Leucine--tRNA ligase
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30012572 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 12
Homology
BLAST of Leucine--tRNA ligase vs. Ensembl Human
Match: LARS2 (leucyl-tRNA synthetase 2, mitochondrial [Source:HGNC Symbol;Acc:HGNC:17095]) HSP 1 Score: 497.278 bits (1279), Expect = 8.726e-162 Identity = 296/795 (37.23%), Postives = 441/795 (55.47%), Query Frame = 2
Query: 200 YDFNVKKQLETFW----QEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDL-VDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIKHPLSGRLIP------------IIRNQNL------KFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTL----RIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQ-ENDIVLGIQNWERRVWNLIKEIVNSPVN--DPKVFLVNDFLLQDSEFIAKYQ-SLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFXXXXXXXXXXXXXXXWAQLA 2491
Y +K +E +W +E+ SKI++ D ++ K Y+LSMFPYPSG+LHMGH+RVYT +D +AR ++ +VI PMGWDAFGLPAENAA++R P+ WT NI M++Q+ +++ L W RE++TC P+YYKWTQ+LF +L+E G YQK +VNWDP+D+TVLA+E +D G SWRSGA VE+ L+QWFI+TTA ++++ D L + +W + Q WIGD G ++DF L V Q EKL+ +T P A + V I+ + L H SL K L L+P ++ Q + K + + S++G+P +D LA LG+ AY+ ++++ +E + + L R +GG S DWLISRQRYWGTPIPIVHCP CGP P LPV LP + + PL+ A W+ CP+C A RE+DTMDTFVDS+WY+ RY D N + +V MPVD+YIGG EHA+ HL+YARF SHF H D K+ EPF + L GL+ QT++ SG+++ +V PV+ +E L W+KMSKSK NG+ P+ VV++YG+D RL IL P+K+ W + D + G+ W++R+W L + + + P+ L+++ ++ + +Y+ S+++++ + + F+++ +S L L++ + S S + L ++ PL+P ++SE+WA LA
Sbjct: 51 YTLQTRKDVEKWWHQRIKEQASKISEADK-SKPKFYVLSMFPYPSGKLHMGHVRVYTISDTIARFQKMRGMQVINPMGWDAFGLPAENAAVERNLHPQSWTQSNIKHMRKQL-DRLGLCFSWDREITTCLPDYYKWTQYLFIKLYEAGLAYQKEALVNWDPVDQTVLANEQVDEHGCSWRSGAKVEQKYLRQWFIKTTAYAKAMQDALADLP--EWYGIKGMQAHWIGDCVGCHLDFTLKVHGQATG---EKLTAYTATPEAIYG--TSHVAISPSH-------------RLLHGHSSL--KEALRMALVPGKDCLTPVMAVNMLTQQEVPVVILAKADLEGSLDSKIGIPSTSSEDTILAQTLGL--------AYSEVIETLPDGTERLSSSAEFTGMTRQDAFLALTQKARGKRVGGDVTSDKLKDWLISRQRYWGTPIPIVHCPVCGPTPVPLEDLPVTLP-----NIASFTGKGGPPLAMASEWVNCSCPRCKG-AAKRETDTMDTFVDSAWYYFRYTDPHNPHSPFNTAVADYWMPVDLYIGGKEHAVMHLFYARFFSHFCH---DQKMVKHREPFHKLLAQGLIKGQTFRLPSGQYLQREEVDLTGSVPVHAKTKEKLEVTWEKMSKSKHNGVDPEEVVEQYGIDTIRLYILFAAPPEKDILWDVKTDALPGVLRWQQRLWTLTTRFIEARASGKSPQPQLLSNKEKAEARKLWEYKNSVISQVTTHFTEDFSLNSAISQLMGLSNALSQASQSVILHSPEFEDALCALMVMAAPLAPHVTSEIWAGLA 804
BLAST of Leucine--tRNA ligase vs. Ensembl Human
Match: LARS2 (leucyl-tRNA synthetase 2, mitochondrial [Source:HGNC Symbol;Acc:HGNC:17095]) HSP 1 Score: 497.278 bits (1279), Expect = 8.726e-162 Identity = 296/795 (37.23%), Postives = 441/795 (55.47%), Query Frame = 2
Query: 200 YDFNVKKQLETFW----QEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDL-VDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIKHPLSGRLIP------------IIRNQNL------KFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTL----RIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQ-ENDIVLGIQNWERRVWNLIKEIVNSPVN--DPKVFLVNDFLLQDSEFIAKYQ-SLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFXXXXXXXXXXXXXXXWAQLA 2491
Y +K +E +W +E+ SKI++ D ++ K Y+LSMFPYPSG+LHMGH+RVYT +D +AR ++ +VI PMGWDAFGLPAENAA++R P+ WT NI M++Q+ +++ L W RE++TC P+YYKWTQ+LF +L+E G YQK +VNWDP+D+TVLA+E +D G SWRSGA VE+ L+QWFI+TTA ++++ D L + +W + Q WIGD G ++DF L V Q EKL+ +T P A + V I+ + L H SL K L L+P ++ Q + K + + S++G+P +D LA LG+ AY+ ++++ +E + + L R +GG S DWLISRQRYWGTPIPIVHCP CGP P LPV LP + + PL+ A W+ CP+C A RE+DTMDTFVDS+WY+ RY D N + +V MPVD+YIGG EHA+ HL+YARF SHF H D K+ EPF + L GL+ QT++ SG+++ +V PV+ +E L W+KMSKSK NG+ P+ VV++YG+D RL IL P+K+ W + D + G+ W++R+W L + + + P+ L+++ ++ + +Y+ S+++++ + + F+++ +S L L++ + S S + L ++ PL+P ++SE+WA LA
Sbjct: 51 YTLQTRKDVEKWWHQRIKEQASKISEADK-SKPKFYVLSMFPYPSGKLHMGHVRVYTISDTIARFQKMRGMQVINPMGWDAFGLPAENAAVERNLHPQSWTQSNIKHMRKQL-DRLGLCFSWDREITTCLPDYYKWTQYLFIKLYEAGLAYQKEALVNWDPVDQTVLANEQVDEHGCSWRSGAKVEQKYLRQWFIKTTAYAKAMQDALADLP--EWYGIKGMQAHWIGDCVGCHLDFTLKVHGQATG---EKLTAYTATPEAIYG--TSHVAISPSH-------------RLLHGHSSL--KEALRMALVPGKDCLTPVMAVNMLTQQEVPVVILAKADLEGSLDSKIGIPSTSSEDTILAQTLGL--------AYSEVIETLPDGTERLSSSAEFTGMTRQDAFLALTQKARGKRVGGDVTSDKLKDWLISRQRYWGTPIPIVHCPVCGPTPVPLEDLPVTLP-----NIASFTGKGGPPLAMASEWVNCSCPRCKG-AAKRETDTMDTFVDSAWYYFRYTDPHNPHSPFNTAVADYWMPVDLYIGGKEHAVMHLFYARFFSHFCH---DQKMVKHREPFHKLLAQGLIKGQTFRLPSGQYLQREEVDLTGSVPVHAKTKEKLEVTWEKMSKSKHNGVDPEEVVEQYGIDTIRLYILFAAPPEKDILWDVKTDALPGVLRWQQRLWTLTTRFIEARASGKSPQPQLLSNKEKAEARKLWEYKNSVISQVTTHFTEDFSLNSAISQLMGLSNALSQASQSVILHSPEFEDALCALMVMAAPLAPHVTSEIWAGLA 804
BLAST of Leucine--tRNA ligase vs. Ensembl Human
Match: LARS2 (leucyl-tRNA synthetase 2, mitochondrial [Source:HGNC Symbol;Acc:HGNC:17095]) HSP 1 Score: 497.278 bits (1279), Expect = 8.726e-162 Identity = 296/795 (37.23%), Postives = 441/795 (55.47%), Query Frame = 2
Query: 200 YDFNVKKQLETFW----QEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDL-VDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIKHPLSGRLIP------------IIRNQNL------KFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTL----RIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQ-ENDIVLGIQNWERRVWNLIKEIVNSPVN--DPKVFLVNDFLLQDSEFIAKYQ-SLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFXXXXXXXXXXXXXXXWAQLA 2491
Y +K +E +W +E+ SKI++ D ++ K Y+LSMFPYPSG+LHMGH+RVYT +D +AR ++ +VI PMGWDAFGLPAENAA++R P+ WT NI M++Q+ +++ L W RE++TC P+YYKWTQ+LF +L+E G YQK +VNWDP+D+TVLA+E +D G SWRSGA VE+ L+QWFI+TTA ++++ D L + +W + Q WIGD G ++DF L V Q EKL+ +T P A + V I+ + L H SL K L L+P ++ Q + K + + S++G+P +D LA LG+ AY+ ++++ +E + + L R +GG S DWLISRQRYWGTPIPIVHCP CGP P LPV LP + + PL+ A W+ CP+C A RE+DTMDTFVDS+WY+ RY D N + +V MPVD+YIGG EHA+ HL+YARF SHF H D K+ EPF + L GL+ QT++ SG+++ +V PV+ +E L W+KMSKSK NG+ P+ VV++YG+D RL IL P+K+ W + D + G+ W++R+W L + + + P+ L+++ ++ + +Y+ S+++++ + + F+++ +S L L++ + S S + L ++ PL+P ++SE+WA LA
Sbjct: 51 YTLQTRKDVEKWWHQRIKEQASKISEADK-SKPKFYVLSMFPYPSGKLHMGHVRVYTISDTIARFQKMRGMQVINPMGWDAFGLPAENAAVERNLHPQSWTQSNIKHMRKQL-DRLGLCFSWDREITTCLPDYYKWTQYLFIKLYEAGLAYQKEALVNWDPVDQTVLANEQVDEHGCSWRSGAKVEQKYLRQWFIKTTAYAKAMQDALADLP--EWYGIKGMQAHWIGDCVGCHLDFTLKVHGQATG---EKLTAYTATPEAIYG--TSHVAISPSH-------------RLLHGHSSL--KEALRMALVPGKDCLTPVMAVNMLTQQEVPVVILAKADLEGSLDSKIGIPSTSSEDTILAQTLGL--------AYSEVIETLPDGTERLSSSAEFTGMTRQDAFLALTQKARGKRVGGDVTSDKLKDWLISRQRYWGTPIPIVHCPVCGPTPVPLEDLPVTLP-----NIASFTGKGGPPLAMASEWVNCSCPRCKG-AAKRETDTMDTFVDSAWYYFRYTDPHNPHSPFNTAVADYWMPVDLYIGGKEHAVMHLFYARFFSHFCH---DQKMVKHREPFHKLLAQGLIKGQTFRLPSGQYLQREEVDLTGSVPVHAKTKEKLEVTWEKMSKSKHNGVDPEEVVEQYGIDTIRLYILFAAPPEKDILWDVKTDALPGVLRWQQRLWTLTTRFIEARASGKSPQPQLLSNKEKAEARKLWEYKNSVISQVTTHFTEDFSLNSAISQLMGLSNALSQASQSVILHSPEFEDALCALMVMAAPLAPHVTSEIWAGLA 804
BLAST of Leucine--tRNA ligase vs. Ensembl Human
Match: LARS2 (leucyl-tRNA synthetase 2, mitochondrial [Source:HGNC Symbol;Acc:HGNC:17095]) HSP 1 Score: 432.18 bits (1110), Expect = 2.243e-137 Identity = 264/732 (36.07%), Postives = 397/732 (54.23%), Query Frame = 2
Query: 377 RVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDL-VDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIKHPLSGRLIP------------IIRNQNL------KFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTL----RIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQ-ENDIVLGIQNWERRVWNLIKEIVNSPVN--DPKVFLVNDFLLQDSEFIAKYQ-SLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFXXXXXXXXXXXXXXXWAQLA 2491
+ +++ +VI PMGWDAFGLPAENAA++R P+ WT NI M++Q+ +++ L W RE++TC P+YYKWTQ+LF +L+E G YQK +VNWDP+D+TVLA+E +D G SWRSGA VE+ L+QWFI+TTA ++++ D L + +W + Q WIGD G ++DF L V Q EKL+ +T P A + V I+ + L H SL K L L+P ++ Q + K + + S++G+P +D LA LG+ AY+ ++++ +E + + L R +GG S DWLISRQRYWGTPIPIVHCP CGP P LPV LP + + PL+ A W+ CP+C A RE+DTMDTFVDS+WY+ RY D N + +V MPVD+YIGG EHA+ HL+YARF SHF H D K+ EPF + L GL+ QT++ SG+++ +V PV+ +E L W+KMSKSK NG+ P+ VV++YG+D RL IL P+K+ W + D + G+ W++R+W L + + + P+ L+++ ++ + +Y+ S+++++ + + F+++ +S L L++ + S S + L ++ PL+P ++SE+WA LA
Sbjct: 70 QASKISEADVINPMGWDAFGLPAENAAVERNLHPQSWTQSNIKHMRKQL-DRLGLCFSWDREITTCLPDYYKWTQYLFIKLYEAGLAYQKEALVNWDPVDQTVLANEQVDEHGCSWRSGAKVEQKYLRQWFIKTTAYAKAMQDALADLP--EWYGIKGMQAHWIGDCVGCHLDFTLKVHGQATG---EKLTAYTATPEAIYG--TSHVAISPSH-------------RLLHGHSSL--KEALRMALVPGKDCLTPVMAVNMLTQQEVPVVILAKADLEGSLDSKIGIPSTSSEDTILAQTLGL--------AYSEVIETLPDGTERLSSSAEFTGMTRQDAFLALTQKARGKRVGGDVTSDKLKDWLISRQRYWGTPIPIVHCPVCGPTPVPLEDLPVTLP-----NIASFTGKGGPPLAMASEWVNCSCPRCKG-AAKRETDTMDTFVDSAWYYFRYTDPHNPHSPFNTAVADYWMPVDLYIGGKEHAVMHLFYARFFSHFCH---DQKMVKHREPFHKLLAQGLIKGQTFRLPSGQYLQREEVDLTGSVPVHAKTKEKLEVTWEKMSKSKHNGVDPEEVVEQYGIDTIRLYILFAAPPEKDILWDVKTDALPGVLRWQQRLWTLTTRFIEARASGKSPQPQLLSNKEKAEARKLWEYKNSVISQVTTHFTEDFSLNSAISQLMGLSNALSQASQSVILHSPEFEDALCALMVMAAPLAPHVTSEIWAGLA 761
BLAST of Leucine--tRNA ligase vs. Ensembl Human
Match: LARS2 (leucyl-tRNA synthetase 2, mitochondrial [Source:HGNC Symbol;Acc:HGNC:17095]) HSP 1 Score: 404.445 bits (1038), Expect = 4.790e-130 Identity = 231/567 (40.74%), Postives = 318/567 (56.08%), Query Frame = 2
Query: 200 YDFNVKKQLETFW----QEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDL-VDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIKHPLSGRLIP------------IIRNQNL------KFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTL----RIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIH 1819
Y +K +E +W +E+ SKI++ D ++ K Y+LSMFPYPSG+LHMGH+RVYT +D +AR ++ +VI PMGWDAFGLPAENAA++R P+ WT NI M++Q+ +++ L W RE++TC P+YYKWTQ+LF +L+E G YQK +VNWDP+D+TVLA+E +D G SWRSGA VE+ L+QWFI+TTA ++++ D L + +W + Q WIGD G ++DF L V Q EKL+ +T P A + V I+ + L H SL K L L+P ++ Q + K + + S++G+P +D LA LG+ AY+ ++++ +E + + L R +GG S DWLISRQRYWGTPIPIVHCP CGP P LPV LP + + PL+ A W+ CP+C A RE+DTMDTFVDS+WY+ RY D N + +V MPVD+YIGG EHA+ HL+YARF SHF H
Sbjct: 51 YTLQTRKDVEKWWHQRIKEQASKISEADK-SKPKFYVLSMFPYPSGKLHMGHVRVYTISDTIARFQKMRGMQVINPMGWDAFGLPAENAAVERNLHPQSWTQSNIKHMRKQL-DRLGLCFSWDREITTCLPDYYKWTQYLFIKLYEAGLAYQKEALVNWDPVDQTVLANEQVDEHGCSWRSGAKVEQKYLRQWFIKTTAYAKAMQDALADLP--EWYGIKGMQAHWIGDCVGCHLDFTLKVHGQATG---EKLTAYTATPEAIYG--TSHVAISPSH-------------RLLHGHSSL--KEALRMALVPGKDCLTPVMAVNMLTQQEVPVVILAKADLEGSLDSKIGIPSTSSEDTILAQTLGL--------AYSEVIETLPDGTERLSSSAEFTGMTRQDAFLALTQKARGKRVGGDVTSDKLKDWLISRQRYWGTPIPIVHCPVCGPTPVPLEDLPVTLP-----NIASFTGKGGPPLAMASEWVNCSCPRCKG-AAKRETDTMDTFVDSAWYYFRYTDPHNPHSPFNTAVADYWMPVDLYIGGKEHAVMHLFYARFFSHFCH 579
BLAST of Leucine--tRNA ligase vs. Ensembl Celegans
Match: lars-2 (Leucyl Amino-acyl tRNA Synthetase [Source:UniProtKB/TrEMBL;Acc:Q23511]) HSP 1 Score: 389.423 bits (999), Expect = 1.207e-121 Identity = 251/742 (33.83%), Postives = 384/742 (51.75%), Query Frame = 2
Query: 218 KQLETFWQEKMS---KITQNDSAN-RKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVA--FVEINNDN--IWWNYXXXXXXXXXXLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDF-KDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTY-QTKSGKFIPNHQVKYLDDK-PVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFL---VNDFLLQDSEFIAKYQSLVTKILFQYQKSFT-ISVVVSVLQKLTSLYMKFSNEKAKS 2398
K LE W + + + ++N +N K YILSMFPYPSG+LH+GHMRVYT +D AR RL +EVI P+GWD+FGLPAENAA +G +P +WT+ NI MK+Q+ + L DW +E+STC+P +++WTQW+FC+LFE G V + S VNWDPIDKTVLA E IDN G+SWRSGA EK L QW I T ++ L +GL + QW V + Q WIG D+ F L V + + D EI+N + K + + + + ++GR IP+I ++ Q V+S L + +F KD L K F+ ++ K+ +E + G GGY S+ TDW++SRQR WGTPIP++ V N+LPV+ + K + G ++DT+DTF DS+WY+LRYLD+KN L S + ++MPVD+Y+GG+EHA H+++ARF+++F+ DI + EPF+ +P G+V +T+ + ++GK++ + V+ DK + D+ + ++KMSKSK NG+ ++D G+DMTRL +L P W E D+ GI+ R+ + +IV++ N + L ++ + + F + + ++L + + I + L+K+ +Y+ S E ++
Sbjct: 31 KMLEKHWSKSLVDSFESSKNKPSNLNGKKYILSMFPYPSGRLHIGHMRVYTISDATARYYRLNGYEVIHPIGWDSFGLPAENAARDKGVDPREWTINNIESMKQQLLKTKIL-FDWEKEISTCEPEFFRWTQWIFCKLFEYGLVKRTSSEVNWDPIDKTVLAAEQIDNDGKSWRSGAKAEKKKLSQWMIETPKYAKRLQEGLRKM-SEQWGEVADIQANWIGKC-------DVYRFM--------LPVMKDGSNDEIIDLRIKDIFEISNAEFLVLKQSHSLADKDATQFPYKLPFQVLNGVTGRQIPVIVVED---TYQPDVTSFLNSRIGNFQKDKILVEK----FAIAEPKHKRKLTKNDIQEMASFG--------------------GYGGYETSRTLTDWVVSRQRGWGTPIPMIQTKDGKRVAVQLNKLPVL------------------GSDRGQEVDKERFGESG----VFDTDTLDTFFDSAWYYLRYLDNKNKSELISKGAM-KNMPVDVYVGGIEHAAVHMFFARFVAYFLK---DINVIQTAEPFTDLIPQGIVRGKTFIEKETGKYLGSEDVQQSGDKLTLKNDSSVEVDAVYEKMSKSKNNGVDLAAILDSEGVDMTRLRLLEAAAPRAPINWGETDLK-GIKKLLDRIAAINSQIVDARDNGKPIELKKEIDGSIKETYNFFVRNVGMCLEVLHLHNTALMRIQGFTNALKKVDPIYLAHSPEGQRA 701
BLAST of Leucine--tRNA ligase vs. Ensembl Celegans
Match: lars-2 (Leucyl Amino-acyl tRNA Synthetase [Source:UniProtKB/TrEMBL;Acc:Q23511]) HSP 1 Score: 145.206 bits (365), Expect = 7.534e-37 Identity = 83/263 (31.56%), Postives = 150/263 (57.03%), Query Frame = 2
Query: 1628 ESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTY-QTKSGKFIPNHQVKYLDDK-PVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFL---VNDFLLQDSEFIAKYQSLVTKILFQYQKSFT-ISVVVSVLQKLTSLYMKFSNEKAKS 2398
++DT+DTF DS+WY+LRYLD+KN L S + ++MPVD+Y+GG+EHA H+++ARF+++F+ DI + EPF+ +P G+V +T+ + ++GK++ + V+ DK + D+ + ++KMSKSK NG+ ++D G+DMTRL +L P W E D+ GI+ R+ + +IV++ N + L ++ + + F + + ++L + + I + L+K+ +Y+ S E ++
Sbjct: 40 DTDTLDTFFDSAWYYLRYLDNKNKSELISKGAM-KNMPVDVYVGGIEHAAVHMFFARFVAYFLK---DINVIQTAEPFTDLIPQGIVRGKTFIEKETGKYLGSEDVQQSGDKLTLKNDSSVEVDAVYEKMSKSKNNGVDLAAILDSEGVDMTRLRLLEAAAPRAPINWGETDLK-GIKKLLDRIAAINSQIVDARDNGKPIELKKEIDGSIKETYNFFVRNVGMCLEVLHLHNTALMRIQGFTNALKKVDPIYLAHSPEGQRA 297
BLAST of Leucine--tRNA ligase vs. Ensembl Celegans
Match: iars-2 (Isoleucyl Amino-acyl tRNA Synthetase [Source:UniProtKB/TrEMBL;Acc:E2JL04]) HSP 1 Score: 61.6178 bits (148), Expect = 2.136e-9 Identity = 43/168 (25.60%), Postives = 72/168 (42.86%), Query Frame = 2
Query: 275 ANRKKMYILSMF---PYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAE-NAALQRGYEPEKWTLKNISEMK----EQMKEQMT------LSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGA 736
A RK I + PY +G+ H GH D + + + + V+F GWD GLP E Q+G + L+ + + E + +QM ++ DW + T P+Y +F +L E+ VY+ + V W P T LA+ ++ + + A
Sbjct: 81 AARKDSEIFELLDGPPYANGEAHTGHAINKILKDFVVKSRIALGYRVLFRPGWDCHGLPIELKIGKQQGNAGSRTPLETRAAARIVADEAIGKQMNAFRRWGVTADWEKPYVTKSPSYVAAQLDIFAKLVEQKLVYRSFKPVYWSPSSNTALAESELEYNDKHQSTSA 248
BLAST of Leucine--tRNA ligase vs. Ensembl Celegans
Match: glp-4 (Valine--tRNA ligase [Source:UniProtKB/Swiss-Prot;Acc:Q9U1Q4]) HSP 1 Score: 61.6178 bits (148), Expect = 2.598e-9 Identity = 39/150 (26.00%), Postives = 65/150 (43.33%), Query Frame = 2
Query: 311 PYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQR---------------GYEPEKWTLKNISEMKEQMKEQ---MTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELID 706
P +G LH+GH T D + R NR+ +F G D G+ + +R + E W KN E + + +Q + S+DW R + T DP + F R+ E G +Y+ + +VNW ++ ++D +D
Sbjct: 127 PNVTGNLHVGHALATTVEDTITRFNRMHGKRTLFNPGCDHAGIATQVVVEKRLKRERGLTRHDLGRDRFNQEVWHWKN--EKGDVIYDQFRKLGASVDWDRAVFTMDPKMCRAVTEAFIRMHESGTIYRSNRLVNWSCALRSAISDIEVD 274
BLAST of Leucine--tRNA ligase vs. Ensembl Celegans
Match: glp-4 (Valine--tRNA ligase [Source:UniProtKB/Swiss-Prot;Acc:Q9U1Q4]) HSP 1 Score: 61.6178 bits (148), Expect = 2.598e-9 Identity = 39/150 (26.00%), Postives = 65/150 (43.33%), Query Frame = 2
Query: 311 PYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQR---------------GYEPEKWTLKNISEMKEQMKEQ---MTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELID 706
P +G LH+GH T D + R NR+ +F G D G+ + +R + E W KN E + + +Q + S+DW R + T DP + F R+ E G +Y+ + +VNW ++ ++D +D
Sbjct: 127 PNVTGNLHVGHALATTVEDTITRFNRMHGKRTLFNPGCDHAGIATQVVVEKRLKRERGLTRHDLGRDRFNQEVWHWKN--EKGDVIYDQFRKLGASVDWDRAVFTMDPKMCRAVTEAFIRMHESGTIYRSNRLVNWSCALRSAISDIEVD 274
BLAST of Leucine--tRNA ligase vs. Ensembl Fly
Match: LeuRS-m (gene:FBgn0027085 transcript:FBtr0073348) HSP 1 Score: 542.347 bits (1396), Expect = 6.258e-180 Identity = 303/730 (41.51%), Postives = 423/730 (57.95%), Query Frame = 2
Query: 194 PHYDFNVKKQLETFWQEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTK-SGKFIPNHQVKYLDDK---PVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLV---NDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTS 2362
P +VK ++E W+E++S N ++ K Y+LSMFPYPSG LHMGH+RVYT AD +AR R+ V PMGWD+FGLPAENAA QRG EP WT +NI++MKEQ+K ++ S DW+ ELSTC P YYKWTQ LF L G YQ +VNWDP+DKTVLADE +D G SWRSGA VEK L+QWFIRT+A ++ LLDGL+ WR +I Q+ WIG+ G F+L+ L V+T HP L D AF+ + +++ KL SL+ +P +G +P++ + + F + V L P +D L G+ +F G + A W + L ++ L+ + +GGYR S DWLISRQRYWGTPIPIVHC CG V P+ QLPV LPP +AF +CPKCG +A RE+DTMDTFVDSSWY+LRYLD++NS+ + ++ + MPVD+YIGG EHA+ HLYYARF++HF+H L EPFS+ L G+V+ ++++ K SG+++P +V+ ++ K V + +EP+ W+KMSKSK NG+ P + ++YG D TRLIILA+ P +R W + GI NW++R+W +++ + + +V +FL +D++ V F Y+ + +SV +S +Q LT+
Sbjct: 32 PELTSDVKHRIEAHWREQLSGGQFNPKDSQDKYYVLSMFPYPSGNLHMGHVRVYTIADSVARFQRMCGKNVFQPMGWDSFGLPAENAANQRGVEPASWTEQNIAQMKEQLK-RLGCSFDWNHELSTCSPKYYKWTQHLFLMLHRHGLAYQNEALVNWDPVDKTVLADEQVDANGCSWRSGAKVEKKLLRQWFIRTSAYAKQLLDGLEDPTLRDWRDIINLQRHWIGECDGYA--FNLL-----TSASGLLRVWTTHP-EHLKDPHAFLVLRSNH-----------HLSKLKGADSLSAANPFAGTTMPVVFSDEVTFPPKSDVY--LAAPSFRGEDKDLWDSYGL--AFTSNGKDEESLSPA-----NWEM-------LRKEVLQAATRLNVGGYRVSSKLKDWLISRQRYWGTPIPIVHCQKCGAVPVPEEQLPVSLPP-------------KDSPKEAF---LCDCPKCGEKDARRETDTMDTFVDSSWYYLRYLDAQNSERIFDTALTKKFMPVDLYIGGKEHAVLHLYYARFINHFLH---SCGLSPTSEPFSRLLVQGMVMGRSFRVKGSGRYVPETEVEIVNAKKNQAVLKETKEPVVMTWEKMSKSKLNGVEPSDMFNEYGTDTTRLIILADVAPTSHRNW-SSATFPGILNWQKRLWLTLQDFQQAREDQTASDVVPTSEEFLAEDAKLFDARNFYVKGATFNYRHAQQLSVAISKMQGLTN 705
BLAST of Leucine--tRNA ligase vs. Ensembl Fly
Match: ValRS (gene:FBgn0027079 transcript:FBtr0087735) HSP 1 Score: 58.151 bits (139), Expect = 3.659e-8 Identity = 43/176 (24.43%), Postives = 77/176 (43.75%), Query Frame = 2
Query: 269 DSANRKKMYILSMFPYP--SGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMK-----------------EQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNT---GRSWRS 730
D+ N +++ + P P +G LH+GH D + R +R+ ++ G D G+ + + + EK + ++ K EQ+K + S DW+R T DP + F RL E+G +Y+ S +VNW ++ ++D +D GR++ S
Sbjct: 123 DAPNPNGKFVM-IIPPPNVTGSLHLGHALTNAIEDAITRYHRMKGRTTLWVPGCDHAGIATQVVVEKLLWRDEKLSRHDLGREKFIERIWDWRREKGGRIYEQLK-SLGSSYDWTRVAFTMDPKLCRAVTEAFVRLHEEGSIYRSSRLVNWSCTLRSAISDIEVDKVEIPGRTFLS 296
BLAST of Leucine--tRNA ligase vs. Ensembl Fly
Match: ValRS (gene:FBgn0027079 transcript:FBtr0087734) HSP 1 Score: 58.151 bits (139), Expect = 3.659e-8 Identity = 43/176 (24.43%), Postives = 77/176 (43.75%), Query Frame = 2
Query: 269 DSANRKKMYILSMFPYP--SGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMK-----------------EQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNT---GRSWRS 730
D+ N +++ + P P +G LH+GH D + R +R+ ++ G D G+ + + + EK + ++ K EQ+K + S DW+R T DP + F RL E+G +Y+ S +VNW ++ ++D +D GR++ S
Sbjct: 123 DAPNPNGKFVM-IIPPPNVTGSLHLGHALTNAIEDAITRYHRMKGRTTLWVPGCDHAGIATQVVVEKLLWRDEKLSRHDLGREKFIERIWDWRREKGGRIYEQLK-SLGSSYDWTRVAFTMDPKLCRAVTEAFVRLHEEGSIYRSSRLVNWSCTLRSAISDIEVDKVEIPGRTFLS 296
BLAST of Leucine--tRNA ligase vs. Ensembl Fly
Match: ValRS (gene:FBgn0027079 transcript:FBtr0333148) HSP 1 Score: 58.151 bits (139), Expect = 3.759e-8 Identity = 43/176 (24.43%), Postives = 77/176 (43.75%), Query Frame = 2
Query: 269 DSANRKKMYILSMFPYP--SGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMK-----------------EQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNT---GRSWRS 730
D+ N +++ + P P +G LH+GH D + R +R+ ++ G D G+ + + + EK + ++ K EQ+K + S DW+R T DP + F RL E+G +Y+ S +VNW ++ ++D +D GR++ S
Sbjct: 123 DAPNPNGKFVM-IIPPPNVTGSLHLGHALTNAIEDAITRYHRMKGRTTLWVPGCDHAGIATQVVVEKLLWRDEKLSRHDLGREKFIERIWDWRREKGGRIYEQLK-SLGSSYDWTRVAFTMDPKLCRAVTEAFVRLHEEGSIYRSSRLVNWSCTLRSAISDIEVDKVEIPGRTFLS 296
BLAST of Leucine--tRNA ligase vs. Ensembl Fly
Match: IleRS-m (gene:FBgn0036569 transcript:FBtr0302328) HSP 1 Score: 56.6102 bits (135), Expect = 1.108e-7 Identity = 41/138 (29.71%), Postives = 60/138 (43.48%), Query Frame = 2
Query: 311 PYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAE-----NAALQRGYEPEKWTLKNISEMKEQMKEQMT---LSIDWSRE--LSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLAD 694
PY +GQLHMGH D+ R V + GWD GLP E A Q E + + E + KE+ + + +W ++ T P + +F L+EKG VY+ + V W P +T LA+
Sbjct: 84 PYANGQLHMGHAVNKILKDITLRQRVAHGQRVNYIPGWDCHGLPIELKATSTAPGQSAQEIRQKSRAFAWEAIKSQKEEFSSWGILANWQKDNIYMTFQPEFIVNQLEMFYNLYEKGLVYRDLKPVYWSPSSRTALAE 221
BLAST of Leucine--tRNA ligase vs. Ensembl Zebrafish
Match: lars2 (leucyl-tRNA synthetase 2, mitochondrial [Source:ZFIN;Acc:ZDB-GENE-070928-3]) HSP 1 Score: 498.819 bits (1283), Expect = 1.980e-163 Identity = 295/791 (37.29%), Postives = 444/791 (56.13%), Query Frame = 2
Query: 200 YDFNVKKQLETFWQEKMS---KITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDL-VDFQDENRILEKLSVFTEHPVAALSDFVAFVEI-NNDNIWWNYXXXXXXXXXXLSFHR----SLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFV---RLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTK-SGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQ-ENDIVLGIQNWERRVWNLIKEI--VNSPVNDPKVFLVNDFLLQDSEFIAKYQS-LVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFXXXXXXXXXXXXXXXWAQLASKLAPIESIF 2521
Y ++++E +W ++ + D ++RKK Y+LSMFPYPSG+LHMGH+RVY +D ++ ++ H+V+ PMGWDAFGLPAENAA++R +PE WT NI M+ Q+ + + L +W RE++TC P+YYKWTQ+LF ++FE G YQK +VNWDP+D+TVLADE +D G SWRSGA VE+ LKQWFI+TT ++ LLD L + +W V Q WIGD +G + + L VD +D + LS ++ P A+V I + + KS L R + + L+GR +P++ + +F + + +G+P ++A +A LG+ +S V + ++ S E G + + N + +K R M +GG+ S DWLISRQRYWGTPIPIVHC SCGPV P+++LPV LP K + SPL A +W+ +CP+C P A RE+DTMDTFVDSSWY+ RY D +N S MPVD+YIGG EHA+ HLYYARF+SHF D + EPF + L GL+ QT+ + +G+++ + + PV + L+ W+KMSKSK NG+ P+ V+ +YG+ RL +L P+++ W + D + G+ W+ R+W L+ ++ V P ++N +++ I ++ +T++ + + F + +S L LT+ + S S+ + L I P+ P L+SELWA L+ +P+ S+
Sbjct: 38 YKAETRRRVEQWWHGRIQEQWRTDAEDQSSRKKFYVLSMFPYPSGRLHMGHVRVYAISDTISHFQKMRGHQVLNPMGWDAFGLPAENAAIERNLDPEDWTRSNIESMRTQL-DSLGLCFNWDREITTCLPDYYKWTQYLFIKMFEAGLAYQKEALVNWDPVDQTVLADEQVDENGCSWRSGAPVEQKMLKQWFIKTTNYAKPLLDALADLP--EWYGVKSMQANWIGDCSGCFFNHTLKVDGRDTGEV---LSAYSSSPETVFG--AAYVSILPSHRLLHGSSALKSALLRALRPGRDSLTEVTALNLLTGRELPVVISAKSQFSGH--LDTVIGIPDSSEEEAAVAQSLGLSWSSVLKTEADGTHTLINS--DEFTGQSREEAF-NSVTQKA----RQMNVGGHLTSAKLRDWLISRQRYWGTPIPIVHCGSCGPVAVPEDELPVTLP-----KIPALTGKGASPLKTAEDWLNCQCPRCKGP-AERETDTMDTFVDSSWYYFRYTDPQNPLRPFERSRADHWMPVDVYIGGKEHAVMHLYYARFLSHFCR---DQSMTAHREPFRKLLVQGLIKGQTFTLEHTGQYLKKEDIDFTGPDPVEKSSGRRLTVTWEKMSKSKHNGVDPQEVLQQYGISTLRLYLLYAAPPEQDILWDVKTDAIPGVLRWQSRLWGLVSKLRSVREGPETPNPSILNKRERSEAKKIWDNKNYTLTQVTGHFTEDFLFNAAISRLMGLTNTLSQASTRVVLHSVEFEDALASLCIMAAPMCPHLASELWAGLSQVRSPLGSVL 802
BLAST of Leucine--tRNA ligase vs. Ensembl Zebrafish
Match: lars2 (leucyl-tRNA synthetase 2, mitochondrial [Source:ZFIN;Acc:ZDB-GENE-070928-3]) HSP 1 Score: 233.417 bits (594), Expect = 3.806e-70 Identity = 103/198 (52.02%), Postives = 143/198 (72.22%), Query Frame = 2
Query: 197 HYDFNVKKQLETFWQEKMS---KITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTT 781
Y ++++E +W ++ + D ++RKK Y+LSMFPYPSG+LHMGH+RVY +D ++ ++ H+V+ PMGWDAFGLPAENAA++R +PE WT NI M+ Q+ + + L +W RE++TC P+YYKWTQ+LF ++FE G YQK +VNWDP+D+TVLADE +D G SWRSGA VE+ LKQWFI+TT
Sbjct: 48 EYKAETRRRVEQWWHGRIQEQWRTDAEDQSSRKKFYVLSMFPYPSGRLHMGHVRVYAISDTISHFQKMRGHQVLNPMGWDAFGLPAENAAIERNLDPEDWTRSNIESMRTQL-DSLGLCFNWDREITTCLPDYYKWTQYLFIKMFEAGLAYQKEALVNWDPVDQTVLADEQVDENGCSWRSGAPVEQKMLKQWFIKTT 244
BLAST of Leucine--tRNA ligase vs. Ensembl Zebrafish
Match: vars2 (valyl-tRNA synthetase 2, mitochondrial [Source:ZFIN;Acc:ZDB-GENE-060503-575]) HSP 1 Score: 59.3066 bits (142), Expect = 2.116e-8 Identity = 38/151 (25.17%), Postives = 69/151 (45.70%), Query Frame = 2
Query: 311 PYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEK------------------WTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDN 709
P +G LH+GH D LAR R+ ++V++ G D G+ A + ++R E+ W + E+ Q++ ++ S+DWSR T DP + F RL + G +Y+ ++NW ++ ++D +D+
Sbjct: 133 PNVTGTLHLGHALTVAIEDALARWRRMQGYKVLWVPGCDHAGI-ATQSVVERKLLREQGKSRQDFSREEFLKQVWMWKNEKGDEIYHQLR-KLGASLDWSRACFTMDPAFSSAVTEAFVRLCDSGLIYRAEGLINWSCTLESAISDIEVDS 281
BLAST of Leucine--tRNA ligase vs. Ensembl Zebrafish
Match: vars2 (valyl-tRNA synthetase 2, mitochondrial [Source:ZFIN;Acc:ZDB-GENE-060503-575]) HSP 1 Score: 59.3066 bits (142), Expect = 2.116e-8 Identity = 38/151 (25.17%), Postives = 69/151 (45.70%), Query Frame = 2
Query: 311 PYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEK------------------WTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDN 709
P +G LH+GH D LAR R+ ++V++ G D G+ A + ++R E+ W + E+ Q++ ++ S+DWSR T DP + F RL + G +Y+ ++NW ++ ++D +D+
Sbjct: 133 PNVTGTLHLGHALTVAIEDALARWRRMQGYKVLWVPGCDHAGI-ATQSVVERKLLREQGKSRQDFSREEFLKQVWMWKNEKGDEIYHQLR-KLGASLDWSRACFTMDPAFSSAVTEAFVRLCDSGLIYRAEGLINWSCTLESAISDIEVDS 281
BLAST of Leucine--tRNA ligase vs. Ensembl Zebrafish
Match: iars2 (isoleucyl-tRNA synthetase 2, mitochondrial [Source:ZFIN;Acc:ZDB-GENE-030616-608]) HSP 1 Score: 57.3806 bits (137), Expect = 8.872e-8 Identity = 62/227 (27.31%), Postives = 92/227 (40.53%), Query Frame = 2
Query: 188 IMPHYDFNVKKQLETFWQEKMSKITQNDSANR----------KKMYIL-SMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAAL-----------QRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLAD-ELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESL 799
++P DF +K + ++++ KI Q ++ KK Y L PY +G H+GH D+ R L +V + GWD GLP E AL Q + ++ K IS + + ++ DW T D Y +F + KG +YQ + V W P +T LA+ EL N RS I PL + TA +E L
Sbjct: 36 LLPRTDFPIKVNGPSLLEQEI-KIQQKCGFDQLYKWQREKKAKKEYCLHDGPPYANGDPHVGHALNKILKDIRNRFEVLRGRQVHYVPGWDCHGLPIELKALGDLGTNELTPVQIRQKAREFAEKAISRQRAAFQRWGVMA-DWDNCYYTFDGKYEAAQLKVFQEMHNKGLIYQDYKPVFWSPSSRTALAEAELEYNPEHVSRSLYIT--FPLLTLPAKLTAKAEGL 258
BLAST of Leucine--tRNA ligase vs. Ensembl Xenopus
Match: bmp8 (bone morphogenetic protein 8 (osteogenic protein 2) [Source:Xenbase;Acc:XB-GENE-485424]) HSP 1 Score: 479.945 bits (1234), Expect = 3.008e-155 Identity = 273/750 (36.40%), Postives = 434/750 (57.87%), Query Frame = 2
Query: 197 HYDFNVKKQLETFWQ----EKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAAL-SDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIK----HPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLE--NGLNEK-----CLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKS-GKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQ-ENDIVLGIQNWERRVWNLIKEIVNSPVND--PKVFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNE 2386
Y +K++E +W+ E+ +KI++ D + + K Y+LSMFPYPSG+LHMGH+RVYT +D +AR ++ +VI PMGWDAFGLPAENAA++ G P+ WT +NI M+EQ+ +++ L +W RE++TC +YYKWTQ+LF +++E G YQK +VNWDP+D+TVLA+E +D G SWRSGA VE+ LKQWF++TT ++ + D L + +W V Q WIG+ G + FD + D E +S ++ P A + +VA + N + K + + + + + + + +P++ + +F+ + +++G+P +DA LA KLG+ Y ++++++ ++G + N GL+ + + G+GG+ S DWLISRQRYWGTPIP++HCPSCG V P LPV+LP ++ + +SPL+ A W+ +CP+C A+RE+DTMDTFVDS+WY+LRY D NS+ + MPVD+YIGG EHA+ HLYYARF +HF + + EPF + L GL+ QTY+ S G+++ +V +P Y++ E L W+KMSKSK NG+ + +V++YG+D TRL +L P+++ W + D + G++ W+ R+W + + + + + P L+N + ++ I + ++L + +K IS++ S L +S + S+E
Sbjct: 48 EYGLETRKKVENWWKPKVKERFTKISKVDKS-KPKFYVLSMFPYPSGKLHMGHVRVYTISDAIARFQKMRGMQVINPMGWDAFGLPAENAAIEHGLRPDTWTERNIQHMREQL-DRIGLCFNWDREITTCSGDYYKWTQYLFLKMYEAGLAYQKEAMVNWDPVDQTVLANEQVDENGCSWRSGAKVEQKYLKQWFLKTTVYAKDMSDALADLP--EWYGVKSMQANWIGECNGCF--FDCMLKIDGVETGETISAYSFKPEAVFGASYVAI--LPNHRLLHGASSLKEALQKAYTKGKDCVTRVSALNLFTKQEVPVVISAKTEFEGY--LDTKIGIPSATPEDAELADKLGL--------LYVKVIETS---NDGTEMICNSGKFTGLSRQEAFLAITQQASSEGVGGHLTSDKLRDWLISRQRYWGTPIPVIHCPSCGTVPVPYEDLPVLLP-----SAASFTGKGLSPLASASEWINCKCPRCTGA-ASRETDTMDTFVDSAWYYLRYTDPLNSERPFDKAWADFWMPVDLYIGGKEHAVMHLYYARFFNHFC---SSQGMTKHREPFHKLLVQGLIKGQTYKLASTGRYLKKSEVDDTGFEPAYLETGEKLLVTWEKMSKSKHNGVDAQEIVEQYGIDTTRLYLLFAAPPEQDILWDVKTDAIPGVRRWQTRLWTITSKFIEARKSSSVPNPHLLNKKEVAEARRIWESRNLAVLEVVISRKCLNISLIHSYLNLTSSRVILHSSE 767
BLAST of Leucine--tRNA ligase vs. Ensembl Xenopus
Match: bmp8 (bone morphogenetic protein 8 (osteogenic protein 2) [Source:Xenbase;Acc:XB-GENE-485424]) HSP 1 Score: 475.707 bits (1223), Expect = 1.373e-153 Identity = 268/751 (35.69%), Postives = 434/751 (57.79%), Query Frame = 2
Query: 197 HYDFNVKKQLETFWQ----EKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAAL-SDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIK----HPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLE--NGLNEK-----CLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKS-GKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQ-ENDIVLGIQNWERRVWNLIKEIVNSPVND--PKVFLVNDFLLQDSEFI--AKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSN 2383
Y +K++E +W+ E+ +KI++ D + + K Y+LSMFPYPSG+LHMGH+RVYT +D +AR ++ +VI PMGWDAFGLPAENAA++ G P+ WT +NI M+EQ+ +++ L +W RE++TC +YYKWTQ+LF +++E G YQK +VNWDP+D+TVLA+E +D G SWRSGA VE+ LKQWF++TT ++ + D L + +W V Q WIG+ G + FD + D E +S ++ P A + +VA + N + K + + + + + + + +P++ + +F+ + +++G+P +DA LA KLG+ Y ++++++ ++G + N GL+ + + G+GG+ S DWLISRQRYWGTPIP++HCPSCG V P LPV+LP ++ + +SPL+ A W+ +CP+C A+RE+DTMDTFVDS+WY+LRY D NS+ + MPVD+YIGG EHA+ HLYYARF +HF + + EPF + L GL+ QTY+ S G+++ +V +P Y++ E L W+KMSKSK NG+ + +V++YG+D TRL +L P+++ W + D + G++ W+ R+W + + + + + P L+N + ++ I ++ +++ + + + + + +S L L++ + S+
Sbjct: 49 EYGLETRKKVENWWKPKVKERFTKISKVDKS-KPKFYVLSMFPYPSGKLHMGHVRVYTISDAIARFQKMRGMQVINPMGWDAFGLPAENAAIEHGLRPDTWTERNIQHMREQL-DRIGLCFNWDREITTCSGDYYKWTQYLFLKMYEAGLAYQKEAMVNWDPVDQTVLANEQVDENGCSWRSGAKVEQKYLKQWFLKTTVYAKDMSDALADLP--EWYGVKSMQANWIGECNGCF--FDCMLKIDGVETGETISAYSFKPEAVFGASYVAI--LPNHRLLHGASSLKEALQKAYTKGKDCVTRVSALNLFTKQEVPVVISAKTEFEGY--LDTKIGIPSATPEDAELADKLGL--------LYVKVIETS---NDGTEMICNSGKFTGLSRQEAFLAITQQASSEGVGGHLTSDKLRDWLISRQRYWGTPIPVIHCPSCGTVPVPYEDLPVLLP-----SAASFTGKGLSPLASASEWINCKCPRCTGA-ASRETDTMDTFVDSAWYYLRYTDPLNSERPFDKAWADFWMPVDLYIGGKEHAVMHLYYARFFNHFC---SSQGMTKHREPFHKLLVQGLIKGQTYKLASTGRYLKKSEVDDTGFEPAYLETGEKLLVTWEKMSKSKHNGVDAQEIVEQYGIDTTRLYLLFAAPPEQDILWDVKTDAIPGVRRWQTRLWTITSKFIEARKSSSVPNPHLLNKKEVAEARRIWESRNLAVLEVVTSHFTEDYLFNAAISQLMSLSNTLSQASS 769
BLAST of Leucine--tRNA ligase vs. Ensembl Xenopus
Match: bmp8 (bone morphogenetic protein 8 (osteogenic protein 2) [Source:Xenbase;Acc:XB-GENE-485424]) HSP 1 Score: 472.241 bits (1214), Expect = 3.779e-152 Identity = 270/743 (36.34%), Postives = 431/743 (58.01%), Query Frame = 2
Query: 197 HYDFNVKKQLETFWQ----EKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAAL-SDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIK----HPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLE--NGLNEK-----CLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKS-GKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQ-ENDIVLGIQNWERRVWNLIKEIVNSPVND--PKVFLVNDFLLQDSEFIAKYQSL-VTKILFQYQKSFTISVVVSVLQKLTS 2362
Y +K++E +W+ E+ +KIT ++S ++ K Y+LSMFPYPSG+LHMGH+RVYT +D +AR ++ +VI PMGWDAFGLPAENAA++ G P+ WT +NI M+EQ+ +++ L +W RE++TC +YYKWTQ+LF +++E G YQK +VNWDP+D+TVLA+E +D G SWRSGA VE+ LKQWF++TT ++ + D L + +W V Q WIG+ G + FD + D E +S ++ P A + +VA + N + K + + + + + + + +P++ + +F+ + +++G+P +DA LA KLG+ Y ++++++ ++G + N GL+ + + G+GG+ S DWLISRQRYWGTPIP++HCPSCG V P LPV+LP ++ + +SPL+ A W+ +CP+C A+RE+DTMDTFVDS+WY+LRY D NS+ + MPVD+YIGG EHA+ HLYYARF +HF + + EPF + L GL+ QTY+ S G+++ +V DD + E L W+KMSKSK NG+ + +V++YG+D TRL +L P+++ W + D + G++ W+ R+W + + + + + P L+N + ++ I + ++L V ++ + + + + +S L L++
Sbjct: 64 EYGLETRKKVENWWKPKVKERFTKITLDESKSKPKFYVLSMFPYPSGKLHMGHVRVYTISDAIARFQKMRGMQVINPMGWDAFGLPAENAAIEHGLRPDTWTERNIQHMREQL-DRIGLCFNWDREITTCSGDYYKWTQYLFLKMYEAGLAYQKEAMVNWDPVDQTVLANEQVDENGCSWRSGAKVEQKYLKQWFLKTTVYAKDMSDALADLP--EWYGVKSMQANWIGECNGCF--FDCMLKIDGVETGETISAYSFKPEAVFGASYVAI--LPNHRLLHGASSLKEALQKAYTKGKDCVTRVSALNLFTKQEVPVVISAKTEFEGY--LDTKIGIPSATPEDAELADKLGL--------LYVKVIETS---NDGTEMICNSGKFTGLSRQEAFLAITQQASSEGVGGHLTSDKLRDWLISRQRYWGTPIPVIHCPSCGTVPVPYEDLPVLLP-----SAASFTGKGLSPLASASEWINCKCPRCTGA-ASRETDTMDTFVDSAWYYLRYTDPLNSERPFDKAWADFWMPVDLYIGGKEHAVMHLYYARFFNHFC---SSQGMTKHREPFHKLLVQGLIKGQTYKLASTGRYLKKSEV---DDTGKHYG--EKLLVTWEKMSKSKHNGVDAQEIVEQYGIDTTRLYLLFAAPPEQDILWDVKTDAIPGVRRWQTRLWTITSKFIEARKSSSVPNPHLLNKKEVAEARRIWESRNLAVLEVTSHFTEDYLFNAAISQLMSLSN 772
BLAST of Leucine--tRNA ligase vs. Ensembl Xenopus
Match: bmp8 (bone morphogenetic protein 8 (osteogenic protein 2) [Source:Xenbase;Acc:XB-GENE-485424]) HSP 1 Score: 462.225 bits (1188), Expect = 2.793e-149 Identity = 274/740 (37.03%), Postives = 407/740 (55.00%), Query Frame = 2
Query: 200 YDFNVKKQLETFWQEKM-----SKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIM---GIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKS-GKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQ-ENDIVLGIQNWERRVWNLIKEIVNSPVND--PKVFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSN 2383
Y +K++E +W I Q S + K Y+LSMFPYPSG+LHMGH+RVYT +D +AR ++ +VI PMGWDAFGLPAENAA++ G P+ WT +NI M+EQ+ +++ L +W RE++TC +YYKWTQ+LF +++E G YQK +VNWDP+D+TVLA+E +D G SWRSGA VE+ LKQWF++TT ++ + D L + +W V Q WIG+ G + FD + D E +S ++ P A ++V I L HR L L L Q + +DCV+ L L +K +P +V SA E EG+ LD + E L + G+GG+ S DWLISRQRYWGTPIP++HCPSCG V P LPV+LP ++ + +SPL+ A W+ +CP+C A+RE+DTMDTFVDS+WY+LRY D NS+ + MPVD+YIGG EHA+ HLYYARF +HF + + EPF + L GL+ QTY+ S G+++ +V +P Y++ E L W+KMSKSK NG+ + +V++YG+D TRL +L P+++ W + D + G++ W+ R+W + + + + + P L+N + ++ I + ++L + + + + + +S L L++ + S+
Sbjct: 22 YGLETRKKVENWWNVAFLLNNWRTIGQYKS--KPKFYVLSMFPYPSGKLHMGHVRVYTISDAIARFQKMRGMQVINPMGWDAFGLPAENAAIEHGLRPDTWTERNIQHMREQL-DRIGLCFNWDREITTCSGDYYKWTQYLFLKMYEAGLAYQKEAMVNWDPVDQTVLANEQVDENGCSWRSGAKVEQKYLKQWFLKTTVYAKDMSDALADLP--EWYGVKSMQANWIGECNGCF--FDCMLKIDGVETGETISAYSFKPEAVFG--ASYVAI-------------------LPNHRLLHGASSLKEAL------QKAYTKGKDCVTRVSAL--------NLFTKQEVP-----------VVISAKTEFEGY-LDTKIGLSRQEAFLAITQQASSEGVGGHLTSDKLRDWLISRQRYWGTPIPVIHCPSCGTVPVPYEDLPVLLP-----SAASFTGKGLSPLASASEWINCKCPRCTGA-ASRETDTMDTFVDSAWYYLRYTDPLNSERPFDKAWADFWMPVDLYIGGKEHAVMHLYYARFFNHFC---SSQGMTKHREPFHKLLVQGLIKGQTYKLASTGRYLKKSEVDDTGFEPAYLETGEKLLVTWEKMSKSKHNGVDAQEIVEQYGIDTTRLYLLFAAPPEQDILWDVKTDAIPGVRRWQTRLWTITSKFIEARKSSSVPNPHLLNKKEVAEARRIWESRNLA--VTSHFTEDYLFNAAISQLMSLSNTLSQASS 696
BLAST of Leucine--tRNA ligase vs. Ensembl Xenopus
Match: bmp8 (bone morphogenetic protein 8 (osteogenic protein 2) [Source:Xenbase;Acc:XB-GENE-485424]) HSP 1 Score: 411.764 bits (1057), Expect = 1.181e-129 Identity = 239/690 (34.64%), Postives = 389/690 (56.38%), Query Frame = 2
Query: 368 VLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAAL-SDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIK----HPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLE--NGLNEK-----CLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKS-GKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQ-ENDIVLGIQNWERRVWNLIKEIVNSPVND--PKVFLVNDFLLQDSEFI--AKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSN 2383
V R ++ +VI PMGWDAFGLPAENAA++ G P+ WT +NI M+EQ+ +++ L +W RE++TC +YYKWTQ+LF +++E G YQK +VNWDP+D+TVLA+E +D G SWRSGA VE+ LKQWF++TT ++ + D L + +W V Q WIG+ G + FD + D E +S ++ P A + +VA + N + K + + + + + + + +P++ + +F+ + +++G+P +DA LA KLG+ Y ++++++ ++G + N GL+ + + G+GG+ S DWLISRQRYWGTPIP++HCPSCG V P LPV+LP ++ + +SPL+ A W+ +CP+C A+RE+DTMDTFVDS+WY+LRY D NS+ + MPVD+YIGG EHA+ HLYYARF +HF + + EPF + L GL+ QTY+ S G+++ +V +P Y++ E L W+KMSKSK NG+ + +V++YG+D TRL +L P+++ W + D + G++ W+ R+W + + + + + P L+N + ++ I ++ +++ + + + + + +S L L++ + S+
Sbjct: 66 VKERFTKISKVDVINPMGWDAFGLPAENAAIEHGLRPDTWTERNIQHMREQL-DRIGLCFNWDREITTCSGDYYKWTQYLFLKMYEAGLAYQKEAMVNWDPVDQTVLANEQVDENGCSWRSGAKVEQKYLKQWFLKTTVYAKDMSDALADLP--EWYGVKSMQANWIGECNGCF--FDCMLKIDGVETGETISAYSFKPEAVFGASYVAI--LPNHRLLHGASSLKEALQKAYTKGKDCVTRVSALNLFTKQEVPVVISAKTEFEGY--LDTKIGIPSATPEDAELADKLGL--------LYVKVIETS---NDGTEMICNSGKFTGLSRQEAFLAITQQASSEGVGGHLTSDKLRDWLISRQRYWGTPIPVIHCPSCGTVPVPYEDLPVLLP-----SAASFTGKGLSPLASASEWINCKCPRCTGA-ASRETDTMDTFVDSAWYYLRYTDPLNSERPFDKAWADFWMPVDLYIGGKEHAVMHLYYARFFNHFC---SSQGMTKHREPFHKLLVQGLIKGQTYKLASTGRYLKKSEVDDTGFEPAYLETGEKLLVTWEKMSKSKHNGVDAQEIVEQYGIDTTRLYLLFAAPPEQDILWDVKTDAIPGVRRWQTRLWTITSKFIEARKSSSVPNPHLLNKKEVAEARRIWESRNLAVLEVVTSHFTEDYLFNAAISQLMSLSNTLSQASS 726
BLAST of Leucine--tRNA ligase vs. Ensembl Mouse
Match: Lars2 (leucyl-tRNA synthetase, mitochondrial [Source:MGI Symbol;Acc:MGI:2142973]) HSP 1 Score: 511.146 bits (1315), Expect = 2.260e-167 Identity = 286/752 (38.03%), Postives = 427/752 (56.78%), Query Frame = 2
Query: 200 YDFNVKKQLETFW----QEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDL-VDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIKHPLSGRLIPIIRNQNLKFQS-----------QDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTL----RIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQ-ENDIVLGIQNWERRVWNLIKEIVNSPVND--PKVFLVNDFLLQDSEFIAKYQ-SLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSN 2383
Y +K +E +W +E+ S++++ D + K Y+LSMFPYPSG+LHMGH+RVYT +D +AR ++ +VI PMGWDAFGLPAENAA++R PE WT NI M++Q+ +++ L W RE++TC P+YYKWTQ+LF +L+E G YQK +VNWDP+D+TVLA+E ++ G SWRSGA VEK L+QWFI+TTA ++++ D L + +W + Q WIGD G ++DF L VD +D EKL+ +T P A I++ I ++ S K + ++L P L P++ L Q + + S++G+P +D LA LG+P Y+ +++++ +E + + L R M +GG+ S DWLISRQRYWGTPIPIVHCP+CGPV P LPV+LP + SPL+ A W+ CP+C A RE+DTMDTFVDS+WY+ RY D N+ ++ MPVD+YIGG EHA+ HL+YARF+SHF H D K+ EPF + L GL+ QT++ SG+ + + + PV +E L W+KMSKSK NG+ P+ +V +YG+D RL IL P+K+ W + D + G+ W++R+W+L + + + P+ L+N ++ + +Y+ +++ ++ + + F ++ VVS L L+S + S
Sbjct: 50 YTLQTRKDVEKWWHQQIKEQASRVSEEDKL-KPKFYLLSMFPYPSGKLHMGHVRVYTLSDTIARFQKMRGMQVINPMGWDAFGLPAENAAIERNLHPESWTQSNIKHMRKQL-DRLGLCFSWDREITTCLPDYYKWTQYLFIKLYEAGLAYQKEALVNWDPVDQTVLANEQVNEYGCSWRSGAKVEKKYLRQWFIKTTAYAKAMQDALADLP--EWYGIKGMQAHWIGDCVGCHLDFTLKVDGEDTG---EKLTAYTATPEA-------IYGISHVAISPSHGLLHGCSSVKKALQKALV---PGRDCLTPVMAVSMLTLQEVPIVIMANPDLEGSLDSKIGIPSTSSEDTRLAQALGLP--------YSEVIEASPDGTERLSGSAEFTGMTRQDAFVALTRKARGMRVGGHVTSNKLKDWLISRQRYWGTPIPIVHCPACGPVPVPLQDLPVILP-----SIASLTGRGGSPLATALEWVNCSCPRCKG-SAKRETDTMDTFVDSAWYYFRYTDPHNTQSPFGSALADFWMPVDLYIGGKEHAVMHLFYARFLSHFCH---DQKMVKHREPFHKLLAQGLIKGQTFRLPSGQCLKKEDIDFTGPAPVCAKTKEKLEVTWEKMSKSKHNGVDPEEIVAQYGIDTIRLYILFAAPPEKDILWDVKTDALPGVLRWQQRLWSLTTRFIEARTSGTVPQPQLLNSKEKTKAQNLWEYKNAVIAQVTTHFTEDFALNSVVSQLMGLSSALSQASQ 767
BLAST of Leucine--tRNA ligase vs. Ensembl Mouse
Match: Lars2 (leucyl-tRNA synthetase, mitochondrial [Source:MGI Symbol;Acc:MGI:2142973]) HSP 1 Score: 272.322 bits (695), Expect = 2.111e-81 Identity = 148/364 (40.66%), Postives = 217/364 (59.62%), Query Frame = 2
Query: 200 YDFNVKKQLETFW----QEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDL-VDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIKHPLSGRLIPIIRNQNLKFQS-----------QDCVSSRLGLPLVDFKDATLASKLGIPFSFV 1243
Y +K +E +W +E+ S++++ D + K Y+LSMFPYPSG+LHMGH+RVYT +D +AR ++ +VI PMGWDAFGLPAENAA++R PE WT NI M++Q+ +++ L W RE++TC P+YYKWTQ+LF +L+E G YQK +VNWDP+D+TVLA+E ++ G SWRSGA VEK L+QWFI+TTA ++++ D L + +W + Q WIGD G ++DF L VD +D EKL+ +T P A I++ I ++ S K + ++L P L P++ L Q + + S++G+P +D LA LG+P+S V
Sbjct: 50 YTLQTRKDVEKWWHQQIKEQASRVSEEDKL-KPKFYLLSMFPYPSGKLHMGHVRVYTLSDTIARFQKMRGMQVINPMGWDAFGLPAENAAIERNLHPESWTQSNIKHMRKQL-DRLGLCFSWDREITTCLPDYYKWTQYLFIKLYEAGLAYQKEALVNWDPVDQTVLANEQVNEYGCSWRSGAKVEKKYLRQWFIKTTAYAKAMQDALADLP--EWYGIKGMQAHWIGDCVGCHLDFTLKVDGEDTG---EKLTAYTATP-------EAIYGISHVAISPSHGLLHGCSSVKKALQKALV---PGRDCLTPVMAVSMLTLQEVPIVIMANPDLEGSLDSKIGIPSTSSEDTRLAQALGLPYSEV 396
BLAST of Leucine--tRNA ligase vs. Ensembl Mouse
Match: Lars2 (leucyl-tRNA synthetase, mitochondrial [Source:MGI Symbol;Acc:MGI:2142973]) HSP 1 Score: 105.531 bits (262), Expect = 2.406e-26 Identity = 46/83 (55.42%), Postives = 61/83 (73.49%), Query Frame = 2
Query: 311 PYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSR 559
PYPSG+LHMGH+RVYT +D +AR ++ +VI PMGWDAFGLPAENAA++R PE WT NI M++Q+ +++ L W R
Sbjct: 2 PYPSGKLHMGHVRVYTLSDTIARFQKMRGMQVINPMGWDAFGLPAENAAIERNLHPESWTQSNIKHMRKQL-DRLGLCFSWDR 83
BLAST of Leucine--tRNA ligase vs. Ensembl Mouse
Match: Iars2 (isoleucine-tRNA synthetase 2, mitochondrial [Source:MGI Symbol;Acc:MGI:1919586]) HSP 1 Score: 63.929 bits (154), Expect = 5.967e-10 Identity = 54/183 (29.51%), Postives = 80/183 (43.72%), Query Frame = 2
Query: 311 PYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQR-GYEPEKWTLKNISE-----MKEQMKEQMTLSI------DWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLAD-ELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTV 820
PY +G H+GH D+ R + + +V F GWD GLP E L G + + + I E + +++Q + + DW+ T DP Y +F +++EKG VY+ + V W P +T LA+ EL N RS I + PL +R ESL D V
Sbjct: 116 PYANGDPHVGHALNKILKDIANRFHMMRGSKVHFVPGWDCHGLPIETKVLSELGVDAQSLSAMEIREKARSFAQAAIEKQKSAFVRWGVMADWNNCYYTFDPKYEAKQLRVFYQMYEKGLVYRSYKPVYWSPSSRTALAEAELEYNPEHVSRS--IYVRFPL----LRPPPKLESLTDASSPV 292
BLAST of Leucine--tRNA ligase vs. Ensembl Mouse
Match: Iars2 (isoleucine-tRNA synthetase 2, mitochondrial [Source:MGI Symbol;Acc:MGI:1919586]) HSP 1 Score: 63.5438 bits (153), Expect = 9.021e-10 Identity = 54/183 (29.51%), Postives = 80/183 (43.72%), Query Frame = 2
Query: 311 PYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQR-GYEPEKWTLKNISE-----MKEQMKEQMTLSI------DWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLAD-ELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTV 820
PY +G H+GH D+ R + + +V F GWD GLP E L G + + + I E + +++Q + + DW+ T DP Y +F +++EKG VY+ + V W P +T LA+ EL N RS I + PL +R ESL D V
Sbjct: 116 PYANGDPHVGHALNKILKDIANRFHMMRGSKVHFVPGWDCHGLPIETKVLSELGVDAQSLSAMEIREKARSFAQAAIEKQKSAFVRWGVMADWNNCYYTFDPKYEAKQLRVFYQMYEKGLVYRSYKPVYWSPSSRTALAEAELEYNPEHVSRS--IYVRFPL----LRPPPKLESLTDASSPV 292
BLAST of Leucine--tRNA ligase vs. UniProt/SwissProt
Match: sp|Q8VDC0|SYLM_MOUSE (Probable leucine--tRNA ligase, mitochondrial OS=Mus musculus OX=10090 GN=Lars2 PE=1 SV=1) HSP 1 Score: 511.146 bits (1315), Expect = 1.581e-166 Identity = 286/752 (38.03%), Postives = 427/752 (56.78%), Query Frame = 2
Query: 200 YDFNVKKQLETFW----QEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDL-VDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIKHPLSGRLIPIIRNQNLKFQS-----------QDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTL----RIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQ-ENDIVLGIQNWERRVWNLIKEIVNSPVND--PKVFLVNDFLLQDSEFIAKYQ-SLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSN 2383
Y +K +E +W +E+ S++++ D + K Y+LSMFPYPSG+LHMGH+RVYT +D +AR ++ +VI PMGWDAFGLPAENAA++R PE WT NI M++Q+ +++ L W RE++TC P+YYKWTQ+LF +L+E G YQK +VNWDP+D+TVLA+E ++ G SWRSGA VEK L+QWFI+TTA ++++ D L + +W + Q WIGD G ++DF L VD +D EKL+ +T P A I++ I ++ S K + ++L P L P++ L Q + + S++G+P +D LA LG+P Y+ +++++ +E + + L R M +GG+ S DWLISRQRYWGTPIPIVHCP+CGPV P LPV+LP + SPL+ A W+ CP+C A RE+DTMDTFVDS+WY+ RY D N+ ++ MPVD+YIGG EHA+ HL+YARF+SHF H D K+ EPF + L GL+ QT++ SG+ + + + PV +E L W+KMSKSK NG+ P+ +V +YG+D RL IL P+K+ W + D + G+ W++R+W+L + + + P+ L+N ++ + +Y+ +++ ++ + + F ++ VVS L L+S + S
Sbjct: 50 YTLQTRKDVEKWWHQQIKEQASRVSEEDKL-KPKFYLLSMFPYPSGKLHMGHVRVYTLSDTIARFQKMRGMQVINPMGWDAFGLPAENAAIERNLHPESWTQSNIKHMRKQL-DRLGLCFSWDREITTCLPDYYKWTQYLFIKLYEAGLAYQKEALVNWDPVDQTVLANEQVNEYGCSWRSGAKVEKKYLRQWFIKTTAYAKAMQDALADLP--EWYGIKGMQAHWIGDCVGCHLDFTLKVDGEDTG---EKLTAYTATPEA-------IYGISHVAISPSHGLLHGCSSVKKALQKALV---PGRDCLTPVMAVSMLTLQEVPIVIMANPDLEGSLDSKIGIPSTSSEDTRLAQALGLP--------YSEVIEASPDGTERLSGSAEFTGMTRQDAFVALTRKARGMRVGGHVTSNKLKDWLISRQRYWGTPIPIVHCPACGPVPVPLQDLPVILP-----SIASLTGRGGSPLATALEWVNCSCPRCKG-SAKRETDTMDTFVDSAWYYFRYTDPHNTQSPFGSALADFWMPVDLYIGGKEHAVMHLFYARFLSHFCH---DQKMVKHREPFHKLLAQGLIKGQTFRLPSGQCLKKEDIDFTGPAPVCAKTKEKLEVTWEKMSKSKHNGVDPEEIVAQYGIDTIRLYILFAAPPEKDILWDVKTDALPGVLRWQQRLWSLTTRFIEARTSGTVPQPQLLNSKEKTKAQNLWEYKNAVIAQVTTHFTEDFALNSVVSQLMGLSSALSQASQ 767
BLAST of Leucine--tRNA ligase vs. UniProt/SwissProt
Match: sp|Q5RDP4|SYLM_PONAB (Probable leucine--tRNA ligase, mitochondrial OS=Pongo abelii OX=9601 GN=LARS2 PE=2 SV=1) HSP 1 Score: 499.204 bits (1284), Expect = 8.202e-162 Identity = 297/795 (37.36%), Postives = 441/795 (55.47%), Query Frame = 2
Query: 200 YDFNVKKQLETFW----QEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDL-VDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIKHPLSGRLIP------------IIRNQNL------KFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTL----RIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQ-ENDIVLGIQNWERRVWNLIKEIVNSPVN--DPKVFLVNDFLLQDSEFIAKYQ-SLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFXXXXXXXXXXXXXXXWAQLA 2491
Y +K +E +W +E+ SKI++ D ++ K Y+LSMFPYPSG+LHMGH+RVYT +D +AR ++ +VI PMGWDAFGLPAENAA++R PE WT NI M++Q+ +++ L W RE++TC P+YYKWTQ+LF +L+E G YQK +VNWDP+D+TVLA+E +D G SWRSGA VE+ L+QWFI+TTA ++++ D L + +W + Q WIGD G ++DF L V Q EKL+ +T P A + V I+ + L H SL K L L+P ++ Q + K + + S++G+P +D LA LG+ AY+ ++++ +E + + L R +GG S DWLISRQRYWGTPIPIVHCP CGP P LPV LP + + SPL+ A W+ CP+C A RE+DTMDTFVDS+WY+ RY D N + +V MPVD+YIGG EHA+ HL+YARF SHF H D K+ EPF + L GL+ QT++ SG+++ +V PV+ +E L W+KMSKSK NG+ P+ VV++YG+D RL IL P+K+ W + D + G+ W++R+W L + + + P+ L+++ ++ + +Y+ S+++++ + + F+++ +S L L+ + S S + L ++ P++P ++SE+WA LA
Sbjct: 51 YTLQTRKDVEKWWHQRIKEQASKISEADK-SKPKFYVLSMFPYPSGKLHMGHVRVYTISDTIARFQKMRGMQVINPMGWDAFGLPAENAAVERNLHPESWTQSNIKHMRKQL-DRLGLCFSWDREITTCLPDYYKWTQYLFIKLYEAGLAYQKEALVNWDPVDQTVLANEQVDEHGCSWRSGAKVEQKYLRQWFIKTTAYAKAMQDALADLP--EWYGIKGMQAHWIGDCVGCHLDFTLKVHGQATG---EKLTAYTATPEAIYG--TSHVAISPSH-------------RLLHGHSSL--KEALRMALVPGKDCLTPVMAVNMLTQQEVPVVILAKADLEGSLDSKIGIPSTSSEDTILAQTLGL--------AYSEVIETLPDGTERLSSSAEFTGMTRQDAFLALTQKARGKRVGGDVTSDKLKDWLISRQRYWGTPIPIVHCPVCGPTPVPLEDLPVTLP-----NIASFTGKGGSPLAMASEWVNCSCPRCKG-AAKRETDTMDTFVDSAWYYFRYTDPHNPHSPFNTAVADYWMPVDLYIGGKEHAVMHLFYARFFSHFCH---DQKMVKHREPFHKLLAQGLIKGQTFRLPSGQYLQREEVDLTGSVPVHAKTKEKLEVTWEKMSKSKHNGVDPEEVVEQYGIDTIRLYILFAAPPEKDILWDVKTDALPGVLRWQQRLWTLTTRFIEARASGKSPQPQLLSNKEKAEARKLWEYKNSVISQVTTHFTEDFSLNSAISQLMGLSGALSQASQSVILHSPEFEDALCALMVMAAPMAPHVTSEIWAGLA 804
BLAST of Leucine--tRNA ligase vs. UniProt/SwissProt
Match: sp|Q15031|SYLM_HUMAN (Probable leucine--tRNA ligase, mitochondrial OS=Homo sapiens OX=9606 GN=LARS2 PE=1 SV=2) HSP 1 Score: 497.278 bits (1279), Expect = 4.190e-161 Identity = 296/795 (37.23%), Postives = 441/795 (55.47%), Query Frame = 2
Query: 200 YDFNVKKQLETFW----QEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDL-VDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIKHPLSGRLIP------------IIRNQNL------KFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTL----RIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQ-ENDIVLGIQNWERRVWNLIKEIVNSPVN--DPKVFLVNDFLLQDSEFIAKYQ-SLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFXXXXXXXXXXXXXXXWAQLA 2491
Y +K +E +W +E+ SKI++ D ++ K Y+LSMFPYPSG+LHMGH+RVYT +D +AR ++ +VI PMGWDAFGLPAENAA++R P+ WT NI M++Q+ +++ L W RE++TC P+YYKWTQ+LF +L+E G YQK +VNWDP+D+TVLA+E +D G SWRSGA VE+ L+QWFI+TTA ++++ D L + +W + Q WIGD G ++DF L V Q EKL+ +T P A + V I+ + L H SL K L L+P ++ Q + K + + S++G+P +D LA LG+ AY+ ++++ +E + + L R +GG S DWLISRQRYWGTPIPIVHCP CGP P LPV LP + + PL+ A W+ CP+C A RE+DTMDTFVDS+WY+ RY D N + +V MPVD+YIGG EHA+ HL+YARF SHF H D K+ EPF + L GL+ QT++ SG+++ +V PV+ +E L W+KMSKSK NG+ P+ VV++YG+D RL IL P+K+ W + D + G+ W++R+W L + + + P+ L+++ ++ + +Y+ S+++++ + + F+++ +S L L++ + S S + L ++ PL+P ++SE+WA LA
Sbjct: 51 YTLQTRKDVEKWWHQRIKEQASKISEADK-SKPKFYVLSMFPYPSGKLHMGHVRVYTISDTIARFQKMRGMQVINPMGWDAFGLPAENAAVERNLHPQSWTQSNIKHMRKQL-DRLGLCFSWDREITTCLPDYYKWTQYLFIKLYEAGLAYQKEALVNWDPVDQTVLANEQVDEHGCSWRSGAKVEQKYLRQWFIKTTAYAKAMQDALADLP--EWYGIKGMQAHWIGDCVGCHLDFTLKVHGQATG---EKLTAYTATPEAIYG--TSHVAISPSH-------------RLLHGHSSL--KEALRMALVPGKDCLTPVMAVNMLTQQEVPVVILAKADLEGSLDSKIGIPSTSSEDTILAQTLGL--------AYSEVIETLPDGTERLSSSAEFTGMTRQDAFLALTQKARGKRVGGDVTSDKLKDWLISRQRYWGTPIPIVHCPVCGPTPVPLEDLPVTLP-----NIASFTGKGGPPLAMASEWVNCSCPRCKG-AAKRETDTMDTFVDSAWYYFRYTDPHNPHSPFNTAVADYWMPVDLYIGGKEHAVMHLFYARFFSHFCH---DQKMVKHREPFHKLLAQGLIKGQTFRLPSGQYLQREEVDLTGSVPVHAKTKEKLEVTWEKMSKSKHNGVDPEEVVEQYGIDTIRLYILFAAPPEKDILWDVKTDALPGVLRWQQRLWTLTTRFIEARASGKSPQPQLLSNKEKAEARKLWEYKNSVISQVTTHFTEDFSLNSAISQLMGLSNALSQASQSVILHSPEFEDALCALMVMAAPLAPHVTSEIWAGLA 804
BLAST of Leucine--tRNA ligase vs. UniProt/SwissProt
Match: sp|B7KCI7|SYL_GLOC7 (Leucine--tRNA ligase OS=Gloeothece citriformis (strain PCC 7424) OX=65393 GN=leuS PE=3 SV=1) HSP 1 Score: 483.026 bits (1242), Expect = 3.326e-156 Identity = 281/697 (40.32%), Postives = 399/697 (57.25%), Query Frame = 2
Query: 212 VKKQLETFWQEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQW-RRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFT----------------EHPVA----------ALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNR----IVKSAWKESEGWNLDHNLENGLNEKCLKTLRI-----MGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQT-KSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKE 2191
++K+ + W E T D+ + K Y LSMFPYPSG LHMGH+R Y DV+AR+ R+ H V+ PMGWDAFGLPAENAA++RG P KWT +NI++M++Q+ +Q+ LSIDWSRE++TC P+YY+WTQWLF + F+ G YQK VNWDP+D+TVLA+E +D+ G+SWRSGA VE+ L+QWF++ T +E LL+ LD + G W +V Q WIG G Y++F +V EK++VFT EHP+ A+ F+ V ++ + L+ +++ +P +G+ +PI+ + ++ + +G+P D +D A + +P V + + + A+ E G ++ +G++ KT I G G R DWLISRQRYWG PIP++HCPSCG V PD LPV LP + E SPL+K +W+ CP CG P A RE+DTMDTF+DSSWYFLRY D+ + S V++ M VD Y+GG+EHAI HL Y+RF + + D L + EPF + L G+V TY+ K+GK+IP+ QV D P + EPLS F++KMSKSK+NG+ PK V+ KYG D R+ IL P+K+ +W + D V G + +VW ++ E
Sbjct: 10 IEKKWQQTWVEMGLDQTPEDN-TKPKYYALSMFPYPSGNLHMGHVRNYVITDVIARLKRMQGHRVLHPMGWDAFGLPAENAAIERGIHPAKWTYQNIAQMRQQL-QQLGLSIDWSREVTTCSPDYYQWTQWLFLQFFQAGLAYQKEAAVNWDPVDQTVLANEQVDSEGKSWRSGAKVERKMLRQWFLKITDYAEQLLNDLDKLPG--WPEKVKLMQANWIGKSIGAYLEFPIVGMD------EKIAVFTTRPDTVYGVTYVVLAPEHPLTPKVTTAKNKKAVEKFIKEVTSESEQ---ERTAEDKPKKGILTGGKAI---NPFNGQEVPILIANYVLYEY--GTGAVMGVPAHDIRDFKFAQENKLPIRVVIVPEDQQDQDVTLTQAYTEP-GIMVNSGSFDGMDSITGKTAIIDYAEKQGFGKARVQYRLRDWLISRQRYWGCPIPVIHCPSCGTVPVPDEDLPVKLP-----EDVEFSGRGASPLAKLDSWINVPCPSCGEP-AKRETDTMDTFIDSSWYFLRYTDANDQQTAFSLDKVNDWMSVDQYVGGVEHAILHLLYSRFFTKVVR---DRGLVDVDEPFQRLLTQGMVQGMTYKNPKTGKYIPSSQVNPED--PKDPETGEPLSVFYEKMSKSKYNGVDPKQVLAKYGADTARMFILFKAPPEKDLEWDDAD-VEGQFRFLNKVWRIVGE 675
BLAST of Leucine--tRNA ligase vs. UniProt/SwissProt
Match: sp|B7JYA9|SYL_RIPO1 (Leucine--tRNA ligase OS=Rippkaea orientalis (strain PCC 8801) OX=41431 GN=leuS PE=3 SV=1) HSP 1 Score: 479.174 bits (1232), Expect = 8.688e-155 Identity = 294/753 (39.04%), Postives = 418/753 (55.51%), Query Frame = 2
Query: 224 LETFWQE---KMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQW-RRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFT----------------EHPV----------AALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKES---EGWNLDHNLENGLNEKCLKTLRI-----MGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQT-KSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPK-VFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTS 2362
+E WQ+ K +++N+ K Y LSMFPYPSG LHMGH+R Y DV+AR+ RL + V+ PMGWDAFGLPAENAA+ RG P +WT KN+S+MK+Q+ + + LSIDW RE++TC P YYKWTQWLF +L++ G YQK VNWDPID+TVLA+E +D+ G SWRSGA VE+ L+QWF++ T +E LL LD + G W RV Q+ WIG G Y++F + + EK++VFT EHP+ AA+ F+ E++N++ + ++ AI +P +G+ IPI+ + ++ + +G+P D +D A + +P V + + E+ G ++ + +G+ KT I G G R DWLISRQRYWG PIP+VHCPSCG V PD LPV LP ++ E SPL+K +W+ CP CG P A RE+DTMDTF+DSSWY+LRY D+ N+ + ++ M +D Y+GG+EHAI HL Y+RF F D L N EPF + L G+V TY+ ++GK++P+ V + DD + E LS F++KMSKSK+NG+ PK V+ KYG D R+ IL P+K+ +W + D V G + RVW L + N N PK V ++ +D + + + +I + + + VS L KL++
Sbjct: 10 IEQKWQQDWVKQGLDKTPENSNKPKFYALSMFPYPSGNLHMGHVRNYVITDVIARLKRLQGYRVLHPMGWDAFGLPAENAAIDRGIPPAEWTYKNMSQMKQQL-QTLGLSIDWEREVATCAPEYYKWTQWLFLQLYQAGLAYQKEAAVNWDPIDQTVLANEQVDSEGYSWRSGAKVERKLLRQWFLKITDYAEQLLTDLDKLPG--WPDRVKLMQENWIGKSVGAYLEFPIKGSE------EKIAVFTTRPDTVYGVTYVVLAPEHPLTLKVTTPEQKAAVDAFIE--EVSNES---EIERTAEDKPKRGILTGGTAI-NPFNGQEIPILIADYVLYEY--GTGAVMGVPAHDTRDFKFAKEKQLPIKVVIIPENSDNTNPPLTEAYTDPGIMVNSGIFDGIQSTEGKTAIIEYAEKEGYGKARIQYRLRDWLISRQRYWGCPIPVVHCPSCGTVAVPDADLPVKLP-----ENVEFTGRDASPLAKLEDWINVPCPSCGEP-AKRETDTMDTFIDSSWYYLRYTDANNNQEAFNLEKANDWMAIDQYVGGIEHAILHLLYSRF---FTKALRDRGLVNIDEPFKRLLTQGMVQGLTYKNPQTGKYVPSENVIFQDDHYRDKETGEILSFFYEKMSKSKYNGVDPKQVLGKYGADTARMFILFKAPPEKDLEWDDAD-VEGQFRFLNRVWRL---VANYLENKPKTVKKASELTKEDKDLRRAIHTAIKEISEDLEGDYQFNTAVSELMKLSN 732
BLAST of Leucine--tRNA ligase vs. TrEMBL
Match: A0A2T7NI18 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_18980 PE=3 SV=1) HSP 1 Score: 578.556 bits (1490), Expect = 0.000e+0 Identity = 337/771 (43.71%), Postives = 449/771 (58.24%), Query Frame = 2
Query: 215 KKQLETFW----QEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLN-----EKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTK-SGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLVNDFLLQDSEFIAKYQSL-VTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFXXXXXXXXXXXXXXXWAQLAS 2494
+K++E W +E+ + + +++K Y+LSMFPYPSG+LHMGH+RVYT +DV+AR RL VI PMGWDAFGLPAENAA++RG P++WT KNI MKEQ+ EQ+ S DW+RE +TCDP YY+WTQ +F ++FE G VYQK VNWDP+D TVLADE ID GRSWRSGAIVEK LKQW+I+TTA ++SL DGL V W+ +I Q+ WIG G + F L EN + E L ++T P A VA++ ++ D+ + S+A HP +G IPI+ +++ +F + V LG+P + LA L + Y I+ E H+L NGL E L G+GG SK DWLISRQRYWGTPIPI+HC +C V P +QLPV LPP S SPLS+ +W+ CP+CG EA RE DTMDTFVDSSWYFLRYLD N+ V ++MPVD+YIGG EHA HLY+ARF HF+H D+ L EPF L G+VL ++++ K +GK++ QV + KPV D + PL E W+KMSKSK NG+ P+ V+ ++G+D TRL IL+ P +RKW ND +GI W+ R+W L+ +NS + + L + E + + ++L V++ Q+ K F ++ +S +Q TS K A S Y R L D I L P+ P L ELW LAS
Sbjct: 63 RKEIEKHWIPLIKEEDRLQSLQEDCSKEKYYVLSMFPYPSGKLHMGHVRVYTISDVMARFQRLQGRYVIHPMGWDAFGLPAENAAIERGERPDEWTYKNIKNMKEQL-EQLGCSFDWNREFATCDPAYYRWTQHIFLKMFEAGLVYQKEGEVNWDPVDMTVLADEQIDEHGRSWRSGAIVEKQYLKQWYIKTTAKAQSLYDGLHEVNAELWKDIISLQQDWIGKCDGTQLKFALEG--QENMVFEPLHIYTPTPEAVYG--VAYIAVSPDH--------RLNQQHLYKKILSVAAVHPFTGERIPIVVSKSTQFDYHNNV--MLGIPSISEASRCLADSLNL--------KYLEILGKRADGDECIVNSHDL-NGLTRSEAFEAVLHIALEKGVGGEHVSKRGRDWLISRQRYWGTPIPIIHCNTCQAVPVPFDQLPVELPP-----ISGLTGHGPSPLSQQHDWVNVTCPRCGG-EAKREIDTMDTFVDSSWYFLRYLDPHNTSAPYKRDTVDKYMPVDLYIGGKEHATMHLYFARFFCHFLH---DVGLLGRREPFDSLLTQGMVLGKSFRVKETGKYLKADQVDFTGTKPVEKDTKNPLVEEWEKMSKSKHNGVDPEDVLKEFGVDATRLCILSAVSPQSDRKWS-NDAFVGILRWQVRIWKLVGSYINSFTSASHTKCSDAELQEWEENLFEARNLVVSRATIQFSKKFLLNTAISHMQDFTSTLGKVPGHVAMESQQYQRCLADLCIMLAPICPSLMCELWTALAS 799
BLAST of Leucine--tRNA ligase vs. TrEMBL
Match: A0A4Z2D8S1 (Putative leucine--tRNA ligase OS=Schistosoma japonicum OX=6182 GN=EWB00_003633 PE=4 SV=1) HSP 1 Score: 576.244 bits (1484), Expect = 0.000e+0 Identity = 314/747 (42.03%), Postives = 436/747 (58.37%), Query Frame = 2
Query: 404 VIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDL---VDFQDEN---------------------RILEKLSVFTEHPVAALSDFVAFVEINNDNIWW---------NYXXXXXXXXXXLSFHRSL--AIKHPLSGRLIPIIRNQ----------------NLKFQSQDCVSSRLGLPLVD---FKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTY-QTKSGKFIPNHQV--KYLDDKPVY---IDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVN--------DPKVFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSN---EKAKSSIFYHRMLLDF 2431
I PMGWD+FGLPAENAA+ R P WT KNI MK+Q+ E M+LS+DWSRELSTCDPNYYKWTQWLF +L++ Y + VNWDPIDKTVLADELIDN G SWRSGA VE+ PL+QWF RTT SESLLDGL ++ QWR V++ Q+GW+G L G ++F++ V+ D N + E+L+VFT++P A++D V+ + + D++++ N + ++ F + L +++HP +GR IP+IR +L ++ DC+S L +PL F S L ++ + E G + ++ K ++ LR+ GGY CS RTDWL+SRQRYWGTPIPI+HC SCG V P++QLPV+LPP + K + +W K+ CPKCG P A+RE+DT+DTFVDSSWY+LRYLD +N+ +C + +PVD+YIGG EHA+RHLYYARF++HF+H DI + EPF +FLP+GLVL QTY ++G+FI +V K+L K V ++ P+ +WDKMSKSKFNGI P VVDK+G+D RL +LAN GP + RKW E D++ GI NW ++ LI++++ + + K + + L+ F +QS++ ++ Y +F IS ++ LQ++T L K S SS Y R L D
Sbjct: 6 TICPMGWDSFGLPAENAAIDRNEHPLNWTRKNIMSMKQQINETMSLSVDWSRELSTCDPNYYKWTQWLFLKLYQSDLAYHRPAYVNWDPIDKTVLADELIDNQGCSWRSGAKVERRPLRQWFFRTTVYSESLLDGLKDIENNQWRDVVQLQRGWLGHLNGTQMEFNIYSSVEQHDCNPRHHHTIFQSSFKCEHIKNVIQENERLAVFTKYPNLAVADLVSHIRVGPDSVYFADIYRKGKLNNMRPQVWISGEIHFSQILDISVRHPFTGRSIPMIRENIRLSKLSRAYPNELVFSLNGRTSDCISELLDIPLPPGKLFSAEKDLSNLSKSDVISEDDLKRCVIFTPISELHGKTVSESM-----NKAMQILRVNKRGGYWCSDERTDWLVSRQRYWGTPIPIIHCDSCGAVPVPEDQLPVLLPPLENPLKRGDEPLKHNE-----SWRKTTCPKCGKP-ASRETDTLDTFVDSSWYYLRYLDPQNNSSMCDWKKAANSLPVDVYIGGAEHAVRHLYYARFIAHFLH---DISILPSREPFRRFLPVGLVLGQTYVDPETGRFISTQRVNKKHLSGKDVQYYEVETNTPVQMYWDKMSKSKFNGIDPSEVVDKHGMDTVRLTMLANVGPHRTRKWTEGDVLYGINNWLNKISRLIEQLIEYSTSLQNSNIPWELKCNMDDPLLVHTPNFFKYHQSIIKRVNGYYDDTFVISSAIARLQEMTELLRKSSTLVGGPGPSSFMYLRALADL 738
BLAST of Leucine--tRNA ligase vs. TrEMBL
Match: A0A158R813 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS4697 PE=3 SV=1) HSP 1 Score: 573.548 bits (1477), Expect = 0.000e+0 Identity = 316/803 (39.35%), Postives = 457/803 (56.91%), Query Frame = 2
Query: 245 KMSKITQNDS-ANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQ--DENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAI-----------------------------KHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYN----------RIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCG-------------------------PVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTK-SGKFIPNHQVKYLDDKPVYID-NQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLV---NDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFS--NEKAKSSIFYHRMLLDF 2431
K +++ + D K YILSMFPYPSG+LHMGH+RVYT DVL R +L+ ++VI PMGWDAFGLPAENAA+ RG P++WT KNI M++Q++ M LS DW+RE+STC+PNYY+WTQWLF +LFE G Y++ VNWDPIDKTVLA+EL+D GRSWRSGA+VEK SL DGL ++G QWR VI Q+GWIG G ++FDL DF +E+ E+L+VFT+HP A + ++F+ + +I+WN + ++ +++ R LA+ KH L+ IP+I + L+ + + + LG P V + L+ L +P ++ RI +S E G NL +++ R+ YRCS+ R DW +SRQRYW TPIP+++C +CG V P+ LPV LPP + + W + CP CG+ A RE DT+DTFVDSSWY+LR+LD NS +CS +H+PVDIY+GG+EHAIRHL+YARF++HF+H ++ L EPF +FLP+GLV+ +T+++ +G++ P +++ + E + E W+KMSKSK NG+ P V ++G+++TRL +LA+ GP R+W E +I+ G++NW+ R+W LI ++ N ++ D+LL D +F+ Y +V ++ Y +SF +S V++ LQKLTS+ +K S EK SS Y + L D
Sbjct: 3 KCAQLLRTDGFVESKVFYILSMFPYPSGRLHMGHLRVYTVTDVLVRYYQLLGNQVIHPMGWDAFGLPAENAAIDRGVLPDEWTCKNIQNMRKQLRNDMKLSFDWTREISTCNPNYYRWTQWLFIKLFEAGLAYRRLAEVNWDPIDKTVLANELVDAEGRSWRSGALVEK--------------RSLKDGLGEIRGQQWRDVIRMQEGWIGPNDGFVVEFDL-DFHSTEEDLQGERLAVFTKHPGLAAAGAISFIAVGPQSIFWNERFRFPQNLREVNGSRKLAVVSLCPSLGKLSVQPAASCYPWPERLNISVKHLLTDTYIPLIYDPELRSRVEYETAVELGTPDVCDRHRELSRFLDLPSPECKIDVNCPAETDIELRIRIKQSPLLEFNGLNLKEATALAVSKLTGSEYRL-----YRCSRYRKDWSVSRQRYWATPIPLIYCRNCGVICLKFETLSKLQRFTAFKALGPFQTVPVPEEDLPVELPPLKAPLKRGDVPLRENT-----EWRYTTCPSCGS-SAEREVDTLDTFVDSSWYYLRFLDPTNSKEICSRDNAHKHIPVDIYVGGVEHAIRHLFYARFIAHFLH--RELGLLPCREPFQRFLPVGLVMGRTFRSSTTGQYFPAQEIEKDSSGNARVKATGEVVVESWEKMSKSKLNGVDPSEVFARHGVELTRLTMLASVGPHAARQWNEGEILRGVKNWQSRLWKLIGRLIELSNNSSTLWPSADQADYLLADEDFLQTYAHIVEQVHHHYGESFVLSAVIANLQKLTSILLKHSQVGEKCMSSRTYLKALADL 777
BLAST of Leucine--tRNA ligase vs. TrEMBL
Match: A0A2J7RII5 (Putative leucine--tRNA ligase, mitochondrial OS=Cryptotermes secundus OX=105785 GN=B7P43_G05917 PE=3 SV=1) HSP 1 Score: 566.229 bits (1458), Expect = 0.000e+0 Identity = 318/750 (42.40%), Postives = 441/750 (58.80%), Query Frame = 2
Query: 209 NVKKQLETFWQEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAI--KHPLSGRLIPIIRNQNLKF-QSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSC-GPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTK-SGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLVNDFLLQDSEFIAKYQSL-------VTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRML 2422
++K +E W+ K+ +D + K Y+LSMFPYPSGQLHMGH+RVYT +D +A R+ +VI PMGWDAFGLPAENAA++RG EP WT +NI MKEQ+ + + S +W REL+TCDPNYY WTQ+LF +L+E+G VY+K +VNWDP+D+TVLADE +D SWRSGA VEK L QWFIRTT S+SLLDGLD WR +I Q+ WIG+ G +F+L D + + L++FT +P + +F+ + +I K D + E + L + K+P +G +PI +++ + DC LG+P + D A+ IP S + + E ++ N + +K +GIGGY CS DWLISRQRYWGTPIPIV+C C GP P ++LPV LP + + SPL +A +W+ ++CPKCG +ATRE+DTMDTFVDSSWYFLRYLD N D S S +E MPVD+YIGG EHA+ HLYYARFM+HF+H I L EPF + L G+V+ Q+Y+ K +G+++P V + KPV + W+KMSKSK NG+ P+TV+ YG+D TRL+ILA+ P NR W +D GI W+ R+W ++ +N+ N + V+D +L EF + + + F Y +F +SV +S +Q LT+ + +E + + R L
Sbjct: 36 SIKHDIERKWRNKLQASVHDDDNQKSKFYVLSMFPYPSGQLHMGHVRVYTISDTMAHFYRMNGKKVIHPMGWDAFGLPAENAAIERGIEPFAWTKQNIKHMKEQL-QNLGCSFEWDRELATCDPNYYHWTQFLFLKLYEEGLVYKKEALVNWDPVDQTVLADEQVDTNCCSWRSGAKVEKKLLNQWFIRTTCFSKSLLDGLDDPVLKNWRDIINLQRHWIGECNGTKFEFELEDTLSQ-VMKHTLTIFTCNPEYVCN--ASFIAVAPGSILDRLKFDCDSTVESNDVFKRLPVMAKNPFTGERLPIYVTDQVEYEEGMDC---HLGIPGISEMDRLFATAANIPISSPKENS------QQLNSVEDVDVARGRINHMAQK-------LGIGGYPCSSKLRDWLISRQRYWGTPIPIVYCKQCNGPQSVPYSELPVTLP-----EIERLSNWGKSPLLEATDWLNTKCPKCGG-DATRETDTMDTFVDSSWYFLRYLDPHNEDMPFSKSKAAEFMPVDLYIGGKEHAVLHLYYARFMNHFLH---SIGLVPYKEPFQRLLVQGMVMGQSYRIKGTGQYLPKENVDFSGPKPVEKGTGLDVVSSWEKMSKSKHNGVHPETVLKDYGIDTTRLLILADVAPTSNRHWT-SDTFPGILKWQHRLWLTMRSFLNTRSNLQNI--VSDTVLHTPEFKEHEARMFDSRNYYLKGVTFNYTTAFQLSVAISKMQGLTNSLRRVPSEVIGRGLQFERAL 753
BLAST of Leucine--tRNA ligase vs. TrEMBL
Match: A0A2J7RII9 (Uncharacterized protein OS=Cryptotermes secundus OX=105785 GN=B7P43_G05917 PE=3 SV=1) HSP 1 Score: 565.459 bits (1456), Expect = 0.000e+0 Identity = 318/750 (42.40%), Postives = 441/750 (58.80%), Query Frame = 2
Query: 209 NVKKQLETFWQEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAI--KHPLSGRLIPIIRNQNLKF-QSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSC-GPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTK-SGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLVNDFLLQDSEFIAKYQSL-------VTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRML 2422
++K +E W+ K+ +D + K Y+LSMFPYPSGQLHMGH+RVYT +D +A R+ +VI PMGWDAFGLPAENAA++RG EP WT +NI MKEQ+ + + S +W REL+TCDPNYY WTQ+LF +L+E+G VY+K +VNWDP+D+TVLADE +D SWRSGA VEK L QWFIRTT S+SLLDGLD WR +I Q+ WIG+ G +F+L D + + L++FT +P + +F+ + +I K D + E + L + K+P +G +PI +++ + DC LG+P + D A+ IP S + + E ++ N + +K +GIGGY CS DWLISRQRYWGTPIPIV+C C GP P ++LPV LP + + SPL +A +W+ ++CPKCG +ATRE+DTMDTFVDSSWYFLRYLD N D S S +E MPVD+YIGG EHA+ HLYYARFM+HF+H I L EPF + L G+V+ Q+Y+ K +G+++P V + KPV + W+KMSKSK NG+ P+TV+ YG+D TRL+ILA+ P NR W +D GI W+ R+W ++ +N+ N + V+D +L EF + + + F Y +F +SV +S +Q LT+ + +E + + R L
Sbjct: 36 SIKHDIERKWRNKLQASVHDDDNQKSKFYVLSMFPYPSGQLHMGHVRVYTISDTMAHFYRMNGKKVIHPMGWDAFGLPAENAAIERGIEPFAWTKQNIKHMKEQL-QNLGCSFEWDRELATCDPNYYHWTQFLFLKLYEEGLVYKKEALVNWDPVDQTVLADEQVDTNCCSWRSGAKVEKKLLNQWFIRTTCFSKSLLDGLDDPVLKNWRDIINLQRHWIGECNGTKFEFELEDTLSQ-VMKHTLTIFTCNPEYVCN--ASFIAVAPGSILDRLKFDCDSTVESNDVFKRLPVMAKNPFTGERLPIYVTDQVEYEEGMDC---HLGIPGISEMDRLFATAANIPISSPKENS------QQLNSVEDVDVARGRINHMAQK-------LGIGGYPCSSKLRDWLISRQRYWGTPIPIVYCKQCNGPQSVPYSELPVTLP-----EIERLSNWGKSPLLEATDWLNTKCPKCGG-DATRETDTMDTFVDSSWYFLRYLDPHNEDMPFSKSKAAEFMPVDLYIGGKEHAVLHLYYARFMNHFLH---SIGLVPYKEPFQRLLVQGMVMGQSYRIKGTGQYLPKENVDFSGPKPVEKGTGLDVVSSWEKMSKSKHNGVHPETVLKDYGIDTTRLLILADVAPTSNRHWT-SDTFPGILKWQHRLWLTMRSFLNTRSNLQNI--VSDTVLHTPEFKEHEARMFDSRNYYLKGVTFNYTTAFQLSVAISKMQGLTNSLRRVPSEVIGRGLQFERAL 753
BLAST of Leucine--tRNA ligase vs. Ensembl Cavefish
Match: lars2 (leucyl-tRNA synthetase 2, mitochondrial [Source:ZFIN;Acc:ZDB-GENE-070928-3]) HSP 1 Score: 416.772 bits (1070), Expect = 8.760e-132 Identity = 274/773 (35.45%), Postives = 411/773 (53.17%), Query Frame = 2
Query: 392 MNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRS----------LAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFV---RLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQ-TKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQ-ENDIVLGIQNWERRVWNLIKEI--VNSPVNDPKVFLVNDFLLQDSEFIAKYQS-LVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFXXXXXXXXXXXXXXXWAQLASKLAPIESIFIETDDSYKSIFGPWGYNLNLPVWDQRLNVNEITLDIQNESVNIVN 2656
+ +V+ PMGWDAFGLPAENAA++RG +PE WT + I + E++TC P+YYKWTQ+LF +L+E G YQK +VNWDPID+TVLADE +D G SWRSGA VE+ L+QWFI+TT ++ LLD L + +W V Q WIG+ +G + FD V + E L+ +T P A AF+ +I +++ S + + HR+ + + +G+ +P++ + +FQ + + +G+P +D +A +LG+ + V + I+ SA E G + N + +K R +GG+ S DWLISRQRYWGTPIPIVHC SCGPV P +LPV LP K S + SPL A W+ CP+C P A RE+DTMDTFVDS+WY+ RY D N S+ MPVD+YIGG EHA+ HLYYARF+SHF D + + EPF + L GL+ QT++ ++SG+++ + + +PV D+ + L W+KMSKSK NG+ P+ VV +YG+D RL IL P+++ W + D + G+ W+ R+W L+ ++ P L+N +++ I +Y++ + ++ + + F ++ +S L LT+ + S S+ + + L + P++P L+SELWA L+ P+ S D + PW P D T++I N++ V
Sbjct: 75 IKEQVLNPMGWDAFGLPAENAAIERGLDPEDWTKRYICHCYPFSLLFNLFNNKLPFEITTCLPDYYKWTQYLFVKLYEAGLAYQKEAMVNWDPIDQTVLADEQVDENGCSWRSGAPVEQKLLRQWFIKTTNYAKPLLDALADLP--EWYGVKSMQANWIGECSGCF--FDHVLKLEGKETGEVLAAYTSTPEAVYG--AAFI-----SILPSHRLLHGSSPVRAALHRAFQPGKNSLTEVTALNVFTGQELPVVISAKNEFQGH--LDTVIGIPDSSEEDVCVARELGVSWDTVLKSEEDGTHTIINSA--EFTGQAPEEAF-NSITQKA----RQQKVGGHLTSSKLRDWLISRQRYWGTPIPIVHCESCGPVAVPVEELPVELP-----KVSLLSGKGASPLRSAHEWVNCSCPRCKGP-AQRETDTMDTFVDSAWYYFRYTDPHNKLRPFQRSLADHWMPVDVYIGGKEHAVMHLYYARFLSHFCK---DQGMVSHREPFKKLLVQGLIKGQTFRVSESGQYLKKEDIDFTGPEPVQKDSGKQLQVTWEKMSKSKHNGLDPQEVVQQYGIDTVRLYILYAAPPEQDILWDVKTDALPGVLRWQSRLWGLVTKLREARQAGETPNPSLLNKKERLEAKKIWEYKNHTIMQVTTNFTEDFLLNAAISRLMGLTNTLSQASPRVVLHSVEFEQALASLCLMAAPMAPHLTSELWAGLSQVKNPLSSSLTADGDVRQQ---PW------PTVDPEFLQTPDTVEINNKACGSVT 809
BLAST of Leucine--tRNA ligase vs. Ensembl Cavefish
Match: vars2 (valyl-tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:103044052]) HSP 1 Score: 64.3142 bits (155), Expect = 5.080e-10 Identity = 47/185 (25.41%), Postives = 82/185 (44.32%), Query Frame = 2
Query: 233 FWQEKMSKITQNDSANRKKMYILSMFPYP--SGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPE-----------------KWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRS 730
F EK S++ Q + +R Y P P +G LH+GH D LAR R+ ++V++ G D G+ ++ +R E +W + E+ Q+K ++ S+DW R T DP + F R+ + G +Y+ +VNW ++ ++D +D+ S R+
Sbjct: 123 FSPEKHSELPQ--AVDR---YFSLCIPPPNVTGTLHLGHALTVAIEDALARWRRMQGYKVLWVPGCDHAGIATQSVVERRLLREEGKRRQDFSRDEFLQEVWRWKKEKGEEIYHQLK-KLGASLDWRRACFTMDPGFSSAVTEAFVRMCDSGLIYRSESLVNWSCALESAISDIEVDSKQLSGRT 301
BLAST of Leucine--tRNA ligase vs. Ensembl Cavefish
Match: vars2 (valyl-tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:103044052]) HSP 1 Score: 64.3142 bits (155), Expect = 5.515e-10 Identity = 47/185 (25.41%), Postives = 82/185 (44.32%), Query Frame = 2
Query: 233 FWQEKMSKITQNDSANRKKMYILSMFPYP--SGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPE-----------------KWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRS 730
F EK S++ Q + +R Y P P +G LH+GH D LAR R+ ++V++ G D G+ ++ +R E +W + E+ Q+K ++ S+DW R T DP + F R+ + G +Y+ +VNW ++ ++D +D+ S R+
Sbjct: 123 FSPEKHSELPQ--AVDR---YFSLCIPPPNVTGTLHLGHALTVAIEDALARWRRMQGYKVLWVPGCDHAGIATQSVVERRLLREEGKRRQDFSRDEFLQEVWRWKKEKGEEIYHQLK-KLGASLDWRRACFTMDPGFSSAVTEAFVRMCDSGLIYRSESLVNWSCALESAISDIEVDSKQLSGRT 301
BLAST of Leucine--tRNA ligase vs. Ensembl Cavefish
Match: vars1 (valyl-tRNA synthetase 1 [Source:ZFIN;Acc:ZDB-GENE-010601-1]) HSP 1 Score: 53.9138 bits (128), Expect = 8.690e-7 Identity = 38/149 (25.50%), Postives = 62/149 (41.61%), Query Frame = 2
Query: 311 PYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAA-----LQRGY------------EPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELID 706
P +G LH+GH D L R +R+ ++ G D G+ + +RG E KW + + Q+K ++ S+DW R T DP Q F R+ E+G +Y+ +VNW + ++D +D
Sbjct: 289 PNVTGSLHLGHALTNAIQDCLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLMRERGMSRHDLGRENFIQEVWKWKNEKGDRIYHQLK-KLGSSLDWDRACFTMDPKLSYAVQEAFIRMHEEGVIYRSKRLVNWSCTLNSAISDIEVD 436
BLAST of Leucine--tRNA ligase vs. Ensembl Cavefish
Match: iars2 (isoleucyl-tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:103029967]) HSP 1 Score: 52.7582 bits (125), Expect = 1.977e-6 Identity = 39/154 (25.32%), Postives = 62/154 (40.26%), Query Frame = 2
Query: 263 QNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMT----------LSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLAD 694
Q + +K+ + PY +G H+GH D+ R L +V + GWD GLP E AL + L+ + +E +E + + DW T + Y +F + KG +YQ + V W P +T LA+
Sbjct: 99 QRERKAKKEFCLHDGPPYANGDAHVGHALNKILKDIHNRFEMLRGRKVHYVPGWDCHGLPIELKALGELQAKDMTPLQIRQKAREFAEEAIARQRAAFQRWGVMADWENCYYTFNGQYEAAQLRVFEDMHSKGLIYQDYKPVFWSPSTRTALAE 252
BLAST of Leucine--tRNA ligase vs. Ensembl Sea Lamprey
Match: iars2 (isoleucyl-tRNA synthetase 2, mitochondrial [Source:ZFIN;Acc:ZDB-GENE-030616-608]) HSP 1 Score: 60.077 bits (144), Expect = 1.920e-9 Identity = 54/182 (29.67%), Postives = 81/182 (44.51%), Query Frame = 2
Query: 263 QNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTL------KNISEMKEQMKEQMTLSI-------DWSRELSTCDPNYYKWTQW-LFCRLFEKGFVYQKSEIVNWDPIDKTVLAD-ELIDNTGRSWRSGAIVEKVPLKQ 763
Q + ++K+ + PY +G H+GH+ D+ R + ++V F GWD GLP E AL +K TL K E E+ E+ S +W + + Y+ Q +F + EKGF+YQ + V W P +T LA+ EL N + S A+ K PL Q
Sbjct: 87 QKERKSKKEFCLHDGPPYANGDPHVGHVLNKVLKDITNRYYVMRGYKVHFVPGWDCHGLPIELKALTESEVNDK-TLSPLDIRKKAREFAEKAIEKQRASFIRWGIMANWKDDCYSTFSKEYEAEQLKVFYNMHEKGFIYQDYKPVFWSPSTRTALAEAELEYNPAHT--SKAVHVKFPLAQ 265
BLAST of Leucine--tRNA ligase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000584.1 (pep scaffold:Pmarinus_7.0:GL486116:2002:8298:-1 gene:ENSPMAG00000000527.1 transcript:ENSPMAT00000000584.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 50.8322 bits (120), Expect = 2.714e-7 Identity = 39/157 (24.84%), Postives = 63/157 (40.13%), Query Frame = 2
Query: 272 SANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENA--ALQRGYEPEKWTLKNISEMKEQMKE--------------QMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLAD 694
S +R + P+ +G H GH+ T DV+ R V GWD GLP E + PE I E Q + ++ ID+ + T P++ + W+F +LF+KGFVY +++ + T L++
Sbjct: 35 SKSRPQYTFYDGPPFATGLPHYGHILAGTIKDVVTRFAHQSGFHVDRRFGWDCHGLPVEYEIDKMLNIKGPEDVAKMGIVEYNNQCRNIVMRYAKEWETTVIRLGRWIDFKNDYKTLYPSFMETVWWVFRQLFDKGFVYSGVKVMPYSTACTTPLSN 191
BLAST of Leucine--tRNA ligase vs. Ensembl Yeast
Match: NAM2 (Mitochondrial leucyl-tRNA synthetase; also has direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance; human homolog LARS2 can complement yeast null mutant, and is implicated in Perrault syndrome [Source:SGD;Acc:S000004374]) HSP 1 Score: 429.483 bits (1103), Expect = 4.338e-137 Identity = 281/768 (36.59%), Postives = 405/768 (52.73%), Query Frame = 2
Query: 236 WQEKMSK-ITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQW-RRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDN-IWWNYXXXXXXXXXXLSFHRSLA-----------IK--HPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKD-------------ATLASKLGIPFSFVRLGAYNRIVKSA-WKESEGWNLDHNLENGLNEKCLKTLRIMGI---GGYRCSKLR---TDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGP-EPFSQFLPIGLVLAQTY-QTKSGKFIPNHQVKYLDDKP------VYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVN--------------SPVNDPKVFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSL 2365
W++K ++ + + D+ N YIL FPYPSG LH+GH+RVY +D L R + + VI PMGWDAFGLPAENAA++R P WT NI++MK+QM + M + DW RE++TCDP YYK+TQW+F +LFE G Y+K +NWDP+D TVLA+E +D GRSWRSGAIVEK LKQWF+ T + L L+ +K W V + QK WIG+ G + F + D + EN I VFT P + V +V + D+ I Y E+ E + L IK +PL+ +PI + S+ +G P D +D T + S V RI+ + + ++G E+ + IMG+ G S +R DWLISRQRYWGTPIPI+HC +CGPV P++ LPV LP + + I ++ CP CG+P A RE+DTMDTF+DSSWY+ R+LD KN+ + S++MPVDIYIGG+EHAI HL Y+RF++ F+ P G EPF + + G+V +TY SGKF+ ++ +++D P + + + P+ + +KMSKSK+NG P + ++G D TR IL + W E+ IV GI+ W ++V +L K I++ + +ND +V NDF Q + I ++ + +++ V+S KLT++
Sbjct: 30 WKQKTTRGLPKQDTLNSGSKYILCQFPYPSGALHIGHLRVYVISDSLNRFYKQKGYNVIHPMGWDAFGLPAENAAIERSINPAIWTRDNIAKMKQQM-QSMLANFDWDREITTCDPEYYKFTQWIFLKLFENGLAYRKEAEINWDPVDMTVLANEQVDAQGRSWRSGAIVEKKQLKQWFLGITKFAPKLKKHLNQLK--DWPSNVKQMQKNWIGESVGAELVFKVADPKFENLI-----VFTTRPETLFA--VQYVALALDHPIVQKYCEEMPDLKEFIQKSDQLPNDTKEGFQLPNIKAVNPLTKEEVPIFAAPYVVSSYGSAPSAVMGCPGHDNRDFEFWQTNCPGEHIKTCIAPFFDDASKVTEQERQRIIDTVPFTSTDGVLTKECGEHSGVLTVVARKSIMGMLNSEGLSKSVVRYKIRDWLISRQRYWGTPIPIIHCDNCGPVPVPESDLPVKLPELEGLDTKGNPLSTID------EFVNVACPSCGSP-AKRETDTMDTFIDSSWYYFRFLDPKNTSKPFDREIASKNMPVDIYIGGVEHAILHLLYSRFIAKFLGSINAWSDPAGIFEPFKKLVTQGMVQGKTYVDPDSGKFLKPDELTFVNDSPDGNTVIIKSNGKVPVVSY-EKMSKSKYNGADPNECILRHGPDATRAHILFQSPIADALNWDESKIV-GIERWLQKVLHLTKNILSLEKDLAISKDYKTPTDLNDAEVKFHNDF-----------QRFLKSITESFEVNLSLNTVISDYMKLTNI 767
BLAST of Leucine--tRNA ligase vs. Ensembl Yeast
Match: VAS1 (Mitochondrial and cytoplasmic valyl-tRNA synthetase; human homolog VARS2 implicated in mitochondrial diseases, can partially complement yeast null mutant [Source:SGD;Acc:S000003326]) HSP 1 Score: 64.3142 bits (155), Expect = 6.012e-11 Identity = 43/150 (28.67%), Postives = 70/150 (46.67%), Query Frame = 2
Query: 311 PYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLP----------AENAALQRGYEPE-------KWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDN 709
P +G LH+GH D L R NR+ V+F G+D G+ A++ + Y E +W + S +K Q+ +++ S DWSRE T P K + F RL ++G +Y+ S +VNW T +++ ++N
Sbjct: 190 PNVTGALHIGHALTIAIQDSLIRYNRMKGKTVLFLPGFDHAGIATQSVVEKQIWAKDRKTRHDYGREAFVGKVWEWKEEYHSRIKNQI-QKLGASYDWSREAFTLSPELTKSVEEAFVRLHDEGVIYRASRLVNWSVKLNTAISNLEVEN 338
BLAST of Leucine--tRNA ligase vs. Ensembl Yeast
Match: ISM1 (Mitochondrial isoleucyl-tRNA synthetase; null mutant is deficient in respiratory growth; human homolog IARS2 implicated in mitochondrial diseases, can partially complement yeast null mutant [Source:SGD;Acc:S000005961]) HSP 1 Score: 60.8474 bits (146), Expect = 8.655e-10 Identity = 51/200 (25.50%), Postives = 90/200 (45.00%), Query Frame = 2
Query: 215 KKQLETFWQEKMSKITQNDSA---NRKKMYIL-SMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQ-------RGYEPEKWTLKNISEMKEQMKEQ------MTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQ 763
K+QL F++E SK+ D ++K++IL PY +G+LH+GH D++ R + + GWD GLP E AL+ P K + ++ +K Q + DW T D +Y +F ++E+G + ++++ V W +T LA+ ++ + +S A K PL++
Sbjct: 59 KEQLRDFFEE-FSKLNTTDEKLEFIKEKLFILHDGPPYANGELHLGHALNKILKDIINRYQLSQGKYIFYKPGWDCHGLPIEIKALKDLSAQQIESISPLKIRSMALKHAQKAIKRQRETFQHFAILTDWETPYLTMDKDYEINQLNIFKEMYERGLIKRQNKPVYWGTETRTALAEGELE-YNENHKSIAAYVKFPLEK 256
BLAST of Leucine--tRNA ligase vs. Ensembl Yeast
Match: ILS1 (Cytoplasmic isoleucine-tRNA synthetase; target of the G1-specific inhibitor reveromycin A [Source:SGD;Acc:S000000172]) HSP 1 Score: 50.0618 bits (118), Expect = 1.718e-6 Identity = 35/136 (25.74%), Postives = 60/136 (44.12%), Query Frame = 2
Query: 311 PYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENA-----ALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELS-------------TCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNW 664
P+ +G H GH+ T D++ R + H V GWD G+P E+ + + K+ L+N + E MT + DW + + T P++ + T W F +L EKG VY+ +++ +
Sbjct: 47 PFATGTPHYGHILASTIKDIVPRYATMTGHHVERRFGWDTHGVPIEHIIDKKLGITGKDDVFKYGLENYN--NECRSIVMTYASDWRKTIGRLGRWIDFDNDYKTMYPSFMESTWWAFKQLHEKGQVYRGFKVMPY 180
BLAST of Leucine--tRNA ligase vs. Ensembl Nematostella
Match: EDO29248 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T464]) HSP 1 Score: 181.03 bits (458), Expect = 6.950e-50 Identity = 125/382 (32.72%), Postives = 187/382 (48.95%), Query Frame = 2
Query: 1334 EKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPS---------VVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNH----QVKYLDDK--PVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLVNDFLLQDSEFIAKYQSL-------VTKILFQYQKSFTISVVVSVLQKLTSL--YMKFSNEKAKSSIF 2407
+K + L G+G + + DW +SRQRYWGTPIPI+HC CG P LPV+LP S S+AF+ CP CG A RE+DTMDTFVDSSWYF+RY D++NS+ + + + +D YIGG+EHAI HL YARF + + D+ L EPF++ L G+VL Y ++ K + V+++ D PV D E MSKSK NGI P+ +++KYG D RL + P+ +W + V G + RRV+N ++ + + L D EF +SL + ++ + YQ+ +VV ++ + +L + F + A+ ++
Sbjct: 11 DKVAELLAAKGLGEKKTTWRLRDWGVSRQRYWGTPIPIIHCEDCGAQPVPAKDLPVVLP----QDLVPDGSGNPLIKSEAFH-AGVVCPCCGK-SARRETDTMDTFVDSSWYFMRYCDARNSEQMVAEGAEYWMRDQNAATGGSGMDQYIGGIEHAILHLLYARFWTKVMR---DLGLVKVDEPFTKLLTQGMVLNHIYSRRTAKGAKEYFWPKDVEHVADGLLPVGTDID---YEGVGTMSKSKNNGIDPQELIEKYGADTARLYTMFTAPPELTLEWNDA-AVEGSYRFLRRVYNFGAKLSQMDMAGAVQSVAGAKSLDDVEFGKAAKSLRLEIHTVLKQVEYDYQRMQYNTVVSGAMKMINALEDFKSFDDAGAQVALI 379
BLAST of Leucine--tRNA ligase vs. Ensembl Nematostella
Match: EDO26794 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TB22]) HSP 1 Score: 63.5438 bits (153), Expect = 2.924e-10 Identity = 42/147 (28.57%), Postives = 69/147 (46.94%), Query Frame = 2
Query: 311 PYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAE----NAALQRGYEPE------------KWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELI 703
P +G LHMGH T D+L R ++ + + G D + E N +RG + +W K + +Q+K ++ ++DW+R T D YY +F L+ KG +Y+ +++NWDP KT L+DE +
Sbjct: 43 PNVTGVLHMGHALNDTVQDILVRRAKMQGYNTCWVPGTDHASIATEAKVVNMLKERGIDKNALSREEFLEYAWEWKEKYGGIILQQLK-KLGCALDWNRTHFTMDDEYYAAVIRVFVDLYNKGQIYRGVKMINWDPKAKTALSDEEV 188
BLAST of Leucine--tRNA ligase vs. Ensembl Nematostella
Match: EDO25681 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8DVS8]) HSP 1 Score: 55.8398 bits (133), Expect = 2.745e-8 Identity = 40/147 (27.21%), Postives = 67/147 (45.58%), Query Frame = 2
Query: 278 NRKKMYILSMFPYPSGQLHMGHMR-VYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKG-FVYQKSEIVNWDPIDKTVLADELIDNT 712
N K+ I + PY +G +H+GH+ VY +D+ AR RL +V F G D G+ A + G P++ K +++ + I + T ++ Q F +L+E G F+ Q +E + +D LAD + T
Sbjct: 4 NPKRYTITAALPYTNGPIHIGHLAGVYVPSDIYARYLRLQGKDVAFMCGSDEHGVAISMKAKKEGITPQEVIDKYDGIIRQSF---LDFGISFDNYSRTSSKIHHDTAQEFFRKLYENGKFIEQTTEQL-YDAKADQFLADRFVTGT 146
BLAST of Leucine--tRNA ligase vs. Ensembl Medaka
Match: lars2 (leucyl-tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:101175189]) HSP 1 Score: 491.115 bits (1263), Expect = 2.729e-159 Identity = 296/820 (36.10%), Postives = 448/820 (54.63%), Query Frame = 2
Query: 122 LNSSRYVALKHVK-FFSCSMCFDIMPHYDFNVKKQLETFWQEKMSKITQNDS----ANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEI--------NNDNIWWNYXXXXXXXXXXLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQ-TKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQ-ENDIVLGIQNWERRVWNLIKEIVNS----PVNDPKVFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFXXXXXXXXXXXXXXXWAQLASKLAPIESIFI 2524
L R + H + FS + +D Y + ++E +W ++ Q D+ + +KK Y+LSMFPYPSG+LHMGH+RVYT +D + R+ HEV+ PMGWDAFGLPAENAA++R +PE WT NI M+ Q+ + + L +W RE++TC P+YYKWTQ+LF +LFE G YQK +VNWDP+D+TVLADE +D +GRSWRSGA+VE+ L QWFI+TT ++ LLD L+ + +W V Q WIG+ TG Y D+ L E E LS ++ P A+V I + ++ + + + L+ +L + +GR +P++ ++ F + + +G+P +D ++A L + ++ V L + ++ + L E + K R +GG+ S DWLISRQRYWGTPIP+VHC SCG V P+ +LPV LP K + SPL A +W+ +CPKC P A RE+DTMDTFVDS+WY+ RY D N D + +PVD+YIGG EHA+ HLYYARF+SHF D L EPF + L GL+ QT++ G+++ ++ + +PV + P+ W+KMSKSK NG+ P+ VV +YG+D RL IL P+++ W + D + G+ W+ R+W L+ ++ + V +P + + L + + + ++ + + F + +S L LT+ + S+ + L ++ P++P L+SELWA L P+ S+ +
Sbjct: 60 LGPVRRLRTPHTRTLFSETGVWDT--DYRSETRCRVERWWHPRVMAQWQKDALEENSQKKKFYVLSMFPYPSGRLHMGHVRVYTISDAIGHFQRMRGHEVLNPMGWDAFGLPAENAAIERNLDPEDWTKSNIQSMRGQL-DDLGLCFNWDREVTTCLPDYYKWTQYLFIKLFESGLAYQKEAVVNWDPVDRTVLADEQVDESGRSWRSGALVEQKLLTQWFIKTTNYAKPLLDALEELP--EWYGVKAMQANWIGECTGCYFDYRLKVNGKETG--ETLSAYSSSPEVVFG--AAYVAILPSHRLLHGSSSVRFALLKSFQSGTDCLTEVTALNL---FTGREVPLLISRKNMFDGY--LDTVIGVPECSEEDLSVAKALNLSWTTV-LETHENGAQTLINSDKFSGLTR--EQAFDSITQKA-REQKVGGHLTSSKLRDWLISRQRYWGTPIPVVHCESCGAVTVPEEELPVALP-----KVPSLTGKGASPLEAAHDWVNCKCPKCKGP-ARRETDTMDTFVDSAWYYFRYTDPHNPDRPFRRHLADHWLPVDVYIGGKEHAVMHLYYARFLSHFCR---DQGLVAHREPFRKLLVQGLIKGQTFKLADGGQYLRKEEIDFTGKEPVALSGG-PVEVTWEKMSKSKHNGLDPQEVVQQYGVDTVRLYILYAAPPEQDILWNVKTDALPGVLRWQSRLWQLVTKLRGARELGDVPNPSLLKTKE-LAEAKKIWENKNYAIQEVTAHFTEDFLFNAAISRLMGLTNTLTSAQVGVLQHSVEFEEALAALVLMTAPMAPHLASELWAGLCQIKNPLSSVLL 850
BLAST of Leucine--tRNA ligase vs. Ensembl Medaka
Match: vars2 (valyl-tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:101172991]) HSP 1 Score: 58.5362 bits (140), Expect = 3.044e-8 Identity = 40/150 (26.67%), Postives = 66/150 (44.00%), Query Frame = 2
Query: 311 PYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGY------------------EPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELID 706
P +G LH+GH D L R R+ V++ G D G+ A A ++R E KW + E+ +Q++ ++ S+DWSR T DP + F RL + G +Y+ +VNW ++ ++D +D
Sbjct: 148 PNVTGSLHLGHALTVAVEDALVRWRRMQGCRVLWVPGCDHAGI-ATQAVVERRLVGTKGKRRQDFTRDEFLQEVWKWKNEKGDEIYQQLR-RLGASLDWSRARFTMDPGFCSTVTEAFVRLCDSGLIYRSEGLVNWSCALQSAISDIEVD 295
BLAST of Leucine--tRNA ligase vs. Ensembl Medaka
Match: iars2 (isoleucyl-tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:101171455]) HSP 1 Score: 57.7658 bits (138), Expect = 5.265e-8 Identity = 43/155 (27.74%), Postives = 65/155 (41.94%), Query Frame = 2
Query: 263 QNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAAL----QRGYEP-------EKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLAD 694
Q + +KK + PY +G H+GH D+ R L +V + GWD GLP E AL G P ++ I++ K + ++ DW + T D Y +F ++ KG VYQ + V W P +T LA+
Sbjct: 94 QRERKAKKKFSLHDGPPYANGAPHVGHALNKILKDIRNRFEVLRGRQVHYVPGWDCHGLPIELKALGGLCASGLTPLQIRQRAREFAEGAIAQQKAAFQRWGVMA-DWEQCYYTFDGPYEAAQLKVFQEMYSKGLVYQDYKPVFWSPSSRTALAE 247
BLAST of Leucine--tRNA ligase vs. Ensembl Medaka
Match: iars2 (isoleucyl-tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:101171455]) HSP 1 Score: 57.7658 bits (138), Expect = 5.442e-8 Identity = 43/155 (27.74%), Postives = 65/155 (41.94%), Query Frame = 2
Query: 263 QNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAAL----QRGYEP-------EKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLAD 694
Q + +KK + PY +G H+GH D+ R L +V + GWD GLP E AL G P ++ I++ K + ++ DW + T D Y +F ++ KG VYQ + V W P +T LA+
Sbjct: 94 QRERKAKKKFSLHDGPPYANGAPHVGHALNKILKDIRNRFEVLRGRQVHYVPGWDCHGLPIELKALGGLCASGLTPLQIRQRAREFAEGAIAQQKAAFQRWGVMA-DWEQCYYTFDGPYEAAQLKVFQEMYSKGLVYQDYKPVFWSPSSRTALAE 247
BLAST of Leucine--tRNA ligase vs. Ensembl Medaka
Match: iars2 (isoleucyl-tRNA synthetase 2, mitochondrial [Source:NCBI gene;Acc:101171455]) HSP 1 Score: 57.7658 bits (138), Expect = 5.856e-8 Identity = 43/155 (27.74%), Postives = 65/155 (41.94%), Query Frame = 2
Query: 263 QNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAAL----QRGYEP-------EKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLAD 694
Q + +KK + PY +G H+GH D+ R L +V + GWD GLP E AL G P ++ I++ K + ++ DW + T D Y +F ++ KG VYQ + V W P +T LA+
Sbjct: 94 QRERKAKKKFSLHDGPPYANGAPHVGHALNKILKDIRNRFEVLRGRQVHYVPGWDCHGLPIELKALGGLCASGLTPLQIRQRAREFAEGAIAQQKAAFQRWGVMA-DWEQCYYTFDGPYEAAQLKVFQEMYSKGLVYQDYKPVFWSPSSRTALAE 247
BLAST of Leucine--tRNA ligase vs. Planmine SMEST
Match: SMESG000071689.1 (SMESG000071689.1) HSP 1 Score: 1665.59 bits (4312), Expect = 0.000e+0 Identity = 849/850 (99.88%), Postives = 849/850 (99.88%), Query Frame = 2
Query: 110 MIKLLNSSRYVALKHVKFFSCSMCFDIMPHYDFNVKKQLETFWQEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFXXXXXXXXXXXXXXXWAQLASKLAPIESIFIETDDSYKSIFGPWGYNLNLPVWDQRLNVNEITLDIQNESVNIVNE 2659
MIKLLNSSRYVALKHVKFFSCSMCFDIMPHYDFNVKKQLETFWQEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSG IVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYKEDKSKSDEKLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYSECKSSESDSEKISPLSKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFLIYLYPLSPLLSSELWAQLASKLAPIESIFIETDDSYKSIFGPWGYNLNLPVWDQRLNVNEITLDIQNESVNIVNE
Sbjct: 1 MIKLLNSSRYVALKHVKFFSCSMCFDIMPHYDFNVKKQLETFWQEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGVIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYKEDKSKSDEKLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYSECKSSESDSEKISPLSKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFLIYLYPLSPLLSSELWAQLASKLAPIESIFIETDDSYKSIFGPWGYNLNLPVWDQRLNVNEITLDIQNESVNIVNE 850
BLAST of Leucine--tRNA ligase vs. Planmine SMEST
Match: SMESG000071689.1 (SMESG000071689.1) HSP 1 Score: 1660.97 bits (4300), Expect = 0.000e+0 Identity = 847/851 (99.53%), Postives = 847/851 (99.53%), Query Frame = 2
Query: 110 MIKLLNSSRYVALKHVKFFSCSMCFDIMPHYDFNVKKQLETFWQEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXX-XXLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFXXXXXXXXXXXXXXXWAQLASKLAPIESIFIETDDSYKSIFGPWGYNLNLPVWDQRLNVNEITLDIQNESVNIVNE 2659
MIKLLNSSRYVALKHVKFFSCSMCFDIMPHYDFNVKKQLETFWQEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSG IVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYKEDKSK EKLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYSECKSSESDSEKISPLSKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFLIYLYPLSPLLSSELWAQLASKLAPIESIFIETDDSYKSIFGPWGYNLNLPVWDQRLNVNEITLDIQNESVNIVNE
Sbjct: 1 MIKLLNSSRYVALKHVKFFSCSMCFDIMPHYDFNVKKQLETFWQEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGVIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYKEDKSKQSDEKLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYSECKSSESDSEKISPLSKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFLIYLYPLSPLLSSELWAQLASKLAPIESIFIETDDSYKSIFGPWGYNLNLPVWDQRLNVNEITLDIQNESVNIVNE 851
BLAST of Leucine--tRNA ligase vs. Planmine SMEST
Match: SMESG000071689.1 (SMESG000071689.1) HSP 1 Score: 1615.9 bits (4183), Expect = 0.000e+0 Identity = 825/829 (99.52%), Postives = 825/829 (99.52%), Query Frame = 2
Query: 176 MCFDIMPHYDFNVKKQLETFWQEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXX-XXLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFXXXXXXXXXXXXXXXWAQLASKLAPIESIFIETDDSYKSIFGPWGYNLNLPVWDQRLNVNEITLDIQNESVNIVNE 2659
MCFDIMPHYDFNVKKQLETFWQEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSG IVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYKEDKSK EKLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYSECKSSESDSEKISPLSKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFLIYLYPLSPLLSSELWAQLASKLAPIESIFIETDDSYKSIFGPWGYNLNLPVWDQRLNVNEITLDIQNESVNIVNE
Sbjct: 1 MCFDIMPHYDFNVKKQLETFWQEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGVIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYKEDKSKQSDEKLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYSECKSSESDSEKISPLSKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFLIYLYPLSPLLSSELWAQLASKLAPIESIFIETDDSYKSIFGPWGYNLNLPVWDQRLNVNEITLDIQNESVNIVNE 829
BLAST of Leucine--tRNA ligase vs. Planmine SMEST
Match: SMESG000071689.1 (SMESG000071689.1) HSP 1 Score: 1608.96 bits (4165), Expect = 0.000e+0 Identity = 822/823 (99.88%), Postives = 822/823 (99.88%), Query Frame = 2
Query: 191 MPHYDFNVKKQLETFWQEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXXXXLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFXXXXXXXXXXXXXXXWAQLASKLAPIESIFIETDDSYKSIFGPWGYNLNLPVWDQRLNVNEITLDIQNESVNIVNE 2659
MPHYDFNVKKQLETFWQEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSG IVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYKEDKSKSDEKLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYSECKSSESDSEKISPLSKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFLIYLYPLSPLLSSELWAQLASKLAPIESIFIETDDSYKSIFGPWGYNLNLPVWDQRLNVNEITLDIQNESVNIVNE
Sbjct: 1 MPHYDFNVKKQLETFWQEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGVIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYKEDKSKSDEKLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYSECKSSESDSEKISPLSKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFLIYLYPLSPLLSSELWAQLASKLAPIESIFIETDDSYKSIFGPWGYNLNLPVWDQRLNVNEITLDIQNESVNIVNE 823
BLAST of Leucine--tRNA ligase vs. Planmine SMEST
Match: SMESG000071689.1 (SMESG000071689.1) HSP 1 Score: 1604.73 bits (4154), Expect = 0.000e+0 Identity = 820/824 (99.51%), Postives = 820/824 (99.51%), Query Frame = 2
Query: 191 MPHYDFNVKKQLETFWQEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGAIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYXXXXXXXX-XXLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYXXXXXXXXXXXXXXXXXKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFXXXXXXXXXXXXXXXWAQLASKLAPIESIFIETDDSYKSIFGPWGYNLNLPVWDQRLNVNEITLDIQNESVNIVNE 2659
MPHYDFNVKKQLETFWQEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSG IVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYKEDKSK EKLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYSECKSSESDSEKISPLSKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFLIYLYPLSPLLSSELWAQLASKLAPIESIFIETDDSYKSIFGPWGYNLNLPVWDQRLNVNEITLDIQNESVNIVNE
Sbjct: 1 MPHYDFNVKKQLETFWQEKMSKITQNDSANRKKMYILSMFPYPSGQLHMGHMRVYTTADVLARVNRLMNHEVIFPMGWDAFGLPAENAALQRGYEPEKWTLKNISEMKEQMKEQMTLSIDWSRELSTCDPNYYKWTQWLFCRLFEKGFVYQKSEIVNWDPIDKTVLADELIDNTGRSWRSGVIVEKVPLKQWFIRTTALSESLLDGLDTVKGGQWRRVIEAQKGWIGDLTGVYIDFDLVDFQDENRILEKLSVFTEHPVAALSDFVAFVEINNDNIWWNYKEDKSKQSDEKLSFHRSLAIKHPLSGRLIPIIRNQNLKFQSQDCVSSRLGLPLVDFKDATLASKLGIPFSFVRLGAYNRIVKSAWKESEGWNLDHNLENGLNEKCLKTLRIMGIGGYRCSKLRTDWLISRQRYWGTPIPIVHCPSCGPVLTPDNQLPVMLPPYSECKSSESDSEKISPLSKAFNWMKSECPKCGNPEATRESDTMDTFVDSSWYFLRYLDSKNSDYLCSPSVVSEHMPVDIYIGGMEHAIRHLYYARFMSHFIHYYTDIKLPNGPEPFSQFLPIGLVLAQTYQTKSGKFIPNHQVKYLDDKPVYIDNQEPLSEFWDKMSKSKFNGISPKTVVDKYGLDMTRLIILANTGPDKNRKWQENDIVLGIQNWERRVWNLIKEIVNSPVNDPKVFLVNDFLLQDSEFIAKYQSLVTKILFQYQKSFTISVVVSVLQKLTSLYMKFSNEKAKSSIFYHRMLLDFLIYLYPLSPLLSSELWAQLASKLAPIESIFIETDDSYKSIFGPWGYNLNLPVWDQRLNVNEITLDIQNESVNIVNE 824 The following BLAST results are available for this feature:
BLAST of Leucine--tRNA ligase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Leucine--tRNA ligase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Leucine--tRNA ligase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Leucine--tRNA ligase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Leucine--tRNA ligase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Leucine--tRNA ligase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Leucine--tRNA ligase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Leucine--tRNA ligase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Leucine--tRNA ligase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Leucine--tRNA ligase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 2
BLAST of Leucine--tRNA ligase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 4
BLAST of Leucine--tRNA ligase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 3
BLAST of Leucine--tRNA ligase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Leucine--tRNA ligase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30012572 ID=SMED30012572|Name=Leucine--tRNA ligase|organism=Schmidtea mediterranea sexual|type=transcript|length=2670bpback to top protein sequence of SMED30012572-orf-1 >SMED30012572-orf-1 ID=SMED30012572-orf-1|Name=SMED30012572-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=851bp MIKLLNSSRYVALKHVKFFSCSMCFDIMPHYDFNVKKQLETFWQEKMSKIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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