SMED30012261

Overview
NameSMED30012261
Smed IDSMED30012261
Length (bp)1799
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30012261 (SMED30012261) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30012261 (SMED30012261) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30012261

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
testis primordiumSMED30012261SMESG000071248.1 Contig4780_SE3GPL10652PMID:20844018
Wang et al., 2010
whole organism juvenile cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Alignments
SMED30012261 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000586:49963..51769 +8756
Homology
BLAST of SMED30012261 vs. Planmine SMEST
Match: SMESG000071248.1 (SMESG000071248.1)

HSP 1 Score: 765.377 bits (1975), Expect = 0.000e+0
Identity = 463/466 (99.36%), Postives = 465/466 (99.79%), Query Frame = 1
Query:   76 MFDNTKESDNVGQVTEVPVRDLAREEFFDVTDRKLFDTKVRREYLGYKLLLDIXXXXXXXXXXXXXXXXXXXXXXXXXSKIETLETRIKNLKEQAVRIRKQTELERQLMADSKRTQAMRLNDDQTRYKQRLQHAKAIYEDWDKLKEIENRIKREENCEEKFWLEKNSERIEDLIRXXXXXXXXXXXXXXDFKQQIXXXXXXXXXXXXXXXXNQAFLNEMSKEVDKQIAEEDALVKKKKAMKQSDYHKLLDNQIRIKRAINDRNKSTEEALELILKDENQQKIKSNQAKNQQAIRNNFKSFTNFMKYQDCLKKTREEEEKEINDNVMKTQIAIHRKNYEIEKEKVKKFAKLISEIKIAQKDQLVEASKLKNLIVEEKVKDRELVNKIYEEGLIQDKIKLEKERKKHESHFQLLKQQLVFNEEMRNFCKAEKDRERKSMNEAEKNYVEKIEEIRSKEQLQFIHPWRAH 1473
            MFDNTKESDNVGQVTEVPVRDLAREEFFDVTDRKLFDTKVRREYLGYKLLLDINLEERRGKLRRLLEENENNLTEEHLSKIETLETRIKNLKEQAVRIRKQTELERQLMADSKRTQAMRLNDDQTRYKQRLQHAKAIYEDWDKLKEIENRIKREENCEEKFWLEKNSERIEDLIRKENIVKNKNNAIKNDFKQQIEMREKQRQIEKEEKLENQAFLNEMSKEVDKQIAEEDALVKKKKAMKQSDYHKLLDNQIRIK+AINDRNKSTEE+LELILKDENQQKIKSNQAKNQQAIRNNFKSFTNFMKYQDCLKKTREEEEKEINDNVMKTQIAIHRKNYEIEKEKVKKFAKLISEIKIAQKDQLVEASKLKNLIVEEKVKDRELVNKIYEEGLIQDKIKLEKERKKHESHFQLLKQQLVFNEEMRNFCKAEKDRERKSMNEAEKNYV KIEEIRSKEQLQFIHPWRAH
Sbjct:    1 MFDNTKESDNVGQVTEVPVRDLAREEFFDVTDRKLFDTKVRREYLGYKLLLDINLEERRGKLRRLLEENENNLTEEHLSKIETLETRIKNLKEQAVRIRKQTELERQLMADSKRTQAMRLNDDQTRYKQRLQHAKAIYEDWDKLKEIENRIKREENCEEKFWLEKNSERIEDLIRKENIVKNKNNAIKNDFKQQIEMREKQRQIEKEEKLENQAFLNEMSKEVDKQIAEEDALVKKKKAMKQSDYHKLLDNQIRIKKAINDRNKSTEESLELILKDENQQKIKSNQAKNQQAIRNNFKSFTNFMKYQDCLKKTREEEEKEINDNVMKTQIAIHRKNYEIEKEKVKKFAKLISEIKIAQKDQLVEASKLKNLIVEEKVKDRELVNKIYEEGLIQDKIKLEKERKKHESHFQLLKQQLVFNEEMRNFCKAEKDRERKSMNEAEKNYVVKIEEIRSKEQLQFIHPWRAH 466          
BLAST of SMED30012261 vs. Planmine SMEST
Match: SMESG000072874.1 (SMESG000072874.1)

HSP 1 Score: 144.05 bits (362), Expect = 5.864e-37
Identity = 157/441 (35.60%), Postives = 256/441 (58.05%), Query Frame = 1
Query:  148 EEFFDVTDRKLFDTKVRREYLGYKLLLDIXXXXXXXXXXXXXXXXXXXXXXXXXSKIETLETRIKNLKEQAVRIRKQTELERQLMADSKRTQAMRLNDDQTRYKQRLQHAKAIYEDWDKLKEIENRIKREENCEEKFWLEKNSERIEDLIRXXXXXXXXXXXXXXDFKQQIXXXXXXXXXXXXXXXXNQAFLNEMSKEVDKQIAEEDALVKKKKAMKQSDYHKLLDNQIRIKRAINDRNKSTEEALELILKDENQQKIKSNQAKNQQAIRNNFKSFTNFMKYQDCLKKTREEEEKEINDNVMKTQIAIHRKNYEIEKEKVKKFAKLISEIKIAQKDQLVEASKLKNLIVEEKVKDRELVNKIYEEGLIQDKIKLEKERKKHESHFQLLKQQLVFNEEMRNFCKAEKDRERKSMNEAEKNYVEKIEEIRSKEQLQFIHPWRA 1470
            E+F + ++ K F  K+ RE     +LL INLEERR +LR LL+E +  + EEH ++IET++ R   LKE A+RIR Q E+ER+  A+ K  Q  RLND++ R  QR  H   I +DW+KLK+I+ RI++EE CE  FW EK  E    ++ +ENI   K     ++   QI+ RE QR +++E++LE   F  + +KE+  ++ +E    K  +   + ++ K LD  I++K    D +K  E A + I+ DEN  K+K +     +  R   K   N++  Q+C+K+ +E + K I+  V K  I  ++K  E++  + KK+A LI  +  +++D L +  + K   V E  K+++ +N+  +  + +++ K EKE++K   +   L  Q+ FN  +    K EK RE  SM EAE+ Y++ ++EI+ KE+L F+HPWR+
Sbjct:   26 EDFKNRSEMKNFSNKIIREIRSTNILLQINLEERRNRLRNLLDEEDRAIAEEHTTRIETIDRRRDRLKEYAMRIRYQKEIERKAEANEKYFQRDRLNDNEIRTAQRDIHFLEIIKDWEKLKDIKERIRKEERCESDFWAEKMKEERNKMLERENIEAAKLKIRSDEIMNQIKEREHQRALDREKQLEQIQFNFKNAKELQSKLEQEKIDRKANEKATKQNFRKSLDRHIQMKNRRLDEDKKRENAFDTIMMDENSLKMKESSYDAYKKKRETLKHLKNYIDRQNCIKEEKEADNKLIDQMVKKADIENNKKFRELQYLQRKKYADLIKSMNQSREDHLKKIEEKKVEKVIEIQKEKDAMNEFIKIEMEKERKKKEKEKEKINVYIDALSNQIKFNSHVAGMKKEEKIRELNSMKEAEQIYLKTLQEIKDKEELLFVHPWRS 466          
The following BLAST results are available for this feature:
BLAST of SMED30012261 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012261 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012261 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012261 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012261 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012261 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012261 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012261 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012261 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012261 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012261 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012261 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012261 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012261 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000071248.10.000e+099.36SMESG000071248.1[more]
SMESG000072874.15.864e-3735.60SMESG000072874.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012261 ID=SMED30012261|Name=SMED30012261|organism=Schmidtea mediterranea sexual|type=transcript|length=1799bp
ATGTTTGATAACACAAAGGAGTCAAACATTGTCAGACTCCTTTGTGTTAT
CAAACAAAAATCGATGAAAAAATTAATGTTTGATAACACAAAGGAGTCTG
ACAATGTTGGTCAAGTTACTGAAGTTCCAGTACGCGACTTAGCCAGAGAG
GAATTTTTCGATGTAACTGATCGGAAATTGTTTGACACAAAAGTTCGACG
AGAATACTTGGGATATAAATTACTCCTGGATATAAATTTAGAAGAGAGAC
GTGGAAAACTACGAAGACTATTGGAGGAAAATGAAAATAATTTAACAGAA
GAACATCTTTCTAAAATTGAAACATTGGAAACTAGAATTAAAAATCTAAA
AGAACAAGCTGTTAGAATTCGAAAACAGACAGAATTAGAACGGCAATTGA
TGGCTGATTCAAAAAGAACACAAGCAATGCGTTTAAACGACGATCAAACA
CGTTATAAACAACGTTTGCAACATGCTAAAGCTATTTATGAAGATTGGGA
TAAATTAAAAGAAATTGAAAATCGTATCAAACGCGAAGAAAACTGTGAGG
AAAAGTTCTGGTTAGAAAAAAATTCCGAAAGAATTGAGGATCTTATTCGA
AAAGAGAATATTGTCAAAAATAAAAACAATGCTATTAAAAACGATTTTAA
ACAGCAAATAGAAATGAGAGAAAAACAAAGGCAAATTGAAAAGGAAGAAA
AATTAGAGAACCAAGCGTTTTTAAATGAAATGTCAAAAGAAGTTGATAAG
CAAATTGCTGAAGAAGATGCATTAGTGAAAAAGAAAAAGGCCATGAAGCA
GTCCGATTATCATAAATTGTTGGACAACCAAATAAGAATTAAAAGGGCCA
TAAATGACAGAAATAAGTCAACTGAAGAAGCGCTTGAATTAATATTGAAA
GATGAAAATCAGCAAAAAATTAAAAGTAACCAGGCTAAAAACCAACAAGC
TATCCGAAACAATTTCAAGTCGTTTACTAATTTTATGAAATATCAAGACT
GTTTGAAAAAGACTAGGGAAGAAGAAGAGAAAGAAATCAACGACAATGTA
ATGAAAACTCAAATAGCGATTCATCGAAAAAATTATGAAATCGAAAAAGA
AAAGGTTAAAAAATTTGCTAAATTGATTTCAGAAATAAAAATTGCTCAAA
AAGATCAGTTAGTCGAGGCGTCAAAGTTGAAGAACTTAATTGTTGAAGAA
AAAGTTAAAGACCGCGAATTGGTAAATAAAATTTACGAAGAAGGTTTAAT
ACAAGATAAAATAAAGTTAGAAAAAGAAAGGAAAAAACATGAAAGTCACT
TCCAATTATTAAAACAACAATTAGTTTTTAATGAAGAAATGAGGAATTTT
TGCAAAGCGGAGAAAGACAGGGAAAGAAAATCAATGAATGAAGCTGAAAA
GAATTATGTAGAGAAAATAGAAGAAATTCGTTCAAAAGAACAACTTCAGT
TTATACATCCATGGCGAGCTCATTGATGAGCTTAGTACATAGGCATAAAG
TACCTTTTGGATGAAAGGCATATTTATTGTAAGATTTGTAAGTATATAAA
GCTTATGTAGAATATAGGAATTTATGAGATTTGACTTCAATATCAAGAAT
TTGGCTAATTCTGGAGAAATTCTTTTAATATTATTAACGTCAAAATTTTA
TTAACAAGGGATTCCCAGGGTGATAATCTCAGATCTAAACCAAATTTTGT
GTAAAAATGCTGGTTTTGTAATAAAGTTAGCTAAAGATGAGCTTAATAAT
CATCTCTAAATATTAAATTTAGTGGTAATCTAAGATTATCCCTTTACTG
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protein sequence of SMED30012261-orf-1

>SMED30012261-orf-1 ID=SMED30012261-orf-1|Name=SMED30012261-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=492bp
MFDNTKESNIVRLLCVIKQKSMKKLMFDNTKESDNVGQVTEVPVRDLARE
EFFDVTDRKLFDTKVRREYLGYKLLLDINLEERRGKLRRLLEENENNLTE
EHLSKIETLETRIKNLKEQAVRIRKQTELERQLMADSKRTQAMRLNDDQT
RYKQRLQHAKAIYEDWDKLKEIENRIKREENCEEKFWLEKNSERIEDLIR
KENIVKNKNNAIKNDFKQQIEMREKQRQIEKEEKLENQAFLNEMSKEVDK
QIAEEDALVKKKKAMKQSDYHKLLDNQIRIKRAINDRNKSTEEALELILK
DENQQKIKSNQAKNQQAIRNNFKSFTNFMKYQDCLKKTREEEEKEINDNV
MKTQIAIHRKNYEIEKEKVKKFAKLISEIKIAQKDQLVEASKLKNLIVEE
KVKDRELVNKIYEEGLIQDKIKLEKERKKHESHFQLLKQQLVFNEEMRNF
CKAEKDRERKSMNEAEKNYVEKIEEIRSKEQLQFIHPWRAH*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003304testis primordium
Vocabulary: INTERPRO
TermDefinition
IPR033365CFAP53
Vocabulary: biological process
TermDefinition
GO:0060271cilium morphogenesis
GO:0003341cilium movement
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 403..423
NoneNo IPR availableCOILSCoilCoilcoord: 431..465
NoneNo IPR availableCOILSCoilCoilcoord: 196..216
NoneNo IPR availableCOILSCoilCoilcoord: 82..109
NoneNo IPR availablePANTHERPTHR31183FAMILY NOT NAMEDcoord: 32..473
IPR033365Cilia- and flagella-associated protein 53PANTHERPTHR31183:SF1CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 53coord: 32..473