1) HSP 1 Score: 135.961 bits (341), Expect = 1.443e-74 Identity = 69/178 (38.76%), Postives = 105/178 (58.99%), Query Frame = 1
Query: 364 KTR*RKHSNLSKFGNIFMASKFDLGYTELVKHEI-KTMGNPVMQHPRRQPMHLEQKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLM 894
++R R L + ++F A DLG T +V+H+I T G+ V Q PRR ++ + ++ L + R SSW + +V + K+D S R+C+DYR LN T K+++ +P + LD L +KYFS++DL +QVE+ P + AF T++G F +N +PFGL PATFQRLM
Sbjct: 792 ESRYRLAGLLRSYSDVFSAGSTDLGRTNVVQHDILTTPGSAVKQQPRRMSREKQEAADQQVQQSLEAGLARHSNSSWAAPIVMVKKKDQSPRLCVDYRPLNDRTIKDAYPLPRIQDTLDTLSTAKYFSTLDLTSGYWQVEMTPRARKAAAFCTRKGLFEWNVMPFGLCNAPATFQRLM 969 HSP 2 Score: 132.494 bits (332), Expect = 1.443e-74 Identity = 57/148 (38.51%), Postives = 99/148 (66.89%), Query Frame = 3
Query: 879 FSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKILWTKECEESFETLKK 1322
F ++ +L GL +++ +VYLDDI++ G N + LE+VF R++QA LK+ P KC +++ ++ +LG+ + G+ T+ +KI++++ +PK+ ++R F+GL +YYRRF+ D+A IA PL+ T + WT EC+E+FE LK+
Sbjct: 965 FQRLMDRVLAGLQWEACLVYLDDIIVLGCNSNQMMERLEQVFTRLRQANLKLKPSKCRLFQEQVAYLGHVVSAQGISTDPQKIQKVQDWPIPKNISEVRQFVGLASYYRRFVADFATIARPLHELTKK-YARFDWTTECQEAFEELKE 1111 HSP 3 Score: 64.6994 bits (156), Expect = 1.443e-74 Identity = 45/142 (31.69%), Postives = 68/142 (47.89%), Query Frame = 2
Query: 1304 FRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQMK----------------EDIN--------------T*HXXXXXXXXXXXARTDHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSR 1639
F +K+RL APIL + ++ +L TDAS +GAVLSQ++ + N T H + + + RTDH +L +L+ K+ Q W+ L+EYDF++ +R G+ H NAD LSR
Sbjct: 1106 FEELKERLTSAPILGYPLDSGELLLDTDASDWGVGAVLSQVQGGEERVLAYGSRRLTTTEQNYCTTRRELLAVVEFTSHFRQYLLGRSFVLRTDHSSLRWLTRLKEP-EGQLARWLEKLAEYDFQVLHRPGKVHQNADALSR 1246
BLAST of Retrovirus-related Pol polyprotein from transposon vs. TrEMBL
Match: A0A4Y2CA65 (Retrovirus-related Pol polyprotein from transposon 297 OS=Araneus ventricosus OX=182803 GN=pol_3947 PE=4 SV=1) HSP 1 Score: 132.88 bits (333), Expect = 3.476e-73 Identity = 65/171 (38.01%), Postives = 108/171 (63.16%), Query Frame = 1
Query: 391 LSKFGNIFMASKFDLGYTELVKHEIKTMGNP-VMQHPRRQPMHLEQKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLMHT 900
L + N+F D+G + +H I T +P + Q+PRR P+ +++ + L+K+++++ +I + W S +V + K+DGSTR C+DYR LN IT+K+S+ +P +LD L S++F+++DL+ +QVE+ PE +++TAF+T GQ GL PATF+RLM T
Sbjct: 234 LKELQNLFSTCDADVGRCNMTQHRINTGDHPPIKQYPRRLPLARKEEADNLVKEMVDNGIIEESSGPWASPIVLVKKKDGSTRFCVDYRKLNEITKKDSYPLPRIDDILDALNGSQWFTTLDLKSGYWQVEIRPEDREKTAFTT--GQ--------GLCNAPATFERLMET 394 HSP 2 Score: 125.946 bits (315), Expect = 3.476e-73 Identity = 64/148 (43.24%), Postives = 94/148 (63.51%), Query Frame = 3
Query: 879 FSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKILWTKECEESFETLKK 1322
F ++ +L GL ++ +VYLDDI+I G +EH N L +VF R+Q+A LK+NPKKC ++ E+ +LG+ I E GV+T+S KI+ + P +R FLGL YYRRF+K ++ IA PL+ T + WT ECE+ F +LK+
Sbjct: 388 FERLMETVLRGLSSEACLVYLDDIIIVGWTFEEHLNNLRKVFQRLQKANLKLNPKKCRFFQREVAYLGHVISE-GVKTDSEKIKAVVDWPRPDKIHDLRSFLGLCTYYRRFVKKFSTIARPLHKLTEA-KSNFNWTDECEKFFNSLKQ 533 HSP 3 Score: 69.707 bits (169), Expect = 3.476e-73 Identity = 54/157 (34.39%), Postives = 74/157 (47.13%), Query Frame = 2
Query: 1304 FRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQ--------------------------MKEDI----NT*HXXXXXXXXXXXARTDHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSRIN-GDVCAQCQTRHK 1681
F ++K+ L +PIL + R ++ FIL TDAS IGAVLSQ KE + + H + +K + RTDH +L +L K+ Q W+ L EYDFE+++RKG H NAD LSR + C QC K
Sbjct: 528 FNSLKQALTSSPILTYPRTDKDFILDTDASNEGIGAVLSQNTGNEERVIAYFSKSLGKPERNYCVTRKELLAIVKSIEHFHHYLYGRKFLLRTDHASLRWLLNFKEP-EGQIARWIQRLQEYDFEIQHRKGTSHGNADALSRRPCKESCKQCTNAEK 683
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Cavefish
Match: ENSAMXT00000052546.1 (pep primary_assembly:Astyanax_mexicanus-2.0:2:13031363:13036570:1 gene:ENSAMXG00000033629.1 transcript:ENSAMXT00000052546.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 126.716 bits (317), Expect = 1.104e-67 Identity = 67/169 (39.64%), Postives = 99/169 (58.58%), Query Frame = 1
Query: 391 LSKFGNIFMASKFDLGYTELVKHEIKTM-GNPVMQHPRRQPMHLEQKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLM 894
L K +IF S D GYT V H I T PV Q RR P + Q+ + ++ L++ ++ + S W S V + K+DG+ R C DYR LN +T K+++ +P LD L N+++FS++DL +QV V +++TA +T G F ++R+PFGL PATFQRLM
Sbjct: 691 LEKHKDIFSKSDSDFGYTTAVTHSIPTGDAPPVKQRHRRVPPQVFQEFKKHVQSLVDRGILEESCSPWASPAVIVIKKDGTVRFCCDYRRLNQVTCKDAYPLPRVEESLDALGNAQWFSTLDLTAGYFQVAVKDSDREKTAVTTPFGLFQWSRMPFGLCNAPATFQRLM 859 HSP 2 Score: 121.709 bits (304), Expect = 1.104e-67 Identity = 58/153 (37.91%), Postives = 94/153 (61.44%), Query Frame = 3
Query: 879 FSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKK------ILWTKECEESFETLK 1319
F ++ +L L F ++VYLDDI+I+ ++ + H LE VFNR++ GLK+ P KC + K E+ FLG+ I GV+ ++ K+ +++ +PK+ K IR LG +YYRRF+ +A++A PL+A G + I W+ EC+ +F+ LK
Sbjct: 855 FQRLMGVVLGDLAFDILLVYLDDIIIFSRDFKSHCERLELVFNRLRHHGLKLKPSKCFLLKPEVKFLGHIISAKGVQVDAEKVRVLETWPIPKTVKDIRQVLGFMSYYRRFVPKFAQLAKPLHALVGGKMNRGRATGPITWSGECQTAFDKLK 1007 HSP 3 Score: 50.8322 bits (120), Expect = 1.104e-67 Identity = 41/147 (27.89%), Postives = 60/147 (40.82%), Query Frame = 2
Query: 1304 FRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQM-------------------KEDIN---------------T*HXXXXXXXXXXXARTDHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSRI 1642
F +K L P+L + + FIL TD S +GAVLSQ K D N T K TDH L +L + + Q W+A ++EY+F++ Y+ G +++NAD LSR+
Sbjct: 1003 FDKLKGCLMSPPVLAYPDFTQPFILTTDGSLHGLGAVLSQKQGGTERVIAYASRGLRGSEKNDKNYSAFKLELLALKWAVTEKFRDFLMFAKFSVVTDHNPLRYLDSANLGVVEQ--RWVAQMAEYNFDISYKPGRQNANADVLSRL 1147
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Cavefish
Match: ENSAMXT00000047000.1 (pep primary_assembly:Astyanax_mexicanus-2.0:21:9985706:9990085:1 gene:ENSAMXG00000032330.1 transcript:ENSAMXT00000047000.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 119.398 bits (298), Expect = 2.650e-66 Identity = 64/178 (35.96%), Postives = 100/178 (56.18%), Query Frame = 1
Query: 376 RKHSNLSKFGNIFMASKFDLGYTELVKHEIKTMGNPVMQHPRRQPMHLE--QKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLMHTC 903
R LS +F + D G T VKH IK +H R +P+H + + V +++LL +IR+ +S ++S +V + K++G R+C+DYR LN T K+++ +P L SK+FS +DL+ YQ+EV + +TAF G + FNR+P G++ P+TFQRLM C
Sbjct: 320 RVTQKLSSLPEVFAHNDLDYGRTNRVKHHIKLSDETPFKH-RARPIHPQDLEAVRKHLQELLQAGIIRESESPFSSPIVVVRKKNGDVRLCIDYRKLNLQTVKDAYALPNLEETFSTLTGSKWFSVLDLKSGYYQIEVEETDKPKTAFVCPLGFWEFNRMPQGITNAPSTFQRLMEKC 496 HSP 2 Score: 116.316 bits (290), Expect = 2.650e-66 Identity = 58/167 (34.73%), Postives = 95/167 (56.89%), Query Frame = 3
Query: 879 FSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGC-----------EKK----------ILWTKECEESFETL 1316
F ++ + G+ K V+V+LDD++++ K +EH+ L +V NR+++ GLK++P KC ++T + +LG+ + + GV T+ K+E +K+ PK K++R FLG + YYRRFIK+Y+ + PL T G E K WT ECE SF+ +
Sbjct: 489 FQRLMEKCMSGMNLKEVLVFLDDLIVFSKTLEEHEVRLLKVLNRLKEYGLKLSPTKCKFFQTSVRYLGHIVSQDGVETDPNKVEALKTWPRPKGLKELRSFLGFSGYYRRFIKNYSSVVRPLTNLTVGYPPLRRSSQLKKEPKQYHDPKQPFGSRWTPECECSFQAI 655 HSP 3 Score: 58.9214 bits (141), Expect = 2.650e-66 Identity = 41/143 (28.67%), Postives = 66/143 (46.15%), Query Frame = 2
Query: 1304 FRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQMKED----INT*HXXXXXXXXXXXAR---------------------------TDHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSR 1639
F+ + ++L AP+L F+ ++L+TDAS +GA L Q +E I+ + K A TD L ++ TT K + + W+A LS ++F+LKYR G+++ +ADGLSR
Sbjct: 652 FQAIIEKLTSAPVLGFANPQLPYVLHTDASTTGLGAALYQEQEGKMRVISYASRGLSRSESKYPAHKLEFLALKWAVTEKFNDYLYGSPFTVVTDSNPLTYILTTAKMDATSYR-WLATLSTFNFKLKYRAGKQNGDADGLSR 793 HSP 4 Score: 46.9802 bits (110), Expect = 5.570e-6 Identity = 32/135 (23.70%), Postives = 67/135 (49.63%), Query Frame = 3
Query: 1743 ERIQEEQHKD-CKFQKVSEFLQDNTIQ---KKDLPK-KFLFKQIDYIRYEEGLLIMER-DG---SHLVLVPENYVEELVKQIHVELCHVGIKKLSHYISNIFFWPSIQDSIPECLRKCVECAKRKFDQKRTKEIF 2120
E IQ +Q D C + + + TI +++LP+ KQ +R +L +R DG ++ +++PE ++K +H ++ H+G+++ + + F+WP + D + ++ C C +RK +R +
Sbjct: 889 EEIQNQQRADPCLREIIHQMETGETISPAVREELPQVTHFLKQWKKLRLYNKILYRQRLDGDNPTYQLVLPEPSRAVVLKSLHDDMGHMGMERTLNLARSRFYWPKMSDDVERKIKTCDRCVRRKTLPERAAPLM 1023 HSP 5 Score: 23.483 bits (49), Expect = 5.570e-6 Identity = 11/29 (37.93%), Postives = 14/29 (48.28%), Query Frame = 2
Query: 2195 KYVVVIIDRFNKLVSLTATSKQDDQTIFR 2281
K V+VI D F K T Q QT+ +
Sbjct: 1049 KDVLVITDHFTKYAVAIPTPNQKAQTVAK 1077
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Cavefish
Match: ENSAMXT00000042297.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002193.1:21:2557:1 gene:ENSAMXG00000030761.1 transcript:ENSAMXT00000042297.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 128.642 bits (322), Expect = 3.126e-66 Identity = 68/185 (36.76%), Postives = 110/185 (59.46%), Query Frame = 1
Query: 355 MSGKTR*RKHSNLSKFGNIFMASKFDLGYTELVKHEIKTMGNPVMQHPRRQPMHLE--QKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLMHTC 903
+S + + R +NL+ +F D G+T V H I+ + +P R +P+H + V + +K+L + N+IR+ +SS+ S VV + K+ G+ R+C+DYR LN+ T K+++ +P L S++FS +DL+ YQVEV E + +TAF T G + FNRLP G++ P+TFQR+M C
Sbjct: 82 LSAEWKDRITANLNSIPEVFANGDLDYGHTTAVTHRIR-LSDPTPFKQRARPIHPSDYEAVRLHLKELQDANIIRESESSFASPVVVVKKKSGAIRLCVDYRKLNSQTIKDAYALPNIEETFSALHGSRWFSVMDLKSGYYQVEVEEEDKHKTAFVTPMGFWEFNRLPQGVTNAPSTFQRVMEKC 265 HSP 2 Score: 112.464 bits (280), Expect = 3.126e-66 Identity = 53/167 (31.74%), Postives = 94/167 (56.29%), Query Frame = 3
Query: 879 FSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATT---------------------TGCEKKILWTKECEESFETL 1316
F +V + + K V+V+LDD++++ +EH++ L V +++ GLK++P+KCH ++ + +LG+ + E GV T+ KI + + P++ ++++ FLG T YYRRF+KDY+KIA PL T +G + W ++C+E+F TL
Sbjct: 258 FQRVMEKCMGTMNLKEVLVFLDDLIVFSDTLEEHESRLMRVLTKLKDFGLKLSPEKCHFFRRSVRYLGHIVSEDGVETDPEKISALTTWPKPRNIRELKSFLGFTGYYRRFVKDYSKIARPLNDLTVGYIPQKRRSRGKPHQLVDQDSGGPLQEKWNEKCDEAFNTL 424 HSP 3 Score: 53.1434 bits (126), Expect = 3.126e-66 Identity = 38/143 (26.57%), Postives = 63/143 (44.06%), Query Frame = 2
Query: 1304 FRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQMKEDINT*HXXXXXXXXXXXARTDHKALVFLSTTKKAISPQF-------------------------------HTWMANLSEYDFELKYRKGEEHSNADGLSR 1639
F + ++L APIL F+ + ++L+TDAS +GA L Q ++ + + R L FL+ K A++ +F H W+A LS ++F ++YR G+ + +ADGLSR
Sbjct: 421 FNTLIEKLTTAPILGFANPHLPYVLHTDASVQGLGAALYQQQDGQLRVIAYASRGLSYSEKRYPTHKLEFLA-VKWAVTDKFFDYLYGAEFTIMTDNNPLTYVLTSAKLDAAGHRWLAALSTFNFSIRYRAGKRNQDADGLSR 562
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Cavefish
Match: ENSAMXT00000053730.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002271.1:240468:243660:-1 gene:ENSAMXG00000043631.1 transcript:ENSAMXT00000053730.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 128.257 bits (321), Expect = 4.488e-66 Identity = 68/185 (36.76%), Postives = 110/185 (59.46%), Query Frame = 1
Query: 355 MSGKTR*RKHSNLSKFGNIFMASKFDLGYTELVKHEIKTMGNPVMQHPRRQPMHLE--QKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLMHTC 903
+S + + R +NL+ +F D G+T V H I+ + +P R +P+H + V + +K+L + N+IR+ +SS+ S VV + K+ G+ R+C+DYR LN+ T K+++ +P L S++FS +DL+ YQVEV E + +TAF T G + FNRLP G++ P+TFQR+M C
Sbjct: 214 LSAEWKDRITANLNSIPEVFANGDLDYGHTTAVTHRIR-LSDPTPFKQRARPIHPSDYEAVRLHLKELQDANIIRESESSFASPVVVVKKKSGAIRLCVDYRKLNSQTIKDAYALPNIEETFSALHGSRWFSVMDLKSGYYQVEVEEEDKHKTAFVTPMGFWEFNRLPQGVTNAPSTFQRVMEKC 397 HSP 2 Score: 112.464 bits (280), Expect = 4.488e-66 Identity = 53/167 (31.74%), Postives = 94/167 (56.29%), Query Frame = 3
Query: 879 FSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATT---------------------TGCEKKILWTKECEESFETL 1316
F +V + + K V+V+LDD++++ +EH++ L V +++ GLK++P+KCH ++ + +LG+ + E GV T+ KI + + P++ ++++ FLG T YYRRF+KDY+KIA PL T +G + W ++C+E+F TL
Sbjct: 390 FQRVMEKCMGTMNLKEVLVFLDDLIVFSDTLEEHESRLMRVLTKLKDFGLKLSPEKCHFFRRSVRYLGHIVSEDGVETDPEKISALTTWPKPRNIRELKSFLGFTGYYRRFVKDYSKIARPLNDLTVGYIPQKRRSRGKPHQLVDQDSGGPLQEKWNEKCDEAFNTL 556 HSP 3 Score: 53.1434 bits (126), Expect = 4.488e-66 Identity = 38/143 (26.57%), Postives = 63/143 (44.06%), Query Frame = 2
Query: 1304 FRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQMKEDINT*HXXXXXXXXXXXARTDHKALVFLSTTKKAISPQF-------------------------------HTWMANLSEYDFELKYRKGEEHSNADGLSR 1639
F + ++L APIL F+ + ++L+TDAS +GA L Q ++ + + R L FL+ K A++ +F H W+A LS ++F ++YR G+ + +ADGLSR
Sbjct: 553 FNTLIEKLTTAPILGFANPHLPYVLHTDASVQGLGAALYQQQDGQLRVIAYASRGLSYSEKRYPTHKLEFLA-VKWAVTDKFFDYLYGAEFTIMTDNNPLTYVLTSAKLDAAGHRWLAALSTFNFSIRYRAGKRNQDADGLSR 694
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Cavefish
Match: ENSAMXT00000041682.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002320.1:24977:29035:1 gene:ENSAMXG00000038531.1 transcript:ENSAMXT00000041682.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 130.183 bits (326), Expect = 5.259e-66 Identity = 69/169 (40.83%), Postives = 104/169 (61.54%), Query Frame = 1
Query: 391 LSKFGNIFMASKFDLGYTELVKHEIKTMGN-PVMQHPRRQPMHLEQKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLM 894
L ++ ++F + +LG T L++HEI + + PV Q RR P V+ I++LL+ VIR S ++S VV + K DG+ R+C+DYR LN+ T K+++ +P LD L +++FS++DL QV +A + +TAF T G F FNR+PFGL P+TFQRLM
Sbjct: 293 LQRYSSVFSQDEGELGCTHLIEHEIPLIDDTPVKQRYRRLPPSQYDLVKGHIQELLDRKVIRASCSPYSSPVVVVQKRDGTIRLCVDYRQLNSKTRKDAYPLPRIEESLDALGGARFFSTLDLASGYNQVPMAEHDKSKTAFCTPFGLFEFNRMPFGLCNAPSTFQRLM 461 HSP 2 Score: 113.62 bits (283), Expect = 5.259e-66 Identity = 55/144 (38.19%), Postives = 89/144 (61.81%), Query Frame = 3
Query: 918 FKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLY---ATTTGCEKKI-------LWTKECEESFETLK 1319
F+S+++YLDDI+I+ H LE V R+QQ LK+ KCH +++++ +LG+ I +GV T+ KI+ + P++ Q+R FLG +YYRRF++ +AK A+PL+ A G ++++ W+ CEE+FETLK
Sbjct: 470 FQSLLLYLDDIVIFSSTFDLHLQRLEVVLKRLQQNNLKLKLSKCHFFQSQVKYLGHVISSAGVATDPEKIKAVSEWERPQTVTQLRSFLGFASYYRRFVEGFAKHASPLHRLVAVLQGGKRRVKTKPVEGHWSDACEEAFETLK 613 HSP 3 Score: 49.6766 bits (117), Expect = 5.259e-66 Identity = 41/147 (27.89%), Postives = 64/147 (43.54%), Query Frame = 2
Query: 1304 FRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQMKE------------------DINT*HXXXXXXXXXXXART----------------DHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSRI 1642
F +K +L AP+L ++ ++ FIL DAS +GAVLSQ +E +++ K + A T D+ L +L T K Q W++ L+ ++F +KYR G + NAD LSR+
Sbjct: 609 FETLKLKLVSAPVLGYADFSKPFILEIDASHGGLGAVLSQDQEGGRRPIAYASRGLRDSERNMSNYSSMKLELLGLKWAVTEKFREYLLGAQFTVYTDNNPLSYLQTAKLGAVEQ--RWVSQLALFNFNIKYRPGLSNRNADALSRL 753
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Medaka
Match: ENSORLT00000040305.1 (pep primary_assembly:ASM223467v1:1:25459511:25466649:-1 gene:ENSORLG00000028409.1 transcript:ENSORLT00000040305.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 135.961 bits (341), Expect = 2.457e-77 Identity = 69/178 (38.76%), Postives = 105/178 (58.99%), Query Frame = 1
Query: 364 KTR*RKHSNLSKFGNIFMASKFDLGYTELVKHEI-KTMGNPVMQHPRRQPMHLEQKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLM 894
++R R L + ++F A DLG T +V+H+I T G+ V Q PRR ++ + ++ L + R SSW + +V + K+D S R+C+DYR LN T K+++ +P + LD L +KYFS++DL +QVE+ P + AF T++G F +N +PFGL PATFQRLM
Sbjct: 792 ESRYRLAGLLRSYSDVFSAGSTDLGRTNVVQHDILTTPGSAVKQQPRRMSREKQEAADQQVQQSLEAGLARHSNSSWAAPIVMVKKKDQSPRLCVDYRPLNDRTIKDAYPLPRIQDTLDTLSTAKYFSTLDLTSGYWQVEMTPRARKAAAFCTRKGLFEWNVMPFGLCNAPATFQRLM 969 HSP 2 Score: 132.494 bits (332), Expect = 2.457e-77 Identity = 57/148 (38.51%), Postives = 99/148 (66.89%), Query Frame = 3
Query: 879 FSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKILWTKECEESFETLKK 1322
F ++ +L GL +++ +VYLDDI++ G N + LE+VF R++QA LK+ P KC +++ ++ +LG+ + G+ T+ +KI++++ +PK+ ++R F+GL +YYRRF+ D+A IA PL+ T + WT EC+E+FE LK+
Sbjct: 965 FQRLMDRVLAGLQWEACLVYLDDIIVLGCNSNQMMERLEQVFTRLRQANLKLKPSKCRLFQEQVAYLGHVVSAQGISTDPQKIQKVQDWPIPKNISEVRQFVGLASYYRRFVADFATIARPLHELTKK-YARFDWTTECQEAFEELKE 1111 HSP 3 Score: 63.5438 bits (153), Expect = 2.457e-77 Identity = 44/142 (30.99%), Postives = 66/142 (46.48%), Query Frame = 2
Query: 1304 FRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQMKEDIN------------------------------T*HXXXXXXXXXXXARTDHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSR 1639
F +K+RL APIL + ++ +L TDAS +GAVLSQ++ T H + + + RTDH +L +L+ K+ Q W+ L+EYDF++ +R G+ H NAD LSR
Sbjct: 1106 FEELKERLTSAPILGYPLDSGELLLDTDASDWGVGAVLSQVQGGEERVLAYGSRRLTTTEQNYCTTRRELLAVVEFTSHFRQYLLGRSFVLRTDHSSLRWLTRLKEPEG-QLARWLEKLAEYDFQVLHRPGKVHQNADALSR 1246
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Medaka
Match: ENSORLT00000040668.1 (pep primary_assembly:ASM223467v1:15:23416291:23420844:1 gene:ENSORLG00000029329.1 transcript:ENSORLT00000040668.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 127.487 bits (319), Expect = 1.397e-66 Identity = 72/191 (37.70%), Postives = 111/191 (58.12%), Query Frame = 1
Query: 343 KSIGMSGKTR*RKHSNLSKFGNIFMASKFDLGYTELVKHEIK-TMGNPVMQHPRRQPMHLEQKVEVL---IKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLMHTC 903
K +S + R R + L+ +F FD G TE VKH+IK + P Q PR P+H Q V+ + ++DLL +IR+ S + S +V + K+DG R+C+DYR LN+ T K+++ +P L SK+F+ +DL+ YQ+E+ E +++TAF G + FNR+P G++ P+TFQRLM C
Sbjct: 300 KDSPISPEWRQRIINKLNSIPEVFSRHDFDFGKTEKVKHKIKLSDPTPFKQRPR--PIH-PQDVDSVRNHLQDLLGSGIIRESTSPFASPIVVVRKKDGGVRLCIDYRKLNSQTIKDAYALPKLEDTFTALSGSKWFTVLDLKSGYYQIEMEEEDKEKTAFVCPLGFWEFNRMPQGITNAPSTFQRLMEKC 487 HSP 2 Score: 109.768 bits (273), Expect = 1.397e-66 Identity = 53/156 (33.97%), Postives = 91/156 (58.33%), Query Frame = 3
Query: 912 LIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGC------EKKIL---------------WTKECEESFETL 1316
L + V+V++DDI+++ +EH+ L V N++++ GLK++ KC ++T + +LG+ + + GVRT+ KI + S VPK+ K++R FLG YYRRF+K Y+ I PL+ T G +K ++ WT EC+++F+T+
Sbjct: 491 LNLREVLVFIDDIIVFAPTLEEHEERLMRVLNKLKEYGLKLSIDKCIFFQTSVKYLGHIVSQDGVRTDPEKISALTSWPVPKTLKELRSFLGFAGYYRRFVKGYSFIVKPLHDLTAGYPPSQKKQKPLIKKQEYHNPKEPFGGRWTVECQQAFDTI 646 HSP 3 Score: 58.151 bits (139), Expect = 1.397e-66 Identity = 39/143 (27.27%), Postives = 67/143 (46.85%), Query Frame = 2
Query: 1304 FRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQMKEDINT*HXXXXXXXXXXXAR-------------------------------TDHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSR 1639
F + ++L AP+L F+ + ++L+TDA +GAVL Q E N + + K +R TD L +L T+ K + + W++ LS +DF+++YR G+++++ADGLSR
Sbjct: 643 FDTIIQKLTSAPVLAFADPQKPYLLHTDACSTGLGAVLYQEHEGKNRVVAYASRGLTKSESRYPAHKLEFLALKWAVSEKFNDYLYGASFKVVTDSNPLTYLLTSAKLDATSYR-WLSALSTFDFKIEYRAGKQNTDADGLSR 784
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Medaka
Match: ENSORLT00000033726.1 (pep primary_assembly:ASM223467v1:14:24352555:24357012:-1 gene:ENSORLG00000024900.1 transcript:ENSORLT00000033726.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 127.872 bits (320), Expect = 1.893e-65 Identity = 72/191 (37.70%), Postives = 111/191 (58.12%), Query Frame = 1
Query: 343 KSIGMSGKTR*RKHSNLSKFGNIFMASKFDLGYTELVKHEIK-TMGNPVMQHPRRQPMHLEQKVEVL---IKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLMHTC 903
K +S + R R + L+ +F FD G TE VKH+IK + P Q PR P+H Q V+ + ++DLL +IR+ S + S +V + K+DG R+C+DYR LN+ T K+++ +P L SK+F+ +DL+ YQ+E+ E +++TAF G + FNR+P G++ P+TFQRLM C
Sbjct: 270 KDSPISPEWRQRIINKLNSIPEVFSRHDFDFGKTEKVKHKIKLSDPTPFKQRPR--PIH-PQDVDSVRNHLQDLLGSGIIRESTSPFASPIVVVRKKDGGVRLCIDYRKLNSQTIKDAYALPKLEDTFTALSGSKWFTVLDLKSGYYQIEMEEEDKEKTAFVCPLGFWEFNRMPQGITNAPSTFQRLMEKC 457 HSP 2 Score: 105.531 bits (262), Expect = 1.893e-65 Identity = 53/156 (33.97%), Postives = 91/156 (58.33%), Query Frame = 3
Query: 912 LIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGC------EKKIL---------------WTKECEESFETL 1316
L + V+V++DDI+++ +EH+ L V N++++ GLK++ KC ++T + +LG+ + + GVRT+ KI + S VPK+ K++R FLG YYRRF+K Y+ I PL+ T G +K ++ WT EC+++F+T+
Sbjct: 461 LNLREVLVFIDDIIVFAPTLEEHEERLR-VLNKLKEYGLKLSIDKCIFFQTSVKYLGHIVSQDGVRTDPEKISALTSWPVPKTLKELRSFLGFAGYYRRFVKGYSFIVKPLHDLTAGYPPSQKKQKPLIKKQEYHNPKEPFGGRWTVECQQAFDTI 615 HSP 3 Score: 58.151 bits (139), Expect = 1.893e-65 Identity = 39/143 (27.27%), Postives = 67/143 (46.85%), Query Frame = 2
Query: 1304 FRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQMKEDINT*HXXXXXXXXXXXAR-------------------------------TDHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSR 1639
F + ++L AP+L F+ + ++L+TDA +GAVL Q E N + + K +R TD L +L T+ K + + W++ LS +DF+++YR G+++++ADGLSR
Sbjct: 612 FDTIIQKLTSAPVLAFADPQKPYLLHTDACSTGLGAVLYQEHEGKNRVVAYASRGLTKSESRYPAHKLEFLALKWAVSEKFNDYLYGASFKVVTDSNPLTYLLTSAKLDATSYR-WLSALSTFDFKIEYRAGKQNTDADGLSR 753
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Medaka
Match: ENSORLT00000041485.1 (pep primary_assembly:ASM223467v1:15:30019002:30023522:-1 gene:ENSORLG00000028547.1 transcript:ENSORLT00000041485.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 119.013 bits (297), Expect = 1.768e-64 Identity = 63/178 (35.39%), Postives = 103/178 (57.87%), Query Frame = 1
Query: 376 RKHSNLSKFGNIFMASKFDLGYTELVKHEIKTMGNPVMQHPRRQPMHLE--QKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLMHTC 903
R + L++ ++F D G T +KH+I +H R +P+H + V I+DLL VIR+ +SS++S +V + K +G R+C+DYR LN T K+++ +P L SK+F+ +DL+ YQ+E+ + + +TAF T G + +NR+P G++ P+TFQRLM C
Sbjct: 282 RVKNKLTQMSDVFSHHDLDFGCTNQIKHQIHLHDPTPFKH-RARPIHPNDIEAVRKHIQDLLKAGVIRESESSFSSPIVVVRKRNGDVRLCIDYRKLNLQTIKDAYALPNLEESFSALTGSKWFTVLDLKSGYYQIEMDEKDRAKTAFVTPLGFWEWNRMPQGVTNAPSTFQRLMEKC 458 HSP 2 Score: 115.161 bits (287), Expect = 1.768e-64 Identity = 59/167 (35.33%), Postives = 92/167 (55.09%), Query Frame = 3
Query: 879 FSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCE--------KKIL-------------WTKECEESFETL 1316
F ++ + L + V+V+LDDI+++ +EH+ L +V R+++ GLK++P KC +++ + +LG+ + E GV T+ K+ +KS VP++ K++R FLG YYRRFIK YA I PL T G E KK WT C+ SFET+
Sbjct: 451 FQRLMEKCMGDLNLREVLVFLDDIIVFSATLEEHEERLLKVLKRLKEFGLKLSPDKCRFFQSSVRYLGHVVSERGVETDPEKVSTLKSWPVPRNLKELRSFLGFAGYYRRFIKSYATIVGPLNHLTRGYEPSRRTDKSKKTASKYTDSKQPFGDRWTPACQTSFETV 617 HSP 3 Score: 54.299 bits (129), Expect = 1.768e-64 Identity = 42/144 (29.17%), Postives = 64/144 (44.44%), Query Frame = 2
Query: 1304 FRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQMKE-DINT*HXXXXXXXXXXXARTDHKALVFLSTTKKAISPQFHT-------------------------------WMANLSEYDFELKYRKGEEHSNADGLSR 1639
F V +L AP+L F+ +IL+TDAS +GAVL Q +E + + HK L FL+ K +++ +FH W+A LS Y F+L+YR G+++++AD LSR
Sbjct: 614 FETVIDKLTSAPVLAFANPKLPYILHTDASLSGLGAVLYQEQEGQLRVIAYASRGLSQSESHYPAHK-LEFLA-LKWSVTEKFHDYLYGSHFTVITDNNPLTYILTSAKLDATSHRWLAALSTYSFKLQYRSGKQNNDADVLSR 755
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Medaka
Match: ENSORLT00000034816.1 (pep primary_assembly:ASM223467v1:11:17160839:17165359:-1 gene:ENSORLG00000030569.1 transcript:ENSORLT00000034816.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 119.013 bits (297), Expect = 1.827e-64 Identity = 63/178 (35.39%), Postives = 103/178 (57.87%), Query Frame = 1
Query: 376 RKHSNLSKFGNIFMASKFDLGYTELVKHEIKTMGNPVMQHPRRQPMHLE--QKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLMHTC 903
R + L++ ++F D G T +KH+I +H R +P+H + V I+DLL VIR+ +SS++S +V + K +G R+C+DYR LN T K+++ +P L SK+F+ +DL+ YQ+E+ + + +TAF T G + +NR+P G++ P+TFQRLM C
Sbjct: 282 RVKNKLTQMSDVFSHHDLDFGCTNQIKHQIHLHDPTPFKH-RARPIHPNDIEAVRKHIQDLLKAGVIRESESSFSSPIVVVRKRNGDVRLCIDYRKLNLQTIKDAYALPNLEESFSALTGSKWFTVLDLKSGYYQIEMDEKDRAKTAFVTPLGFWEWNRMPQGVTNAPSTFQRLMEKC 458 HSP 2 Score: 115.161 bits (287), Expect = 1.827e-64 Identity = 59/167 (35.33%), Postives = 92/167 (55.09%), Query Frame = 3
Query: 879 FSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCE--------KKIL-------------WTKECEESFETL 1316
F ++ + L + V+V+LDDI+++ +EH+ L +V R+++ GLK++P KC +++ + +LG+ + E GV T+ K+ +KS VP++ K++R FLG YYRRFIK YA I PL T G E KK WT C+ SFET+
Sbjct: 451 FQRLMEKCMGDLNLREVLVFLDDIIVFSATLEEHEERLLKVLKRLKEFGLKLSPDKCRFFQSSVRYLGHVVSERGVETDPEKVSTLKSWPVPRNLKELRSFLGFAGYYRRFIKSYATIVGPLNHLTRGYEPSRRTDKSKKTASKYTDSKQPFGDRWTPACQTSFETV 617 HSP 3 Score: 54.299 bits (129), Expect = 1.827e-64 Identity = 42/144 (29.17%), Postives = 64/144 (44.44%), Query Frame = 2
Query: 1304 FRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQMKE-DINT*HXXXXXXXXXXXARTDHKALVFLSTTKKAISPQFHT-------------------------------WMANLSEYDFELKYRKGEEHSNADGLSR 1639
F V +L AP+L F+ +IL+TDAS +GAVL Q +E + + HK L FL+ K +++ +FH W+A LS Y F+L+YR G+++++AD LSR
Sbjct: 614 FETVIDKLTSAPVLAFANPKLPYILHTDASLSGLGAVLYQEQEGQLRVIAYASRGLSQSESHYPAHK-LEFLA-LKWSVTEKFHDYLYGSHFTVITDNNPLTYILTSAKLDATSHRWLAALSTYSFKLQYRSGKQNNDADVLSR 755
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Planmine SMEST
Match: SMESG000081257.1 (SMESG000081257.1) HSP 1 Score: 226.868 bits (577), Expect = 1.619e-174 Identity = 106/169 (62.72%), Postives = 133/169 (78.70%), Query Frame = 1
Query: 391 LSKFGNIFMASKFDLGYTELVKHEIKTMGNPVMQHPRRQPMHLEQKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLMH 897
+S+F NIFMAS+FDLG+T+++ HE+KT G P++Q+PR QPMHLE KVE L+++LL V+RKC+S WN+ +V + K DGS R+CLD+R LN++TEK SF MP + LLDCL SK F SIDL QA Y VE+ Q +TAFSTKEGQFCFNRLPFGLS PATFQ+LMH
Sbjct: 422 ISQFSNIFMASRFDLGHTKVITHELKTSGPPILQNPRGQPMHLEAKVEELVQNLLEAGVVRKCQSPWNTPLVIVGKPDGSVRICLDFRLLNSVTEKFSFPMPDMQLLLDCLGKSKIFYSIDLGQAYYLVELNENSQIKTAFSTKEGQFCFNRLPFGLSTAPATFQKLMH 590 HSP 2 Score: 194.126 bits (492), Expect = 1.619e-174 Identity = 90/148 (60.81%), Postives = 116/148 (78.38%), Query Frame = 3
Query: 879 FSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKILWTKECEESFETLKK 1322
F K+ +LEGL+FK VVVYLDDILIYG+NQ+ HD +L EVF RI+ +GLK+NP+KC K+ L F+G+T+ E G++TN RKI EI++A PKS ++R FLGLT YYRRFIK+Y+ IAAPLYA TTGC+K I+WT+EC + F LKK
Sbjct: 585 FQKLMHQILEGLVFKGVVVYLDDILIYGENQETHDKLLFEVFTRIRDSGLKVNPEKCAFNKSVLNFIGHTVSEKGIQTNKRKISEIENATEPKSTTELRSFLGLTTYYRRFIKNYSMIAAPLYAATTGCDKMIVWTEECRKRFINLKK 732 HSP 3 Score: 141.354 bits (355), Expect = 1.619e-174 Identity = 76/154 (49.35%), Postives = 98/154 (63.64%), Query Frame = 2
Query: 1295 RGKFRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAV-------------LSQMKEDINT*HXXXXXXXXXXXARTDHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSRINGDVCAQCQTRHKDTKQEKPRVRHV 1717
R +F N+KK LCEAPIL + R +R+FI+ TD SF +IG + L + E I H + + ++ +ARTDHKAL F++TTKK ISPQF TWMAN SEYDF L+YRKG EH NA G SR+N +C+ Q HKD K+ K R R +
Sbjct: 724 RKRFINLKKLLCEAPILEYPRADRLFIIDTDDSFEAIGHLTKHEIGYCVTRKELLVLHEFIV--HFRQYLYGRRFVARTDHKALTFMNTTKKPISPQFQTWMANFSEYDFALQYRKGNEHGNAGGWSRLNNTICSHYQMEHKDAKKAKCRTRCI 875 HSP 4 Score: 115.931 bits (289), Expect = 1.619e-174 Identity = 59/129 (45.74%), Postives = 88/129 (68.22%), Query Frame = 2
Query: 14 VEMLWDSGASVTVKSEKIWKKIGSPRLTRSGIVLCGVFSANGEQPLRGITITARWFQKRRKFKVIVVKYIKPEFIGGIDTMDSFWIRLMEVNNIDANLVAQKHSEKERIEKVLVCLGKQDNESILTLVN 400
VEMLW+SGAS++V SEK W+ IGSP L S I+L VFS ++PL + I A+W +K R+ VI+V+ I P FIGG+DTM F + L EVNNI+++LV ++ ++ +R++ L+ L N + TL++
Sbjct: 295 VEMLWNSGASISVMSEKCWRLIGSPILMDSRILLSEVFSNEDKKPLGSVKIVAKWNKKFRELNVIIVRKIHPNFIGGVDTMKIFGMELKEVNNIESSLVNKRFTDSDRLKNTLLTLKLDKNSELGTLIS 423 HSP 5 Score: 107.457 bits (267), Expect = 9.738e-29 Identity = 49/106 (46.23%), Postives = 69/106 (65.09%), Query Frame = 3
Query: 1845 LFKQIDYIRYEEGLLIMERDGSHLVLVPENYVEELVKQIHVELCHVGIKKLSHYISNIFFWPSIQDSIPECLRKCVECAKRKFDQKRTKEIFLPRGSSEFLEQIFL 2162
+FK + ++ ++ +L++ DG V+VP++YV+ LV H+ELCH+GI K Y+ + FF PS+ I EC+ KC CA RK DQ RTKEI LPR FLEQI +
Sbjct: 921 IFKYLKILQIQDNVLMINTDGKLAVVVPDSYVKSLVNYFHIELCHLGINKTLFYLKDFFFLPSMNQIITECINKCKICASRKIDQGRTKEILLPRTGERFLEQIVV 1026 HSP 6 Score: 39.6614 bits (91), Expect = 9.738e-29 Identity = 21/41 (51.22%), Postives = 27/41 (65.85%), Query Frame = 2
Query: 2162 DIAYMNQRVGKKYVVVIIDRFNKLVSLTATSKQDDQTIFRI 2284
DI YM + KKY++VIID F+KLVSL QD+ TI +
Sbjct: 1027 DIDYMETKESKKYMIVIIDCFSKLVSLP----QDEATILNV 1063
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Planmine SMEST
Match: SMESG000065302.1 (SMESG000065302.1) HSP 1 Score: 175.637 bits (444), Expect = 2.011e-112 Identity = 91/169 (53.85%), Postives = 108/169 (63.91%), Query Frame = 1
Query: 391 LSKFGNIFMASKFDLGYTELVKHEIKTMGNPVMQHPRRQPMHLEQKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQRLMH 897
+S+F NIFMASKFDLG+ IK+LL V+RKC+SSWN+ +V + K DGS+RMCLD+R LN++TEK SF MP + LLDCL SK SSIDL Q YQVE+ Q +TAFSTKEGQFCFNRLPFGLS PATFQ+LMH
Sbjct: 182 ISQFSNIFMASKFDLGH---------------------------------IKNLLVAGVVRKCQSSWNTPLVIVGKPDGSSRMCLDFRCLNSVTEKVSFPMPDMQLLLDCLGKSKILSSIDLRQVYYQVELNENSQIKTAFSTKEGQFCFNRLPFGLSTAPATFQKLMH 317 HSP 2 Score: 162.155 bits (409), Expect = 2.011e-112 Identity = 78/139 (56.12%), Postives = 101/139 (72.66%), Query Frame = 3
Query: 879 FSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTGCEKKILWTKEC 1295
F K+ +LEGL+FK VVVYLDDIL Y +NQ+ HD +L EVF RI +GLK++P KC K+ L F+ +T+ E G++TN K EI + KS ++R FLGLTNYYRRFIK+Y+ IAAPLYA TTG +K I+WT+EC
Sbjct: 312 FQKLMHQILEGLVFKRVVVYLDDILKYAENQETHDKLLFEVFTRIIDSGLKVSPAKCAFNKSLLNFINHTVSEKGIQTNKGKFSEIVNPIETKSTTELRSFLGLTNYYRRFIKNYSMIAAPLYAATTGNDKMIVWTEEC 450 HSP 3 Score: 110.923 bits (276), Expect = 2.011e-112 Identity = 59/130 (45.38%), Postives = 88/130 (67.69%), Query Frame = 2
Query: 11 EVEMLWDSGASVTVKSEKIWKKIGSPRLTRSGIVLCGVFSANGEQPLRGITITARWFQKRRKFKVIVVKYIKPEFIGGIDTMDSFWIRLMEVNNIDANLVAQKHSEKERIEKVLVCLGKQDNESILTLVN 400
+EMLWDSGAS++V SEK W+ IGSP L S I L GVFS E+P + I A+ +K R+F VI+V+ I P+FIGG+DTM F ++L EVN I+++LV ++ ++ +R++ L+ L N + TL++
Sbjct: 54 HLEMLWDSGASISVMSEKCWRLIGSPILMDSRIRLSGVFSKEYEKPFGSVKIVAKRNKKVREFNVIIVRKIHPDFIGGVDTMKIFCMKLKEVNIIESSLVNKRFTDSDRLKNTLLTLKLDKNSKLGTLIS 183 HSP 4 Score: 95.9005 bits (237), Expect = 4.017e-39 Identity = 50/145 (34.48%), Postives = 83/145 (57.24%), Query Frame = 3
Query: 1731 TETMERIQEEQHKD-CKFQKVSEFLQDNTIQKKDLPKKFLFKQIDYIRYEEGLLIMERDGSHLVLVPENYVEELVKQIHVELCHVGIKKLSHYISNIFFWPSIQDSIPECLRKCVECAKRKFDQKRTKEIFLPRGSSEFLEQIFL 2162
+ M+ I+++Q++D + +S F+ + + +FK + ++ ++ +L++ DG V+VP++YV+ LV ++E ++GIKK + FFWPS+ I EC+ KC CA RK DQ R KEI PR FLEQI +
Sbjct: 575 SNIMKIIKQKQNEDRVTSEIISHFIGNEAHISYETISSSIFKYLKILQIQDDVLMINSDGKLAVVVPDSYVKSLVNYFYIEQGYLGIKKTLFCLKGFFFWPSMNQIITECINKCKICASRKIDQGRRKEILFPRTGERFLEQIIV 719 HSP 5 Score: 55.0694 bits (131), Expect = 4.017e-39 Identity = 20/39 (51.28%), Postives = 29/39 (74.36%), Query Frame = 2
Query: 1601 KGEEHSNADGLSRINGDVCAQCQTRHKDTKQEKPRVRHV 1717
KGEEH N+DG+SR+N +C+ CQ KD+K+ K R R++
Sbjct: 530 KGEEHGNSDGISRLNNTICSHCQIEQKDSKEGKCRTRYI 568 HSP 6 Score: 51.6026 bits (122), Expect = 4.017e-39 Identity = 23/41 (56.10%), Postives = 31/41 (75.61%), Query Frame = 2
Query: 2162 DIAYMNQRVGKKYVVVIIDRFNKLVSLTATSKQDDQTIFRI 2284
DIAYM + KKY++VIIDRF+KL+SL+A QD+ TI +
Sbjct: 720 DIAYMETKESKKYMIVIIDRFSKLISLSAAITQDEATILNV 760
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Planmine SMEST
Match: SMESG000035021.1 (SMESG000035021.1) HSP 1 Score: 146.747 bits (369), Expect = 9.462e-106 Identity = 70/128 (54.69%), Postives = 94/128 (73.44%), Query Frame = 3
Query: 879 FSKVDAYMLEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTG 1262
F K L+GL+FK V+YLDDILIY +NQ+ +D +L EVF RI+ +GLK NP+KC K+ L F+G+T+ E G++TN RKI EI++A PKS ++R FLGLTN+Y+RFIK+Y+ IA PLY TT
Sbjct: 270 FQKHINQTLKGLVFKGEVLYLDDILIYAENQETYDKLLFEVFIRIRDSGLKANPEKCAFNKSVLNFIGHTVSEKGIQTNKRKISEIENATEPKSTTELRSFLGLTNFYQRFIKNYSMIAEPLYTATTA 397 HSP 2 Score: 132.109 bits (331), Expect = 9.462e-106 Identity = 71/164 (43.29%), Postives = 95/164 (57.93%), Query Frame = 1
Query: 397 KFGNIFMASKFDLGYTELVKHEIKTMGNPVMQHPRRQPMHLEQKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQR 888
+F NIFMAS+FD G+ +++ HEIKT +P++Q+ RRQ MHLE KVE L+++LL V+RK +S WN+ +V + K D S + S++ QA YQVE+ Q +TAFSTKEGQ CFNRLP GLS PATFQ+
Sbjct: 146 QFSNIFMASRFDFGHIKVIAHEIKTSDSPILQNSRRQLMHLEGKVEELVQNLLEAGVLRKSQSPWNTPLVIVGKPDDSKNV------------------------------SRF-------QAYYQVELNENSQIKTAFSTKEGQLCFNRLPIGLSTAPATFQK 272 HSP 3 Score: 104.375 bits (259), Expect = 9.462e-106 Identity = 56/108 (51.85%), Postives = 74/108 (68.52%), Query Frame = 2
Query: 11 EVEMLWDSGASVTVKSEKIWKKIGSPRLTRSGIVLCGVFSANGEQPLRGITITARWFQKRRKFKVIVVKYIKPEFIGGIDTMDSFWIRLMEVNNIDANLVAQKHSEKE 334
VEMLWDSGAS++V SEK W+ IGSP L S I L GVF E+ L G I A+W +K RK VI+V+ I P+FIGG+DT+ F + L EVNNI++ LV ++ ++ E
Sbjct: 17 HVEMLWDSGASISVISEKCWRLIGSPILMDSRIRLSGVFPKEDEK-LLGSKIVAKWNKKFRKLNVIIVRKIYPDFIGGVDTIKIFGMELKEVNNIESLLVNKRFTDSE 123 HSP 4 Score: 64.3142 bits (155), Expect = 9.462e-106 Identity = 41/104 (39.42%), Postives = 53/104 (50.96%), Query Frame = 2
Query: 1334 APILNFSRENRMFILYTDASFCSIGAVLSQMKEDINT-------------------------------*HXXXXXXXXXXXARTDHKALVFLSTTKKAISPQFH 1552
APIL + R +R+FI+ TD SF +IGAVLSQ+KED H + + K+ +AR DHKAL F++TTKK IS QFH
Sbjct: 397 APILEYPRADRLFIIDTDDSFGAIGAVLSQIKEDGTEGVIAYGSRHLTEHEMEYCMTRKELFALHEFIVHFRQYLYGKRFVARMDHKALTFMNTTKKPISSQFH 500
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Planmine SMEST
Match: SMESG000035021.1 (SMESG000035021.1) HSP 1 Score: 149.828 bits (377), Expect = 7.097e-94 Identity = 69/120 (57.50%), Postives = 93/120 (77.50%), Query Frame = 3
Query: 903 LEGLIFKSVVVYLDDILIYGKNQQEHDNILEEVFNRIQQAGLKINPKKCHIYKTELVFLGYTIKESGVRTNSRKIEEIKSAAVPKSAKQIR*FLGLTNYYRRFIKDYAKIAAPLYATTTG 1262
L+GL+FK V+YLDDILIY +NQ+ +D +L EVF RI+ +GLK NP+KC K+ L F+G+T+ E G++TN RKI EI++A PKS ++R FLGLTN+Y+RFIK+Y+ IA PLY TTG
Sbjct: 278 LKGLVFKGEVLYLDDILIYAENQETYDKLLFEVFIRIRDSGLKANPEKCAFNKSVLNFIGHTVSEKGIQTNKRKISEIENATEPKSTTELRSFLGLTNFYQRFIKNYSMIAEPLYTATTG 397 HSP 2 Score: 132.494 bits (332), Expect = 7.097e-94 Identity = 71/164 (43.29%), Postives = 95/164 (57.93%), Query Frame = 1
Query: 397 KFGNIFMASKFDLGYTELVKHEIKTMGNPVMQHPRRQPMHLEQKVEVLIKDLLNDNVIRKCKSSWNSRVVKINKEDGSTRMCLDYRGLNAITEKESFTMPATRTLLDCLVNSKYFSSIDLEQA*YQVEVAPEVQDRTAFSTKEGQFCFNRLPFGLSITPATFQR 888
+F NIFMAS+FD G+ +++ HEIKT +P++Q+ RRQ MHLE KVE L+++LL V+RK +S WN+ +V + K D S + S++ QA YQVE+ Q +TAFSTKEGQ CFNRLP GLS PATFQ+
Sbjct: 146 QFSNIFMASRFDFGHIKVIAHEIKTSDSPILQNSRRQLMHLEGKVEELVQNLLEAGVLRKSQSPWNTPLVIVGKPDDSKNV------------------------------SRF-------QAYYQVELNENSQIKTAFSTKEGQLCFNRLPIGLSTAPATFQK 272 HSP 3 Score: 103.99 bits (258), Expect = 7.097e-94 Identity = 56/108 (51.85%), Postives = 74/108 (68.52%), Query Frame = 2
Query: 11 EVEMLWDSGASVTVKSEKIWKKIGSPRLTRSGIVLCGVFSANGEQPLRGITITARWFQKRRKFKVIVVKYIKPEFIGGIDTMDSFWIRLMEVNNIDANLVAQKHSEKE 334
VEMLWDSGAS++V SEK W+ IGSP L S I L GVF E+ L G I A+W +K RK VI+V+ I P+FIGG+DT+ F + L EVNNI++ LV ++ ++ E
Sbjct: 17 HVEMLWDSGASISVISEKCWRLIGSPILMDSRIRLSGVFPKEDEK-LLGSKIVAKWNKKFRKLNVIIVRKIYPDFIGGVDTIKIFGMELKEVNNIESLLVNKRFTDSE 123
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Planmine SMEST
Match: SMESG000049233.1 (SMESG000049233.1) HSP 1 Score: 182.956 bits (463), Expect = 6.879e-92 Identity = 91/157 (57.96%), Postives = 114/157 (72.61%), Query Frame = 2
Query: 1304 FRNVKKRLCEAPILNFSRENRMFILYTDASFCSIGAVLSQMKED---------------INT*HXXXXXXXXXXXARTDHKALVFLSTTKKAISPQFHTWMANLSEYDFELKYRKGEEHSNADGLSRINGDVCAQCQTRHKDTKQEKPRVRHVCFAK 1729
FR KK +CEAPIL++ RENR+F L TDASF IG+VLSQ+KED +N + + + K + RTDHKAL+FL+TTKKAISPQF TWM NLS+YDFELKY KGE+HSNADGLSRI G +C+QCQTRH++ K+ K RV+H+C +
Sbjct: 157 FRFSKKIMCEAPILDYPRENRIFNLNTDASFGEIGSVLSQLKEDGYCVTRKELLAIHEYVN--YFKQYLYGKLFMVRTDHKALIFLNTTKKAISPQFQTWMVNLSDYDFELKYHKGEDHSNADGLSRIGGMLCSQCQTRHENAKEAKVRVKHICMIQ 311 HSP 2 Score: 129.798 bits (325), Expect = 6.879e-92 Identity = 64/150 (42.67%), Postives = 103/150 (68.67%), Query Frame = 3
Query: 1713 MCVLQKTETMERIQEEQHKDCKFQKVSEFLQDNTIQKKDLPKKFLFKQIDYIRYEEGLLIMERDGSHLVLVPENYVEELVKQIHVELCHVGIKKLSHYISNIFFWPSIQDSIPECLRKCVECAKRKFDQKRTKEIFLPRGSSEFLEQIFL 2162
+C++QKTE M ++ E Q + +F+K+ + + + D F + D ++ +GLLI+ ++G L+LVP + ++LV +IH ++C VG+KKL HYI+NIF+WP++Q +I ECL+KC ECA+RK +Q++TKEI LP SEF +QI +
Sbjct: 307 ICMIQKTEVMHQLFEAQESNKQFEKMKKCVNNGNFGDFDKKDFFFKLR-DNLKMNDGLLIVNKNGMDLLLVPIEFTQQLVTKIHEKMCRVGVKKLVHYINNIFYWPNLQSTIKECLQKCQECARRKVNQEQTKEILLPHVISEFSDQIVI 455 HSP 3 Score: 53.1434 bits (126), Expect = 6.879e-92 Identity = 28/42 (66.67%), Postives = 32/42 (76.19%), Query Frame = 2
Query: 2159 FDIAYMNQRVGKKYVVVIIDRFNKLVSLTATSKQDDQTIFRI 2284
DIAYM KKY VVI D F+KLVSLTATSKQDD+ I+R+
Sbjct: 455 IDIAYMKPIATKKYAVVIKD-FSKLVSLTATSKQDDKAIYRV 495 HSP 4 Score: 35.4242 bits (80), Expect = 6.879e-92 Identity = 18/40 (45.00%), Postives = 22/40 (55.00%), Query Frame = 3
Query: 1203 RRFIKDYAKIAAPLYATTTGCEKKILWTKECEESFETLKK 1322
+RFI DY KIA PLYA C+K++ K F KK
Sbjct: 123 KRFINDYEKIAVPLYAAIELCKKRLPGRKNAMMLFRFSKK 162 HSP 5 Score: 74.7146 bits (182), Expect = 6.294e-20 Identity = 39/105 (37.14%), Postives = 57/105 (54.29%), Query Frame = 2
Query: 71 KKIGSPRLTRSGIVLCGVFSANGEQPLRGITITARWFQKRRKFKVIVVKYIKPEFIGGIDTMDSFWIRLMEVNNIDANLVAQKHSEKERIEKVLVCLGKQDNESI 385
+K+G P SGI L GVFS+ E+ L ++ W K FIGG+DTM+ F I+++E+NNI+ LV Q +++ ERI+K + L K N I
Sbjct: 3 EKVGKPSFGESGITLQGVFSSGNEKQLGSTVVSVLWNGK---------------FIGGVDTMELFGIKILEINNIEETLVNQNYTDLERIKKAMSFLNKGQNLKI 92 HSP 6 Score: 42.743 bits (99), Expect = 6.294e-20 Identity = 19/26 (73.08%), Postives = 21/26 (80.77%), Query Frame = 1
Query: 391 LSKFGNIFMASKFDLGYTELVKHEIK 468
L KFGNIFMASKFDL +T L HE+K
Sbjct: 96 LVKFGNIFMASKFDLRFTNLASHEMK 121 The following BLAST results are available for this feature:
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 2
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 2
BLAST of Retrovirus-related Pol polyprotein from transposon vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Retrovirus-related Pol polyprotein from transposon vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Retrovirus-related Pol polyprotein from transposon vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30012651 ID=SMED30012651|Name=Retrovirus-related Pol polyprotein from transposon|organism=Schmidtea mediterranea sexual|type=transcript|length=2284bpback to top protein sequence of SMED30012651-orf-1 >SMED30012651-orf-1 ID=SMED30012651-orf-1|Name=SMED30012651-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=118bp MASKFDLGYTELVKHEIKTMGNPVMQHPRRQPMHLEQKVEVLIKDLLNDNback to top protein sequence of SMED30012651-orf-2 >SMED30012651-orf-2 ID=SMED30012651-orf-2|Name=SMED30012651-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=150bp VEVEVEMLWDSGASVTVKSEKIWKKIGSPRLTRSGIVLCGVFSANGEQPLback to top protein sequence of SMED30012651-orf-3 >SMED30012651-orf-3 ID=SMED30012651-orf-3|Name=SMED30012651-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=183bp MRMDCQELTEMCVHNVRRDIKIPNKKNPEFDMCVLQKTETMERIQEEQHKback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|
