ralBP1-associated Eps domain-containing protein 1 isoform X8

Overview
NameralBP1-associated Eps domain-containing protein 1 isoform X8
Smed IDSMED30012449
Length (bp)2242
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of ralBP1-associated Eps domain-containing protein 1 isoform X8 (SMED30012449) t-SNE clustered cells

Violin plots show distribution of expression levels for ralBP1-associated Eps domain-containing protein 1 isoform X8 (SMED30012449) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of ralBP1-associated Eps domain-containing protein 1 isoform X8 (SMED30012449) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for ralBP1-associated Eps domain-containing protein 1 isoform X8 (SMED30012449) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012449

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30012449h1SMcG0022284 tr5_3835mu_Smed_v1PMID:25558068
Reuter et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Human
Match: REPS1 (RALBP1 associated Eps domain containing 1 [Source:HGNC Symbol;Acc:HGNC:15578])

HSP 1 Score: 77.0258 bits (188), Expect = 3.229e-15
Identity = 41/108 (37.96%), Postives = 60/108 (55.56%), Query Frame = 2 
Query:   74 MTQFNIKLEETLFYEKLFAQCDVQKVGKVS-GLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEE 394
            M    +   E  +Y  LF+ CD++   KV       +LF  + +P D + +I E CGA RLG+FGRSQFYIALK++++AQ+G  +    +       LPRF    NE+
Sbjct:    1 MEGLTLSDAEQKYYSDLFSYCDIESTKKVVVNGRVLELFRAAQLPNDVVLQIMELCGATRLGYFGRSQFYIALKLVAVAQSGFPLRVESINTVKDLPLPRFVASKNEQ 108
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Human
Match: REPS1 (RALBP1 associated Eps domain containing 1 [Source:HGNC Symbol;Acc:HGNC:15578])

HSP 1 Score: 77.0258 bits (188), Expect = 3.229e-15
Identity = 41/108 (37.96%), Postives = 60/108 (55.56%), Query Frame = 2 
Query:   74 MTQFNIKLEETLFYEKLFAQCDVQKVGKVS-GLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEE 394
            M    +   E  +Y  LF+ CD++   KV       +LF  + +P D + +I E CGA RLG+FGRSQFYIALK++++AQ+G  +    +       LPRF    NE+
Sbjct:    1 MEGLTLSDAEQKYYSDLFSYCDIESTKKVVVNGRVLELFRAAQLPNDVVLQIMELCGATRLGYFGRSQFYIALKLVAVAQSGFPLRVESINTVKDLPLPRFVASKNEQ 108
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Human
Match: REPS1 (RALBP1 associated Eps domain containing 1 [Source:HGNC Symbol;Acc:HGNC:15578])

HSP 1 Score: 77.0258 bits (188), Expect = 3.229e-15
Identity = 41/108 (37.96%), Postives = 60/108 (55.56%), Query Frame = 2 
Query:   74 MTQFNIKLEETLFYEKLFAQCDVQKVGKVS-GLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEE 394
            M    +   E  +Y  LF+ CD++   KV       +LF  + +P D + +I E CGA RLG+FGRSQFYIALK++++AQ+G  +    +       LPRF    NE+
Sbjct:    1 MEGLTLSDAEQKYYSDLFSYCDIESTKKVVVNGRVLELFRAAQLPNDVVLQIMELCGATRLGYFGRSQFYIALKLVAVAQSGFPLRVESINTVKDLPLPRFVASKNEQ 108
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Human
Match: REPS1 (RALBP1 associated Eps domain containing 1 [Source:HGNC Symbol;Acc:HGNC:15578])

HSP 1 Score: 78.5666 bits (192), Expect = 2.252e-14
Identity = 41/108 (37.96%), Postives = 60/108 (55.56%), Query Frame = 2 
Query:   74 MTQFNIKLEETLFYEKLFAQCDVQKVGKVS-GLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEE 394
            M    +   E  +Y  LF+ CD++   KV       +LF  + +P D + +I E CGA RLG+FGRSQFYIALK++++AQ+G  +    +       LPRF    NE+
Sbjct:    1 MEGLTLSDAEQKYYSDLFSYCDIESTKKVVVNGRVLELFRAAQLPNDVVLQIMELCGATRLGYFGRSQFYIALKLVAVAQSGFPLRVESINTVKDLPLPRFVASKNEQ 108
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Human
Match: REPS1 (RALBP1 associated Eps domain containing 1 [Source:HGNC Symbol;Acc:HGNC:15578])

HSP 1 Score: 78.5666 bits (192), Expect = 2.265e-14
Identity = 41/108 (37.96%), Postives = 60/108 (55.56%), Query Frame = 2 
Query:   74 MTQFNIKLEETLFYEKLFAQCDVQKVGKVS-GLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEE 394
            M    +   E  +Y  LF+ CD++   KV       +LF  + +P D + +I E CGA RLG+FGRSQFYIALK++++AQ+G  +    +       LPRF    NE+
Sbjct:    1 MEGLTLSDAEQKYYSDLFSYCDIESTKKVVVNGRVLELFRAAQLPNDVVLQIMELCGATRLGYFGRSQFYIALKLVAVAQSGFPLRVESINTVKDLPLPRFVASKNEQ 108
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Fly
Match: Eps-15 (gene:FBgn0035060 transcript:FBtr0072435)

HSP 1 Score: 58.5362 bits (140), Expect = 2.383e-8
Identity = 30/81 (37.04%), Postives = 44/81 (54.32%), Query Frame = 2 
Query:  113 YEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVI 355
            +E++F Q D+ K G VSGLE   +F+KSGIP   L  I   C  N+ G     QF +A+  +   Q G D   ++L  N++
Sbjct:  318 FEEIFRQSDLDKDGLVSGLEVKDIFIKSGIPQRSLADIWALCDTNQSGKLTVEQFALAMWFVERKQRGVD-PPHVLNANMV 397
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Fly
Match: Eps-15 (gene:FBgn0035060 transcript:FBtr0072434)

HSP 1 Score: 58.151 bits (139), Expect = 2.607e-8
Identity = 30/81 (37.04%), Postives = 44/81 (54.32%), Query Frame = 2 
Query:  113 YEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVI 355
            +E++F Q D+ K G VSGLE   +F+KSGIP   L  I   C  N+ G     QF +A+  +   Q G D   ++L  N++
Sbjct:  318 FEEIFRQSDLDKDGLVSGLEVKDIFIKSGIPQRSLADIWALCDTNQSGKLTVEQFALAMWFVERKQRGVD-PPHVLNANMV 397
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Fly
Match: Eps-15 (gene:FBgn0035060 transcript:FBtr0072433)

HSP 1 Score: 57.7658 bits (138), Expect = 3.888e-8
Identity = 30/81 (37.04%), Postives = 44/81 (54.32%), Query Frame = 2 
Query:  113 YEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVI 355
            +E++F Q D+ K G VSGLE   +F+KSGIP   L  I   C  N+ G     QF +A+  +   Q G D   ++L  N++
Sbjct:  318 FEEIFRQSDLDKDGLVSGLEVKDIFIKSGIPQRSLADIWALCDTNQSGKLTVEQFALAMWFVERKQRGVDP-PHVLNANMV 397
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Fly
Match: Eps-15 (gene:FBgn0035060 transcript:FBtr0308684)

HSP 1 Score: 57.3806 bits (137), Expect = 3.972e-8
Identity = 30/81 (37.04%), Postives = 44/81 (54.32%), Query Frame = 2 
Query:  113 YEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVI 355
            +E++F Q D+ K G VSGLE   +F+KSGIP   L  I   C  N+ G     QF +A+  +   Q G D   ++L  N++
Sbjct:  318 FEEIFRQSDLDKDGLVSGLEVKDIFIKSGIPQRSLADIWALCDTNQSGKLTVEQFALAMWFVERKQRGVD-PPHVLNANMV 397
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Fly
Match: Reps (gene:FBgn0032341 transcript:FBtr0306139)

HSP 1 Score: 56.9954 bits (136), Expect = 6.380e-8
Identity = 32/92 (34.78%), Postives = 47/92 (51.09%), Query Frame = 2 
Query:  101 ETLFYEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGF-FGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRF 373
            ET FY +LF  CDV+K GKV  L+A +LF  + I  + + +I    G         R+QFY  LK+I+  QA   + + ++       LP F
Sbjct:    9 ETRFYSELFQCCDVEKTGKVPMLKATELFRSADIANETVIEITGLAGIPSTALHISRTQFYSCLKLIAAHQAAMPLRQELILATFPLPLPHF 100

HSP 2 Score: 51.9878 bits (123), Expect = 2.344e-6
Identity = 30/89 (33.71%), Postives = 43/89 (48.31%), Query Frame = 2 
Query:  944 TPEQLEYYTKCFQTIQTXXXXXXXXXXXXITIFRMHNLQDECLDRILYLISITAESQITLVEFSKALHLITHVKNGFALPETLPENLLP 1210
            TPEQ EYY K F+T+Q          +     F    +  E L  I  L  +T +  ++L EF+ A+HL+   +N   LP +LP  L P
Sbjct:  263 TPEQREYYNKQFRTVQRDPHGLLSGQAAR-NFFEKSRIPVEELRHIWQLCDVTRDGALSLSEFTAAMHLVVLRRNNIPLPTSLPHCLHP 350
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Zebrafish
Match: reps1 (RALBP1 associated Eps domain containing 1 [Source:ZFIN;Acc:ZDB-GENE-030131-9536])

HSP 1 Score: 76.6406 bits (187), Expect = 7.227e-14
Identity = 43/99 (43.43%), Postives = 57/99 (57.58%), Query Frame = 2 
Query:  101 ETLFYEKLFAQCDVQKVGKV-SGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEE 394
            E  +Y +LF  CDV    KV S  +  +LF  + +  + + +I E CGA RLG FGRSQFYIALK+I+LAQ+G  +    L       LPRF    NE+
Sbjct:   10 EQKYYSELFLSCDVDNAKKVASNGKVLELFRAAQLSNEVVLQITELCGATRLGHFGRSQFYIALKLIALAQSGLPLRLESLNTVRDLPLPRFVMGKNEQ 108
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Zebrafish
Match: CABZ01066725.1 (epidermal growth factor receptor pathway substrate 15-like 1b [Source:NCBI gene;Acc:100329948])

HSP 1 Score: 65.4698 bits (158), Expect = 1.451e-10
Identity = 29/87 (33.33%), Postives = 48/87 (55.17%), Query Frame = 2 
Query:  113 YEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRF 373
            YE L+ Q D    G+V   EA +   KSG+    L +I +    +R G+  +  F+ AL++++ AQ G+DV  N L +N+   +P+F
Sbjct:   17 YESLYRQVDPANTGRVGAAEAAQFLKKSGLSDSTLGQIWDLSDPDRKGYLDKKGFFTALRLVASAQGGSDVSLNSLSQNISAPMPKF 103
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Zebrafish
Match: eps15 (epidermal growth factor receptor pathway substrate 15 [Source:ZFIN;Acc:ZDB-GENE-081104-264])

HSP 1 Score: 55.4546 bits (132), Expect = 2.600e-7
Identity = 27/74 (36.49%), Postives = 40/74 (54.05%), Query Frame = 2 
Query:   77 TQFNIKLEETLFYEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKII 298
            +Q+ +   +   Y+ LFA+ D    G VSG E   +FLK+G+P   L +I E C    +G   R QF +AL +I
Sbjct:  250 SQWVVSPADKAKYDDLFAKTDTDMDGLVSGAEVRDIFLKTGLPSATLARIWELCDIGDVGKLTRDQFALALYLI 323
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Zebrafish
Match: eps15l1a (epidermal growth factor receptor pathway substrate 15-like 1a [Source:ZFIN;Acc:ZDB-GENE-060503-274])

HSP 1 Score: 55.4546 bits (132), Expect = 2.616e-7
Identity = 29/87 (33.33%), Postives = 45/87 (51.72%), Query Frame = 2 
Query:  113 YEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRF 373
            YE  + Q D    G+V   EA     KSG+P   L KI +    +  GF  +  FY+AL++++ AQ+G D+  +I   N+    P+F
Sbjct:   17 YENFYRQVDPGNTGRVGPTEAALFLKKSGLPDITLGKIWDLADPDGKGFLDKQGFYVALRLVACAQSGHDI--SISSLNLPVPPPKF 101
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Zebrafish
Match: eps15 (epidermal growth factor receptor pathway substrate 15 [Source:ZFIN;Acc:ZDB-GENE-081104-264])

HSP 1 Score: 55.4546 bits (132), Expect = 2.678e-7
Identity = 27/74 (36.49%), Postives = 40/74 (54.05%), Query Frame = 2 
Query:   77 TQFNIKLEETLFYEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKII 298
            +Q+ +   +   Y+ LFA+ D    G VSG E   +FLK+G+P   L +I E C    +G   R QF +AL +I
Sbjct:  250 SQWVVSPADKAKYDDLFAKTDTDMDGLVSGAEVRDIFLKTGLPSATLARIWELCDIGDVGKLTRDQFALALYLI 323
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Xenopus
Match: REPS2 (RALBP1 associated Eps domain containing 2 [Source:NCBI gene;Acc:100497375])

HSP 1 Score: 66.2402 bits (160), Expect = 1.414e-10
Identity = 28/73 (38.36%), Postives = 46/73 (63.01%), Query Frame = 2 
Query:  113 YEKLFAQCDVQKVGKVSGL--EAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDV 325
            Y +LF+ C  +   +++    +  +LF  S +P D L +I E CGA  +G+FG +QF++ALK+I++AQ G  V
Sbjct:   42 YSELFSMCQAEGSARLAAGSNKVGELFRASKLPADTLHQITELCGAKHVGYFGPAQFFVALKLIAVAQTGLPV 114

HSP 2 Score: 51.6026 bits (122), Expect = 4.232e-6
Identity = 34/88 (38.64%), Postives = 42/88 (47.73%), Query Frame = 2 
Query:  944 TPEQLEYYTKCFQTIQTXXXXXXXXXXXXITIFRMHNLQDECLDRILYLISITAESQITLVEFSKALHLITHVKNGFALPETLPENLL 1207
            T EQ EYYT  F T+Q    S    +      F    L    L  I  L  +  +  +TL EFS A HLI   KNG+ +P+TLP  LL
Sbjct:  265 TEEQREYYTNQFITLQPDLKSLISGSVAK-NFFTKSKLPISELSHIWDLSDVDCDGALTLTEFSAAFHLIVARKNGYEVPDTLPRTLL 351
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Mouse
Match: Reps1 (RalBP1 associated Eps domain containing protein [Source:MGI Symbol;Acc:MGI:1196373])

HSP 1 Score: 76.6406 bits (187), Expect = 2.508e-15
Identity = 41/108 (37.96%), Postives = 60/108 (55.56%), Query Frame = 2 
Query:   74 MTQFNIKLEETLFYEKLFAQCDVQKVGKVS-GLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEE 394
            M    +   E  +Y  LF+ CD++   KV       +LF  + +P D + +I E CGA RLG+FGRSQFYIALK++++AQ+G  +    +       LPRF    NE+
Sbjct:    1 MEGLTLSDAEQKYYSDLFSYCDIESTKKVVVNGRVLELFRAAQLPNDVVLQIMELCGATRLGYFGRSQFYIALKLVAVAQSGFPLRVESINTVKDLPLPRFVASKNEQ 108
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Mouse
Match: Reps1 (RalBP1 associated Eps domain containing protein [Source:MGI Symbol;Acc:MGI:1196373])

HSP 1 Score: 72.0182 bits (175), Expect = 5.765e-15
Identity = 34/73 (46.58%), Postives = 49/73 (67.12%), Query Frame = 2 
Query:  101 ETLFYEKLFAQCDVQKVGKVS-GLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAG 316
            E  +Y  LF+ CD++   KV       +LF  + +P D + +I E CGA RLG+FGRSQFYIALK++++AQ+G
Sbjct:   10 EQKYYSDLFSYCDIESTKKVVVNGRVLELFRAAQLPNDVVLQIMELCGATRLGYFGRSQFYIALKLVAVAQSG 82
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Mouse
Match: Reps1 (RalBP1 associated Eps domain containing protein [Source:MGI Symbol;Acc:MGI:1196373])

HSP 1 Score: 78.5666 bits (192), Expect = 1.754e-14
Identity = 41/108 (37.96%), Postives = 60/108 (55.56%), Query Frame = 2 
Query:   74 MTQFNIKLEETLFYEKLFAQCDVQKVGKVS-GLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEE 394
            M    +   E  +Y  LF+ CD++   KV       +LF  + +P D + +I E CGA RLG+FGRSQFYIALK++++AQ+G  +    +       LPRF    NE+
Sbjct:    1 MEGLTLSDAEQKYYSDLFSYCDIESTKKVVVNGRVLELFRAAQLPNDVVLQIMELCGATRLGYFGRSQFYIALKLVAVAQSGFPLRVESINTVKDLPLPRFVASKNEQ 108
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Mouse
Match: Reps1 (RalBP1 associated Eps domain containing protein [Source:MGI Symbol;Acc:MGI:1196373])

HSP 1 Score: 78.5666 bits (192), Expect = 1.866e-14
Identity = 41/108 (37.96%), Postives = 60/108 (55.56%), Query Frame = 2 
Query:   74 MTQFNIKLEETLFYEKLFAQCDVQKVGKVS-GLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEE 394
            M    +   E  +Y  LF+ CD++   KV       +LF  + +P D + +I E CGA RLG+FGRSQFYIALK++++AQ+G  +    +       LPRF    NE+
Sbjct:    1 MEGLTLSDAEQKYYSDLFSYCDIESTKKVVVNGRVLELFRAAQLPNDVVLQIMELCGATRLGYFGRSQFYIALKLVAVAQSGFPLRVESINTVKDLPLPRFVASKNEQ 108
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Mouse
Match: Reps1 (RalBP1 associated Eps domain containing protein [Source:MGI Symbol;Acc:MGI:1196373])

HSP 1 Score: 78.1814 bits (191), Expect = 2.059e-14
Identity = 41/108 (37.96%), Postives = 60/108 (55.56%), Query Frame = 2 
Query:   74 MTQFNIKLEETLFYEKLFAQCDVQKVGKVS-GLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEE 394
            M    +   E  +Y  LF+ CD++   KV       +LF  + +P D + +I E CGA RLG+FGRSQFYIALK++++AQ+G  +    +       LPRF    NE+
Sbjct:    1 MEGLTLSDAEQKYYSDLFSYCDIESTKKVVVNGRVLELFRAAQLPNDVVLQIMELCGATRLGYFGRSQFYIALKLVAVAQSGFPLRVESINTVKDLPLPRFVASKNEQ 108
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. UniProt/SwissProt
Match: sp|Q96D71|REPS1_HUMAN (RalBP1-associated Eps domain-containing protein 1 OS=Homo sapiens OX=9606 GN=REPS1 PE=1 SV=3)

HSP 1 Score: 77.7962 bits (190), Expect = 2.075e-13
Identity = 41/108 (37.96%), Postives = 60/108 (55.56%), Query Frame = 2 
Query:   74 MTQFNIKLEETLFYEKLFAQCDVQKVGKVS-GLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEE 394
            M    +   E  +Y  LF+ CD++   KV       +LF  + +P D + +I E CGA RLG+FGRSQFYIALK++++AQ+G  +    +       LPRF    NE+
Sbjct:    1 MEGLTLSDAEQKYYSDLFSYCDIESTKKVVVNGRVLELFRAAQLPNDVVLQIMELCGATRLGYFGRSQFYIALKLVAVAQSGFPLRVESINTVKDLPLPRFVASKNEQ 108
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. UniProt/SwissProt
Match: sp|O54916|REPS1_MOUSE (RalBP1-associated Eps domain-containing protein 1 OS=Mus musculus OX=10090 GN=Reps1 PE=1 SV=2)

HSP 1 Score: 77.7962 bits (190), Expect = 2.441e-13
Identity = 41/108 (37.96%), Postives = 60/108 (55.56%), Query Frame = 2 
Query:   74 MTQFNIKLEETLFYEKLFAQCDVQKVGKVS-GLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEE 394
            M    +   E  +Y  LF+ CD++   KV       +LF  + +P D + +I E CGA RLG+FGRSQFYIALK++++AQ+G  +    +       LPRF    NE+
Sbjct:    1 MEGLTLSDAEQKYYSDLFSYCDIESTKKVVVNGRVLELFRAAQLPNDVVLQIMELCGATRLGYFGRSQFYIALKLVAVAQSGFPLRVESINTVKDLPLPRFVASKNEQ 108
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. UniProt/SwissProt
Match: sp|Q8NFH8|REPS2_HUMAN (RalBP1-associated Eps domain-containing protein 2 OS=Homo sapiens OX=9606 GN=REPS2 PE=1 SV=2)

HSP 1 Score: 63.1586 bits (152), Expect = 6.029e-9
Identity = 30/64 (46.88%), Postives = 42/64 (65.62%), Query Frame = 2 
Query:  182 LFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRF 373
            LF  S +P + L +I E CGA R+G+FG +QFYIALK+I+ AQ+G  V    ++  +   LPRF
Sbjct:   78 LFRASQLPAETLHQITELCGAKRVGYFGPTQFYIALKLIAAAQSGLPVRIESIKCEL--PLPRF 139
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. UniProt/SwissProt
Match: sp|B3LF48|EHD2_ARATH (EH domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=EHD2 PE=1 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 6.698e-7
Identity = 28/85 (32.94%), Postives = 48/85 (56.47%), Query Frame = 2 
Query:   98 EETLFYEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNV 352
            E    Y++ F   D    G+VSG +A K F  S +   +LK++     + R GF G S+F  A+K++SLAQ G ++  ++L+ ++
Sbjct:   15 EHQKIYKEWFNIADSDGDGRVSGNDATKFFAMSKLSRQELKQVWAVADSKRQGFLGLSEFITAMKLVSLAQEGHEITSDLLKGSI 99
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. TrEMBL
Match: A0A0B6XXR3 (EH domain-containing protein (Fragment) OS=Arion vulgaris OX=1028688 GN=ORF3669 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.452e-23
Identity = 52/109 (47.71%), Postives = 71/109 (65.14%), Query Frame = 2 
Query:   74 MTQFNIKLEETLFYEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEEAF 400
            M    + + E  +Y +LF  CD++  GKV+GL+A +LFL SG+  D LK+I E CGA RLG FGRSQFY+ALK+++LAQAG  V  + L       LP+F  + NE+ F
Sbjct:    7 MEGLKLGVREQQYYAELFQSCDIEGSGKVTGLKASELFLTSGLSPDILKQITELCGAKRLGHFGRSQFYVALKLVALAQAGLPVRLDALNSGKDFPLPKFSSKGNEQVF 115
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. TrEMBL
Match: A0A0B6ZHX0 (Uncharacterized protein (Fragment) OS=Arion vulgaris OX=1028688 GN=ORF65247 PE=4 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 3.002e-20
Identity = 50/109 (45.87%), Postives = 68/109 (62.39%), Query Frame = 2 
Query:   74 MTQFNIKLEETLFYEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEEAF 400
            M +  + + E  +Y +LF  CD +  GK+SG +A +LFL SG+  D LK+I E CGA RLG FGRSQFYIALK ++LAQAG  +  + L       LP+F  +  E+ F
Sbjct:    1 MERLKLGVREQQYYAELFQSCDTEGSGKISGPQASELFLSSGLSPDVLKQITELCGAKRLGHFGRSQFYIALKFVALAQAGIPIKMDALNSGKDIPLPKFSLKGTEQGF 109
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. TrEMBL
Match: A0A0B6ZHP9 (Uncharacterized protein OS=Arion vulgaris OX=1028688 GN=ORF65251 PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 4.298e-20
Identity = 50/109 (45.87%), Postives = 68/109 (62.39%), Query Frame = 2 
Query:   74 MTQFNIKLEETLFYEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEEAF 400
            M +  + + E  +Y +LF  CD +  GK+SG +A +LFL SG+  D LK+I E CGA RLG FGRSQFYIALK ++LAQAG  +  + L       LP+F  +  E+ F
Sbjct:    1 MERLKLGVREQQYYAELFQSCDTEGSGKISGPQASELFLSSGLSPDVLKQITELCGAKRLGHFGRSQFYIALKFVALAQAGIPIKMDALNSGKDIPLPKFSLKGTEQGF 109
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. TrEMBL
Match: A0A2T7P548 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_11135 PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 6.817e-17
Identity = 46/99 (46.46%), Postives = 62/99 (62.63%), Query Frame = 2 
Query:  101 ETLFYEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEEA 397
            E  +Y +LF  CDV+  G+++G +A +LFL S +  D LK+I E CGA RLG  GRSQFYIALK I++AQAG  +    L   V   LPRF  +  ++ 
Sbjct:   10 EQQYYAELFQTCDVEGTGRITGPKASELFLASSLNQDTLKQIWELCGAKRLGHLGRSQFYIALKFIAVAQAGLPLKLETLNAGVDIPLPRFSSKSTDQG 108
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. TrEMBL
Match: A0A210PTA5 (RalBP1-associated Eps domain-containing protein 1 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT07912 PE=4 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 8.907e-17
Identity = 41/84 (48.81%), Postives = 54/84 (64.29%), Query Frame = 2 
Query:   74 MTQFNIKLEETLFYEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDV 325
            M    +  +E + Y +LF  CDV   G+++G+ A  LFL SG+  D L ++ E CGA RLG FGRSQFYIALK+I+ AQ G  V
Sbjct:    3 MEGLTLSQQEQICYGELFQTCDVDGTGRITGVRASDLFLSSGLSQDHLMQVTELCGAKRLGHFGRSQFYIALKLIAAAQLGLPV 86
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Cavefish
Match: reps1 (RALBP1 associated Eps domain containing 1 [Source:NCBI gene;Acc:103026641])

HSP 1 Score: 75.0998 bits (183), Expect = 1.752e-13
Identity = 43/99 (43.43%), Postives = 55/99 (55.56%), Query Frame = 2 
Query:  101 ETLFYEKLFAQCDVQKVGKV-SGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEE 394
            E  +Y  LF  CDV    KV S  +   LF  + +  D + +I E CGA RLG FGRSQFYIALK+I++AQ+G  +    L       LPRF    NE+
Sbjct:   10 EQKYYSDLFIYCDVDNTKKVASNGKVLDLFRAAQLSNDVVLQITELCGATRLGHFGRSQFYIALKLIAIAQSGLPLRVESLNSVKDLPLPRFVVGKNEQ 108
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Cavefish
Match: reps1 (RALBP1 associated Eps domain containing 1 [Source:NCBI gene;Acc:103026641])

HSP 1 Score: 74.7146 bits (182), Expect = 1.845e-13
Identity = 43/99 (43.43%), Postives = 55/99 (55.56%), Query Frame = 2 
Query:  101 ETLFYEKLFAQCDVQKVGKV-SGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEE 394
            E  +Y  LF  CDV    KV S  +   LF  + +  D + +I E CGA RLG FGRSQFYIALK+I++AQ+G  +    L       LPRF    NE+
Sbjct:   10 EQKYYSDLFIYCDVDNTKKVASNGKVLDLFRAAQLSNDVVLQITELCGATRLGHFGRSQFYIALKLIAIAQSGLPLRVESLNSVKDLPLPRFVVGKNEQ 108
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Cavefish
Match: reps1 (RALBP1 associated Eps domain containing 1 [Source:NCBI gene;Acc:103026641])

HSP 1 Score: 74.7146 bits (182), Expect = 1.916e-13
Identity = 43/99 (43.43%), Postives = 55/99 (55.56%), Query Frame = 2 
Query:  101 ETLFYEKLFAQCDVQKVGKV-SGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEE 394
            E  +Y  LF  CDV    KV S  +   LF  + +  D + +I E CGA RLG FGRSQFYIALK+I++AQ+G  +    L       LPRF    NE+
Sbjct:   10 EQKYYSDLFIYCDVDNTKKVASNGKVLDLFRAAQLSNDVVLQITELCGATRLGHFGRSQFYIALKLIAIAQSGLPLRVESLNSVKDLPLPRFVVGKNEQ 108
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Cavefish
Match: ENSAMXT00000045855.1 (epidermal growth factor receptor substrate 15-like 1 [Source:NCBI gene;Acc:103041115])

HSP 1 Score: 63.929 bits (154), Expect = 5.275e-10
Identity = 30/87 (34.48%), Postives = 46/87 (52.87%), Query Frame = 2 
Query:  113 YEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRF 373
            YE  + Q D    G+V   EA +   KSG+    L KI +     R GF  +  F+IAL++++ AQ+G DV ++ L + V    P+F
Sbjct:   17 YESFYRQVDPANTGRVGAAEAAQFLKKSGLSDSTLGKIWDLSDPERKGFLDKRGFFIALRLVASAQSGNDVSQHSLSQTVSIPAPKF 103
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Cavefish
Match: ENSAMXT00000058079.1 (epidermal growth factor receptor substrate 15-like 1 [Source:NCBI gene;Acc:103041115])

HSP 1 Score: 63.929 bits (154), Expect = 5.275e-10
Identity = 30/87 (34.48%), Postives = 46/87 (52.87%), Query Frame = 2 
Query:  113 YEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRF 373
            YE  + Q D    G+V   EA +   KSG+    L KI +     R GF  +  F+IAL++++ AQ+G DV ++ L + V    P+F
Sbjct:   17 YESFYRQVDPANTGRVGAAEAAQFLKKSGLSDSTLGKIWDLSDPERKGFLDKRGFFIALRLVASAQSGNDVSQHSLSQTVSIPAPKF 103
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004914.1 (pep scaffold:Pmarinus_7.0:GL478868:2980:27082:-1 gene:ENSPMAG00000004442.1 transcript:ENSPMAT00000004914.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 68.5514 bits (166), Expect = 3.708e-12
Identity = 31/58 (53.45%), Postives = 40/58 (68.97%), Query Frame = 2 
Query:  200 IPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRF 373
            +P   L +I E CGA RLG+FGRSQFYIALK+I++AQAG  +    L  + +  LPRF
Sbjct:    1 LPPSTLPQITEMCGAKRLGYFGRSQFYIALKLIAVAQAGLPMRLESL--STVRDLPRF 56

HSP 2 Score: 48.521 bits (114), Expect = 4.782e-6
Identity = 32/89 (35.96%), Postives = 41/89 (46.07%), Query Frame = 2 
Query:  944 TPEQLEYYTKCFQTIQTXXXXXXXXXXXXITIFRMHNLQDECLDRILYLISITAESQITLVEFSKALHLITHVKNGFALPETLPENLLP 1210
            T EQ +YY   F+TIQ         +      F    L    L  I  L     +  +TL EF  A HL+   KNG+ LPE LPE+L+P
Sbjct:  249 TEEQRDYYINQFKTIQPDLGGLIPGSGAK-EFFTKSKLPIVELSHIWELSDFDQDGALTLDEFCAAFHLVVARKNGYELPEKLPESLVP 336
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Medaka
Match: reps1 (RALBP1 associated Eps domain containing 1 [Source:NCBI gene;Acc:101156935])

HSP 1 Score: 77.411 bits (189), Expect = 3.320e-14
Identity = 41/92 (44.57%), Postives = 52/92 (56.52%), Query Frame = 2 
Query:  101 ETLFYEKLFAQCDVQKVGKV-SGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRF 373
            E  +Y +LF  CD     KV S      LF  + +P + + +I E CGA RLG FGRSQFYIALK+I++AQAG  +    L       LPRF
Sbjct:   10 EQKYYSELFVYCDTDNTKKVASNGRVLDLFRAAQLPSEVVLQITELCGATRLGHFGRSQFYIALKLIAIAQAGLPLRVESLNSVKDLPLPRF 101
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Medaka
Match: reps1 (RALBP1 associated Eps domain containing 1 [Source:NCBI gene;Acc:101156935])

HSP 1 Score: 77.0258 bits (188), Expect = 3.947e-14
Identity = 43/98 (43.88%), Postives = 54/98 (55.10%), Query Frame = 2 
Query:  101 ETLFYEKLFAQCDVQKVGKV-SGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNE 391
            E  +Y +LF  CD     KV S      LF  + +P + + +I E CGA RLG FGRSQFYIALK+I++AQAG  +    L       LPRF    NE
Sbjct:   10 EQKYYSELFVYCDTDNTKKVASNGRVLDLFRAAQLPSEVVLQITELCGATRLGHFGRSQFYIALKLIAIAQAGLPLRVESLNSVKDLPLPRFIVGKNE 107
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Medaka
Match: reps1 (RALBP1 associated Eps domain containing 1 [Source:NCBI gene;Acc:101156935])

HSP 1 Score: 77.0258 bits (188), Expect = 4.062e-14
Identity = 44/107 (41.12%), Postives = 56/107 (52.34%), Query Frame = 2 
Query:   74 MTQFNIKLEETLFYEKLFAQCDVQKVGKV-SGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNE 391
            M    +   E  +Y +LF  CD     KV S      LF  + +P + + +I E CGA RLG FGRSQFYIALK+I++AQAG  +    L       LPRF    NE
Sbjct:    1 MESLTLSDVEQKYYSELFVYCDTDNTKKVASNGRVLDLFRAAQLPSEVVLQITELCGATRLGHFGRSQFYIALKLIAIAQAGLPLRVESLNSVKDLPLPRFIVGKNE 107
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Medaka
Match: reps2 (RALBP1 associated Eps domain containing 2 [Source:NCBI gene;Acc:101171071])

HSP 1 Score: 74.7146 bits (182), Expect = 1.837e-13
Identity = 34/91 (37.36%), Postives = 52/91 (57.14%), Query Frame = 2 
Query:  101 ETLFYEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRF 373
            E +++  L   C     GK+S  +  +L   S +P + L K+ E CGA  LG+FG +QFY+ALK+++ AQ+G  V    +  N+   LPRF
Sbjct:   24 EQMYFSGLHGLCQADASGKLSSSKVAELLKASQLPAESLHKVTEVCGAKHLGYFGTAQFYVALKLLAAAQSGLPVRLESVTANL--PLPRF 112

HSP 2 Score: 54.299 bits (129), Expect = 4.540e-7
Identity = 44/117 (37.61%), Postives = 54/117 (46.15%), Query Frame = 2 
Query:  914 TDSDDRIPMA-TPEQLEYYTKCFQTIQTXXXXXXXXXXXXITIFRMHNLQDECLDRILYLISITAESQITLVEFSKALHLITHVKNGFALPETLPENLLP----VEFEQPDEPFSFD 1249
            TD  D  P   T EQLEYYT  F+T+Q   D+  +        F    L    L  I  L  +  +  +T  EF  A HLI   KNG+ LPE+LP  L P     E E PD P S +
Sbjct:  250 TDECDNDPWRITEEQLEYYTNQFKTLQPDLDALILGGVAK-NFFTKSKLPIPELSHIWELSDVNKDGALTFHEFCIAFHLIVARKNGYPLPESLPPTLQPGFLLCEEEIPDTPGSAE 365
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Medaka
Match: reps2 (RALBP1 associated Eps domain containing 2 [Source:NCBI gene;Acc:101171071])

HSP 1 Score: 74.3294 bits (181), Expect = 2.119e-13
Identity = 34/91 (37.36%), Postives = 52/91 (57.14%), Query Frame = 2 
Query:  101 ETLFYEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRF 373
            E +++  L   C     GK+S  +  +L   S +P + L K+ E CGA  LG+FG +QFY+ALK+++ AQ+G  V    +  N+   LPRF
Sbjct:   24 EQMYFSGLHGLCQADASGKLSSSKVAELLKASQLPAESLHKVTEVCGAKHLGYFGTAQFYVALKLLAAAQSGLPVRLESVTANL--PLPRF 112

HSP 2 Score: 54.299 bits (129), Expect = 4.222e-7
Identity = 44/117 (37.61%), Postives = 54/117 (46.15%), Query Frame = 2 
Query:  914 TDSDDRIPMA-TPEQLEYYTKCFQTIQTXXXXXXXXXXXXITIFRMHNLQDECLDRILYLISITAESQITLVEFSKALHLITHVKNGFALPETLPENLLP----VEFEQPDEPFSFD 1249
            TD  D  P   T EQLEYYT  F+T+Q   D+  +        F    L    L  I  L  +  +  +T  EF  A HLI   KNG+ LPE+LP  L P     E E PD P S +
Sbjct:  250 TDECDNDPWRITEEQLEYYTNQFKTLQPDLDALILGGVAK-NFFTKSKLPIPELSHIWELSDVNKDGALTFHEFCIAFHLIVARKNGYPLPESLPPTLQPGFLLCEEEIPDTPGSAE 365
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Planmine SMEST
Match: SMESG000023078.1 (SMESG000023078.1)

HSP 1 Score: 1021.54 bits (2640), Expect = 0.000e+0
Identity = 621/622 (99.84%), Postives = 622/622 (100.00%), Query Frame = 2 
Query:   74 MTQFNIKLEETLFYEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEEAFKTVQKTSELPLTILHTNNKVHMRTLSGNNILHKIPPTNRYSYSSPISKTMSQHVSKGSLDNFPMKLDNLNTQXXXXXXXXXXXXVWDGYSEEDNKGLLGPDVKTDFNLFCQRNQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVQDTNPTGYTLMEAVEGLATVPGTTTDSDDRIPMATPEQLEYYTKCFQTIQTXXXXXXXXXXXXITIFRMHNLQDECLDRILYLISITAESQITLVEFSKALHLITHVKNGFALPETLPENLLPVEFEQPDEPFSFDLSDGELPDVSNTYQTXXXXXXXXXXXXXXGFDFTDVAANRNWVQSKSRFLXXXXXXXXXXXXXXXXXXDKKHSLASNNEEAADVSRPRPCAKKXXXXXXXXXXXXXXXXXXXXXXXXENLNKKLSFERNTDPSGDSLSAASCAIYPRESDVQIRKFSETSKSEITNNARFSINQEPRKQLQISMRDLKSKNHVLLRLNNEMTSELEELREQRVNLHLQLEYIKQSQIDIQ 1939
            MTQFNIKLEETLFYEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEEAFKTVQKTSELPLTILHTNNKVHMRTLSGNNILHKIPPTNRYSYSSPISKTMSQHVSKGSLDNFP+KLDNLNTQENNEDEPDEEDNVWDGYSEEDNKGLLGPDVKTDFNLFCQRNQDGDSNEYSSSSDHDSSSFSYESDEDENAENADVQDTNPTGYTLMEAVEGLATVPGTTTDSDDRIPMATPEQLEYYTKCFQTIQTSSDSDQVSASDAITIFRMHNLQDECLDRILYLISITAESQITLVEFSKALHLITHVKNGFALPETLPENLLPVEFEQPDEPFSFDLSDGELPDVSNTYQTSSVESISSVDDERSGFDFTDVAANRNWVQSKSRFLNKNQYSKSSNSFSQSKTSDKKHSLASNNEEAADVSRPRPCAKKSLSLSQPISSSVAPPPPPRSSVPSENLNKKLSFERNTDPSGDSLSAASCAIYPRESDVQIRKFSETSKSEITNNARFSINQEPRKQLQISMRDLKSKNHVLLRLNNEMTSELEELREQRVNLHLQLEYIKQSQIDIQ
Sbjct:    1 MTQFNIKLEETLFYEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRFDQEVNEEAFKTVQKTSELPLTILHTNNKVHMRTLSGNNILHKIPPTNRYSYSSPISKTMSQHVSKGSLDNFPVKLDNLNTQENNEDEPDEEDNVWDGYSEEDNKGLLGPDVKTDFNLFCQRNQDGDSNEYSSSSDHDSSSFSYESDEDENAENADVQDTNPTGYTLMEAVEGLATVPGTTTDSDDRIPMATPEQLEYYTKCFQTIQTSSDSDQVSASDAITIFRMHNLQDECLDRILYLISITAESQITLVEFSKALHLITHVKNGFALPETLPENLLPVEFEQPDEPFSFDLSDGELPDVSNTYQTSSVESISSVDDERSGFDFTDVAANRNWVQSKSRFLNKNQYSKSSNSFSQSKTSDKKHSLASNNEEAADVSRPRPCAKKSLSLSQPISSSVAPPPPPRSSVPSENLNKKLSFERNTDPSGDSLSAASCAIYPRESDVQIRKFSETSKSEITNNARFSINQEPRKQLQISMRDLKSKNHVLLRLNNEMTSELEELREQRVNLHLQLEYIKQSQIDIQ 622
[Close]
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Planmine SMEST
Match: SMESG000037239.1 (SMESG000037239.1)

HSP 1 Score: 53.5286 bits (127), Expect = 5.065e-7
Identity = 27/71 (38.03%), Postives = 41/71 (57.75%), Query Frame = 2 
Query:  113 YEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKKICEYCGANRLGFFGRSQFYIALKIIS--LAQAGT 319
            Y+ LF   D  K G VSGLE    F++SG+P  +L KI + C  N+ G     QF +A+ +I+  ++ AG+
Sbjct:  263 YDNLFFAADKDKDGFVSGLEIKDTFVQSGLPQTELAKIWDLCDINKTGLLNSEQFALAMYLINQQISSAGS 333
The following BLAST results are available for this feature:
BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
REPS13.229e-1537.96RALBP1 associated Eps domain containing 1 [Source:... [more]
REPS13.229e-1537.96RALBP1 associated Eps domain containing 1 [Source:... [more]
REPS13.229e-1537.96RALBP1 associated Eps domain containing 1 [Source:... [more]
REPS12.252e-1437.96RALBP1 associated Eps domain containing 1 [Source:... [more]
REPS12.265e-1437.96RALBP1 associated Eps domain containing 1 [Source:... [more]
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BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Eps-152.383e-837.04gene:FBgn0035060 transcript:FBtr0072435[more]
Eps-152.607e-837.04gene:FBgn0035060 transcript:FBtr0072434[more]
Eps-153.888e-837.04gene:FBgn0035060 transcript:FBtr0072433[more]
Eps-153.972e-837.04gene:FBgn0035060 transcript:FBtr0308684[more]
Reps6.380e-834.78gene:FBgn0032341 transcript:FBtr0306139[more]
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BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
reps17.227e-1443.43RALBP1 associated Eps domain containing 1 [Source:... [more]
CABZ01066725.11.451e-1033.33epidermal growth factor receptor pathway substrate... [more]
eps152.600e-736.49epidermal growth factor receptor pathway substrate... [more]
eps15l1a2.616e-733.33epidermal growth factor receptor pathway substrate... [more]
eps152.678e-736.49epidermal growth factor receptor pathway substrate... [more]
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BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
REPS21.414e-1038.36RALBP1 associated Eps domain containing 2 [Source:... [more]
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BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Reps12.508e-1537.96RalBP1 associated Eps domain containing protein [S... [more]
Reps15.765e-1546.58RalBP1 associated Eps domain containing protein [S... [more]
Reps11.754e-1437.96RalBP1 associated Eps domain containing protein [S... [more]
Reps11.866e-1437.96RalBP1 associated Eps domain containing protein [S... [more]
Reps12.059e-1437.96RalBP1 associated Eps domain containing protein [S... [more]
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BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q96D71|REPS1_HUMAN2.075e-1337.96RalBP1-associated Eps domain-containing protein 1 ... [more]
sp|O54916|REPS1_MOUSE2.441e-1337.96RalBP1-associated Eps domain-containing protein 1 ... [more]
sp|Q8NFH8|REPS2_HUMAN6.029e-946.88RalBP1-associated Eps domain-containing protein 2 ... [more]
sp|B3LF48|EHD2_ARATH6.698e-732.94EH domain-containing protein 2 OS=Arabidopsis thal... [more]
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BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0B6XXR31.452e-2347.71EH domain-containing protein (Fragment) OS=Arion v... [more]
A0A0B6ZHX03.002e-2045.87Uncharacterized protein (Fragment) OS=Arion vulgar... [more]
A0A0B6ZHP94.298e-2045.87Uncharacterized protein OS=Arion vulgaris OX=10286... [more]
A0A2T7P5486.817e-1746.46Uncharacterized protein OS=Pomacea canaliculata OX... [more]
A0A210PTA58.907e-1748.81RalBP1-associated Eps domain-containing protein 1 ... [more]
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BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
reps11.752e-1343.43RALBP1 associated Eps domain containing 1 [Source:... [more]
reps11.845e-1343.43RALBP1 associated Eps domain containing 1 [Source:... [more]
reps11.916e-1343.43RALBP1 associated Eps domain containing 1 [Source:... [more]
ENSAMXT00000045855.15.275e-1034.48epidermal growth factor receptor substrate 15-like... [more]
ENSAMXT00000058079.15.275e-1034.48epidermal growth factor receptor substrate 15-like... [more]
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BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000004914.13.708e-1253.45pep scaffold:Pmarinus_7.0:GL478868:2980:27082:-1 g... [more]
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BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
reps13.320e-1444.57RALBP1 associated Eps domain containing 1 [Source:... [more]
reps13.947e-1443.88RALBP1 associated Eps domain containing 1 [Source:... [more]
reps14.062e-1441.12RALBP1 associated Eps domain containing 1 [Source:... [more]
reps21.837e-1337.36RALBP1 associated Eps domain containing 2 [Source:... [more]
reps22.119e-1337.36RALBP1 associated Eps domain containing 2 [Source:... [more]
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BLAST of ralBP1-associated Eps domain-containing protein 1 isoform X8 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000023078.10.000e+099.84SMESG000023078.1[more]
SMESG000037239.15.065e-738.03SMESG000037239.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012449 ID=SMED30012449|Name=ralBP1-associated Eps domain-containing protein 1 isoform X8|organism=Schmidtea mediterranea sexual|type=transcript|length=2242bp
ATTAAACTAGCCTATTTATTCAAATTTATTTTAAATATATATTAAATTGA
TAATAATTATTAATTAGATTTGAATGACACAGTTTAACATAAAATTAGAG
GAAACTTTGTTTTATGAAAAATTATTTGCGCAATGTGATGTACAAAAAGT
TGGTAAAGTCTCAGGATTAGAAGCCTATAAGTTGTTTTTAAAATCTGGTA
TACCAGTTGATGATTTAAAAAAAATATGTGAATACTGTGGGGCTAATAGA
TTAGGATTCTTTGGTCGAAGTCAATTTTATATTGCCTTGAAAATAATATC
CTTAGCTCAAGCGGGAACTGACGTTGATGAGAATATATTACAAAAAAATG
TTATTCATTCCTTACCAAGGTTTGATCAAGAAGTTAACGAGGAAGCATTC
AAAACAGTTCAGAAAACATCTGAGTTGCCTCTTACAATTCTTCACACAAA
TAATAAGGTTCACATGCGCACGTTGTCCGGTAACAACATATTACACAAAA
TACCCCCAACGAATAGATATTCATACAGTAGCCCAATCTCAAAAACGATG
AGCCAGCATGTATCTAAAGGCTCGTTGGATAATTTCCCAATGAAATTGGA
TAATTTAAATACACAAGAGAACAACGAAGATGAACCCGACGAGGAAGACA
ATGTTTGGGATGGTTATTCAGAGGAAGACAATAAAGGCCTATTAGGCCCA
GATGTGAAAACCGATTTCAATTTGTTCTGTCAGAGAAATCAAGATGGCGA
TTCAAACGAATACAGCAGCAGTAGTGATCATGATAGTTCGTCATTTTCAT
ACGAGTCAGATGAGGATGAGAATGCTGAAAACGCGGATGTGCAAGACACA
AATCCGACTGGATATACTCTGATGGAGGCTGTGGAAGGTCTGGCAACAGT
TCCCGGCACAACGACAGACAGTGACGACCGGATTCCAATGGCCACCCCGG
AACAGTTGGAATATTACACAAAGTGCTTTCAGACAATCCAAACCAGTTCA
GACAGTGACCAAGTGTCCGCCAGTGACGCAATAACAATATTCAGGATGCA
CAACTTACAGGATGAATGTCTCGATAGGATTCTATATTTGATCAGCATCA
CAGCAGAATCACAAATAACTCTGGTCGAATTCAGCAAAGCTCTTCATTTA
ATTACTCATGTCAAAAATGGGTTTGCATTACCGGAAACCTTGCCAGAAAA
CTTGCTTCCTGTTGAATTTGAGCAACCAGATGAACCATTTTCTTTCGACT
TGTCCGACGGTGAACTACCAGATGTGTCAAATACTTATCAAACATCATCT
GTCGAATCAATCAGTTCTGTAGATGATGAGCGATCAGGTTTTGATTTTAC
AGATGTTGCAGCTAATCGGAATTGGGTTCAGTCCAAGTCACGATTTCTAA
ACAAAAATCAGTACAGCAAATCGTCAAATTCATTTTCCCAATCAAAAACC
TCTGATAAAAAGCATTCATTAGCTAGTAATAACGAAGAAGCTGCTGATGT
TAGCAGACCCAGGCCATGTGCTAAAAAATCTTTGTCATTGTCTCAGCCGA
TTTCGAGCAGTGTAGCTCCACCACCACCTCCACGTTCTAGCGTGCCAAGT
GAAAATTTAAATAAAAAATTATCGTTTGAAAGAAATACTGACCCCTCTGG
TGATTCACTTTCAGCTGCTTCATGTGCCATTTATCCTAGGGAATCCGATG
TGCAGATTAGAAAATTTTCCGAAACTTCAAAATCTGAGATTACAAACAAT
GCCAGATTTTCAATTAATCAAGAGCCTCGTAAGCAACTGCAAATTTCTAT
GAGAGACTTGAAATCTAAAAACCATGTACTGCTGAGGTTAAACAATGAAA
TGACAAGCGAGTTGGAGGAATTGCGGGAACAGAGAGTCAATCTTCATCTT
CAATTGGAATACATCAAACAATCACAAATAGACATTCAATAATGAACAAT
GAAATTGACAAGAATCTAACAACTGTTGAGTCCAAATTGAATGCTAATTT
CAACCAATCTAATTATTCGCCGTCACTCAAAAGATAAACGCAATTTGATT
GGTGTATCAGTTTTTTCCTTGTCTTATAAATGATTATCACAGAAAATCTC
AAATTTGTCAATTTCTTATGTGTATTCTTATTATCTTTCAAATAGACTTG
GGAATTATCTTTGATGGAAATACACAGATTGAGTTTCACGACTATTATCA
TTTATAATATTATTATTTTCTATTTTTGGGTTTCTGTTAATA
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protein sequence of SMED30012449-orf-1

>SMED30012449-orf-1 ID=SMED30012449-orf-1|Name=SMED30012449-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=623bp
MTQFNIKLEETLFYEKLFAQCDVQKVGKVSGLEAYKLFLKSGIPVDDLKK
ICEYCGANRLGFFGRSQFYIALKIISLAQAGTDVDENILQKNVIHSLPRF
DQEVNEEAFKTVQKTSELPLTILHTNNKVHMRTLSGNNILHKIPPTNRYS
YSSPISKTMSQHVSKGSLDNFPMKLDNLNTQENNEDEPDEEDNVWDGYSE
EDNKGLLGPDVKTDFNLFCQRNQDGDSNEYSSSSDHDSSSFSYESDEDEN
AENADVQDTNPTGYTLMEAVEGLATVPGTTTDSDDRIPMATPEQLEYYTK
CFQTIQTSSDSDQVSASDAITIFRMHNLQDECLDRILYLISITAESQITL
VEFSKALHLITHVKNGFALPETLPENLLPVEFEQPDEPFSFDLSDGELPD
VSNTYQTSSVESISSVDDERSGFDFTDVAANRNWVQSKSRFLNKNQYSKS
SNSFSQSKTSDKKHSLASNNEEAADVSRPRPCAKKSLSLSQPISSSVAPP
PPPRSSVPSENLNKKLSFERNTDPSGDSLSAASCAIYPRESDVQIRKFSE
TSKSEITNNARFSINQEPRKQLQISMRDLKSKNHVLLRLNNEMTSELEEL
REQRVNLHLQLEYIKQSQIDIQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0006898receptor-mediated endocytosis
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
Vocabulary: INTERPRO
TermDefinition
IPR000261EH_dom
IPR011992EF-hand-dom_pair
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0005509calcium ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 583..617
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 5..102
e-value: 6.6E-18
score: 66.1
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 281..383
e-value: 2.5E-15
score: 58.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 224..239
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 446..509
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 224..260
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 446..463
NoneNo IPR availablePANTHERPTHR11216EH DOMAINcoord: 9..604
NoneNo IPR availablePANTHERPTHR11216:SF61GH06923Pcoord: 9..604
IPR000261EH domainSMARTSM00027eh_3coord: 287..383
e-value: 0.0024
score: 16.0
coord: 3..99
e-value: 4.0E-6
score: 36.3
IPR000261EH domainPFAMPF12763EF-hand_4coord: 295..379
e-value: 5.6E-5
score: 23.0
IPR000261EH domainPROSITEPS50031EHcoord: 10..98
score: 11.629
IPR000261EH domainPROSITEPS50031EHcoord: 294..379
score: 10.226
IPR000261EH domainCDDcd00052EHcoord: 14..80
e-value: 1.01963E-13
score: 64.1631
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 284..379
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 8..81