SMED30012426

Overview
NameSMED30012426
Smed IDSMED30012426
Length (bp)3899
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30012426 (SMED30012426) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30012426 (SMED30012426) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30012426 (SMED30012426) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30012426 (SMED30012426) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012426

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
muscle cellSMED30012426SMESG000081547.1 dd_Smed_v4_12193_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30012426 vs. TrEMBL
Match: G7YIH2 (RUN and SH3 domain-containing protein 1 OS=Clonorchis sinensis OX=79923 GN=CLF_108767 PE=4 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 1.371e-18
Identity = 92/299 (30.77%), Postives = 139/299 (46.49%), Query Frame = 2 
Query: 1700 KSNKIATDHFKR--KSVSFDCP--------GLSSEHIMQEMKEIPKKWGLLGSESRLIGDMMAVFECGRRDSLVGASGLSENCSG-----NDDATRFRGMVNEVLRAVKQISDRLSVGTMNGIEKPCSLEMNSSFQSQLAILCEMVSNGDGLESPLATAIDNLITLMSDGLVVTSFSNKKVRISSSSANKAAMRLWLLVEETCRNPGSQNTSRKAELAQLLREVRQIITNISSTKDHQLRLRAFIANALKSGLLHVWIRSVVANETALQRFYCPEAFLRQARSGQSDALAALTVSLKAL 2551
            KS  + T H +R  KSVSF           G+ S     E K IP+ WGL G E+ L        E   R+S     G   N  G     +D   R+  MVN+V++AV++     S  T +G   P     N++     + +  + S G     PL   +  L  L+SDGL+  +   K + I+     K   RLW +VEE+CR PG   +       ++L +    +  ++S    +++ +AFI   L +  L +W+ +VVANE+ L RFYC +AF+RQ R+ Q    A L   L+ L
Sbjct:  978 KSYDLFTPHGRRPKKSVSFSGKIRLLRVQNGVESP-TSPEPKVIPRNWGLYGQETPLA-------EFNPRNS----PGRDTNQPGEVPRQSDVDVRYHAMVNDVIKAVQETVAYYSTAT-SGNPDPAGNGCNAADPLPYSAIAALGSAG---RQPLLAVVAPLTVLLSDGLLPPT---KPIFIT-----KPRTRLWQMVEESCR-PGPLLS---GVAYRVLSDAVSQVKALTSITLEKVKFKAFICACLNAKALPMWLNAVVANESLLSRFYCEDAFVRQCRATQRGLHADLLTHLEQL 1248
[Close]
BLAST of SMED30012426 vs. TrEMBL
Match: A0A419PEM5 (RUN and SH3 domain-containing protein 1 OS=Clonorchis sinensis OX=79923 GN=RUSC1 PE=4 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 1.395e-18
Identity = 92/299 (30.77%), Postives = 139/299 (46.49%), Query Frame = 2 
Query: 1700 KSNKIATDHFKR--KSVSFDCP--------GLSSEHIMQEMKEIPKKWGLLGSESRLIGDMMAVFECGRRDSLVGASGLSENCSG-----NDDATRFRGMVNEVLRAVKQISDRLSVGTMNGIEKPCSLEMNSSFQSQLAILCEMVSNGDGLESPLATAIDNLITLMSDGLVVTSFSNKKVRISSSSANKAAMRLWLLVEETCRNPGSQNTSRKAELAQLLREVRQIITNISSTKDHQLRLRAFIANALKSGLLHVWIRSVVANETALQRFYCPEAFLRQARSGQSDALAALTVSLKAL 2551
            KS  + T H +R  KSVSF           G+ S     E K IP+ WGL G E+ L        E   R+S     G   N  G     +D   R+  MVN+V++AV++     S  T +G   P     N++     + +  + S G     PL   +  L  L+SDGL+  +   K + I+     K   RLW +VEE+CR PG   +       ++L +    +  ++S    +++ +AFI   L +  L +W+ +VVANE+ L RFYC +AF+RQ R+ Q    A L   L+ L
Sbjct:  978 KSYDLFTPHGRRPKKSVSFSGKIRLLRVQNGVESP-TSPEPKVIPRNWGLYGQETPLA-------EFNPRNS----PGRDTNQPGEVPRQSDVDVRYHAMVNDVIKAVQETVAYYSTAT-SGNPDPAGNGCNAADPLPYSAIAALGSAG---RQPLLAVVAPLTVLLSDGLLPPT---KPIFIT-----KPRTRLWQMVEESCR-PGPLLS---GVAYRVLSDAVSQVKALTSITLEKVKFKAFICACLNAKALPMWLNAVVANESLLSRFYCEDAFVRQCRATQRGLHADLLTHLEQL 1248
[Close]
BLAST of SMED30012426 vs. TrEMBL
Match: A0A183PQP1 (RUN domain-containing protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS16676 PE=4 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 3.314e-18
Identity = 78/251 (31.08%), Postives = 119/251 (47.41%), Query Frame = 2 
Query: 1799 IPKKWGLLGSESRLIGDMMAVFECGRRDSLVGASGLSENCSGNDDATRFRGMVNEVLRAVKQISDRLSVGTMNGIEKPCSLEMNSSFQSQLAILCEMVSNGDGLESPLATAIDNLITLMSDGLVVTSFSNKKVRISSSSANKAAMRLWLLVEETCRNPGSQNTSRKAELAQLLREVRQIITNISSTKDHQLRLRAFIANALKSGLLHVWIRSVVANETALQRFYCPEAFLRQARSGQSDALAALTVSLKAL 2551
            IP+ WGL G E  L     A F      SL G     ++   +D   R+  MVN+V++AV++         MN ++    L  +SS  +      ++ + G   + PL   I  L  L+ DGL+  S   K +  S     +   RLW LVEE+CR PG+  T     +        + +TNI+  K    + + FI   L +  L +W+ ++VAN+T L+RFYC  AF+RQ R+ Q +  A L   L+ L
Sbjct:   10 IPRNWGLYGQEQPL-----AEFNPSCSPSLDGEKN-GKSTHHSDIDVRYHAMVNDVIKAVQETVAYYCNPNMN-LKNHEELNGHSSLANTSLPYSKVEAIGSSGKQPLLAVIPPLTILLCDGLLPPS---KPIFTS-----RPRTRLWQLVEESCR-PGALPTGVAYHVLNDAISQVKALTNITLEKT---KFKGFICACLNAKALPMWLNALVANDTLLRRFYCENAFIRQCRASQRELHADLMTHLEQL 241
[Close]
BLAST of SMED30012426 vs. TrEMBL
Match: A0A4Z2D3M4 (RUN and SH3 domain-containing protein isoform 1 OS=Schistosoma japonicum OX=6182 GN=EWB00_004910 PE=4 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 1.054e-17
Identity = 94/299 (31.44%), Postives = 138/299 (46.15%), Query Frame = 2 
Query: 1706 NKIATDHFK-----RKSVSF-DCPGLSSEHIMQ---EMKEIPKKWGLLGSESRLIGDMMAVFE--CGRRDSLVGASGLSENCSG-----NDDATRFRGMVNEVLRAVKQISDRLSVGTMNGIEKPCSLEMNSSFQSQLAILCEMVSNGDGLESPLATAIDNLITLMSDGLVVTSFSNKKVRISSSSANKAAMRLWLLVEETCRNPGSQNTSRKAE-LAQLLREVRQIITNISSTKDHQLRLRAFIANALKSGLLHVWIRSVVANETALQRFYCPEAFLRQARSGQSDALAALTVSLKAL 2551
            N +  D F      +KSVSF D     S+   Q   E K IP+ WGL G E  L     A F   C        +  L ++ +G     +D   R+  MVN+V++AV++            +   C+  MN+  +   AI       G     PL   +  L  L+ DGL+  S   K +  S     +   RLW LVEE+CR PGS  T      L   + +V+ + TN++  K    + + FI   L +  L +W+ ++VAN+T L+RFYC +AF+RQ RSGQ +  A L   L+ L
Sbjct:  957 NGLVGDSFTFNSRPKKSVSFSDSHNHYSDTSAQPSPEPKVIPRNWGLYGQEQPL-----AEFNPRC--------SPSLDDDKTGKLTRRSDIDVRYHAMVNDVIKAVQET-----------VAYYCNPNMNTKIE---AI-------GSSGRQPLLAVVPPLTILLCDGLLPPS---KPIFTS-----RPRTRLWQLVEESCR-PGSLPTGVAYHVLNDAISQVKSL-TNVTLEK---TKFKGFICACLNAKALPMWLNALVANDTLLRRFYCEDAFIRQCRSGQRELHADLMTHLEQL 1208
[Close]
BLAST of SMED30012426 vs. TrEMBL
Match: A0A1S8WVM8 (RUN domain protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=X801_05661 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 1.274e-17
Identity = 89/299 (29.77%), Postives = 135/299 (45.15%), Query Frame = 2 
Query: 1700 KSNKIATDHFKR--KSVSFDCP--------GLSSEHIMQEMKEIPKKWGLLGSESRLIGDMMAVFECGRRDSLVGASGLSENCSG-----NDDATRFRGMVNEVLRAVKQISDRLSVGTMNGIEKPCSLEMNSSFQSQLAILCEMVSNGDGLESPLATAIDNLITLMSDGLVVTSFSNKKVRISSSSANKAAMRLWLLVEETCRNPGSQNTSRKAELAQLLREVRQIITNISSTKDHQLRLRAFIANALKSGLLHVWIRSVVANETALQRFYCPEAFLRQARSGQSDALAALTVSLKAL 2551
            KS  + T H +R  KSVSF           G+ S     E K IP+ WGL G E+ L        E   R+S     G   N  G     +D   R+  MVN+V++AV++     S  T +  +       N    +       + + G     PL   +  L  L+SDGL+  +   K + I+     K   RLW +VEE+CR PG   +       ++L +    +  ++S    +++ +AFI   L +  L +W+ +VVANE+ L RFYC +AF+RQ R+ Q    A L   L+ L
Sbjct: 1054 KSYDLFTPHGRRPKKSVSFSGKIRLLRVQNGVESP-TSPEPKVIPRNWGLYGQETPLA-------EFNPRNS----PGRDTNQPGELPRQSDVDVRYHAMVNDVIKAVQETVAYYSTATTSNPDA----AGNGCTTADPLPYSAIAALGSAGRQPLLAVVGPLTVLLSDGLLPPT---KPIFIT-----KPRTRLWQMVEESCR-PGPLLS---GVAYRVLSDAVSQVKALTSITLEKVKFKAFICACLNAKALPMWLNAVVANESLLSRFYCEDAFVRQCRATQRGLHADLLTHLEQL 1324
[Close]
BLAST of SMED30012426 vs. Planmine SMEST
Match: SMESG000081547.1 (SMESG000081547.1)

HSP 1 Score: 921.383 bits (2380), Expect = 0.000e+0
Identity = 464/466 (99.57%), Postives = 466/466 (100.00%), Query Frame = 2 
Query: 1649 LRKEDDSDESKEVICRPKSNKIATDHFKRKSVSFDCPGLSSEHIMQEMKEIPKKWGLLGSESRLIGDMMAVFECGRRDSLVGASGLSENCSGNDDATRFRGMVNEVLRAVKQISDRLSVGTMNGIEKPCSLEMNSSFQSQLAILCEMVSNGDGLESPLATAIDNLITLMSDGLVVTSFSNKKVRISSSSANKAAMRLWLLVEETCRNPGSQNTSRKAELAQLLREVRQIITNISSTKDHQLRLRAFIANALKSGLLHVWIRSVVANETALQRFYCPEAFLRQARSGQSDALAALTVSLKALTSTQNSQTPLYNNQSAPIANKLGLPPPVRISRRFFSSPTAEISNKVSPNHSQKPLYSEENTESESCADESRSSQSECPPNRDISNNNNIFSRATHPRTVKKXXXXXXXXXXXXXXRNFPFRSPAKSLTNQNFNSPLKPSVVSISMKTIRTQPIASRIKPSKTDKS 3046
            LRKEDDSDESKEVICRPKSNKIATDHFKRKSVSFDCPGLSSEHIMQEMKEIPKKWGLLGSESRLIGDMMAVFECGRRDSLVGASGLSENCSGNDDATRFRGMVNEVLRAVKQISDRLSVGTMNGIEKPCSLEMNSSFQSQLAILCEMVSNGDGLESPLATAIDNLITLMSDGLVVTSFSNKKVRISSSSANKAAMRLWLLVEETCRNPGSQNTSRKAELAQLLREVRQIITNISSTKDHQLRLRAFIANALKSGLLHVWIRSVVANETALQRFYCPEAFLRQARSGQSDALAALTVSLKALTSTQNSQTPLYNNQSAPIANKLGLPPPVRISRRFFSSPTAEISNKVSPNHSQKPLYSEENTESESCADESRSSQSECPPNRDISNNNNIFSRATHPRTVKKSNSSQLHSTTSDSSRNFPFRSPAKSLTNQNFN+PLKPSVVSISMKTIRTQPIASRIKP+KTDKS
Sbjct:  463 LRKEDDSDESKEVICRPKSNKIATDHFKRKSVSFDCPGLSSEHIMQEMKEIPKKWGLLGSESRLIGDMMAVFECGRRDSLVGASGLSENCSGNDDATRFRGMVNEVLRAVKQISDRLSVGTMNGIEKPCSLEMNSSFQSQLAILCEMVSNGDGLESPLATAIDNLITLMSDGLVVTSFSNKKVRISSSSANKAAMRLWLLVEETCRNPGSQNTSRKAELAQLLREVRQIITNISSTKDHQLRLRAFIANALKSGLLHVWIRSVVANETALQRFYCPEAFLRQARSGQSDALAALTVSLKALTSTQNSQTPLYNNQSAPIANKLGLPPPVRISRRFFSSPTAEISNKVSPNHSQKPLYSEENTESESCADESRSSQSECPPNRDISNNNNIFSRATHPRTVKKSNSSQLHSTTSDSSRNFPFRSPAKSLTNQNFNNPLKPSVVSISMKTIRTQPIASRIKPAKTDKS 928

HSP 2 Score: 820.076 bits (2117), Expect = 0.000e+0
Identity = 461/463 (99.57%), Postives = 461/463 (99.57%), Query Frame = 1 
Query:   13 MDILVESIEYYYDEDFSVPFDIGIXXXXXXXXXXXDYQCENILKYVXXXXXXXXXXXXXXXXXXXRILSPDIFSPNKLNNMQNQHKNIKFINLRHSYDNDFKRPESTQSAVRMXXXXXXXXXXXXXMATNNIDKCNPTYIDTQTVSFNESIYSLQSRVPLFNVNNKNILVNYHLHFAPQDSFINSSYVTSNCAENGIIEQKFKQISNDKHSSSSNLIDSGKYSGTTVDSILQHITEQKPLFLRETSTNNQTLKPDSILWRQIKFLMNYYYTGWRLLKMFKQFRKKVKTEPYSTDEIVFYRTNSQLKRKQSYSCSVLEIFNSIKDCGKRLENNSSKSDEQKLLVNKPIQNFSCQFPPKHRVPNQRNHITSPQRTNNQIYDYPGIGRIGMPKPTRLSSSAFLTXXXXXXXXXXXXXXTNHHSDITSNRLYDGDQISQSPRSLCSLNCQKNQSKNSDATHTLKPCL 1401
            MDILVESIEYYYDEDFSVPFDIGITEENNNTNLNLDYQCENILKYVDITDDEENDPDDYDIEDFERILSPDIFSPNKLNNMQNQHKNIKFINLRHSYDNDFKRPESTQSAVRMSCSSSSSPISSSSMATNNIDKCNPTYIDTQTVSFNESIYSLQSRVPLFNVNNKNILVNYHLHFAPQDSFINSSYV SNCAENGIIEQKFKQISNDKHSSSSNLIDSGKYSGTTVDSILQHITEQKPLFLRETSTNNQTLKPDSILWRQIKFLMNYYYTGWRLLKMFKQFRKKVKTEPYSTDEIVFYRTNSQLKRKQSYSCSVLEIFNSIKDCGKRLENNSSKSDEQKLLVNKPIQNFSCQFPPKHRVPNQRNHI SPQRTNNQIYDYPGIGRIGMPKPTRLSSSAFLTSNLHSHLSFLQSSLTNHHSDITSNRLYDGDQISQSPRSLCSLNCQKNQSKNSDATHTLKPCL
Sbjct:    1 MDILVESIEYYYDEDFSVPFDIGITEENNNTNLNLDYQCENILKYVDITDDEENDPDDYDIEDFERILSPDIFSPNKLNNMQNQHKNIKFINLRHSYDNDFKRPESTQSAVRMSCSSSSSPISSSSMATNNIDKCNPTYIDTQTVSFNESIYSLQSRVPLFNVNNKNILVNYHLHFAPQDSFINSSYVASNCAENGIIEQKFKQISNDKHSSSSNLIDSGKYSGTTVDSILQHITEQKPLFLRETSTNNQTLKPDSILWRQIKFLMNYYYTGWRLLKMFKQFRKKVKTEPYSTDEIVFYRTNSQLKRKQSYSCSVLEIFNSIKDCGKRLENNSSKSDEQKLLVNKPIQNFSCQFPPKHRVPNQRNHIISPQRTNNQIYDYPGIGRIGMPKPTRLSSSAFLTSNLHSHLSFLQSSLTNHHSDITSNRLYDGDQISQSPRSLCSLNCQKNQSKNSDATHTLKPCL 463
[Close]
BLAST of SMED30012426 vs. Planmine SMEST
Match: SMESG000078693.1 (SMESG000078693.1)

HSP 1 Score: 136.346 bits (342), Expect = 2.204e-32
Identity = 97/286 (33.92%), Postives = 160/286 (55.94%), Query Frame = 2 
Query: 1787 EMKEIPKKWGLLGSESRLIGDMMAVFECGRRDSLVGASGLSENCSGNDDATRFR--GMVNEVLRAVKQISDRLSVGTMNGIEKPCSLEM---NSSFQSQLAILCEMVSNGDGLESPLATAIDNLITLMSDGLVVTSFSNKKVRISSSSANKAAMRLWLLVEETCRNPGSQNTSRKAELAQLLREVRQ---IITNISSTKDH--QLRLRAFIANALKSGLLHVWIRSVVANETALQRFYCPEAFLRQARSGQSDALAALTVSLKALTSTQNSQTPLYNNQSAPIANK 2614
            +++ IP+ WGL         D++A+FE  RR S      + E     D+   FR  G+VN  + A+K  S+  SV ++  +   C   +   +S+ QS  A+L                A+D +I L+SDGL   S  NKK +   ++  K   +LW+ VEE+CR    ++ S+K  L+ LL++V+Q   + + +S +K+   QL+L+ FI  A+K+ LL+VW+++VVAN+T L RF+   +FLRQAR+  S+AL+A+ V ++ L    +S+  +Y N S PI++K
Sbjct:  455 DLRPIPRNWGLSKD-----NDILAMFEFPRRSS--NDFKVVEY----DEENNFRLGGIVNNAITAIKLASE--SVSSIQEVATICDKFIRCKDSNRQSTSALLL---------------AVDTIIALISDGLYSDSMRNKKSQTVLATGFKMTQKLWMFVEESCRGTLIKDPSQKLALSNLLKQVKQTCGLTSTVSRSKEWAIQLKLQIFIVLAIKNNLLYVWVKAVVANDTILHRFFNSNSFLRQARTSYSNALSAVVVEMRHLCMPGSSR--IY-NVSKPISSK 709
[Close]
BLAST of SMED30012426 vs. Planmine SMEST
Match: SMESG000056880.1 (SMESG000056880.1)

HSP 1 Score: 65.855 bits (159), Expect = 1.755e-10
Identity = 67/272 (24.63%), Postives = 116/272 (42.65%), Query Frame = 2 
Query: 1793 KEIPKKWGLLGSESRLIGDMMAVFECGRRDSLVGAS--GLSENCSGNDDATRFRGMVNEVLRAVKQISDRLSVGTMNGIEKPCSLEMNSSFQSQLAILCEMVSNGDGLESPLATAIDNLITLMSDGLVVTSFSNKKVRISSSSANKAAMRLWLLVEETCRNPGSQNTSRKAELAQLLREVRQI-ITNISSTKDHQLRLRAFIANALKSGLLHVWIRSVVANETALQRFYCPEAFLRQARSGQSDALAALTVSLKAL--------TSTQNSQT 2575
            +EIP+ WGL G+ S +I +            ++GA      EN   N+D  +++ MV ++++AV++     S         P S  + + F         M+             +D L  L++DG++   +    + I      K+  RLW ++E    N    N         +++ V++I ITN       + R + FI++ + + LL  W   +V NE  L+  Y  E+FLRQ  +      A L  +L+ L        T T++SQT
Sbjct:  574 QEIPRDWGLYGNGSVMISEF-----------VLGAQLKYSKENELSNEDL-KYKVMVRDIIKAVQETIANCSDQAQENKTLPESKTIKAPF---------MI-------------VDPLYVLLTDGIL--PYQKNVITI------KSRYRLWNVLETNSNNAEYMN--------DIMKAVKKIPITN------EKNRFKFFISHLINAKLLQRWFNDLVNNENLLKNNYSKESFLRQVNTNLKGLYADLDTNLQLLDRLPYGNSTKTKSSQT 789

HSP 2 Score: 56.6102 bits (135), Expect = 1.413e-7
Identity = 49/200 (24.50%), Postives = 86/200 (43.00%), Query Frame = 1 
Query:  736 LRETSTNNQTLKPDSILWRQIKFLMNYYYTGWRLLKMFKQF--------------RKKVKTEP----------YSTDEIVFYRTNSQLKRKQSYSCSVLEIFNSIKD-------------CGKRLENNSSKSDEQKLLVNKPIQNFSCQFPPKHRV---PNQRNHITSPQRTNNQIYDYPGIGRIGMPKPTRLSSSAFLT 1215
            L +T  +   ++ +S  WRQ+K   +     W  LK   +               R +   +P          ++++E V YR +S+ ++   +S ++LEI++  +              C  ++ N S+K+          + NF  QFPP       P QR++    ++  N    YPG+G I  PK TRL++SAFL+
Sbjct:  186 LLKTKNDENNIEGNSTTWRQLKDNASNKVISWNKLKNATKMPDDNLLNKTKDNNSRSRSVIKPNIIKMDDSLRFTSNENVKYRAHSEPRKSHRFSGTLLEIYSRTRGLNQSKNSQEFFELCENKVHNESTKT----------LVNFGAQFPPTAATDFPPRQRSNSLLEKKAQN----YPGLGLIKTPKSTRLTNSAFLS 371
[Close]
BLAST of SMED30012426 vs. Planmine SMEST
Match: SMESG000056880.1 (SMESG000056880.1)

HSP 1 Score: 56.9954 bits (136), Expect = 9.929e-8
Identity = 49/200 (24.50%), Postives = 86/200 (43.00%), Query Frame = 1 
Query:  736 LRETSTNNQTLKPDSILWRQIKFLMNYYYTGWRLLKMFKQF--------------RKKVKTEP----------YSTDEIVFYRTNSQLKRKQSYSCSVLEIFNSIKD-------------CGKRLENNSSKSDEQKLLVNKPIQNFSCQFPPKHRV---PNQRNHITSPQRTNNQIYDYPGIGRIGMPKPTRLSSSAFLT 1215
            L +T  +   ++ +S  WRQ+K   +     W  LK   +               R +   +P          ++++E V YR +S+ ++   +S ++LEI++  +              C  ++ N S+K+          + NF  QFPP       P QR++    ++  N    YPG+G I  PK TRL++SAFL+
Sbjct:  186 LLKTKNDENNIEGNSTTWRQLKDNASNKVISWNKLKNATKMPDDNLLNKTKDNNSRSRSVIKPNIIKMDDSLRFTSNENVKYRAHSEPRKSHRFSGTLLEIYSRTRGLNQSKNSQEFFELCENKVHNESTKT----------LVNFGAQFPPTAATDFPPRQRSNSLLEKKAQN----YPGLGLIKTPKSTRLTNSAFLS 371
The following BLAST results are available for this feature:
BLAST of SMED30012426 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012426 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012426 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012426 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012426 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012426 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012426 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012426 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
G7YIH21.371e-1830.77RUN and SH3 domain-containing protein 1 OS=Clonorc... [more]
A0A419PEM51.395e-1830.77RUN and SH3 domain-containing protein 1 OS=Clonorc... [more]
A0A183PQP13.314e-1831.08RUN domain-containing protein OS=Schistosoma matth... [more]
A0A4Z2D3M41.054e-1731.44RUN and SH3 domain-containing protein isoform 1 OS... [more]
A0A1S8WVM81.274e-1729.77RUN domain protein (Fragment) OS=Opisthorchis vive... [more]
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BLAST of SMED30012426 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012426 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012426 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012426 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012426 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012426 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000081547.10.000e+099.57SMESG000081547.1[more]
SMESG000078693.12.204e-3233.92SMESG000078693.1[more]
SMESG000056880.11.755e-1024.63SMESG000056880.1[more]
SMESG000056880.19.929e-824.50SMESG000056880.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012426 ID=SMED30012426|Name=SMED30012426|organism=Schmidtea mediterranea sexual|type=transcript|length=3899bp
AAATCTTATATGATGGATATTTTAGTAGAAAGTATTGAATATTATTATGA
TGAGGATTTTTCTGTTCCATTTGATATTGGTATAACCGAGGAAAACAATA
ATACGAATTTGAACCTTGATTATCAATGTGAGAATATATTAAAATATGTT
GATATTACAGACGACGAAGAAAATGATCCTGACGATTATGATATAGAGGA
CTTTGAAAGAATCTTATCACCTGACATATTTTCTCCAAATAAATTAAATA
ATATGCAAAACCAACATAAGAATATAAAATTTATTAATCTTAGACACAGT
TATGATAATGATTTCAAGCGACCTGAATCAACACAAAGTGCGGTTCGAAT
GAGCTGTAGTAGTAGTAGCAGTCCAATTAGCAGTAGTTCAATGGCAACAA
ATAACATTGATAAATGTAATCCAACATATATAGATACCCAAACTGTGTCT
TTCAATGAATCTATATATTCACTCCAAAGTAGAGTGCCTCTGTTTAATGT
GAATAACAAAAACATTTTAGTCAATTATCATTTGCATTTTGCACCCCAAG
ATTCATTTATCAATTCTAGCTACGTAACATCTAATTGTGCTGAAAATGGA
ATTATTGAACAAAAATTTAAGCAAATTAGCAACGATAAACACTCATCAAG
CTCCAATTTGATAGATTCAGGAAAATATAGTGGAACTACTGTGGATAGTA
TTCTTCAACATATAACTGAACAAAAACCTTTATTTCTGCGAGAAACATCA
ACTAACAATCAGACTCTAAAGCCAGATTCTATTTTATGGCGACAGATAAA
ATTTTTGATGAATTATTATTATACCGGATGGAGATTACTGAAAATGTTTA
AACAATTTCGAAAAAAAGTCAAAACGGAACCATATTCGACAGATGAAATA
GTATTTTACCGAACAAATAGCCAATTAAAACGTAAACAAAGTTATTCTTG
TTCAGTTTTGGAAATATTTAATTCGATAAAAGATTGTGGAAAACGTCTCG
AAAACAATAGTTCGAAAAGTGATGAACAAAAGCTATTAGTTAATAAACCT
ATTCAGAATTTTAGTTGCCAATTTCCACCTAAACATCGCGTTCCGAATCA
AAGAAATCACATTACTTCTCCTCAACGAACAAATAATCAGATTTACGATT
ATCCGGGAATTGGAAGAATTGGTATGCCTAAACCAACTAGACTGAGCAGT
TCAGCATTTTTAACTTCGAATCTACATTCGCATTTATCATTTTTACAAAG
TTCTCTAACAAATCATCATTCAGATATCACCAGCAATCGCTTATATGATG
GTGATCAAATTAGCCAATCACCCAGGTCTTTGTGCAGTCTAAATTGCCAG
AAAAATCAATCAAAAAATTCTGATGCCACTCATACCCTTAAGCCATGCTT
GTTTTTTTCCATTCGGTTTTTATCACATAAATTTTTTATATACGGTATTT
GACATCACCTTTTGGCACTATTGTTGCTTTTTTAGAAAACGGACTCAAAA
TTTGAAATCATTTCTAAAGCTACGAAATCACATGAACACCATAAATTCAA
ATTGATCGAATTCTCAACAAAGTCAGTGTCACCTTAGTTCAACGAACTTT
GGATCATAACTAAACCATTTCTAGTAATCGCCACTTAAAGAAATTCATTT
GAGAAAGGAAGACGATAGTGATGAGTCAAAAGAAGTTATTTGCAGACCAA
AGTCTAATAAAATTGCTACAGATCATTTCAAAAGAAAATCTGTTAGTTTC
GATTGTCCTGGTTTATCGTCAGAACATATTATGCAAGAAATGAAAGAAAT
ACCTAAGAAATGGGGATTGTTGGGATCTGAGTCGAGATTAATTGGTGATA
TGATGGCTGTTTTTGAATGTGGTCGTAGAGATAGTCTTGTTGGAGCTTCA
GGCTTATCAGAGAACTGTTCAGGAAATGATGATGCAACGAGATTCAGAGG
TATGGTTAATGAAGTGCTAAGAGCTGTGAAACAAATATCAGATCGTTTGT
CTGTTGGAACAATGAATGGAATCGAAAAGCCATGCTCATTGGAAATGAAT
TCTTCATTTCAATCTCAATTAGCAATACTATGTGAGATGGTCAGTAATGG
AGATGGATTGGAATCTCCTTTAGCTACTGCAATTGATAATCTTATCACTT
TAATGTCAGATGGACTGGTTGTAACTTCATTTTCAAATAAAAAAGTTCGC
ATTTCTTCATCATCTGCAAATAAAGCTGCAATGCGTTTATGGTTACTTGT
TGAAGAAACTTGCCGAAACCCTGGTAGTCAAAATACGTCTCGTAAAGCTG
AACTTGCTCAGTTATTACGAGAAGTAAGACAAATCATCACGAATATATCC
TCAACAAAAGACCATCAATTGCGACTGAGGGCTTTCATTGCCAATGCTTT
AAAATCTGGTTTGTTACATGTTTGGATTAGGTCTGTTGTTGCTAATGAAA
CTGCCTTACAACGCTTTTATTGTCCTGAAGCATTTCTTCGTCAAGCCAGA
AGTGGTCAAAGTGACGCACTTGCAGCATTAACAGTCAGTCTTAAAGCTTT
GACTTCTACTCAAAATAGTCAGACTCCACTTTATAACAACCAATCAGCAC
CGATAGCTAATAAACTTGGTCTTCCTCCACCAGTAAGAATCTCACGAAGG
TTTTTTTCTAGTCCAACCGCAGAAATATCGAACAAAGTTTCTCCTAATCA
TTCACAAAAGCCTTTATATTCTGAAGAAAATACTGAAAGTGAATCCTGTG
CCGATGAATCTCGATCTTCCCAAAGTGAATGTCCTCCGAATCGCGACATC
AGTAACAATAACAACATATTTTCAAGAGCTACTCATCCCCGGACAGTTAA
AAAGTCTAATTCCTCTCAGTTGCACTCTACTACTTCAGATTCCTCTCGTA
ATTTTCCTTTTCGCAGTCCTGCTAAATCTCTAACAAATCAAAATTTCAAC
AGTCCATTAAAACCGAGCGTGGTATCGATATCAATGAAAACCATCCGAAC
TCAGCCGATAGCTTCTCGAATAAAACCATCAAAAACTGACAAATCCTAAT
ATATAGAGAAAAATGAATCGGTATCAACCGAATCTTATTGCATTTTATTT
TCAATTTTGTCATCAGCTTTTAATTTACTTTTACTATTTGACAGTCGCAT
AAAGCATTTTTTTTCTAATATTTGTAGGATGACCCTTACATATACATTTT
TTTAATTTCTGAGACATTTTGTTCTTTTAGAATTGTATTTGAAAATGTTT
TTGAAGATATAAGAAAAACAAATTGATTTTATTGTATAAAACTTATACAT
ATACAAATGACATTTTAAATTGATTTCTGTGTTAAGCAGGAATTTGTTGT
TTATTGTTGGTGGAAAAAAAACACAAACTAAATTTTTTAACTCGTTCATT
TTCGTATGTGTTTTGTCGAATTGTGCGAATGTGCAATAATCGTGTTTTCC
ATTGCATGGGGAGAAATGAAGATTTTTCATTTTGATGTTTTGCAGTGGAT
ATATCTTTTTATTGAATAAAAAATAGTGTCTTTCAAATATATTCATGTTT
TATGCTGCCTATATTTTCACTACTGTATTTGTGTGTGTGTGTGTGTTTGA
GTGTGAGCGCGCGGTTTGATTTTTAGGATATTTAACTTGTGGAAAATATT
TTCAAAGTGCACAAATTTGAAATGATGTGATTTTGCATGTAGTTGTGATA
CATTAAGATGTTTCTGATAATTTTCTTTTCAAATTAGAGTTAGAACAGTT
ATAAATTCTAGATTCTAGGTGGTCGTATGACAGCAATTCTTTTTTTCTCA
AATTATTAATATGGTCACGAGTGGGAATCTGTATTTGCGTTTTATGGTTA
TTGTATATTAGATTCTAAATTTTTAATGAAAAATAAATGATTATTTGAG
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protein sequence of SMED30012426-orf-1

>SMED30012426-orf-1 ID=SMED30012426-orf-1|Name=SMED30012426-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=115bp
MNIFERHYFLFNKKIYPLQNIKMKNLHFSPCNGKHDYCTFAQFDKTHTKM
NELKNLVCVFFPPTINNKFLLNTEINLKCHLYMYKFYTIKSICFSYIFKN
IFKYNSKRTKCLRN*
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protein sequence of SMED30012426-orf-2

>SMED30012426-orf-2 ID=SMED30012426-orf-2|Name=SMED30012426-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=484bp
KSYMMDILVESIEYYYDEDFSVPFDIGITEENNNTNLNLDYQCENILKYV
DITDDEENDPDDYDIEDFERILSPDIFSPNKLNNMQNQHKNIKFINLRHS
YDNDFKRPESTQSAVRMSCSSSSSPISSSSMATNNIDKCNPTYIDTQTVS
FNESIYSLQSRVPLFNVNNKNILVNYHLHFAPQDSFINSSYVTSNCAENG
IIEQKFKQISNDKHSSSSNLIDSGKYSGTTVDSILQHITEQKPLFLRETS
TNNQTLKPDSILWRQIKFLMNYYYTGWRLLKMFKQFRKKVKTEPYSTDEI
VFYRTNSQLKRKQSYSCSVLEIFNSIKDCGKRLENNSSKSDEQKLLVNKP
IQNFSCQFPPKHRVPNQRNHITSPQRTNNQIYDYPGIGRIGMPKPTRLSS
SAFLTSNLHSHLSFLQSSLTNHHSDITSNRLYDGDQISQSPRSLCSLNCQ
KNQSKNSDATHTLKPCLFFSIRFLSHKFFIYGI*
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protein sequence of SMED30012426-orf-3

>SMED30012426-orf-3 ID=SMED30012426-orf-3|Name=SMED30012426-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=423bp
MQEMKEIPKKWGLLGSESRLIGDMMAVFECGRRDSLVGASGLSENCSGND
DATRFRGMVNEVLRAVKQISDRLSVGTMNGIEKPCSLEMNSSFQSQLAIL
CEMVSNGDGLESPLATAIDNLITLMSDGLVVTSFSNKKVRISSSSANKAA
MRLWLLVEETCRNPGSQNTSRKAELAQLLREVRQIITNISSTKDHQLRLR
AFIANALKSGLLHVWIRSVVANETALQRFYCPEAFLRQARSGQSDALAAL
TVSLKALTSTQNSQTPLYNNQSAPIANKLGLPPPVRISRRFFSSPTAEIS
NKVSPNHSQKPLYSEENTESESCADESRSSQSECPPNRDISNNNNIFSRA
THPRTVKKSNSSQLHSTTSDSSRNFPFRSPAKSLTNQNFNSPLKPSVVSI
SMKTIRTQPIASRIKPSKTDKS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR004012Run_dom