SMED30012591

Overview
NameSMED30012591
Smed IDSMED30012591
Length (bp)2518
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30012591 (SMED30012591) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30012591 (SMED30012591) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30012591 (SMED30012591) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30012591 (SMED30012591) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012591

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30012591h1SMcG0003054 SmedASXL_005654SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30012591h1SMcG0003054 SmedASXL_005654SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30012591h1SMcG0003054 dd_Smed_v6_1551_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30012591h1SMcG0003054 OX_Smed_1.0.16323ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30012591 vs. TrEMBL
Match: K1Q1K9 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10006454 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.691e-10
Identity = 85/365 (23.29%), Postives = 155/365 (42.47%), Query Frame = 1 
Query: 1156 IVALLLLIVCYIVIANAGSTSLAGHKETLGSGEGLINNIKNLVKSGKPKEMFSKISG-VLKKLGPLKMSISALISNFTSFSVKPKEESAELKAINEGFEKLNDRLDLLTMKLGNLDTLVE---ISAARAAYVQTVSTINHLYESLTDTTKDNTKESYAFFAKQYEKSYKFAAANLYDALTGSSGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDFNAKIEAKMETWKGKLDEISQRCEGLVSQCEASWKEYAYRQFTLTHDEIGNDNQVFANKLAKELDKVVPH----RSWLAVSYNDASGFDDHCYSSYYEAEKIWNKKGHDVTIFSTPKEEALTGGDLSNLVNLIE 2226
            +  LLL+  C   IA    TSL      +     L N++   ++S    + FSK +  ++  + P    I +++S   +F       SAEL AI + ++ +  R D +  +   +   ++   I A    Y  T+ TI+H Y+ L  +T  N  +S  +   Q  +SY+ A   +YD + G S  F   + +   DHVK +     EF +    L+     IE AY    + +   +     W+ KL+ I  +   +  +      +Y ++  T    +I N N +    LA E+   +      RSW+ V+  D S  D+H + ++  +  I  K   +V + S    +A       +++N ++
Sbjct:    5 VAGLLLVAFCCSPIATI-DTSL------ITDSLSLANDLGGFIES----QDFSKTAAKLISSVSPYLGVIGSVLS--FAFGFLDTGPSAELLAIQKLYKDVTLRFDKIDHQFSAVTREIKWGSIEAHFGDYESTIHTISHTYQQLVSSTSKNEFDSRKYLFTQAFRSYQKAGEKIYDGIVGQSNLFNGPIFDEAVDHVKWDRKKAQEFMLGVTKLLISAAGIEVAYYHLTNHHDSAKFIQHDWQVKLETIKSKMNAIDHKLTT---QYGHQLSTDVDIDIANHNSLSNCDLAHEVYTTIAKKYYWRSWI-VTVTDHST-DNHKFIAHACSGTINQKHSKNVVVSSVDPHKAHLTTTQQHVINYVQ 351
[Close]
BLAST of SMED30012591 vs. TrEMBL
Match: A0A0A7NXM5 (Rapunzel (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 9.463e-9
Identity = 62/286 (21.68%), Postives = 123/286 (43.01%), Query Frame = 1 
Query: 1261 INNIKNLVKSGKPKEMFSKISGVLKKLGPLKMSISALISNFTSFSVKPKEESAELKAINEGFEKLNDRLDLLTMKLGNLDTLVEISAARAAYVQ---TVSTINHLYESLTDTTKDNTKESYAFFAKQYEKSYKFAAANLYDALTGSSGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDFNAKIEAKM--ETWKGKLDEISQRCEGLVSQCEASWKEYAYRQF-TLTHDEIGNDNQVFANKLAKELDKVVPHRSWLAVSYNDASGFDDHC 2100
            +  +  L K  + K  F  ++ +    GP+  +ISA+++    F    + ES E+  + + F  LN+R+D   ++   +   ++ SA +  +      + T++ +  +L +    N +   A F   YE +YK A   L+ A+      +  NL+   + +++ +   + EF V  + LI + + IE AY+G + +N +  +K     W+ KL  +  + E + +  +  + E   +    L        N+ F              R W  + Y + SGF DH 
Sbjct:    4 VQGVVQLFKFLEEKNFFKTLTQISSVAGPIFSAISAILTVVFMF---IEIESPEMALMKKEFANLNNRIDKWGVEFQIIKRKIDWSAVQINFADIENNIITLHKMLSTLPEVPIANRETQAALFINTYEINYKAAGDILHRAMLNIEQIYSVNLLRAAQLYMENDRNQVQEFMVGMMKLITQAVQIEAAYVG-LKYNNEEASKFTQSLWEKKLAVLKDKLEQIDTDTKNKYNEQYQKDLDNLLATHYSQSNERFVTIAYDFFATKYNWREWFVLVYKELSGFKDHA 285
[Close]
BLAST of SMED30012591 vs. TrEMBL
Match: A0A3Q1APX2 (EGF-like domain-containing protein OS=Amphiprion ocellaris OX=80972 PE=4 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.644e-8
Identity = 65/295 (22.03%), Postives = 132/295 (44.75%), Query Frame = 1 
Query: 1231 KETLGSGEGLINNIKNLVKSGKPKEMFSKISGVLK---KLGPLKMSISALISNFTSFSVKPKEESAELKAINEGFEKLNDRLDLLTMKLGNLDTLVEISAARAAYVQTVSTINHLYESLTDTTKDN----TKESYAFFAKQYEKSYKFAAA-----NLYDALTGSSGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDF--NAKIEAKMETWKGKLDEISQRCEGLVSQCEASWKEYAYRQFTLTHDEIGNDNQVFANKLAKELDKVVPHRSWLAVSYN 2073
            +E + +   ++ +   ++K        S+   V+K   K+  L   I+ L+++F + ++    +   L  +  GF+++N +LD L++++ NL T VE     + Y Q    I + +   T+  + +    ++E+    A+ +   Y+ + A     NLY  LT SS +   NL NL     KC    +  F +    L+ KG+ +   Y   + F  + K +  ++ +K K+ E+       V  C+ +++EY  +        +  D    A ++ + LDK     SW+ + Y+
Sbjct:   46 REVVAASSQVVKDSLTVIKEALASAQASRAIDVIKSISKVANLAPGIAGLVASFINVALAFIPQENPLTELRRGFDEVNRKLDSLSIQISNLATDVEWFNYASVYSQDEVRILNAWNKFTEFRQSSELAQSEEAKLQLAEMFTSHYESSGAEASVSNLYHYLTVSSTALSGNLNNLLIKKFKCGVPDISRFNLYFSSLLWKGMVVSQVYWNLMGFYSSGKEDQHIQMFK-KVHEVQ---SSAVDLCQKNYEEYMKKDVVEITKRLIPDKDAIAKQVKEALDKKYDWYSWVVLVYD 336
[Close]
BLAST of SMED30012591 vs. TrEMBL
Match: A0A3P8SHY1 (EGF-like domain-containing protein OS=Amphiprion percula OX=161767 PE=4 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.645e-8
Identity = 65/295 (22.03%), Postives = 132/295 (44.75%), Query Frame = 1 
Query: 1231 KETLGSGEGLINNIKNLVKSGKPKEMFSKISGVLK---KLGPLKMSISALISNFTSFSVKPKEESAELKAINEGFEKLNDRLDLLTMKLGNLDTLVEISAARAAYVQTVSTINHLYESLTDTTKDN----TKESYAFFAKQYEKSYKFAAA-----NLYDALTGSSGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDF--NAKIEAKMETWKGKLDEISQRCEGLVSQCEASWKEYAYRQFTLTHDEIGNDNQVFANKLAKELDKVVPHRSWLAVSYN 2073
            +E + +   ++ +   ++K        S+   V+K   K+  L   I+ L+++F + ++    +   L  +  GF+++N +LD L++++ NL T VE     + Y Q    I + +   T+  + +    ++E+    A+ +   Y+ + A     NLY  LT SS +   NL NL     KC    +  F +    L+ KG+ +   Y   + F  + K +  ++ +K K+ E+       V  C+ +++EY  +        +  D    A ++ + LDK     SW+ + Y+
Sbjct:   46 REVVAASSQVVKDSLTVIKEALASAQASRAIDVIKSISKVANLAPGIAGLVASFINVALAFIPQENPLTELRRGFDEVNRKLDSLSIQISNLATDVEWFNYASVYSQDEVRILNAWNKFTEFRQSSELAQSEEAKLQLAEMFTSHYESSGAEASVSNLYHYLTVSSTALSGNLNNLLIKKFKCGVPDISRFNLYFSSLLWKGMVVSQVYWNLMGFYSSGKEDQHIQMFK-KVHEVQ---SSAVDLCQKNYEEYMKKDVVEITKRLIPDKDAIAKQVKEALDKKYDWYSWVVLVYD 336
[Close]
BLAST of SMED30012591 vs. TrEMBL
Match: A0A1I8HCC0 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 2.375e-8
Identity = 64/273 (23.44%), Postives = 121/273 (44.32%), Query Frame = 1 
Query: 1408 EESAELKAINEGFEKLNDRLDLLTMKLGNLDTLVEISAARAAYVQTVSTI---NHLYESLTDTTKDNTKESYAFFAKQYEKSYK---FAAANLYDALTGS----SGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDFNAKIEAKMETWKGKLDEISQRCEGLVSQCEASWKEYAYRQFTLTHDEIGNDNQVFANK---------LAKELDKVVPHRSWLAVSYNDASGFDDHCYSSYYEAEKIWNKKGHDVTIFST 2169
            +ESAE+K + E F++++ +LD ++++L  +  L+E    R  Y    S I    HL +S        T+E +    +Q+  +++    AA  LY A+  +    S + G  +++  ++H +    ++M F +    L+ +G  +E AY   +  +         W  +++++  R    + Q E       + Q T     +  DN+  +NK         LA + D+    R W+A+SY D SG D H  S      +     G +V + S+
Sbjct: 2295 QESAEMKFMREQFKEVHAKLDSISLQLEEVKRLIEWQTTRVTYANYESFIVATTHLLDSYVGANDAMTREVH---KRQFLTAFRKNPIAALKLYQAVVETDLTISDNLGVAVVHYTQNHRR----NVMNFLLAMQRLVMRGAQLEIAYEKMVHGDHAATVTQALWDTRMEKMLTR----MQQVENGVLSKYHSQMTSDIGRVILDNRHLSNKDFSGHMYRFLADKFDR----RYWIALSYKDVSGGDKHWVSYCGGDARFRTHGGRNVVVASS 2552

HSP 2 Score: 62.7734 bits (151), Expect = 4.319e-6
Identity = 58/283 (20.49%), Postives = 124/283 (43.82%), Query Frame = 1 
Query: 1303 EMFSKISGVLKKLGPLKMSISALISNFTSFSVKPK-EESAELKAINEGFEKLNDRLDLLTMKLGNLDTLVEISAARAAYVQ------TVSTINHLYESLTDTTKDNTKESYAFFAKQYEKSYKFAAANLYDALTGSSGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDFNAKIEAKMETWKGKLDEISQRCEGLVSQCEASWKEYAYRQFTLTHDEIGN--------DNQVFANKLAKELDKVVPHRSWLAVSYNDASGFDDHCYS 2106
            E+F K++G + ++  +   +   +++        +  ESAE+K + E F +++ +LD ++++L ++  L+E    R  Y          S+I + Y +  +++++  K  +   A Q+      A   LY A+         NL      + + +   ++ F +    LI +G  +E AY   +  +         W G+++++  R   L ++ ++ +    + Q T    +IG          N+ F+  + + L     HR+W +++Y D SG D H  S
Sbjct: 1512 EIFEKVNGNVTQVAEVVSKVFTFVTSVIDLIQHLQGTESAEMKFLREQFAQVHAKLDSISLQLDDVKRLIEWRTTRVMYANYETFIIAASSILNNYVAAPESSREVLKRQF-IAAFQHN---PLAPLKLYQAIVEKDFVIAENLAAAVVHYTQNHRRHVINFLLAMQRLIMRGAQLEIAYEKMVHGDGAGNVAKTVWNGRMEKLLIRMMRLENEIKSKY----HSQMT---SDIGGVILNNRHLSNKDFSGHMYRFLADKFDHRNWFSLAYKDVSGGDKHWVS 1783
[Close]
BLAST of SMED30012591 vs. Ensembl Medaka
Match: rpz2 (uncharacterized LOC101161776 [Source:NCBI gene;Acc:101161776])

HSP 1 Score: 50.8322 bits (120), Expect = 1.754e-6
Identity = 47/215 (21.86%), Postives = 95/215 (44.19%), Query Frame = 1 
Query: 1315 KISGVLKKLGPLKMSISALISNFTSFSVKPKEESAELKAINEGFEKLNDRLDLLTMKLGNLDTLVEISAARAAYVQTVSTINHLYESLTDTTK--DNTKESYAFFAKQYEKSYKFAAANL-----YDALTGSSGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDFNAKIEAKM-ETWKGKLDEISQRCEGLVSQCEAS 1935
            K+S     + P+   +S+L+       VK      E   +++ F++++ +LD ++ K       + I+  +  Y      I H Y+S  D         E+   + K++E  Y+   A++     Y  + G   +FG  L+ +Y D+   +  ++   C    HL + GL    A +G+       E ++ E W  ++ +I ++ E ++SQC +S
Sbjct:   20 KVSSFASSIDPIFGIVSSLVGVARKGLVKD-----EGHPLDKDFQEISSKLDTISRKNQECLKKILINEVKETYGTYEKYIRHQYQSFLDMVAKVKTDPENATAYMKKFESIYERDKADMSLDVFYRGVMGKGMTFGRPLLKVYLDNCDGDRDTMERHCSYIAHLFHMGLI---ALMGYTAVTEDDEDEVREKWAKRVLDIQEKMEEVLSQCSSS 226
[Close]
BLAST of SMED30012591 vs. Planmine SMEST
Match: SMESG000046431.1 (SMESG000046431.1)

HSP 1 Score: 930.628 bits (2404), Expect = 0.000e+0
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 1 
Query: 1126 MRIFIKSTEQIVALLLLIVCYIVIANAGSTSLAGHKETLGSGEGLINNIKNLVKSGKPKEMFSKISGVLKKLGPLKMSISALISNFTSFSVKPKEESAELKAINEGFEKLNDRLDLLTMKLGNLDTLVEISAARAAYVQTVSTINHLYESLTDTTKDNTKESYAFFAKQYEKSYKFAAANLYDALTGSSGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDFNAKIEAKMETWKGKLDEISQRCEGLVSQCEASWKEYAYRQFTLTHDEIGNDNQVFANKLAKELDKVVPHRSWLAVSYNDASGFDDHCYSSYYEAEKIWNKKGHDVTIFSTPKEEALTGGDLSNLVNLIEAQKQQVLKLQNCNGKYYWLVRHKRGIEAARAIADKIRDNYYRGTVYTINMRFSPGYSATGNTLYATEQKFHCGSETYKVLIFQ 2475
            MRIFIKSTEQIVALLLLIVCYIVIANAGSTSLAGHKETLGSGEGLINNIKNLVKSGKPKEMFSKISGVLKKLGPLKMSISALISNFTSFSVKPKEESAELKAINEGFEKLNDRLDLLTMKLGNLDTLVEISAARAAYVQTVSTINHLYESLTDTTKDNTKESYAFFAKQYEKSYKFAAANLYDALTGSSGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDFNAKIEAKMETWKGKLDEISQRCEGLVSQCEASWKEYAYRQFTLTHDEIGNDNQVFANKLAKELDKVVPHRSWLAVSYNDASGFDDHCYSSYYEAEKIWNKKGHDVTIFSTPKEEALTGGDLSNLVNLIEAQKQQVLKLQNCNGKYYWLVRHKRGIEAARAIADKIRDNYYRGTVYTINMRFSPGYSATGNTLYATEQKFHCGSETYKVLIFQ
Sbjct:    1 MRIFIKSTEQIVALLLLIVCYIVIANAGSTSLAGHKETLGSGEGLINNIKNLVKSGKPKEMFSKISGVLKKLGPLKMSISALISNFTSFSVKPKEESAELKAINEGFEKLNDRLDLLTMKLGNLDTLVEISAARAAYVQTVSTINHLYESLTDTTKDNTKESYAFFAKQYEKSYKFAAANLYDALTGSSGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDFNAKIEAKMETWKGKLDEISQRCEGLVSQCEASWKEYAYRQFTLTHDEIGNDNQVFANKLAKELDKVVPHRSWLAVSYNDASGFDDHCYSSYYEAEKIWNKKGHDVTIFSTPKEEALTGGDLSNLVNLIEAQKQQVLKLQNCNGKYYWLVRHKRGIEAARAIADKIRDNYYRGTVYTINMRFSPGYSATGNTLYATEQKFHCGSETYKVLIFQ 450
[Close]
BLAST of SMED30012591 vs. Planmine SMEST
Match: SMESG000027653.1 (SMESG000027653.1)

HSP 1 Score: 930.628 bits (2404), Expect = 0.000e+0
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 1 
Query: 1126 MRIFIKSTEQIVALLLLIVCYIVIANAGSTSLAGHKETLGSGEGLINNIKNLVKSGKPKEMFSKISGVLKKLGPLKMSISALISNFTSFSVKPKEESAELKAINEGFEKLNDRLDLLTMKLGNLDTLVEISAARAAYVQTVSTINHLYESLTDTTKDNTKESYAFFAKQYEKSYKFAAANLYDALTGSSGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDFNAKIEAKMETWKGKLDEISQRCEGLVSQCEASWKEYAYRQFTLTHDEIGNDNQVFANKLAKELDKVVPHRSWLAVSYNDASGFDDHCYSSYYEAEKIWNKKGHDVTIFSTPKEEALTGGDLSNLVNLIEAQKQQVLKLQNCNGKYYWLVRHKRGIEAARAIADKIRDNYYRGTVYTINMRFSPGYSATGNTLYATEQKFHCGSETYKVLIFQ 2475
            MRIFIKSTEQIVALLLLIVCYIVIANAGSTSLAGHKETLGSGEGLINNIKNLVKSGKPKEMFSKISGVLKKLGPLKMSISALISNFTSFSVKPKEESAELKAINEGFEKLNDRLDLLTMKLGNLDTLVEISAARAAYVQTVSTINHLYESLTDTTKDNTKESYAFFAKQYEKSYKFAAANLYDALTGSSGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDFNAKIEAKMETWKGKLDEISQRCEGLVSQCEASWKEYAYRQFTLTHDEIGNDNQVFANKLAKELDKVVPHRSWLAVSYNDASGFDDHCYSSYYEAEKIWNKKGHDVTIFSTPKEEALTGGDLSNLVNLIEAQKQQVLKLQNCNGKYYWLVRHKRGIEAARAIADKIRDNYYRGTVYTINMRFSPGYSATGNTLYATEQKFHCGSETYKVLIFQ
Sbjct:    1 MRIFIKSTEQIVALLLLIVCYIVIANAGSTSLAGHKETLGSGEGLINNIKNLVKSGKPKEMFSKISGVLKKLGPLKMSISALISNFTSFSVKPKEESAELKAINEGFEKLNDRLDLLTMKLGNLDTLVEISAARAAYVQTVSTINHLYESLTDTTKDNTKESYAFFAKQYEKSYKFAAANLYDALTGSSGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDFNAKIEAKMETWKGKLDEISQRCEGLVSQCEASWKEYAYRQFTLTHDEIGNDNQVFANKLAKELDKVVPHRSWLAVSYNDASGFDDHCYSSYYEAEKIWNKKGHDVTIFSTPKEEALTGGDLSNLVNLIEAQKQQVLKLQNCNGKYYWLVRHKRGIEAARAIADKIRDNYYRGTVYTINMRFSPGYSATGNTLYATEQKFHCGSETYKVLIFQ 450
[Close]
BLAST of SMED30012591 vs. Planmine SMEST
Match: SMESG000027656.1 (SMESG000027656.1)

HSP 1 Score: 480.33 bits (1235), Expect = 1.952e-162
Identity = 215/443 (48.53%), Postives = 313/443 (70.65%), Query Frame = 1 
Query: 1165 LLLLIVCY-----IVIANAGSTSLAGHKETLGSGEGLINNIKNLVKSGKPKEMFSKISGVLKKLGPLKMSISALISNFTSFSVKPKEESAELKAINEGFEKLNDRLDLLTMKLGNLDTLVEISAARAAYVQTVSTINHLYESLTDTTKDNTKESYAFFAKQYEKSYKFAAANLYDALTGSSGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDFNAKIEAKMETWKGKLDEISQRCEGLVSQCEASWKEYAYRQFTLTHDEIGNDNQVFANKLAKELDKVVPHRSWLAVSYNDASGFDDHCYSSYYEAEKIWNKKGHDVTIFSTPKEEALTGGDLSNLVNLIEAQKQQVLKLQNCNGKYYWLVRHKR-GIEAARAIADKIRDNYYRGTVYTINMRFSPGYSATGNTLYATEQKFHCGSETYKVLIFQ 2475
            + L I C      ++  +  + ++   +  L S + +  N+   + S  P ++F+K+SGVL  LGP  M+ISA++S  T F   PKE+S ELKAI EGFEK+N++LD ++ K+G+L+ ++E+S+ARAAYV+TV++IN+ YE+L +  K N+KE Y +FAKQYE S++FA AN+YDA+TGS+G FGFN++NLYK HV+CN V L +FC MG+ LI KGL++EGAYLG+++F+AK + K ETW  K++E+S++C  ++  CE++WKE+AY+Q+  TH E+  DNQ FA +LAK+L+ V P RSWL ++YNDASGF  H YS+YYE   IWN++ HD ++FS PK   LTG D  N V LI  QK+ + ++ +C    +WL++  R   E AR I +KIR+ Y  G VY I + + PG +ATGNTLYATEQK+ C  E ++++IFQ
Sbjct:    6 VFLFIACLFSLFSVIKCSYEADTIKKTQAVLASSKDVFANLSEFITSANPGKLFAKLSGVLGSLGPAAMAISAILSIVTMFL--PKEDSPELKAIKEGFEKVNNKLDTISSKIGSLENIIELSSARAAYVKTVTSINNNYETLNEAKKSNSKEGYDYFAKQYENSFEFATANMYDAMTGSTGPFGFNMVNLYKTHVRCNPVKLSQFCTMGLQLITKGLTVEGAYLGYMNFSAKAKGKTETWTTKINEVSKQCSAVIEACESNWKEFAYQQYKETHRELEGDNQAFAERLAKDLENVAPQRSWLTIAYNDASGFGPHTYSTYYEVHTIWNERNHDTSVFSVPKSPKLTGSDYDNFVKLINGQKEAIARVDDCKRTEFWLIKKDRYENEDARTIVNKIRERYMTGVVYAIRLDYKPGLAATGNTLYATEQKWQCTGEQFRLIIFQ 446
[Close]
BLAST of SMED30012591 vs. Planmine SMEST
Match: SMESG000037569.1 (SMESG000037569.1)

HSP 1 Score: 459.529 bits (1181), Expect = 1.984e-154
Identity = 217/450 (48.22%), Postives = 317/450 (70.44%), Query Frame = 1 
Query: 1126 MRIFIK-STEQIVALLLLIVCYIVIANAGSTSLAGHKETLGSGEGLINNIKNLVKSGKPKEMFSKISGVLKKLGPLKMSISALISNFTSFSVKPKEESAELKAINEGFEKLNDRLDLLTMKLGNLDTLVEISAARAAYVQTVSTINHLYESLTDTTKDNTKESYAFFAKQYEKSYKFAAANLYDALTGSSGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDFNAKIEAKMETWKGKLDEISQRCEGLVSQCEASWKEYAYRQFTLTHDEIGNDNQVFANKLAKELDKVVPHRSWLAVSYNDASGFDDHCYSSYYEAEKIWNKKGHDVTIFSTPKEEALTGGDLSNLVNLIEAQKQQVLKLQNCNGKYYWLVRHKRGIEAARAIADKIRDNYYRGTVYTINMRFSPGYSATGNTLYATEQKFHCGSETYKVLIF 2472
            M +F K  T  ++A+ L+ +  ++  N    S+   +  L S + + +N+  ++++  P ++F+K+S VL  LGP  M+ISA++S  + F   PKE+S ELKAI  GFEK+N++LD++T K+GNL+ ++E+S+ARAAYV+TV+ IN+ Y++L D  ++NTKE Y FFAKQYE S++FA  N+YDA+TGS+G FGFN++NLYK +VKCN V L +FC MGI LINKGL+IEGAY+G+++F++K EAK E+WK K+D + ++C  L+  CE SWK+Y Y+QF  T+ E+G DNQ FA K+A++L+ VVP RSWLAV YNDASGF +H YSSYYE EK+W++K HDVTIFSTP++E LTG +L NL+  I  QK ++     C           R  EA R + + IR+ +  G VY +     PG++ATGN +  ++QK+ C + TY+++IF
Sbjct:    1 MNVFNKLETFVLIAICLVSLVNVINCNFADESIKKSQAVLASSKEVFSNLNEVIQAINPGKLFAKLSTVLGALGPAAMAISAVLSIVSMFL--PKEDSPELKAIKAGFEKVNNKLDIITSKIGNLENIIELSSARAAYVKTVTNINNNYKALEDAQRENTKEGYQFFAKQYENSFEFATENIYDAMTGSTGPFGFNMVNLYKTNVKCNPVRLSQFCTMGISLINKGLTIEGAYIGYLNFSSKAEAKAESWKVKIDALMKQCSSLIESCENSWKDYGYQQFKDTYRELGGDNQKFAEKIAQDLNNVVPQRSWLAVVYNDASGFGEHTYSSYYETEKVWDQKDHDVTIFSTPRQEELTGSNLENLIKPINEQKNKIFNTNECGSS------RLRNNEARRQV-ETIREKFSGGVVYAVFGDKRPGHAATGNNMIVSDQKWKCNNYTYRIIIF 441
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BLAST of SMED30012591 vs. Planmine SMEST
Match: SMESG000062792.1 (SMESG000062792.1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.490e-14
Identity = 72/304 (23.68%), Postives = 128/304 (42.11%), Query Frame = 1 
Query: 1309 FSKISGVLKKLG-PLKMSI----SALISNFTSFSVKPKEESAELKAINEGFEKLNDRLDLLTMKLGNLDTLVEISAARAAYVQTVSTINHLYE---SLTDTTKDNTKESYAFFAKQYEKSYKFAAANLYDALTGSSGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDFNAKIEAKMETWKGKLDEISQRCEGLVSQCEASWKEYAYRQFTLTHDEI-----GNDNQVFANKLAKELDKVVPHRSWLAVSYNDASGFDDHCYSSYYEAEKIWNKKGHDVTIFSTPKEE 2181
            F K +  + K G PL  SI      +++ F +       ES EL  + E F+ +ND++D +  +   +   +  +  +  Y    + I  L +   S++D   +  +     F   Y+++Y  AA  LY++ T     F  NL+         +   + +F +  I LI KGL +E  YL   +  +  +   E W  K+++IS   E  ++  + +W    + Q     +E      G  N+ +  +    L      R W  V YN  +G D+H Y S Y        +GH++ + S  KE+
Sbjct:   50 FVKTTQAISKWGLPLLKSIGYFSKVIMAQFET-------ESKELTYMKEQFKIINDKMDTVDQQFDEVKKAINWNVVKVQYFNHETAIGLLQDMILSISDAPSNVRQMMMDRFRITYDQTYYNAADKLYESFTNPQRIFSENLVTASCQTFNFHRRKVEKFFLGNIQLILKGLEVESFYLTTKNLTSNQDYWAEQWNKKINQISAIMEETINSIKGNW----HSQLQKDAEEFNVKNYGKGNKEYLEEFYNFLTDKFSWREWFLVVYNPITGGDNH-YVSVYGGIINLRSRGHNIAVASRDKED 341
The following BLAST results are available for this feature:
BLAST of SMED30012591 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012591 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012591 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012591 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012591 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012591 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012591 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012591 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
K1Q1K99.691e-1023.29Uncharacterized protein OS=Crassostrea gigas OX=29... [more]
A0A0A7NXM59.463e-921.68Rapunzel (Fragment) OS=Schmidtea mediterranea OX=7... [more]
A0A3Q1APX21.644e-822.03EGF-like domain-containing protein OS=Amphiprion o... [more]
A0A3P8SHY11.645e-822.03EGF-like domain-containing protein OS=Amphiprion p... [more]
A0A1I8HCC02.375e-823.44MFS domain-containing protein OS=Macrostomum ligna... [more]
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BLAST of SMED30012591 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012591 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012591 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012591 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012591 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpz21.754e-621.86uncharacterized LOC101161776 [Source:NCBI gene;Acc... [more]
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BLAST of SMED30012591 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000046431.10.000e+0100.00SMESG000046431.1[more]
SMESG000027653.10.000e+0100.00SMESG000027653.1[more]
SMESG000027656.11.952e-16248.53SMESG000027656.1[more]
SMESG000037569.11.984e-15448.22SMESG000037569.1[more]
SMESG000062792.11.490e-1423.68SMESG000062792.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012591 ID=SMED30012591|Name=SMED30012591|organism=Schmidtea mediterranea sexual|type=transcript|length=2518bp
GTAAATAGAATAAATACAGATCAAAATAATTTACAATTGATAAATAACAA
ATTAACTCATACTGTCATGATACATACTGATGAACAATAAATTGTTAATT
TAACAAAATCAAAACAAAACTTAAATTCAAAATCATCTAATAATCCAAGA
ACAATTATGCTAATATGGTAAAATATGAAGTTTGAAAATTAATGGAAAAA
ATAACTCATGTTTTCATAATATCAATAAAAATTAACTACCAAAAAAAAAT
TTAATCGCCTTACAATTCATAACTTCCGCAATCAAAGATTGACAAAATGT
GGAACAGTCACAATTATATAATGTAGATAACAATAATAATAGTAAATAAA
AGTAAATAATAATCAATAATTAAAAAAACTAATAAATAAATAGCATTAAT
ATAAATAATGATAAATATAATATCGATAAAAGAATCAGCGGACAATAGTA
AAATAAAAAATCTACCAATAAATAATGTTGTATTTGAAACACCTCATACA
AATGTGACAGAAAAAAACAACAACGAAGCATAAATAGTTTTTAAGATAAT
AATCTGAACCAAAGAACCAAATTTGCAGCTCATCATTGACAACTCTATTC
AAACAACACATTGATTTCAGAATAATAATTCTTACATTTATATTTATAAG
AAAGTGGAATGATAAAAATAATAAGATTGCCAAAATAATGAAAAACGCAA
CAATATTGAATAGTTACGACTACAACAAATGTAATGCTCAGTAGTGGGTG
TTAATATTATTATTATTATAACTATCATTAATCATAACAATAAAATAATA
ATAATAGCATAATTATCATTAATAATTAACGACGCAATACGCATTTGCCA
GCATAATACGTATCAAATTGTAATTAATTGGCAAATGACACAGTTCATCA
TTTTCGAAAAAAGAGTTCTCATAATAGAACACAAAAAATGATTAAAAATC
ATTGAAACAAGATAAACAGCGTAGTCACTTCCACCAAATATATTTAGTTA
TTATTATTATTATTATTATTTTAGCACTGTTGAAATGTTGATCGAGATAT
AAAAGGCAAAACAGAACTAAGTAAATATGAATTAGATTTTTGTATAAAAG
TCATAGTAAAAATATAAAAATAAAAATGAGAATTTTCATCAAATCAACAG
AACAAATTGTTGCTTTGCTTTTATTAATCGTGTGCTATATCGTAATTGCA
AATGCTGGAAGCACTAGTTTAGCCGGTCATAAGGAGACTCTGGGATCTGG
AGAAGGACTGATAAACAACATCAAGAATCTAGTGAAATCAGGAAAACCCA
AAGAAATGTTTAGTAAAATTAGCGGTGTTTTGAAAAAATTGGGACCTTTG
AAAATGTCTATTTCTGCTTTAATAAGCAATTTTACGTCGTTTTCAGTTAA
ACCAAAGGAAGAAAGTGCTGAGCTTAAAGCAATTAATGAAGGTTTTGAGA
AATTAAACGACCGATTGGATCTTTTGACAATGAAACTAGGAAATCTCGAT
ACCTTAGTTGAAATAAGTGCCGCAAGAGCAGCCTATGTTCAAACTGTATC
GACAATAAATCATTTATACGAATCCTTGACAGATACTACTAAAGATAACA
CCAAAGAATCGTATGCGTTCTTTGCGAAACAATATGAAAAAAGCTACAAA
TTTGCAGCAGCAAACTTGTATGATGCATTAACTGGTTCATCTGGAAGTTT
TGGTTTTAATTTAATAAATTTGTACAAAGATCATGTCAAATGTAATACAG
TCAGTCTTATGGAATTCTGTGTAATGGGAATTCATTTAATCAACAAAGGA
CTGTCGATAGAAGGTGCTTATTTGGGATTCATTGATTTTAATGCCAAAAT
TGAAGCAAAAATGGAAACTTGGAAAGGTAAACTCGATGAAATATCTCAAA
GATGTGAAGGATTAGTATCACAGTGTGAAGCGAGTTGGAAAGAGTATGCC
TATCGACAGTTTACACTAACTCACGACGAGATCGGCAACGACAATCAAGT
TTTTGCTAATAAATTAGCTAAGGAATTGGATAAAGTTGTACCTCACAGAA
GCTGGTTAGCTGTATCTTATAATGATGCAAGCGGTTTCGATGATCACTGC
TATTCTAGTTACTACGAAGCTGAAAAGATTTGGAACAAAAAAGGACATGA
TGTTACAATATTTTCAACACCGAAAGAAGAAGCATTAACAGGAGGTGACC
TAAGTAACTTAGTAAATTTGATAGAAGCACAAAAGCAACAAGTTTTAAAA
TTACAAAATTGCAATGGTAAATACTATTGGCTGGTGAGACACAAAAGGGG
TATTGAAGCTGCAAGAGCCATCGCAGATAAAATTCGAGATAACTACTATC
GGGGAACTGTCTACACAATTAATATGAGGTTCAGCCCAGGATATTCTGCA
ACGGGGAATACACTTTATGCAACGGAACAGAAATTTCATTGCGGATCTGA
AACATACAAAGTACTTATATTCCAGTGATTCAATAATAGATTTTGAGGAA
TAAATTCAACAATTGATT
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protein sequence of SMED30012591-orf-1

>SMED30012591-orf-1 ID=SMED30012591-orf-1|Name=SMED30012591-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=451bp
MRIFIKSTEQIVALLLLIVCYIVIANAGSTSLAGHKETLGSGEGLINNIK
NLVKSGKPKEMFSKISGVLKKLGPLKMSISALISNFTSFSVKPKEESAEL
KAINEGFEKLNDRLDLLTMKLGNLDTLVEISAARAAYVQTVSTINHLYES
LTDTTKDNTKESYAFFAKQYEKSYKFAAANLYDALTGSSGSFGFNLINLY
KDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGFIDFNAKIEAKMETWK
GKLDEISQRCEGLVSQCEASWKEYAYRQFTLTHDEIGNDNQVFANKLAKE
LDKVVPHRSWLAVSYNDASGFDDHCYSSYYEAEKIWNKKGHDVTIFSTPK
EEALTGGDLSNLVNLIEAQKQQVLKLQNCNGKYYWLVRHKRGIEAARAIA
DKIRDNYYRGTVYTINMRFSPGYSATGNTLYATEQKFHCGSETYKVLIFQ
*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0000418head
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR039051SE-CTX-like
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 239..259
NoneNo IPR availableTMHMMTMhelixcoord: 12..34
IPR039051SE-cephalotoxin-likePANTHERPTHR40472FAMILY NOT NAMEDcoord: 65..378