SMED30012432

Overview
NameSMED30012432
Smed IDSMED30012432
Length (bp)1746
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30012432 (SMED30012432) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30012432 (SMED30012432) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30012432 (SMED30012432) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30012432 (SMED30012432) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012432

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30012432SMESG000040165.1 SMESG000013648.1 SMESG000000064.1 Contig800GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30012432SMESG000060566.1 SMESG000060556.1 Contig800GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30012432SMESG000060566.1 SMESG000060556.1 dd_Smed_v4_4002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30012432SMESG000060566.1 SMESG000060556.1 dd_Smed_v4_4002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30012432SMESG000060566.1 SMESG000060556.1 dd_Smed_v4_4002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30012432SMESG000060566.1 SMESG000060556.1 dd_Smed_v4_4002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30012432SMESG000060566.1 SMESG000060556.1 dd_Smed_v4_4002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30012432SMESG000060566.1 SMESG000060556.1 dd_Smed_v4_4002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30012432SMESG000060566.1 SMESG000060556.1 dd_Smed_v4_4002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30012432SMESG000060566.1 SMESG000060556.1 SMESG000060487.1 dd_Smed_v6_4002_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30012432SMESG000060566.1 SMESG000060556.1 dd_Smed_v4_4002_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30012432SMESG000060566.1 SMESG000060556.1 dd_Smed_v4_4002_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30012432 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000746:6976..30164 -7814
Homology
[Close]
BLAST of SMED30012432 vs. Planmine SMEST
Match: SMESG000060566.1 (SMESG000060566.1)

HSP 1 Score: 893.264 bits (2307), Expect = 0.000e+0
Identity = 486/513 (94.74%), Postives = 488/513 (95.13%), Query Frame = 2 
Query:  128 MALFKFLKQFLILEIIWRFIVGKQLIYTEYHISSKESLSYLTRGIPERYEIHNQFEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCIRCRYRLIELTVSITDRINIQCNNQPYKIFSYSNQKLINN----------------AYPIVMTFENSQARLWLVNTPAHGYFDPNPSQLAGCIHDVIINGVPSEMERSGIEISTECPKACVNDVCNAGVCHETYDRDPFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIKISWRAHSDDILGIWKEKISLKIQVKRNHLYLLYEEKILFIKKTITNKYQPNQNDCVYLSFDANFIRIKFVQNDSTIITKLPVPKDLSKEDFTSISLLSSPGFIDIVHKLRPSQYQWLPFPSKFFVNQTDRSAIPKKTSIVFQTPQDXXXXXXXXXXDXXXXXXXXXXXXXXXXXXXXXVKYFRNKNEMKHDKPDLSTSNLLENLXXXXXXXXXXXXXXCNFDCFYT 1618
            MALFKFLKQFLILEIIWRFIVGKQLIYTEYHISSKES SYLTRGIPERYE HNQ EFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCIRCRYRLIELTVSITDRINIQCNNQPYKIFSYSNQKLINN                 YPIVMTFENSQARLWLVNTPAHGYF PNPSQLAGCIHDVIINGVPSEMER GIEISTECPKACVNDVCNAGVCHETYDRDPFCDCTDSFLNGPFCNSSSITQMDSN+KYHSNTFRSKLIKISWRAHSDDILGIWKEKISLKIQVKRNHLYLLYEEKILFIKKTITNKYQPNQNDCVYLSFDANFI IKFVQN+STIITKLPVPKDLSKEDFTSISLLSSPGFIDIVHKLRPSQYQWLPFPSKFFVNQTDRSAIPKKTSIV QTPQDSKRKRLSSSRDIAIGILSFLLLIFIILAAVFAVKYFRNKNEMKHDKPDLSTSNLLENLKDKESISKRS SKSCNFDCFYT
Sbjct:    1 MALFKFLKQFLILEIIWRFIVGKQLIYTEYHISSKESPSYLTRGIPERYEKHNQIEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCIRCRYRLIELTVSITDRINIQCNNQPYKIFSYSNQKLINNENKAFVVHLLWDSAVCTYPIVMTFENSQARLWLVNTPAHGYFYPNPSQLAGCIHDVIINGVPSEMERCGIEISTECPKACVNDVCNAGVCHETYDRDPFCDCTDSFLNGPFCNSSSITQMDSNKKYHSNTFRSKLIKISWRAHSDDILGIWKEKISLKIQVKRNHLYLLYEEKILFIKKTITNKYQPNQNDCVYLSFDANFIEIKFVQNNSTIITKLPVPKDLSKEDFTSISLLSSPGFIDIVHKLRPSQYQWLPFPSKFFVNQTDRSAIPKKTSIVLQTPQDSKRKRLSSSRDIAIGILSFLLLIFIILAAVFAVKYFRNKNEMKHDKPDLSTSNLLENLKDKESISKRSFSKSCNFDCFYT 513
[Close]
BLAST of SMED30012432 vs. Planmine SMEST
Match: SMESG000060556.1 (SMESG000060556.1)

HSP 1 Score: 875.93 bits (2262), Expect = 0.000e+0
Identity = 465/498 (93.37%), Postives = 476/498 (95.58%), Query Frame = 2 
Query:  128 MALFKFLKQFLILEIIWRFIVGKQLIYTEYHISSKESLSYLTRGIPERYEIHNQFEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCIRCRYRLIELTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQARLWLVNTPAHGYFDPNPSQLAGCIHDVIINGVPSEMERSGIEISTECPKACVNDVCNAGVCHETYDRDPFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIKISWRAHSDDILGIWKEKISLKIQVKRNHLYLLYEEKILFIKKTITNKYQPNQNDCVYLSFDANFIRIKFVQNDSTIITKLPVPKDLSKEDFTSISLLSSPGFIDIVHKLRPSQYQWLPFPSKFFVNQTDRSAIPKKTSIVFQTPQ-DXXXXXXXXXXDXXXXXXXXXXXXXXXXXXXXXVKYFRNKNEMKHDKPDLSTSNLLENLXXXXXXXXXXXXXXCNFDCFYT 1618
            M +FKFLKQFLI EIIW FIVGKQLIY EYHISSKESLSYLTRGIPERYEIHNQFEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGC+RCRYRLI+LTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQA+LWLVNTPAHGYF PNPSQLAGCIHDVIINGVPSEMERSGIEISTECPKACVNDVCNAGVCHETYDR+PFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIK SWRAHSDDILGIWKEKISLKIQVKRNHLY LYEEK LFIKKTITNKYQPNQNDCVYLSFD NFI IKFVQNDSTIITKLPVPKDLSKEDFTSISLLSSPGFIDIVHKLRPSQYQWLPFPSKFFVNQTDRSAIPKKTS+V QTPQ   ++   S SR+I IGILSFLLLIFIILAAV+AVK FR +NEMKH KPDLSTSNLLENLKDKESISKRSSSKSCNFDCFYT
Sbjct:    1 MTIFKFLKQFLIPEIIWIFIVGKQLIYAEYHISSKESLSYLTRGIPERYEIHNQFEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCVRCRYRLIDLTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQAKLWLVNTPAHGYFYPNPSQLAGCIHDVIINGVPSEMERSGIEISTECPKACVNDVCNAGVCHETYDREPFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIKTSWRAHSDDILGIWKEKISLKIQVKRNHLYFLYEEKFLFIKKTITNKYQPNQNDCVYLSFDTNFIGIKFVQNDSTIITKLPVPKDLSKEDFTSISLLSSPGFIDIVHKLRPSQYQWLPFPSKFFVNQTDRSAIPKKTSMVLQTPQYSKRKDSGSISREIVIGILSFLLLIFIILAAVYAVKIFRKRNEMKHHKPDLSTSNLLENLKDKESISKRSSSKSCNFDCFYT 498
[Close]
BLAST of SMED30012432 vs. Planmine SMEST
Match: SMESG000060556.1 (SMESG000060556.1)

HSP 1 Score: 869.381 bits (2245), Expect = 0.000e+0
Identity = 461/500 (92.20%), Postives = 473/500 (94.60%), Query Frame = 2 
Query:  128 MALFKFLKQFLILEIIWRFIVGKQLIYTEYHISSKESLSYLTRGIPERYEIHNQFEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCIRCRYRLIELTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQARLWLVNTPAHGYFDPNPSQLAGCIHDVIINGVPSEMERSGIEISTECPKACVNDVCNAGVCHETYDRDPFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIKISWRAHSDDILGIWKEKISLKIQVKRNHLYLLYEEKILFIKKTITNKYQPNQNDCVYLSFDANFIRIKFVQNDSTIITKLPVPKDLSKEDFTSISLLSSPGFIDIVHKLRPSQYQWLPFPSKFFVNQTDRSAIPKKTSIVFQTPQ-DXXXXXXXXXXDXXXXXXXXXXXXXXXXXXXXXVKYFRNKNEMKHDKPDLSTSNLLENLXXXXXXXXXXXXXXCNFDCFYT*D 1624
            M +FKFLKQFLI EIIW FIVGKQLIY EYHISSKESLSYLTRGIPERYEIHNQFEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGC+RCRYRLI+LTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQA+LWLVNTPAHGYF PNPSQLAGCIHDVIINGVPSEMERSGIEISTECPKACVNDVCNAGVCHETYDR+PFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIK SWRAHSDDILGIWKEKISLKIQVKRNHLY LYEEK LFIKKTITNKYQPNQNDCVYLSFD NFI IKFVQNDSTIITKLPVPKDLSKEDFTSISLLSSPGFIDIVHKLRPSQYQWLPFPSKFFVNQTDRSAIPKKTS+V QTPQ   ++   S SR+I IGILSFLLLIFIILAAV+AVK FR +NEMKH KPDLSTSNLLENLKDKESISKRS SKSCNFD   + D
Sbjct:    1 MTIFKFLKQFLIPEIIWIFIVGKQLIYAEYHISSKESLSYLTRGIPERYEIHNQFEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCVRCRYRLIDLTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQAKLWLVNTPAHGYFYPNPSQLAGCIHDVIINGVPSEMERSGIEISTECPKACVNDVCNAGVCHETYDREPFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIKTSWRAHSDDILGIWKEKISLKIQVKRNHLYFLYEEKFLFIKKTITNKYQPNQNDCVYLSFDTNFIGIKFVQNDSTIITKLPVPKDLSKEDFTSISLLSSPGFIDIVHKLRPSQYQWLPFPSKFFVNQTDRSAIPKKTSMVLQTPQYSKRKDSGSISREIVIGILSFLLLIFIILAAVYAVKIFRKRNEMKHHKPDLSTSNLLENLKDKESISKRSFSKSCNFDSLQSYD 500
[Close]
BLAST of SMED30012432 vs. Planmine SMEST
Match: SMESG000060556.1 (SMESG000060556.1)

HSP 1 Score: 827.78 bits (2137), Expect = 0.000e+0
Identity = 440/475 (92.63%), Postives = 452/475 (95.16%), Query Frame = 2 
Query:  203 IYTEYHISSKESLSYLTRGIPERYEIHNQFEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCIRCRYRLIELTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQARLWLVNTPAHGYFDPNPSQLAGCIHDVIINGVPSEMERSGIEISTECPKACVNDVCNAGVCHETYDRDPFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIKISWRAHSDDILGIWKEKISLKIQVKRNHLYLLYEEKILFIKKTITNKYQPNQNDCVYLSFDANFIRIKFVQNDSTIITKLPVPKDLSKEDFTSISLLSSPGFIDIVHKLRPSQYQWLPFPSKFFVNQTDRSAIPKKTSIVFQTPQ-DXXXXXXXXXXDXXXXXXXXXXXXXXXXXXXXXVKYFRNKNEMKHDKPDLSTSNLLENLXXXXXXXXXXXXXXCNFDCFYT*D 1624
            IY+EYHISSKESLSYLTRGIPERYEIHNQFEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGC+RCRYRLI+LTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQA+LWLVNTPAHGYF PNPSQLAGCIHDVIINGVPSEMERSGIEISTECPKACVNDVCNAGVCHETYDR+PFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIK SWRAHSDDILGIWKEKISLKIQVKRNHLY LYEEK LFIKKTITNKYQPNQNDCVYLSFD NFI IKFVQNDSTIITKLPVPKDLSKEDFTSISLLSSPGFIDIVHKLRPSQYQWLPFPSKFFVNQTDRSAIPKKTS+V QTPQ   ++   S SR+I IGILSFLLLIFIILAAV+AVK FR +NEMKH KPDLSTSNLLENLKDKESISKRS SKSCNFD   + D
Sbjct:    3 IYSEYHISSKESLSYLTRGIPERYEIHNQFEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCVRCRYRLIDLTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQAKLWLVNTPAHGYFYPNPSQLAGCIHDVIINGVPSEMERSGIEISTECPKACVNDVCNAGVCHETYDREPFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIKTSWRAHSDDILGIWKEKISLKIQVKRNHLYFLYEEKFLFIKKTITNKYQPNQNDCVYLSFDTNFIGIKFVQNDSTIITKLPVPKDLSKEDFTSISLLSSPGFIDIVHKLRPSQYQWLPFPSKFFVNQTDRSAIPKKTSMVLQTPQYSKRKDSGSISREIVIGILSFLLLIFIILAAVYAVKIFRKRNEMKHHKPDLSTSNLLENLKDKESISKRSFSKSCNFDSLQSYD 477
[Close]
BLAST of SMED30012432 vs. Planmine SMEST
Match: SMESG000060556.1 (SMESG000060556.1)

HSP 1 Score: 585.104 bits (1507), Expect = 0.000e+0
Identity = 276/287 (96.17%), Postives = 281/287 (97.91%), Query Frame = 2 
Query:  128 MALFKFLKQFLILEIIWRFIVGKQLIYTEYHISSKESLSYLTRGIPERYEIHNQFEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCIRCRYRLIELTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQARLWLVNTPAHGYFDPNPSQLAGCIHDVIINGVPSEMERSGIEISTECPKACVNDVCNAGVCHETYDRDPFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIKISWRAHSDDILGIWKE 988
            M +FKFLKQFLI EIIW FIVGKQLIY EYHISSKESLSYLTRGIPERYEIHNQFEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGC+RCRYRLI+LTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQA+LWLVNTPAHGYF PNPSQLAGCIHDVIINGVPSEMERSGIEISTECPKACVNDVCNAGVCHETYDR+PFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIK SWRAHSDDILGIWKE
Sbjct:    1 MTIFKFLKQFLIPEIIWIFIVGKQLIYAEYHISSKESLSYLTRGIPERYEIHNQFEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCVRCRYRLIDLTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTFENSQAKLWLVNTPAHGYFYPNPSQLAGCIHDVIINGVPSEMERSGIEISTECPKACVNDVCNAGVCHETYDREPFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIKTSWRAHSDDILGIWKE 287
The following BLAST results are available for this feature:
BLAST of SMED30012432 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012432 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012432 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012432 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012432 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012432 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012432 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012432 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012432 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012432 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012432 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012432 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012432 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012432 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000060566.10.000e+094.74SMESG000060566.1[more]
SMESG000060556.10.000e+093.37SMESG000060556.1[more]
SMESG000060556.10.000e+092.20SMESG000060556.1[more]
SMESG000060556.10.000e+092.63SMESG000060556.1[more]
SMESG000060556.10.000e+096.17SMESG000060556.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012432 ID=SMED30012432|Name=SMED30012432|organism=Schmidtea mediterranea sexual|type=transcript|length=1746bp
GTCGAGCATTTTAATCTTTACTAAAGATAGTTTTAAAAATAACTATAATG
CGCATTAAGTAATTAAACTCATTTTTTGTCACACCATTAAATATGTTATC
GTTTCATTATAAAAAACTAACAATTTCATGGCATTATTTAAATTTCTGAA
GCAGTTTTTAATTCTGGAAATCATATGGAGATTTATTGTAGGGAAGCAAC
TAATATATACCGAATACCACATCAGTAGCAAGGAGAGTCTTAGCTATTTG
ACCAGAGGTATTCCAGAAAGATATGAAATACATAATCAATTTGAATTTAA
AATGACTCTAAATAGGATGGAAGGAACTTTATTATATATGTATGATAAAT
ATGACAATCAATTGATAATTTCCCTTTCTGATGATCTGGAAGAGAGAATG
AAAATAGAATTCAGGGGTAAAGAACACCATTTAACTCTATATGAATTGTG
TGGATGCATCAGATGTAGATATCGATTGATCGAGCTGACCGTGTCCATCA
CTGATCGGATCAATATCCAATGCAATAATCAGCCATATAAAATATTCAGC
TATTCCAATCAGAAATTGATTAATAATGCTTATCCGATCGTGATGACATT
TGAAAATTCCCAAGCGAGATTATGGCTTGTAAACACTCCTGCTCATGGTT
ACTTTGATCCGAACCCTTCACAGTTGGCAGGCTGCATTCATGATGTAATA
ATAAATGGAGTTCCTTCGGAAATGGAGAGAAGTGGCATTGAGATATCCAC
TGAATGTCCAAAGGCGTGCGTCAATGACGTGTGCAATGCTGGCGTGTGCC
ATGAAACATATGATAGAGATCCATTCTGTGATTGCACTGATTCATTCCTG
AACGGCCCATTCTGTAATTCAAGCAGCATCACCCAAATGGATTCCAATAG
GAAATATCATTCAAATACTTTCCGGTCGAAATTAATCAAAATATCATGGC
GGGCTCATTCTGATGATATTTTGGGCATTTGGAAAGAAAAGATTTCACTA
AAGATTCAAGTTAAAAGAAATCATCTTTACTTACTATATGAAGAAAAAAT
TTTATTTATAAAAAAAACGATAACTAATAAATATCAACCAAATCAAAATG
ACTGCGTTTATTTATCATTTGATGCAAACTTTATCAGAATAAAATTCGTA
CAAAACGATTCTACAATAATTACGAAACTACCTGTTCCCAAAGATTTATC
TAAAGAGGATTTCACATCAATCTCATTGCTAAGCAGTCCAGGTTTCATTG
ACATCGTCCACAAATTGCGGCCTTCTCAATACCAATGGCTGCCCTTCCCT
AGCAAATTCTTTGTCAACCAAACTGATCGATCCGCCATTCCCAAAAAAAC
CAGCATAGTTTTTCAAACACCGCAAGATTCTAAGAGAAAGAGGTTATCAA
GTAGCAGAGACATCGCCATTGGAATTTTATCTTTTCTTTTGTTGATATTT
ATTATTTTGGCTGCCGTTTTCGCTGTGAAATACTTTAGGAATAAGAATGA
GATGAAACATGATAAGCCTGATCTGTCGACAAGTAATTTACTGGAGAACC
TTAAAGATAAAGAAAGCATTTCCAAGAGATCTTCCTCCAAAAGTTGCAAT
TTTGATTGCTTTTATACATGAGATTTTGATAGAAATTATTTGTATTTTAT
ACTTGTTTTTTATTATTATGATCATACAGATTGTTATTATTTATTATTAT
TTTAACTGTTAATTAATTTTGAAATGAAAGAATAAATATCTGCCTG
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protein sequence of SMED30012432-orf-1

>SMED30012432-orf-1 ID=SMED30012432-orf-1|Name=SMED30012432-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=498bp
MALFKFLKQFLILEIIWRFIVGKQLIYTEYHISSKESLSYLTRGIPERYE
IHNQFEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEH
HLTLYELCGCIRCRYRLIELTVSITDRINIQCNNQPYKIFSYSNQKLINN
AYPIVMTFENSQARLWLVNTPAHGYFDPNPSQLAGCIHDVIINGVPSEME
RSGIEISTECPKACVNDVCNAGVCHETYDRDPFCDCTDSFLNGPFCNSSS
ITQMDSNRKYHSNTFRSKLIKISWRAHSDDILGIWKEKISLKIQVKRNHL
YLLYEEKILFIKKTITNKYQPNQNDCVYLSFDANFIRIKFVQNDSTIITK
LPVPKDLSKEDFTSISLLSSPGFIDIVHKLRPSQYQWLPFPSKFFVNQTD
RSAIPKKTSIVFQTPQDSKRKRLSSSRDIAIGILSFLLLIFIILAAVFAV
KYFRNKNEMKHDKPDLSTSNLLENLKDKESISKRSSSKSCNFDCFYT*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0000106ciliated epithelial cell
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR013320ConA-like_dom_sf
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 428..450