SMED30012483

Overview
NameSMED30012483
Smed IDSMED30012483
Length (bp)3607
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30012483 (SMED30012483) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30012483 (SMED30012483) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30012483 (SMED30012483) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30012483 (SMED30012483) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012483

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30012483SMESG000044609.1 dd_Smed_v4_1665_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30012483SMESG000044609.1 dd_Smed_v4_1665_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30012483SMESG000044609.1 dd_Smed_v4_1665_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30012483SMESG000044609.1 dd_Smed_v4_1665_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
neoblastSMED30012483SMESG000044609.1 dd_Smed_v4_1665_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30012483SMESG000044609.1 dd_Smed_v4_1665_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30012483SMESG000044609.1 dd_Smed_v4_1665_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012483SMESG000044609.1 Contig36516uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012483SMESG000044609.1 Contig36516newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012483SMESG000044609.1 Contig37842uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012483SMESG000044609.1 Contig37842newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30012483 vs. Planmine SMEST
Match: SMESG000044609.1 (SMESG000044609.1)

HSP 1 Score: 570.852 bits (1470), Expect = 0.000e+0
Identity = 360/378 (95.24%), Postives = 361/378 (95.50%), Query Frame = 1 
Query: 1522 PDNFLNPQNRKTKQILNRNSLLGGVPKPIDISKKIDAIKESQKKAAEAHSLSNLNIXXXXXXXXXXXXXXXXXXWCPSMDFGGPVAKKAKKTAGQKMTVENKPGAESNEMNSGSENHLVDYNYIGPQVSSDMAKKLGLDVKATKKQEKENSKKGGTEHTLGDSYLTAALDANHLALQSRAYQHRLNNLQGHNNSDFGGQTAQYFQSLYMSPXXXXXXXXXXXXXXXXXEQLPASVAGSLAQAAVQRIMSSSNLTATYNTPSVVATPTASYQSALQHTLPGSCSTLVASTPSGLVPISLLQQGGVSYGINXXXXXXXXXXXXXXXXXXXITIPNQSILGGXXXXXXXXXXXXXXXXXSFGFGAQPQLLASPLNFQHTLL 2655
            PDNFLNPQNRKTKQILNRNSLLGGVPKPIDISKKIDAIKESQKKAAEAHSLSNLNIPLPPEKPKTTPMKPGPGEWCPSMDFGGPVAKKAKKTAGQKMTVENKPGAESNEMNSGSENHLVDYNYIGPQVS                 +KENSKKGGTEHTLGDSYLTAALDANHLALQSRAYQHRLNNLQGHNNSDFGGQTAQYFQSLYMSPSSLTNNLTATALAAVASEQLPASVAGSLAQAAVQRIMSSSNLTATYNTPSVVATPTASYQSALQHTLPGSCSTLVASTPSGLVPISLLQQGGVSYGINQLVLQHLQQQALSAQLGQAITIPNQSILGGLNPLLIQQAQNQQALLQSFGFGAQPQLLASPLNFQHTLL
Sbjct:  474 PDNFLNPQNRKTKQILNRNSLLGGVPKPIDISKKIDAIKESQKKAAEAHSLSNLNIPLPPEKPKTTPMKPGPGEWCPSMDFGGPVAKKAKKTAGQKMTVENKPGAESNEMNSGSENHLVDYNYIGPQVS-----------------KKENSKKGGTEHTLGDSYLTAALDANHLALQSRAYQHRLNNLQGHNNSDFGGQTAQYFQSLYMSPSSLTNNLTATALAAVASEQLPASVAGSLAQAAVQRIMSSSNLTATYNTPSVVATPTASYQSALQHTLPGSCSTLVASTPSGLVPISLLQQGGVSYGINQLVLQHLQQQALSAQLGQAITIPNQSILGGLNPLLIQQAQNQQALLQSFGFGAQPQLLASPLNFQHTLL 834

HSP 2 Score: 398.282 bits (1022), Expect = 2.898e-156
Identity = 269/275 (97.82%), Postives = 274/275 (99.64%), Query Frame = 1 
Query:  169 NTSLKPISDKNGMLCPKSVRNQLXXXXXXXXXXXXXXXXXXXXXXXTNENSNSVIVSKKPILNLSDGKILEIKINKDKINFSNELSKSIKSEPNLNSINAIVTTSNHKTDESDQSIXXXXXXXXXXXXNAIXXXXXXXXXXXXXXXXXXXXXXXXISPEKLSIKDELHDVSVAKETPTADVKINKAPMSLLVSYSSDEEESHNEPQTKVIDIKISPEIKTEAIIKEHADNKIEIKIKPTKSPGKSAWDSSDSELENVAKETNIPFVKSRSPSAAI 993
            NTSLKPISDKNGMLCPKSVRNQLKKVKESSEKDDKKVDSKDGIKIKTNENSNSV+VSKKPILNLSDGKILEIKINKDKINFSNELSKSIKSEPN+NSINAIVTTSNHKTDESDQSIKRKKKKKHGKSKNAIDSDDGNKDGKNKK+RKSKKKKHKDISPE +SIKDELHDVSVAKETPTAD+KINKAPMSLLVSYSSDEEESHNEPQTKVIDIKISPEIKTEAIIKEHADNKIEIKIKPTKSPGKSAWDSSDSELENVAKETNIPFVKSRSPSAAI
Sbjct:   36 NTSLKPISDKNGMLCPKSVRNQLKKVKESSEKDDKKVDSKDGIKIKTNENSNSVVVSKKPILNLSDGKILEIKINKDKINFSNELSKSIKSEPNINSINAIVTTSNHKTDESDQSIKRKKKKKHGKSKNAIDSDDGNKDGKNKKTRKSKKKKHKDISPENISIKDELHDVSVAKETPTADIKINKAPMSLLVSYSSDEEESHNEPQTKVIDIKISPEIKTEAIIKEHADNKIEIKIKPTKSPGKSAWDSSDSELENVAKETNIPFVKSRSPSAAI 310

HSP 3 Score: 175.637 bits (444), Expect = 2.898e-156
Identity = 83/83 (100.00%), Postives = 83/83 (100.00%), Query Frame = 2 
Query: 1085 HIAEVEVNQVAILEVKQTVGQVNLVPVQVTHEVGHIAGLIVNHTQNHQIVDLDHILVRDRDQKGNQDAIGDILLLTVVHARDI 1333
            HIAEVEVNQVAILEVKQTVGQVNLVPVQVTHEVGHIAGLIVNHTQNHQIVDLDHILVRDRDQKGNQDAIGDILLLTVVHARDI
Sbjct:  341 HIAEVEVNQVAILEVKQTVGQVNLVPVQVTHEVGHIAGLIVNHTQNHQIVDLDHILVRDRDQKGNQDAIGDILLLTVVHARDI 423

HSP 4 Score: 180.644 bits (457), Expect = 6.148e-46
Identity = 121/140 (86.43%), Postives = 121/140 (86.43%), Query Frame = 1 
Query: 2857 DQFSEQLAKAEAEQQNQSSQVQISAGDESKALNIGDYQMVSEQQQMASSLHSINN-ESTTNLITSSTTELNQFEESAVNTTVTSEPSSETILFXXXXXXXXXXXXXXXXXXXGVIKQAPRIIYNSPAVMASLVTTPITKP 3273
            DQFSEQLAKAEAEQQNQ                  DYQMVSEQQQMASSLHSINN ESTTNLITSSTTELNQFEESAVNTTVTSEPSSETILFNSESEIESISNDISSSSSNGVIKQAPRIIYNSPAVMASLVTTPITKP
Sbjct:  902 DQFSEQLAKAEAEQQNQR-----------------DYQMVSEQQQMASSLHSINNNESTTNLITSSTTELNQFEESAVNTTVTSEPSSETILFNSESEIESISNDISSSSSNGVIKQAPRIIYNSPAVMASLVTTPITKP 1024
[Close]
BLAST of SMED30012483 vs. Planmine SMEST
Match: SMESG000044609.1 (SMESG000044609.1)

HSP 1 Score: 551.592 bits (1420), Expect = 5.269e-180
Identity = 352/370 (95.14%), Postives = 353/370 (95.41%), Query Frame = 1 
Query: 1522 PDNFLNPQNRKTKQILNRNSLLGGVPKPIDISKKIDAIKESQKKAAEAHSLSNLNIXXXXXXXXXXXXXXXXXXWCPSMDFGGPVAKKAKKTAGQKMTVENKPGAESNEMNSGSENHLVDYNYIGPQVSSDMAKKLGLDVKATKKQEKENSKKGGTEHTLGDSYLTAALDANHLALQSRAYQHRLNNLQGHNNSDFGGQTAQYFQSLYMSPXXXXXXXXXXXXXXXXXEQLPASVAGSLAQAAVQRIMSSSNLTATYNTPSVVATPTASYQSALQHTLPGSCSTLVASTPSGLVPISLLQQGGVSYGINXXXXXXXXXXXXXXXXXXXITIPNQSILGGXXXXXXXXXXXXXXXXXSFGFGAQPQLLASP 2631
            PDNFLNPQNRKTKQILNRNSLLGGVPKPIDISKKIDAIKESQKKAAEAHSLSNLNIPLPPEKPKTTPMKPGPGEWCPSMDFGGPVAKKAKKTAGQKMTVENKPGAESNEMNSGSENHLVDYNYIGPQVS                 +KENSKKGGTEHTLGDSYLTAALDANHLALQSRAYQHRLNNLQGHNNSDFGGQTAQYFQSLYMSPSSLTNNLTATALAAVASEQLPASVAGSLAQAAVQRIMSSSNLTATYNTPSVVATPTASYQSALQHTLPGSCSTLVASTPSGLVPISLLQQGGVSYGINQLVLQHLQQQALSAQLGQAITIPNQSILGGLNPLLIQQAQNQQALLQSFGFGAQPQLLASP
Sbjct:  480 PDNFLNPQNRKTKQILNRNSLLGGVPKPIDISKKIDAIKESQKKAAEAHSLSNLNIPLPPEKPKTTPMKPGPGEWCPSMDFGGPVAKKAKKTAGQKMTVENKPGAESNEMNSGSENHLVDYNYIGPQVS-----------------KKENSKKGGTEHTLGDSYLTAALDANHLALQSRAYQHRLNNLQGHNNSDFGGQTAQYFQSLYMSPSSLTNNLTATALAAVASEQLPASVAGSLAQAAVQRIMSSSNLTATYNTPSVVATPTASYQSALQHTLPGSCSTLVASTPSGLVPISLLQQGGVSYGINQLVLQHLQQQALSAQLGQAITIPNQSILGGLNPLLIQQAQNQQALLQSFGFGAQPQLLASP 832

HSP 2 Score: 395.971 bits (1016), Expect = 3.462e-155
Identity = 269/275 (97.82%), Postives = 274/275 (99.64%), Query Frame = 1 
Query:  169 NTSLKPISDKNGMLCPKSVRNQLXXXXXXXXXXXXXXXXXXXXXXXTNENSNSVIVSKKPILNLSDGKILEIKINKDKINFSNELSKSIKSEPNLNSINAIVTTSNHKTDESDQSIXXXXXXXXXXXXNAIXXXXXXXXXXXXXXXXXXXXXXXXISPEKLSIKDELHDVSVAKETPTADVKINKAPMSLLVSYSSDEEESHNEPQTKVIDIKISPEIKTEAIIKEHADNKIEIKIKPTKSPGKSAWDSSDSELENVAKETNIPFVKSRSPSAAI 993
            NTSLKPISDKNGMLCPKSVRNQLKKVKESSEKDDKKVDSKDGIKIKTNENSNSV+VSKKPILNLSDGKILEIKINKDKINFSNELSKSIKSEPN+NSINAIVTTSNHKTDESDQSIKRKKKKKHGKSKNAIDSDDGNKDGKNKK+RKSKKKKHKDISPE +SIKDELHDVSVAKETPTAD+KINKAPMSLLVSYSSDEEESHNEPQTKVIDIKISPEIKTEAIIKEHADNKIEIKIKPTKSPGKSAWDSSDSELENVAKETNIPFVKSRSPSAAI
Sbjct:   42 NTSLKPISDKNGMLCPKSVRNQLKKVKESSEKDDKKVDSKDGIKIKTNENSNSVVVSKKPILNLSDGKILEIKINKDKINFSNELSKSIKSEPNINSINAIVTTSNHKTDESDQSIKRKKKKKHGKSKNAIDSDDGNKDGKNKKTRKSKKKKHKDISPENISIKDELHDVSVAKETPTADIKINKAPMSLLVSYSSDEEESHNEPQTKVIDIKISPEIKTEAIIKEHADNKIEIKIKPTKSPGKSAWDSSDSELENVAKETNIPFVKSRSPSAAI 316

HSP 3 Score: 174.096 bits (440), Expect = 3.462e-155
Identity = 83/83 (100.00%), Postives = 83/83 (100.00%), Query Frame = 2 
Query: 1085 HIAEVEVNQVAILEVKQTVGQVNLVPVQVTHEVGHIAGLIVNHTQNHQIVDLDHILVRDRDQKGNQDAIGDILLLTVVHARDI 1333
            HIAEVEVNQVAILEVKQTVGQVNLVPVQVTHEVGHIAGLIVNHTQNHQIVDLDHILVRDRDQKGNQDAIGDILLLTVVHARDI
Sbjct:  347 HIAEVEVNQVAILEVKQTVGQVNLVPVQVTHEVGHIAGLIVNHTQNHQIVDLDHILVRDRDQKGNQDAIGDILLLTVVHARDI 429
[Close]
BLAST of SMED30012483 vs. Planmine SMEST
Match: SMESG000044609.1 (SMESG000044609.1)

HSP 1 Score: 551.206 bits (1419), Expect = 5.927e-180
Identity = 352/370 (95.14%), Postives = 353/370 (95.41%), Query Frame = 1 
Query: 1522 PDNFLNPQNRKTKQILNRNSLLGGVPKPIDISKKIDAIKESQKKAAEAHSLSNLNIXXXXXXXXXXXXXXXXXXWCPSMDFGGPVAKKAKKTAGQKMTVENKPGAESNEMNSGSENHLVDYNYIGPQVSSDMAKKLGLDVKATKKQEKENSKKGGTEHTLGDSYLTAALDANHLALQSRAYQHRLNNLQGHNNSDFGGQTAQYFQSLYMSPXXXXXXXXXXXXXXXXXEQLPASVAGSLAQAAVQRIMSSSNLTATYNTPSVVATPTASYQSALQHTLPGSCSTLVASTPSGLVPISLLQQGGVSYGINXXXXXXXXXXXXXXXXXXXITIPNQSILGGXXXXXXXXXXXXXXXXXSFGFGAQPQLLASP 2631
            PDNFLNPQNRKTKQILNRNSLLGGVPKPIDISKKIDAIKESQKKAAEAHSLSNLNIPLPPEKPKTTPMKPGPGEWCPSMDFGGPVAKKAKKTAGQKMTVENKPGAESNEMNSGSENHLVDYNYIGPQVS                 +KENSKKGGTEHTLGDSYLTAALDANHLALQSRAYQHRLNNLQGHNNSDFGGQTAQYFQSLYMSPSSLTNNLTATALAAVASEQLPASVAGSLAQAAVQRIMSSSNLTATYNTPSVVATPTASYQSALQHTLPGSCSTLVASTPSGLVPISLLQQGGVSYGINQLVLQHLQQQALSAQLGQAITIPNQSILGGLNPLLIQQAQNQQALLQSFGFGAQPQLLASP
Sbjct:  474 PDNFLNPQNRKTKQILNRNSLLGGVPKPIDISKKIDAIKESQKKAAEAHSLSNLNIPLPPEKPKTTPMKPGPGEWCPSMDFGGPVAKKAKKTAGQKMTVENKPGAESNEMNSGSENHLVDYNYIGPQVS-----------------KKENSKKGGTEHTLGDSYLTAALDANHLALQSRAYQHRLNNLQGHNNSDFGGQTAQYFQSLYMSPSSLTNNLTATALAAVASEQLPASVAGSLAQAAVQRIMSSSNLTATYNTPSVVATPTASYQSALQHTLPGSCSTLVASTPSGLVPISLLQQGGVSYGINQLVLQHLQQQALSAQLGQAITIPNQSILGGLNPLLIQQAQNQQALLQSFGFGAQPQLLASP 826

HSP 2 Score: 395.971 bits (1016), Expect = 3.056e-155
Identity = 269/275 (97.82%), Postives = 274/275 (99.64%), Query Frame = 1 
Query:  169 NTSLKPISDKNGMLCPKSVRNQLXXXXXXXXXXXXXXXXXXXXXXXTNENSNSVIVSKKPILNLSDGKILEIKINKDKINFSNELSKSIKSEPNLNSINAIVTTSNHKTDESDQSIXXXXXXXXXXXXNAIXXXXXXXXXXXXXXXXXXXXXXXXISPEKLSIKDELHDVSVAKETPTADVKINKAPMSLLVSYSSDEEESHNEPQTKVIDIKISPEIKTEAIIKEHADNKIEIKIKPTKSPGKSAWDSSDSELENVAKETNIPFVKSRSPSAAI 993
            NTSLKPISDKNGMLCPKSVRNQLKKVKESSEKDDKKVDSKDGIKIKTNENSNSV+VSKKPILNLSDGKILEIKINKDKINFSNELSKSIKSEPN+NSINAIVTTSNHKTDESDQSIKRKKKKKHGKSKNAIDSDDGNKDGKNKK+RKSKKKKHKDISPE +SIKDELHDVSVAKETPTAD+KINKAPMSLLVSYSSDEEESHNEPQTKVIDIKISPEIKTEAIIKEHADNKIEIKIKPTKSPGKSAWDSSDSELENVAKETNIPFVKSRSPSAAI
Sbjct:   36 NTSLKPISDKNGMLCPKSVRNQLKKVKESSEKDDKKVDSKDGIKIKTNENSNSVVVSKKPILNLSDGKILEIKINKDKINFSNELSKSIKSEPNINSINAIVTTSNHKTDESDQSIKRKKKKKHGKSKNAIDSDDGNKDGKNKKTRKSKKKKHKDISPENISIKDELHDVSVAKETPTADIKINKAPMSLLVSYSSDEEESHNEPQTKVIDIKISPEIKTEAIIKEHADNKIEIKIKPTKSPGKSAWDSSDSELENVAKETNIPFVKSRSPSAAI 310

HSP 3 Score: 174.481 bits (441), Expect = 3.056e-155
Identity = 83/83 (100.00%), Postives = 83/83 (100.00%), Query Frame = 2 
Query: 1085 HIAEVEVNQVAILEVKQTVGQVNLVPVQVTHEVGHIAGLIVNHTQNHQIVDLDHILVRDRDQKGNQDAIGDILLLTVVHARDI 1333
            HIAEVEVNQVAILEVKQTVGQVNLVPVQVTHEVGHIAGLIVNHTQNHQIVDLDHILVRDRDQKGNQDAIGDILLLTVVHARDI
Sbjct:  341 HIAEVEVNQVAILEVKQTVGQVNLVPVQVTHEVGHIAGLIVNHTQNHQIVDLDHILVRDRDQKGNQDAIGDILLLTVVHARDI 423
[Close]
BLAST of SMED30012483 vs. Planmine SMEST
Match: SMESG000044609.1 (SMESG000044609.1)

HSP 1 Score: 181.415 bits (459), Expect = 5.087e-52
Identity = 121/140 (86.43%), Postives = 121/140 (86.43%), Query Frame = 1 
Query: 2857 DQFSEQLAKAEAEQQNQSSQVQISAGDESKALNIGDYQMVSEQQQMASSLHSINN-ESTTNLITSSTTELNQFEESAVNTTVTSEPSSETILFXXXXXXXXXXXXXXXXXXXGVIKQAPRIIYNSPAVMASLVTTPITKP 3273
            DQFSEQLAKAEAEQQNQ                  DYQMVSEQQQMASSLHSINN ESTTNLITSSTTELNQFEESAVNTTVTSEPSSETILFNSESEIESISNDISSSSSNGVIKQAPRIIYNSPAVMASLVTTPITKP
Sbjct:   68 DQFSEQLAKAEAEQQNQ-----------------RDYQMVSEQQQMASSLHSINNNESTTNLITSSTTELNQFEESAVNTTVTSEPSSETILFNSESEIESISNDISSSSSNGVIKQAPRIIYNSPAVMASLVTTPITKP 190
The following BLAST results are available for this feature:
BLAST of SMED30012483 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012483 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012483 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012483 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012483 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012483 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012483 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012483 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012483 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012483 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012483 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012483 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012483 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012483 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000044609.12.898e-15695.24SMESG000044609.1[more]
SMESG000044609.15.269e-18095.14SMESG000044609.1[more]
SMESG000044609.15.927e-18095.14SMESG000044609.1[more]
SMESG000044609.15.087e-5286.43SMESG000044609.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012483 ID=SMED30012483|Name=SMED30012483|organism=Schmidtea mediterranea sexual|type=transcript|length=3607bp
ATTTATTTGCTTACATACTTTCGCCATTTATGATATAAATTTATTTCGAA
ATTATATGTTACTATGAAGAAGTCAGATGATAAGAAGCCAACCTCGGAAA
AGACTAAATCAAAATCTGATAAAGGAGCTGAATCAAAATCAGAGATAGAA
TCATCAGAGAAATTTAAAAATACATCCCTAAAACCCATAAGTGATAAAAA
TGGAATGCTTTGTCCTAAAAGTGTTAGAAATCAATTAAAAAAAGTTAAGG
AAAGTTCTGAAAAAGATGACAAAAAAGTTGATAGTAAAGATGGGATTAAA
ATAAAAACCAATGAAAATTCAAATTCAGTAATTGTAAGTAAGAAGCCGAT
TTTAAATTTATCAGATGGGAAGATATTAGAGATTAAAATAAATAAGGACA
AAATTAATTTTTCCAATGAACTTAGTAAATCTATTAAATCTGAGCCAAAT
CTAAACTCTATTAATGCTATTGTGACTACATCAAATCATAAAACTGACGA
ATCAGATCAATCAATCAAACGAAAAAAGAAAAAGAAACACGGCAAATCTA
AAAATGCAATTGACTCTGACGACGGAAATAAAGATGGAAAAAATAAAAAA
TCTCGGAAATCTAAAAAGAAAAAACATAAGGACATCAGTCCTGAGAAATT
ATCGATTAAGGACGAACTTCATGATGTTAGTGTGGCTAAAGAAACTCCAA
CTGCAGATGTTAAAATAAATAAAGCCCCTATGTCGTTATTGGTGTCATAT
AGTAGCGATGAAGAAGAATCACACAATGAACCGCAAACTAAAGTTATAGA
TATAAAAATATCACCTGAAATTAAAACTGAAGCCATTATCAAAGAACATG
CTGATAATAAAATCGAAATTAAAATAAAACCTACTAAAAGTCCTGGTAAA
TCGGCATGGGATTCATCGGATAGCGAATTGGAAAATGTTGCTAAAGAAAC
AAATATTCCATTTGTTAAATCAAGAAGTCCGTCTGCCGCCATATCGAAGT
GTAGTAAAAAGTCAAAATCTTATAGTAAAGAAAAATCAGAATCAAAATCA
AAATCGAAGTCAAAATCGAAATCTTTTTCAAAATCACATAGCGGAAGTCG
AAGTAAATCAGGTAGCCATTCTCGAAGTAAAACAAACAGTAGGTCAAGTA
AATCTCGTTCCGGTTCAAGTGACTCACGAAGTCGGTCATATAGCAGGTCT
CATAGTAAATCATACTCAGAATCATCAGATAGTCGATCTAGATCATATAC
TCGTTCGAGATCGCGATCAAAAAGGAAATCAAGACGCCATAGGCGACATT
CTTCTTCTTACAGTCGTTCACGCTCGAGATATCGTAGTCGTTCAGTGTCA
AGATCAAGTAGTGACAGTGGATCTAGTCGTTCTTACAGTCGATCTCGTTC
AAGATTTAAGTCAAGTTATTCCACCCGATATCGATCCCGATCACGTTCTA
GATCTTCTCGATCTCGATCTCGAAATTCTTATTACAGAAGCTCATATCGA
AGACACAAAAGAAGTTCATCGCCGGATAATTTTCTCAATCCACAAAATAG
AAAAACCAAACAAATACTTAATCGCAATTCACTCCTAGGTGGAGTTCCCA
AACCTATTGATATCAGTAAAAAAATCGATGCAATCAAAGAATCTCAGAAA
AAAGCTGCTGAAGCCCACAGTTTATCAAATTTAAATATTCCATTACCACC
TGAAAAACCCAAAACTACTCCAATGAAACCCGGCCCGGGTGAATGGTGTC
CATCAATGGATTTTGGTGGCCCCGTTGCTAAAAAGGCCAAGAAAACAGCT
GGACAAAAAATGACCGTGGAAAACAAGCCCGGAGCAGAATCGAATGAGAT
GAATTCCGGATCAGAAAATCATCTAGTAGATTATAACTATATCGGGCCTC
AAGTATCAAGTGATATGGCCAAAAAGCTAGGTTTAGATGTAAAAGCAACG
AAAAAACAAGAAAAAGAAAACTCCAAAAAGGGAGGCACTGAGCACACTCT
CGGTGATTCGTATTTAACTGCAGCTCTCGACGCGAATCACTTGGCATTGC
AATCCCGAGCCTATCAGCATCGGCTAAATAATCTCCAAGGACATAATAAC
TCTGATTTTGGAGGACAAACTGCACAATATTTCCAATCTCTTTACATGTC
CCCGTCATCATTGACCAATAATTTGACTGCTACTGCACTCGCTGCGGTTG
CCAGTGAGCAATTACCGGCTTCGGTGGCAGGCAGTTTAGCTCAGGCTGCT
GTGCAAAGAATCATGTCGTCGTCTAACTTAACTGCTACTTACAATACTCC
GTCTGTGGTTGCGACTCCAACAGCAAGTTATCAGTCAGCCTTACAGCATA
CTTTACCTGGATCATGTAGTACACTCGTAGCAAGCACCCCATCTGGACTG
GTTCCCATTTCGCTTTTACAGCAGGGAGGCGTATCATATGGAATAAACCA
GCTAGTTTTACAACATCTCCAGCAACAGGCATTAAGTGCCCAATTAGGAC
AAGCTATTACAATCCCTAACCAATCAATTTTAGGAGGTTTAAATCCGCTT
CTTATCCAGCAGGCGCAAAATCAACAGGCCCTGTTACAGTCATTTGGGTT
TGGAGCCCAACCCCAACTATTAGCATCTCCGTTAAATTTTCAACACACAC
TGTTAATGGCAGCTCAGCAGCAACAACAGCAGATGATTGCTGCCGCTCAA
TTAAATCAAGCAGCAACGATTCAAGCCGCACAATTGCAAGCTCAAATACA
GGCACAAGCTAGGGCTGCGTCTCTTCAGAATCTTCAACAGGCTGCTCAAA
TGCAATCATCCGCTCAAAATGCTGCAGCAGTAGCTATGGCGGCCTCTGTT
GCTATGGATCAATTTTCGGAACAGTTGGCTAAAGCCGAAGCTGAACAACA
AAATCAAAGTAGTCAAGTTCAAATTTCAGCTGGTGATGAATCCAAGGCAT
TAAATATAGGAGACTATCAGATGGTGTCGGAACAACAGCAAATGGCGTCT
TCTCTTCATTCGATCAACAACGAGTCCACTACCAATTTAATAACGTCCTC
AACTACAGAACTCAACCAATTCGAAGAATCAGCTGTCAATACAACGGTGA
CGTCAGAGCCGTCTTCAGAAACAATACTATTCAATAGCGAATCAGAAATC
GAGTCCATATCCAATGATATCAGTAGTAGTAGTTCCAATGGTGTGATTAA
GCAAGCTCCTAGAATCATATATAATTCTCCCGCTGTGATGGCATCATTAG
TTACAACTCCAATTACTAAACCTTGATATTTTGCATTTTTTGTCTGATGA
TTTCTTTAATGATAACTATTTGTTTATATATGGTTTGTTTAATATCAATT
GTTTTCATTTGGATTATTTTCAGAAATACAATTATAAATCTCTCTAATTT
ATCGGTTGCGTTAATACATTGTACATTCTCTGAGTCTCTGTGGTGTGGTT
AATGTTTTTTTTGGTTAGGCCAAATTTCAAGCTTATTTTGTATTTATACG
ACAATGCAATTTTTATTAAAGTTTCACGATGTAAAATAGAAGTTTGAATC
ATTGGTAACATTTCTTCGTTATGTTTTACTAGTGTGTATTATTCCTGAAA
AAACGAG
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protein sequence of SMED30012483-orf-1

>SMED30012483-orf-1 ID=SMED30012483-orf-1|Name=SMED30012483-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=218bp
MLLKKQIFHLLNQEVRLPPYRSVVKSQNLIVKKNQNQNQNRSQNRNLFQN
HIAEVEVNQVAILEVKQTVGQVNLVPVQVTHEVGHIAGLIVNHTQNHQIV
DLDHILVRDRDQKGNQDAIGDILLLTVVHARDIVVVQCQDQVVTVDLVVL
TVDLVQDLSQVIPPDIDPDHVLDLLDLDLEILITEAHIEDTKEVHRRIIF
SIHKIEKPNKYLIAIHS*
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protein sequence of SMED30012483-orf-2

>SMED30012483-orf-2 ID=SMED30012483-orf-2|Name=SMED30012483-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1071bp
MKKSDDKKPTSEKTKSKSDKGAESKSEIESSEKFKNTSLKPISDKNGMLC
PKSVRNQLKKVKESSEKDDKKVDSKDGIKIKTNENSNSVIVSKKPILNLS
DGKILEIKINKDKINFSNELSKSIKSEPNLNSINAIVTTSNHKTDESDQS
IKRKKKKKHGKSKNAIDSDDGNKDGKNKKSRKSKKKKHKDISPEKLSIKD
ELHDVSVAKETPTADVKINKAPMSLLVSYSSDEEESHNEPQTKVIDIKIS
PEIKTEAIIKEHADNKIEIKIKPTKSPGKSAWDSSDSELENVAKETNIPF
VKSRSPSAAISKCSKKSKSYSKEKSESKSKSKSKSKSFSKSHSGSRSKSG
SHSRSKTNSRSSKSRSGSSDSRSRSYSRSHSKSYSESSDSRSRSYTRSRS
RSKRKSRRHRRHSSSYSRSRSRYRSRSVSRSSSDSGSSRSYSRSRSRFKS
SYSTRYRSRSRSRSSRSRSRNSYYRSSYRRHKRSSSPDNFLNPQNRKTKQ
ILNRNSLLGGVPKPIDISKKIDAIKESQKKAAEAHSLSNLNIPLPPEKPK
TTPMKPGPGEWCPSMDFGGPVAKKAKKTAGQKMTVENKPGAESNEMNSGS
ENHLVDYNYIGPQVSSDMAKKLGLDVKATKKQEKENSKKGGTEHTLGDSY
LTAALDANHLALQSRAYQHRLNNLQGHNNSDFGGQTAQYFQSLYMSPSSL
TNNLTATALAAVASEQLPASVAGSLAQAAVQRIMSSSNLTATYNTPSVVA
TPTASYQSALQHTLPGSCSTLVASTPSGLVPISLLQQGGVSYGINQLVLQ
HLQQQALSAQLGQAITIPNQSILGGLNPLLIQQAQNQQALLQSFGFGAQP
QLLASPLNFQHTLLMAAQQQQQQMIAAAQLNQAATIQAAQLQAQIQAQAR
AASLQNLQQAAQMQSSAQNAAAVAMAASVAMDQFSEQLAKAEAEQQNQSS
QVQISAGDESKALNIGDYQMVSEQQQMASSLHSINNESTTNLITSSTTEL
NQFEESAVNTTVTSEPSSETILFNSESEIESISNDISSSSSNGVIKQAPR
IIYNSPAVMASLVTTPITKP*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron