SMED30012398
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30012398 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 28Note: Hover over icons to view figure legend
Alignments
SMED30012398 aligns in the following genomic locations:
Homology
BLAST of SMED30012398 vs. Planmine SMEST
Match: SMESG000042071.1 (SMESG000042071.1) HSP 1 Score: 238.424 bits (607), Expect = 9.156e-81 Identity = 117/118 (99.15%), Postives = 117/118 (99.15%), Query Frame = 2 Query: 161 MLLKAFLFIAITVVNIQLRTVESISCKICSPCWNSTSDDQYDDGFLNGNMRITGCKSCRTNIKNNIIMKSCSEETCDNKEAGEECCTEELCNAAKNSITAKSNAPRKYKRKKCYFNFA 514 M LKAFLFIAITVVNIQLRTVESISCKICSPCWNSTSDDQYDDGFLNGNMRITGCKSCRTNIKNNIIMKSCSEETCDNKEAGEECCTEELCNAAKNSITAKSNAPRKYKRKKCYFNFA Sbjct: 1 MQLKAFLFIAITVVNIQLRTVESISCKICSPCWNSTSDDQYDDGFLNGNMRITGCKSCRTNIKNNIIMKSCSEETCDNKEAGEECCTEELCNAAKNSITAKSNAPRKYKRKKCYFNFA 118
BLAST of SMED30012398 vs. Planmine SMEST
Match: SMESG000042755.1 (SMESG000042755.1) HSP 1 Score: 231.491 bits (589), Expect = 4.006e-78 Identity = 113/118 (95.76%), Postives = 116/118 (98.31%), Query Frame = 2 Query: 161 MLLKAFLFIAITVVNIQLRTVESISCKICSPCWNSTSDDQYDDGFLNGNMRITGCKSCRTNIKNNIIMKSCSEETCDNKEAGEECCTEELCNAAKNSITAKSNAPRKYKRKKCYFNFA 514 M LKAFLFIAIT+VNIQLRTVESISCKICSPCWNSTSDDQYDDGFLNGNMR++GCKSCRTNIKNNIIMKSCSEETCDNKEAGEECCTEELCNAAKNSITAKSNAPRKYKRKK YFNFA Sbjct: 1 MQLKAFLFIAITLVNIQLRTVESISCKICSPCWNSTSDDQYDDGFLNGNMRLSGCKSCRTNIKNNIIMKSCSEETCDNKEAGEECCTEELCNAAKNSITAKSNAPRKYKRKKFYFNFA 118
BLAST of SMED30012398 vs. Planmine SMEST
Match: SMESG000042052.1 (SMESG000042052.1) HSP 1 Score: 200.675 bits (509), Expect = 2.498e-76 Identity = 98/110 (89.09%), Postives = 102/110 (92.73%), Query Frame = 2 Query: 161 MLLKAFLFIAITVVNIQLRTVESISCKICSPCWNSTSDDQYDDGFLNGNMRITGCKSCRTNIKNNIIMKSCSEETCDNKEAGEECCTEELCNAAKNSITAKSNAPRKYKR 490 M LKAFLFIAITVVNIQLRTVESISCKIC+PCWNSTSDDQY DGF NGNMR++GCKSCRTNIKNNIIMKSCSEETCD KEAGEECCTEELCN+AKN TAKSNA KYKR Sbjct: 1 MQLKAFLFIAITVVNIQLRTVESISCKICNPCWNSTSDDQYSDGFSNGNMRLSGCKSCRTNIKNNIIMKSCSEETCDKKEAGEECCTEELCNSAKNRKTAKSNARSKYKR 110 HSP 2 Score: 92.8189 bits (229), Expect = 2.498e-76 Identity = 44/47 (93.62%), Postives = 44/47 (93.62%), Query Frame = 1 Query: 592 SANPFIFEQFISSFDTIQQHRVVLFNEIIHYSLFIYLYQEMKFFTNK 732 ANPFI EQFISSFDTIQQHRVVLFNEIIHY LFIYLYQEMKFFTNK Sbjct: 128 CANPFISEQFISSFDTIQQHRVVLFNEIIHYILFIYLYQEMKFFTNK 174 HSP 3 Score: 32.3426 bits (72), Expect = 2.498e-76 Identity = 16/19 (84.21%), Postives = 17/19 (89.47%), Query Frame = 3 Query: 489 ERNVISILPEFNRIICSAV 545 +RNVISIL EFN IICSAV Sbjct: 109 KRNVISILLEFNGIICSAV 127
BLAST of SMED30012398 vs. Planmine SMEST
Match: SMESG000042059.1 (SMESG000042059.1) HSP 1 Score: 195.667 bits (496), Expect = 1.607e-73 Identity = 97/110 (88.18%), Postives = 100/110 (90.91%), Query Frame = 2 Query: 161 MLLKAFLFIAITVVNIQLRTVESISCKICSPCWNSTSDDQYDDGFLNGNMRITGCKSCRTNIKNNIIMKSCSEETCDNKEAGEECCTEELCNAAKNSITAKSNAPRKYKR 490 M LKAFLFIAITVVNIQLRTVESISC IC+PCW+STSDDQY DGF N NMRITGCKSCRTNIKNNIIMKSCSEETCD KEAGEECCTEELCN+AKN TAKSNA KYKR Sbjct: 1 MQLKAFLFIAITVVNIQLRTVESISCNICNPCWSSTSDDQYSDGFSNENMRITGCKSCRTNIKNNIIMKSCSEETCDKKEAGEECCTEELCNSAKNRKTAKSNARSKYKR 110 HSP 2 Score: 88.5817 bits (218), Expect = 1.607e-73 Identity = 42/47 (89.36%), Postives = 43/47 (91.49%), Query Frame = 1 Query: 592 SANPFIFEQFISSFDTIQQHRVVLFNEIIHYSLFIYLYQEMKFFTNK 732 ANPFI EQFISSFDTI+QHRVVLFNEIIHY LFIY YQEMKFFTNK Sbjct: 128 CANPFISEQFISSFDTIRQHRVVLFNEIIHYILFIYFYQEMKFFTNK 174 HSP 3 Score: 31.9574 bits (71), Expect = 1.607e-73 Identity = 16/19 (84.21%), Postives = 17/19 (89.47%), Query Frame = 3 Query: 489 ERNVISILPEFNRIICSAV 545 +RNVISIL EFN IICSAV Sbjct: 109 KRNVISILLEFNGIICSAV 127
BLAST of SMED30012398 vs. Planmine SMEST
Match: SMESG000057926.1 (SMESG000057926.1) HSP 1 Score: 103.219 bits (256), Expect = 6.432e-31 Identity = 57/100 (57.00%), Postives = 63/100 (63.00%), Query Frame = 2 Query: 191 ITVVNIQLRTVESISCKICSPCWNSTSDDQYDDGFLNGNMRITGCKSCRTNIKNNIIMKSCSEETCDNKEAGEECCTEELCNA-----AKNSITAKSNAP 475 IT N QL TV SISC IC+PCWNSTSD QY DGFLN N+ I+G C+ TC+NKEAGEECCTEE CN+ A N ITAK NAP Sbjct: 110 ITAKNTQLGTVHSISCHICNPCWNSTSDYQYTDGFLNQNLLISG----------------CNNRTCENKEAGEECCTEEQCNSAMKSNAPNGITAKGNAP 193 HSP 2 Score: 49.2914 bits (116), Expect = 6.432e-31 Identity = 25/31 (80.65%), Postives = 27/31 (87.10%), Query Frame = 3 Query: 480 NIKERNVISILPEFNRIICSAVWLINSNILF 572 N ERN+ISIL EFNRIICS VWLI+SNILF Sbjct: 224 NKYERNIISILLEFNRIICSVVWLIHSNILF 254 HSP 3 Score: 108.612 bits (270), Expect = 2.531e-28 Identity = 60/93 (64.52%), Postives = 63/93 (67.74%), Query Frame = 2 Query: 161 MLLKAFLFIAITVVNIQLRTVESISCKICSPCWNSTSDDQYDDGFLNGNMRITGCKSCRTNIKNNIIMKSCSEETCDNKEAGEECCTEELCNA 439 M LKAFLFIAI V NIQL TV SISC C CWNS DD ++ R CKSCR IKNNI+ KSCSE TCDNK A EECCTEE CNA Sbjct: 1 MRLKAFLFIAIAVFNIQLGTVHSISCIKCGLCWNSDRDD------MDQTSRQHNCKSCRKIIKNNILSKSCSENTCDNKGADEECCTEEFCNA 87 The following BLAST results are available for this feature:
BLAST of SMED30012398 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30012398 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30012398 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30012398 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30012398 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30012398 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30012398 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30012398 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30012398 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30012398 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30012398 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30012398 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30012398 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30012398 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30012398 ID=SMED30012398|Name=SMED30012398|organism=Schmidtea mediterranea sexual|type=transcript|length=748bpback to top protein sequence of SMED30012398-orf-1 >SMED30012398-orf-1 ID=SMED30012398-orf-1|Name=SMED30012398-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=119bp MLLKAFLFIAITVVNIQLRTVESISCKICSPCWNSTSDDQYDDGFLNGNMback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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