SMED30012409

Overview
NameSMED30012409
Smed IDSMED30012409
Length (bp)458
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30012409 (SMED30012409) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30012409 (SMED30012409) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30012409 (SMED30012409) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30012409 (SMED30012409) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012409

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 17

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30012409SMESG000023005.1 Contig4394newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30012409SMESG000023005.1 Contig4394uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30012409SMESG000038746.1 Contig4394newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30012409SMESG000038746.1 Contig4394uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
Smed sexual biotypeSMED30012409SMESG000023005.1 Contig45532newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012409SMESG000023005.1 Contig45532uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012409SMESG000023005.1 Contig16227newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012409SMESG000023005.1 Contig16227uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012409SMESG000023005.1 Contig4394newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012409SMESG000023005.1 Contig4394uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012409SMESG000023005.1 Contig6038newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012409SMESG000023005.1 Contig6038uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012409SMESG000038746.1 Contig4394newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012409SMESG000038746.1 Contig4394uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30012409SMESG000023005.1 dd_Smed_v6_1571_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30012409SMESG000023005.1 dd_Smed_v6_1571_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30012409SMESG000023005.1 dd_Smed_v6_1571_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30012409 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002725:46777..47234 -2236
v31.002725:45799..46256 -2236
v31.002725:44821..45278 -2200
v31.002725:47266..47722 -730
Homology
[Close]
BLAST of SMED30012409 vs. Planmine SMEST
Match: SMESG000023005.1 (SMESG000023005.1)

HSP 1 Score: 304.679 bits (779), Expect = 2.026e-96
Identity = 149/152 (98.03%), Postives = 149/152 (98.03%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGC 457
            DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVK IINS REQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETL ITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGC
Sbjct: 1982 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKGIINSLREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLMITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGC 2133

HSP 2 Score: 304.679 bits (779), Expect = 2.026e-96
Identity = 149/152 (98.03%), Postives = 149/152 (98.03%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGC 457
            DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVK IINS REQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETL ITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGC
Sbjct: 2308 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKGIINSLREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLMITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGC 2459

HSP 3 Score: 298.516 bits (763), Expect = 2.284e-94
Identity = 146/152 (96.05%), Postives = 148/152 (97.37%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGC 457
            DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETP+ S S+  KANKSKKVQVGHPRLNEILDVECQSGC
Sbjct: 2617 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPNYSISEHNKANKSKKVQVGHPRLNEILDVECQSGC 2768

HSP 4 Score: 214.927 bits (546), Expect = 4.387e-65
Identity = 108/151 (71.52%), Postives = 126/151 (83.44%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 454
            DEFMIEENKEQNLEAS FIEDLIDEATQYDDEPVK+ I SQ ++ GKKLQVGI +++GD EVSCQTG ++ DSE QT L +  + LT T +SK FSE++   P+K+Y++KEIQCET DDS S+  KANKSKKVQVGHPRLNEILDVECQSG
Sbjct: 2145 DEFMIEENKEQNLEASQFIEDLIDEATQYDDEPVKLTIVSQTKKIGKKLQVGISKMNGDLEVSCQTGHLVIDSESQTQLEEPNDKLTTTTYSKVFSEEILIGPIKDYFDKEIQCETTDDSISEHNKANKSKKVQVGHPRLNEILDVECQSG 2295

HSP 5 Score: 213.772 bits (543), Expect = 9.896e-65
Identity = 108/152 (71.05%), Postives = 126/152 (82.89%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGC 457
            DEFMIEE KE+NLE  LF+EDLI+EATQYD+E + V I +Q E KGKKLQVGIPRLD DFEVSCQTG +I DSECQT++++ ++TLTITMHSKDFSEKLFKEP K Y EKEIQ ET + S S  +K  KSKK+QVGHPRLN+ L+ ECQSGC
Sbjct: 2780 DEFMIEERKEKNLEERLFVEDLIEEATQYDEENLNVTIGAQIETKGKKLQVGIPRLDVDFEVSCQTGHLIIDSECQTEIDNEIDTLTITMHSKDFSEKLFKEP-KNYLEKEIQFETAEVSDSQLVKTKKSKKIQVGHPRLNKSLEGECQSGC 2930

HSP 6 Score: 205.682 bits (522), Expect = 6.638e-62
Identity = 101/152 (66.45%), Postives = 127/152 (83.55%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGC 457
            DEFMI E KE+NLE SLF +DLIDE+TQ+D E + V +   +E KGKKLQVG  RL  DFEVSCQTG +I +SECQT++++ ++TLTITMHSKD+SE+LF+EP+K Y+EKEIQ ET ++S S+ +KA+KSKKVQVGHPRLNE L+VECQSGC
Sbjct: 3267 DEFMIMERKERNLEESLFFDDLIDESTQFDGENLNVTVAVPKEIKGKKLQVGSARLYDDFEVSCQTGHLIMNSECQTEIDNEIDTLTITMHSKDYSERLFREPIK-YFEKEIQFETTENSYSEHVKASKSKKVQVGHPRLNENLEVECQSGC 3417

HSP 7 Score: 204.142 bits (518), Expect = 2.865e-61
Identity = 100/152 (65.79%), Postives = 127/152 (83.55%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGC 457
            +EFMI E KE+NLE SLF +DLIDE+TQ+D E + V +   +E KGKKLQVG  RL  DFEVSCQTG +I +SECQT++++ ++TLTITMHSKD+SE+LF+EP+K Y+EKEIQ ET ++S S+ +KA+KSKKVQVGHPRLNE L+VECQSGC
Sbjct: 3591 EEFMIMERKERNLEESLFFDDLIDESTQFDGENLNVTVAVPKEIKGKKLQVGSARLYDDFEVSCQTGHLIMNSECQTEIDNEIDTLTITMHSKDYSERLFREPIK-YFEKEIQFETTENSYSEHVKASKSKKVQVGHPRLNENLEVECQSGC 3741

HSP 8 Score: 188.734 bits (478), Expect = 5.500e-56
Identity = 98/152 (64.47%), Postives = 121/152 (79.61%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGC 457
            +E MI  +KEQNLEAS  I+DLI+E+TQYDDE + V    Q+E KGKKLQVG  +L+GD EVSCQTG +I DSECQT+ +D  +T+TIT++SKDF E   ++ +KEY EKEIQ ET +DS S+ +KANKSKKVQVGHPRLNE L+VECQSGC
Sbjct: 3429 NEIMIMGSKEQNLEASRLIDDLIEESTQYDDEVIIVTNAMQKEVKGKKLQVGTSKLEGDLEVSCQTGHLIIDSECQTEFDDLSDTVTITLNSKDFGENK-EDSLKEYLEKEIQFETTEDSYSEHVKANKSKKVQVGHPRLNENLEVECQSGC 3579

HSP 9 Score: 188.734 bits (478), Expect = 5.500e-56
Identity = 98/152 (64.47%), Postives = 121/152 (79.61%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGC 457
            +E MI  +KEQNLEAS  I+DLI+E+TQYDDE + V    Q+E KGKKLQVG  +L+GD EVSCQTG +I DSECQT+ +D  +T+TIT++SKDF E   ++ +KEY EKEIQ ET +DS S+ +KANKSKKVQVGHPRLNE L+VECQSGC
Sbjct: 3753 NEIMIMGSKEQNLEASRLIDDLIEESTQYDDEVIIVTNAMQKEVKGKKLQVGTSKLEGDLEVSCQTGHLIIDSECQTEFDDLSDTVTITLNSKDFGENK-EDSLKEYLEKEIQFETTEDSYSEHVKANKSKKVQVGHPRLNENLEVECQSGC 3903

HSP 10 Score: 171.785 bits (434), Expect = 5.698e-50
Identity = 97/154 (62.99%), Postives = 119/154 (77.27%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFS--EKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGC 457
            DEFMI E KE+NLEASLF+EDLIDEATQYDD+ +   I + ++ KGKKLQVG PRLD D EVSCQTG +I DS  QTD++D  + + IT   +DFS  +KLF+E  K  ++KEIQ ET +DS S+  K++KSKKVQVGHPRLNE L+VECQSGC
Sbjct: 3915 DEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHKDVKGKKLQVGTPRLDADPEVSCQTGHLIMDSGDQTDIDDIKDKIIITAQPRDFSVEDKLFQEK-KIVFDKEIQFETTEDSYSEHGKSSKSKKVQVGHPRLNENLEVECQSGC 4067

HSP 11 Score: 168.318 bits (425), Expect = 7.002e-49
Identity = 93/152 (61.18%), Postives = 110/152 (72.37%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGC 457
            DEFMIEENKEQNLEAS FIEDLIDEATQYDDEPVK+ I S + +  K++                T  ++ DSE QT L +  + LT T +SK FSE++   P+K+Y++KEIQCET DDS S+  KANKSKKVQVGHPRLNEILDVECQSGC
Sbjct: 2471 DEFMIEENKEQNLEASQFIEDLIDEATQYDDEPVKLTIVSNK-KDWKEI----------------TRHLVIDSESQTQLEEPNDKLTTTTYSKVFSEEILIGPIKDYFDKEIQCETTDDSISEHNKANKSKKVQVGHPRLNEILDVECQSGC 2605

HSP 12 Score: 164.081 bits (414), Expect = 2.180e-47
Identity = 84/151 (55.63%), Postives = 113/151 (74.83%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 454
            +EFMI ENK +NLEA+LFIEDLI+E+TQYD+  + +     +E KGKKLQVG  RLD D EVSCQTG +I +SECQT+ ++ V+ +T  ++++DFSE+  K  + E+  KEIQ E  DD+ S   K+NKSKKVQVGH +L+E ++VECQSG
Sbjct: 4393 EEFMIMENKVRNLEANLFIEDLIEESTQYDEGNLNITTAIHKEVKGKKLQVGTSRLDSDLEVSCQTGHLITESECQTEFDEKVDEITFLLNAQDFSEEKPKRSVFEFVNKEIQFEASDDTHSISDKSNKSKKVQVGHAKLHEGVEVECQSG 4543

HSP 13 Score: 164.081 bits (414), Expect = 2.223e-47
Identity = 89/152 (58.55%), Postives = 114/152 (75.00%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGC 457
            DEFMI ENKE+NLEASL IEDLI+E+TQYDDE + V I+  +E KGKKLQVG  +LD D EVSCQTGR+I +SECQT+ N   E + ITM+S DF++ L  +  K++ EKE+QC++ D+S   + K  KS KVQ+G  R+NE  +VECQSGC
Sbjct: 4556 DEFMIMENKERNLEASLLIEDLIEESTQYDDENLNVTISVLKETKGKKLQVGCSKLDEDLEVSCQTGRLIMNSECQTEFN---EQIIITMNSPDFTD-LKIQQKKDFMEKEMQCDSLDNSLLKENKITKSMKVQMGLARINECREVECQSGC 4703

HSP 14 Score: 162.155 bits (409), Expect = 1.211e-46
Identity = 86/151 (56.95%), Postives = 111/151 (73.51%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 454
            +E MI  NKEQNLE S  I+DLI+E+TQYD E + V    Q+E KGKKLQVG P+L+GD EVSCQTG +I DSECQT+ +D  +T+ IT++SKD+ EK  + P+KEY EKE+Q E  ++S +  +K   S KVQVG PRL+E  +VECQSG
Sbjct: 4079 NEIMIMGNKEQNLEVSRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLIIDSECQTEFDDLTDTVIITLNSKDYGEK-NENPLKEYLEKEMQFEPIEESYTRLVKTYASMKVQVGFPRLDENREVECQSG 4228

HSP 15 Score: 161.77 bits (408), Expect = 1.441e-46
Identity = 88/152 (57.89%), Postives = 107/152 (70.39%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGC 457
            DEFMIEEN EQNLEASL I+DLI+E  QY DE   V + SQ+EQK KKLQVG PRLDGD EVSCQTG +I DS+CQT+ +   +T  IT+ SKDF++   K  + +  EKEIQ E  DDS     K  KSKK+QVG  +L+E  + ECQ+GC
Sbjct: 2942 DEFMIEENNEQNLEASLLIDDLIEENIQYSDESSSVTVLSQKEQKRKKLQVGNPRLDGDLEVSCQTGHLIIDSQCQTEFDWLPDTSAITLVSKDFNQVARKYSLTDLAEKEIQFELIDDSFLRDEKCKKSKKIQVGFSKLSECSEAECQTGC 3093

HSP 16 Score: 156.762 bits (395), Expect = 1.022e-44
Identity = 82/149 (55.03%), Postives = 105/149 (70.47%), Query Frame = 2 
Query:   11 MIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGC 457
            M+ +NKE++LE     +DLI+E+ QY+   V V        KGKKLQ+G PRLDG+ EVSCQTG +I DS CQTDL++  +T  IT H  DF+E+L +E +KE+ EKE+Q    DD +    +ANKSKKVQVGHPRLNE LDVECQ+GC
Sbjct: 4244 MMLDNKERSLEFGFLFDDLINESIQYN---VAV--------KGKKLQIGFPRLDGELEVSCQTGHLILDSHCQTDLDELTDTFIITNHLPDFTERLNREFLKEFAEKEVQYGDFDDYSLIHDEANKSKKVQVGHPRLNESLDVECQTGC 4381

HSP 17 Score: 137.117 bits (344), Expect = 7.415e-38
Identity = 77/148 (52.03%), Postives = 102/148 (68.92%), Query Frame = 2 
Query:   14 IEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGC 457
            I E++E+ +EASLFI+DLIDE TQYDD  + V I SQ+E KGKKLQVG PRL+ D ++SCQ G ++ DSECQT   +S ++++IT +  DFSE   +  +K++ EKEIQ E  +D +S       SK VQVG  RL E  DVECQ+ C
Sbjct: 4719 IIESRERKIEASLFIDDLIDETTQYDDLSLNVRIISQKEYKGKKLQVGSPRLELDSDMSCQVGHLVFDSECQT---ESFDSISITKYRSDFSEVPTERHLKDFVEKEIQFELLEDCSSTINNVKISKNVQVGQARLAEGRDVECQAVC 4863

HSP 18 Score: 130.568 bits (327), Expect = 1.398e-35
Identity = 71/152 (46.71%), Postives = 105/152 (69.08%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGC 457
            D+F+I + KE +LE SL  ++LI E  QYDDE VK+ ++ Q E KGK +Q G  +L  D E SCQTG +I DSECQT+ +++ E ++++++ +DFSE+   + +K + +KEIQ +   DS   ++   KSKK+QVGH RLN+  +VECQSGC
Sbjct: 3105 DKFIIIDFKEDSLETSLRTDNLIHEEIQYDDEDVKMRLSPQAEHKGKIIQAGFSKLGFDLEASCQTGHLIIDSECQTEFDNNNEIISLSLNYQDFSEESSGKIIKYFDDKEIQFDALCDSIP-KLHPIKSKKIQVGHTRLNDYREVECQSGC 3255

HSP 19 Score: 124.405 bits (311), Expect = 1.592e-33
Identity = 72/149 (48.32%), Postives = 96/149 (64.43%), Query Frame = 2 
Query:    5 EFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQS 451
            E MI     +N++  L I DLI+E  QY+++    +  + ++ KGKKLQVG P LD D EVSCQTG ++ +SECQT+L D  +T+  T H++DFSE+L + PMKE+ EKEIQ ET   S S       SK+VQVG  RL E  +V CQS
Sbjct: 5033 ESMIIVGTGRNVDQDLLIYDLIEETVQYNED----VNEAFKKLKGKKLQVGNPYLDSDSEVSCQTGHLLIESECQTELGDEFDTIIKTEHTQDFSEELMERPMKEFVEKEIQFETAKSSLSKYNTLENSKEVQVGQSRLVESREVGCQS 5177

HSP 20 Score: 108.612 bits (270), Expect = 6.226e-28
Identity = 69/150 (46.00%), Postives = 93/150 (62.00%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQS 451
            DE MI   +E + E    ++ +IDE TQY DE + V  + Q++ K KKLQVG  RLD D EVSCQTG  I DSECQT++ DS + + IT   ++F E+  K  ++E+ EKEIQ E      S   K + S+ +QVG  +LNE  +VECQS
Sbjct: 4875 DEIMIMTERENHFEERFVLDLMIDETTQYGDEII-VYCSLQKDLKNKKLQVGNSRLDIDSEVSCQTGNFIMDSECQTEVEDSTDMILITHQFQNFEEER-KPHVREFVEKEIQSEF----LSRYNKPSSSQVIQVGLSKLNEYKEVECQS 5018

HSP 21 Score: 106.686 bits (265), Expect = 3.135e-27
Identity = 67/153 (43.79%), Postives = 94/153 (61.44%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLN--DSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 454
            DEF+IEE + +N  A+LF+E++I+E+ QY  + +   I   +E + K  QVG PR++ D++ SCQ G ++++ E QTD    D  ET  ITM       +       E+ EKE+Q   PD  +    K+ KSKKVQVGHPRLN+ LDVECQSG
Sbjct: 1500 DEFVIEEIRNKNELAALFVEEMIEESVQYMADDI-FTIQYPKETQEKNFQVGNPRIEIDYDTSCQAGHLVRELEIQTDQEPFDGFET-QITMVKDQLESQNIVRTTTEFIEKEVQ---PDFVSE---KSRKSKKVQVGHPRLNDNLDVECQSG 1644

HSP 22 Score: 105.916 bits (263), Expect = 5.995e-27
Identity = 69/154 (44.81%), Postives = 98/154 (63.64%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTI--TMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGC 457
            DE + E   ++NLEA L  +++I+EATQ + + ++   + +    GKKLQVG  RL+ DF+ SCQTG I+KD   QT++ ++V ++ I   +   +F E   K+ +    E EIQ +     + + I  NKSKKVQVGHPRLNEILDVECQSGC
Sbjct: 1819 DELLHEVVIDRNLEAFLVNKEMINEATQCNFDLMQPH-DGKLMGIGKKLQVGTSRLEYDFDASCQTGHIVKDDCDQTEIEETVGSILIESKLALNEFQEMTSKDSIS-LREIEIQTDDFPGFSVNIINMNKSKKVQVGHPRLNEILDVECQSGC 1970

HSP 23 Score: 87.8113 bits (216), Expect = 1.039e-20
Identity = 60/155 (38.71%), Postives = 88/155 (56.77%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSV-----ETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQS 451
            DEF+IE+  + N   +   E++I+E  QY+ E ++ I   +   KGKKLQ G  RL+ DF+ SCQTG ++ +SE QT+ ND       + + +      F  +L     KEY ++E+Q +  DD T    +  K KKVQVG+PRLN  +  ECQS
Sbjct: 1657 DEFIIEQTTQINENLTQSSEEMIEELVQYESENLRPI-TLKTNTKGKKLQTGSSRLENDFDSSCQTGHLVTESEVQTE-NDEFGGKIDKEIRLANEQTQFQNELL---FKEYVDQEVQLDI-DDFTGPIPRNTKCKKVQVGYPRLNLQIHAECQS 1805

HSP 24 Score: 75.0998 bits (183), Expect = 2.855e-16
Identity = 54/135 (40.00%), Postives = 75/135 (55.56%), Query Frame = 2 
Query:   53 FIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEK--LFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQS 451
            F  +LIDE  QY    ++  + ++   +GK +Q G P+L  + + SCQ G +  ++ECQTD   S E L IT    +   +  L +   KEY EKEIQ        S     NK KK+QVGHP+LNE ++VECQS
Sbjct: 1358 FGLELIDEELQYYG-SLQDRLGNELFYEGKNVQSGSPKLFMELDESCQIGYVKHEAECQTDNEFSDEELVITEVQAELQSQNVLMESKKKEYTEKEIQPILTFYKAS-----NKGKKIQVGHPKLNEFVEVECQS 1486

HSP 25 Score: 64.6994 bits (156), Expect = 1.304e-12
Identity = 49/130 (37.69%), Postives = 69/130 (53.08%), Query Frame = 2 
Query:   62 DLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKAN-KSKKVQVGHPRLNEILDVECQ 448
            +L DE+TQ+   P+   I     +K KKLQ+G  RL+G+ E SCQTG ++ +   QT+                  E LF EPM     KEIQ +  + +   QI+   K KKVQVG+P+  + +DVECQ
Sbjct:  986 ELKDESTQWGIIPIDNEI-EHGMKKNKKLQIGNSRLEGNLESSCQTGHVVDEFHTQTE------------------ELLFMEPMG-LLSKEIQSDI-ESADKYQIRLEMKGKKVQVGYPQTYDRIDVECQ 1094

HSP 26 Score: 60.077 bits (144), Expect = 5.196e-11
Identity = 39/105 (37.14%), Postives = 54/105 (51.43%), Query Frame = 2 
Query:  134 KGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQ 448
            K KKLQ G  RL+ D+E SCQTG I+ + E QT+     E++                P KEY  KE Q E+       ++   K KK+QVG+P +N+I + E Q
Sbjct: 1250 KSKKLQAGSSRLNADYEASCQTGHIVNEIETQTEFFYKTESIL---------------P-KEYSNKETQPES-------EMAITKGKKIQVGYPHINKISEKEIQ 1331

HSP 27 Score: 59.6918 bits (143), Expect = 7.559e-11
Identity = 40/106 (37.74%), Postives = 50/106 (47.17%), Query Frame = 2 
Query:  137 GKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 454
             K+ Q+G  RLDGDFE SCQTG I+KD E QT+ ND                         Y E +IQ  T  ++        K KKVQVG+P+LN   +   Q G
Sbjct: 1128 NKETQIGCSRLDGDFETSCQTGHIVKDFESQTE-ND-------------------------YTEHQIQLVTMANNLQAVKSQMKGKKVQVGYPQLNMSFEFGSQFG 1207

HSP 28 Score: 58.151 bits (139), Expect = 2.217e-10
Identity = 41/135 (30.37%), Postives = 76/135 (56.30%), Query Frame = 2 
Query:   59 EDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSV--ETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKAN-KSKKVQVGHPRLNEILDVECQSG 454
            ++++   T++ ++ V+    S++ +K KK+QVG PRL+ + +V CQ+G ++ + E QT+L++ +  +T  I  +    SE++ +E ++  YE E       +     +K N K KK+Q G  RL    D  CQ+G
Sbjct: 1597 QNIVRTTTEFIEKEVQPDFVSEKSRKSKKVQVGHPRLNDNLDVECQSGTLLNEMEIQTELDEFIIEQTTQINENLTQSSEEMIEELVQ--YESE-------NLRPITLKTNTKGKKLQTGSSRLENDFDSSCQTG 1722

HSP 29 Score: 56.225 bits (134), Expect = 1.040e-9
Identity = 44/135 (32.59%), Postives = 71/135 (52.59%), Query Frame = 2 
Query:   53 FIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQI-KANKSKKVQVGHPRLNEILDVECQSG 454
            F+++  ++  QY D     +I+ +   K KK+QVG PRL+   +V CQTG ++ D E Q  L    E + +    ++    LF E +    E+  Q +  + + +  I K  K KK+QVG  RL+  L+V CQ+G
Sbjct: 4332 FLKEFAEKEVQYGDFDDYSLIHDEA-NKSKKVQVGHPRLNESLDVECQTGCLMDDIEVQCKLE---EFMIMENKVRNLEANLFIEDL---IEESTQYDEGNLNITTAIHKEVKGKKLQVGTSRLDSDLEVSCQTG 4459

HSP 30 Score: 55.4546 bits (132), Expect = 2.076e-9
Identity = 55/158 (34.81%), Postives = 79/158 (50.00%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDS--VETLTI-------TMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQ 448
            DE +I E  +QN+ + L  EDLI E  Q++    +  I  +++ KGKKLQVG  RL  D            D   QT+LNDS  +E L I         ++  FS+K   EP+   +++E++  T +          KS  VQVG+PRLN  +D E Q
Sbjct: 5498 DEALIIETTQQNVHSKLIFEDLIVEEIQFNCLLPRSSI--KKDSKGKKLQVGNSRLLDD-----------SDEFTQTELNDSRNIEDLCIQQVQSFQNTNTISFSDKEV-EPL---FDEEVEFPTTN--------VFKSHSVQVGYPRLNACVDFEGQ 5630

HSP 31 Score: 53.9138 bits (128), Expect = 6.076e-9
Identity = 40/112 (35.71%), Postives = 59/112 (52.68%), Query Frame = 2 
Query:  125 REQKGKKLQVGIPRLDGDFEVSCQTGRIIKD--SECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 454
            +  K KK+QVG PRL+ + EV CQ+G ++ D   +C+ D    +ET    + +  F E L  E   +Y +K +        T    K  K KK+QVG PRL+   +V CQ+G
Sbjct: 3877 KANKSKKVQVGHPRLNENLEVECQSGCLMDDIGVQCEVDEFMIMETKERNLEASLFVEDLIDEAT-QYDDKNLHF------TIAAHKDVKGKKLQVGTPRLDADPEVSCQTG 3981

HSP 32 Score: 53.5286 bits (127), Expect = 9.812e-9
Identity = 48/145 (33.10%), Postives = 77/145 (53.10%), Query Frame = 2 
Query:   53 FIEDLIDEATQYDDEPVKV----IINSQ--REQKGKKLQVGIPRLDGDFEVSCQTGRIIKD--SECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQI---KANKSKKVQVGHPRLNEILDVECQSG 454
            F E L  E   Y ++ ++     + +SQ  + +K KK+QVG PRL+   E  CQ+G I+ D   +C+TD     E +    + ++    L    + +  E+ IQ    D+S+S  +   K  K KK+QVG+PRL+  L+V CQ+G
Sbjct: 2874 FSEKLFKEPKNYLEKEIQFETAEVSDSQLVKTKKSKKIQVGHPRLNKSLEGECQSGCIMDDIGVQCETD-----EFMIEENNEQNLEASLL---IDDLIEENIQ--YSDESSSVTVLSQKEQKRKKLQVGNPRLDGDLEVSCQTG 3008

HSP 33 Score: 53.1434 bits (126), Expect = 1.144e-8
Identity = 37/112 (33.04%), Postives = 59/112 (52.68%), Query Frame = 2 
Query:  125 REQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSV--ETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 454
            +  K KK+QVG PRL+   +V CQ+G I+ D   Q ++++ +  E     + +  F E L  E  +  Y+ E     P     + ++  K KK+QVG PRL+   +V CQ+G
Sbjct: 2270 KANKSKKVQVGHPRLNEILDVECQSGYIMDDIGVQCEVDEFMIEENKEQNLEASLFIEDLIDEATQ--YDDE-----PVKGIINSLREQKGKKLQVGIPRLDGDFEVSCQTG 2374

HSP 34 Score: 53.1434 bits (126), Expect = 1.259e-8
Identity = 42/123 (34.15%), Postives = 63/123 (51.22%), Query Frame = 2 
Query:   98 PVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKE----YYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 454
            P   I    +  K KK+QVG PRL+   +V CQ+G I+ D   Q +++   E +      K+  E+LF E + E    Y E+ +       +   QI+  K KK+QVG PRL+   +V CQ+G
Sbjct: 2733 PNYSISEHNKANKSKKVQVGHPRLNEILDVECQSGCIMDDIGVQCEVD---EFMIEERKEKNLEERLFVEDLIEEATQYDEENLNV-----TIGAQIET-KGKKLQVGIPRLDVDFEVSCQTG 2846

HSP 35 Score: 52.7582 bits (125), Expect = 1.630e-8
Identity = 42/126 (33.33%), Postives = 64/126 (50.79%), Query Frame = 2 
Query:   83 QYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSV--ETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 454
            Q DD P    +N     K KK+QVG PRL+   +V CQ+G I+ D   Q ++++ +  E     + +  F E L  E  +  Y+ E     P     + ++  K KK+QVG PRL+   +V CQ+G
Sbjct: 1931 QTDDFP-GFSVNIINMNKSKKVQVGHPRLNEILDVECQSGCIMDDIGVQCEVDEFMIEENKEQNLEASLFIEDLIDEATQ--YDDE-----PVKGIINSLREQKGKKLQVGIPRLDGDFEVSCQTG 2048

HSP 36 Score: 50.0618 bits (118), Expect = 1.473e-7
Identity = 44/151 (29.14%), Postives = 75/151 (49.67%), Query Frame = 2 
Query:    2 DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 454
            +E +I E + +    ++ +E    E T+ + +P+     +    KGKK+QVG P+L+   EV CQ+   I++   QT+++   E +   + +K+    LF E M    E+ +Q    D  T    K  + K  QVG+PR+    D  CQ+G
Sbjct: 1423 EELVITEVQAELQSQNVLMESKKKEYTEKEIQPILTFYKASN--KGKKIQVGHPKLNEFVEVECQSEISIEEIAVQTEID---EFVIEEIRNKNELAALFVEEM---IEESVQYMADDIFTIQYPKETQEKNFQVGNPRIEIDYDTSCQAG 1565

HSP 37 Score: 49.6766 bits (117), Expect = 1.943e-7
Identity = 36/108 (33.33%), Postives = 59/108 (54.63%), Query Frame = 2 
Query:  134 KGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQI-KANKSKKVQVGHPRLNEILDVECQSG 454
            K KK+QVG  RL+   EV CQ+G I+ D E QT+++   E + +    ++  E LF     +  ++  Q +  + + +  + K  K KK+QVG  RL +  +V CQ+G
Sbjct: 3232 KSKKIQVGHTRLNDYREVECQSGCIMDDVEVQTEVD---EFMIMERKERNLEESLF---FDDLIDESTQFDGENLNVTVAVPKEIKGKKLQVGSARLYDDFEVSCQTG 3333

HSP 38 Score: 49.2914 bits (116), Expect = 2.286e-7
Identity = 38/115 (33.04%), Postives = 63/115 (54.78%), Query Frame = 2 
Query:  125 REQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFS---EKLFKEPMKEY--YEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 454
            +  K KK+QVG PRL+ + EV CQ+G ++ D   Q ++N+      + M +K+ +    +L  + ++E   Y+ E+        T+   K  K KK+QVG P+L   L+V CQ+G
Sbjct: 4041 KSSKSKKVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNE-----IMIMGNKEQNLEVSRLIDDLIEESTQYDGEVII-----VTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTG 4145

HSP 39 Score: 49.2914 bits (116), Expect = 2.638e-7
Identity = 37/112 (33.04%), Postives = 58/112 (51.79%), Query Frame = 2 
Query:  125 REQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSV--ETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 454
            +  K KK+QVG PRL+   +V CQ+G I+ D   Q ++++ +  E     + +  F E L  E  +  Y+ E     P     +  +  K KK+QVG PRL+   +V CQ+G
Sbjct: 2579 KANKSKKVQVGHPRLNEILDVECQSGCIMDDIGVQCEVDEFMIEENKEQNLEASLFIEDLIDEATQ--YDDE-----PVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTG 2683

HSP 40 Score: 48.9062 bits (115), Expect = 3.480e-7
Identity = 34/111 (30.63%), Postives = 59/111 (53.15%), Query Frame = 2 
Query:  125 REQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQI-KANKSKKVQVGHPRLNEILDVECQSG 454
            +  K KK+QVG PRL+ + EV CQ+G ++ D   Q ++    E + +    ++  E LF     +  ++  Q +  + + +  + K  K KK+QVG  RL +  +V CQ+G
Sbjct: 3553 KANKSKKVQVGHPRLNENLEVECQSGCLMDDIGVQCEVE---EFMIMERKERNLEESLF---FDDLIDESTQFDGENLNVTVAVPKEIKGKKLQVGSARLYDDFEVSCQTG 3657

HSP 41 Score: 48.1358 bits (113), Expect = 6.856e-7
Identity = 35/110 (31.82%), Postives = 52/110 (47.27%), Query Frame = 2 
Query:  128 EQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTD-LNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 454
            + KGKK+QVG P+L+  FE   Q G  + D+  QTD + + +  +T+T                   + EIQ    ++  S      KSKK+Q G  RLN   +  CQ+G
Sbjct: 1183 QMKGKKVQVGYPQLNMSFEFGSQFGDDLNDATIQTDSIEEYINPMTLT-------------------DGEIQWNDVEEG-SQSSSGLKSKKLQAGSSRLNADYEASCQTG 1272

HSP 42 Score: 47.7506 bits (112), Expect = 9.853e-7
Identity = 38/115 (33.04%), Postives = 62/115 (53.91%), Query Frame = 2 
Query:  125 REQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDF---SEKLFKEPMKEY--YEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 454
            +  K KK+QVG PRL+ + EV CQ+G ++ D   Q ++N+      + M SK+    + +L  + ++E   Y+ E+        T+   K  K KK+QVG  +L   L+V CQ+G
Sbjct: 3391 KASKSKKVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNE-----IMIMGSKEQNLEASRLIDDLIEESTQYDDEVII-----VTNAMQKEVKGKKLQVGTSKLEGDLEVSCQTG 3495

HSP 43 Score: 47.7506 bits (112), Expect = 9.853e-7
Identity = 38/115 (33.04%), Postives = 62/115 (53.91%), Query Frame = 2 
Query:  125 REQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDF---SEKLFKEPMKEY--YEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 454
            +  K KK+QVG PRL+ + EV CQ+G ++ D   Q ++N+      + M SK+    + +L  + ++E   Y+ E+        T+   K  K KK+QVG  +L   L+V CQ+G
Sbjct: 3715 KASKSKKVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNE-----IMIMGSKEQNLEASRLIDDLIEESTQYDDEVII-----VTNAMQKEVKGKKLQVGTSKLEGDLEVSCQTG 3819

HSP 44 Score: 47.3654 bits (111), Expect = 1.063e-6
Identity = 38/104 (36.54%), Postives = 56/104 (53.85%), Query Frame = 2 
Query:  143 KLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 454
            K+QVG PRLD + EV CQ+G I+ D E QT++ + +  L     S +F   LF + + E  +  +              A K KK+Q+G PRL+  L+V CQ+G
Sbjct: 4209 KVQVGFPRLDENREVECQSGSIMDDVEVQTEVVEVM-MLDNKERSLEFG-FLFDDLINESIQYNV--------------AVKGKKLQIGFPRLDGELEVSCQTG 4296

HSP 45 Score: 47.3654 bits (111), Expect = 1.350e-6
Identity = 43/129 (33.33%), Postives = 63/129 (48.84%), Query Frame = 2 
Query:   68 IDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 454
            ID   Q D E VK        QKGKK+Q+G  +L    E SCQ G ++ +SE QTD     + L   + + + S  +  E   E  +  I    P D+  +     K+KK+Q+G+ RL   L+  CQ+G
Sbjct:  920 IDVEIQSDLEIVK--------QKGKKVQIGSSKLLETCESSCQIGTVLHNSELQTD-----DKLKFVIQANEKSSPIGLELKDESTQWGI---IPIDNEIEH-GMKKNKKLQIGNSRLEGNLESSCQTG 1031

HSP 46 Score: 46.2098 bits (108), Expect = 2.894e-6
Identity = 39/144 (27.08%), Postives = 67/144 (46.53%), Query Frame = 2 
Query:   23 NKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 454
            N++   +  L  ++ +D+  Q D +     I   R  K KK+QVG PRL+      CQ+  ++++ + QT+ ++ +  + I  + + F   + KE + E  +       P D     I     KK+QVG  RL    D  CQ+G
Sbjct: 1749 NEQTQFQNELLFKEYVDQEVQLDIDDFTGPI--PRNTKCKKVQVGYPRLNLQIHAECQSEIVLQNEDIQTENDELLHEVVIDRNLEAF--LVNKEMINEATQCNFDLMQPHDGKLMGI----GKKLQVGTSRLEYDFDASCQTG 1884

HSP 47 Score: 45.8246 bits (107), Expect = 4.318e-6
Identity = 45/153 (29.41%), Postives = 74/153 (48.37%), Query Frame = 2 
Query:   23 NKEQNLEASLFIEDLIDEATQYDDEPVKVII---------NSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 454
            NK+ +L+     ED +DE       P K +I         +S  ++K K++Q G  +L G+ E S QT   + ++  QT+ N+  +  T++ +     E L +EP K+  + EIQ +        +I   K KKVQ+G  +L E  +  CQ G
Sbjct:  813 NKDADLQTDPIGEDFLDEVDLLRSGPRKTVIYIRDNSTDMDSIGQRKNKRIQFGKSKLFGEGEFSSQTNYHLIEANVQTN-NEVEDYATLSENGMLEYEILTQEP-KQTIDVEIQSDL-------EIVKQKGKKVQIGSSKLLETCESSCQIG 956

HSP 48 Score: 45.8246 bits (107), Expect = 4.528e-6
Identity = 32/106 (30.19%), Postives = 57/106 (53.77%), Query Frame = 2 
Query:  143 KLQVGIPRLDGDFEVSCQTGRIIKDSECQTD---LNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQS 451
            KLQ G  RL G+ ++ CQTG ++ D+ECQT+    ND +E     +++ +        P K    +E Q     D   + + + +++ +QVG P+L++  ++ CQS
Sbjct: 5236 KLQAGSSRLLGEMDLLCQTGHLMLDNECQTNKMTTNDIMENFQNELNADE------SFPTKSLSNQESQY----DLCEETVLSCQNQALQVGFPKLHQSQEISCQS 5331

HSP 49 Score: 45.0542 bits (105), Expect = 7.084e-6
Identity = 35/113 (30.97%), Postives = 59/113 (52.21%), Query Frame = 2 
Query:  119 SQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLFKEPMKEYYEKEIQCETPD-DSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 454
            S +  K KK+QVG  +L    EV CQ+G ++ +   Q +L+   E + +    ++    L  E +    E+  Q +  + + T   +K  K KK+QVG  +L+E L+V CQ+G
Sbjct: 4516 SDKSNKSKKVQVGHAKLHEGVEVECQSGYMMDEIGVQCELD---EFMIMENKERNLEASLLIEDL---IEESTQYDDENLNVTISVLKETKGKKLQVGCSKLDEDLEVSCQTG 4622
The following BLAST results are available for this feature:
BLAST of SMED30012409 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012409 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012409 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012409 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012409 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012409 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012409 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012409 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012409 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012409 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012409 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012409 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012409 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012409 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000023005.12.026e-9698.03SMESG000023005.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012409 ID=SMED30012409|Name=SMED30012409|organism=Schmidtea mediterranea sexual|type=transcript|length=458bp
AGATGAATTCATGATTGAGGAAAATAAAGAACAAAATCTTGAAGCAAGTC
TGTTTATTGAAGATCTCATTGATGAAGCAACTCAGTATGATGATGAACCA
GTAAAAGTGATAATTAATTCACAGAGAGAACAGAAAGGAAAGAAATTGCA
AGTAGGAATTCCAAGATTGGACGGCGATTTTGAAGTGTCATGTCAAACCG
GGCGAATAATAAAAGATTCTGAGTGCCAGACCGACCTGAATGATTCTGTT
GAGACATTGACGATAACGATGCATTCTAAAGATTTCAGTGAAAAATTATT
CAAAGAACCGATGAAAGAATATTATGAAAAAGAAATCCAATGTGAAACAC
CGGATGATTCCACATCAGATCAAATAAAAGCCAATAAATCAAAGAAAGTT
CAAGTGGGTCATCCAAGGTTAAATGAAATTCTAGATGTCGAATGTCAGTC
AGGATGCA
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protein sequence of SMED30012409-orf-1

>SMED30012409-orf-1 ID=SMED30012409-orf-1|Name=SMED30012409-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=152bp
DEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQ
VGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFSEKLF
KEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQS
GC
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte