Transcription initiation factor IIB

Overview
NameTranscription initiation factor IIB
Smed IDSMED30012239
Length (bp)1163
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Transcription initiation factor IIB (SMED30012239) t-SNE clustered cells

Violin plots show distribution of expression levels for Transcription initiation factor IIB (SMED30012239) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Transcription initiation factor IIB (SMED30012239) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Transcription initiation factor IIB (SMED30012239) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012239

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30012239SMESG000077013.1 SmedASXL_011922SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
zeta neoblastSMED30012239SMESG000077013.1 dd_Smed_v4_15237_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30012239SMESG000077013.1 SMED_08996_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30012239SMESG000077013.1 SMED_08996_V2_1GPL14150PMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Transcription initiation factor IIB vs. Ensembl Human
Match: GTF2B (general transcription factor IIB [Source:HGNC Symbol;Acc:HGNC:4648])

HSP 1 Score: 209.92 bits (533), Expect = 1.451e-64
Identity = 123/306 (40.20%), Postives = 172/306 (56.21%), Query Frame = 3 
Query:  108 QIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            ++ C  HP+A L+ED+ AGD+ICP CGLVVGDRVID  SEWR+F+ND   +D SRVG  +N   S+   S  I         DE GN KY+N   MS  +R +      I    +R+N   N  +    L K++ E      +   AI+ ACL++A R   VPRTFKE+CAVS +SKK+IG+  K ++ A+     +   G ++SRFCS L    K V   A+ IA  A    ++  +SP ++ A +IYMAS    E+R++K IGDI GV+  TI  +Y++ Y     L P     KF   V++LP
Sbjct:   12 RVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLP-KQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDF--KFDTPVDKLP 314
[Close]
BLAST of Transcription initiation factor IIB vs. Ensembl Human
Match: GTF2B (general transcription factor IIB [Source:HGNC Symbol;Acc:HGNC:4648])

HSP 1 Score: 166.777 bits (421), Expect = 5.021e-49
Identity = 85/206 (41.26%), Postives = 118/206 (57.28%), Query Frame = 3 
Query:  108 QIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSR 725
            ++ C  HP+A L+ED+ AGD+ICP CGLVVGDRVID  SEWR+F+ND   +D SRVG  +N   S+   S  I         DE GN KY+N   MS  +R +      I    +R+N   N  +    L K++ E      +   AI+ ACL++A R   VPRTFKE+CAVS +SKK+IG+  K ++ A+     +   G ++SR
Sbjct:   11 RVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSR 216
[Close]
BLAST of Transcription initiation factor IIB vs. Ensembl Human
Match: GTF2B (general transcription factor IIB [Source:HGNC Symbol;Acc:HGNC:4648])

HSP 1 Score: 160.999 bits (406), Expect = 9.075e-47
Identity = 89/226 (39.38%), Postives = 123/226 (54.42%), Query Frame = 3 
Query:  108 QIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIA 785
            ++ C  HP+A L+ED+ AGD+ICP C     DRVID  SEWR+F+ND   +D SRVG  +N   S+   S  I         DE GN KY+N   MS  +R +      I    +R+N   N  +    L K++ E      +   AI+ ACL++A R   VPRTFKE+CAVS +SKK+IG+  K ++ A+     +   G ++SRFCS L    K V   A+ IA
Sbjct:   12 RVTCPNHPDAILVEDYRAGDMICPEC-----DRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLP-KQVQMAATHIA 231
[Close]
BLAST of Transcription initiation factor IIB vs. Ensembl Celegans
Match: ttb-1 (Transcription initiation factor IIB [Source:UniProtKB/Swiss-Prot;Acc:O16991])

HSP 1 Score: 194.512 bits (493), Expect = 3.659e-59
Identity = 117/312 (37.50%), Postives = 174/312 (55.77%), Query Frame = 3 
Query:  102 SNQIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVEN----ANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            S  + C  HP+ +LIED  AGDL+CP CGLVVGDR++D  +EWRSF+N+ +G D SRVGA EN      +   T ++G   S     L  N  +K  N+      +R +    + I E  ER++   N ++ A  + K + ES     KN  A + ACL++A R + VPRTFKE+CAVS VSKK+IG+  K ++ ++           ++SRFC  L+  N  +   A+ IA  A +  +++ ++P +I A +IYMAS    E+RS K IGD+ G ++ T+  TYK+ Y     L P     +F   ++ LP
Sbjct:    2 SAPVQCPIHPDVHLIEDHRAGDLVCPACGLVVGDRLVDVGTEWRSFSNERSGNDPSRVGAPENPLLSGGDLSTTIAVGFGGSDSDNSL-ANAQRKSMNNT-----DRQMTAAMSLIREMSERIHLPRNIQDSASRIFKDVLESKALRGKNNEAQAAACLYIACRKDGVPRTFKEICAVSRVSKKEIGRCFKIIVRSLETNLEQITSADFMSRFCGNLSLPNS-IQAAATRIAKCAVDMDLVAGRTPISIAAAAIYMASQASAEKRSAKEIGDVAGAAEITVRQTYKLLYPKALELFPKDF--RFVTPIDALP 304
[Close]
BLAST of Transcription initiation factor IIB vs. Ensembl Celegans
Match: ttb-1 (Transcription initiation factor IIB [Source:UniProtKB/Swiss-Prot;Acc:O16991])

HSP 1 Score: 194.512 bits (493), Expect = 3.659e-59
Identity = 117/312 (37.50%), Postives = 174/312 (55.77%), Query Frame = 3 
Query:  102 SNQIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVEN----ANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            S  + C  HP+ +LIED  AGDL+CP CGLVVGDR++D  +EWRSF+N+ +G D SRVGA EN      +   T ++G   S     L  N  +K  N+      +R +    + I E  ER++   N ++ A  + K + ES     KN  A + ACL++A R + VPRTFKE+CAVS VSKK+IG+  K ++ ++           ++SRFC  L+  N  +   A+ IA  A +  +++ ++P +I A +IYMAS    E+RS K IGD+ G ++ T+  TYK+ Y     L P     +F   ++ LP
Sbjct:    2 SAPVQCPIHPDVHLIEDHRAGDLVCPACGLVVGDRLVDVGTEWRSFSNERSGNDPSRVGAPENPLLSGGDLSTTIAVGFGGSDSDNSL-ANAQRKSMNNT-----DRQMTAAMSLIREMSERIHLPRNIQDSASRIFKDVLESKALRGKNNEAQAAACLYIACRKDGVPRTFKEICAVSRVSKKEIGRCFKIIVRSLETNLEQITSADFMSRFCGNLSLPNS-IQAAATRIAKCAVDMDLVAGRTPISIAAAAIYMASQASAEKRSAKEIGDVAGAAEITVRQTYKLLYPKALELFPKDF--RFVTPIDALP 304
[Close]
BLAST of Transcription initiation factor IIB vs. Ensembl Celegans
Match: ttb-1 (Transcription initiation factor IIB [Source:UniProtKB/Swiss-Prot;Acc:O16991])

HSP 1 Score: 194.512 bits (493), Expect = 3.659e-59
Identity = 117/312 (37.50%), Postives = 174/312 (55.77%), Query Frame = 3 
Query:  102 SNQIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVEN----ANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            S  + C  HP+ +LIED  AGDL+CP CGLVVGDR++D  +EWRSF+N+ +G D SRVGA EN      +   T ++G   S     L  N  +K  N+      +R +    + I E  ER++   N ++ A  + K + ES     KN  A + ACL++A R + VPRTFKE+CAVS VSKK+IG+  K ++ ++           ++SRFC  L+  N  +   A+ IA  A +  +++ ++P +I A +IYMAS    E+RS K IGD+ G ++ T+  TYK+ Y     L P     +F   ++ LP
Sbjct:    2 SAPVQCPIHPDVHLIEDHRAGDLVCPACGLVVGDRLVDVGTEWRSFSNERSGNDPSRVGAPENPLLSGGDLSTTIAVGFGGSDSDNSL-ANAQRKSMNNT-----DRQMTAAMSLIREMSERIHLPRNIQDSASRIFKDVLESKALRGKNNEAQAAACLYIACRKDGVPRTFKEICAVSRVSKKEIGRCFKIIVRSLETNLEQITSADFMSRFCGNLSLPNS-IQAAATRIAKCAVDMDLVAGRTPISIAAAAIYMASQASAEKRSAKEIGDVAGAAEITVRQTYKLLYPKALELFPKDF--RFVTPIDALP 304
[Close]
BLAST of Transcription initiation factor IIB vs. Ensembl Fly
Match: TfIIB (gene:FBgn0004915 transcript:FBtr0080025)

HSP 1 Score: 195.667 bits (496), Expect = 2.012e-59
Identity = 119/310 (38.39%), Postives = 170/310 (54.84%), Query Frame = 3 
Query:   96 MKSNQIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            + +N++ C  HP + LIED+ AGD+IC  CGLVVGDRVID  SEWR+F+N+ +G D SRVG  EN   S    S  I         D  G  KY+N   MS  +R L      I+   +R+N      + A  L K++ +      ++  A + ACL++A R   VPRTFKE+CAVS +SKK+IG+  K  + A+     +     ++ RFC+ L+  N  V   A+ IA  A    I+  +SP ++ A +IYMAS     +RS+K IGDI GV+  TI  +YK+ Y +   L P     KF   +++LP
Sbjct:    7 LDNNKVCCYAHPESPLIEDYRAGDMICSECGLVVGDRVIDVGSEWRTFSNEKSGVDPSRVGGPENPLLSGGDLSTIIGPGTGSASFDAFGAPKYQNRRTMSSSDRSLISAFKEISSMADRINLPKTIVDRANNLFKQVHDGKNLKGRSNDAKASACLYIACRQEGVPRTFKEICAVSKISKKEIGRCFKLTLKALETSVDLITTADFMCRFCANLDLPN-MVQRAATHIAKKAVEMDIVPGRSPISVAAAAIYMASQASEHKRSQKEIGDIAGVADVTIRQSYKLMYPHAAKLFPEDF--KFTTPIDQLP 313
[Close]
BLAST of Transcription initiation factor IIB vs. Ensembl Fly
Match: TfIIB (gene:FBgn0004915 transcript:FBtr0333489)

HSP 1 Score: 195.667 bits (496), Expect = 2.012e-59
Identity = 119/310 (38.39%), Postives = 170/310 (54.84%), Query Frame = 3 
Query:   96 MKSNQIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            + +N++ C  HP + LIED+ AGD+IC  CGLVVGDRVID  SEWR+F+N+ +G D SRVG  EN   S    S  I         D  G  KY+N   MS  +R L      I+   +R+N      + A  L K++ +      ++  A + ACL++A R   VPRTFKE+CAVS +SKK+IG+  K  + A+     +     ++ RFC+ L+  N  V   A+ IA  A    I+  +SP ++ A +IYMAS     +RS+K IGDI GV+  TI  +YK+ Y +   L P     KF   +++LP
Sbjct:    7 LDNNKVCCYAHPESPLIEDYRAGDMICSECGLVVGDRVIDVGSEWRTFSNEKSGVDPSRVGGPENPLLSGGDLSTIIGPGTGSASFDAFGAPKYQNRRTMSSSDRSLISAFKEISSMADRINLPKTIVDRANNLFKQVHDGKNLKGRSNDAKASACLYIACRQEGVPRTFKEICAVSKISKKEIGRCFKLTLKALETSVDLITTADFMCRFCANLDLPN-MVQRAATHIAKKAVEMDIVPGRSPISVAAAAIYMASQASEHKRSQKEIGDIAGVADVTIRQSYKLMYPHAAKLFPEDF--KFTTPIDQLP 313
[Close]
BLAST of Transcription initiation factor IIB vs. Ensembl Zebrafish
Match: gtf2b (general transcription factor IIB [Source:ZFIN;Acc:ZDB-GENE-030131-3544])

HSP 1 Score: 205.682 bits (522), Expect = 4.429e-63
Identity = 122/308 (39.61%), Postives = 174/308 (56.49%), Query Frame = 3 
Query:  108 QIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENA--NNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            ++ C  HP+A L+ED+ AGD+ICP CGLVVGDRVID  SEWR+F+N+   +D SRVG  +N   N  + TT I           DE GN KY+N   MS  +R +      I    +R+N   N  +    L K++ E      ++  AI+ ACL++A R   VPRTFKE+CAVS +SKK+IG+  K ++ A+     +   G ++SRFCS L    K V   A+ IA  A    ++  +SP ++ A +IYMAS    E++++K IGDI GV+  TI  +Y++ Y     L P     KF   V++LP
Sbjct:   12 KVQCPNHPDALLVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNEKATKDPSRVGDAQNPLLNGGDLTTMIS--KGTGAASFDEFGNSKYQNRRTMSSSDRAMLNAFKEITTMADRINLPRNIIDRTNNLFKQVYEQKSLKGRSNDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLGLP-KQVQMAATYIARKAVELDLVPGRSPISVAAAAIYMASQASAEKKTQKEIGDIAGVADVTIRQSYRLIYPRAADLFPPDF--KFDTPVDKLP 314
[Close]
BLAST of Transcription initiation factor IIB vs. Ensembl Xenopus
Match: rars2 (arginyl-tRNA synthetase 2, mitochondrial [Source:Xenbase;Acc:XB-GENE-976890])

HSP 1 Score: 206.068 bits (523), Expect = 4.200e-63
Identity = 121/306 (39.54%), Postives = 171/306 (55.88%), Query Frame = 3 
Query:  108 QIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            ++ C  HP+A L+ED+ AGD+IC  CGLVVGDRVID  SEWR+F+ND   +D SRVG  +N   S    +  I         DE GN KY+N   MS  +R +      I    +R+N   N  +    L K++ E      ++  AI+ ACL++A R   VPRTFKE+CAVS +SKK+IG+  K ++ A+     +   G ++SRFCS L    K V   A+ IA  A    ++  +SP ++ A +IYMAS    E+R++K IGDI GV+  TI  +Y++ Y     L P     KF   V++LP
Sbjct:   12 KVTCPNHPDALLVEDYRAGDMICSECGLVVGDRVIDVGSEWRTFSNDKAAKDPSRVGDAQNPLLSGGDLTTMIGKGTGAASFDEFGNNKYQNRRTMSSSDRAMMNAFKEITNMADRINLPRNIVDRTNNLFKQVYEQKSLKGRSNDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETNVDLITTGDFMSRFCSNLGL-TKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPADF--KFDTPVDKLP 314
[Close]
BLAST of Transcription initiation factor IIB vs. Ensembl Xenopus
Match: rars2 (arginyl-tRNA synthetase 2, mitochondrial [Source:Xenbase;Acc:XB-GENE-976890])

HSP 1 Score: 205.682 bits (522), Expect = 6.520e-63
Identity = 121/306 (39.54%), Postives = 171/306 (55.88%), Query Frame = 3 
Query:  108 QIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            ++ C  HP+A L+ED+ AGD+IC  CGLVVGDRVID  SEWR+F+ND   +D SRVG  +N   S    +  I         DE GN KY+N   MS  +R +      I    +R+N   N  +    L K++ E      ++  AI+ ACL++A R   VPRTFKE+CAVS +SKK+IG+  K ++ A+     +   G ++SRFCS L    K V   A+ IA  A    ++  +SP ++ A +IYMAS    E+R++K IGDI GV+  TI  +Y++ Y     L P     KF   V++LP
Sbjct:   18 KVTCPNHPDALLVEDYRAGDMICSECGLVVGDRVIDVGSEWRTFSNDKAAKDPSRVGDAQNPLLSGGDLTTMIGKGTGAASFDEFGNNKYQNRRTMSSSDRAMMNAFKEITNMADRINLPRNIVDRTNNLFKQVYEQKSLKGRSNDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETNVDLITTGDFMSRFCSNLGL-TKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPADF--KFDTPVDKLP 320
[Close]
BLAST of Transcription initiation factor IIB vs. Ensembl Mouse
Match: Gtf2b (general transcription factor IIB [Source:MGI Symbol;Acc:MGI:2385191])

HSP 1 Score: 209.534 bits (532), Expect = 1.632e-64
Identity = 123/306 (40.20%), Postives = 172/306 (56.21%), Query Frame = 3 
Query:  108 QIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            ++ C  HP+A L+ED+ AGD+ICP CGLVVGDRVID  SEWR+F+ND   +D SRVG  +N   S+   S  I         DE GN KY+N   MS  +R +      I    +R+N   N  +    L K++ E      +   AI+ ACL++A R   VPRTFKE+CAVS +SKK+IG+  K ++ A+     +   G ++SRFCS L    K V   A+ IA  A    ++  +SP ++ A +IYMAS    E+R++K IGDI GV+  TI  +Y++ Y     L P     KF   V++LP
Sbjct:   12 RVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLP-KQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPSDF--KFDTPVDKLP 314
[Close]
BLAST of Transcription initiation factor IIB vs. Ensembl Mouse
Match: Brf1 (BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit [Source:MGI Symbol;Acc:MGI:1919558])

HSP 1 Score: 49.6766 bits (117), Expect = 5.687e-6
Identity = 54/211 (25.59%), Postives = 86/211 (40.76%), Query Frame = 3 
Query:  150 DFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAA-ISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNF--KNKFVSTLA 773
            D   GD +C  CG V+ D +I   SE +   N G G  SS VG   + + +  T ++G                 +  ++      + L+  R HI+  G +L  + +  + A     K+A S    R  K A +  ACL+L  R    P    ++  +  V+   +GKT   +   + +     D  +Y+ RF   L F  KN  VS  A
Sbjct:   17 DAARGDAVCTGCGSVLEDNII--VSEVQFVENSGGG--SSAVGQFVSLDGAGKTPTLG---------------GGFHVNLGKESRAQTLQNGRRHIHHLGSQLQLNQHCLDTAFNFF-KMAVSKHLTRGRKMAHVIAACLYLVCRTEGTPHMLLDLSDLLQVNVYVLGKTFLLLARELCINAPAIDPCLYIPRFAHLLEFGEKNHEVSMTA 207
[Close]
BLAST of Transcription initiation factor IIB vs. UniProt/SwissProt
Match: sp|Q5R886|TF2B_PONAB (Transcription initiation factor IIB OS=Pongo abelii OX=9601 GN=GTF2B PE=2 SV=1)

HSP 1 Score: 209.92 bits (533), Expect = 6.969e-64
Identity = 123/306 (40.20%), Postives = 172/306 (56.21%), Query Frame = 3 
Query:  108 QIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            ++ C  HP+A L+ED+ AGD+ICP CGLVVGDRVID  SEWR+F+ND   +D SRVG  +N   S+   S  I         DE GN KY+N   MS  +R +      I    +R+N   N  +    L K++ E      +   AI+ ACL++A R   VPRTFKE+CAVS +SKK+IG+  K ++ A+     +   G ++SRFCS L    K V   A+ IA  A    ++  +SP ++ A +IYMAS    E+R++K IGDI GV+  TI  +Y++ Y     L P     KF   V++LP
Sbjct:   12 RVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLP-KQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDF--KFDTPVDKLP 314
[Close]
BLAST of Transcription initiation factor IIB vs. UniProt/SwissProt
Match: sp|Q4R3J5|TF2B_MACFA (Transcription initiation factor IIB OS=Macaca fascicularis OX=9541 GN=GTF2B PE=2 SV=1)

HSP 1 Score: 209.92 bits (533), Expect = 6.969e-64
Identity = 123/306 (40.20%), Postives = 172/306 (56.21%), Query Frame = 3 
Query:  108 QIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            ++ C  HP+A L+ED+ AGD+ICP CGLVVGDRVID  SEWR+F+ND   +D SRVG  +N   S+   S  I         DE GN KY+N   MS  +R +      I    +R+N   N  +    L K++ E      +   AI+ ACL++A R   VPRTFKE+CAVS +SKK+IG+  K ++ A+     +   G ++SRFCS L    K V   A+ IA  A    ++  +SP ++ A +IYMAS    E+R++K IGDI GV+  TI  +Y++ Y     L P     KF   V++LP
Sbjct:   12 RVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLP-KQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDF--KFDTPVDKLP 314
[Close]
BLAST of Transcription initiation factor IIB vs. UniProt/SwissProt
Match: sp|Q00403|TF2B_HUMAN (Transcription initiation factor IIB OS=Homo sapiens OX=9606 GN=GTF2B PE=1 SV=1)

HSP 1 Score: 209.92 bits (533), Expect = 6.969e-64
Identity = 123/306 (40.20%), Postives = 172/306 (56.21%), Query Frame = 3 
Query:  108 QIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            ++ C  HP+A L+ED+ AGD+ICP CGLVVGDRVID  SEWR+F+ND   +D SRVG  +N   S+   S  I         DE GN KY+N   MS  +R +      I    +R+N   N  +    L K++ E      +   AI+ ACL++A R   VPRTFKE+CAVS +SKK+IG+  K ++ A+     +   G ++SRFCS L    K V   A+ IA  A    ++  +SP ++ A +IYMAS    E+R++K IGDI GV+  TI  +Y++ Y     L P     KF   V++LP
Sbjct:   12 RVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLP-KQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDF--KFDTPVDKLP 314
[Close]
BLAST of Transcription initiation factor IIB vs. UniProt/SwissProt
Match: sp|Q2KIN8|TF2B_BOVIN (Transcription initiation factor IIB OS=Bos taurus OX=9913 GN=GTF2B PE=2 SV=1)

HSP 1 Score: 209.92 bits (533), Expect = 8.098e-64
Identity = 122/306 (39.87%), Postives = 171/306 (55.88%), Query Frame = 3 
Query:  108 QIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            ++ C  HP+A L+ED+ AGD+ICP CGLVVGDRVID  SEWR+F+ND   +D SRVG  +N   S+   S  I         DE GN KY+N   MS  +R +      I    +R+N   N  +    L K++ E      +   AI+ ACL++A R   VPRTFKE+CAVS +SKK+IG+  K ++ A+     +   G ++SRFCS L    K V   A+ IA  A    ++  +SP ++   +IYMAS    E+R++K IGDI GV+  TI  +Y++ Y     L P     KF   V++LP
Sbjct:   12 RVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLP-KQVQMAATHIARKAVELDLVPGRSPISVAEAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDF--KFDTPVDKLP 314
[Close]
BLAST of Transcription initiation factor IIB vs. UniProt/SwissProt
Match: sp|P62916|TF2B_RAT (Transcription initiation factor IIB OS=Rattus norvegicus OX=10116 GN=Gtf2b PE=2 SV=1)

HSP 1 Score: 209.534 bits (532), Expect = 1.142e-63
Identity = 123/306 (40.20%), Postives = 172/306 (56.21%), Query Frame = 3 
Query:  108 QIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            ++ C  HP+A L+ED+ AGD+ICP CGLVVGDRVID  SEWR+F+ND   +D SRVG  +N   S+   S  I         DE GN KY+N   MS  +R +      I    +R+N   N  +    L K++ E      +   AI+ ACL++A R   VPRTFKE+CAVS +SKK+IG+  K ++ A+     +   G ++SRFCS L    K V   A+ IA  A    ++  +SP ++ A +IYMAS    E+R++K IGDI GV+  TI  +Y++ Y     L P     KF   V++LP
Sbjct:   12 RVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLP-KQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPSDF--KFDTPVDKLP 314
[Close]
BLAST of Transcription initiation factor IIB vs. TrEMBL
Match: G3VSF2 (General transcription factor IIB OS=Sarcophilus harrisii OX=9305 GN=GTF2B PE=4 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 4.288e-63
Identity = 125/315 (39.68%), Postives = 178/315 (56.51%), Query Frame = 3 
Query:   81 FLLNIMKSNQIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            F L+++   ++ C  HP+A L+ED+ AGD+ICP CGLVVGDRVID  SEWR+F+ND   +D SRVG  +N   S+   +  I         DE GN KY+NH  MS  +R +      I    +R+N   N  +    L K++ E      +   AI+ ACL++A R   VPRTFKE+CAVS +SKK+IG+  K ++ A+     +   G ++SRFCS L    K V   A+ IA  A    ++  +SP ++ A +IYMAS    E+R++K IGDI GV+  TI  +Y++ Y     L P     KF   V++LP
Sbjct:    5 FRLDVLP--RVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLTTMIGKGTGAASFDEFGNSKYQNHRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLGLP-KQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPADF--KFDTPVDKLP 314
[Close]
BLAST of Transcription initiation factor IIB vs. TrEMBL
Match: A0A5F9C904 (Kynurenine aminotransferase 3 OS=Oryctolagus cuniculus OX=9986 GN=KYAT3 PE=4 SV=1)

HSP 1 Score: 211.46 bits (537), Expect = 4.958e-62
Identity = 124/306 (40.52%), Postives = 173/306 (56.54%), Query Frame = 3 
Query:  108 QIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            ++ C  HP+A L+ED+ AGD+ICP CGLVVGDRVID  SEWR+F+ND   +D SRVG  +N   S+   S  I         DE GN KY+N  AMS  +R +      I    +R+N   N  +    L K++ E      +   AI+ ACL++A R   VPRTFKE+CAVS +SKK+IG+  K ++ A+     +   G ++SRFCS L    K V   A+ IA  A    ++  +SP ++ A +IYMAS    E+R++K IGDI GV+  TI  +Y++ Y     L P     KF   V++LP
Sbjct:   12 RVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRAMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLP-KQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDF--KFDTPVDKLP 314
[Close]
BLAST of Transcription initiation factor IIB vs. TrEMBL
Match: J3SDI3 (Transcription initiation factor IIB-like OS=Crotalus adamanteus OX=8729 PE=2 SV=1)

HSP 1 Score: 211.075 bits (536), Expect = 7.865e-62
Identity = 120/306 (39.22%), Postives = 173/306 (56.54%), Query Frame = 3 
Query:  108 QIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            ++ C  HP++ L+ED+ AGD+ICP CGLVVGDRVID  SEWR+F+ND   +D SRVG  +N   S+   +  I         DE GN KY+N   MS  +R +      I    +R+N   N  +    L K++ E      ++  AI+ ACL++A R   VPRTFKE+CAVS +SKK+IG+  K ++ A+     +   G ++SRFCS L    K V   A+ IA  A    ++  +SP ++ A +IYMAS    E+R++K IGDI GV+  TI  +Y++ Y     L P     +F   V++LP
Sbjct:   12 RVTCPNHPDSTLVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDTQNPLLSDGDLTTMIGKGTGAASFDEFGNSKYQNRRTMSSFDRAMMNAFKEIASMADRINLPRNIVDRTNNLFKQVYEQKSLKGRSNDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLGLP-KQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPADF--RFDTPVDKLP 314
[Close]
BLAST of Transcription initiation factor IIB vs. TrEMBL
Match: A0A1S3AKP9 (transcription initiation factor IIB OS=Erinaceus europaeus OX=9365 GN=GTF2B PE=4 SV=1)

HSP 1 Score: 210.69 bits (535), Expect = 9.958e-62
Identity = 123/306 (40.20%), Postives = 172/306 (56.21%), Query Frame = 3 
Query:  108 QIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            ++ C  HP+A L+ED+ AGD+ICP CGLVVGDRVID  SEWR+F+ND   +D SRVG  +N   S+   S  I         DE GN KY+N   MS  +R +      I    +R+N   N  +    L K++ E      +   AI+ ACL++A R   VPRTFKE+CAVS +SKK+IG+  K ++ A+     +   G ++SRFCS L    K V   A+ IA  A    ++  +SP ++ A +IYMAS    E+R++K IGDI GV+  TI  +Y++ Y     L P     KF   V++LP
Sbjct:   12 RVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDAQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLP-KQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDF--KFDTPVDKLP 314
[Close]
BLAST of Transcription initiation factor IIB vs. TrEMBL
Match: E9IQ81 (TFIIB-type domain-containing protein (Fragment) OS=Solenopsis invicta OX=13686 GN=SINV_13223 PE=4 SV=1)

HSP 1 Score: 210.69 bits (535), Expect = 1.139e-61
Identity = 128/310 (41.29%), Postives = 178/310 (57.42%), Query Frame = 3 
Query:  102 SNQIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENA--NNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            +N+I C  HP+A LIED+ AGD IC  CGLVVGDRVID  SEWR+F+N+ +G D SRVG  EN   N ++ +T IG          D  G  KY+N   MS  +R L      IN   +R+N      + A  L K++ +      +   AI+ ACL++A R   VPRTFKE+CAVS +SKK+IG+  K ++ A+     +   G ++SRFCS L   N  V   A+ IA  A    I+  +SP ++ A +IYMAS    ++RS+K IGDI GV+  TI  +YK+ Y + + L P     KFA  +++LP
Sbjct:    4 TNKICCYAHPDATLIEDYRAGDQICSECGLVVGDRVIDVGSEWRTFSNEKSGVDPSRVGGPENPLLNGADLSTMIGPGTG--AASFDGFGTAKYQNRRTMSSSDRALLNAFREINGMADRINLPKTIVDRANALFKQVHDGKNLKGRANDAIASACLYIACRQEGVPRTFKEICAVSKISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLGLPN-MVQKAATHIARKAVEIDIVPGRSPISVAAAAIYMASQASEDKRSQKEIGDIAGVADVTIRQSYKLMYPHANKLFPEDF--KFATPIDQLP 308
[Close]
BLAST of Transcription initiation factor IIB vs. Ensembl Cavefish
Match: gtf2b (general transcription factor IIB [Source:NCBI gene;Acc:103039385])

HSP 1 Score: 141.739 bits (356), Expect = 8.694e-40
Identity = 74/189 (39.15%), Postives = 106/189 (56.08%), Query Frame = 3 
Query:  108 QIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENA--NNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKV 668
            ++ C  HP A L+ED+ AGD+ICP CGLVVGDRVID  SEWR+F N+   +D SRVG  +N   N  + TT I           DE GN KY+N   MS  +R +      I+   +R+N   N  +    L K++ E      ++  AI+ ACL++A R   VPRTFKE+  ++GV+   I ++ + +
Sbjct:   12 RVQCPNHPEAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFTNEKATKDPSRVGDAQNPLLNGGDLTTMIS--KGTGAASFDEFGNSKYQNRRTMSSSDRAMLNAFKEISTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRSNDAIASACLYIACRQEGVPRTFKEIGDIAGVADVTIRQSYRLI 198
[Close]
BLAST of Transcription initiation factor IIB vs. Ensembl Cavefish
Match: brf1a (transcription factor IIIB 90 kDa subunit-like [Source:NCBI gene;Acc:103040939])

HSP 1 Score: 50.0618 bits (118), Expect = 2.959e-6
Identity = 56/216 (25.93%), Postives = 84/216 (38.89%), Query Frame = 3 
Query:  117 CKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMER--MLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAA-ISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNK 755
            CK     N+  D T GD +C  CG V+ D +I   SE     N G G  SS VG    ++ S    S+G                   N   M    R   L+  + +IN  G +L  + +  N A    K +A      R  K+A +  ACL+L  R    P    ++  +  V+   +G+T   +   + +     D  +Y+ RF   L F  K
Sbjct:    4 CKNCGGTNIDVDQTRGDAVCMGCGSVLEDNII--VSEVTFVENSGGG--SSAVGQFVASDASGNAPSLG------------------GNFTGMGKESRAQTLQNAKRNINHLGHQLQMNQHCLNTAFNFYK-MALCKRLTRGRKSAHVIAACLYLVCRTEGTPHMLLDLSDLLQVNVYVLGRTFLALAKELCINAPAIDPCLYVPRFAQMLEFGEK 196
[Close]
BLAST of Transcription initiation factor IIB vs. Ensembl Yeast
Match: SUA7 (Transcription factor TFIIB; a general transcription factor required for transcription initiation and start site selection by RNA polymerase II [Source:SGD;Acc:S000006290])

HSP 1 Score: 110.538 bits (275), Expect = 7.161e-28
Identity = 96/319 (30.09%), Postives = 153/319 (47.96%), Query Frame = 3 
Query:  114 HCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDG-TGEDSSRVGAVENA--NNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTV---KKVISAIGVEGTVR-DVG--------VYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
             CK +P   ++E F+ GD++C +CGLV+ D+++D  SEWR+F+ND   G+D SRVG   N   + +  +T IG   + D+R   E    + KN   M   +  ++     I    +        ++ A    K   +      K+  +I  A + +  R   V RTFKE+ ++  V  K+ GKT+   K ++     +G ++ D           Y+ RFCS L    + V+T A   A      K I+ KSP TI   SIY+  LL     +   +G  L V++GTI + YKI Y +R  LV  ++ +    S++ LP
Sbjct:   26 ECKVYP-PKIVERFSEGDVVCALCGLVLSDKLVDTRSEWRTFSNDDHNGDDPSRVGEASNPLLDGNNLSTRIGKGETTDMRFTKELNKAQGKN--VMDKKDNEVQAAFAKITMLCDAAELPKIVKDCAKEAYKLCHDEKTLKGKSMESIMAASILIGCRRAEVARTFKEIQSLIHVKTKEFGKTLNIMKNILRGKSEDGFLKIDTDNMSGAQNLTYIPRFCSHLGLPMQ-VTTSAEYTAKKCKEIKEIAGKSPITIAVVSIYLNILLFQIPITAAKVGQTLQVTEGTIKSGYKILYEHRDKLVDPQLIANGVVSLDNLP 340
[Close]
BLAST of Transcription initiation factor IIB vs. Ensembl Nematostella
Match: EDO36932 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SH79])

HSP 1 Score: 164.466 bits (415), Expect = 7.482e-48
Identity = 113/313 (36.10%), Postives = 169/313 (53.99%), Query Frame = 3 
Query:   93 IMKSNQIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELPSA 1031
            + +   + C++HP+A LIED+ AGD+IC  CGLVVGDRV+D  SEWR+F+N+    D SRVGA +N   S    S  I+         E   + + +   MS  +R L      I+   +R+N      + A  L K++ E      ++   I+ ACL++A R   VPRT KE+CA+  +SKK+IG+  K ++  +     +   G ++SRFCS L      V   A+ IA  A    +++ +SP +I A +IYMAS    E+RS+K IGDI GV+  TI  +Y++ +     L P     KFA  +  LPS+
Sbjct:    1 MTREKSVACRHHPDAPLIEDYRAGDMICSECGLVVGDRVVDVGSEWRTFSNEKATSDPSRVGAAQNPLLSGGDLSTLIEKPSGGNADIEGFARTHNSRSGMSGADRSLMNAFREISIMADRINLPRKIVDRANQLFKQVHEQKSLKGRSNDTIASACLYIACRQEGVPRTLKEICAIGKISKKEIGRCFKLILKHLETSVDLITSGDFMSRFCSNLTLPLS-VQKAATYIARRAVELDLVAGRSPISIAAAAIYMASQASEEKRSQKEIGDIAGVADVTIRQSYRLLFPKARELFPADF--KFAVPIERLPSS 310
[Close]
BLAST of Transcription initiation factor IIB vs. Ensembl Medaka
Match: gtf2b (general transcription factor IIB [Source:NCBI gene;Acc:101158724])

HSP 1 Score: 188.734 bits (478), Expect = 1.287e-56
Identity = 120/309 (38.83%), Postives = 169/309 (54.69%), Query Frame = 3 
Query:  108 QIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENA--NNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGD-ILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELP 1025
            ++ C  HP+A L+ED+ AGD+ICP CGLVVGDRVID  SEWR+F+N+   +D SRVG  +N   N  + TT IG          DE GN KY+N   MS  +R +      I+   +R+N   N  +    L K++ E      +   AI+ ACL++A R   VPRTFKE+CAVS +SKK+IG+  K ++ A+     +   G ++SRFCS L    K V   A+ IA  A    ++  +SP ++ A +IYMA        S+   GD I GV+  TI  +Y++ Y     L P     KF   V++LP
Sbjct:   14 RVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNEKAQKDPSRVGDAQNPLLNGGDLTTMIG--KGTGSASFDEFGNSKYQNRRTMSSSDRAMLHAFKEISTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLGLP-KQVQMAATFIARKAVELDLVPGRSPISVAAAAIYMA--------SQASAGDHIAGVADVTIRQSYRLIYPRAAELFPPDF--KFDTPVDKLP 309
[Close]
BLAST of Transcription initiation factor IIB vs. Planmine SMEST
Match: SMESG000077013.1 (SMESG000077013.1)

HSP 1 Score: 611.683 bits (1576), Expect = 0.000e+0
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 3 
Query:   96 MKSNQIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELPSA 1031
            MKSNQIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKIISSKSPTTIIATSIYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELPSA
Sbjct:    1 MKSNQIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKIISSKSPTTIIATSIYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELPSA 312
[Close]
BLAST of Transcription initiation factor IIB vs. Planmine SMEST
Match: SMESG000077016.1 (SMESG000077016.1)

HSP 1 Score: 249.595 bits (636), Expect = 1.626e-80
Identity = 142/314 (45.22%), Postives = 198/314 (63.06%), Query Frame = 3 
Query:   96 MKSNQIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKK---VISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVPLKMASKFAKSVNELPS 1028
            M SN++HCKYHP A LIED TAGDL+C  CGLVV +RVID  SEWRSFAND   +D SRVG VE    SE  TSI I+ S ++RHLDE+GNQ YK+   +S  +R LK   ++I   G+R+N   N  + AMGL K++ E+ +   +N  A++ +CLF+A R   VPRTFKE+CAVS VSKKDIGK  KK   ++S      T  D   +++RFC +L      +  +A EI+  A ++ +++ +SP +I A SIYMASL M  ++ ++ IG++ GV++GT+   YK+   +   L P          +N+LPS
Sbjct:    1 MSSNKVHCKYHPTATLIEDHTAGDLVCTRCGLVVAERVIDFESEWRSFANDD-AQDMSRVGEVETGLKSETETSITIQQSSNIRHLDESGNQIYKSRSTLSSSDRALKTAHSNIRAMGDRINLPKNLTDLAMGLFKQVHETKQLRGRNNEAVATSCLFMACRQLKVPRTFKEICAVSNVSKKDIGKIFKKIGRILSTSVGNATGED---FMARFCYQLGIPR--LERIAKEISKKALDDNMVAGRSPVSIAAASIYMASLAMGIKKDKRDIGEVAGVAEGTLNVVYKLMRPHVEKLFPPNFTP--VVPLNQLPS 306
[Close]
BLAST of Transcription initiation factor IIB vs. Planmine SMEST
Match: SMESG000019348.1 (SMESG000019348.1)

HSP 1 Score: 150.599 bits (379), Expect = 2.379e-42
Identity = 97/295 (32.88%), Postives = 157/295 (53.22%), Query Frame = 3 
Query:  105 NQIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGT-GEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVG--VYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVP 980
            N +HCKYHP   LI D   GD++C  CGL+V DR ID SSEWR+FA+DG  G D+ RVGAVE    ++  TS  ++   ++R LDENGN K+K   + +  E  L   + +I +  ER+  D N  + ++ L+ ++ + N  +++NKA ++C CL+LA  DN VPR  KE+     +  +   K + K++  I ++   +++   + ++RF   L+  +  +  L  E     S    + SK    I    I +   L   +    ++ D LG+S+  IA   KI + N   ++P
Sbjct:    2 NIVHCKYHPYKVLITDIRTGDIVCSACGLIVVDRAIDPSSEWRTFASDGADGHDACRVGAVE-TEETDGQTSTTVQTGFNLRFLDENGNSKFKYLSSSTNKEVSLASQKRNIADLCERIKVDRNVYSRSVFLLHEIIKLNAVSKRNKATLNCVCLYLACLDNGVPRLLKEIINECPLVDEKKFKKLLKLVQ-ITLKRPTQEINPILLIARFLYYLDVTDFKIENLVKEAVKNVSKISELRSKGNVMIAVYCIQLVCDLKRVDVDASLLADSLGLSKSNIAMFSKILHKNVKTILP 294
[Close]
BLAST of Transcription initiation factor IIB vs. Planmine SMEST
Match: SMESG000019400.1 (SMESG000019400.1)

HSP 1 Score: 147.517 bits (371), Expect = 3.044e-41
Identity = 94/293 (32.08%), Postives = 155/293 (52.90%), Query Frame = 3 
Query:  111 IHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGT-GEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAMGLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKKDIGKTVKKVISAIGVEGTVRDVG--VYLSRFCSRLNFKNKFVSTLASEIATLASNNKXXXXXXXXXXXXXXXYMASLLMNEERSRKIIGDILGVSQGTIATTYKIFYLNRHVLVP 980
            +HCKYHP   LI D   GD++C  CGL+V DR ID SSEWR+FA+DG  G D+ RVGAVE    ++  TS  ++   ++R LDENGN K+K   + +  E  L   + +I +  ER+  D N  + ++ L+ ++ + N   ++NK A++C CL+LA  +N VPR  KE+     +  +   K + K++  I ++   +++   + ++RF   L+  +  +  L  E     S    +  K    I    I +   L   +   K++ D LG+S+  I    KI + N  +++P
Sbjct:    2 VHCKYHPYKVLITDIRTGDIVCSACGLIVVDRAIDPSSEWRTFASDGADGHDACRVGAVE-TEETDGQTSTTVQTGFNLRFLDENGNSKFKYLSSSTNKEVSLASQKRNIADLCERIKVDRNVYSRSVLLLHEITKLNVVGKRNKVALNCVCLYLACLENCVPRLLKEILNECPLVDEKKFKKLLKLVQ-ITLKRPTQEINPILLIARFLYYLDVTDFKIENLVKEAVKNVSKISELRCKGNVMIAVCCIQLVCDLKRVDVDAKLLADSLGLSKSNIDMFSKILHKNVKIILP 292
[Close]
BLAST of Transcription initiation factor IIB vs. Planmine SMEST
Match: SMESG000077015.1 (SMESG000077015.1)

HSP 1 Score: 117.472 bits (293), Expect = 5.240e-32
Identity = 54/89 (60.67%), Postives = 67/89 (75.28%), Query Frame = 3 
Query:   96 MKSNQIHCKYHPNANLIEDFTAGDLICPVCGLVVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHDVRHLDEN 362
            M SN++HCKYHP A LIED TAGDL+C +CGLVV +RVID+ SEWRSFAND   E   RVG +E+A  SE  TSI ++ S ++R LDE+
Sbjct:    1 MSSNKVHCKYHPTATLIEDHTAGDLVCTLCGLVVAERVIDYESEWRSFANDDNQE-MCRVGEMESAFRSETETSIMMQQSSNIRLLDES 88
The following BLAST results are available for this feature:
BLAST of Transcription initiation factor IIB vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
GTF2B1.451e-6440.20general transcription factor IIB [Source:HGNC Symb... [more]
GTF2B5.021e-4941.26general transcription factor IIB [Source:HGNC Symb... [more]
GTF2B9.075e-4739.38general transcription factor IIB [Source:HGNC Symb... [more]
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BLAST of Transcription initiation factor IIB vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ttb-13.659e-5937.50Transcription initiation factor IIB [Source:UniPr... [more]
ttb-13.659e-5937.50Transcription initiation factor IIB [Source:UniPr... [more]
ttb-13.659e-5937.50Transcription initiation factor IIB [Source:UniPr... [more]
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BLAST of Transcription initiation factor IIB vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
TfIIB2.012e-5938.39gene:FBgn0004915 transcript:FBtr0080025[more]
TfIIB2.012e-5938.39gene:FBgn0004915 transcript:FBtr0333489[more]
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BLAST of Transcription initiation factor IIB vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gtf2b4.429e-6339.61general transcription factor IIB [Source:ZFIN;Acc:... [more]
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BLAST of Transcription initiation factor IIB vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rars24.200e-6339.54arginyl-tRNA synthetase 2, mitochondrial [Source:X... [more]
rars26.520e-6339.54arginyl-tRNA synthetase 2, mitochondrial [Source:X... [more]
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BLAST of Transcription initiation factor IIB vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Gtf2b1.632e-6440.20general transcription factor IIB [Source:MGI Symbo... [more]
Brf15.687e-625.59BRF1, RNA polymerase III transcription initiation ... [more]
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BLAST of Transcription initiation factor IIB vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q5R886|TF2B_PONAB6.969e-6440.20Transcription initiation factor IIB OS=Pongo abeli... [more]
sp|Q4R3J5|TF2B_MACFA6.969e-6440.20Transcription initiation factor IIB OS=Macaca fasc... [more]
sp|Q00403|TF2B_HUMAN6.969e-6440.20Transcription initiation factor IIB OS=Homo sapien... [more]
sp|Q2KIN8|TF2B_BOVIN8.098e-6439.87Transcription initiation factor IIB OS=Bos taurus ... [more]
sp|P62916|TF2B_RAT1.142e-6340.20Transcription initiation factor IIB OS=Rattus norv... [more]
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BLAST of Transcription initiation factor IIB vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
G3VSF24.288e-6339.68General transcription factor IIB OS=Sarcophilus ha... [more]
A0A5F9C9044.958e-6240.52Kynurenine aminotransferase 3 OS=Oryctolagus cunic... [more]
J3SDI37.865e-6239.22Transcription initiation factor IIB-like OS=Crotal... [more]
A0A1S3AKP99.958e-6240.20transcription initiation factor IIB OS=Erinaceus e... [more]
E9IQ811.139e-6141.29TFIIB-type domain-containing protein (Fragment) OS... [more]
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BLAST of Transcription initiation factor IIB vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
gtf2b8.694e-4039.15general transcription factor IIB [Source:NCBI gene... [more]
brf1a2.959e-625.93transcription factor IIIB 90 kDa subunit-like [Sou... [more]
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BLAST of Transcription initiation factor IIB vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transcription initiation factor IIB vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
SUA77.161e-2830.09Transcription factor TFIIB; a general transcriptio... [more]
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BLAST of Transcription initiation factor IIB vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO369327.482e-4836.10Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Transcription initiation factor IIB vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gtf2b1.287e-5638.83general transcription factor IIB [Source:NCBI gene... [more]
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BLAST of Transcription initiation factor IIB vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000077013.10.000e+0100.00SMESG000077013.1[more]
SMESG000077016.11.626e-8045.22SMESG000077016.1[more]
SMESG000019348.12.379e-4232.88SMESG000019348.1[more]
SMESG000019400.13.044e-4132.08SMESG000019400.1[more]
SMESG000077015.15.240e-3260.67SMESG000077015.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012239 ID=SMED30012239|Name=Transcription initiation factor IIB|organism=Schmidtea mediterranea sexual|type=transcript|length=1163bp
AAAGTCTAAAAATATGATTTCTAAAATCTTCGGAAATTGCGATGCGATTG
TTTAACTATGTGAAAATTTCAATCAATGGATTTTTGTTGAATATTATGAA
ATCGAACCAAATTCATTGCAAATATCATCCAAATGCAAATTTAATTGAGG
ATTTCACTGCCGGCGATTTGATTTGTCCCGTATGTGGATTAGTTGTCGGT
GATAGAGTCATTGATCACAGCAGTGAATGGCGTTCATTTGCCAATGATGG
AACAGGGGAAGATTCATCGAGAGTCGGTGCAGTGGAAAACGCTAACAATT
CAGAAGCTACAACGTCAATTGGCATCAAAAACTCGCATGATGTTCGGCAT
TTAGACGAAAATGGTAACCAGAAATATAAGAATCACGTTGCCATGTCACC
AATGGAAAGAATGCTGAAACAATGCCGTAACCATATCAACGAATATGGTG
AGAGATTGAATGCCGATTCTAATAATAGAAATCATGCAATGGGACTCGTC
AAAAAACTCGCGGAATCCAACGAATTTAACCGGAAAAACAAAGCAGCAAT
TTCTTGTGCTTGTTTATTTTTGGCATTTCGAGATAATTCAGTTCCGAGGA
CGTTTAAAGAAATGTGTGCTGTGTCAGGAGTTTCTAAAAAAGATATTGGA
AAAACGGTTAAGAAAGTTATTTCGGCAATTGGAGTCGAAGGAACCGTAAG
AGATGTTGGAGTGTATCTTTCTCGATTCTGTAGCAGACTCAATTTTAAAA
ACAAATTTGTGTCAACTTTAGCTAGTGAAATTGCAACACTCGCATCTAAC
AATAAAATCATATCTAGCAAGAGTCCTACAACAATTATTGCTACCTCTAT
TTATATGGCTAGTCTTTTAATGAACGAAGAGCGTAGTAGAAAGATTATAG
GAGATATTCTCGGTGTTTCTCAAGGAACAATCGCAACAACCTATAAAATA
TTCTATTTGAATAGGCATGTATTGGTTCCTTTAAAAATGGCTTCCAAATT
CGCAAAGTCTGTCAATGAACTTCCTTCAGCTTGAAAGTTTTGACAGTGTT
CTGCTAATAGATTGTTTTGTAAATGTATTTCATTTTAATTAATAATCTGG
GATAATAAGCTCGACCGAATATTAAAAGAATTTCTGCCATGAATTATTAA
ATAAGGACGAGCG
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protein sequence of SMED30012239-orf-1

>SMED30012239-orf-1 ID=SMED30012239-orf-1|Name=SMED30012239-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=331bp
MRLFNYVKISINGFLLNIMKSNQIHCKYHPNANLIEDFTAGDLICPVCGL
VVGDRVIDHSSEWRSFANDGTGEDSSRVGAVENANNSEATTSIGIKNSHD
VRHLDENGNQKYKNHVAMSPMERMLKQCRNHINEYGERLNADSNNRNHAM
GLVKKLAESNEFNRKNKAAISCACLFLAFRDNSVPRTFKEMCAVSGVSKK
DIGKTVKKVISAIGVEGTVRDVGVYLSRFCSRLNFKNKFVSTLASEIATL
ASNNKIISSKSPTTIIATSIYMASLLMNEERSRKIIGDILGVSQGTIATT
YKIFYLNRHVLVPLKMASKFAKSVNELPSA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0070897DNA-templated transcriptional preinitiation complex assembly
GO:0006355regulation of transcription, DNA-templated
GO:0006352DNA-templated transcription, initiation
GO:0006413translational initiation
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR036915Cyclin-like_sf
IPR000812TFIIB
IPR013763Cyclin-like
IPR013150TFIIB_cyclin
IPR013137Znf_TFIIB
Vocabulary: molecular function
TermDefinition
GO:0008270zinc ion binding
GO:0017025TBP-class protein binding
GO:0003743translation initiation factor activity
GO:0046872metal ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000812Transcription factor TFIIBPRINTSPR00685TIFACTORIIBcoord: 56..77
score: 33.33
coord: 154..173
score: 47.33
coord: 242..258
score: 49.02
coord: 42..55
score: 40.95
coord: 207..225
score: 29.12
coord: 274..288
score: 36.44
coord: 20..40
score: 52.06
coord: 180..195
score: 30.42
IPR000812Transcription factor TFIIBPANTHERPTHR11618TRANSCRIPTION INITIATION FACTOR IIB-RELATEDcoord: 5..297
IPR013763Cyclin-likeSMARTSM00385cyclin_7coord: 206..288
e-value: 4.4E-4
score: 29.6
coord: 112..193
e-value: 4.4E-5
score: 32.9
IPR013763Cyclin-likeCDDcd00043CYCLINcoord: 106..191
e-value: 1.09174E-8
score: 49.5445
IPR013763Cyclin-likeCDDcd00043CYCLINcoord: 200..287
e-value: 5.79554E-7
score: 44.9221
NoneNo IPR availableGENE3DG3DSA:1.10.472.10coord: 205..311
e-value: 9.0E-19
score: 69.5
NoneNo IPR availableGENE3DG3DSA:1.10.472.10coord: 106..198
e-value: 2.0E-22
score: 81.1
NoneNo IPR availableGENE3DG3DSA:2.20.25.10coord: 1..51
e-value: 1.8E-22
score: 80.6
NoneNo IPR availablePANTHERPTHR11618:SF13TRANSCRIPTION INITIATION FACTOR IIBcoord: 5..297
NoneNo IPR availableSUPERFAMILYSSF57783Zinc beta-ribboncoord: 4..51
IPR013137Zinc finger, TFIIB-typePFAMPF08271TF_Zn_Ribboncoord: 8..48
e-value: 1.3E-12
score: 47.0
IPR013137Zinc finger, TFIIB-typePROSITEPS51134ZF_TFIIBcoord: 3..35
score: 8.606
IPR013150Transcription factor TFIIB, cyclin-like domainPFAMPF00382TFIIBcoord: 128..184
e-value: 2.0E-7
score: 31.0
coord: 209..279
e-value: 1.0E-14
score: 54.3
IPR036915Cyclin-like superfamilySUPERFAMILYSSF47954Cyclin-likecoord: 110..195
IPR036915Cyclin-like superfamilySUPERFAMILYSSF47954Cyclin-likecoord: 203..300