SMED30012606

Overview
NameSMED30012606
Smed IDSMED30012606
Length (bp)2709
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30012606 (SMED30012606) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30012606 (SMED30012606) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30012606 (SMED30012606) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30012606 (SMED30012606) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30012606

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30012606SMESG000054635.1 SMESG000054602.1 SMESG000052914.1 SmedASXL_017615SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30012606SMESG000054635.1 SMESG000054602.1 SMESG000052914.1 SmedASXL_017615SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X1 cellSMED30012606SMESG000054602.1 SmedASXL_076832SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30012606SMESG000054602.1 SmedASXL_076832SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
pharynxSMED30012606SMESG000054635.1 SMESG000054602.1 SMESG000052914.1 dd_Smed_v4_2185_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30012606 vs. Planmine SMEST
Match: SMESG000052914.1 (SMESG000052914.1)

HSP 1 Score: 1107.43 bits (2863), Expect = 0.000e+0
Identity = 559/640 (87.34%), Postives = 583/640 (91.09%), Query Frame = 1 
Query:   28 MNTNLILVTNIPKHIKLFDLIKELENKWARLIFIDNPYACLYDAFIYNPTSLLTHFEMGVSRLRNKVINGAKPLKVYHLWFPPPNVYLTSLVIIFLIMKVTRKSFASYLTC*NIFL*KLNVXXXXXXXXXXXPENATMLPVIFVDNLPKECDSYEIIGDLPFQTNEIKHWDIPQKEDKWMILRLPVNQDEAIRDYVLKYIRTRRLSNGLFPRVLEVFVSEDIRLSKNMVPKTLQILPITTSFKSNLFFLYDLPTKLTVKAISDAAKAIGVKGSKAFFVDVVDSLRVGFLFVPKSFPDSKFDSFQEALKEKEIVVDSNQTVMLNLNEVYLQPKGALPPKVHRKRDDERTIEYVLTNYVYVHNVAACIDHNQLTKGSPINFKVYLFQINENTTQVGFVCLRGLSDVGISQLIDHLRSLVFEGFQVMVDNIYLKCYDMPDNNSERRAVHVPREPLDNVDNITSHVDNTFVDITEINTXXXXXXXXXXXXXXXXXXLAEFSVEISSDPPPEYNLMKSDFIFVNYLPNTVEYSSFSLLPLECDSLTIMSCDEHKIAFLYYPDTTTDKWMGALNKLVTTKIKETNVEVSEVYLQSDENEEMLDKGLDMISPSNYVFVYNIGDCSDTRKLEGNTNIVRFSTMTQESG 1947
            MNTNLILVTNIPKHI+ FDLIKELENKWARLIFIDNPYA L +AFIYNPTSLLTHFEMGVSRLR+KVING +PLKVYHLWFPPPNVYLT+L+                                        PENATMLPV+FVDNLPKECDSYE+IGDLPFQ+N++KHWDIPQKEDKWM+LRLPVNQDEAIRDYVLKYIRTRRLSNGLFPRVLEVFVSEDIRLSKNMVPKTLQILPIT SFKSNL FLYDLPTKLTVK ISDAAKA+GVKGSKAFF+DVVDSLRVGFLFVPKSFPDSKF+ FQEALKEKEIVVDSNQTVMLNLNEVYLQPKGALPPKVH+KRDD+RTIEYVLTNYVYVHNVAA IDHNQL KGS INFK+YLFQI ENTTQVGFVCLRGLSDV ISQLIDHLRSLVFEGFQVMVDNIYLKCYDMPDNNSERR VHVPREPLDNVDNITSHVDNTFVDITE+N EESEVEVID+D+E+D+E+L E SVEISSDPPPEYNLMKSDFIFVNYLPNTVEYSSFSLLPLECDSLTIMSCDEHKIAFLYYPDTTTDKWMGALNKLVTTKIKETNVEVSEVYLQSDENEEMLDKGLDMISPSNYVFVYNIGDCSDTRKLEGNTNIVRFSTMTQESG
Sbjct:    1 MNTNLILVTNIPKHIRHFDLIKELENKWARLIFIDNPYADLQEAFIYNPTSLLTHFEMGVSRLRSKVINGIRPLKVYHLWFPPPNVYLTNLI----------------------------------------PENATMLPVLFVDNLPKECDSYEVIGDLPFQSNDVKHWDIPQKEDKWMVLRLPVNQDEAIRDYVLKYIRTRRLSNGLFPRVLEVFVSEDIRLSKNMVPKTLQILPITASFKSNLLFLYDLPTKLTVKGISDAAKALGVKGSKAFFIDVVDSLRVGFLFVPKSFPDSKFEFFQEALKEKEIVVDSNQTVMLNLNEVYLQPKGALPPKVHKKRDDDRTIEYVLTNYVYVHNVAAGIDHNQLAKGSTINFKIYLFQIFENTTQVGFVCLRGLSDVAISQLIDHLRSLVFEGFQVMVDNIYLKCYDMPDNNSERRTVHVPREPLDNVDNITSHVDNTFVDITEVNLEESEVEVIDTDEENDIEMLKECSVEISSDPPPEYNLMKSDFIFVNYLPNTVEYSSFSLLPLECDSLTIMSCDEHKIAFLYYPDTTTDKWMGALNKLVTTKIKETNVEVSEVYLQSDENEEMLDKGLDMISPSNYVFVYNIGDCSDTRKLEGNTNIVRFSTMTQESG 600
[Close]
BLAST of SMED30012606 vs. Planmine SMEST
Match: SMESG000052914.1 (SMESG000052914.1)

HSP 1 Score: 1100.5 bits (2845), Expect = 0.000e+0
Identity = 559/649 (86.13%), Postives = 583/649 (89.83%), Query Frame = 1 
Query:   28 MNTNLILVTNIPKHIKLFDLIKELENKWARLIFIDNPYACLYDAFIYNPTSLLTHFEMGVSRLRNKVINGAKPLKVYHLWFPPPNVYLTSLVIIFLIMKVTRKSFASYLTC*NIFL*KLNVXXXXXXXXXXXPENATMLPVIFVDNLPKECDSYEIIGDLPFQTNEIKHWDIPQKEDK---------WMILRLPVNQDEAIRDYVLKYIRTRRLSNGLFPRVLEVFVSEDIRLSKNMVPKTLQILPITTSFKSNLFFLYDLPTKLTVKAISDAAKAIGVKGSKAFFVDVVDSLRVGFLFVPKSFPDSKFDSFQEALKEKEIVVDSNQTVMLNLNEVYLQPKGALPPKVHRKRDDERTIEYVLTNYVYVHNVAACIDHNQLTKGSPINFKVYLFQINENTTQVGFVCLRGLSDVGISQLIDHLRSLVFEGFQVMVDNIYLKCYDMPDNNSERRAVHVPREPLDNVDNITSHVDNTFVDITEINTXXXXXXXXXXXXXXXXXXLAEFSVEISSDPPPEYNLMKSDFIFVNYLPNTVEYSSFSLLPLECDSLTIMSCDEHKIAFLYYPDTTTDKWMGALNKLVTTKIKETNVEVSEVYLQSDENEEMLDKGLDMISPSNYVFVYNIGDCSDTRKLEGNTNIVRFSTMTQESG 1947
            MNTNLILVTNIPKHI+ FDLIKELENKWARLIFIDNPYA L +AFIYNPTSLLTHFEMGVSRLR+KVING +PLKVYHLWFPPPNVYLT+L+                                        PENATMLPV+FVDNLPKECDSYE+IGDLPFQ+N++KHWDIPQKEDK         WM+LRLPVNQDEAIRDYVLKYIRTRRLSNGLFPRVLEVFVSEDIRLSKNMVPKTLQILPIT SFKSNL FLYDLPTKLTVK ISDAAKA+GVKGSKAFF+DVVDSLRVGFLFVPKSFPDSKF+ FQEALKEKEIVVDSNQTVMLNLNEVYLQPKGALPPKVH+KRDD+RTIEYVLTNYVYVHNVAA IDHNQL KGS INFK+YLFQI ENTTQVGFVCLRGLSDV ISQLIDHLRSLVFEGFQVMVDNIYLKCYDMPDNNSERR VHVPREPLDNVDNITSHVDNTFVDITE+N EESEVEVID+D+E+D+E+L E SVEISSDPPPEYNLMKSDFIFVNYLPNTVEYSSFSLLPLECDSLTIMSCDEHKIAFLYYPDTTTDKWMGALNKLVTTKIKETNVEVSEVYLQSDENEEMLDKGLDMISPSNYVFVYNIGDCSDTRKLEGNTNIVRFSTMTQESG
Sbjct:    1 MNTNLILVTNIPKHIRHFDLIKELENKWARLIFIDNPYADLQEAFIYNPTSLLTHFEMGVSRLRSKVINGIRPLKVYHLWFPPPNVYLTNLI----------------------------------------PENATMLPVLFVDNLPKECDSYEVIGDLPFQSNDVKHWDIPQKEDKYAIFNMLVKWMVLRLPVNQDEAIRDYVLKYIRTRRLSNGLFPRVLEVFVSEDIRLSKNMVPKTLQILPITASFKSNLLFLYDLPTKLTVKGISDAAKALGVKGSKAFFIDVVDSLRVGFLFVPKSFPDSKFEFFQEALKEKEIVVDSNQTVMLNLNEVYLQPKGALPPKVHKKRDDDRTIEYVLTNYVYVHNVAAGIDHNQLAKGSTINFKIYLFQIFENTTQVGFVCLRGLSDVAISQLIDHLRSLVFEGFQVMVDNIYLKCYDMPDNNSERRTVHVPREPLDNVDNITSHVDNTFVDITEVNLEESEVEVIDTDEENDIEMLKECSVEISSDPPPEYNLMKSDFIFVNYLPNTVEYSSFSLLPLECDSLTIMSCDEHKIAFLYYPDTTTDKWMGALNKLVTTKIKETNVEVSEVYLQSDENEEMLDKGLDMISPSNYVFVYNIGDCSDTRKLEGNTNIVRFSTMTQESG 609
[Close]
BLAST of SMED30012606 vs. Planmine SMEST
Match: SMESG000054602.1 (SMESG000054602.1)

HSP 1 Score: 1051.97 bits (2719), Expect = 0.000e+0
Identity = 540/583 (92.62%), Postives = 542/583 (92.97%), Query Frame = 1 
Query:  199 MGVSRLRNKVINGAKPLKVYHLWFPPPNVYLTSLVIIFLIMKVTRKSFASYLTC*NIFL*KLNVXXXXXXXXXXXPENATMLPVIFVDNLPKECDSYEIIGDLPFQTNEIKHWDIPQKEDKWMILRLPVNQDEAIRDYVLKYIRTRRLSNGLFPRVLEVFVSEDIRLSKNMVPKTLQILPITTSFKSNLFFLYDLPTKLTVKAISDAAKAIGVKGSKAFFVDVVDSLRVGFLFVPKSFPDSKFDSFQEALKEKEIVVDSNQTVMLNLNEVYLQPKGALPPKVHRKRDDERTIEYVLTNYVYVHNVAACIDHNQLTKGSPINFKVYLFQINENTTQVGFVCLRGLSDVGISQLIDHLRSLVFEGFQVMVDNIYLKCYDMPDNNSERRAVHVPREPLDNVDNITSHVDNTFVDITEINTXXXXXXXXXXXXXXXXXXLAEFSVEISSDPPPEYNLMKSDFIFVNYLPNTVEYSSFSLLPLECDSLTIMSCDEHKIAFLYYPDTTTDKWMGALNKLVTTKIKETNVEVSEVYLQSDENEEMLDKGLDMISPSNYVFVYNIGDCSDTRKLEGNTNIVRFSTMTQESG 1947
            MGVSRLRNKVINGAKPLKVYHLWFPPPNVYLTSL+                                        PENATMLPVIFVDNLPKECDSYEIIGDLPFQTNEIKHWDIPQKEDKWMILRLPVNQDEAIRDYVLKYIRTRRLSNGLFPRVLEVFVSEDIRLSKNMVPKTLQILPITTSFKSNLFFLYDLPTKLTVKAISDAAKA+GVKGSKAFFVDVVDSLRVGFLFVPKSFPDSKFDSFQEALKEKEIVVDSNQTVMLNLNEVYLQPKGALPPKVHRKRDDERTIEY LTNYVYVHNVAACIDHNQLTKGSPINFKVYLFQINENTTQVGFVCLRGLSDVGISQLIDHLRSLVFEGFQVMVDNIYLKCYDMPDNNSERRAVHVPREPLDNVDNITSHVDNTFVDITEINTEESEVEVIDSDDEDDVEILAEFSVEISSDPPPEYNLMKSDFIFVNYLPNTVEYSSFSLLPLECDSLTIMSCDEHKIAFLYYPDTTTDKWMGALNKLVTTKIKETNVEVSEVYLQSDENEEMLDKGLDMISPSNYVFVYNIGDCSDTRKLEGNTNIVRFSTMTQESG
Sbjct:    1 MGVSRLRNKVINGAKPLKVYHLWFPPPNVYLTSLI----------------------------------------PENATMLPVIFVDNLPKECDSYEIIGDLPFQTNEIKHWDIPQKEDKWMILRLPVNQDEAIRDYVLKYIRTRRLSNGLFPRVLEVFVSEDIRLSKNMVPKTLQILPITTSFKSNLFFLYDLPTKLTVKAISDAAKALGVKGSKAFFVDVVDSLRVGFLFVPKSFPDSKFDSFQEALKEKEIVVDSNQTVMLNLNEVYLQPKGALPPKVHRKRDDERTIEYALTNYVYVHNVAACIDHNQLTKGSPINFKVYLFQINENTTQVGFVCLRGLSDVGISQLIDHLRSLVFEGFQVMVDNIYLKCYDMPDNNSERRAVHVPREPLDNVDNITSHVDNTFVDITEINTEESEVEVIDSDDEDDVEILAEFSVEISSDPPPEYNLMKSDFIFVNYLPNTVEYSSFSLLPLECDSLTIMSCDEHKIAFLYYPDTTTDKWMGALNKLVTTKIKETNVEVSEVYLQSDENEEMLDKGLDMISPSNYVFVYNIGDCSDTRKLEGNTNIVRFSTMTQESG 543
[Close]
BLAST of SMED30012606 vs. Planmine SMEST
Match: SMESG000054635.1 (SMESG000054635.1)

HSP 1 Score: 1013.45 bits (2619), Expect = 0.000e+0
Identity = 523/640 (81.72%), Postives = 546/640 (85.31%), Query Frame = 1 
Query:   28 MNTNLILVTNIPKHIKLFDLIKELENKWARLIFIDNPYACLYDAFIYNPTSLLTHFEMGVSRLRNKVINGAKPLKVYHLWFPPPNVYLTSLVIIFLIMKVTRKSFASYLTC*NIFL*KLNVXXXXXXXXXXXPENATMLPVIFVDNLPKECDSYEIIGDLPFQTNEIKHWDIPQKEDKWMILRLPVNQDEAIRDYVLKYIRTRRLSNGLFPRVLEVFVSEDIRLSKNMVPKTLQILPITTSFKSNLFFLYDLPTKLTVKAISDAAKAIGVKGSKAFFVDVVDSLRVGFLFVPKSFPDSKFDSFQEALKEKEIVVDSNQTVMLNLNEVYLQPKGALPPKVHRKRDDERTIEYVLTNYVYVHNVAACIDHNQLTKGSPINFKVYLFQINENTTQVGFVCLRGLSDVGISQLIDHLRSLVFEGFQVMVDNIYLKCYDMPDNNSERRAVHVPREPLDNVDNITSHVDNTFVDITEINTXXXXXXXXXXXXXXXXXXLAEFSVEISSDPPPEYNLMKSDFIFVNYLPNTVEYSSFSLLPLECDSLTIMSCDEHKIAFLYYPDTTTDKWMGALNKLVTTKIKETNVEVSEVYLQSDENEEMLDKGLDMISPSNYVFVYNIGDCSDTRKLEGNTNIVRFSTMTQESG 1947
            MNTNLILVTNIPKHI+ FDLIKELENKWARLIFIDNPYA L +AFIYNPTSLLTHFEMGVSRLR+KVING +PLKVYHLWFPPPNVYLT+L+                                        PENATMLPV+FVDNLPKECDSYE+IGDLPFQ+N++KHWDIPQKEDKWM+LRLPVNQDEAIRDYVLKYIRTRRLSNGLFPRVLEVFVSEDIRLSKNMVPKTLQILPIT SFKSNL FLYDLPTKLTVK ISDAAKA+GVKGSKAFF+DVVDSLRVGFLFVPKSFPDSKF+ FQEALKEKEIVVDSNQTVMLNLNEVYLQPKGALPPKVH+KRDDERTIEYVLTNYVYVHNVAA IDH+QL KGS INFK+YLFQI ENTTQVGFVCLRGLSD                                      RR VHVPREPLDNVDNITSHVDNTFVDITE+N EESEVEVIDSD+E+D+E+L E SVEISSDPPPEYNLMKSDFIFVNYLPNTVEYSSFSLLPLECDSLTIMSCDEHKIAFLYYPDTTTDKWMGALNKLVTTKIKETNVEVSEVYLQSDENEEMLDKGLDMISPSNYVFVYNIGDCSDTRKLEGNTNIVRFSTMTQESG
Sbjct:    1 MNTNLILVTNIPKHIRHFDLIKELENKWARLIFIDNPYADLQEAFIYNPTSLLTHFEMGVSRLRSKVINGIRPLKVYHLWFPPPNVYLTNLI----------------------------------------PENATMLPVLFVDNLPKECDSYEVIGDLPFQSNDVKHWDIPQKEDKWMVLRLPVNQDEAIRDYVLKYIRTRRLSNGLFPRVLEVFVSEDIRLSKNMVPKTLQILPITASFKSNLLFLYDLPTKLTVKGISDAAKALGVKGSKAFFIDVVDSLRVGFLFVPKSFPDSKFEFFQEALKEKEIVVDSNQTVMLNLNEVYLQPKGALPPKVHKKRDDERTIEYVLTNYVYVHNVAAGIDHSQLAKGSTINFKIYLFQIFENTTQVGFVCLRGLSD------------------------------------DSRRTVHVPREPLDNVDNITSHVDNTFVDITEVNLEESEVEVIDSDEENDIEMLKECSVEISSDPPPEYNLMKSDFIFVNYLPNTVEYSSFSLLPLECDSLTIMSCDEHKIAFLYYPDTTTDKWMGALNKLVTTKIKETNVEVSEVYLQSDENEEMLDKGLDMISPSNYVFVYNIGDCSDTRKLEGNTNIVRFSTMTQESG 564
[Close]
BLAST of SMED30012606 vs. Planmine SMEST
Match: SMESG000054635.1 (SMESG000054635.1)

HSP 1 Score: 1006.13 bits (2600), Expect = 0.000e+0
Identity = 525/649 (80.89%), Postives = 549/649 (84.59%), Query Frame = 1 
Query:   28 MNTNLILVTNIPKHIKLFDLIKELENKWARLIFIDNPYACLYDAFIYNPTSLLTHFEMGVSRLRNKVINGAKPLKVYHLWFPPPNVYLTSLVIIFLIMKVTRKSFASYLTC*NIFL*KLNVXXXXXXXXXXXPENATMLPVIFVDNLPKECDSYEIIGDLPFQTNEIKHWDIPQKEDK---------WMILRLPVNQDEAIRDYVLKYIRTRRLSNGLFPRVLEVFVSEDIRLSKNMVPKTLQILPITTSFKSNLFFLYDLPTKLTVKAISDAAKAIGVKGSKAFFVDVVDSLRVGFLFVPKSFPDSKFDSFQEALKEKEIVVDSNQTVMLNLNEVYLQPKGALPPKVHRKRDDERTIEYVLTNYVYVHNVAACIDHNQLTKGSPINFKVYLFQINENTTQVGFVCLRGLSDVGISQLIDHLRSLVFEGFQVMVDNIYLKCYDMPDNNSERRAVHVPREPLDNVDNITSHVDNTFVDITEINTXXXXXXXXXXXXXXXXXXLAEFSVEISSDPPPEYNLMKSDFIFVNYLPNTVEYSSFSLLPLECDSLTIMSCDEHKIAFLYYPDTTTDKWMGALNKLVTTKIKETNVEVSEVYLQSDENEEMLDKGLDMISPSNYVFVYNIGDCSDTRKLEGNTNIVRFSTMTQESG 1947
            MNTNLILVTNIPKHI+ FDLIKELENKWARLIFIDNPYA L +AFIYNPTSLLTHFEMGVSRLR+KVING +PLKVYHLWFPPPNVYLT+L+                                        PENATMLPV+FVDNLPKECDSYE+IGDLPFQ+N++KHWDIPQKEDK         WM+LRLPVNQDEAIRDYVLKYIRTRRLSNGLFPRVLEVFVSEDIRLSKNMVPKTLQILPIT SFKSNL FLYDLPTKLTVK ISDAAKA+GVKGSKAFF+DVVDSLRVGFLFVPKSFPDSKF+ FQEALKEKEIVVDSNQTVMLNLNEVYLQPKGALPPKVH+KRDDERTIEYVLTNYVYVHNVAA IDH+QL KGS INFK+YLFQI ENTTQVGFVCLRGLSD                         +LK          RR VHVPREPLDNVDNITSHVDNTFVDITE+N EESEVEVIDSD+E+D+E+L E SVEISSDPPPEYNLMKSDFIFVNYLPNTVEYSSFSLLPLECDSLTIMSCDEHKIAFLYYPDTTTDKWMGALNKLVTTKIKETNVEVSEVYLQSDENEEMLDKGLDMISPSNYVFVYNIGDCSDTRKLEGNTNIVRFSTMTQESG
Sbjct:    1 MNTNLILVTNIPKHIRHFDLIKELENKWARLIFIDNPYADLQEAFIYNPTSLLTHFEMGVSRLRSKVINGIRPLKVYHLWFPPPNVYLTNLI----------------------------------------PENATMLPVLFVDNLPKECDSYEVIGDLPFQSNDVKHWDIPQKEDKYAIFNMLVKWMVLRLPVNQDEAIRDYVLKYIRTRRLSNGLFPRVLEVFVSEDIRLSKNMVPKTLQILPITASFKSNLLFLYDLPTKLTVKGISDAAKALGVKGSKAFFIDVVDSLRVGFLFVPKSFPDSKFEFFQEALKEKEIVVDSNQTVMLNLNEVYLQPKGALPPKVHKKRDDERTIEYVLTNYVYVHNVAAGIDHSQLAKGSTINFKIYLFQIFENTTQVGFVCLRGLSD-------------------------FLK--------DSRRTVHVPREPLDNVDNITSHVDNTFVDITEVNLEESEVEVIDSDEENDIEMLKECSVEISSDPPPEYNLMKSDFIFVNYLPNTVEYSSFSLLPLECDSLTIMSCDEHKIAFLYYPDTTTDKWMGALNKLVTTKIKETNVEVSEVYLQSDENEEMLDKGLDMISPSNYVFVYNIGDCSDTRKLEGNTNIVRFSTMTQESG 576
The following BLAST results are available for this feature:
BLAST of SMED30012606 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30012606 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30012606 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30012606 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30012606 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30012606 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30012606 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30012606 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30012606 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30012606 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30012606 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30012606 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30012606 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30012606 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000052914.10.000e+087.34SMESG000052914.1[more]
SMESG000052914.10.000e+086.13SMESG000052914.1[more]
SMESG000054602.10.000e+092.62SMESG000054602.1[more]
SMESG000054635.10.000e+081.72SMESG000054635.1[more]
SMESG000054635.10.000e+080.89SMESG000054635.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012606 ID=SMED30012606|Name=SMED30012606|organism=Schmidtea mediterranea sexual|type=transcript|length=2709bp
ATAATACTAGTATACATTTAATTGAATATGAATACAAATCTCATTCTTGT
AACAAATATTCCAAAACATATTAAACTTTTTGATTTAATCAAGGAACTTG
AAAATAAATGGGCTCGTTTAATTTTTATTGATAATCCTTATGCATGCTTG
TACGACGCTTTTATTTATAATCCTACTTCTCTTCTAACTCATTTTGAGAT
GGGTGTGTCGAGGTTGAGAAATAAAGTAATAAATGGTGCAAAACCATTAA
AAGTTTATCATTTATGGTTTCCACCACCAAATGTTTATCTAACTAGCCTG
GTAATTATTTTTTTGATAATGAAAGTAACACGAAAAAGTTTTGCAAGTTA
TTTGACTTGTTAAAATATTTTTCTTTAAAAATTAAATGTGATCTAAATCC
GATCAATATTAATAAAATAGATACCTGAAAATGCTACAATGCTACCAGTT
ATATTTGTGGACAATTTGCCAAAAGAATGTGATTCTTATGAAATAATTGG
CGATTTACCCTTTCAAACAAATGAAATAAAGCATTGGGATATACCTCAAA
AAGAAGATAAATGGATGATTTTACGATTACCTGTAAATCAAGATGAAGCT
ATTAGAGATTATGTGCTGAAATATATTCGAACGAGGCGTTTATCTAATGG
CCTTTTTCCTCGTGTTTTGGAAGTTTTCGTGTCTGAAGACATTCGATTAT
CAAAAAATATGGTTCCTAAAACCTTACAAATTTTACCGATTACTACTTCG
TTTAAATCGAACTTATTTTTTCTTTATGATTTGCCAACAAAGTTGACAGT
TAAAGCAATATCAGATGCTGCTAAAGCTATTGGCGTTAAAGGATCAAAAG
CATTTTTTGTTGACGTCGTTGATTCTTTGAGAGTTGGATTTTTATTCGTT
CCAAAGTCATTTCCTGATTCTAAATTCGATTCCTTCCAAGAAGCTTTGAA
AGAAAAAGAGATTGTCGTAGATAGTAATCAAACAGTAATGCTCAACTTAA
ACGAGGTCTATTTGCAGCCTAAAGGTGCTCTGCCGCCTAAGGTTCACAGG
AAAAGGGACGATGAACGCACAATTGAATATGTGCTTACGAATTATGTATA
TGTTCACAATGTTGCTGCGTGTATAGATCATAATCAATTAACTAAGGGAT
CTCCGATTAATTTCAAAGTTTACTTATTTCAAATTAACGAAAATACGACT
CAAGTTGGATTTGTTTGTTTGAGAGGCCTGTCCGATGTTGGAATTTCTCA
ACTGATCGATCATTTAAGGTCTTTAGTTTTTGAAGGATTCCAAGTAATGG
TGGACAACATCTATTTGAAATGTTATGACATGCCTGATAATAATTCTGAA
AGGCGAGCAGTTCACGTTCCTCGTGAACCCCTGGATAATGTAGATAACAT
AACATCACACGTTGATAACACTTTTGTCGATATCACTGAAATCAATACCG
AGGAAAGTGAAGTAGAAGTTATTGATAGCGATGACGAAGACGATGTTGAA
ATTTTAGCGGAGTTTTCTGTCGAAATATCAAGCGATCCACCTCCGGAGTA
TAACCTAATGAAGTCAGATTTTATTTTCGTTAACTATTTACCGAACACAG
TTGAATATTCGTCATTTTCCCTGTTGCCCCTTGAGTGCGATTCATTAACT
ATAATGTCTTGCGATGAACACAAGATTGCGTTTTTGTATTATCCAGATAC
AACAACGGATAAATGGATGGGAGCACTCAATAAATTAGTTACAACTAAAA
TCAAAGAAACAAATGTAGAAGTTTCTGAGGTATATTTGCAATCGGATGAA
AATGAAGAGATGCTAGATAAAGGATTGGATATGATTTCTCCTAGCAATTA
CGTTTTTGTGTACAATATCGGTGATTGTAGTGATACAAGGAAGTTAGAAG
GAAATACAAATATTGTTAGATTTTCCACCATGACTCAAGAATCAGGAAGG
TTTGTAATATTTTATATAAATTTTATAATTCAATTTGTTCTTATTTTTAT
TATTATTGTTTATCATCATTATTATTATTATCCATTATTAGATAAAATTT
ATTATCACAAATTTAATTTCTTTTCGAAAATCTGCCGTTCTTAGGATATA
AAAACAATTGTATTGAGAATCCTTTATCGTTATTATTTTATAAAAAATTA
ATAATTTTTAATTTTACTTCTAACATTTACCTTTACATCCCATTTCCTTT
GAAATGTTTAAGCCATTTTATTTTTTAGCTTCAATAGACTCATTAGTCAA
TATTATTATATAGCTTCTTTCTTGATTCTTTCCTACAATCTTATCTGTTT
TCTGATTTATTTATATTATTTTTAATTATCAAAACTTCATCACCAATCAC
GAATTTTCTATCAATGTTTACTGTTATTGTTGTATTGTTATCATCAATTC
ATACTCCACTTAAGGCTTTGCTAAATATGATCTCGACTGAAGATGTCTTA
ATTGTTTCTGGTACATTCTATTTCCGGTAATAGTTCGACCCAATCTAGAT
ATTTATTATATTTTTATGATATCTGTACAGAATCTCTTATTGTTCAAAAC
TGGCGTTCAATTTGATCATTTGCCGAATGATGATACAATCTAGAAACATT
TTTCCTTCAAATACCTTACCTCTATCAACTTTTTTGATGTAAGAAGCACC
GCACTTTGACAACCAACAGTTTCTTAACTCATTATTCACATTATATTTAT
TTTGAGAAG
back to top

protein sequence of SMED30012606-orf-1

>SMED30012606-orf-1 ID=SMED30012606-orf-1|Name=SMED30012606-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=112bp
MNTNLILVTNIPKHIKLFDLIKELENKWARLIFIDNPYACLYDAFIYNPT
SLLTHFEMGVSRLRNKVINGAKPLKVYHLWFPPPNVYLTSLVIIFLIMKV
TRKSFASYLTC*
back to top

protein sequence of SMED30012606-orf-2

>SMED30012606-orf-2 ID=SMED30012606-orf-2|Name=SMED30012606-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=552bp
MLPVIFVDNLPKECDSYEIIGDLPFQTNEIKHWDIPQKEDKWMILRLPVN
QDEAIRDYVLKYIRTRRLSNGLFPRVLEVFVSEDIRLSKNMVPKTLQILP
ITTSFKSNLFFLYDLPTKLTVKAISDAAKAIGVKGSKAFFVDVVDSLRVG
FLFVPKSFPDSKFDSFQEALKEKEIVVDSNQTVMLNLNEVYLQPKGALPP
KVHRKRDDERTIEYVLTNYVYVHNVAACIDHNQLTKGSPINFKVYLFQIN
ENTTQVGFVCLRGLSDVGISQLIDHLRSLVFEGFQVMVDNIYLKCYDMPD
NNSERRAVHVPREPLDNVDNITSHVDNTFVDITEINTEESEVEVIDSDDE
DDVEILAEFSVEISSDPPPEYNLMKSDFIFVNYLPNTVEYSSFSLLPLEC
DSLTIMSCDEHKIAFLYYPDTTTDKWMGALNKLVTTKIKETNVEVSEVYL
QSDENEEMLDKGLDMISPSNYVFVYNIGDCSDTRKLEGNTNIVRFSTMTQ
ESGRFVIFYINFIIQFVLIFIIIVYHHYYYYPLLDKIYYHKFNFFSKICR
S*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 507..529