DUF4200 domain-containing protein

Overview
NameDUF4200 domain-containing protein
Smed IDSMED30012638
Length (bp)1125
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DUF4200 domain-containing protein (SMED30012638) t-SNE clustered cells

Violin plots show distribution of expression levels for DUF4200 domain-containing protein (SMED30012638) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DUF4200 domain-containing protein (SMED30012638) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DUF4200 domain-containing protein (SMED30012638) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012638

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30012638SMESG000028991.1 dd_Smed_v4_11371_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30012638SMESG000028991.1 dd_Smed_v4_11371_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30012638SMESG000028991.1 dd_Smed_v6_11371_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
neuronSMED30012638SMESG000028991.1 dd_Smed_v6_11371_0_1dd_Smed_v6PMID:30399335
Ross et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30012638SMESG000028991.1 dd_Smed_v4_11371_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of DUF4200 domain-containing protein vs. Ensembl Zebrafish
Match: si:ch1073-416d2.4 (si:ch1073-416d2.4 [Source:ZFIN;Acc:ZDB-GENE-131121-564])

HSP 1 Score: 110.923 bits (276), Expect = 6.396e-27
Identity = 84/289 (29.07%), Postives = 156/289 (53.98%), Query Frame = 1 
Query:   28 KNVFVTQ-----INEREDENSDRVVRHAIVKESPAHLLKSGINTFQSTLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMR-QQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTL-----FSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTEILNFGLIKRAVDNITKKC 861
            +NVFVTQ       ERE +   ++    I+ +SP+ +L+SG+ T Q TLV+KK  EL++I+T L + R + +++M    T++ ELL R Q+    + + F  F++EN+ KRRRA+ K Q E +  +  ++   +L  +++  ++   +L ++V  YK +EEFL+K +E LP +Y+ +  +++  +I RY TL         ++  +    K++ NQ     L  LK+ H    L  N  LS +  + + + ++ ++ E   +      R ++   G +  AV N+ ++C
Sbjct:   38 RNVFVTQPQPEESRERESDGEPQLKLIPIITQSPSGILESGVRTLQCTLVLKKREELDNINTHLTHTRAQIQQRMRSL-TERRELLQRAQEESRQRAERFQRFVEENELKRRRAMKKLQMEKQQSQSKEEERGELANQMERLQDRRMRLKQRVSRYKIFEEFLMKTLELLPDNYLGLGTDLVAPIIRRYETLSISRQDLLQQLQRLTDQMKTDHNQ-----LDTLKQQHNTYKLTTNQDLSDLQTQLDQMTERNKQQEMSLQLQQAQSRDQVEELGRVLLAVRNLAEQC 320
[Close]
BLAST of DUF4200 domain-containing protein vs. Ensembl Zebrafish
Match: si:ch1073-416d2.4 (si:ch1073-416d2.4 [Source:ZFIN;Acc:ZDB-GENE-131121-564])

HSP 1 Score: 88.9669 bits (219), Expect = 8.980e-20
Identity = 60/171 (35.09%), Postives = 104/171 (60.82%), Query Frame = 1 
Query:   28 KNVFVTQIN-----EREDENSDRVVRHAIVKESPAHLLKSGINTFQSTLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMR-QQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLP 522
            +NVFVTQ       ERE +   ++    I+ +SP+ +L+SG+ T Q TLV+KK  EL++I+T L + R + +++M    T++ ELL R Q+    + + F  F++EN+ KRRRA+ K Q E +  +  ++   +L  +++  ++   +L ++V  YK +EEFL+K +E LP
Sbjct:   33 RNVFVTQPQPEESRERESDGEPQLKLIPIITQSPSGILESGVRTLQCTLVLKKREELDNINTHLTHTRAQIQQRMRSL-TERRELLQRAQEESRQRAERFQRFVEENELKRRRAMKKLQMEKQQSQSKEEERGELANQMERLQDRRMRLKQRVSRYKIFEEFLMKTLELLP 202
[Close]
BLAST of DUF4200 domain-containing protein vs. Ensembl Xenopus
Match: klhl13 (kelch like family member 13 [Source:Xenbase;Acc:XB-GENE-923144])

HSP 1 Score: 51.9878 bits (123), Expect = 6.420e-7
Identity = 90/329 (27.36%), Postives = 146/329 (44.38%), Query Frame = 1 
Query:  142 TFQSTLVIK---KEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAF--------------LDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFST-NEMLMVDMTAKSEENQRFVQ----LLTKLKEDHARQLLQNNYRLSKM---YEKYETLKDQYE-RGEQIFKTNH------GDIRTEILNFGLIKRAVDNITKKCASKFDESIFENEIEIKLEKIQNYVITRRQIIEKVNHI-KDKPANNLPPNQPKTIQTN 1029
             F+  L++K   +E +  +  T L  KR E  +     +TQKEE  M+ ++                D    FD F+KEND+KR+RAL K   E +L    ++    LQ E     +    LL++ +   KY+++L +V+E    ++ +V E     +I R+NTL +T N++L  D+     ENQ   +     L   +E+   Q+L+ N +++++    E+   +  Q+E R  QI  T        G IR   LN          I+K+   K + S+   + E +LEKIQ          E ++ I KD       P  P    TN
Sbjct:   12 AFEDKLLVKMPDREDDFLTPATRLLEKRREMVEVEQALSTQKEEFQMKSESLQQRRAELELKEEKLKDSLFKFDKFLKENDSKRKRALHKAAEERQLAAHKEREALRLQAENTQLMQRKGTLLERQEKNSKYQQYLQRVLER-TDEFQEVQE-----MIDRFNTLMATQNKLLKRDL-----ENQELAEREKARLLHYQEETRSQILELNNQIAQLQGELERVRAVAFQWESRWAQIQNTAAENTLRLGRIRMSTLNL------FQTISKQMRLKTEISV--EDTEAQLEKIQI-------CFEDLSAIYKDLKKAGTTPQTPAVPTTN 314
[Close]
BLAST of DUF4200 domain-containing protein vs. Ensembl Mouse
Match: Ccdc42 (coiled-coil domain containing 42 [Source:MGI Symbol;Acc:MGI:3045254])

HSP 1 Score: 52.7582 bits (125), Expect = 2.539e-7
Identity = 61/244 (25.00%), Postives = 118/244 (48.36%), Query Frame = 1 
Query:  151 STLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTEILNFGLIKRAVDNITKKCASKFDES 882
            S  +++K+ E   +   + +K+  ++++M   N + EEL ++++      + F+ FI+END KR RAL K   E  L  L  + +   + E+   +  H+KL  K+Q Y  + ++L KV+E    ++ ++ E     +I RY TL S +  LM       E+ +R    L +  E+   ++LQ+N  L+++  +++  +      E  +         + L  G IK A  N+ +  + +  ES
Sbjct:   41 SIQLLEKKKEAKIMQEAMEHKKEAFQRRMETLNLRWEELGIKEEQLKAHIQKFEQFIQENDQKRIRALKKANKERELKRLRLRELAKAKQEMMALRLEHQKLSVKLQDYAIFNKYLEKVVE--NSEFEEIHE-----VIARYKTLVSMHHDLMQSAQEGQEKIERAKARLARYMEEKDDEILQHNNELARLQMRFDRARSDVIFWESRWAHIQNTAAKKTLLLGTIKMATLNLFQIVSKQLKES 277
[Close]
BLAST of DUF4200 domain-containing protein vs. UniProt/SwissProt
Match: sp|A9UQN0|CCD42_MONBE (Coiled-coil domain-containing protein 42 homolog OS=Monosiga brevicollis OX=81824 GN=30998 PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 4.698e-8
Identity = 61/255 (23.92%), Postives = 121/255 (47.45%), Query Frame = 1 
Query:   82 VVRHAIVKESPAHLLKSGINTFQSTLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVI-EYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQ----RFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTEILNFGLIK 831
             V   ++++ P  +L+    T  +T +++K  EL  ++  L  ++ ++  +M+  + +K EL  ++    +    FD F+KENDA+R RA  K  +E  + +  ++ +++L+ E+++ +E   +    +Q Y  +E F+  V+ EY  P+Y     N +  +I RY TL +T +    D+ ++   NQ    R  + + + KE H   +L  + +L+ +    E    +    E       G      L  G IK
Sbjct:   11 TVEEKLLQQMPDDVLQ---QTTPATQLLEKRRELEQVERALATQKEDFHVRMATLDQRKAELERKEYQLQESLLKFDRFLKENDARRERAEQKAASENEVAQAREEQVQNLRAELRSLQERKTRNRDILQRYSIFEAFMQTVLGEY--PEY-----NEVQDVITRYKTLVATQQ----DLRSRDRNNQTEIERVRREMARYKEAHRVSMLDCSNKLATLRTAKERAHTETLYWENQLSAVQGAASKRTLLLGQIK 251
[Close]
BLAST of DUF4200 domain-containing protein vs. UniProt/SwissProt
Match: sp|A7S8T5|CCD42_NEMVE (Coiled-coil domain-containing protein 42 homolog OS=Nematostella vectensis OX=45351 GN=v1g187067 PE=3 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 3.489e-7
Identity = 62/261 (23.75%), Postives = 126/261 (48.28%), Query Frame = 1 
Query:  151 STLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTEILNFGLIKRAVDNITKKCASKFDESIFENEIEIKLEKIQNYV 933
            +T +++K  E++ ++  L  ++ E++ KM     ++EEL  ++    +    FD F+KEND+KR RAL K Q E  +       I  L+ +     +  +K+  +++ Y  Y+++L KV+E    ++ ++ E     +I RY+TL +T++ L+       E+ ++    L K  E+   ++L  N +L+ +  K E  +    + E  +         + L  G IK A  N+         ++   +  + +L+KIQ ++
Sbjct:   32 ATRLLEKRREMSEVEQALGAQKEEFQMKMESLQQRREELERKEYQLRESLLKFDKFLKENDSKRARALKKAQEERDMRRAKDCEIARLKEDTSGLMKGRDKVQHRLEKYIIYQQYLEKVLEN-AEEFQEIRE-----IIARYDTLTATHQDLLERELKNQEKYEKEKARLIKFTEEKNNEILNYNNQLANLQTKLEKTQSVAVKWESQWTHIKNTAAKKTLLLGRIKMATHNLFMLVNRHLGQTGMVDMTDKQLDKIQVFI 286
[Close]
BLAST of DUF4200 domain-containing protein vs. UniProt/SwissProt
Match: sp|A6QQM8|CCD42_BOVIN (Coiled-coil domain-containing protein 42 OS=Bos taurus OX=9913 GN=CCDC42 PE=2 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 8.863e-7
Identity = 60/246 (24.39%), Postives = 117/246 (47.56%), Query Frame = 1 
Query:  151 STLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTEILNFGLIKRAVDNITKKCASKFDESIF 888
            S  +++K+ E   +   +  K+  ++++M   N + EEL +++       + F+ FI+END KR RAL K   E  L     + +   + E+   +  H++L  K+Q Y  + ++L KV+E    ++ ++ E     +I RY TL S +  LM       E+ +R    L + KE+   ++LQ+N  L+++  +++  +      E  +         + L  G IK A  N+ +  + +  E+ F
Sbjct:   41 SVRLLEKKKEAKIMHHAMEQKKETFQRRMETLNLRWEELGIKEAQLKAHIQKFEQFIQENDQKRIRALKKANKERELKRQRMRELAKAKQEMAVLRLEHQRLSAKLQEYSIFNKYLEKVVE--NSEFEEIHE-----VIARYKTLVSMHHDLMQSAQEGQEKIERAKARLARYKEEKDDEILQHNNELARLQMRFDRARSDVIIWESRWAHIQNTAAKKTLLLGTIKMATLNLFQIVSKQLKEATF 279
[Close]
BLAST of DUF4200 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q5SV66|CCD42_MOUSE (Coiled-coil domain-containing protein 42 OS=Mus musculus OX=10090 GN=Ccdc42 PE=2 SV=2)

HSP 1 Score: 52.7582 bits (125), Expect = 1.776e-6
Identity = 61/244 (25.00%), Postives = 118/244 (48.36%), Query Frame = 1 
Query:  151 STLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTEILNFGLIKRAVDNITKKCASKFDES 882
            S  +++K+ E   +   + +K+  ++++M   N + EEL ++++      + F+ FI+END KR RAL K   E  L  L  + +   + E+   +  H+KL  K+Q Y  + ++L KV+E    ++ ++ E     +I RY TL S +  LM       E+ +R    L +  E+   ++LQ+N  L+++  +++  +      E  +         + L  G IK A  N+ +  + +  ES
Sbjct:   41 SIQLLEKKKEAKIMQEAMEHKKEAFQRRMETLNLRWEELGIKEEQLKAHIQKFEQFIQENDQKRIRALKKANKERELKRLRLRELAKAKQEMMALRLEHQKLSVKLQDYAIFNKYLEKVVE--NSEFEEIHE-----VIARYKTLVSMHHDLMQSAQEGQEKIERAKARLARYMEEKDDEILQHNNELARLQMRFDRARSDVIFWESRWAHIQNTAAKKTLLLGTIKMATLNLFQIVSKQLKES 277
[Close]
BLAST of DUF4200 domain-containing protein vs. UniProt/SwissProt
Match: sp|B0BMJ2|CC42M_XENTR (Coiled-coil domain-containing protein 42 like-2 OS=Xenopus tropicalis OX=8364 GN=TNeu058c09.1 PE=2 SV=2)

HSP 1 Score: 51.9878 bits (123), Expect = 3.401e-6
Identity = 90/329 (27.36%), Postives = 146/329 (44.38%), Query Frame = 1 
Query:  142 TFQSTLVIK---KEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAF--------------LDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFST-NEMLMVDMTAKSEENQRFVQ----LLTKLKEDHARQLLQNNYRLSKM---YEKYETLKDQYE-RGEQIFKTNH------GDIRTEILNFGLIKRAVDNITKKCASKFDESIFENEIEIKLEKIQNYVITRRQIIEKVNHI-KDKPANNLPPNQPKTIQTN 1029
             F+  L++K   +E +  +  T L  KR E  +     +TQKEE  M+ ++                D    FD F+KEND+KR+RAL K   E +L    ++    LQ E     +    LL++ +   KY+++L +V+E    ++ +V E     +I R+NTL +T N++L  D+     ENQ   +     L   +E+   Q+L+ N +++++    E+   +  Q+E R  QI  T        G IR   LN          I+K+   K + S+   + E +LEKIQ          E ++ I KD       P  P    TN
Sbjct:   12 AFEDKLLVKMPDREDDFLTPATRLLEKRREMVEVEQALSTQKEEFQMKSESLQQRRAELELKEEKLKDSLFKFDKFLKENDSKRKRALHKAAEERQLAAHKEREALRLQAENTQLMQRKGTLLERQEKNSKYQQYLQRVLER-TDEFQEVQE-----MIDRFNTLMATQNKLLKRDL-----ENQELAEREKARLLHYQEETRSQILELNNQIAQLQGELERVRAVAFQWESRWAQIQNTAAENTLRLGRIRMSTLNL------FQTISKQMRLKTEISV--EDTEAQLEKIQI-------CFEDLSAIYKDLKKAGTTPQTPAVPTTN 314
[Close]
BLAST of DUF4200 domain-containing protein vs. TrEMBL
Match: A0A1I8JJ20 (DUF4200 domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 4.101e-64
Identity = 123/312 (39.42%), Postives = 191/312 (61.22%), Query Frame = 1 
Query:   28 KNVFVTQINEREDENSDRVVRHAIVKESPAHLLKSGINTFQSTLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTEILNFGLIKRAVDNITKKCASKFDESIFENEIEIKLEKIQNYVITRRQIIEKV 963
            +NVFVTQ+N+ E+E+  R V   +V E+ A LL +G+NT Q TLV+KKE EL+ ID+DL  KR E++ +M +  ++K  L  +QQA  D+   F+ FI+EN+AKRRRA+ +YQ E RL    ++  E+LQ  +   +     L  KVQ Y+K+E ++  V++ LPPDYIKVS+N++GGLIMRYNTL  T   L   M++  E+ Q      ++LK DHAR +L  N +L++++E  E  K   E+ EQ        IR     +G+  RA+DN+  +C  + D  +   +   K+EKI+++++   +++  V
Sbjct:  831 RNVFVTQLNQAEEEDLVREV--PVVSETGARLLDTGVNTLQKTLVLKKEAELSKIDSDLLQKRREFQDRMRECESKKIVLAEKQQAMKDRVGKFEKFIQENEAKRRRAIQRYQTESRLRAFKEREEEELQSYLALLRRKQAVLSAKVQAYRKFETYMTSVVDILPPDYIKVSDNVIGGLIMRYNTLEDTFNRLYAQMSSVQEQIQETRDRESRLKADHARDVLSKNSQLAELHEATERQKGTQEQHEQQVSYVIQRIRDRSEAYGMTLRAIDNVATRCLKRHDPHLQSLDWPEKMEKIRDHLMDMEEVVRAV 1140
[Close]
BLAST of DUF4200 domain-containing protein vs. TrEMBL
Match: A0A267H748 (DUF4200 domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig023990g2 PE=4 SV=1)

HSP 1 Score: 218.009 bits (554), Expect = 4.556e-64
Identity = 123/313 (39.30%), Postives = 191/313 (61.02%), Query Frame = 1 
Query:   28 KNVFVTQINEREDENSDRVVRHAIVKESPAHLLKSGINTFQSTLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTEILNFGLIKRAVDNITKKCASKFDESIFENEIEIKLEKIQNYVITRRQIIEKVN 966
            +NVFVTQ+N+ E+E+  R V   +V E+ A LL +G+NT Q TLV+KKE EL+ ID+DL  KR E++ +M +  ++K  L  +QQA  D+   F+ FI+EN+AKRRRA+ +YQ E RL    ++  E+LQ  +   +     L  KVQ Y+K+E ++  V++ LPPDYIKVS+N++GGLIMRYNTL  T   L   M++  E+ Q      ++LK DHAR +L  N +L++++E  E  K   E+ EQ        IR     +G+  RA+DN+  +C  + D  +   +   K+EKI+++++   +++  V 
Sbjct:   29 RNVFVTQLNQAEEEDLVREV--PVVSETGARLLDTGVNTLQKTLVLKKEAELSKIDSDLLQKRREFQDRMRECESKKIVLAEKQQAMKDRVGKFEKFIQENEAKRRRAIQRYQTESRLRAFKEREEEELQSYLALLRRKQAVLSAKVQAYRKFETYMTSVVDILPPDYIKVSDNVIGGLIMRYNTLEDTFNRLYAQMSSVQEQIQETRDRESRLKADHARDVLSKNSQLAELHEATERQKGTQEQHEQQVSYVIQRIRDRSEAYGMTLRAIDNVATRCLKRHDPHLQSLDWPEKMEKIRDHLMDMEEVVRAVQ 339
[Close]
BLAST of DUF4200 domain-containing protein vs. TrEMBL
Match: A0A1I8HVF3 (DUF4200 domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 218.009 bits (554), Expect = 6.023e-64
Identity = 123/313 (39.30%), Postives = 191/313 (61.02%), Query Frame = 1 
Query:   28 KNVFVTQINEREDENSDRVVRHAIVKESPAHLLKSGINTFQSTLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTEILNFGLIKRAVDNITKKCASKFDESIFENEIEIKLEKIQNYVITRRQIIEKVN 966
            +NVFVTQ+N+ E+E+  R V   +V E+ A LL +G+NT Q TLV+KKE EL+ ID+DL  KR E++ +M +  ++K  L  +QQA  D+   F+ FI+EN+AKRRRA+ +YQ E RL    ++  E+LQ  +   +     L  KVQ Y+K+E ++  V++ LPPDYIKVS+N++GGLIMRYNTL  T   L   M++  E+ Q      ++LK DHAR +L  N +L++++E  E  K   E+ EQ        IR     +G+  RA+DN+  +C  + D  +   +   K+EKI+++++   +++  V 
Sbjct:   48 RNVFVTQLNQAEEEDLVREV--PVVSETGARLLDTGVNTLQKTLVLKKEAELSKIDSDLLQKRREFQDRMRECESKKIVLAEKQQAMKDRVGKFEKFIQENEAKRRRAIQRYQTESRLRAFKEREEEELQSYLALLRRKQAVLSAKVQAYRKFETYMTSVVDILPPDYIKVSDNVIGGLIMRYNTLEDTFNRLYAQMSSVQEQIQETRDRESRLKADHARDVLSKNSQLAELHEATERQKGTQEQHEQQVSYVIQRIRDRSEAYGMTLRAIDNVATRCLKRHDPHLQSLDWPEKMEKIRDHLMDMEEVVRAVQ 358
[Close]
BLAST of DUF4200 domain-containing protein vs. TrEMBL
Match: A0A0X3PSM2 (Coiled-coil domain-containing protein 42A OS=Schistocephalus solidus OX=70667 GN=CCD42 PE=4 SV=1)

HSP 1 Score: 196.823 bits (499), Expect = 5.337e-56
Identity = 122/344 (35.47%), Postives = 204/344 (59.30%), Query Frame = 1 
Query:   31 NVFVTQINEREDENSDRVVRHAIVKESPAHLLKSGINTFQS---TLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTEILNFGLIKRAVDNITKKCASKFDESIFENEIEIKLEKIQNYVITRRQIIEKVNHIKDKPANNLPPNQPKTIQTNKVKTMPKT 1053
            N F TQ +E   E  D      +  E  A   K   +  +S     +++K+ +L   D  L  KR  +++K++    ++  L  RQ    DQ K F+ F+++N+AKR RAL KYQ E+ L ++  +   DL+ E+   K+++EK+ KKVQ YK +EE+L++VI+ LPPDYIK+++N+LGGLIMRYNTL+ TN  L+ +M  KSE+ +     L  L+E+HA  +L  N  L+ +Y K E   + ++R EQ F   H ++RT++  FG + R++DN+  KCA  +  +  +  + +KL +I +Y++T R+II +V  ++ K ++N  P++    +T  V++  +T
Sbjct:    8 NFFSTQQDESASEEEDTRTTKNLATEVLAENDKDTKDITKSIYGGALLRKQIKLAEADRLLLEKRRYFQRKITASTARRTNLTNRQLELRDQMKKFEGFVRDNEAKRVRALSKYQTEMYLCKVRTREEADLKTELTRVKQVYEKMKKKVQQYKTFEEYLMQVIDMLPPDYIKIADNVLGGLIMRYNTLYETNISLLKEMNEKSEQIREAHAKLVSLREEHASHILTLNSELAALYTKRERRNESFQRHEQRFSNTHRELRTQLSLFGTVIRSIDNLVGKCAWPYTAAPGDLTLSLKLNRIMDYILTTRKIIAEVEQMRTKNSSN--PDKYGKSETRSVQSTYRT 349
[Close]
BLAST of DUF4200 domain-containing protein vs. TrEMBL
Match: K1QVE0 (Coiled-coil domain-containing protein 42-like protein OS=Crassostrea gigas OX=29159 GN=CGI_10022076 PE=4 SV=1)

HSP 1 Score: 195.667 bits (496), Expect = 5.885e-55
Identity = 119/315 (37.78%), Postives = 192/315 (60.95%), Query Frame = 1 
Query:   13 IDEIPKNVFVTQINEREDENSDRVVRHAIVKESPAHLLKSGINTFQSTLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTEILNFGLIKRAVDNITKKCASKFDESIFENEIEIKLEKIQNYVITRRQIIE 957
            +D+  KN+FVTQ++ER+DE+ D V    +VKE+   LL++GINTFQ TL++KKE E+  +D +L   R  + +KM +   +K  +  ++Q   ++   FD FIK+NDAKRRRA+ KYQ EV L E  +   E L  ++   K+    L +KV  YK++EE+LLKVI+ +P DYI+  + I  GL+MR+ TL  +N+ L+ ++    +E +   + L  +K DH ++ +  N  L+K+  + +  +D  ++ EQ F +N GD+R      G+I  A+DNIT+KC  + D S+ +  +E KL+KI  Y+  R  + +
Sbjct:   69 LDDQKKNIFVTQLHERDDEDHD-VTSFPVVKETGGQLLETGINTFQKTLLLKKEVEVAKVDAELEKCRQRFRQKMEELQQRKLNVQKKRQMMNNKVAKFDKFIKDNDAKRRRAIQKYQIEVLLKEQKQTEYEQLCEQLAALKKRKSYLERKVNQYKRFEEYLLKVIDIMPEDYIQDDDKI-KGLMMRHKTLSESNKDLVDNLVHMGDEIEHLKKKLDDMKTDHDKRKVSINSHLAKLQNQQDKRQDSNKQQEQQFASNQGDMRKRRTELGVILMAIDNITEKCLKRLDSSMEDMTLEDKLQKIGAYLQEREDVAQ 381
[Close]
BLAST of DUF4200 domain-containing protein vs. Ensembl Cavefish
Match: si:ch1073-416d2.4 (si:ch1073-416d2.4 [Source:ZFIN;Acc:ZDB-GENE-131121-564])

HSP 1 Score: 96.6709 bits (239), Expect = 4.268e-22
Identity = 75/247 (30.36%), Postives = 142/247 (57.49%), Query Frame = 1 
Query:  121 LLKSGINTFQSTLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTEILNFGLIKRAVDNITKKC 861
            +L +G+NT Q TL++KK+ +++ ++T+L  K+ E+  +M     ++ ELL  +    D+   F+ F++EN+ KRRRAL KYQ E +  E  +K   +L  E     ++H+ L ++V+ YK YEE+L K+++ LP      S+++   +I R+ TL  T + L+  +T  SEE ++  + L  LK++H    L  N  +S++  +++ +K++ ++ E   + + G  R +    G +  AV N+ ++C
Sbjct:   24 ILDTGVNTLQRTLLLKKQLKVDEVNTELKQKQLEFRARMQILEKRRAELLQTK----DRAVKFEKFVEENEVKRRRALKKYQVERKQNEQKEKEKSEL-CEHFDQLQIHQYLKQRVEKYKMYEEYLRKILDLLPEYLEYGSDSLAMPIIRRHETLSITQQTLVQHLTGLSEELEQGQRNLDSLKQEHNTSKLMINKEMSELQTQWDQIKEKNKQLEMTLQIHQGQNRDQAEEIGSLMIAVKNLGQQC 265
[Close]
BLAST of DUF4200 domain-containing protein vs. Ensembl Cavefish
Match: cfap73 (coiled-coil domain-containing protein 42 homolog [Source:NCBI gene;Acc:103032501])

HSP 1 Score: 63.1586 bits (152), Expect = 9.883e-11
Identity = 74/284 (26.06%), Postives = 131/284 (46.13%), Query Frame = 1 
Query:  112 PAHLLKSGINTFQSTLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTEILNFGLIKRAVDNI---TKKCASKFDESIFENEIEIKLEKIQNYVITRRQII 954
            P   L++G     +T ++ K  E+  +D  L N++ E+E K      +++EL  +++   D    F+ ++K+NDAK+ R L K + E  L+   +K ++ LQ  I       EK+ K+V   + Y  FL  V++       K  E+I G  I R+ TL    E L+   +    E +R    L +   + +  LL  N  LS++  + +T+  Q  R E  +         E L  G IK    N+   T +  S  +    ++ ++ +L++IQ Y+  R  I+
Sbjct:   23 PEEDLRTG-----TTRILDKRREIKYMDDVLRNQKEEFEAKKKSLEQRRDELRKKEEKLKDSLLNFNKYLKDNDAKQARGLKKAEAEKALVREREKEVQQLQRHIAALLTKKEKMQKRVSQKRIYWSFLDSVLK-----SSKKFEDI-GKFIGRFETLVCMREQLLKRQSTVESEREREAVQLRRYVSERSSALLHYNNTLSQLQTELDTILSQALRWESTWNHIQATAAKETLLLGQIKIVTLNLYHMTGRVTSGAEGFNVDDTLD-QLDRIQLYIQKRADIV 294
[Close]
BLAST of DUF4200 domain-containing protein vs. Ensembl Cavefish
Match: cfap73 (coiled-coil domain-containing protein 42 homolog [Source:NCBI gene;Acc:103032501])

HSP 1 Score: 60.4622 bits (145), Expect = 5.384e-10
Identity = 65/240 (27.08%), Postives = 110/240 (45.83%), Query Frame = 1 
Query:  112 PAHLLKSGINTFQSTLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTEILNFGLIK 831
            P   L++G     +T ++ K  E+  +D  L N++ E+E K      +++EL  +++   D    F+ ++K+NDAK+ R L K + E  L+   +K ++ LQ  I       EK+ K+V   + Y  FL  V++       K  E+I G  I R+ TL    E L+   +    E +R    L +   + +  LL  N  LS++  + +T+  Q  R E  +         E L  G IK
Sbjct:   23 PEEDLRTG-----TTRILDKRREIKYMDDVLRNQKEEFEAKKKSLEQRRDELRKKEEKLKDSLLNFNKYLKDNDAKQARGLKKAEAEKALVREREKEVQQLQRHIAALLTKKEKMQKRVSQKRIYWSFLDSVLK-----SSKKFEDI-GKFIGRFETLVCMREQLLKRQSTVESEREREAVQLRRYVSERSSALLHYNNTLSQLQTELDTILSQALRWESTWNHIQATAAKETLLLGQIK 251
[Close]
BLAST of DUF4200 domain-containing protein vs. Ensembl Cavefish
Match: cfap73 (coiled-coil domain-containing protein 42 homolog [Source:NCBI gene;Acc:103032501])

HSP 1 Score: 56.225 bits (134), Expect = 1.195e-8
Identity = 60/220 (27.27%), Postives = 104/220 (47.27%), Query Frame = 1 
Query:  112 PAHLLKSGINTFQSTLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQ 771
            P   L++G     +T ++ K  E+  +D  L N++ E+E K      +++EL  +++   D    F+ ++K+NDAK+ R L K + E  L+   +K ++ LQ  I       EK+ K+V   + Y  FL  V++       K  E+I G  I R+ TL    E L+   +    E +R    L +   + +  LL  N  LS++  + +T+  Q  R  Q
Sbjct:   23 PEEDLRTG-----TTRILDKRREIKYMDDVLRNQKEEFEAKKKSLEQRRDELRKKEEKLKDSLLNFNKYLKDNDAKQARGLKKAEAEKALVREREKEVQQLQRHIAALLTKKEKMQKRVSQKRIYWSFLDSVLK-----SSKKFEDI-GKFIGRFETLVCMREQLLKRQSTVESEREREAVQLRRYVSERSSALLHYNNTLSQLQTELDTILSQALRWVQ 231
[Close]
BLAST of DUF4200 domain-containing protein vs. Ensembl Nematostella
Match: EDO38155 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDR1])

HSP 1 Score: 90.8929 bits (224), Expect = 1.737e-20
Identity = 61/182 (33.52%), Postives = 104/182 (57.14%), Query Frame = 1 
Query:  292 DQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTE----ILNFGL 825
            D+   F+ FIKEN+AKRRRA+ KYQ E++L +   K  E L  E++  K     L  K+  Y  YE++LLKV+++LP DY++ S+++L G++MR+ TL +TN  LM  ++ K++  ++  Q L  +  +H + L+  N  L+ + +  E    +    +Q+   N   +R +    +L  GL
Sbjct:   44 DRVGKFEKFIKENEAKRRRAVQKYQTELKLKDQKSKEEEILAAELQQLKARKHSLEAKIAKYSVYEKYLLKVLDFLPEDYLEASDSMLMGIMMRFRTLSATNHTLMQMLSDKADGVEQSQQKLLDMNNNHTQHLVLINSELAHLQQILEETSQKNAELDQLLINNKTTVRHQTSGKLLETGL 225
[Close]
BLAST of DUF4200 domain-containing protein vs. Ensembl Nematostella
Match: EDO39885 (Coiled-coil domain-containing protein 42 homolog [Source:UniProtKB/Swiss-Prot;Acc:A7S8T5])

HSP 1 Score: 55.0694 bits (131), Expect = 1.430e-8
Identity = 62/261 (23.75%), Postives = 126/261 (48.28%), Query Frame = 1 
Query:  151 STLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTEILNFGLIKRAVDNITKKCASKFDESIFENEIEIKLEKIQNYV 933
            +T +++K  E++ ++  L  ++ E++ KM     ++EEL  ++    +    FD F+KEND+KR RAL K Q E  +       I  L+ +     +  +K+  +++ Y  Y+++L KV+E    ++ ++ E     +I RY+TL +T++ L+       E+ ++    L K  E+   ++L  N +L+ +  K E  +    + E  +         + L  G IK A  N+         ++   +  + +L+KIQ ++
Sbjct:   32 ATRLLEKRREMSEVEQALGAQKEEFQMKMESLQQRREELERKEYQLRESLLKFDKFLKENDSKRARALKKAQEERDMRRAKDCEIARLKEDTSGLMKGRDKVQHRLEKYIIYQQYLEKVLEN-AEEFQEIRE-----IIARYDTLTATHQDLLERELKNQEKYEKEKARLIKFTEEKNNEILNYNNQLANLQTKLEKTQSVAVKWESQWTHIKNTAAKKTLLLGRIKMATHNLFMLVNRHLGQTGMVDMTDKQLDKIQVFI 286
[Close]
BLAST of DUF4200 domain-containing protein vs. Planmine SMEST
Match: SMESG000028991.1 (SMESG000028991.1)

HSP 1 Score: 676.011 bits (1743), Expect = 0.000e+0
Identity = 353/355 (99.44%), Postives = 353/355 (99.44%), Query Frame = 1 
Query:    1 MLNIIDEIPKNVFVTQINEREDENSDRVVRHAIVKESPAHLLKSGINTFQSTLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTEILNFGLIKRAVDNITKKCASKFDESIFENEIEIKLEKIQNYVITRRQIIEKVNHIKDKPANNLPPNQPKTIQTNKVKTMPKTYSKN 1065
            MLNIIDEIPKNVFVTQINEREDENSDRVVRHAIVKESPAHLLKSGINTFQSTLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQIEIKTTKELHEKLLKKVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYER EQIFKTNHGDIRTEILNFGLIKRAVDNITKKCASKFDESIFENEIEIKLEKIQNYVITRRQIIEKVNHIKDKPANNLPPNQPKTIQTNKVKTMPK YSKN
Sbjct:    1 MLNIIDEIPKNVFVTQINEREDENSDRVVRHAIVKESPAHLLKSGINTFQSTLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQIEIKTTKELHEKLLKKVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERAEQIFKTNHGDIRTEILNFGLIKRAVDNITKKCASKFDESIFENEIEIKLEKIQNYVITRRQIIEKVNHIKDKPANNLPPNQPKTIQTNKVKTMPKIYSKN 355
[Close]
BLAST of DUF4200 domain-containing protein vs. Planmine SMEST
Match: SMESG000028991.1 (SMESG000028991.1)

HSP 1 Score: 659.448 bits (1700), Expect = 0.000e+0
Identity = 343/347 (98.85%), Postives = 345/347 (99.42%), Query Frame = 1 
Query:    1 MLNIIDEIPKNVFVTQINEREDENSDRVVRHAIVKESPAHLLKSGINTFQSTLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTEILNFGLIKRAVDNITKKCASKFDESIFENEIEIKLEKIQNYVITRRQIIEKVNHIKDKPANNLPPNQPKTIQTNKVKT 1041
            MLNIIDEIPKNVFVTQINEREDENSDRVVRHAIVKESPAHLLKSGINTFQSTLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQIEIKTTKELHEKLLKKVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYER EQIFKTNHGDIRTEILNFGLIKRAVDNITKKCASKFDESIFENEIEIKLEKIQNYVITRRQIIEKVNHIKDKPANNLPPNQPKTIQTNK++ 
Sbjct:    1 MLNIIDEIPKNVFVTQINEREDENSDRVVRHAIVKESPAHLLKSGINTFQSTLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQIEIKTTKELHEKLLKKVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERAEQIFKTNHGDIRTEILNFGLIKRAVDNITKKCASKFDESIFENEIEIKLEKIQNYVITRRQIIEKVNHIKDKPANNLPPNQPKTIQTNKLRA 347
[Close]
BLAST of DUF4200 domain-containing protein vs. Planmine SMEST
Match: SMESG000010261.1 (SMESG000010261.1)

HSP 1 Score: 71.2478 bits (173), Expect = 1.524e-13
Identity = 72/282 (25.53%), Postives = 142/282 (50.35%), Query Frame = 1 
Query:  169 KEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQ-TKTFDSFIKENDAKRRRALIKYQNEVR----LIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSEN-ILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTEILNFGLIKRAVDNITKKCASKFDESIFENEIEIKLEKIQNYVITRRQIIEKVNHIKDKPANNL 996
            KE +LN +   +  K NE + K+++    KE +  + Q+F+D+  +   +F   N  K+ +AL KY+   +    L++  KK   D ++  +  + +   + K + H    + F+ + I+ LP DY+K  ++  + GL+ R+ +LF  NE+L  ++  K+   +    + T+LK       +Q   ++ K+Y   +++  + E  E++++  + + R +I  FG +  ++DN+  KC S +     EN   +K     +Y++    I EKV  IK+   NNL
Sbjct:   39 KENKLNLLKELILKKYNEMQVKLTQTLKLKESM-TQCQSFIDEKVEKLQNFTLLNKYKKLKALEKYKQVKKSRHFLMKKWKKVHADTEVLEQRHRRIRTLIEKMLSH----KNFIQRTIDLLPKDYVKTRKDQSIEGLMGRFKSLFYVNEVLFANIGVKNNNLKILKGVETELKSSRELCTIQALAKIKKLYMTKDSVTSRVEEKERLYRKKYSETRKKIKTFGRLLLSLDNLANKCTSFYKLFGIENWTFVK-----SYLLKTMTIDEKVKKIKEYIDNNL 310
[Close]
BLAST of DUF4200 domain-containing protein vs. Planmine SMEST
Match: SMESG000013593.1 (SMESG000013593.1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.634e-11
Identity = 59/242 (24.38%), Postives = 123/242 (50.83%), Query Frame = 1 
Query:  151 STLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQTKTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIKVSEN-ILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTEILNFGLIKRAVDNITKKCASKF 873
            S   + ++ +L+++  +L+ + N ++K+M     +K ++  R Q    QT  F++F  EN  KRRRA  K  NE       ++T+  L+ E +  +   +++   +++ K Y +F+  V+E LP DYIK  ++  +   + R+N+LF  NE+L  +M   +++ +   + + +++ ++   L+  +    KM  + ++ K         +      IR +I   G+I  ++DNI  KC  ++
Sbjct:   25 SVECLNQQEKLSNVKDNLFERFNTFQKQMETIEIEKLKIEKRNQIQEKQTVNFENFSIENAYKRRRAFHKIMNEKETEIKMQRTLITLEKEYEKLQNRKDRISLLIENMKPYHQFIKDVLELLPSDYIKTHKDQTVEAFMSRFNSLFFINELLYKEMLVSNDKMRALKEQMNEMQTNNFNYLIHISTIFLKMIAEKDSSKLTLYANTLKYFDYFRVIRRKIHKNGVIFISIDNILNKCIFQY 266
[Close]
BLAST of DUF4200 domain-containing protein vs. Planmine SMEST
Match: SMESG000009547.1 (SMESG000009547.1)

HSP 1 Score: 51.9878 bits (123), Expect = 3.304e-7
Identity = 55/210 (26.19%), Postives = 108/210 (51.43%), Query Frame = 1 
Query:  316 FIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQXXXXXXXXXXXXXXXXVQHYKKYEEFLLKVIEYLPPDYIK-VSENILGGLIMRYNTLFSTNEMLMVDMTAKSEENQRFVQLL----TKLKEDHARQLLQNNYRLSKMYEKYETLKDQYERGEQIFKTNHGDIRTEILNFGLIKRAVDNITKKCASKFDESIFENEIEIKL-EKIQN 927
            FI+EN+ +  + +   ++++R+  + +K + ++  EI+         +   +  K YE FL+  ++ LP DY+K + EN +  LI R  +LF  N++L  D+   S   ++F++ L     KL    A Q L    +L+    +Y+ +  Q +     +  ++  +R ++  FG++ ++++NIT K A +  E  F  E+E+ L  KI N
Sbjct:  106 FIEENNYRYHKTVESIKHDIRMTRIKRKQLLEIDKEIEGLSNKKNYYIPANKQLKNYENFLIDALQILPEDYVKGLKENNMNALIRRCRSLFYINKILNEDLNRSSALIRKFIKDLDFVYQKLSSLQAHQSLIFP-KLNNQLLEYQNVLSQKQSK---YSDSYISLRQKLHKFGIMLQSINNITNK-AFQILEKGFYCELELNLIPKIMN 310
The following BLAST results are available for this feature:
BLAST of DUF4200 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF4200 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF4200 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF4200 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
si:ch1073-416d2.46.396e-2729.07si:ch1073-416d2.4 [Source:ZFIN;Acc:ZDB-GENE-131121... [more]
si:ch1073-416d2.48.980e-2035.09si:ch1073-416d2.4 [Source:ZFIN;Acc:ZDB-GENE-131121... [more]
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BLAST of DUF4200 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
klhl136.420e-727.36kelch like family member 13 [Source:Xenbase;Acc:XB... [more]
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BLAST of DUF4200 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Ccdc422.539e-725.00coiled-coil domain containing 42 [Source:MGI Symbo... [more]
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BLAST of DUF4200 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|A9UQN0|CCD42_MONBE4.698e-823.92Coiled-coil domain-containing protein 42 homolog O... [more]
sp|A7S8T5|CCD42_NEMVE3.489e-723.75Coiled-coil domain-containing protein 42 homolog O... [more]
sp|A6QQM8|CCD42_BOVIN8.863e-724.39Coiled-coil domain-containing protein 42 OS=Bos ta... [more]
sp|Q5SV66|CCD42_MOUSE1.776e-625.00Coiled-coil domain-containing protein 42 OS=Mus mu... [more]
sp|B0BMJ2|CC42M_XENTR3.401e-627.36Coiled-coil domain-containing protein 42 like-2 OS... [more]
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BLAST of DUF4200 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8JJ204.101e-6439.42DUF4200 domain-containing protein OS=Macrostomum l... [more]
A0A267H7484.556e-6439.30DUF4200 domain-containing protein (Fragment) OS=Ma... [more]
A0A1I8HVF36.023e-6439.30DUF4200 domain-containing protein OS=Macrostomum l... [more]
A0A0X3PSM25.337e-5635.47Coiled-coil domain-containing protein 42A OS=Schis... [more]
K1QVE05.885e-5537.78Coiled-coil domain-containing protein 42-like prot... [more]
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BLAST of DUF4200 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
si:ch1073-416d2.44.268e-2230.36si:ch1073-416d2.4 [Source:ZFIN;Acc:ZDB-GENE-131121... [more]
cfap739.883e-1126.06coiled-coil domain-containing protein 42 homolog [... [more]
cfap735.384e-1027.08coiled-coil domain-containing protein 42 homolog [... [more]
cfap731.195e-827.27coiled-coil domain-containing protein 42 homolog [... [more]
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BLAST of DUF4200 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF4200 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF4200 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO381551.737e-2033.52Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO398851.430e-823.75Coiled-coil domain-containing protein 42 homolog ... [more]
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BLAST of DUF4200 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF4200 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000028991.10.000e+099.44SMESG000028991.1[more]
SMESG000028991.10.000e+098.85SMESG000028991.1[more]
SMESG000010261.11.524e-1325.53SMESG000010261.1[more]
SMESG000013593.11.634e-1124.38SMESG000013593.1[more]
SMESG000009547.13.304e-726.19SMESG000009547.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012638 ID=SMED30012638|Name=DUF4200 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1125bp
ATGTTGAACATAATTGATGAAATCCCAAAAAATGTTTTTGTTACACAAAT
AAATGAAAGAGAGGATGAAAATTCTGATCGTGTAGTTAGACATGCAATTG
TCAAAGAAAGTCCTGCTCACTTGTTAAAGTCAGGCATAAATACTTTTCAA
AGTACTCTGGTTATTAAAAAAGAAGGTGAATTAAATTCAATTGATACGGA
TTTATACAACAAAAGAAATGAATATGAAAAAAAAATGTCGAAATATAATA
CTCAAAAAGAAGAGTTGCTTATGAGGCAGCAAGCGTTTCTTGATCAAACC
AAAACTTTTGACTCATTTATTAAAGAAAATGATGCAAAACGACGACGCGC
GTTGATAAAGTATCAAAACGAAGTTCGATTAATTGAGTTGCATAAAAAAA
CCATTGAAGATCTTCAAATAGAAATAAAAACTACTAAAGAACTGCATGAA
AAACTTTTGAAAAAAGTTCAACACTACAAAAAGTACGAAGAATTTTTATT
GAAAGTCATCGAATACTTGCCACCAGACTACATAAAAGTTTCGGAAAATA
TTTTGGGTGGATTAATAATGCGATATAATACTTTATTTTCCACAAATGAA
ATGCTAATGGTCGACATGACAGCGAAATCTGAAGAGAATCAGCGATTTGT
GCAACTGCTAACAAAACTGAAAGAAGATCATGCACGGCAGTTGTTACAAA
ATAATTACAGACTCTCGAAAATGTATGAAAAATATGAAACATTGAAAGAT
CAGTACGAAAGAGGAGAACAGATCTTTAAAACGAATCACGGAGATATTCG
CACTGAGATTTTAAATTTTGGCTTGATTAAGAGGGCGGTTGATAATATCA
CAAAAAAATGTGCGAGTAAATTTGATGAATCAATATTTGAAAATGAAATA
GAGATCAAACTTGAAAAAATACAAAATTATGTAATTACTCGCAGACAGAT
CATTGAAAAAGTAAATCACATAAAAGACAAACCAGCGAACAACCTCCCTC
CCAATCAACCGAAAACTATTCAAACTAACAAAGTAAAAACAATGCCAAAG
ACTTATTCCAAAAATTAATTGTGGTGAAACATTTAGATAATAAAATGAAT
GAAAATAAAATTTATTATTGCTCGG
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protein sequence of SMED30012638-orf-1

>SMED30012638-orf-1 ID=SMED30012638-orf-1|Name=SMED30012638-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=356bp
MLNIIDEIPKNVFVTQINEREDENSDRVVRHAIVKESPAHLLKSGINTFQ
STLVIKKEGELNSIDTDLYNKRNEYEKKMSKYNTQKEELLMRQQAFLDQT
KTFDSFIKENDAKRRRALIKYQNEVRLIELHKKTIEDLQIEIKTTKELHE
KLLKKVQHYKKYEEFLLKVIEYLPPDYIKVSENILGGLIMRYNTLFSTNE
MLMVDMTAKSEENQRFVQLLTKLKEDHARQLLQNNYRLSKMYEKYETLKD
QYERGEQIFKTNHGDIRTEILNFGLIKRAVDNITKKCASKFDESIFENEI
EIKLEKIQNYVITRRQIIEKVNHIKDKPANNLPPNQPKTIQTNKVKTMPK
TYSKN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR025252DUF4200
Vocabulary: biological process
TermDefinition
GO:0007286spermatid development
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 72..92
NoneNo IPR availableCOILSCoilCoilcoord: 238..258
NoneNo IPR availableCOILSCoilCoilcoord: 128..155
NoneNo IPR availablePANTHERPTHR21683:SF10coord: 10..326
NoneNo IPR availablePANTHERPTHR21683UNCHARACTERIZEDcoord: 10..326
IPR025252Domain of unknown function DUF4200PFAMPF13863DUF4200coord: 55..171
e-value: 1.3E-19
score: 70.8