SMED30012612
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30012612 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Alignments
SMED30012612 aligns in the following genomic locations:
Homology
BLAST of SMED30012612 vs. Planmine SMEST
Match: SMESG000027091.1 (SMESG000027091.1) HSP 1 Score: 516.153 bits (1328), Expect = 0.000e+0 Identity = 321/326 (98.47%), Postives = 322/326 (98.77%), Query Frame = 3 Query: 3 MLLRFSKYFDSISPYRLEIYKDAIISMFLFSISVAIIQNYSIYTLRVTPYTVKEIAYWQSILAITTILFIINVMLAFLNSRINYINRNFFQKGPTENPKIVYLICLPLIVIIXXXXXXXXXXXXXXVDCXXXXXXXXXXXXXXXXXXXXXXXXNILSSSNTGELPLVNIVNAFTLLISLASIWNLTVYNSIYMLSADFKITVSSVFNDSAFKNECRIRNVADNKWAXXXXXXXXXXXXXXXXMLGICLTPYFLKPIQSHFSFINKSILKSISCIEEENPSRINYNRYYSVLILFALCFTFYVGQKLYPSTNFYEIELILRLCILVS 980 MLLRFSKYFDSISPYRLEIYKDAIISMFLFSISVAIIQNYSIYTLRVTPYTVKEIAYWQSILAITTILFIINVMLAFLNSRINYINRNFFQKGPTENPKIVYLICLPLIVIIFSSLSYTTLFLFLYVDCGSVIDIVFSGSIVIFFISMMFVIINILSSSNTGELPLVNIVNAFTLLISLASIWNLTVYNSIYMLSADFK TV VFNDSAFKNECRIRNV DNKWAINLNSILLSSLNISILMLGICLTPYFLKPIQSHFSFINKSI+KSISCIEEENPSRINYNRYYSVLILFALCFTFYVGQKLYPSTNFYEIELILRLCILVS Sbjct: 1 MLLRFSKYFDSISPYRLEIYKDAIISMFLFSISVAIIQNYSIYTLRVTPYTVKEIAYWQSILAITTILFIINVMLAFLNSRINYINRNFFQKGPTENPKIVYLICLPLIVIIFSSLSYTTLFLFLYVDCGSVIDIVFSGSIVIFFISMMFVIINILSSSNTGELPLVNIVNAFTLLISLASIWNLTVYNSIYMLSADFKRTVRIVFNDSAFKNECRIRNVTDNKWAINLNSILLSSLNISILMLGICLTPYFLKPIQSHFSFINKSIIKSISCIEEENPSRINYNRYYSVLILFALCFTFYVGQKLYPSTNFYEIELILRLCILVS 326 HSP 2 Score: 262.692 bits (670), Expect = 0.000e+0 Identity = 173/181 (95.58%), Postives = 175/181 (96.69%), Query Frame = 1 Query: 961 VCAFWCPNLVTIVVMRDLKYVSTVTKKESRETYYIILSIGIIGEFATIIQQLILKINTIFYSTNQSIVTSIXXXXXXXXXXXXXXXQSMLLLVIIKVNSEYETIMTRSNIYILSLILCLYNGFYFIYEISSLNQYYENAIGFNIHLFEVGLLKISIPXXXXXXXXXXXXXXXHFISVLRSK 1503 +C NLVTIVVMRDLKYVSTVTKKESRETYYIILSIGIIGEFATIIQQLILKINTIFYSTNQSIVTSILLFGFFGLSLVNGFLQSMLLLVIIKVNSEYETIM RSNIYILSLILCLYNGFYFIYEISSLNQYYENAIGFNIHLFEVGLLKISIPLKLLYRLFSSMLLLKHFIS+LRSK Sbjct: 321 LCILVSINLVTIVVMRDLKYVSTVTKKESRETYYIILSIGIIGEFATIIQQLILKINTIFYSTNQSIVTSILLFGFFGLSLVNGFLQSMLLLVIIKVNSEYETIMKRSNIYILSLILCLYNGFYFIYEISSLNQYYENAIGFNIHLFEVGLLKISIPLKLLYRLFSSMLLLKHFISILRSK 501
BLAST of SMED30012612 vs. Planmine SMEST
Match: SMESG000027096.1 (SMESG000027096.1) HSP 1 Score: 504.982 bits (1299), Expect = 0.000e+0 Identity = 325/326 (99.69%), Postives = 326/326 (100.00%), Query Frame = 3 Query: 3 MLLRFSKYFDSISPYRLEIYKDAIISMFLFSISVAIIQNYSIYTLRVTPYTVKEIAYWQSILAITTILFIINVMLAFLNSRINYINRNFFQKGPTENPKIVYLICLPLIVIIXXXXXXXXXXXXXXVDCXXXXXXXXXXXXXXXXXXXXXXXXNILSSSNTGELPLVNIVNAFTLLISLASIWNLTVYNSIYMLSADFKITVSSVFNDSAFKNECRIRNVADNKWAXXXXXXXXXXXXXXXXMLGICLTPYFLKPIQSHFSFINKSILKSISCIEEENPSRINYNRYYSVLILFALCFTFYVGQKLYPSTNFYEIELILRLCILVS 980 MLLRFSKYFDSISPYRLEIYKDAIISMFLFSISVAIIQNYSIYTLRVTPYTVKEIAYWQSILAITTILFIINVMLAFLNSRINYINRNFFQKGPTENPKIVYLICLPLI+IIFSSLSYTTLFLFLYVDCGSVIDIVFSGSIVIFFISMMFVIINILSSSNTGELPLVNIVNAFTLLISLASIWNLTVYNSIYMLSADFKITVSSVFNDSAFKNECRIRNVADNKWAINLNSILLSSLNISILMLGICLTPYFLKPIQSHFSFINKSILKSISCIEEENPSRINYNRYYSVLILFALCFTFYVGQKLYPSTNFYEIELILRLCILVS Sbjct: 1 MLLRFSKYFDSISPYRLEIYKDAIISMFLFSISVAIIQNYSIYTLRVTPYTVKEIAYWQSILAITTILFIINVMLAFLNSRINYINRNFFQKGPTENPKIVYLICLPLIIIIFSSLSYTTLFLFLYVDCGSVIDIVFSGSIVIFFISMMFVIINILSSSNTGELPLVNIVNAFTLLISLASIWNLTVYNSIYMLSADFKITVSSVFNDSAFKNECRIRNVADNKWAINLNSILLSSLNISILMLGICLTPYFLKPIQSHFSFINKSILKSISCIEEENPSRINYNRYYSVLILFALCFTFYVGQKLYPSTNFYEIELILRLCILVS 326 HSP 2 Score: 265.388 bits (677), Expect = 0.000e+0 Identity = 175/181 (96.69%), Postives = 176/181 (97.24%), Query Frame = 1 Query: 961 VCAFWCPNLVTIVVMRDLKYVSTVTKKESRETYYIILSIGIIGEFATIIQQLILKINTIFYSTNQSIVTSIXXXXXXXXXXXXXXXQSMLLLVIIKVNSEYETIMTRSNIYILSLILCLYNGFYFIYEISSLNQYYENAIGFNIHLFEVGLLKISIPXXXXXXXXXXXXXXXHFISVLRSK 1503 +C NLVTIVVMRDLKYVSTVTKKESRETYYIILSIGIIGEFATIIQQLILKINTIFYSTNQSIVTSILLFGFFGLSLVNGFLQSMLLLVIIKVNSEYETIMTRSNIYILSLILCLYNGFYFIYEISSLNQYYENAIGFNIHLFEVGLLKISIPLKLLYRLFSSMLLLKHFISVLRSK Sbjct: 321 LCILVSINLVTIVVMRDLKYVSTVTKKESRETYYIILSIGIIGEFATIIQQLILKINTIFYSTNQSIVTSILLFGFFGLSLVNGFLQSMLLLVIIKVNSEYETIMTRSNIYILSLILCLYNGFYFIYEISSLNQYYENAIGFNIHLFEVGLLKISIPLKLLYRLFSSMLLLKHFISVLRSK 501
BLAST of SMED30012612 vs. Planmine SMEST
Match: SMESG000027093.1 (SMESG000027093.1) HSP 1 Score: 251.521 bits (641), Expect = 2.372e-90 Identity = 168/319 (52.66%), Postives = 231/319 (72.41%), Query Frame = 3 Query: 18 SKYFDSISPYRLEIYKDAIISMFLFSISVAIIQNYSIYTLRVTPYTVKEIAYWQSILAITTILFIINVMLAFLNSRINYINRNFFQKGPTENPKIVYLICLPLIVIIXXXXXXXXXXXXXXVDCXXXXXXXXXXXXXXXXXXXXXXXXNILSSSNTGELPLVNIVNAFTLLISLASIWNLTVYNSIYMLSADFKITVSSVFNDSAFKNECRIRNVADNKWAXXXXXXXXXXXXXXXXMLGICLTPYFLKPIQSHFSFINKSILKSISCIEEENPSRINYNRYYSVLILFALCFTFYVGQKLYPSTNFYEIELILRLCIL 974 K DS S ++ I K +I++ F+F++ V I+ NY+IYTL +TPYT+ I YW++IL I+TILF++NV L N R+NY+NRNFF KGPTENPKIVYLIC PLI++I SSLSYT FLF + DCGS IDI+F+GSI++FF SM+F+IIN+LSSS+TGELPLVNI+NAFTL++SLAS+WNLT+YN +L+ FK TV VFN++ F N+C +N+ ++ W ++L+ +L L IS ++LGICL PY +P++SH SFINKSI + + E SR NY YY +++ L F ++ +K PS I L L C++ Sbjct: 6 GKSSDSYSFHKYFIIKYSIVTAFMFTVGVGIVWNYTIYTLSLTPYTIIRIEYWKTILFISTILFLLNVGLILTNYRVNYMNRNFFLKGPTENPKIVYLICFPLIILILSSLSYTAAFLFHFNDCGSFIDIIFAGSIILFFTSMIFLIINVLSSSDTGELPLVNIINAFTLMVSLASVWNLTLYNIELVLTNSFKTTVKRVFNEATFFNQCSNKNIPNSPWPVDLDVNMLPLLRISSIILGICLMPYLFRPLKSHISFINKSIPNASHYFDVEFVSRKNYKNYYLSIVMIVLNFAIFIAKKFSPSNIIDTIALFLFFCVM 324 HSP 2 Score: 101.293 bits (251), Expect = 2.372e-90 Identity = 61/141 (43.26%), Postives = 91/141 (64.54%), Query Frame = 1 Query: 1009 DLKYVSTVTKKESRETYYIILSIGIIGEFATIIQQLILKINTIFYSTNQSIVTSIXXXXXXXXXXXXXXXQSMLLLVIIKVNSEYETIMTRSNIYILSLILCLYNGFYFIYEISSLNQYYENAIGFNIHLFEVGLLKISIP 1431 D++Y+ T + T+YI L IG+IGEF ++I++LIL I ++Y N S++ S++ GL L+NGF+QS+LL ++IK SEYE + +NIYIL L+LC YNG YFIY + ++ Y + FNI GL +I +P Sbjct: 337 DMQYIPTTGNGSASVTHYIFLWIGVIGEFVSVIERLILCIYNLYYGPNNSLILSVINLCNVGLLLINGFVQSVLLSIVIKDKSEYEVNKSGTNIYILPLVLCFYNGLYFIYNLFLVDPCYYHCNVFNIAFLTSGLYQICLP 477
BLAST of SMED30012612 vs. Planmine SMEST
Match: SMESG000025369.1 (SMESG000025369.1) HSP 1 Score: 56.6102 bits (135), Expect = 2.824e-8 Identity = 32/84 (38.10%), Postives = 48/84 (57.14%), Query Frame = 3 Query: 72 IISMFLFSISVAIIQNYSIYTLRVTPYTVKEIAYWQSILAITTILFIINVMLAFLNSRINYINRNFFQKGPTENPKIVYLICLP 323 +IS+ L S+ + NYS++ +R TP + I + I I+ + N++L N+RINY RN + P E P I+YLICLP Sbjct: 51 MISIKLLIFSIVYLTNYSLFIVRFTPLKDRCIGIPKIITCAMVIVKLTNILLIIYNARINYRFRNLRIRNPVERPMILYLICLP 134
BLAST of SMED30012612 vs. Planmine SMEST
Match: SMESG000027122.1 (SMESG000027122.1) HSP 1 Score: 56.225 bits (134), Expect = 3.213e-8 Identity = 52/192 (27.08%), Postives = 101/192 (52.60%), Query Frame = 3 Query: 54 EIYKDAIISMFLFSISVAIIQNYSIYTLRVTPYTVKEIAYWQSILAITTILFIINVMLAFLNSRINYINRNFFQKGPTENPKIVYLICLPLIVIIXXXXXXXXXXXXXXVDCXXXXXXXXXXXXXXXXXXXXXXXXNILSSSNTGELPLVNIVNAFTLLISLASIWNLTVYNSIYMLSADFKITVSSVFNDS 629 EI + I +LF++S++I+ YS + + T + ++ I IL ++NV+L N+++NY RN E P + YLICLPL+++I +L+ T+ + ++ G F +++ +I++I S+TGE P +N VN+ T++++L S+ ++ S +F + ++F +S Sbjct: 3 EIVEKLIACAYLFTVSLSILLTYSTFIILNTQVQERCSYCFEFIQWPVNILMMVNVLLIVYNTKMNYKYRNLRFSLQIEQPLVFYLICLPLLLVILVALTLLTINSISRWFATRIGFVIEIGLAATEFFAVIILILDISGFSDTGEEPFINSVNSVTIIVTLISMG--------FLFSKNFILKSRNIFKNS 186 The following BLAST results are available for this feature:
BLAST of SMED30012612 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30012612 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30012612 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30012612 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30012612 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30012612 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30012612 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30012612 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30012612 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30012612 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30012612 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30012612 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30012612 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30012612 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30012612 ID=SMED30012612|Name=SMED30012612|organism=Schmidtea mediterranea sexual|type=transcript|length=1517bpback to top protein sequence of SMED30012612-orf-1 >SMED30012612-orf-1 ID=SMED30012612-orf-1|Name=SMED30012612-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=168bp MRDLKYVSTVTKKESRETYYIILSIGIIGEFATIIQQLILKINTIFYSTNback to top protein sequence of SMED30012612-orf-2 >SMED30012612-orf-2 ID=SMED30012612-orf-2|Name=SMED30012612-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=345bp MLLRFSKYFDSISPYRLEIYKDAIISMFLFSISVAIIQNYSIYTLRVTPYback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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