SMED30012619

Overview
NameSMED30012619
Smed IDSMED30012619
Length (bp)1347
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30012619 (SMED30012619) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30012619 (SMED30012619) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30012619 (SMED30012619) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30012619 (SMED30012619) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012619

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30012619SMESG000006885.1 Contig485uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30012619SMESG000006885.1 Contig485newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30012619SMESG000005010.1 Contig485uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30012619SMESG000005010.1 Contig485newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
X1 cellSMED30012619SMESG000006885.1 SmedASXL_011138SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30012619SMESG000006885.1 SmedASXL_011138SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
epidermisSMED30012619SMESG000006885.1 dd_Smed_v4_7366_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30012619SMESG000006885.1 dd_Smed_v4_7366_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30012619 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002508:22587..25491 -5701
v31.002372:82759..85597 +5688
Homology
[Close]
BLAST of SMED30012619 vs. Ensembl Human
Match: MATR3 (matrin 3 [Source:HGNC Symbol;Acc:HGNC:6912])

HSP 1 Score: 49.6766 bits (117), Expect = 4.463e-6
Identity = 18/55 (32.73%), Postives = 34/55 (61.82%), Query Frame = 1 
Query:  640 GQEMLIPVNGVFCALCEIFFANKDVAEKDHCSTQAHCEAYEKLLKRVQESKSDRR 804
            G + +IP  G +C LC +F+ N++VA+  HCS+  H +  +K L ++ E +  ++
Sbjct:  227 GIDYVIPKTGFYCKLCSLFYTNEEVAKNTHCSSLPHYQKLKKFLNKLAEERRQKK 281
[Close]
BLAST of SMED30012619 vs. Ensembl Human
Match: MATR3 (matrin 3 [Source:NCBI gene;Acc:9782])

HSP 1 Score: 50.8322 bits (120), Expect = 4.646e-6
Identity = 18/55 (32.73%), Postives = 34/55 (61.82%), Query Frame = 1 
Query:  640 GQEMLIPVNGVFCALCEIFFANKDVAEKDHCSTQAHCEAYEKLLKRVQESKSDRR 804
            G + +IP  G +C LC +F+ N++VA+  HCS+  H +  +K L ++ E +  ++
Sbjct:  791 GIDYVIPKTGFYCKLCSLFYTNEEVAKNTHCSSLPHYQKLKKFLNKLAEERRQKK 845
[Close]
BLAST of SMED30012619 vs. Ensembl Human
Match: MATR3 (matrin 3 [Source:NCBI gene;Acc:9782])

HSP 1 Score: 50.8322 bits (120), Expect = 4.646e-6
Identity = 18/55 (32.73%), Postives = 34/55 (61.82%), Query Frame = 1 
Query:  640 GQEMLIPVNGVFCALCEIFFANKDVAEKDHCSTQAHCEAYEKLLKRVQESKSDRR 804
            G + +IP  G +C LC +F+ N++VA+  HCS+  H +  +K L ++ E +  ++
Sbjct:  791 GIDYVIPKTGFYCKLCSLFYTNEEVAKNTHCSSLPHYQKLKKFLNKLAEERRQKK 845
[Close]
BLAST of SMED30012619 vs. Ensembl Human
Match: MATR3 (matrin 3 [Source:HGNC Symbol;Acc:HGNC:6912])

HSP 1 Score: 50.8322 bits (120), Expect = 4.646e-6
Identity = 18/55 (32.73%), Postives = 34/55 (61.82%), Query Frame = 1 
Query:  640 GQEMLIPVNGVFCALCEIFFANKDVAEKDHCSTQAHCEAYEKLLKRVQESKSDRR 804
            G + +IP  G +C LC +F+ N++VA+  HCS+  H +  +K L ++ E +  ++
Sbjct:  791 GIDYVIPKTGFYCKLCSLFYTNEEVAKNTHCSSLPHYQKLKKFLNKLAEERRQKK 845
[Close]
BLAST of SMED30012619 vs. Ensembl Human
Match: MATR3 (matrin 3 [Source:HGNC Symbol;Acc:HGNC:6912])

HSP 1 Score: 50.8322 bits (120), Expect = 4.646e-6
Identity = 18/55 (32.73%), Postives = 34/55 (61.82%), Query Frame = 1 
Query:  640 GQEMLIPVNGVFCALCEIFFANKDVAEKDHCSTQAHCEAYEKLLKRVQESKSDRR 804
            G + +IP  G +C LC +F+ N++VA+  HCS+  H +  +K L ++ E +  ++
Sbjct:  791 GIDYVIPKTGFYCKLCSLFYTNEEVAKNTHCSSLPHYQKLKKFLNKLAEERRQKK 845
[Close]
BLAST of SMED30012619 vs. Ensembl Zebrafish
Match: CR352328.1 (si:ch211-212k18.4 [Source:NCBI gene;Acc:563749])

HSP 1 Score: 50.0618 bits (118), Expect = 5.503e-6
Identity = 19/42 (45.24%), Postives = 27/42 (64.29%), Query Frame = 1 
Query:  640 GQEMLIPVNGVFCALCEIFFANKDVAEKDHCSTQAHCEAYEK 765
            G + +IP  G FC LC +F+ N+D A+K HCS+  H +  EK
Sbjct: 2120 GIDFVIPRTGFFCKLCSLFYVNEDTAKKSHCSSLKHYQNMEK 2161
[Close]
BLAST of SMED30012619 vs. Ensembl Zebrafish
Match: si:ch211-212k18.4 (si:ch211-212k18.4 [Source:ZFIN;Acc:ZDB-GENE-141216-265])

HSP 1 Score: 50.0618 bits (118), Expect = 5.505e-6
Identity = 19/42 (45.24%), Postives = 27/42 (64.29%), Query Frame = 1 
Query:  640 GQEMLIPVNGVFCALCEIFFANKDVAEKDHCSTQAHCEAYEK 765
            G + +IP  G FC LC +F+ N+D A+K HCS+  H +  EK
Sbjct: 2127 GIDFVIPRTGFFCKLCSLFYVNEDTAKKSHCSSLKHYQNMEK 2168
[Close]
BLAST of SMED30012619 vs. Ensembl Zebrafish
Match: si:ch211-212k18.4 (si:ch211-212k18.4 [Source:ZFIN;Acc:ZDB-GENE-141216-265])

HSP 1 Score: 50.0618 bits (118), Expect = 5.505e-6
Identity = 19/42 (45.24%), Postives = 27/42 (64.29%), Query Frame = 1 
Query:  640 GQEMLIPVNGVFCALCEIFFANKDVAEKDHCSTQAHCEAYEK 765
            G + +IP  G FC LC +F+ N+D A+K HCS+  H +  EK
Sbjct: 2127 GIDFVIPRTGFFCKLCSLFYVNEDTAKKSHCSSLKHYQNMEK 2168
[Close]
BLAST of SMED30012619 vs. Ensembl Mouse
Match: Matr3 (matrin 3 [Source:MGI Symbol;Acc:MGI:1298379])

HSP 1 Score: 50.8322 bits (120), Expect = 3.134e-6
Identity = 18/55 (32.73%), Postives = 34/55 (61.82%), Query Frame = 1 
Query:  640 GQEMLIPVNGVFCALCEIFFANKDVAEKDHCSTQAHCEAYEKLLKRVQESKSDRR 804
            G + +IP  G +C LC +F+ N++VA+  HCS+  H +  +K L ++ E +  ++
Sbjct:  790 GIDYVIPKTGFYCKLCSLFYTNEEVAKNTHCSSLPHYQKLKKFLNKLAEERRQKK 844
[Close]
BLAST of SMED30012619 vs. Ensembl Mouse
Match: Matr3 (matrin 3 [Source:MGI Symbol;Acc:MGI:1298379])

HSP 1 Score: 50.8322 bits (120), Expect = 3.134e-6
Identity = 18/55 (32.73%), Postives = 34/55 (61.82%), Query Frame = 1 
Query:  640 GQEMLIPVNGVFCALCEIFFANKDVAEKDHCSTQAHCEAYEKLLKRVQESKSDRR 804
            G + +IP  G +C LC +F+ N++VA+  HCS+  H +  +K L ++ E +  ++
Sbjct:  790 GIDYVIPKTGFYCKLCSLFYTNEEVAKNTHCSSLPHYQKLKKFLNKLAEERRQKK 844
[Close]
BLAST of SMED30012619 vs. Ensembl Mouse
Match: Matr3 (matrin 3 [Source:MGI Symbol;Acc:MGI:1298379])

HSP 1 Score: 50.8322 bits (120), Expect = 3.134e-6
Identity = 18/55 (32.73%), Postives = 34/55 (61.82%), Query Frame = 1 
Query:  640 GQEMLIPVNGVFCALCEIFFANKDVAEKDHCSTQAHCEAYEKLLKRVQESKSDRR 804
            G + +IP  G +C LC +F+ N++VA+  HCS+  H +  +K L ++ E +  ++
Sbjct:  790 GIDYVIPKTGFYCKLCSLFYTNEEVAKNTHCSSLPHYQKLKKFLNKLAEERRQKK 844
[Close]
BLAST of SMED30012619 vs. Ensembl Mouse
Match: Matr3 (matrin 3 [Source:MGI Symbol;Acc:MGI:1298379])

HSP 1 Score: 50.447 bits (119), Expect = 3.140e-6
Identity = 18/55 (32.73%), Postives = 34/55 (61.82%), Query Frame = 1 
Query:  640 GQEMLIPVNGVFCALCEIFFANKDVAEKDHCSTQAHCEAYEKLLKRVQESKSDRR 804
            G + +IP  G +C LC +F+ N++VA+  HCS+  H +  +K L ++ E +  ++
Sbjct:  502 GIDYVIPKTGFYCKLCSLFYTNEEVAKNTHCSSLPHYQKLKKFLNKLAEERRQKK 556
[Close]
BLAST of SMED30012619 vs. Ensembl Mouse
Match: Matr3 (matrin 3 [Source:MGI Symbol;Acc:MGI:1298379])

HSP 1 Score: 50.447 bits (119), Expect = 3.234e-6
Identity = 18/55 (32.73%), Postives = 34/55 (61.82%), Query Frame = 1 
Query:  640 GQEMLIPVNGVFCALCEIFFANKDVAEKDHCSTQAHCEAYEKLLKRVQESKSDRR 804
            G + +IP  G +C LC +F+ N++VA+  HCS+  H +  +K L ++ E +  ++
Sbjct:  452 GIDYVIPKTGFYCKLCSLFYTNEEVAKNTHCSSLPHYQKLKKFLNKLAEERRQKK 506
[Close]
BLAST of SMED30012619 vs. Ensembl Medaka
Match: ENSORLT00000044190.1 (zinc finger protein 638 [Source:NCBI gene;Acc:101174778])

HSP 1 Score: 49.6766 bits (117), Expect = 7.082e-6
Identity = 18/48 (37.50%), Postives = 32/48 (66.67%), Query Frame = 1 
Query:  640 GQEMLIPVNGVFCALCEIFFANKDVAEKDHCSTQAHCEAYEKLLKRVQ 783
            GQE ++P +G FC +C +F+  +  A++ HCS+Q H E  +K  +++Q
Sbjct: 2433 GQEHVVPKSGFFCNICSVFYLTEKAAKEVHCSSQRHYENLQKHNRKLQ 2480
[Close]
BLAST of SMED30012619 vs. Ensembl Medaka
Match: ENSORLT00000033229.1 (zinc finger protein 638 [Source:NCBI gene;Acc:101174778])

HSP 1 Score: 49.6766 bits (117), Expect = 7.085e-6
Identity = 18/48 (37.50%), Postives = 32/48 (66.67%), Query Frame = 1 
Query:  640 GQEMLIPVNGVFCALCEIFFANKDVAEKDHCSTQAHCEAYEKLLKRVQ 783
            GQE ++P +G FC +C +F+  +  A++ HCS+Q H E  +K  +++Q
Sbjct: 2433 GQEHVVPKSGFFCNICSVFYLTEKAAKEVHCSSQRHYENLQKHNRKLQ 2480
[Close]
BLAST of SMED30012619 vs. Planmine SMEST
Match: SMESG000006885.1 (SMESG000006885.1)

HSP 1 Score: 362.844 bits (930), Expect = 2.960e-140
Identity = 267/301 (88.70%), Postives = 273/301 (90.70%), Query Frame = 1 
Query:   76 VPPPSFSNDISLNSMSYGASIPRFNVWQSSLTPVQNVQQSYPNPQAKKNYCSTCDMWTSEPYNNHQISPAHLAKKIGQRGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKRKFKRSNRRVSCLKFNSFQRTDXXXXXXXXXXXXXXXXXXXXDDHKTDAQNSSXXXXXXXXXXXIKMLNVNRNRGQEMLIPVNGVFCALCEIFFANKDVAEKDHCSTQAHCEAYEKLLKRVQESKSDRRVNEKXXXXXXXXXXXXXXXXXXXXXXXXXXCKRLKLTPAENQDKDFRILSSIPHVVAV 978
            VPPPSFSNDISLNSMSYGASIPRFNVWQSSLTPVQNVQQSYPNPQAKKNYCSTCDMWTSEPYNNHQISPAHLAKKIGQRGNQNYQTSYSNTNSSSSITSNNNLCNNNSNANLNKRKFKRS               + +NRRQSSSNKSISEESAINDDHKTDAQNSSDEKKDDKLKEDIKMLNVNRNRGQEMLIPVNGVFCALCEIFFANKDVAEKDHCSTQAHCEAYEKLLKRVQESKSDRRVNEK S D+    +++    NN  DDDDENCKRLKLTPAENQDKDFRILSSIPHVVAV
Sbjct:    7 VPPPSFSNDISLNSMSYGASIPRFNVWQSSLTPVQNVQQSYPNPQAKKNYCSTCDMWTSEPYNNHQISPAHLAKKIGQRGNQNYQTSYSNTNSSSSITSNNNLCNNNSNANLNKRKFKRS---------------NRRNRRQSSSNKSISEESAINDDHKTDAQNSSDEKKDDKLKEDIKMLNVNRNRGQEMLIPVNGVFCALCEIFFANKDVAEKDHCSTQAHCEAYEKLLKRVQESKSDRRVNEK-SSDDNGNADNSNGDGNNNKDDDDENCKRLKLTPAENQDKDFRILSSIPHVVAV 291

HSP 2 Score: 155.992 bits (393), Expect = 2.960e-140
Identity = 90/94 (95.74%), Postives = 92/94 (97.87%), Query Frame = 2 
Query:  980 RSRRSGIPTRSTTTANYRPAKTARKSIGIPLMISTRQISRWISPMTWRTMSMTLSICRPTRKRQPKNAAXXXXXXXXXXXXNQKSINSEDEHAP 1261
            RSRRSGIPTRSTTTANYRPA+TARKSIGIPLMIST QISRWISPMTWRTMSMTLSICRPTRKRQPKNAALLSL+VTVIVIINQKSINSEDE AP
Sbjct:  292 RSRRSGIPTRSTTTANYRPARTARKSIGIPLMISTLQISRWISPMTWRTMSMTLSICRPTRKRQPKNAALLSLLVTVIVIINQKSINSEDERAP 385
[Close]
BLAST of SMED30012619 vs. Planmine SMEST
Match: SMESG000078442.1 (SMESG000078442.1)

HSP 1 Score: 62.3882 bits (150), Expect = 9.535e-11
Identity = 23/51 (45.10%), Postives = 36/51 (70.59%), Query Frame = 1 
Query:  640 GQEMLIPVNGVFCALCEIFFANKDVAEKDHCSTQAHCEAYEKLLKRVQESK 792
            G+EML+PV G FC++C++FF  +D A ++HC+T+ H E Y KL     +S+
Sbjct:   96 GEEMLLPVQGYFCSICDLFFETRDKAYQEHCTTKTHYENYIKLYSETLKSQ 146
The following BLAST results are available for this feature:
BLAST of SMED30012619 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
MATR34.463e-632.73matrin 3 [Source:HGNC Symbol;Acc:HGNC:6912][more]
MATR34.646e-632.73matrin 3 [Source:NCBI gene;Acc:9782][more]
MATR34.646e-632.73matrin 3 [Source:NCBI gene;Acc:9782][more]
MATR34.646e-632.73matrin 3 [Source:HGNC Symbol;Acc:HGNC:6912][more]
MATR34.646e-632.73matrin 3 [Source:HGNC Symbol;Acc:HGNC:6912][more]
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BLAST of SMED30012619 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012619 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012619 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
CR352328.15.503e-645.24si:ch211-212k18.4 [Source:NCBI gene;Acc:563749][more]
si:ch211-212k18.45.505e-645.24si:ch211-212k18.4 [Source:ZFIN;Acc:ZDB-GENE-141216... [more]
si:ch211-212k18.45.505e-645.24si:ch211-212k18.4 [Source:ZFIN;Acc:ZDB-GENE-141216... [more]
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BLAST of SMED30012619 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012619 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Matr33.134e-632.73matrin 3 [Source:MGI Symbol;Acc:MGI:1298379][more]
Matr33.134e-632.73matrin 3 [Source:MGI Symbol;Acc:MGI:1298379][more]
Matr33.134e-632.73matrin 3 [Source:MGI Symbol;Acc:MGI:1298379][more]
Matr33.140e-632.73matrin 3 [Source:MGI Symbol;Acc:MGI:1298379][more]
Matr33.234e-632.73matrin 3 [Source:MGI Symbol;Acc:MGI:1298379][more]
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BLAST of SMED30012619 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012619 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012619 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012619 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012619 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012619 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012619 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSORLT00000044190.17.082e-637.50zinc finger protein 638 [Source:NCBI gene;Acc:1011... [more]
ENSORLT00000033229.17.085e-637.50zinc finger protein 638 [Source:NCBI gene;Acc:1011... [more]
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BLAST of SMED30012619 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000006885.12.960e-14088.70SMESG000006885.1[more]
SMESG000078442.19.535e-1145.10SMESG000078442.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012619 ID=SMED30012619|Name=SMED30012619|organism=Schmidtea mediterranea sexual|type=transcript|length=1347bp
TCTGAATATTGATAAATTTTAAGAGTTTAAAACCATTTATTTTTTTAAAG
AATTAATATGAACAGAAATAGATTTGTGCCACCTCCATCGTTTAGTAATG
ACATTTCTTTAAATTCTATGAGCTATGGTGCAAGTATTCCTAGATTTAAC
GTTTGGCAATCCAGTTTAACACCGGTCCAAAATGTACAACAGAGCTATCC
TAATCCACAGGCTAAAAAGAATTATTGCAGTACGTGTGATATGTGGACAT
CAGAACCTTATAATAATCATCAAATATCACCAGCACATCTGGCTAAAAAA
ATTGGTCAACGTGGTAATCAAAATTATCAAACTAGTTATAGCAACACTAA
TTCTAGTTCTTCAATTACATCCAATAATAATCTTTGTAATAATAATAGCA
ATGCAAATCTTAACAAAAGAAAATTTAAGCGTTCTAACAGGCGAGTAAGT
TGTTTAAAATTCAATTCATTTCAGAGAACCGATAATCAGAATCGCAGACA
ATCATCCTCGAATAAATCTATCTCAGAAGAATCGGCTATCAACGATGACC
ATAAAACAGATGCACAAAATTCATCAGATGAGAAAAAAGATGATAAACTG
AAAGAAGACATTAAAATGCTAAATGTTAATAGAAATCGAGGTCAAGAAAT
GTTAATACCCGTTAATGGAGTTTTTTGTGCATTATGTGAAATATTTTTTG
CCAACAAAGATGTAGCTGAAAAGGACCATTGTAGTACACAAGCCCACTGT
GAAGCATACGAGAAACTCCTCAAGCGGGTGCAAGAATCTAAATCCGATAG
GAGGGTGAATGAGAAATCCTCCGATGACAACGGTAATGCTGACAATAGCA
ACGGTGATGGCAATAATAATAAAGATGATGATGATGATGAAAATTGCAAA
CGTTTGAAATTGACGCCCGCCGAGAATCAAGATAAAGATTTCAGAATTCT
CTCATCGATTCCTCACGTGGTGGCAGTGGAGGAGCAGGAGGAGCGGAATT
CCGACCCGATCAACAACGACAGCCAATTATCGGCCGGCAAAGACGGCGAG
GAAATCGATCGGAATTCCACTAATGATTTCAACGCGCCAGATTTCTCGCT
GGATTTCCCCGATGACATGGAGGACTATGTCGATGACATTGTCGATCTGC
CGACCAACTCGAAAACGTCAGCCGAAAAACGCAGCCCTGTTGTCGCTAAT
AGTAACAGTAATAGTAATAATAAATCAAAAATCAATAAATAGTGAGGATG
AGCACGCGCCAATCAATATATATATAAATAATAATCGTAATTATTTCTCT
GTGAATTTTCCATTAATAAAATCAATATTATTATTATTATTATTATC
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protein sequence of SMED30012619-orf-1

>SMED30012619-orf-1 ID=SMED30012619-orf-1|Name=SMED30012619-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=395bp
MNRNRFVPPPSFSNDISLNSMSYGASIPRFNVWQSSLTPVQNVQQSYPNP
QAKKNYCSTCDMWTSEPYNNHQISPAHLAKKIGQRGNQNYQTSYSNTNSS
SSITSNNNLCNNNSNANLNKRKFKRSNRRVSCLKFNSFQRTDNQNRRQSS
SNKSISEESAINDDHKTDAQNSSDEKKDDKLKEDIKMLNVNRNRGQEMLI
PVNGVFCALCEIFFANKDVAEKDHCSTQAHCEAYEKLLKRVQESKSDRRV
NEKSSDDNGNADNSNGDGNNNKDDDDDENCKRLKLTPAENQDKDFRILSS
IPHVVAVEEQEERNSDPINNDSQLSAGKDGEEIDRNSTNDFNAPDFSLDF
PDDMEDYVDDIVDLPTNSKTSAEKRSPVVANSNSNSNNKSKINK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: molecular function
TermDefinition
GO:0000166nucleotide binding
GO:0003674molecular_function
GO:0003676nucleic acid binding
GO:0003723RNA binding
GO:0008270zinc ion binding
GO:0046872metal ion binding
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005737cytoplasm
GO:0016363nuclear matrix
Vocabulary: biological process
TermDefinition
GO:0008150biological_process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 134..180
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 158..180
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 242..256
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 134..157
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 242..277