Peptidase M60 domain-containing protein

Overview


Name	Peptidase M60 domain-containing protein
Smed ID	SMED30012259
Length (bp)	569

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Peptidase M60 domain-containing protein (SMED30012259) t-SNE clustered cells

Violin plots show distribution of expression levels for Peptidase M60 domain-containing protein (SMED30012259) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Peptidase M60 domain-containing protein (SMED30012259) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Peptidase M60 domain-containing protein (SMED30012259) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30012259

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 9

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
parapharyngeal region	SMED30012259	h1SMcG0015035	SmedASXL_018243	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
parapharyngeal region	SMED30012259	h1SMcG0015035	SmedASXL_018244	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
nervous system	SMED30012259	h1SMcG0015035	dd_Smed_v4_13298_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30012259	h1SMcG0015035	dd_Smed_v4_13298_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parapharyngeal region	SMED30012259	h1SMcG0015035	dd_Smed_v4_13298_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
non-ciliated neuron	SMED30012259	h1SMcG0015035	dd_Smed_v4_13298_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30012259	h1SMcG0015035	Contig24476	newmark_ests	PMID:29674431 Fincher et al., 2018
parapharyngeal region	SMED30012259	h1SMcG0015035	dd_Smed_v6_13298_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
head region	SMED30012259	h1SMcG0015035	OX_Smed_1.0.10045	ox_Smed_v2	PMID:24238224 Kao et al., 2013

Note: Hover over icons to view figure legend

Homology

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Human
Match: TCAF2C (TRPM8 channel associated factor 2C [Source:HGNC Symbol;Acc:HGNC:53641])

HSP 1 Score: 71.2478 bits (173), Expect = 3.883e-14
Identity = 42/144 (29.17%), Postives = 75/144 (52.08%), Query Frame = 1

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENA---KFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFP 429
            W F   T E T NL+ ++ +  +      + +E++      + +++K +L + A    +++WT     AL  Y Q+   FG+  F ++FA Y   +  PK ++ +MN W+   S  V +NL+P F  WG+PI +E+ + L S P
Sbjct:  561 WEFPPHTTEATCNLWSVYVHETVLGIPRAQAHEAL--SPPERERRIKAHLGKGAPLCDWNVWT-----ALETYLQLQQAFGWEPFTQLFAEYQTLSHLPKDNTGRMNLWVKKFSEKVKKNLVPFFEAWGWPIQKEVADSLASLP 697

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Human
Match: TCAF2 (TRPM8 channel associated factor 2 [Source:HGNC Symbol;Acc:HGNC:26878])

HSP 1 Score: 71.2478 bits (173), Expect = 4.341e-14
Identity = 42/144 (29.17%), Postives = 75/144 (52.08%), Query Frame = 1

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENA---KFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFP 429
            W F   T E T NL+ ++ +  +      + +E++      + +++K +L + A    +++WT     AL  Y Q+   FG+  F ++FA Y   +  PK ++ +MN W+   S  V +NL+P F  WG+PI +E+ + L S P
Sbjct:  664 WEFPPHTTEATCNLWSVYVHETVLGIPRAQAHEAL--SPPERERRIKAHLGKGAPLCDWNVWT-----ALETYLQLQEAFGWEPFTQLFAEYQTLSHLPKDNTGRMNLWVKKFSEKVKKNLVPFFEAWGWPIQKEVADSLASLP 800

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Query:   10 WTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENA---KFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFP 429
            W F   T E T NL+ ++ +  +      + +E++      + +++K +L + A    +++WT     AL  Y Q+   FG+  F ++FA Y   +  PK ++ +MN W+   S  V +NL+P F  WG+PI +E+ + L S P
Sbjct:  664 WEFPPHTTEATCNLWSVYVHETVLGIPRAQAHEAL--SPPERERRIKAHLGKGAPLCDWNVWT-----ALETYLQLQEAFGWEPFTQLFAEYQTLSHLPKDNTGRMNLWVKKFSEKVKKNLVPFFEAWGWPIQKEVADSLASLP 800

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Human
Match: TCAF2 (TRPM8 channel associated factor 2 [Source:HGNC Symbol;Acc:HGNC:26878])

HSP 1 Score: 71.2478 bits (173), Expect = 4.426e-14
Identity = 42/144 (29.17%), Postives = 75/144 (52.08%), Query Frame = 1

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENA---KFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFP 429
            W F   T E T NL+ ++ +  +      + +E++      + +++K +L + A    +++WT     AL  Y Q+   FG+  F ++FA Y   +  PK ++ +MN W+   S  V +NL+P F  WG+PI +E+ + L S P
Sbjct:  768 WEFPPHTTEATCNLWSVYVHETVLGIPRAQAHEAL--SPPERERRIKAHLGKGAPLCDWNVWT-----ALETYLQLQEAFGWEPFTQLFAEYQTLSHLPKDNTGRMNLWVKKFSEKVKKNLVPFFEAWGWPIQKEVADSLASLP 904

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Human
Match: TCAF1 (TRPM8 channel associated factor 1 [Source:HGNC Symbol;Acc:HGNC:22201])

HSP 1 Score: 66.6254 bits (161), Expect = 1.601e-12
Identity = 41/141 (29.08%), Postives = 66/141 (46.81%), Query Frame = 1

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFP 429
            W F   T E T NL+C++ +  +     +  N +++     + K+V+ YL +      W +  + AL  Y Q+   FG+  F  +F  Y      P  + DKMN W+ + S  V +NL P F  W +PI +E+   L   P
Sbjct:  768 WEFPPHTTEATCNLWCVYVHETVLGIPRSRANIALWPPV--REKRVRIYLSKGPNVKNWNA--WTALETYLQLQEAFGWEPFIRLFTEYRNQTNLPTENVDKMNLWVKMFSHQVQKNLAPFFEAWAWPIQKEVATSLAYLP 904

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Zebrafish
Match: si:ch211-210b2.3 (si:ch211-210b2.3 [Source:ZFIN;Acc:ZDB-GENE-131122-13])

HSP 1 Score: 66.6254 bits (161), Expect = 9.428e-13
Identity = 40/143 (27.97%), Postives = 68/143 (47.55%), Query Frame = 1

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCF 435
            W F   T E T NL+ ++ + K+      + +  +  Q+  + +++K+Y      F+ W +  + AL  Y Q+   FG+  FK+VF+ Y      P  ++ KMN +    S++V  NL P F  WG+PI       L   P +
Sbjct:  765 WEFPPHTTECTCNLWSVYVHEKVLGVNKAKAHPCMTLQS--RQERIKEYFSGGKDFENWNT--WTALETYMQLQEKFGWDAFKKVFSLYHDMKDVPNDNAGKMNLYAKTFSKVVNHNLSPFFKVWGWPIQPNTEQELSHLPAW 903

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Zebrafish
Match: si:ch211-210b2.2 (si:ch211-210b2.2 [Source:ZFIN;Acc:ZDB-GENE-131118-33])

HSP 1 Score: 62.3882 bits (150), Expect = 2.639e-11
Identity = 40/143 (27.97%), Postives = 62/143 (43.36%), Query Frame = 1

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCF 435
            W F   T E T NL+ ++ + K+      + +  +  ++      +KKY         WT     AL  Y Q+   FG+  FK+VF+ Y      P  ++ KMN +    S++V  NL P F  WG+PI       L   P +
Sbjct:  761 WEFPPHTTECTCNLWSVYVHEKVLGVNKAKAHPCMTLESRQAC--IKKYFSGGKDLKNWTKAT--ALETYMQLQEKFGWDAFKKVFSLYHDMKDVPNDNAGKMNLYAKTFSKVVNHNLSPFFKVWGWPIQPNTEQELSHLPAW 899

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Zebrafish
Match: si:ch211-210b2.4 (si:ch211-210b2.4 [Source:ZFIN;Acc:ZDB-GENE-131122-12])

HSP 1 Score: 62.003 bits (149), Expect = 4.050e-11
Identity = 40/143 (27.97%), Postives = 62/143 (43.36%), Query Frame = 1

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCF 435
            W F   T E T NL+ ++ + K+      + +  +  ++      +KKY         WT     AL  Y Q+   FG+  FK+VF+ Y      P  ++ KMN +    S++V  NL P F  WG+PI       L   P +
Sbjct:  765 WEFPPHTTECTCNLWSVYVHEKVLGVNRAKAHPCMTLESRQAC--IKKYFSGGKDLKNWTKAT--ALETYMQLQEKFGWDAFKKVFSLYHDMKDVPNDNAGKMNLYAKTFSKVVNHNLSPFFKTWGWPIQPNTEQELSHLPAW 903

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Zebrafish
Match: zgc:162193 (zgc:162193 [Source:NCBI gene;Acc:323315])

HSP 1 Score: 61.6178 bits (148), Expect = 4.852e-11
Identity = 40/143 (27.97%), Postives = 61/143 (42.66%), Query Frame = 1

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCF 435
            W F   T E T NL+ L+ + +++       N         +  + K Y D     + W    + AL  Y Q+   FG+  FK+VF+ Y      P  ++ KMN +    S++V  NL P F  WG+PI       L   P +
Sbjct:  765 WEFPPHTTECTCNLWSLYVHEQVF--GIKSANAHPAITPADRQARTKMYFDGGKDLNSWCM--WMALETYMQLQEKFGWDAFKKVFSLYHDMTGVPNDNAGKMNLYAQTFSKVVNLNLSPFFKAWGWPIQPNTEQNLSHLPAW 903

[Close]

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCF 435
            W F   T E T NL+ L+ + +++       N         +  + K Y D     + W    + AL  Y Q+   FG+  FK+VF+ Y      P  ++ KMN +    S++V  NL P F  WG+PI       L   P +
Sbjct:  765 WEFPPHTTECTCNLWSLYVHEQVF--GIKSANAHPAITPADRQARTKMYFDGGKDLNSWCM--WMALETYMQLQEKFGWDAFKKVFSLYHDMTGVPNDNAGKMNLYAQTFSKVVNLNLSPFFKAWGWPIQPNTEQNLSHLPAW 903

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000045059.1 (TRPM8 channel-associated factor 2 [Source:NCBI gene;Acc:100170200])

HSP 1 Score: 62.3882 bits (150), Expect = 4.812e-11
Identity = 41/148 (27.70%), Postives = 72/148 (48.65%), Query Frame = 1

Query:    4 LRWTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENA---KFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCF 435
            + W F   T E T NL+ ++ +  +     N+ + S+  Q   +   +++Y+   +   ++D+WT     AL  Y Q+   FG+  FK++F  Y + +     +  KMN W    S  V  NL+P F  WG+PI+ E  + L + P +
Sbjct:  751 INWEFPPHTTEATCNLWSVYVHETVLGIPRNKAHGSL--QPETRACYIQEYVKNGSNLEQWDVWT-----ALETYLQLQEGFGWEPFKQLFKDYQSMSGISNDNKSKMNLWAEKFSEAVQTNLIPFFEAWGWPIEEETRSKLSALPVW 891

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000045101.1 (TRPM8 channel-associated factor 2 [Source:NCBI gene;Acc:100170200])

HSP 1 Score: 60.077 bits (144), Expect = 2.419e-10
Identity = 40/143 (27.97%), Postives = 66/143 (46.15%), Query Frame = 1

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCF 435
            W F   T E T NL+ +F    +     ++ N ++  +   +   ++ YL    K + W    + AL  Y Q+   FG+  FK++F  Y + +     +  KMN W    S  V  NL+P F  WG+PI+ E  + L + P +
Sbjct:  753 WEFPPHTTEATCNLWSVFVNETVVGVPRDKANSAL--EPASRAACIEAYLKTGPKLENWNV--WTALETYLQLQEGFGWEPFKQLFKDYQSMSGISNDNKSKMNLWAEKFSEAVQTNLIPFFEAWGWPIEEETRSKLSALPVW 891

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000045084.1 (TRPM8 channel-associated factor 2 [Source:NCBI gene;Acc:100170200])

HSP 1 Score: 57.3806 bits (137), Expect = 2.082e-9
Identity = 40/148 (27.03%), Postives = 69/148 (46.62%), Query Frame = 1

Query:    4 LRWTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENA---KFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCF 435
            + W F   T E T NL+ ++ +  +     N+ + S+  Q   +   +++Y+   +   ++D+WT     AL  Y Q+   FG+  FK +F  Y + +     +  KMN W    S  V  NL+P F  WG+PI+      L + P +
Sbjct:  751 INWEFPPHTTEATCNLWSVYVHETVLGIPRNKAHGSL--QPETRACYIQEYVKNGSNLEQWDVWT-----ALETYLQLQEGFGWEPFKRLFKDYQSMSGISNDNKSKMNLWAEKFSEAVQTNLIPFFEAWGWPIEEATRIKLSALPVW 891

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000002244.1 (TRPM8 channel-associated factor homolog [Source:NCBI gene;Acc:100489234])

HSP 1 Score: 56.9954 bits (136), Expect = 3.207e-9
Identity = 37/144 (25.69%), Postives = 69/144 (47.92%), Query Frame = 1

Query:    7 RWTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCF 435
            +W F   T E T NL+ ++ +  +     +  + ++  Q   +  ++K YL   A  + W  + + AL  Y Q+    G+  F+ ++   S + ++   ++ KMN W    SR    NL+P F  WG+PID+   + L + P +
Sbjct:  730 QWEFPPHTTEATCNLWAVYVHETVLGIPRDRAHGNL--QPNVRTSRIKSYLQNGANLEQW--DGWTALETYLQLQEGLGWDPFRRLYRDPSISGMS-NENTFKMNLWAEKFSRAAQTNLVPFFEAWGWPIDQSTRSKLSALPVW 868

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000045039.1 (TRPM8 channel-associated factor 2 [Source:NCBI gene;Acc:100170200])

HSP 1 Score: 56.225 bits (134), Expect = 5.069e-9
Identity = 38/135 (28.15%), Postives = 65/135 (48.15%), Query Frame = 1

Query:    4 LRWTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENA---KFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPID 396
            + W F   T E T NL+ ++ +  +     N+ + S+  Q   +   +++Y+   +   ++D+WT     AL  Y Q+   FG+  FK +F  Y + +     +  KMN W    S  V  NL+P F  WG+PI+
Sbjct:  751 INWEFPPHTTEATCNLWSVYVHETVLGIPRNKAHGSL--QPETRACYIQEYVKNGSNLEQWDVWT-----ALETYLQLQEGFGWEPFKRLFKDYQSMSGISNDNKSKMNLWAEKFSEAVQTNLIPFFEAWGWPIE 878

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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Mouse
Match: Tcaf1 (TRPM8 channel-associated factor 1 [Source:MGI Symbol;Acc:MGI:1914665])

HSP 1 Score: 67.0106 bits (162), Expect = 8.185e-13
Identity = 42/144 (29.17%), Postives = 71/144 (49.31%), Query Frame = 1

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYY---SANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFP 429
            W F   T E T NL+C++ +  +     +  N +++     + K+V+ YL +      W +  + AL  Y Q+   FG+  F  +F  Y   + ++  P  + DKMN W+ + S+ V +NL P F  WG+PI +E+   L   P
Sbjct:  768 WEFPPHTTEATCNLWCVYVHETVLGIPRSRANIALWPPV--REKRVRIYLSKGPSVKHWNA--WTALETYLQLQEAFGWEPFIRLFTEYRNQTTSSSPPSDNVDKMNLWVKMFSQQVQRNLAPFFEAWGWPIQKEVATSLAYLP 907

[Close]

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYY---SANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFP 429
            W F   T E T NL+C++ +  +     +  N +++     + K+V+ YL +      W +  + AL  Y Q+   FG+  F  +F  Y   + ++  P  + DKMN W+ + S+ V +NL P F  WG+PI +E+   L   P
Sbjct:  768 WEFPPHTTEATCNLWCVYVHETVLGIPRSRANIALWPPV--REKRVRIYLSKGPSVKHWNA--WTALETYLQLQEAFGWEPFIRLFTEYRNQTTSSSPPSDNVDKMNLWVKMFSQQVQRNLAPFFEAWGWPIQKEVATSLAYLP 907

[Close]

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYY---SANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFP 429
            W F   T E T NL+C++ +  +     +  N +++     + K+V+ YL +      W +  + AL  Y Q+   FG+  F  +F  Y   + ++  P  + DKMN W+ + S+ V +NL P F  WG+PI +E+   L   P
Sbjct:  768 WEFPPHTTEATCNLWCVYVHETVLGIPRSRANIALWPPV--REKRVRIYLSKGPSVKHWNA--WTALETYLQLQEAFGWEPFIRLFTEYRNQTTSSSPPSDNVDKMNLWVKMFSQQVQRNLAPFFEAWGWPIQKEVATSLAYLP 907

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Mouse
Match: Tcaf3 (TRPM8 channel-associated factor 3 [Source:MGI Symbol;Acc:MGI:3042585])

HSP 1 Score: 67.0106 bits (162), Expect = 9.341e-13
Identity = 43/145 (29.66%), Postives = 70/145 (48.28%), Query Frame = 1

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIW----KQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFP 429
            WTF   T E   NL+ ++ +  +     +QA+   N  +      + +++K +L++ A    W    + AL  Y Q+   FG+  F +VFA Y   +  P+ + DKMN W+   S  V +NL P F  WG+P+   +   L S P
Sbjct:  759 WTFPPHTTEALCNLWTIYVHETVLNIPREQAHPSLNPEL------RRQRIKYHLNKGAPLSNWIM--WTALETYLQLQEGFGWEPFIQVFADYRTLSGLPQNNEDKMNLWVKKFSEAVHKNLAPFFEAWGWPVKYAVAKSLASLP 895

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Mouse
Match: Tcaf2 (TRPM8 channel-associated factor 2 [Source:MGI Symbol;Acc:MGI:2385258])

HSP 1 Score: 64.6994 bits (156), Expect = 4.814e-12
Identity = 42/150 (28.00%), Postives = 74/150 (49.33%), Query Frame = 1

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIW----KQAYNEYNESIFFQNYHKLKKVKKYLDENA---KFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCF 435
            W F   T E T NL+ ++ +  +      QA+ +       +   + K++K++L + A    +++WT     AL  Y Q+   FG+  F  +FA Y      P+ +  KMN W+ + S  V +NL+P F  WG+PI +++   L  +P +
Sbjct:  769 WEFPPHTTEATCNLWSVYVHETVLGIPRAQAHPQ------LKPEEREKRIKEHLQKGAPLQNWNVWT-----ALETYLQLQEVFGWEPFITLFAEYQTIFYIPEDNECKMNIWLKLFSEKVQKNLVPFFEAWGWPIQKDVAEDLACYPSW 907

[Close]

BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
Match: sp|A6NFQ2|TCAF2_HUMAN (TRPM8 channel-associated factor 2 OS=Homo sapiens OX=9606 GN=TCAF2 PE=1 SV=2)

HSP 1 Score: 71.2478 bits (173), Expect = 2.125e-13
Identity = 42/144 (29.17%), Postives = 75/144 (52.08%), Query Frame = 1

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENA---KFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFP 429
            W F   T E T NL+ ++ +  +      + +E++      + +++K +L + A    +++WT     AL  Y Q+   FG+  F ++FA Y   +  PK ++ +MN W+   S  V +NL+P F  WG+PI +E+ + L S P
Sbjct:  768 WEFPPHTTEATCNLWSVYVHETVLGIPRAQAHEAL--SPPERERRIKAHLGKGAPLCDWNVWT-----ALETYLQLQEAFGWEPFTQLFAEYQTLSHLPKDNTGRMNLWVKKFSEKVKKNLVPFFEAWGWPIQKEVADSLASLP 904

[Close]

BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q6P6V7|TCAF3_RAT (TRPM8 channel-associated factor 3 OS=Rattus norvegicus OX=10116 GN=Tcaf3 PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 5.313e-13
Identity = 42/145 (28.97%), Postives = 70/145 (48.28%), Query Frame = 1

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIWK----QAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFP 429
            WTF+  T E   NL+ ++ +  +      QA+   N  +      + +++K +L++ A    W    + AL  Y Q+   FG+  F ++FA Y      P+ + DKMN W+   S +V +NL P F  WG+P+   +   L S P
Sbjct:  759 WTFRPHTTEALCNLWSIYVHETVLNIPRDQAHPSLNPEL------RKQRIKDHLNKGAPLSNWIV--WTALETYLQLQEGFGWEPFIQLFANYQTLTGLPQNNEDKMNLWVKKFSEVVQKNLAPFFKAWGWPVQHAVAKSLASLP 895

[Close]

BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q8BNE1|TCAF1_MOUSE (TRPM8 channel-associated factor 1 OS=Mus musculus OX=10090 GN=Tcaf1 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 5.727e-12
Identity = 42/144 (29.17%), Postives = 71/144 (49.31%), Query Frame = 1

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYY---SANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFP 429
            W F   T E T NL+C++ +  +     +  N +++     + K+V+ YL +      W +  + AL  Y Q+   FG+  F  +F  Y   + ++  P  + DKMN W+ + S+ V +NL P F  WG+PI +E+   L   P
Sbjct:  768 WEFPPHTTEATCNLWCVYVHETVLGIPRSRANIALWPPV--REKRVRIYLSKGPSVKHWNA--WTALETYLQLQEAFGWEPFIRLFTEYRNQTTSSSPPSDNVDKMNLWVKMFSQQVQRNLAPFFEAWGWPIQKEVATSLAYLP 907

[Close]

BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q6QR59|TCAF3_MOUSE (TRPM8 channel-associated factor 3 OS=Mus musculus OX=10090 GN=Tcaf3 PE=1 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 6.534e-12
Identity = 43/145 (29.66%), Postives = 70/145 (48.28%), Query Frame = 1

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIW----KQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFP 429
            WTF   T E   NL+ ++ +  +     +QA+   N  +      + +++K +L++ A    W    + AL  Y Q+   FG+  F +VFA Y   +  P+ + DKMN W+   S  V +NL P F  WG+P+   +   L S P
Sbjct:  759 WTFPPHTTEALCNLWTIYVHETVLNIPREQAHPSLNPEL------RRQRIKYHLNKGAPLSNWIM--WTALETYLQLQEGFGWEPFIQVFADYRTLSGLPQNNEDKMNLWVKKFSEAVHKNLAPFFEAWGWPVKYAVAKSLASLP 895

[Close]

BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9Y4C2|TCAF1_HUMAN (TRPM8 channel-associated factor 1 OS=Homo sapiens OX=9606 GN=TCAF1 PE=1 SV=3)

HSP 1 Score: 66.6254 bits (161), Expect = 7.693e-12
Identity = 41/141 (29.08%), Postives = 66/141 (46.81%), Query Frame = 1

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFP 429
            W F   T E T NL+C++ +  +     +  N +++     + K+V+ YL +      W +  + AL  Y Q+   FG+  F  +F  Y      P  + DKMN W+ + S  V +NL P F  W +PI +E+   L   P
Sbjct:  768 WEFPPHTTEATCNLWCVYVHETVLGIPRSRANIALWPPV--REKRVRIYLSKGPNVKNWNA--WTALETYLQLQEAFGWEPFIRLFTEYRNQTNLPTENVDKMNLWVKMFSHQVQKNLAPFFEAWAWPIQKEVATSLAYLP 904

[Close]

BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Match: A0A1S6KMG4 (Peptidase M60 domain-containing protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 9.695e-42
Identity = 81/179 (45.25%), Postives = 111/179 (62.01%), Query Frame = 1

Query:    7 RWTFKGTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCFFPY-DYYVNISTIRIKIVETRYKSCQKKAKYYKDIS 540
            +WTF GT EVTNNL CLF Y K++K      + S    +      +  YL+  A F  WTS P+ ALLIY+QM+ + G+G +K++F YY  NN++P TDS+KM T+MN+TS I  QN++ IF FWGFPID  +   L + PCFFP  D+ V +S  R   +  +Y  C  K+ +YKD S
Sbjct:  730 KWTFYGTTEVTNNLQCLFVYAKLFKIPRYGTDLSSLGSD---TSTISAYLEGGAVFSQWTSNPWLALLIYSQMSQYLGWGFYKDLFEYYDTNNISPSTDSEKMITFMNITSIISQQNMISIFDFWGFPIDNNLSQQLSTLPCFFPADDFLVALSPKRKNYISNKYPQCNSKSSFYKDYS 905

[Close]

BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Match: A0A1S6KMG0 (Peptidase M60 domain-containing protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 2.307e-36
Identity = 73/187 (39.04%), Postives = 100/187 (53.48%), Query Frame = 1

Query:   10 WTFKGTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYY-SANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCFFPYDYYVNISTIRIKIVETRYKSCQKKAKYYKDISEGTLREEKR 567
            WT     E TNN+FCLFN  K WK  Y    + +   NY +   +K YLD  A   +W    +  L IY QMANHFGFG F + F YY +   L   +D +K + W NVTS I  QNL+PIF  W  PI  E+   L  F CF P D+++  S  R + +  +Y  C +K+ +Y+D +  +L +  R
Sbjct:  740 WTMSTVSEATNNIFCLFNTAKEWKLPYYGSTKELLDGNYDE---IKSYLDGGADLAVWKESAYLELAIYVQMANHFGFGFFNKAFKYYLNHPELKADSDEEKYDLWANVTSTITNQNLMPIFDLWNIPIGDEMRKYLSKFQCFLPDDFFLKSSRSREEFILKKYPDCHRKSTFYEDFNPESLTQGHR 923

[Close]

BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Match: A0A4Q3UK88 (Peptidase M60 domain-containing protein (Fragment) OS=bacterium OX=1869227 GN=EON82_04960 PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 4.721e-25
Identity = 55/145 (37.93%), Postives = 77/145 (53.10%), Query Frame = 1

Query:   10 WTFKGTVEVTNNLFCLFNYNKIWKQAYNEYNES-IFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCFFP 441
            WTF GT EVTNNLF L+   K+      EY  +        + K+++ YL + AKF+ W S+PF AL +YAQ+   FG+  F + FA Y  + + P+ +  + + WM   SR  G+NL P F  WG P        LES P + P
Sbjct:   61 WTFDGTGEVTNNLFSLYASEKLNGITPAEYGVAHPAMAPEAQKKRLEAYLAKGAKFEDWKSDPFLALTMYAQLREAFGWEPFTKAFAAYRRDGIRPQGELAQHDAWMVQMSRATGRNLGPFFQAWGVPTSESARKSLESLPGWMP 205

[Close]

BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Match: R7UHN9 (Peptidase M60 domain-containing protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_195748 PE=4 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 2.705e-24
Identity = 58/150 (38.67%), Postives = 79/150 (52.67%), Query Frame = 1

Query:    4 LRWTFKGTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYS--ANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCFFPYD 447
            L WTF+GT EVT N+F L   + +     N     I     +++  +K+YL   A F  W  EP  AL IYAQ+A+H G+G +K VF  Y   +N   PKTD +K+  W+   +   GQNL  +F FWGF I  ++   L   P F P D
Sbjct:  532 LEWTFQGTSEVTVNVFTLHAMDVV-----NGVKPWIHPWLNNQMNAIKRYLMAGADFTTWKKEPGIALGIYAQLAHHLGWGTYKAVFRKYEVMSNEQKPKTDDEKIRLWITTFAECCGQNLCALFRFWGFTIPNDLQRNLAELPLFLPMD 676

[Close]

BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Match: V4BHI6 (Peptidase M60 domain-containing protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_126693 PE=4 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 5.138e-24
Identity = 59/159 (37.11%), Postives = 87/159 (54.72%), Query Frame = 1

Query:   10 WTFKGTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLN--PKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCFFPYDYYVNISTIRIK 480
            WTFKGT  VTN++F L+ Y+ + +         +  +   ++K VK+YLD +  F  W +EP  AL IYAQ+ANHFG+GL+K +F  Y   N +  P  +  K++ W    S I G NL P+  FW  P+   + N L  FP F P D    ++  R++
Sbjct:  235 WTFKGTENVTNDIFTLYVYDIVCR-----IGPWMPERLLSRIKYVKRYLDRDEGFFEWLTEPSLALFIYAQLANHFGWGLYKSIFRKYRLMNQSAIPHHNVTKIDFWFTSLSEIAGYNLAPLCDFWRIPLSTAVRNHLTQFPPFLPDDEVTRLAPQRVR 388

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Cavefish
Match: si:ch211-210b2.3 (si:ch211-210b2.3 [Source:ZFIN;Acc:ZDB-GENE-131122-13])

HSP 1 Score: 66.6254 bits (161), Expect = 9.459e-13
Identity = 42/147 (28.57%), Postives = 75/147 (51.02%), Query Frame = 1

Query:    7 RWTFK---GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVF-AYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCF 435
            RW ++    T E T NL+ ++ + ++        +E++  +   +  + + Y     + D W+   + AL  Y Q+ + FG+  FK+VF AY++ NN+ P  ++ KMN +    S++V  NL P F  WG+PI     + L S P +
Sbjct:  744 RWAWEFPPNTTECTCNLWSVYVHEEVLGVNRASAHENMSPE--RRWGRAQSYAMGGRQLDQWSM--WTALETYMQLQDRFGWDAFKKVFTAYHTINNV-PGDNNGKMNLYAETFSKVVNMNLAPFFKAWGWPIQPSTEDALSSLPVW 885

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000051129.1 (pep primary_assembly:Astyanax_mexicanus-2.0:1:5658439:5662219:-1 gene:ENSAMXG00000036822.1 transcript:ENSAMXT00000051129.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 63.1586 bits (152), Expect = 3.978e-12
Identity = 42/141 (29.79%), Postives = 66/141 (46.81%), Query Frame = 1

Query:   10 WTFKG-TVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFP 429
            W F   T E T NL+ ++ +  +      + +  +  Q   +  ++KKY++       WT   + AL  Y Q+   FG+  FK+VFA Y      P+ +  KMN +    S++V +NL P F  WG+PI       L S P
Sbjct:   74 WEFPPYTTECTCNLWSVYVHEVVLGVKKADAHGQMTPQR--RQNRIKKYIEGGRNLKDWTV--WTALETYMQLQEEFGWDAFKKVFAAYHDMTGVPQDNKGKMNLYAETFSKVVNRNLTPFFKAWGWPIQPSTEEQLCSLP 210

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000976.1 (pep scaffold:Pmarinus_7.0:GL479898:35358:38300:-1 gene:ENSPMAG00000000885.1 transcript:ENSPMAT00000000976.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 61.6178 bits (148), Expect = 9.931e-12
Identity = 37/127 (29.13%), Postives = 58/127 (45.67%), Query Frame = 1

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGF 387
            W F   T E TNNLF ++ + ++   A    +  +  +   + ++++ ++D       WT   F  L  Y Q+   FG+  F  +F  Y      P T   KM+ W    SR VG+NL P F  WG+
Sbjct:  754 WNFPPHTTEATNNLFAVYVHEEVLGVARARAHPDL--RPAERERRLRDHVDAGCPLAAWTV--FTCLETYLQLQEAFGWDPFVRLFGDYQRAASTPSTMEAKMSEWAERFSRCVGRNLAPFFSAWGW 876

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Medaka
Match: si:ch211-210b2.3 (TRPM8 channel-associated factor homolog [Source:NCBI gene;Acc:101159605])

HSP 1 Score: 60.8474 bits (146), Expect = 7.952e-11
Identity = 38/143 (26.57%), Postives = 68/143 (47.55%), Query Frame = 1

Query:   10 WTFK-GTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCF 435
            W F   T E T NL+ ++ + ++      + + ++  +N  +  + + Y+ +    + W  E + AL  Y Q+ + FG+  FK+VFA Y   +  P  +  KMN +    S+ VG +L   F  WG+PI       L + P +
Sbjct:  769 WEFPPNTTECTCNLWSVYVHEEVLGIDRAKAHPNMSLEN--RKSRARNYVTQGRNLNSW--EMWVALETYMQLQDKFGWDAFKKVFAAYLQMSQFPGDNPGKMNLYTETFSQTVGMDLTAFFKAWGWPIQASTEQKLSNLPSW 907

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000011627.1 (SMESG000011627.1)

HSP 1 Score: 390.193 bits (1001), Expect = 4.701e-131
Identity = 186/189 (98.41%), Postives = 187/189 (98.94%), Query Frame = 1

Query:    1 NLRWTFKGTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCFFPYDYYVNISTIRIKIVETRYKSCQKKAKYYKDISEGTLREEKR 567
            NLRWTFKGTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFG FKEVFAYYSANNLNPKT+SDKM TWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCFFPYDYYVNISTIRIKIVETRYKSCQKKAKYYKDISEGTLREEKR
Sbjct:  731 NLRWTFKGTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGFFKEVFAYYSANNLNPKTNSDKMKTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCFFPYDYYVNISTIRIKIVETRYKSCQKKAKYYKDISEGTLREEKR 919

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000011622.1 (SMESG000011622.1)

HSP 1 Score: 274.633 bits (701), Expect = 4.057e-87
Identity = 131/187 (70.05%), Postives = 147/187 (78.61%), Query Frame = 1

Query:    7 RWTFKGTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCFFPYDYYVNISTIRIKIVETRYKSCQKKAKYYKDISEGTLREEKR 567
            +WTF GT EVTNNLFCLFNYNK+WK  Y    E   +++Y ++K +KKYLDENA FDIWTSEPFHALLIYAQMANHFGF  FKEVFAYYS NNL PKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPID EI   ++S  C FP D+Y  IS IR K+VE +YKSC   +KY+ D SE  LR + R
Sbjct:  730 QWTFHGTTEVTNNLFCLFNYNKVWKLPYYGSEELKLYRDYDRIKDIKKYLDENANFDIWTSEPFHALLIYAQMANHFGFEFFKEVFAYYSDNNLEPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDSEIEVSMKSLRCLFPEDFYREISEIRTKVVEAKYKSCDFNSKYFSDFSEENLRFDIR 916

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000042720.1 (SMESG000042720.1)

HSP 1 Score: 160.614 bits (405), Expect = 1.351e-45
Identity = 82/178 (46.07%), Postives = 110/178 (61.80%), Query Frame = 1

Query:   10 WTFKGTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCFFPY-DYYVNISTIRIKIVETRYKSCQKKAKYYKDIS 540
            WTF GT EVTNNL CLF Y K++K      + S    +      +  YL+  A F  WTS P+ ALLIY+QM+ + G+G +K+VF YY  NN++P TDS+KM T+MN+TS I  QN++ IF FWGFPID  +   L + PCFFP  D+ V +S  R   +  +Y  C  K+ +YKD S
Sbjct:  669 WTFYGTTEVTNNLQCLFVYAKLFKIPRYGTDLSSLGSD---TTSISAYLEGGAVFSQWTSNPWLALLIYSQMSQYLGWGFYKDVFEYYDTNNISPSTDSEKMITFMNITSIISQQNMISIFDFWGFPIDNNLSQQLSTLPCFFPADDFLVALSPKRKNYISNKYPQCNSKSSFYKDYS 843

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000042174.1 (SMESG000042174.1)

HSP 1 Score: 160.614 bits (405), Expect = 1.530e-45
Identity = 81/177 (45.76%), Postives = 111/177 (62.71%), Query Frame = 1

Query:    7 RWTFKGTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCFFPY-DYYVNISTIRIKIVETRYKSCQKKAKYYKD 534
            +WTF+GT EVTNNL CLF + K +K     Y   I      K  K+  YL+  A+F  W S+P+ ALLIY+QM+ + G+G FK+VF +Y  N ++P TDS KM T+MN++S I  QNL+ +F FWGFPID  +   L   PCFFP  D+ V++S IR   +  +Y  C  K+ +YKD
Sbjct:  728 KWTFQGTTEVTNNLLCLFVFEKFFKLP--RYGSDIDHIRNDK-SKITAYLEGGAEFSKWRSDPWLALLIYSQMSQYLGWGFFKDVFEFYETNKISPITDSQKMITFMNISSIISQQNLISMFDFWGFPIDESLRQQLSKLPCFFPADDFLVSLSLIRKNYISNKYPECNSKSSFYKD 901

[Close]

BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000042174.1 (SMESG000042174.1)

HSP 1 Score: 160.614 bits (405), Expect = 1.550e-45
Identity = 81/177 (45.76%), Postives = 111/177 (62.71%), Query Frame = 1

Query:    7 RWTFKGTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKKYLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKTDSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCFFPY-DYYVNISTIRIKIVETRYKSCQKKAKYYKD 534
            +WTF+GT EVTNNL CLF + K +K     Y   I      K  K+  YL+  A+F  W S+P+ ALLIY+QM+ + G+G FK+VF +Y  N ++P TDS KM T+MN++S I  QNL+ +F FWGFPID  +   L   PCFFP  D+ V++S IR   +  +Y  C  K+ +YKD
Sbjct:  731 KWTFQGTTEVTNNLLCLFVFEKFFKLP--RYGSDIDHIRNDK-SKITAYLEGGAEFSKWRSDPWLALLIYSQMSQYLGWGFFKDVFEFYETNKISPITDSQKMITFMNISSIISQQNLISMFDFWGFPIDESLRQQLSKLPCFFPADDFLVSLSLIRKNYISNKYPECNSKSSFYKD 904

The following BLAST results are available for this feature:

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Human
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Celegans
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Fly
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Zebrafish
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Mouse
BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Cavefish
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Sea Lamprey
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Yeast
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Nematostella
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Medaka
BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
TCAF2C	3.883e-14	29.17	TRPM8 channel associated factor 2C [Source:HGNC Sy...	[more]
TCAF2	4.341e-14	29.17	TRPM8 channel associated factor 2 [Source:HGNC Sym...	[more]
TCAF2	4.341e-14	29.17	TRPM8 channel associated factor 2 [Source:HGNC Sym...	[more]
TCAF2	4.426e-14	29.17	TRPM8 channel associated factor 2 [Source:HGNC Sym...	[more]
TCAF1	1.601e-12	29.08	TRPM8 channel associated factor 1 [Source:HGNC Sym...	[more]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
si:ch211-210b2.3	9.428e-13	27.97	si:ch211-210b2.3 [Source:ZFIN;Acc:ZDB-GENE-131122-...	[more]
si:ch211-210b2.2	2.639e-11	27.97	si:ch211-210b2.2 [Source:ZFIN;Acc:ZDB-GENE-131118-...	[more]
si:ch211-210b2.4	4.050e-11	27.97	si:ch211-210b2.4 [Source:ZFIN;Acc:ZDB-GENE-131122-...	[more]
zgc:162193	4.852e-11	27.97	zgc:162193 [Source:NCBI gene;Acc:323315]	[more]
zgc:162193	4.852e-11	27.97	zgc:162193 [Source:NCBI gene;Acc:323315]	[more]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSXETT00000045059.1	4.812e-11	27.70	TRPM8 channel-associated factor 2 [Source:NCBI gen...	[more]
ENSXETT00000045101.1	2.419e-10	27.97	TRPM8 channel-associated factor 2 [Source:NCBI gen...	[more]
ENSXETT00000045084.1	2.082e-9	27.03	TRPM8 channel-associated factor 2 [Source:NCBI gen...	[more]
ENSXETT00000002244.1	3.207e-9	25.69	TRPM8 channel-associated factor homolog [Source:NC...	[more]
ENSXETT00000045039.1	5.069e-9	28.15	TRPM8 channel-associated factor 2 [Source:NCBI gen...	[more]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Tcaf1	8.185e-13	29.17	TRPM8 channel-associated factor 1 [Source:MGI Symb...	[more]
Tcaf1	8.185e-13	29.17	TRPM8 channel-associated factor 1 [Source:MGI Symb...	[more]
Tcaf1	8.185e-13	29.17	TRPM8 channel-associated factor 1 [Source:MGI Symb...	[more]
Tcaf3	9.341e-13	29.66	TRPM8 channel-associated factor 3 [Source:MGI Symb...	[more]
Tcaf2	4.814e-12	28.00	TRPM8 channel-associated factor 2 [Source:MGI Symb...	[more]

BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|A6NFQ2\|TCAF2_HUMAN	2.125e-13	29.17	TRPM8 channel-associated factor 2 OS=Homo sapiens ...	[more]
sp\|Q6P6V7\|TCAF3_RAT	5.313e-13	28.97	TRPM8 channel-associated factor 3 OS=Rattus norveg...	[more]
sp\|Q8BNE1\|TCAF1_MOUSE	5.727e-12	29.17	TRPM8 channel-associated factor 1 OS=Mus musculus ...	[more]
sp\|Q6QR59\|TCAF3_MOUSE	6.534e-12	29.66	TRPM8 channel-associated factor 3 OS=Mus musculus ...	[more]
sp\|Q9Y4C2\|TCAF1_HUMAN	7.693e-12	29.08	TRPM8 channel-associated factor 1 OS=Homo sapiens ...	[more]

BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A1S6KMG4	9.695e-42	45.25	Peptidase M60 domain-containing protein OS=Schmidt...	[more]
A0A1S6KMG0	2.307e-36	39.04	Peptidase M60 domain-containing protein OS=Schmidt...	[more]
A0A4Q3UK88	4.721e-25	37.93	Peptidase M60 domain-containing protein (Fragment)...	[more]
R7UHN9	2.705e-24	38.67	Peptidase M60 domain-containing protein OS=Capitel...	[more]
V4BHI6	5.138e-24	37.11	Peptidase M60 domain-containing protein OS=Lottia ...	[more]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
si:ch211-210b2.3	9.459e-13	28.57	si:ch211-210b2.3 [Source:ZFIN;Acc:ZDB-GENE-131122-...	[more]
ENSAMXT00000051129.1	3.978e-12	29.79	pep primary_assembly:Astyanax_mexicanus-2.0:1:5658...	[more]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSPMAT00000000976.1	9.931e-12	29.13	pep scaffold:Pmarinus_7.0:GL479898:35358:38300:-1 ...	[more]

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Peptidase M60 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
si:ch211-210b2.3	7.952e-11	26.57	TRPM8 channel-associated factor homolog [Source:NC...	[more]

BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000011627.1	4.701e-131	98.41	SMESG000011627.1	[more]
SMESG000011622.1	4.057e-87	70.05	SMESG000011622.1	[more]
SMESG000042720.1	1.351e-45	46.07	SMESG000042720.1	[more]
SMESG000042174.1	1.530e-45	45.76	SMESG000042174.1	[more]
SMESG000042174.1	1.550e-45	45.76	SMESG000042174.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30012259 ID=SMED30012259|Name=Peptidase M60 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=569bp
AATCTAAGGTGGACCTTCAAAGGTACAGTAGAAGTAACAAATAATTTATT
TTGCTTGTTCAATTACAATAAAATATGGAAGCAGGCATATAATGAATACA
ATGAATCCATATTTTTTCAAAATTATCACAAATTAAAAAAGGTTAAAAAG
TATTTAGATGAAAATGCTAAATTTGATATTTGGACTTCTGAACCCTTTCA
CGCTCTTCTTATCTATGCTCAAATGGCGAATCATTTCGGTTTTGGGCTCT
TCAAGGAAGTCTTTGCATATTATTCGGCCAACAATCTAAATCCCAAAACC
GATTCGGATAAAATGAATACTTGGATGAATGTAACAAGTAGAATAGTTGG
ACAGAATTTGCTGCCGATATTTTATTTCTGGGGCTTCCCCATTGACAGGG
AAATTGGAAATATATTGGAAAGTTTTCCCTGCTTTTTCCCATACGATTAT
TATGTAAATATTAGTACTATTCGAATTAAAATAGTGGAAACTAGATATAA
ATCCTGTCAGAAAAAAGCCAAATATTATAAGGACATTTCAGAAGGAACCC
TTCGGGAGGAAAAAAGAGA

protein sequence of SMED30012259-orf-1

>SMED30012259-orf-1 ID=SMED30012259-orf-1|Name=SMED30012259-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=189bp
NLRWTFKGTVEVTNNLFCLFNYNKIWKQAYNEYNESIFFQNYHKLKKVKK
YLDENAKFDIWTSEPFHALLIYAQMANHFGFGLFKEVFAYYSANNLNPKT
DSDKMNTWMNVTSRIVGQNLLPIFYFWGFPIDREIGNILESFPCFFPYDY
YVNISTIRIKIVETRYKSCQKKAKYYKDISEGTLREEKR

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **INTERPRO**
Term	Definition
IPR042279	Pep_M60_3
IPR031161	Peptidase_M60_dom

Vocabulary: **cellular component**
Term	Definition
GO:0005886	plasma membrane
GO:0016020	membrane

Vocabulary: **biological process**
Term	Definition
GO:0006810	transport
GO:0010360	negative regulation of anion channel activity
GO:0030335	positive regulation of cell migration
GO:0090314	positive regulation of protein targeting to membrane

Vocabulary: **molecular function**
Term	Definition
GO:0044325	ion channel binding

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR031161	Peptidase family M60 domain	PFAM	PF13402	Peptidase_M60	coord: 3..128 e-value: 2.1E-14 score: 53.7
IPR031161	Peptidase family M60 domain	PROSITE	PS51723	PEPTIDASE_M60	coord: 1..80 score: 16.826
IPR042279	Peptidase M60, enhancin-like domain 3	GENE3D	G3DSA:1.10.390.30		coord: 1..146 e-value: 1.8E-22 score: 81.9
None	No IPR available	PANTHER	PTHR15730	EXPERIMENTAL AUTOIMMUNE PROSTATITIS ANTIGEN 2-RELATED	coord: 4..145
None	No IPR available	PANTHER	PTHR15730:SF4	TRPM8 CHANNEL-ASSOCIATED FACTOR 2	coord: 4..145

Main menu

Peptidase M60 domain-containing protein

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 9

Owlery

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