60S ribosomal protein L37a

Overview


Name	60S ribosomal protein L37a
Smed ID	SMED30012421
Length (bp)	446

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of 60S ribosomal protein L37a (SMED30012421) t-SNE clustered cells

Violin plots show distribution of expression levels for 60S ribosomal protein L37a (SMED30012421) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 60S ribosomal protein L37a (SMED30012421) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 60S ribosomal protein L37a (SMED30012421) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30012421

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 19

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
X1 cell	SMED30012421	SMESG000078736.1	SmedASXL_051908	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
pharynx	SMED30012421	SMESG000078736.1	dd_Smed_v4_140_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30012421	SMESG000078736.1	dd_Smed_v4_140_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
gut	SMED30012421	SMESG000078736.1	dd_Smed_v4_140_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30012421	SMESG000078736.1	dd_Smed_v4_140_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30012421	SMESG000078736.1	dd_Smed_v4_140_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30012421	SMESG000078736.1	dd_Smed_v4_140_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30012421	SMESG000078736.1	dd_Smed_v4_140_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30012421	SMESG000078736.1	dd_Smed_v4_140_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
non-ciliated neuron	SMED30012421	SMESG000078736.1	dd_Smed_v4_140_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30012421	SMESG000078736.1	Contig21767	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30012421	SMESG000078736.1	Contig21767	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30012421	SMESG000078736.1	Contig52012	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30012421	SMESG000078736.1	Contig52012	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30012421	SMESG000079772.1 SMESG000078736.1 SMESG000029693.1	Contig11241	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30012421	SMESG000079772.1 SMESG000078736.1 SMESG000029693.1	Contig11241	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
head region	SMED30012421	SMESG000078736.1	OX_Smed_1.0.09315	ox_Smed_v2	PMID:24238224 Kao et al., 2013
gamma neoblast	SMED30012421	SMESG000078736.1	dd_Smed_v6_140_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018
neoblast	SMED30012421	SMESG000078736.1	dd_Smed_v6_140_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of 60S ribosomal protein L37a vs. Ensembl Human
Match: RPL37A (ribosomal protein L37a [Source:HGNC Symbol;Acc:HGNC:10348])

HSP 1 Score: 99.3673 bits (246), Expect = 1.943e-27
Identity = 47/63 (74.60%), Postives = 52/63 (82.54%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGIVGKYGTRYG+S+RK +KKIEI  HAKY CSFCGK  +KR+ VGIWHC  C K 
Sbjct:    1 MAKRTKKVGIVGKYGTRYGASLRKMVKKIEISQHAKYTCSFCGKTKMKRRAVGIWHCGSCMKT 63

[Close]

BLAST of 60S ribosomal protein L37a vs. Ensembl Human
Match: RPL37A (ribosomal protein L37a [Source:HGNC Symbol;Acc:HGNC:10348])

HSP 1 Score: 98.2117 bits (243), Expect = 3.295e-27
Identity = 47/63 (74.60%), Postives = 52/63 (82.54%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGIVGKYGTRYG+S+RK +KKIEI  HAKY CSFCGK  +KR+ VGIWHC  C K 
Sbjct:    1 MAKRTKKVGIVGKYGTRYGASLRKMVKKIEISQHAKYTCSFCGKTKMKRRAVGIWHCGSCMKT 63

[Close]

BLAST of 60S ribosomal protein L37a vs. Ensembl Human
Match: RPL37A (ribosomal protein L37a [Source:HGNC Symbol;Acc:HGNC:10348])

HSP 1 Score: 97.4413 bits (241), Expect = 4.644e-27
Identity = 47/63 (74.60%), Postives = 52/63 (82.54%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGIVGKYGTRYG+S+RK +KKIEI  HAKY CSFCGK  +KR+ VGIWHC  C K 
Sbjct:    1 MAKRTKKVGIVGKYGTRYGASLRKMVKKIEISQHAKYTCSFCGKTKMKRRAVGIWHCGSCMKT 63

[Close]

BLAST of 60S ribosomal protein L37a vs. Ensembl Human
Match: RPL37A (ribosomal protein L37a [Source:HGNC Symbol;Acc:HGNC:10348])

HSP 1 Score: 77.7962 bits (190), Expect = 2.172e-19
Identity = 37/44 (84.09%), Postives = 40/44 (90.91%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGK 238
            MAKRTKKVGIVGKYGTRYG+S+RK +KKIEI  HAKY CSFCGK
Sbjct:    1 MAKRTKKVGIVGKYGTRYGASLRKMVKKIEISQHAKYTCSFCGK 44

[Close]

BLAST of 60S ribosomal protein L37a vs. Ensembl Human
Match: RPL37A (ribosomal protein L37a [Source:HGNC Symbol;Acc:HGNC:10348])

HSP 1 Score: 57.7658 bits (138), Expect = 1.456e-11
Identity = 25/38 (65.79%), Postives = 28/38 (73.68%), Query Frame = 2

Query:  182 IKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            +KKIEI  HAKY CSFCGK  +KR+ VGIWHC  C K 
Sbjct:    2 VKKIEISQHAKYTCSFCGKTKMKRRAVGIWHCGSCMKT 39

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BLAST of 60S ribosomal protein L37a vs. Ensembl Celegans
Match: rpl-43 (60S ribosomal protein L37a [Source:UniProtKB/Swiss-Prot;Acc:Q9U2A8])

HSP 1 Score: 95.1301 bits (235), Expect = 2.374e-26
Identity = 44/63 (69.84%), Postives = 53/63 (84.13%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGIVGKYGTRYG+S+RK  KK+E+  H++Y CSFCGK+A+KRK  GIW+C  C KV
Sbjct:    1 MAKRTKKVGIVGKYGTRYGASLRKMAKKLEVAQHSRYTCSFCGKEAMKRKATGIWNCAKCHKV 63

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Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGIVGKYGTRYG+S+RK  KK+E+  H++Y CSFCGK+A+KRK  GIW+C  C KV
Sbjct:    1 MAKRTKKVGIVGKYGTRYGASLRKMAKKLEVAQHSRYTCSFCGKEAMKRKATGIWNCAKCHKV 63

[Close]

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGIVGKYGTRYG+S+RK  KK+E+  H++Y CSFCGK+A+KRK  GIW+C  C KV
Sbjct:    1 MAKRTKKVGIVGKYGTRYGASLRKMAKKLEVAQHSRYTCSFCGKEAMKRKATGIWNCAKCHKV 63

[Close]

BLAST of 60S ribosomal protein L37a vs. Ensembl Fly
Match: RpL37A (gene:FBgn0261608 transcript:FBtr0079017)

HSP 1 Score: 96.6709 bits (239), Expect = 8.767e-27
Identity = 45/63 (71.43%), Postives = 53/63 (84.13%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGIVGKYGTRYG+S+RK +KK+EI  H+KY CSFCGK ++KR  VGIW CK CK+ 
Sbjct:    1 MAKRTKKVGIVGKYGTRYGASLRKMVKKMEITQHSKYTCSFCGKDSMKRAVVGIWSCKRCKRT 63

[Close]

BLAST of 60S ribosomal protein L37a vs. Ensembl Fly
Match: RpL37A (gene:FBgn0261608 transcript:FBtr0302570)

HSP 1 Score: 96.6709 bits (239), Expect = 8.767e-27
Identity = 45/63 (71.43%), Postives = 53/63 (84.13%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGIVGKYGTRYG+S+RK +KK+EI  H+KY CSFCGK ++KR  VGIW CK CK+ 
Sbjct:    1 MAKRTKKVGIVGKYGTRYGASLRKMVKKMEITQHSKYTCSFCGKDSMKRAVVGIWSCKRCKRT 63

[Close]

BLAST of 60S ribosomal protein L37a vs. Ensembl Fly
Match: RpL37A (gene:FBgn0261608 transcript:FBtr0079016)

HSP 1 Score: 96.6709 bits (239), Expect = 8.767e-27
Identity = 45/63 (71.43%), Postives = 53/63 (84.13%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGIVGKYGTRYG+S+RK +KK+EI  H+KY CSFCGK ++KR  VGIW CK CK+ 
Sbjct:    1 MAKRTKKVGIVGKYGTRYGASLRKMVKKMEITQHSKYTCSFCGKDSMKRAVVGIWSCKRCKRT 63

[Close]

BLAST of 60S ribosomal protein L37a vs. Ensembl Zebrafish
Match: zgc:171772 (zgc:171772 [Source:ZFIN;Acc:ZDB-GENE-070928-31])

HSP 1 Score: 99.7525 bits (247), Expect = 6.938e-28
Identity = 48/63 (76.19%), Postives = 52/63 (82.54%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGIVGKYGTRYG+S+RK +KKIEI  HAKY CSFCGK  +KRK VGIWHC  C K 
Sbjct:    1 MAKRTKKVGIVGKYGTRYGASLRKMVKKIEISQHAKYTCSFCGKTKMKRKAVGIWHCGSCMKT 63

[Close]

BLAST of 60S ribosomal protein L37a vs. Ensembl Xenopus
Match: abhd3 (abhydrolase domain containing 3 [Source:Xenbase;Acc:XB-GENE-984642])

HSP 1 Score: 100.908 bits (250), Expect = 4.274e-28
Identity = 48/63 (76.19%), Postives = 53/63 (84.13%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGIVGKYGTRYG+S+RK +KKIEI  HAKY CSFCGK  +KRK VGIWHC  C K+
Sbjct:    1 MAKRTKKVGIVGKYGTRYGASLRKMVKKIEISQHAKYTCSFCGKTKMKRKAVGIWHCGSCMKI 63

[Close]

BLAST of 60S ribosomal protein L37a vs. Ensembl Xenopus
Match: gtf3c5 (general transcription factor IIIC subunit 5 [Source:Xenbase;Acc:XB-GENE-1012275])

HSP 1 Score: 83.5741 bits (205), Expect = 1.635e-21
Identity = 41/56 (73.21%), Postives = 46/56 (82.14%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWH 274
            MAKRTKKVGIVGKY T YG+S+RK +KKIEI  HAKY CSFCGK  +KRK +GI H
Sbjct:    1 MAKRTKKVGIVGKYWTWYGASLRKMVKKIEINQHAKYTCSFCGKTKMKRKAIGILH 56

[Close]

BLAST of 60S ribosomal protein L37a vs. Ensembl Mouse
Match: Rpl37a (ribosomal protein L37a [Source:MGI Symbol;Acc:MGI:98068])

HSP 1 Score: 99.3673 bits (246), Expect = 1.334e-27
Identity = 47/63 (74.60%), Postives = 52/63 (82.54%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGIVGKYGTRYG+S+RK +KKIEI  HAKY CSFCGK  +KR+ VGIWHC  C K 
Sbjct:    1 MAKRTKKVGIVGKYGTRYGASLRKMVKKIEISQHAKYTCSFCGKTKMKRRAVGIWHCGSCMKT 63

[Close]

BLAST of 60S ribosomal protein L37a vs. Ensembl Mouse
Match: Rpl37a (ribosomal protein L37a [Source:MGI Symbol;Acc:MGI:98068])

HSP 1 Score: 48.9062 bits (115), Expect = 2.262e-8
Identity = 21/33 (63.64%), Postives = 23/33 (69.70%), Query Frame = 2

Query:  197 IQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            I  HAKY CSFCGK  +KR+ VGIWHC  C K 
Sbjct:    2 ISQHAKYTCSFCGKTKMKRRAVGIWHCGSCMKT 34

[Close]

BLAST of 60S ribosomal protein L37a vs. UniProt/SwissProt
Match: sp|Q751L1|RL43_ASHGO (60S ribosomal protein L43 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=RPL43 PE=3 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 9.892e-28
Identity = 45/63 (71.43%), Postives = 53/63 (84.13%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGI GKYG RYGSS+R+ +KK+E+Q HA+YDCSFCGKKA+KR   GIW+C  CKK 
Sbjct:    1 MAKRTKKVGITGKYGVRYGSSLRRQVKKLEVQQHARYDCSFCGKKAVKRGAAGIWNCSSCKKT 63

[Close]

BLAST of 60S ribosomal protein L37a vs. UniProt/SwissProt
Match: sp|P32046|RL37A_CHICK (60S ribosomal protein L37a OS=Gallus gallus OX=9031 GN=RPL37A PE=3 SV=2)

HSP 1 Score: 99.7525 bits (247), Expect = 4.960e-27
Identity = 48/63 (76.19%), Postives = 52/63 (82.54%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGIVGKYGTRYG+S+RK +KKIEI  HAKY CSFCGK  +KRK VGIWHC  C K 
Sbjct:    1 MAKRTKKVGIVGKYGTRYGASLRKMVKKIEISQHAKYTCSFCGKTKMKRKAVGIWHCGSCMKT 63

[Close]

BLAST of 60S ribosomal protein L37a vs. UniProt/SwissProt
Match: sp|O61462|RL37A_CRYST (60S ribosomal protein L37a OS=Cryptochiton stelleri OX=6655 GN=RPL37A PE=3 SV=3)

HSP 1 Score: 99.7525 bits (247), Expect = 5.840e-27
Identity = 46/63 (73.02%), Postives = 54/63 (85.71%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGIVGKYGTRYG+S+RK +KK+EI  H+KY C FCGK A+KR+ VGIW CK C+KV
Sbjct:    1 MAKRTKKVGIVGKYGTRYGASLRKTVKKMEITQHSKYTCQFCGKDAMKRQAVGIWGCKSCRKV 63

[Close]

BLAST of 60S ribosomal protein L37a vs. UniProt/SwissProt
Match: sp|Q7SZB4|RL37A_XENLA (60S ribosomal protein L37a OS=Xenopus laevis OX=8355 GN=rpl37a PE=3 SV=3)

HSP 1 Score: 99.7525 bits (247), Expect = 6.235e-27
Identity = 47/63 (74.60%), Postives = 53/63 (84.13%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGIVGKYGTR+G+S+RK +KKIEI  HAKY CSFCGK  +KRK VGIWHC  C K+
Sbjct:    1 MAKRTKKVGIVGKYGTRFGASLRKMVKKIEISQHAKYTCSFCGKTKMKRKAVGIWHCGSCMKI 63

[Close]

BLAST of 60S ribosomal protein L37a vs. UniProt/SwissProt
Match: sp|Q90YT0|RL37A_ICTPU (60S ribosomal protein L37a OS=Ictalurus punctatus OX=7998 GN=rpl37a PE=3 SV=3)

HSP 1 Score: 99.3673 bits (246), Expect = 9.029e-27
Identity = 47/63 (74.60%), Postives = 52/63 (82.54%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGIVGKYGTRYG+S+RK +KKIEI  HAKY CSFCGK  +KR+ VGIWHC  C K 
Sbjct:    1 MAKRTKKVGIVGKYGTRYGASLRKMVKKIEISQHAKYTCSFCGKTKMKRRAVGIWHCGSCMKT 63

[Close]

BLAST of 60S ribosomal protein L37a vs. TrEMBL
Match: A7RI41 (Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g226223 PE=3 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 4.270e-27
Identity = 50/63 (79.37%), Postives = 55/63 (87.30%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVG+ GKYGTRYG+S+RK IKKIEIQ H+KY C FCGK+ LKRKCVGIWHCKGC K 
Sbjct:    1 MAKRTKKVGVTGKYGTRYGASLRKMIKKIEIQQHSKYTCVFCGKETLKRKCVGIWHCKGCGKT 63

[Close]

BLAST of 60S ribosomal protein L37a vs. TrEMBL
Match: A0A267F9T5 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig029688g2 PE=3 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 8.733e-27
Identity = 48/64 (75.00%), Postives = 56/64 (87.50%), Query Frame = 2

Query:  104 SMAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
             MAKRTKKVGIVGKY TRYG+S+RK +KKIEIQ H+KY CSFCGK AL+R+ VGIWHC+GC+K 
Sbjct:    1 EMAKRTKKVGIVGKYATRYGASLRKTVKKIEIQQHSKYTCSFCGKDALRRQAVGIWHCRGCRKT 64

[Close]

BLAST of 60S ribosomal protein L37a vs. TrEMBL
Match: A0A267GFZ1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig026016g1 PE=3 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 1.010e-26
Identity = 48/63 (76.19%), Postives = 56/63 (88.89%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGIVGKY TRYG+S+RK +KKIEIQ H+KY CSFCGK AL+R+ VGIWHC+GC+K 
Sbjct:    1 MAKRTKKVGIVGKYATRYGASLRKTVKKIEIQQHSKYTCSFCGKDALRRQAVGIWHCRGCRKT 63

[Close]

BLAST of 60S ribosomal protein L37a vs. TrEMBL
Match: A0A0L0TB51 (60S ribosomal protein L37a OS=Allomyces macrogynus (strain ATCC 38327) OX=578462 GN=AMAG_05221 PE=3 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 1.313e-26
Identity = 49/63 (77.78%), Postives = 55/63 (87.30%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGIVGKYGTRYG+++RK IKK+EI  HAKY CSFCGK ++KR  VGIWHCKGCKK 
Sbjct:    1 MAKRTKKVGIVGKYGTRYGATLRKQIKKMEITQHAKYTCSFCGKDSVKRTAVGIWHCKGCKKT 63

[Close]

BLAST of 60S ribosomal protein L37a vs. TrEMBL
Match: A0A2B4SWG0 (60S ribosomal protein L37a OS=Stylophora pistillata OX=50429 GN=RPL37A PE=3 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 1.432e-26
Identity = 48/62 (77.42%), Postives = 56/62 (90.32%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKK 292
            MAKRTKKVG+VGKYGTRYG+S+RK +KK+EI  H+KY C FCGK+ALKR+CVGIWHCKGC K
Sbjct:    1 MAKRTKKVGVVGKYGTRYGASLRKMVKKMEITQHSKYTCVFCGKEALKRQCVGIWHCKGCGK 62

[Close]

BLAST of 60S ribosomal protein L37a vs. Ensembl Cavefish
Match: zgc:171772 (ribosomal protein L37a [Source:NCBI gene;Acc:103037274])

HSP 1 Score: 97.8265 bits (242), Expect = 3.744e-27
Identity = 46/63 (73.02%), Postives = 51/63 (80.95%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGIVGKYGTRYG+S+RK +KKIEI  HAKY C FCGK  +KR+ VGIWHC  C K 
Sbjct:    1 MAKRTKKVGIVGKYGTRYGASLRKMVKKIEISQHAKYTCYFCGKTKMKRRAVGIWHCTSCMKT 63

[Close]

BLAST of 60S ribosomal protein L37a vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000001.1 (pep scaffold:Pmarinus_7.0:GL476426:6020:9121:-1 gene:ENSPMAG00000000001.1 transcript:ENSPMAT00000000001.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 99.3673 bits (246), Expect = 2.034e-28
Identity = 47/63 (74.60%), Postives = 52/63 (82.54%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGIVGKYGTRYG+S+RK +KKIEI  HAKY CSFCGK  +KR+ VGIWHC  C K 
Sbjct:    1 MAKRTKKVGIVGKYGTRYGASLRKMVKKIEISQHAKYTCSFCGKTKMKRRAVGIWHCGSCMKT 63

[Close]

BLAST of 60S ribosomal protein L37a vs. Ensembl Yeast
Match: RPL43B (Ribosomal 60S subunit protein L43B; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43B has a paralog, RPL43A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000003855])

HSP 1 Score: 97.4413 bits (241), Expect = 6.237e-28
Identity = 45/63 (71.43%), Postives = 51/63 (80.95%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGI GKYG RYGSS+R+ +KK+EIQ HA+YDCSFCGKK +KR   GIW C  CKK 
Sbjct:    1 MAKRTKKVGITGKYGVRYGSSLRRQVKKLEIQQHARYDCSFCGKKTVKRGAAGIWTCSCCKKT 63

[Close]

BLAST of 60S ribosomal protein L37a vs. Ensembl Yeast
Match: RPL43A (Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication [Source:SGD;Acc:S000006247])

HSP 1 Score: 97.4413 bits (241), Expect = 6.237e-28
Identity = 45/63 (71.43%), Postives = 51/63 (80.95%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVGI GKYG RYGSS+R+ +KK+EIQ HA+YDCSFCGKK +KR   GIW C  CKK 
Sbjct:    1 MAKRTKKVGITGKYGVRYGSSLRRQVKKLEIQQHARYDCSFCGKKTVKRGAAGIWTCSCCKKT 63

[Close]

BLAST of 60S ribosomal protein L37a vs. Ensembl Yeast
Match: OPI11 (Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; largely overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO [Source:SGD;Acc:S000006248])

HSP 1 Score: 46.595 bits (109), Expect = 6.773e-8
Identity = 25/74 (33.78%), Postives = 41/74 (55.41%), Query Frame = -2

Query:   77 NNFLATFAVPNSDTLSF*CFLSTERTVIFSVRLNFNFLNIFSD*RTVSCSIFTDDANLLCSFGH*YYLNFVFEY 298
            +  L T   PN+ + S    L+TERT++ SV L+F  L++ S   T++ ++ T D NLL SF   +    + +Y
Sbjct:   40 DGLLTTGTSPNTSSTSLDGLLTTERTIVSSVLLDFQLLDLSSQRGTITDTVLTSDTNLLSSFSPVFKKEKISKY 113

[Close]

BLAST of 60S ribosomal protein L37a vs. Ensembl Nematostella
Match: EDO48962 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RI41])

HSP 1 Score: 106.301 bits (264), Expect = 5.905e-31
Identity = 50/63 (79.37%), Postives = 55/63 (87.30%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            MAKRTKKVG+ GKYGTRYG+S+RK IKKIEIQ H+KY C FCGK+ LKRKCVGIWHCKGC K 
Sbjct:    1 MAKRTKKVGVTGKYGTRYGASLRKMIKKIEIQQHSKYTCVFCGKETLKRKCVGIWHCKGCGKT 63

[Close]

BLAST of 60S ribosomal protein L37a vs. Ensembl Medaka
Match: zgc:171772 (ribosomal protein L37a [Source:NCBI gene;Acc:101161674])

HSP 1 Score: 98.2117 bits (243), Expect = 2.326e-27
Identity = 47/62 (75.81%), Postives = 52/62 (83.87%), Query Frame = 2

Query:  110 AKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKV 295
            AKRTKKVGIVGKYGTRYG+S+RK +KKIEI  HAKY CSFCGK  +KR+ VGIWHC  CKK 
Sbjct:    1 AKRTKKVGIVGKYGTRYGASLRKMVKKIEISQHAKYTCSFCGKTKMKRRAVGIWHCGSCKKT 62

[Close]

BLAST of 60S ribosomal protein L37a vs. Planmine SMEST
Match: SMESG000078736.1 (SMESG000078736.1)

HSP 1 Score: 127.487 bits (319), Expect = 6.069e-39
Identity = 90/90 (100.00%), Postives = 90/90 (100.00%), Query Frame = 2

Query:  107 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKVXXXXXXXXXXXXXXXXXXXXXXXNEGR 376
            MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKVIAGGAYTHTTTAAVTARAHIRRVNEGR
Sbjct:    1 MAKRTKKVGIVGKYGTRYGSSIRKNIKKIEIQSHAKYDCSFCGKKALKRKCVGIWHCKGCKKVIAGGAYTHTTTAAVTARAHIRRVNEGR 90

The following BLAST results are available for this feature:

BLAST of 60S ribosomal protein L37a vs. Ensembl Human
BLAST of 60S ribosomal protein L37a vs. Ensembl Celegans
BLAST of 60S ribosomal protein L37a vs. Ensembl Fly
BLAST of 60S ribosomal protein L37a vs. Ensembl Zebrafish
BLAST of 60S ribosomal protein L37a vs. Ensembl Xenopus
BLAST of 60S ribosomal protein L37a vs. Ensembl Mouse
BLAST of 60S ribosomal protein L37a vs. UniProt/SwissProt
BLAST of 60S ribosomal protein L37a vs. TrEMBL
BLAST of 60S ribosomal protein L37a vs. Ensembl Cavefish
BLAST of 60S ribosomal protein L37a vs. Ensembl Sea Lamprey
BLAST of 60S ribosomal protein L37a vs. Ensembl Yeast
BLAST of 60S ribosomal protein L37a vs. Ensembl Nematostella
BLAST of 60S ribosomal protein L37a vs. Ensembl Medaka
BLAST of 60S ribosomal protein L37a vs. Planmine SMEST

BLAST of 60S ribosomal protein L37a vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
RPL37A	1.943e-27	74.60	ribosomal protein L37a [Source:HGNC Symbol;Acc:HGN...	[more]
RPL37A	3.295e-27	74.60	ribosomal protein L37a [Source:HGNC Symbol;Acc:HGN...	[more]
RPL37A	4.644e-27	74.60	ribosomal protein L37a [Source:HGNC Symbol;Acc:HGN...	[more]
RPL37A	2.172e-19	84.09	ribosomal protein L37a [Source:HGNC Symbol;Acc:HGN...	[more]
RPL37A	1.456e-11	65.79	ribosomal protein L37a [Source:HGNC Symbol;Acc:HGN...	[more]

BLAST of 60S ribosomal protein L37a vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3


Match Name	E-value	Identity	Description
rpl-43	2.374e-26	69.84	60S ribosomal protein L37a [Source:UniProtKB/Swis...	[more]
rpl-43	2.374e-26	69.84	60S ribosomal protein L37a [Source:UniProtKB/Swis...	[more]
rpl-43	2.374e-26	69.84	60S ribosomal protein L37a [Source:UniProtKB/Swis...	[more]

BLAST of 60S ribosomal protein L37a vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3


Match Name	E-value	Identity	Description
RpL37A	8.767e-27	71.43	gene:FBgn0261608 transcript:FBtr0079017	[more]
RpL37A	8.767e-27	71.43	gene:FBgn0261608 transcript:FBtr0302570	[more]
RpL37A	8.767e-27	71.43	gene:FBgn0261608 transcript:FBtr0079016	[more]

BLAST of 60S ribosomal protein L37a vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
zgc:171772	6.938e-28	76.19	zgc:171772 [Source:ZFIN;Acc:ZDB-GENE-070928-31]	[more]

BLAST of 60S ribosomal protein L37a vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2


Match Name	E-value	Identity	Description
abhd3	4.274e-28	76.19	abhydrolase domain containing 3 [Source:Xenbase;Ac...	[more]
gtf3c5	1.635e-21	73.21	general transcription factor IIIC subunit 5 [Sourc...	[more]

BLAST of 60S ribosomal protein L37a vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2


Match Name	E-value	Identity	Description
Rpl37a	1.334e-27	74.60	ribosomal protein L37a [Source:MGI Symbol;Acc:MGI:...	[more]
Rpl37a	2.262e-8	63.64	ribosomal protein L37a [Source:MGI Symbol;Acc:MGI:...	[more]

BLAST of 60S ribosomal protein L37a vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q751L1\|RL43_ASHGO	9.892e-28	71.43	60S ribosomal protein L43 OS=Ashbya gossypii (stra...	[more]
sp\|P32046\|RL37A_CHICK	4.960e-27	76.19	60S ribosomal protein L37a OS=Gallus gallus OX=903...	[more]
sp\|O61462\|RL37A_CRYST	5.840e-27	73.02	60S ribosomal protein L37a OS=Cryptochiton steller...	[more]
sp\|Q7SZB4\|RL37A_XENLA	6.235e-27	74.60	60S ribosomal protein L37a OS=Xenopus laevis OX=83...	[more]
sp\|Q90YT0\|RL37A_ICTPU	9.029e-27	74.60	60S ribosomal protein L37a OS=Ictalurus punctatus ...	[more]

BLAST of 60S ribosomal protein L37a vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A7RI41	4.270e-27	79.37	Predicted protein OS=Nematostella vectensis OX=453...	[more]
A0A267F9T5	8.733e-27	75.00	Uncharacterized protein (Fragment) OS=Macrostomum ...	[more]
A0A267GFZ1	1.010e-26	76.19	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]
A0A0L0TB51	1.313e-26	77.78	60S ribosomal protein L37a OS=Allomyces macrogynus...	[more]
A0A2B4SWG0	1.432e-26	77.42	60S ribosomal protein L37a OS=Stylophora pistillat...	[more]

BLAST of 60S ribosomal protein L37a vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
zgc:171772	3.744e-27	73.02	ribosomal protein L37a [Source:NCBI gene;Acc:10303...	[more]

BLAST of 60S ribosomal protein L37a vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSPMAT00000000001.1	2.034e-28	74.60	pep scaffold:Pmarinus_7.0:GL476426:6020:9121:-1 ge...	[more]

BLAST of 60S ribosomal protein L37a vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 3


Match Name	E-value	Identity	Description
RPL43B	6.237e-28	71.43	Ribosomal 60S subunit protein L43B; homologous to ...	[more]
RPL43A	6.237e-28	71.43	Ribosomal 60S subunit protein L43A; null mutation ...	[more]
OPI11	6.773e-8	33.78	Dubious open reading frame; unlikely to encode a f...	[more]

BLAST of 60S ribosomal protein L37a vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO48962	5.905e-31	79.37	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of 60S ribosomal protein L37a vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
zgc:171772	2.326e-27	75.81	ribosomal protein L37a [Source:NCBI gene;Acc:10116...	[more]

BLAST of 60S ribosomal protein L37a vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000078736.1	6.069e-39	100.00	SMESG000078736.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence

transcript sequence

>SMED30012421 ID=SMED30012421|Name=60S ribosomal protein L37a|organism=Schmidtea mediterranea sexual|type=transcript|length=446bp
AAAAGTTTTGGTAAAATAAAACTATTTTAGAAATATTTATTTTAATTATA
AAATTGTTGTTTAAATTTACTATTATATATTCAAATACAAAATTCAAATA
ATATCAATGGCCAAACGAACAAAGAAGGTTGGCATCGTCGGTAAATATGG
AACAAGATACGGTTCTTCAATCAGAAAAAATATTAAGAAAATTGAAATTC
AATCGCACGCTAAATATGACTGTTCTTTCTGTGGAAAGAAAGCATTAAAA
AGAAAGTGTGTCGGAATTTGGCACTGCAAAGGTTGCAAGAAAGTTATTGC
TGGAGGTGCTTATACTCACACCACCACAGCAGCAGTTACTGCTCGTGCAC
ATATTAGACGTGTTAACGAAGGCCGTTAATTTTTAAATAATAAATTTTGG
AGTAATGAAAAAAAAAAAAAAAAAANNNNATATTCCTGGAGTCTCG

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000026	gut
PLANA:0000039	gamma neoblast
PLANA:0000418	head
PLANA:0000429	neoblast
PLANA:0002032	epidermal cell
PLANA:0002109	X1 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR002674	Ribosomal_L37ae
IPR011331	Ribosomal_L37ae/L37e
IPR011332	Ribosomal_zn-bd

Vocabulary: **cellular component**
Term	Definition
GO:0005622	intracellular
GO:0005840	ribosome
GO:0005634	nucleus
GO:0005925	focal adhesion
GO:0022625	cytosolic large ribosomal subunit
GO:0030529	intracellular ribonucleoprotein complex
GO:0070062	extracellular exosome

Vocabulary: **biological process**
Term	Definition
GO:0006412	translation

Vocabulary: **molecular function**
Term	Definition
GO:0003735	structural constituent of ribosome
GO:0003723	RNA binding
GO:0046872	metal ion binding

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002674	Ribosomal protein L37ae	PFAM	PF01780	Ribosomal_L37ae	coord: 4..88 e-value: 5.4E-39 score: 132.2
IPR002674	Ribosomal protein L37ae	TIGRFAM	TIGR00280	TIGR00280	coord: 1..89 e-value: 1.8E-35 score: 119.1
IPR011331	Ribosomal protein L37ae/L37e	GENE3D	G3DSA:2.20.25.30		coord: 1..90 e-value: 5.1E-42 score: 144.0
IPR011332	Zinc-binding ribosomal protein	SUPERFAMILY	SSF57829	Zn-binding ribosomal proteins	coord: 10..80