NGFI-A-binding protein 1

Overview
NameNGFI-A-binding protein 1
Smed IDSMED30012203
Length (bp)1481
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of NGFI-A-binding protein 1 (SMED30012203) t-SNE clustered cells

Violin plots show distribution of expression levels for NGFI-A-binding protein 1 (SMED30012203) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of NGFI-A-binding protein 1 (SMED30012203) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for NGFI-A-binding protein 1 (SMED30012203) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30012203

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30012203h1SMcG0002697 dd_Smed_v4_3973_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30012203h1SMcG0002697 dd_Smed_v4_3973_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
muscle cellSMED30012203h1SMcG0002697 dd_Smed_v4_3973_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30012203h1SMcG0002697 dd_Smed_v4_3973_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30012203h1SMcG0002697 dd_Smed_v4_3973_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of NGFI-A-binding protein 1 vs. Ensembl Human
Match: NAB1 (NGFI-A binding protein 1 [Source:HGNC Symbol;Acc:HGNC:7626])

HSP 1 Score: 166.777 bits (421), Expect = 2.914e-45
Identity = 113/321 (35.20%), Postives = 165/321 (51.40%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNL-----VPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINT-------VSNLMNLPSFFNSSVF----SAPMNGRVKSLDICSGIPVSLLPNA-VLSNSEKSKIKLAAENLAQTLPKIDARINSTGR-----KDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
             E++LYR+L++ANL  YF  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+R+W  NP  FN+  T   +P+S+ P  K  +       + C   +R  N                    +P L +P       + +       V   + L S   S ++    +      +   D+  G P S   ++  L  +    +    E +A TLPK D  +N         K + K +  I  M + DP +E +I +YSAIYGRF++ R+ GK +  HE++VNEAAAQL ++   L+TRRD LF LARQ+ +
Sbjct:   10 GELQLYRILQKANLLSYFDAFIQQGGDDVQQLCEAGEEEFLEIMALVGMASKPLHVRRLQKALRDWVTNPGLFNQPLTS--LPVSSIPIYKLPEGSPTWLGISCSSYERSSN------------------AREPHLKIPKCAATTCVQSLGQGKSDVVGSLALQSVGESRLWQGHHATESEHSLSPADL--GSPASPKESSEALDAAAALSVAECVERMAPTLPKSD--LNEVKELLKTNKKLAKMIGHIFEMNDDDPHKEEEIRKYSAIYGRFDSKRKDGKHLTLHELTVNEAAAQLCVKDNALLTRRDELFALARQISR 306          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Human
Match: NAB1 (NGFI-A binding protein 1 [Source:HGNC Symbol;Acc:HGNC:7626])

HSP 1 Score: 166.777 bits (421), Expect = 2.914e-45
Identity = 113/321 (35.20%), Postives = 165/321 (51.40%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNL-----VPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINT-------VSNLMNLPSFFNSSVF----SAPMNGRVKSLDICSGIPVSLLPNA-VLSNSEKSKIKLAAENLAQTLPKIDARINSTGR-----KDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
             E++LYR+L++ANL  YF  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+R+W  NP  FN+  T   +P+S+ P  K  +       + C   +R  N                    +P L +P       + +       V   + L S   S ++    +      +   D+  G P S   ++  L  +    +    E +A TLPK D  +N         K + K +  I  M + DP +E +I +YSAIYGRF++ R+ GK +  HE++VNEAAAQL ++   L+TRRD LF LARQ+ +
Sbjct:   10 GELQLYRILQKANLLSYFDAFIQQGGDDVQQLCEAGEEEFLEIMALVGMASKPLHVRRLQKALRDWVTNPGLFNQPLTS--LPVSSIPIYKLPEGSPTWLGISCSSYERSSN------------------AREPHLKIPKCAATTCVQSLGQGKSDVVGSLALQSVGESRLWQGHHATESEHSLSPADL--GSPASPKESSEALDAAAALSVAECVERMAPTLPKSD--LNEVKELLKTNKKLAKMIGHIFEMNDDDPHKEEEIRKYSAIYGRFDSKRKDGKHLTLHELTVNEAAAQLCVKDNALLTRRDELFALARQISR 306          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Human
Match: NAB1 (NGFI-A binding protein 1 [Source:HGNC Symbol;Acc:HGNC:7626])

HSP 1 Score: 166.777 bits (421), Expect = 2.952e-45
Identity = 113/321 (35.20%), Postives = 165/321 (51.40%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNL-----VPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINT-------VSNLMNLPSFFNSSVF----SAPMNGRVKSLDICSGIPVSLLPNA-VLSNSEKSKIKLAAENLAQTLPKIDARINSTGR-----KDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
             E++LYR+L++ANL  YF  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+R+W  NP  FN+  T   +P+S+ P  K  +       + C   +R  N                    +P L +P       + +       V   + L S   S ++    +      +   D+  G P S   ++  L  +    +    E +A TLPK D  +N         K + K +  I  M + DP +E +I +YSAIYGRF++ R+ GK +  HE++VNEAAAQL ++   L+TRRD LF LARQ+ +
Sbjct:   10 GELQLYRILQKANLLSYFDAFIQQGGDDVQQLCEAGEEEFLEIMALVGMASKPLHVRRLQKALRDWVTNPGLFNQPLTS--LPVSSIPIYKLPEGSPTWLGISCSSYERSSN------------------AREPHLKIPKCAATTCVQSLGQGKSDVVGSLALQSVGESRLWQGHHATESEHSLSPADL--GSPASPKESSEALDAAAALSVAECVERMAPTLPKSD--LNEVKELLKTNKKLAKMIGHIFEMNDDDPHKEEEIRKYSAIYGRFDSKRKDGKHLTLHELTVNEAAAQLCVKDNALLTRRDELFALARQISR 306          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Human
Match: NAB2 (NGFI-A binding protein 2 [Source:HGNC Symbol;Acc:HGNC:7627])

HSP 1 Score: 152.14 bits (383), Expect = 3.772e-40
Identity = 113/333 (33.93%), Postives = 163/333 (48.95%), Query Frame = 2
Query:   29 MALTSNLCSNEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFD--SNIFMSQPLLPLPDDENNETINTVSNLMNLPSFF--------NSSVFSAPMNGRV--------KSLDICSGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLPKIDA-------RINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
            MAL   L   E++LYR+L+RANL  Y+  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+REW+ NP  F  S+    +P+S+ P  K ++            +     K      F   S + + + L PLP                  S           S  FS P  G V         +     G P  L P  V    E      + E + ++ P+ DA       ++N    K + + +  I  M ++D  +E +I +YS IYGRF++ RR GK ++ HE+++NEAAAQ  ++   L+ RR  LF L+RQV +
Sbjct:   34 MALPRTL--GELQLYRVLQRANLLSYYETFIQQGGDDVQQLCEAGEEEFLEIMALVGMATKPLHVRRLQKALREWATNPGLF--SQPVPAVPVSSIPLFKISETAGTRKGSMSNGHGSPGEKAGSARSFSPKSPLELGEKLSPLPGGPGAGDPRIWPGRSTPESDVGAGGEEEAGSPPFSPPAGGGVPEGTGAGGLAAGGTGGGPDRLEPEMVRMVVE------SVERIFRSFPRGDAGEVTSLLKLN----KKLARSVGHIFEMDDNDSQKEEEIRKYSIIYGRFDSKRREGKQLSLHELTINEAAAQFCMRDNTLLLRRVELFSLSRQVAR 352          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Human
Match: NAB2 (NGFI-A binding protein 2 [Source:HGNC Symbol;Acc:HGNC:7627])

HSP 1 Score: 151.369 bits (381), Expect = 1.737e-39
Identity = 113/329 (34.35%), Postives = 163/329 (49.54%), Query Frame = 2
Query:   29 MALTSNLCSNEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFD--SNIFMSQPLLPLPDDENNETINTVSNLMNLPSFF--------NSSVFSAPMNGRVK--------SLDICSGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLPKIDA-RINSTGR--KDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
            MAL   L   E++LYR+L+RANL  Y+  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+REW+ NP  F  S+    +P+S+ P  K ++            +     K      F   S + + + L PLP                  S           S  FS P  G V         +     G P  L P  V    E      + E + ++ P+ DA  + S  +  K + + +  I  M ++D  +E +I +YS IYGRF++ RR GK ++ HE+++NEAAAQ  ++   L+ RR  LF L+RQV +
Sbjct:   34 MALPRTL--GELQLYRVLQRANLLSYYETFIQQGGDDVQQLCEAGEEEFLEIMALVGMATKPLHVRRLQKALREWATNPGLF--SQPVPAVPVSSIPLFKISETAGTRKGSMSNGHGSPGEKAGSARSFSPKSPLELGEKLSPLPGGPGAGDPRIWPGRSTPESDVGAGGEEEAGSPPFSPPAGGGVPEGTGAGGLAAGGTGGGPDRLEPEMVRMVVE------SVERIFRSFPRGDAGEVTSLLKLNKKLARSVGHIFEMDDNDSQKEEEIRKYSIIYGRFDSKRREGKQLSLHELTINEAAAQFCMRDNTLLLRRVELFSLSRQVAR 352          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Celegans
Match: mab-10 (pep chromosome:WBcel235:II:10528084:10533880:1 gene:WBGene00003107.1 transcript:R166.1a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mab-10)

HSP 1 Score: 89.3521 bits (220), Expect = 9.494e-19
Identity = 51/122 (41.80%), Postives = 73/122 (59.84%), Query Frame = 2
Query:  593 SLLPNAVLSNSEKSKIKLAAENLAQTLPKIDARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVKMA 958
            SL  N  LS ++ S++   A   ++ LP +  R+    +K V+K ++ +L      P   +   +YSAIYGRF+  R+  K +  HE +VNEAAAQL L +P L+TRRD LF LARQ+VK A
Sbjct:  389 SLTENPTLSTAQISRLAECALAASKNLPPLPPRL-VQNKKRVSKEVIELLKCSPATPSMIHAFRKYSAIYGRFDTKRKPHKVLTLHETTVNEAAAQLCLLVPSLLTRRDELFPLARQIVKDA 509          

HSP 2 Score: 81.6481 bits (200), Expect = 2.788e-16
Identity = 37/83 (44.58%), Postives = 58/83 (69.88%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIP 304
            +E +L  +L +ANL QY+  F+A+GGDD++Q+    + EF EI+ LVGM  KPLH+RR+Q+A+ E+S++   FN +  + I P
Sbjct:   88 SEWQLLAVLSKANLVQYYDVFIAQGGDDINQIMACEEREFLEIMNLVGMLPKPLHVRRMQRALAEYSQDQTAFNLAALQQIGP 170          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Fly
Match: nab (gene:FBgn0259986 transcript:FBtr0332660)

HSP 1 Score: 102.449 bits (254), Expect = 7.333e-23
Identity = 55/118 (46.61%), Postives = 77/118 (65.25%), Query Frame = 2
Query:  611 VLSNSEKSKIKLAAENLAQTLPKIDARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSL--QIPELITRRDNLFHLARQVVKMA 958
            VL+ ++  +I + A+ + + LP+ + R  +T RK   + L  +++M E DP R ++I +YSAIYGRF+  RR  KP+  HEV VNEAAAQL    Q   L+TRRD LF LARQ+VK A
Sbjct:  309 VLTEAQIQRITMCADKIGRQLPQREPRAQTT-RKRTTRELEQVIAMGEQDPRRMDEIRKYSAIYGRFDCKRRPEKPLTLHEVCVNEAAAQLCRNPQTIWLLTRRDELFPLARQIVKDA 425          

HSP 2 Score: 96.2857 bits (238), Expect = 8.064e-21
Identity = 43/72 (59.72%), Postives = 54/72 (75.00%), Query Frame = 2
Query:   59 EVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEF 274
            EV+LYR+L+RA+L  Y+   L  GGDDV QL +A +EEF EI+ LVGM  KPLH+RRLQKA+ EW+ NP  F
Sbjct:  118 EVQLYRVLQRASLLAYYDTLLEMGGDDVQQLYDAGEEEFLEIMALVGMASKPLHVRRLQKALHEWANNPGLF 189          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Fly
Match: nab (gene:FBgn0259986 transcript:FBtr0302032)

HSP 1 Score: 102.449 bits (254), Expect = 7.606e-23
Identity = 55/118 (46.61%), Postives = 77/118 (65.25%), Query Frame = 2
Query:  611 VLSNSEKSKIKLAAENLAQTLPKIDARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSL--QIPELITRRDNLFHLARQVVKMA 958
            VL+ ++  +I + A+ + + LP+ + R  +T RK   + L  +++M E DP R ++I +YSAIYGRF+  RR  KP+  HEV VNEAAAQL    Q   L+TRRD LF LARQ+VK A
Sbjct:  309 VLTEAQIQRITMCADKIGRQLPQREPRAQTT-RKRTTRELEQVIAMGEQDPRRMDEIRKYSAIYGRFDCKRRPEKPLTLHEVCVNEAAAQLCRNPQTIWLLTRRDELFPLARQIVKDA 425          

HSP 2 Score: 95.9005 bits (237), Expect = 9.097e-21
Identity = 43/73 (58.90%), Postives = 54/73 (73.97%), Query Frame = 2
Query:   59 EVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFN 277
            EV+LYR+L+RA+L  Y+   L  GGDDV QL +A +EEF EI+ LVGM  KPLH+RRLQKA+ EW+ NP  F 
Sbjct:  118 EVQLYRVLQRASLLAYYDTLLEMGGDDVQQLYDAGEEEFLEIMALVGMASKPLHVRRLQKALHEWANNPGLFQ 190          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Fly
Match: nab (gene:FBgn0259986 transcript:FBtr0302033)

HSP 1 Score: 102.064 bits (253), Expect = 1.103e-22
Identity = 55/118 (46.61%), Postives = 77/118 (65.25%), Query Frame = 2
Query:  611 VLSNSEKSKIKLAAENLAQTLPKIDARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSL--QIPELITRRDNLFHLARQVVKMA 958
            VL+ ++  +I + A+ + + LP+ + R  +T RK   + L  +++M E DP R ++I +YSAIYGRF+  RR  KP+  HEV VNEAAAQL    Q   L+TRRD LF LARQ+VK A
Sbjct:  309 VLTEAQIQRITMCADKIGRQLPQREPRAQTT-RKRTTRELEQVIAMGEQDPRRMDEIRKYSAIYGRFDCKRRPEKPLTLHEVCVNEAAAQLCRNPQTIWLLTRRDELFPLARQIVKDA 425          

HSP 2 Score: 96.6709 bits (239), Expect = 6.259e-21
Identity = 43/72 (59.72%), Postives = 54/72 (75.00%), Query Frame = 2
Query:   59 EVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEF 274
            EV+LYR+L+RA+L  Y+   L  GGDDV QL +A +EEF EI+ LVGM  KPLH+RRLQKA+ EW+ NP  F
Sbjct:  118 EVQLYRVLQRASLLAYYDTLLEMGGDDVQQLYDAGEEEFLEIMALVGMASKPLHVRRLQKALHEWANNPGLF 189          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Zebrafish
Match: nab1b (NGFI-A binding protein 1b (EGR1 binding protein 1) [Source:ZFIN;Acc:ZDB-GENE-081105-85])

HSP 1 Score: 180.259 bits (456), Expect = 2.187e-50
Identity = 123/319 (38.56%), Postives = 174/319 (54.55%), Query Frame = 2
Query:   29 MALTSNLCSNEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLK---ANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLMNL--PSFFNSSVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKLAA---ENLAQTLPKID---ARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
            MA T      E++LYR+L+RANL  Y+  F+ +GGDDV QL EA ++EF EI+ LVGM  KPLH+RRLQKA+R+W  NP  FN+  T   +P+ + P  K    +  L+P +   R + ++   K    +C D      +P        N  +  TVS L +   P F+ +    +  +     L    G P S  P   L   + + ++  A   E + QTLPK D    +    G K + K +  I  M E DP RE +I +YSAIYGRF++ R+ GK +  HE++VNEAAAQL ++   L+TRRD LF LARQV +
Sbjct:    1 MAATLPRTLGELQLYRILQRANLLCYYDAFIQQGGDDVQQLCEAGEDEFLEIMALVGMASKPLHVRRLQKALRDWVTNPALFNQPLTS--LPVCSIPVYKLPEGSPTLLPGNG-ARGDTQVKVPKCLAASCVDPG--KGEP-------GNGNSSATVSPLQSGSEPRFWANHGTESEHSLSPAEL----GSPSS--PRDGLEALDAAAVQSVAECVERITQTLPKSDPTEVKDQLKGNKKLAKMISHIFDMSEEDPHREEEIRKYSAIYGRFDSKRKDGKHLTLHELTVNEAAAQLCMKEVALLTRRDELFGLARQVSR 301          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Zebrafish
Match: nab1a (NGFI-A binding protein 1a (EGR1 binding protein 1) [Source:ZFIN;Acc:ZDB-GENE-050208-574])

HSP 1 Score: 164.466 bits (415), Expect = 1.002e-44
Identity = 107/307 (34.85%), Postives = 162/307 (52.77%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETI--NTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKL---AAENLAQTLPKID---ARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
             E++LYR+L++ANL  Y+  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+R+W  NP  FN+       PL++ P        +P  KL      + +      N   + + + + LLP         +  +       L     S  + +     +     CS  P S  P+  L   + + ++      E L+ TL K D    + +    K ++K +  I  M + DP +E +I +YSAIYGRF++ RR G+ +  HE++VNEA+AQL ++   L+TRRD LF LARQ+ +
Sbjct:   10 GELQLYRILQKANLLCYYEAFIQQGGDDVQQLCEAGEEEFLEIMALVGMATKPLHVRRLQKALRDWVTNPSLFNQ-------PLTSLPVCS-----IPVYKLPESSPGVHS------NGQQNQLVVPRALLPTSPSPGRLDLSRDGTPGCSPLQGASESRFWGSESEHSLSPSGACS--PCS--PHDALDTLDSAGVQCVMECVERLSHTLSKSDPTEVKEHIRTNKKLSKMIGHICEMSDDDPQKEVEIRKYSAIYGRFDSKRRDGRQLTMHELTVNEASAQLCIKDLSLLTRRDELFSLARQISR 294          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Zebrafish
Match: nab2 (NGFI-A binding protein 2 (EGR1 binding protein 2) [Source:ZFIN;Acc:ZDB-GENE-080204-105])

HSP 1 Score: 164.466 bits (415), Expect = 1.437e-44
Identity = 114/368 (30.98%), Postives = 179/368 (48.64%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIP------------------LSARPYLKANQNLVPCDKLKRLENEITNFK--TEDYNCFDSNIFMSQPLLPLPDDEN--NETINTVSNLMNLPSFFNSS-------------VFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLPKIDA-------RINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVKMAEEIKNDNFKLPHNHYDDEMKHNVDC 1033
             E++LYR+L+RANL  Y+  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+R+W+ NP  FN+  T  + P                  + AR  +   Q   PC++ + + + +T  +  +    C  ++        P+P D +   E ++ + +    P  + S+                + + G        S   VS  P   L       ++ + + L +T+P+ D        R+N    K + K +  I ++   D  +E +I +YS IYGRF++ RR GK + HHE+ +NEAAAQ  ++   L+ RR  LF LARQV +  E      FK  H  Y ++   + +C
Sbjct:    8 GELQLYRVLQRANLLMYYDTFIQQGGDDVQQLCEAGEEEFLEIMALVGMATKPLHVRRLQKALRDWASNPTLFNQPLTTNVAPCGVPLLRIDSAGSANSTIMGARKSMSNGQPGSPCER-EEVGSCLTPLRENSPKSPCSQAS--------PIPPDHHLYREKLSPMDSHWLSPEIYGSADLDEDQPVPPLPFACHSRLLGPPTPTSSSSSTLVSPWPGGQLDPDTIKAVEDSVDRLLRTMPQADQTEVKTLLRLN----KKMAKTVGHIFNLEAQDKSKEEEIRKYSLIYGRFDSKRREGKQLTHHEMLINEAAAQFCIRDNALLLRRVELFSLARQVAR--ECAYTSTFKH-HRTYSEDCSASAEC 359          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Xenopus
Match: nab1 (NGFI-A binding protein 1 [Source:NCBI gene;Acc:100145272])

HSP 1 Score: 168.703 bits (426), Expect = 2.473e-46
Identity = 117/311 (37.62%), Postives = 168/311 (54.02%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFM----SQPLLPLPDD-ENNETINTVSNLMNLPSFFNS----SVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLPKID---ARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
             E++LYR+L+RANL  YF  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+R+W  NP  FN+  T   +P+S+ P  K  ++      L  + +  +  K+   N  D ++ +    +  L P   D   N  +   S   +  S   +    S+  A +       DI   +  S    A LS +E        E +  +LPK D    R      K ++K +  I  M + DP RE +I +YSAIYGRF++ R+ GK +  HE++VNEAAAQL ++   L+TRRD LF LARQ+ +
Sbjct:   10 GELQLYRILQRANLLSYFDAFIQQGGDDVQQLCEAGEEEFLEIMALVGMASKPLHVRRLQKALRDWVTNPGIFNQPLTS--LPVSSIPIYKLPES---SSALLGINSNSSEMKS---NTRDPHLKIPKCAATTLCPGKSDVVGNSPLQNNSEGRHWQSHHTTESEHSLSPADLGSPASPRDITETLDTS----AALSVAE------CVERMFPSLPKSDVNEVREFLKTNKKLSKMIGHIFEMVDEDPHREEEIRKYSAIYGRFDSKRKDGKHLTLHELTVNEAAAQLCMKDIALLTRRDELFTLARQISR 302          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Xenopus
Match: chd3 (chromodomain helicase DNA binding protein 3 [Source:Xenbase;Acc:XB-GENE-1008908])

HSP 1 Score: 145.976 bits (367), Expect = 7.892e-38
Identity = 105/323 (32.51%), Postives = 161/323 (49.85%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKLA------AENLAQTLPKID---ARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVKMAEEIKNDNFKLPHN 997
             E++LYR+L+RANL  Y+  F+ +GGDDV QL EA ++EF EI+ LVGM  KPLH+RRLQKA+R+W+ NP  FN+     I   S+ P  K ++ +    ++ R  N   +    +  C     F+          E  E I+        PS  +   ++A ++  +   D           +   S  E+ + +LA       + L + LP+ D   AR      K + + L  I  M      +E +I R+SAIYGR  + RR GK +  HE+++NEAAAQ  ++   L+ RR  LF LARQ  + +  + +     P+N
Sbjct:   38 GELQLYRVLQRANLLGYYDTFIQQGGDDVQQLCEAGEDEFLEIMSLVGMATKPLHVRRLQKALRDWATNPGLFNQPALGNIPVSSSIPLFKISETV---GRIGR--NTNGHLNVPEVQCKSPGNFVL---------EQAEKIS--------PSQLDRRQWTAQISPELDDADEEEVGEEENGGDQYSSPGERLEPELAQVVSDSVDRLMKGLPRGDISEARSMLKLNKKLGRSLGHIFQMGNGSQRKEREIRRHSAIYGRSESKRREGKRLTLHELTINEAAAQCCMRDNTLLLRRVELFSLARQAARESSYLASLKCSRPNN 338          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Xenopus
Match: chd3 (chromodomain helicase DNA binding protein 3 [Source:Xenbase;Acc:XB-GENE-1008908])

HSP 1 Score: 145.591 bits (366), Expect = 7.945e-38
Identity = 103/308 (33.44%), Postives = 155/308 (50.32%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKLA------AENLAQTLPKID---ARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
             E++LYR+L+RANL  Y+  F+ +GGDDV QL EA ++EF EI+ LVGM  KPLH+RRLQKA+R+W+ NP  FN+     I   S+ P  K ++ +    ++ R  N   +    +  C     F+          E  E I+        PS  +   ++A ++  +   D           +   S  E+ + +LA       + L + LP+ D   AR      K + + L  I  M      +E +I R+SAIYGR  + RR GK +  HE+++NEAAAQ  ++   L+ RR  LF LARQ  +
Sbjct:   16 GELQLYRVLQRANLLGYYDTFIQQGGDDVQQLCEAGEDEFLEIMSLVGMATKPLHVRRLQKALRDWATNPGLFNQPALGNIPVSSSIPLFKISETV---GRIGR--NTNGHLNVPEVQCKSPGNFVL---------EQAEKIS--------PSQLDRRQWTAQISPELDDADEEEVGEEENGGDQYSSPGERLEPELAQVVSDSVDRLMKGLPRGDISEARSMLKLNKKLGRSLGHIFQMGNGSQRKEREIRRHSAIYGRSESKRREGKRLTLHELTINEAAAQCCMRDNTLLLRRVELFSLARQAAR 301          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Xenopus
Match: chd3 (chromodomain helicase DNA binding protein 3 [Source:Xenbase;Acc:XB-GENE-1008908])

HSP 1 Score: 145.976 bits (367), Expect = 9.965e-38
Identity = 105/323 (32.51%), Postives = 161/323 (49.85%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKLA------AENLAQTLPKID---ARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVKMAEEIKNDNFKLPHN 997
             E++LYR+L+RANL  Y+  F+ +GGDDV QL EA ++EF EI+ LVGM  KPLH+RRLQKA+R+W+ NP  FN+     I   S+ P  K ++ +    ++ R  N   +    +  C     F+          E  E I+        PS  +   ++A ++  +   D           +   S  E+ + +LA       + L + LP+ D   AR      K + + L  I  M      +E +I R+SAIYGR  + RR GK +  HE+++NEAAAQ  ++   L+ RR  LF LARQ  + +  + +     P+N
Sbjct:   54 GELQLYRVLQRANLLGYYDTFIQQGGDDVQQLCEAGEDEFLEIMSLVGMATKPLHVRRLQKALRDWATNPGLFNQPALGNIPVSSSIPLFKISETV---GRIGR--NTNGHLNVPEVQCKSPGNFVL---------EQAEKIS--------PSQLDRRQWTAQISPELDDADEEEVGEEENGGDQYSSPGERLEPELAQVVSDSVDRLMKGLPRGDISEARSMLKLNKKLGRSLGHIFQMGNGSQRKEREIRRHSAIYGRSESKRREGKRLTLHELTINEAAAQCCMRDNTLLLRRVELFSLARQAARESSYLASLKCSRPNN 354          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Mouse
Match: Nab1 (Ngfi-A binding protein 1 [Source:MGI Symbol;Acc:MGI:107564])

HSP 1 Score: 166.392 bits (420), Expect = 1.489e-45
Identity = 111/313 (35.46%), Postives = 167/313 (53.35%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVS------NLMNLPSFFNSSVF----SAPMNGRVKSLDICSGIPVSLLPNA-VLSNSEKSKIKLAAENLAQTLPKID-ARINSTGR--KDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
             E++LYR+L++ANL  YF  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+R+W  NP  FN+  T   +P+S+ P  K             L      +     N ++ +    +P L +P       + ++         + L S  +S ++    +      +   D+  G P S   ++  L  +    +    E +A TLPK D + +    +  K + K +  I  M + DP +E +I +YSAIYGRF++ R+ GK +  HE++VNEAAAQL ++   L+TRRD LF LARQV +
Sbjct:   10 GELQLYRILQKANLLSYFDAFIQQGGDDVQQLCEAGEEEFLEIMALVGMASKPLHVRRLQKALRDWVTNPGLFNQPLTS--LPVSSIPIYK-------------LPEGSPTWLGISCNSYERSSSSREPHLKIPKCAATTCVQSLGQGKSEVGSLALQSVSDSRLWQGHHATESEHSLSPADL--GSPASPKESSEALDAAAALSVAECVERMAPTLPKSDLSEVKELLKNNKKLAKMIGHIFEMSDEDPHKEEEIRKYSAIYGRFDSKRKDGKHLTLHELTVNEAAAQLCVKDNALLTRRDELFALARQVSR 305          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Mouse
Match: Nab1 (Ngfi-A binding protein 1 [Source:MGI Symbol;Acc:MGI:107564])

HSP 1 Score: 166.392 bits (420), Expect = 2.198e-45
Identity = 116/314 (36.94%), Postives = 165/314 (52.55%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICSGIPVSLLP---NAVLSNSEKSKIKLAA---------ENLAQTLPKID-ARINSTGR--KDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
             E++LYR+L++ANL  YF  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+R+W  NP  FN+  T   +P+S+ P  K             L      +     N ++ +    +P L +P          V +L    S   S    +  + R+      +    SL P    +  S  E S+   AA         E +A TLPK D + +    +  K + K +  I  M + DP +E +I +YSAIYGRF++ R+ GK +  HE++VNEAAAQL ++   L+TRRD LF LARQV +
Sbjct:   10 GELQLYRILQKANLLSYFDAFIQQGGDDVQQLCEAGEEEFLEIMALVGMASKPLHVRRLQKALRDWVTNPGLFNQPLTS--LPVSSIPIYK-------------LPEGSPTWLGISCNSYERSSSSREPHLKIP---KCAATTCVQSLGQGKSEVGSLALQSVSDSRLWQGHHATESEHSLSPADLGSPASPKESSEALDAAAALSVAECVERMAPTLPKSDLSEVKELLKNNKKLAKMIGHIFEMSDEDPHKEEEIRKYSAIYGRFDSKRKDGKHLTLHELTVNEAAAQLCVKDNALLTRRDELFALARQVSR 305          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Mouse
Match: Nab1 (Ngfi-A binding protein 1 [Source:MGI Symbol;Acc:MGI:107564])

HSP 1 Score: 166.392 bits (420), Expect = 2.249e-45
Identity = 116/314 (36.94%), Postives = 165/314 (52.55%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICSGIPVSLLP---NAVLSNSEKSKIKLAA---------ENLAQTLPKID-ARINSTGR--KDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
             E++LYR+L++ANL  YF  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+R+W  NP  FN+  T   +P+S+ P  K             L      +     N ++ +    +P L +P          V +L    S   S    +  + R+      +    SL P    +  S  E S+   AA         E +A TLPK D + +    +  K + K +  I  M + DP +E +I +YSAIYGRF++ R+ GK +  HE++VNEAAAQL ++   L+TRRD LF LARQV +
Sbjct:   10 GELQLYRILQKANLLSYFDAFIQQGGDDVQQLCEAGEEEFLEIMALVGMASKPLHVRRLQKALRDWVTNPGLFNQPLTS--LPVSSIPIYK-------------LPEGSPTWLGISCNSYERSSSSREPHLKIP---KCAATTCVQSLGQGKSEVGSLALQSVSDSRLWQGHHATESEHSLSPADLGSPASPKESSEALDAAAALSVAECVERMAPTLPKSDLSEVKELLKNNKKLAKMIGHIFEMSDEDPHKEEEIRKYSAIYGRFDSKRKDGKHLTLHELTVNEAAAQLCVKDNALLTRRDELFALARQVSR 305          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Mouse
Match: Nab2 (Ngfi-A binding protein 2 [Source:MGI Symbol;Acc:MGI:107563])

HSP 1 Score: 151.369 bits (381), Expect = 5.180e-40
Identity = 110/324 (33.95%), Postives = 161/324 (49.69%), Query Frame = 2
Query:   29 MALTSNLCSNEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFD--SNIFMSQPLLPLPDDENNETINTVSNLMNLPSFF--------NSSVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKLAAENLA---QTLPKID-ARINSTGR--KDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
            MAL   L   E++LYR+L+RANL  Y+  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+REW+ NP  F  S+    +P+S+ P  K ++            +     K      F   S + + + L PLP                  S           S  FS P  G V S     G   +          E   +++  E++    ++ P+ D   I S  +  K + + +  I  M +HD  +E +I +YS IYGR ++ RR GK ++ HE+++NEAAAQ  ++   L+ RR  LF L+RQV +
Sbjct:   34 MALPRTL--GELQLYRVLQRANLLSYYETFIQQGGDDVQQLCEAGEEEFLEIMALVGMATKPLHVRRLQKALREWATNPGLF--SQPVPAVPVSSIPLFKISETAGTRKGSMSNGHGSPGEKAGSARSFSPKSPLELGEKLSPLPGGPGAGDPRIWPGQSTPESDVGAGGEEEAGSPPFSPPAGGGV-SEGPGVGGVAAGGAGGGPDRLEPEMVRMVVESVERIFRSFPRGDTGEIASLLKLNKKLARSVGHIFEMDDHDAQKEEEIRKYSVIYGRLDSKRREGKQLSLHELTINEAAAQFCMRDNTLLLRRVELFSLSRQVAR 352          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Mouse
Match: Nab2 (Ngfi-A binding protein 2 [Source:MGI Symbol;Acc:MGI:107563])

HSP 1 Score: 150.984 bits (380), Expect = 1.863e-39
Identity = 109/324 (33.64%), Postives = 161/324 (49.69%), Query Frame = 2
Query:   29 MALTSNLCSNEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFD--SNIFMSQPLLPLPDDENNETINTVSNLMNLPSFF--------NSSVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKLAAENLA---QTLPKID-ARINSTGR--KDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
            MAL   L   E++LYR+L+RANL  Y+  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+REW+ NP  F  S+    +P+S+ P  K ++            +     K      F   S + + + L PLP                  S           S  FS P  G V       G+  +          E   +++  E++    ++ P+ D   I S  +  K + + +  I  M +HD  +E +I +YS IYGR ++ RR GK ++ HE+++NEAAAQ  ++   L+ RR  LF L+RQV +
Sbjct:   34 MALPRTL--GELQLYRVLQRANLLSYYETFIQQGGDDVQQLCEAGEEEFLEIMALVGMATKPLHVRRLQKALREWATNPGLF--SQPVPAVPVSSIPLFKISETAGTRKGSMSNGHGSPGEKAGSARSFSPKSPLELGEKLSPLPGGPGAGDPRIWPGQSTPESDVGAGGEEEAGSPPFSPPAGGGVSEGPGVGGV-AAGGAGGGPDRLEPEMVRMVVESVERIFRSFPRGDTGEIASLLKLNKKLARSVGHIFEMDDHDAQKEEEIRKYSVIYGRLDSKRREGKQLSLHELTINEAAAQFCMRDNTLLLRRVELFSLSRQVAR 352          
BLAST of NGFI-A-binding protein 1 vs. UniProt/SwissProt
Match: sp|O35589|NAB1_MESAU (NGFI-A-binding protein 1 OS=Mesocricetus auratus OX=10036 GN=NAB1 PE=2 SV=1)

HSP 1 Score: 172.94 bits (437), Expect = 7.790e-47
Identity = 119/316 (37.66%), Postives = 164/316 (51.90%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNS---EKSKIKLAA---------ENLAQTLPKIDARINSTGR-----KDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
             E++LYR+L++ANL  YF  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+R+W  NP  FN+  T   +P+S+ P  K             L      +     N ++ N    +P L +P          V +L    S   S    +    R+      +    SL P  V S +   E S+   AA         E +A TLPK D  +N         K + K +  I  M + DP +E +I +YSAIYGRF++ R+ GK +  HE++VNEAAAQL ++   L+TRRD LF LARQV +
Sbjct:   10 GELQLYRILQKANLLSYFGAFIQQGGDDVQQLCEAGEEEFLEIMALVGMASKPLHVRRLQKALRDWVTNPGLFNQPLTS--LPVSSIPIYK-------------LPKGSPTWLGISCNSYERNSSAREPHLKIP---KCAATTCVQSLGQGKSEVGSLAVQSVSESRLWQGHHATESEHSLSPADVGSPASPKESSEALDAAAALSVAECVERMASTLPKSD--LNEVKELLKNNKKLAKMIGHIFEMSDEDPHKEEEIRKYSAIYGRFDSKRKDGKHLTLHELTVNEAAAQLCVKDNALLTRRDELFALARQVSR 305          
BLAST of NGFI-A-binding protein 1 vs. UniProt/SwissProt
Match: sp|Q62722|NAB1_RAT (NGFI-A-binding protein 1 OS=Rattus norvegicus OX=10116 GN=Nab1 PE=2 SV=2)

HSP 1 Score: 169.859 bits (429), Expect = 9.983e-46
Identity = 113/313 (36.10%), Postives = 164/313 (52.40%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVS------NLMNLPSFFNSSVFSAPMNGRVK-SLDICS-GIPVSLLPNA-VLSNSEKSKIKLAAENLAQTLPKIDARINSTGR-----KDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
             E++LYR+L++ANL  YF  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+R+W  NP  FN+  T   +P+S+ P  K             L      +     N ++ +    +P L +P       + ++         + L S   S ++        + SL     G P S   ++  L  +    +    E +A TLPK D  +N         K + K +  I  M + DP +E +I +YSAIYGRF++ R+ GK +  HE++VNEAAAQL ++   L+TRRD LF LARQV +
Sbjct:   10 GELQLYRILQKANLLSYFDAFIQQGGDDVQQLCEAGEEEFLEIMALVGMASKPLHVRRLQKALRDWVTNPGLFNQPLTS--LPVSSIPIYK-------------LPEGSPTWLGISCNSYERSSSAREPHLKVPKCAATTCVQSLGQGKSEVGSLALQSVSESRLWQGHHTTESEHSLSPADLGSPASPKESSEALDAAAALSVAECVERMAPTLPKSD--LNEVKELLKNNKKLAKMIGHIFEMSDEDPHKEEEIRKYSAIYGRFDSKRKDGKHLTLHELTVNEAAAQLCVKDNALLTRRDELFALARQVSR 305          
BLAST of NGFI-A-binding protein 1 vs. UniProt/SwissProt
Match: sp|Q13506|NAB1_HUMAN (NGFI-A-binding protein 1 OS=Homo sapiens OX=9606 GN=NAB1 PE=1 SV=2)

HSP 1 Score: 166.777 bits (421), Expect = 1.399e-44
Identity = 113/321 (35.20%), Postives = 165/321 (51.40%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNL-----VPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINT-------VSNLMNLPSFFNSSVF----SAPMNGRVKSLDICSGIPVSLLPNA-VLSNSEKSKIKLAAENLAQTLPKIDARINSTGR-----KDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
             E++LYR+L++ANL  YF  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+R+W  NP  FN+  T   +P+S+ P  K  +       + C   +R  N                    +P L +P       + +       V   + L S   S ++    +      +   D+  G P S   ++  L  +    +    E +A TLPK D  +N         K + K +  I  M + DP +E +I +YSAIYGRF++ R+ GK +  HE++VNEAAAQL ++   L+TRRD LF LARQ+ +
Sbjct:   10 GELQLYRILQKANLLSYFDAFIQQGGDDVQQLCEAGEEEFLEIMALVGMASKPLHVRRLQKALRDWVTNPGLFNQPLTS--LPVSSIPIYKLPEGSPTWLGISCSSYERSSN------------------AREPHLKIPKCAATTCVQSLGQGKSDVVGSLALQSVGESRLWQGHHATESEHSLSPADL--GSPASPKESSEALDAAAALSVAECVERMAPTLPKSD--LNEVKELLKTNKKLAKMIGHIFEMNDDDPHKEEEIRKYSAIYGRFDSKRKDGKHLTLHELTVNEAAAQLCVKDNALLTRRDELFALARQISR 306          
BLAST of NGFI-A-binding protein 1 vs. UniProt/SwissProt
Match: sp|Q61122|NAB1_MOUSE (NGFI-A-binding protein 1 OS=Mus musculus OX=10090 GN=Nab1 PE=1 SV=2)

HSP 1 Score: 166.392 bits (420), Expect = 1.537e-44
Identity = 116/314 (36.94%), Postives = 165/314 (52.55%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICSGIPVSLLP---NAVLSNSEKSKIKLAA---------ENLAQTLPKID-ARINSTGR--KDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
             E++LYR+L++ANL  YF  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+R+W  NP  FN+  T   +P+S+ P  K             L      +     N ++ +    +P L +P          V +L    S   S    +  + R+      +    SL P    +  S  E S+   AA         E +A TLPK D + +    +  K + K +  I  M + DP +E +I +YSAIYGRF++ R+ GK +  HE++VNEAAAQL ++   L+TRRD LF LARQV +
Sbjct:   10 GELQLYRILQKANLLSYFDAFIQQGGDDVQQLCEAGEEEFLEIMALVGMASKPLHVRRLQKALRDWVTNPGLFNQPLTS--LPVSSIPIYK-------------LPEGSPTWLGISCNSYERSSSSREPHLKIP---KCAATTCVQSLGQGKSEVGSLALQSVSDSRLWQGHHATESEHSLSPADLGSPASPKESSEALDAAAALSVAECVERMAPTLPKSDLSEVKELLKNNKKLAKMIGHIFEMSDEDPHKEEEIRKYSAIYGRFDSKRKDGKHLTLHELTVNEAAAQLCVKDNALLTRRDELFALARQVSR 305          
BLAST of NGFI-A-binding protein 1 vs. UniProt/SwissProt
Match: sp|Q15742|NAB2_HUMAN (NGFI-A-binding protein 2 OS=Homo sapiens OX=9606 GN=NAB2 PE=1 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 8.343e-39
Identity = 113/329 (34.35%), Postives = 163/329 (49.54%), Query Frame = 2
Query:   29 MALTSNLCSNEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFD--SNIFMSQPLLPLPDDENNETINTVSNLMNLPSFF--------NSSVFSAPMNGRVK--------SLDICSGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLPKIDA-RINSTGR--KDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
            MAL   L   E++LYR+L+RANL  Y+  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+REW+ NP  F  S+    +P+S+ P  K ++            +     K      F   S + + + L PLP                  S           S  FS P  G V         +     G P  L P  V    E      + E + ++ P+ DA  + S  +  K + + +  I  M ++D  +E +I +YS IYGRF++ RR GK ++ HE+++NEAAAQ  ++   L+ RR  LF L+RQV +
Sbjct:   34 MALPRTL--GELQLYRVLQRANLLSYYETFIQQGGDDVQQLCEAGEEEFLEIMALVGMATKPLHVRRLQKALREWATNPGLF--SQPVPAVPVSSIPLFKISETAGTRKGSMSNGHGSPGEKAGSARSFSPKSPLELGEKLSPLPGGPGAGDPRIWPGRSTPESDVGAGGEEEAGSPPFSPPAGGGVPEGTGAGGLAAGGTGGGPDRLEPEMVRMVVE------SVERIFRSFPRGDAGEVTSLLKLNKKLARSVGHIFEMDDNDSQKEEEIRKYSIIYGRFDSKRREGKQLSLHELTINEAAAQFCMRDNTLLLRRVELFSLSRQVAR 352          
BLAST of NGFI-A-binding protein 1 vs. TrEMBL
Match: A0A2L2YVQ2 (NGFI-A-binding protein-like protein (Fragment) OS=Parasteatoda tepidariorum OX=114398 PE=2 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 7.917e-52
Identity = 118/303 (38.94%), Postives = 166/303 (54.79%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLP-----DDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLPKIDARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVV 949
            +E +LYR+L+RANL  Y+  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA++EW  NP  F        +P S  PY  +   + P +  +                  S +   QP  P+P     + +++ +          P+ F  ++   P            G P SL P  VL  S+  ++  AA+ L +TLP  D +  +  +K + K L  +++MPE DP R ++I +Y+AIYGRF+  R+  KP+  HEVSVNEAAAQ+   +P LITRRD LF LARQVV
Sbjct:   72 SEPQLYRVLQRANLLSYYDTFICQGGDDVQQLCEAGEEEFLEIMALVGMASKPLHVRRLQKALQEWVNNPAMFQTPLLPSSVPQS--PYPVSPPVVRPLNLSQ-----------------PSPVAPMQPSPPMPILSNSNSQSSPSPGAKEASSPQPTSFQQALGEYPP---------APGSPTSLTP--VLVESQIQRLAEAADLLVKTLPPFDPKPQN-NKKKICKDLEYVMNMPEDDPRRMDEIRKYAAIYGRFDCKRKPEKPLTLHEVSVNEAAAQICKHVPALITRRDELFPLARQVV 343          
BLAST of NGFI-A-binding protein 1 vs. TrEMBL
Match: A0A1B6LVR5 (Uncharacterized protein (Fragment) OS=Graphocephala atropunctata OX=36148 GN=g.19839 PE=4 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 8.262e-52
Identity = 121/300 (40.33%), Postives = 164/300 (54.67%), Query Frame = 2
Query:   59 EVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDI--CSGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLPKIDARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
            E++LYR+++RA+L  Y+   L  GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA++EW  NP  F         PL    +   +  +VP                   +   +    +  L P P        NT   +  +PS   S     P  G   S       G P+ L P  VL  S+ S++  AA+NL ++LP ++ + N+T RK   K L ++LSM E DP R +QI +Y+AIYGRF+  R+  KP+  HEVSVNEAAAQ+   +P L+TRRD LF LARQVV+
Sbjct:   53 ELQLYRVMQRASLLAYYDTLLEMGGDDVQQLCEAGEEEFLEIMALVGMASKPLHVRRLQKALQEWLTNPAMFQ-------TPLLPGGF---SSRVVPAGPACVPPLSSPGPSPLRPDTAPTTTLCA--LSPSP--------NTTQGVGEVPSSAGS-----PSRGLTSSTSPGPSPGSPLQLTP--VLVPSQISRLADAAQNLIRSLPHLEPKANNTKRKAC-KDLETVLSMAEDDPLRMDQIRKYAAIYGRFDCKRKPEKPLTLHEVSVNEAAAQICRLVPALLTRRDELFPLARQVVR 324          
BLAST of NGFI-A-binding protein 1 vs. TrEMBL
Match: A0A1A9YIH5 (Uncharacterized protein OS=Glossina fuscipes fuscipes OX=201502 PE=4 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 2.921e-50
Identity = 122/332 (36.75%), Postives = 176/332 (53.01%), Query Frame = 2
Query:   59 EVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEF------------NESKTEIII------------------PLSARPYLKAN--QNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLPKIDARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVKMA 958
            EV+LYR+L++A+L  Y+   L  GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+ EW+ NP++F              +K+ II                   P +  P        N VP      L ++I +          S   +  P  PLP++ ++  + +            +S  ++ +     S  + S  P  L P  VLS+ + ++I + AE +A  LP+ + R + T +K  ++ L  +++M EHDP R ++I +YSAIYGRF+  RR  KP+  HEV VNEAAAQL   +P L+TRRD LF LARQ+VK A
Sbjct:   23 EVQLYRVLQKASLLAYYDTLLEMGGDDVQQLYEAGEEEFLEIMALVGMASKPLHVRRLQKALHEWANNPQQFQTPVANNSGQFDTPAKSSIIFNTEPSPSPSHCGPRPKFPPFNPTPAFNPTPLMNTVPPSTSAHLLSQICSLPATIQP--PSGPLIVPPTGPLPNERSHPLLAS-----------QTSTTASGVTPSNTSHQVTSSSP-QLTP--VLSDMQVARITVGAEKIAAQLPQREPRAH-TSKKRTSRELEQVIAMSEHDPRRMDEIRKYSAIYGRFDCKRRPEKPLTLHEVCVNEAAAQLCRLVPALLTRRDELFPLARQIVKDA 337          
BLAST of NGFI-A-binding protein 1 vs. TrEMBL
Match: A0A1A9UG60 (Uncharacterized protein OS=Glossina austeni OX=7395 PE=4 SV=1)

HSP 1 Score: 190.274 bits (482), Expect = 1.101e-49
Identity = 126/337 (37.39%), Postives = 175/337 (51.93%), Query Frame = 2
Query:   59 EVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEF------------NESKTEIII------------------PLSARPYLKAN--QNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDE-----NNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLPKIDARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVKMA 958
            EV+LYR+L++A+L  Y+   L  GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+ EW+ NP++F              +K+ II                   P +  P        N VP      L ++I +          S   +  P  PLP++       ++T  T S +              P N    S  + S  P  L P  VLS+ + ++I + AE +A  LP+ + R + T +K  ++ L  +++M EHDP R ++I +YSAIYGRF+  RR  KP+  HEV VNEAAAQL   +P L+TRRD LF LARQ+VK A
Sbjct:   41 EVQLYRVLQKASLLAYYDTLLEMGGDDVQQLYEAGEEEFLEIMALVGMASKPLHVRRLQKALHEWANNPQQFQTPVANNSGQFDTPAKSSIIFNAEPSPSPSHCGPRPKFPPFNPTPAFNPTPLMNTVPPSTSAHLLSQICSLPATIQP--PSGPLIVPPTGPLPNERPHPLLASQTTTTASGV-------------TPSN---TSHQVTSSSP-QLTP--VLSDMQVARITVGAEKIAAQLPQREPRAH-TSKKRTSRELEQVIAMSEHDPRRMDEIRKYSAIYGRFDCKRRPEKPLTLHEVCVNEAAAQLCRLVPALLTRRDELFPLARQIVKDA 355          
BLAST of NGFI-A-binding protein 1 vs. TrEMBL
Match: A0A1A9WLK7 (Uncharacterized protein OS=Glossina brevipalpis OX=37001 PE=4 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 2.204e-49
Identity = 120/330 (36.36%), Postives = 174/330 (52.73%), Query Frame = 2
Query:   59 EVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEF------------NESKTEIII----------------PLSARPYLKAN--QNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLPKIDARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVKMA 958
            EV+LYR+L++A+L  Y+   L  GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+ EW+ NP++F              +K+ +I                 P +  P        N VP      L ++I +          S   +  P  PLP++  +  + +            ++  ++ +     S  + S  P  L P  VLS  + ++I + AE +A  LP+ + R + T +K  ++ L  +++M EHDP R ++I +YSAIYGRF+  RR  KP+  HEV VNEAAAQL   +P L+TRRD LF LARQ+VK A
Sbjct:  146 EVQLYRVLQKASLLAYYDTLLEMGGDDVQQLYEAGEEEFLEIMALVGMASKPLHVRRLQKALHEWASNPQQFQTPITNNSGQFDTPAKSSVIFNTEPSPSHCAPRPKFPPFNPTPTFNPTPLMNTVPPSTSAHLLSQICSLPATIQP--PSGPLIVPPAGPLPNERPHPLLAS-----------QTTATASGVTHSSTSHQVTSSSP-QLTP--VLSEMQVARITVGAEKIAAQLPQREPRAH-TSKKRTSRELEQVIAMSEHDPRRMDEIRKYSAIYGRFDCKRRPEKPLTLHEVCVNEAAAQLCRLVPALLTRRDELFPLARQIVKDA 458          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Cavefish
Match: nab1b (NGFI-A binding protein 1 [Source:NCBI gene;Acc:103023089])

HSP 1 Score: 176.792 bits (447), Expect = 2.871e-49
Identity = 125/323 (38.70%), Postives = 172/323 (53.25%), Query Frame = 2
Query:   29 MALTSNLCSNEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLK---ANQNLVPCDKLKRLENEITNFKTEDYNCFD---------SNIFMSQPLLPLPDDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLPKID-ARINS--TGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
            MA T      E++LYR+L+RANL  Y+  F+ +GGDDV QL EA ++EF EI+ LVGM  KPLH+RRLQKA+R+W  NP  FN+  T   +P+ + P  K    +  L+P +   R E  +   K       D         S+   + PL    +        T S     P+   S   S+P +G +++LD            AV S +E        E +AQ LPK D A +     G K + K +  I  M E DP RE +I +YSAIYGRF++ R+ GK +  HE++VNEAAAQL ++   L+TRRD LF LARQV +
Sbjct:    1 MAATLPRTLGELQLYRILQRANLLCYYDAFIQQGGDDVQQLCEAGEDEFLEIMALVGMASKPLHVRRLQKALRDWVTNPALFNQPLTS--LPVCSIPVYKLPEGSPTLLPGNG-ARGETHVKVPKCVAAALVDPGKGEAGNGSSSATASPLQSGNEPRFWAGHGTESEHSLSPADLGSP--SSPRDG-LEALDTA----------AVQSVTE------CVERMAQALPKSDLAEVKEQLKGNKKLAKMISHIFDMSEEDPHREEEIRKYSAIYGRFDSKRKDGKHLTLHELTVNEAAAQLCMKEVALLTRRDELFGLARQVSR 301          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Cavefish
Match: nab1b (NGFI-A binding protein 1 [Source:NCBI gene;Acc:103023089])

HSP 1 Score: 176.792 bits (447), Expect = 3.595e-49
Identity = 125/330 (37.88%), Postives = 171/330 (51.82%), Query Frame = 2
Query:   29 MALTSNLCSNEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLK---ANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLM--NLPSFFNS--------------SVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLPKID-ARINS--TGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
            MA T      E++LYR+L+RANL  Y+  F+ +GGDDV QL EA ++EF EI+ LVGM  KPLH+RRLQKA+R+W  NP  FN+  T   +P+ + P  K    +  L+P +   R E  +   K       D             +  N  +  T S L   N P F+                   S+P +G +++LD            AV S +E        E +AQ LPK D A +     G K + K +  I  M E DP RE +I +YSAIYGRF++ R+ GK +  HE++VNEAAAQL ++   L+TRRD LF LARQV +
Sbjct:    1 MAATLPRTLGELQLYRILQRANLLCYYDAFIQQGGDDVQQLCEAGEDEFLEIMALVGMASKPLHVRRLQKALRDWVTNPALFNQPLTS--LPVCSIPVYKLPEGSPTLLPGNG-ARGETHVKVPKCVAAALVDPGKG---------EAGNGSSSATASPLQSGNEPRFWAGHGTESEHSLSPADLGSPSSPRDG-LEALDTA----------AVQSVTE------CVERMAQALPKSDLAEVKEQLKGNKKLAKMISHIFDMSEEDPHREEEIRKYSAIYGRFDSKRKDGKHLTLHELTVNEAAAQLCMKEVALLTRRDELFGLARQVSR 301          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Cavefish
Match: nab1a (NGFI-A-binding protein 1-like [Source:NCBI gene;Acc:103029816])

HSP 1 Score: 166.392 bits (420), Expect = 1.050e-45
Identity = 110/310 (35.48%), Postives = 162/310 (52.26%), Query Frame = 2
Query:   29 MALTSNLCSNEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDIC-SGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLP-KIDARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
            M+L       E++LYR+L+RANL  Y+  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+R+W  NP  F++       PL++ P        +P  KL+           ED     S    +QP  P              ++    +   +    +       +   C +G P  +L  AVL    +   +LA   L+++ P ++  R+   G K +++ +  +  + E DP RE +I +YSAIYGRF++ RR G+P+  HE+ VNEAAAQL L    L+T RD LF LAR + +
Sbjct:    1 MSLLMPRSVGELQLYRILQRANLLCYYAAFVQQGGDDVQQLCEAGEEEFLEIMALVGMATKPLHVRRLQKALRDWVTNPALFDQ-------PLTSLPACS-----IPVYKLR-----------EDSPTIYSTKQKTQPTEP-----------RAWSMSPSGAVGGAGTPGSESPSPSSACSPCWTGEPSEVLEPAVLHCVSECVRRLAP-TLSRSDPAEVKGRLR--GNKKLSQTITRLCELSEDDPQREEEIRKYSAIYGRFDSKRRDGRPLTTHELVVNEAAAQLCLLDGGLLTHRDQLFSLARHISR 273          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Cavefish
Match: nab1a (NGFI-A-binding protein 1-like [Source:NCBI gene;Acc:103029816])

HSP 1 Score: 167.162 bits (422), Expect = 1.087e-45
Identity = 108/301 (35.88%), Postives = 162/301 (53.82%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDIC-SGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLP-KIDARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
             E++LYR+L+RANL  Y+  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+R+W  NP  F++       PL++ P        +P  KL+  E+  T + T+           +QP  P              ++    +   +    +       +   C +G P  +L  AVL    +   +LA   L+++ P ++  R+   G K +++ +  +  + E DP RE +I +YSAIYGRF++ RR G+P+  HE+ VNEAAAQL L    L+T RD LF LAR + +
Sbjct:   10 GELQLYRILQRANLLCYYAAFVQQGGDDVQQLCEAGEEEFLEIMALVGMATKPLHVRRLQKALRDWVTNPALFDQ-------PLTSLPACS-----IPVYKLR--EDSPTIYSTKQ---------KTQPTEP-----------RAWSMSPSGAVGGAGTPGSESPSPSSACSPCWTGEPSEVLEPAVLHCVSECVRRLAP-TLSRSDPAEVKGRLR--GNKKLSQTITRLCELSEDDPQREEEIRKYSAIYGRFDSKRRDGRPLTTHELVVNEAAAQLCLLDGGLLTHRDQLFSLARHISR 273          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Cavefish
Match: nab1a (NGFI-A-binding protein 1-like [Source:NCBI gene;Acc:103029816])

HSP 1 Score: 167.162 bits (422), Expect = 1.087e-45
Identity = 108/301 (35.88%), Postives = 162/301 (53.82%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDIC-SGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLP-KIDARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
             E++LYR+L+RANL  Y+  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+R+W  NP  F++       PL++ P        +P  KL+  E+  T + T+           +QP  P              ++    +   +    +       +   C +G P  +L  AVL    +   +LA   L+++ P ++  R+   G K +++ +  +  + E DP RE +I +YSAIYGRF++ RR G+P+  HE+ VNEAAAQL L    L+T RD LF LAR + +
Sbjct:   10 GELQLYRILQRANLLCYYAAFVQQGGDDVQQLCEAGEEEFLEIMALVGMATKPLHVRRLQKALRDWVTNPALFDQ-------PLTSLPACS-----IPVYKLR--EDSPTIYSTKQ---------KTQPTEP-----------RAWSMSPSGAVGGAGTPGSESPSPSSACSPCWTGEPSEVLEPAVLHCVSECVRRLAP-TLSRSDPAEVKGRLR--GNKKLSQTITRLCELSEDDPQREEEIRKYSAIYGRFDSKRRDGRPLTTHELVVNEAAAQLCLLDGGLLTHRDQLFSLARHISR 273          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006363.1 (pep scaffold:Pmarinus_7.0:GL490594:2760:3775:-1 gene:ENSPMAG00000005754.1 transcript:ENSPMAT00000006363.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 110.923 bits (276), Expect = 2.685e-29
Identity = 52/96 (54.17%), Postives = 72/96 (75.00%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEI---IIPLSARPYLKAN 334
             E++L+R+L+RANL  Y+  F+A+GGDDV+QL EAS+EEF EI+ LVGM  KPLH+RRLQKA++EW  NP  F++  T++    IPL+  P L A+
Sbjct:   40 GELQLFRVLQRANLLSYYEPFVAQGGDDVAQLCEASEEEFLEIMALVGMASKPLHVRRLQKALQEWVSNPGGFSQPVTKLPAASIPLTRVPELSAS 135          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007439.1 (pep scaffold:Pmarinus_7.0:GL491332:428:5296:1 gene:ENSPMAG00000006717.1 transcript:ENSPMAT00000007439.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 83.1889 bits (204), Expect = 4.068e-18
Identity = 40/72 (55.56%), Postives = 52/72 (72.22%), Query Frame = 2
Query:  737 ILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
            I  M + D  RE++I ++SAIYGRF++ R+ GK +  HE+SVNEAA QL L    L+TRRD LF LARQVV+
Sbjct:  208 IFEMDDGDERREDEIRKFSAIYGRFDSRRKDGKQLTQHELSVNEAACQLCLLDMSLLTRRDELFPLARQVVR 279          

HSP 2 Score: 58.151 bits (139), Expect = 1.075e-9
Identity = 24/39 (61.54%), Postives = 31/39 (79.49%), Query Frame = 2
Query:  164 KEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNE 280
            +EEF EI+ LVGM  KPLH+RRLQKA++EW  NP  F++
Sbjct:    7 QEEFLEIMALVGMASKPLHVRRLQKALQEWVNNPSVFSQ 45          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Nematostella
Match: EDO41407 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4G0])

HSP 1 Score: 85.5001 bits (210), Expect = 9.312e-21
Identity = 36/65 (55.38%), Postives = 53/65 (81.54%), Query Frame = 2
Query:   59 EVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREW 253
            E+EL+ LL+ ANL  Y+ +F+ +GGDD+ QL +AS++EF EII +VGM+ KPLH+RRLQK++ +W
Sbjct:   11 ELELFTLLKSANLLDYYQSFIEQGGDDIQQLCDASEDEFKEIIAMVGMSTKPLHVRRLQKSLVDW 75          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Medaka
Match: nab1a (NGFI-A binding protein 1 [Source:NCBI gene;Acc:101175222])

HSP 1 Score: 167.162 bits (422), Expect = 1.288e-45
Identity = 115/308 (37.34%), Postives = 163/308 (52.92%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETI--NTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICS-GIPVSLLPNAVLSNSEKSKIKLAAE---NLAQTLPKID---ARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
             E++LYR+L+RANL  Y+  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+R+W  NP  FN+       PL++ P        +P  KL   E   T    +D     +++ + + +     D     +  + VS    L        +S   N    SL     G P S  P   L   + + ++   E    +A  LPK D    +      K + K +  I  M + DP RE++I +YSAIYGRF++ RR GK +  HE++VNEAAAQL ++   L+TRRD LF LARQ+ +
Sbjct:   10 GELQLYRILQRANLLYYYEAFIQQGGDDVQQLCEAGEEEFLEIMALVGMASKPLHVRRLQKALRDWVTNPAIFNQ-------PLTSLPVCS-----IPVYKLP--EGSPTLLNAQD-RANTASVKLPKAIAAACSDPGKLDVARDKVSAGSPLQGSSEVRFWSGHSNDSEHSLSPSDLGSPSS--PRDALEALDAAAVQSVLECVDRMAPGLPKTDLAEVKDQLKSNKKLAKMIGHIFEMSDDDPRREDEIRKYSAIYGRFDSKRRDGKHLTLHELTVNEAAAQLCMRDVALLTRRDELFGLARQISR 300          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Medaka
Match: nab1a (NGFI-A binding protein 1 [Source:NCBI gene;Acc:101175222])

HSP 1 Score: 166.392 bits (420), Expect = 2.232e-45
Identity = 115/308 (37.34%), Postives = 163/308 (52.92%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETI--NTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICS-GIPVSLLPNAVLSNSEKSKIKLAAE---NLAQTLPKID---ARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
             E++LYR+L+RANL  Y+  F+ +GGDDV QL EA +EEF EI+ LVGM  KPLH+RRLQKA+R+W  NP  FN+       PL++ P        +P  KL   E   T    +D     +++ + + +     D     +  + VS    L        +S   N    SL     G P S  P   L   + + ++   E    +A  LPK D    +      K + K +  I  M + DP RE++I +YSAIYGRF++ RR GK +  HE++VNEAAAQL ++   L+TRRD LF LARQ+ +
Sbjct:   10 GELQLYRILQRANLLYYYEAFIQQGGDDVQQLCEAGEEEFLEIMALVGMASKPLHVRRLQKALRDWVTNPAIFNQ-------PLTSLPVCS-----IPVYKLP--EGSPTLLNAQD-RANTASVKLPKAIAAACSDPGKLDVARDKVSAGSPLQGSSEVRFWSGHSNDSEHSLSPSDLGSPSS--PRDALEALDAAAVQSVLECVDRMAPGLPKTDLAEVKDQLKSNKKLAKMIGHIFEMSDDDPRREDEIRKYSAIYGRFDSKRRDGKHLTLHELTVNEAAAQLCMRDVALLTRRDELFGLARQISR 300          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Medaka
Match: nab2 (NGFI-A-binding protein 2 [Source:NCBI gene;Acc:101165801])

HSP 1 Score: 161.77 bits (408), Expect = 1.185e-43
Identity = 111/334 (33.23%), Postives = 167/334 (50.00%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEI--TNFKTEDYNCFDSNIFMSQPLLPL-------PDDENNETINTV-----------SNLMNLPSFFNSSVFSAPMNGRVKSLDIC------------SGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLPKID-ARINSTGR--KDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
             E++LYR+L+RANL  Y+  F+ +GGDDV QL EA++EEF EI+ LVGM  KPLH+RRLQKA+R+W+ NP  F++      +PL+  P  K +   +  +  K L N    +    ED  C  + +    P  P        P+    E ++ +           +  +   S       S PM      L  C            +   +S  P   L       +  + + L +TLP+ D A + +  R  K ++K +  I SM   + ++E +I +YS IYGRF++ RR GK + HHE+ +NEAAAQ  ++   L+ RR  LF LARQV +
Sbjct:    8 GELQLYRVLQRANLLAYYETFIQQGGDDVQQLCEAAEEEFLEIMALVGMATKPLHVRRLQKALRDWAANPALFSQPVAN--VPLAGIPLFKVDGMGISGEPRKSLSNGQPESPCDREDRACL-TPMHSGSPRSPCSQASPQPPETHYREKLSPLDPHWLSTEPDANGALASTSEVEEEPLSPPM------LSTCPPGPSVSPGPSFTPTGLSAWPGGQLDAETVRAVAESVDRLFRTLPRSDSAEVKTLLRMNKKMSKTVGHIFSMGPLNANKEEEIRKYSLIYGRFDSKRREGKQLTHHELIINEAAAQFCMRDNALLLRRVELFSLARQVAR 332          
BLAST of NGFI-A-binding protein 1 vs. Ensembl Medaka
Match: nab2 (NGFI-A-binding protein 2 [Source:NCBI gene;Acc:101165801])

HSP 1 Score: 161.77 bits (408), Expect = 1.185e-43
Identity = 111/334 (33.23%), Postives = 167/334 (50.00%), Query Frame = 2
Query:   56 NEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEI--TNFKTEDYNCFDSNIFMSQPLLPL-------PDDENNETINTV-----------SNLMNLPSFFNSSVFSAPMNGRVKSLDIC------------SGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLPKID-ARINSTGR--KDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVK 952
             E++LYR+L+RANL  Y+  F+ +GGDDV QL EA++EEF EI+ LVGM  KPLH+RRLQKA+R+W+ NP  F++      +PL+  P  K +   +  +  K L N    +    ED  C  + +    P  P        P+    E ++ +           +  +   S       S PM      L  C            +   +S  P   L       +  + + L +TLP+ D A + +  R  K ++K +  I SM   + ++E +I +YS IYGRF++ RR GK + HHE+ +NEAAAQ  ++   L+ RR  LF LARQV +
Sbjct:    8 GELQLYRVLQRANLLAYYETFIQQGGDDVQQLCEAAEEEFLEIMALVGMATKPLHVRRLQKALRDWAANPALFSQPVAN--VPLAGIPLFKVDGMGISGEPRKSLSNGQPESPCDREDRACL-TPMHSGSPRSPCSQASPQPPETHYREKLSPLDPHWLSTEPDANGALASTSEVEEEPLSPPM------LSTCPPGPSVSPGPSFTPTGLSAWPGGQLDAETVRAVAESVDRLFRTLPRSDSAEVKTLLRMNKKMSKTVGHIFSMGPLNANKEEEIRKYSLIYGRFDSKRREGKQLTHHELIINEAAAQFCMRDNALLLRRVELFSLARQVAR 332          
BLAST of NGFI-A-binding protein 1 vs. Planmine SMEST
Match: SMESG000013520.1 (SMESG000013520.1)

HSP 1 Score: 905.975 bits (2340), Expect = 0.000e+0
Identity = 471/472 (99.79%), Postives = 472/472 (100.00%), Query Frame = 2
Query:   29 MALTSNLCSNEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLPKIDARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVKMAEEIKNDNFKLPHNHYDDEMKHNVDCLXXXXXXXXXXXXXXHLLLQETRIRESYREAKQNNQGLLMNEIQKRLDDTLNQLNNKFTEQTTLATNRILKAGSLVXXXXXXXXXXXXEDISMMISDPGIIDNYQGTDPNSCISMNSSEEDSNYEYDIKPNIINSFL 1444
            MALTSNLCSNEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLPKIDARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVKMAEEIKNDNFKLPHNHYDDEMKHNVDCLEVEKQVVNVKNEIEHLLLQETRIRESYREAKQNNQGLLMNEIQKRLDDTLNQLNNKFTEQTTLATNRILKAGSLVNNKKFIINHNIKEDIS+MISDPGIIDNYQGTDPNSCISMNSSEEDSNYEYDIKPNIINSFL
Sbjct:    1 MALTSNLCSNEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLPKIDARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVKMAEEIKNDNFKLPHNHYDDEMKHNVDCLEVEKQVVNVKNEIEHLLLQETRIRESYREAKQNNQGLLMNEIQKRLDDTLNQLNNKFTEQTTLATNRILKAGSLVNNKKFIINHNIKEDISLMISDPGIIDNYQGTDPNSCISMNSSEEDSNYEYDIKPNIINSFL 472          
BLAST of NGFI-A-binding protein 1 vs. Planmine SMEST
Match: SMESG000013520.1 (SMESG000013520.1)

HSP 1 Score: 900.968 bits (2327), Expect = 0.000e+0
Identity = 470/473 (99.37%), Postives = 472/473 (99.79%), Query Frame = 2
Query:   29 MALTSNLCSNEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLPKIDARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVKMAEEIKNDNFKLPHNHYDDEMKHNVDCLXXXXXXXXXXXXXXHLLLQETRIRESYREAKQNNQGLLMNEIQKRLDDTLNQLNNKFTEQTTLATNR-ILKAGSLVXXXXXXXXXXXXEDISMMISDPGIIDNYQGTDPNSCISMNSSEEDSNYEYDIKPNIINSFL 1444
            MALTSNLCSNEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLPKIDARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVKMAEEIKNDNFKLPHNHYDDEMKHNVDCLEVEKQVVNVKNEIEHLLLQETRIRESYREAKQNNQGLLMNEIQKRLDDTLNQLNNKFTEQTTLATNR +LKAGSLVNNKKFIINHNIKEDIS+MISDPGIIDNYQGTDPNSCISMNSSEEDSNYEYDIKPNIINSFL
Sbjct:    1 MALTSNLCSNEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFDEIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLKANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNETINTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSEKSKIKLAAENLAQTLPKIDARINSTGRKDVNKGLMSILSMPEHDPDRENQIYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLFHLARQVVKMAEEIKNDNFKLPHNHYDDEMKHNVDCLEVEKQVVNVKNEIEHLLLQETRIRESYREAKQNNQGLLMNEIQKRLDDTLNQLNNKFTEQTTLATNRMLLKAGSLVNNKKFIINHNIKEDISLMISDPGIIDNYQGTDPNSCISMNSSEEDSNYEYDIKPNIINSFL 473          
The following BLAST results are available for this feature:
BLAST of NGFI-A-binding protein 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
NAB12.914e-4535.20NGFI-A binding protein 1 [Source:HGNC Symbol;Acc:H... [more]
NAB12.914e-4535.20NGFI-A binding protein 1 [Source:HGNC Symbol;Acc:H... [more]
NAB12.952e-4535.20NGFI-A binding protein 1 [Source:HGNC Symbol;Acc:H... [more]
NAB23.772e-4033.93NGFI-A binding protein 2 [Source:HGNC Symbol;Acc:H... [more]
NAB21.737e-3934.35NGFI-A binding protein 2 [Source:HGNC Symbol;Acc:H... [more]
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BLAST of NGFI-A-binding protein 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mab-109.494e-1941.80pep chromosome:WBcel235:II:10528084:10533880:1 gen... [more]
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BLAST of NGFI-A-binding protein 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
nab7.333e-2346.61gene:FBgn0259986 transcript:FBtr0332660[more]
nab7.606e-2346.61gene:FBgn0259986 transcript:FBtr0302032[more]
nab1.103e-2246.61gene:FBgn0259986 transcript:FBtr0302033[more]
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BLAST of NGFI-A-binding protein 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
nab1b2.187e-5038.56NGFI-A binding protein 1b (EGR1 binding protein 1)... [more]
nab1a1.002e-4434.85NGFI-A binding protein 1a (EGR1 binding protein 1)... [more]
nab21.437e-4430.98NGFI-A binding protein 2 (EGR1 binding protein 2) ... [more]
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BLAST of NGFI-A-binding protein 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
nab12.473e-4637.62NGFI-A binding protein 1 [Source:NCBI gene;Acc:100... [more]
chd37.892e-3832.51chromodomain helicase DNA binding protein 3 [Sourc... [more]
chd37.945e-3833.44chromodomain helicase DNA binding protein 3 [Sourc... [more]
chd39.965e-3832.51chromodomain helicase DNA binding protein 3 [Sourc... [more]
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BLAST of NGFI-A-binding protein 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Nab11.489e-4535.46Ngfi-A binding protein 1 [Source:MGI Symbol;Acc:MG... [more]
Nab12.198e-4536.94Ngfi-A binding protein 1 [Source:MGI Symbol;Acc:MG... [more]
Nab12.249e-4536.94Ngfi-A binding protein 1 [Source:MGI Symbol;Acc:MG... [more]
Nab25.180e-4033.95Ngfi-A binding protein 2 [Source:MGI Symbol;Acc:MG... [more]
Nab21.863e-3933.64Ngfi-A binding protein 2 [Source:MGI Symbol;Acc:MG... [more]
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BLAST of NGFI-A-binding protein 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O35589|NAB1_MESAU7.790e-4737.66NGFI-A-binding protein 1 OS=Mesocricetus auratus O... [more]
sp|Q62722|NAB1_RAT9.983e-4636.10NGFI-A-binding protein 1 OS=Rattus norvegicus OX=1... [more]
sp|Q13506|NAB1_HUMAN1.399e-4435.20NGFI-A-binding protein 1 OS=Homo sapiens OX=9606 G... [more]
sp|Q61122|NAB1_MOUSE1.537e-4436.94NGFI-A-binding protein 1 OS=Mus musculus OX=10090 ... [more]
sp|Q15742|NAB2_HUMAN8.343e-3934.35NGFI-A-binding protein 2 OS=Homo sapiens OX=9606 G... [more]
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BLAST of NGFI-A-binding protein 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2L2YVQ27.917e-5238.94NGFI-A-binding protein-like protein (Fragment) OS=... [more]
A0A1B6LVR58.262e-5240.33Uncharacterized protein (Fragment) OS=Graphocephal... [more]
A0A1A9YIH52.921e-5036.75Uncharacterized protein OS=Glossina fuscipes fusci... [more]
A0A1A9UG601.101e-4937.39Uncharacterized protein OS=Glossina austeni OX=739... [more]
A0A1A9WLK72.204e-4936.36Uncharacterized protein OS=Glossina brevipalpis OX... [more]
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BLAST of NGFI-A-binding protein 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nab1b2.871e-4938.70NGFI-A binding protein 1 [Source:NCBI gene;Acc:103... [more]
nab1b3.595e-4937.88NGFI-A binding protein 1 [Source:NCBI gene;Acc:103... [more]
nab1a1.050e-4535.48NGFI-A-binding protein 1-like [Source:NCBI gene;Ac... [more]
nab1a1.087e-4535.88NGFI-A-binding protein 1-like [Source:NCBI gene;Ac... [more]
nab1a1.087e-4535.88NGFI-A-binding protein 1-like [Source:NCBI gene;Ac... [more]
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BLAST of NGFI-A-binding protein 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000006363.12.685e-2954.17pep scaffold:Pmarinus_7.0:GL490594:2760:3775:-1 ge... [more]
ENSPMAT00000007439.14.068e-1855.56pep scaffold:Pmarinus_7.0:GL491332:428:5296:1 gene... [more]
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BLAST of NGFI-A-binding protein 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NGFI-A-binding protein 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO414079.312e-2155.38Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of NGFI-A-binding protein 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 4
Match NameE-valueIdentityDescription
nab1a1.288e-4537.34NGFI-A binding protein 1 [Source:NCBI gene;Acc:101... [more]
nab1a2.232e-4537.34NGFI-A binding protein 1 [Source:NCBI gene;Acc:101... [more]
nab21.185e-4333.23NGFI-A-binding protein 2 [Source:NCBI gene;Acc:101... [more]
nab21.185e-4333.23NGFI-A-binding protein 2 [Source:NCBI gene;Acc:101... [more]
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BLAST of NGFI-A-binding protein 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000013520.10.000e+099.79SMESG000013520.1[more]
SMESG000013520.10.000e+099.37SMESG000013520.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012203 ID=SMED30012203|Name=NGFI-A-binding protein 1|organism=Schmidtea mediterranea sexual|type=transcript|length=1481bp
CTATATAAATCTTATATGGTAATAACTTATGGCTTTAACTTCAAACCTTT
GTAGTAATGAAGTGGAATTATATCGTTTACTTGAACGTGCTAATTTAGGT
CAATATTTCACAAACTTTTTAGCAAAAGGAGGAGATGACGTTTCACAATT
GTCTGAAGCATCGAAAGAAGAATTTGATGAAATTATTGAGTTAGTTGGTA
TGAATCAAAAACCACTCCACATTAGGCGACTTCAGAAAGCAATTCGAGAG
TGGTCTAAAAATCCAGAAGAATTCAATGAATCTAAAACTGAAATCATCAT
TCCATTGTCAGCAAGACCGTATTTGAAAGCCAATCAAAATCTTGTTCCAT
GTGATAAATTAAAAAGATTAGAAAATGAAATTACTAATTTCAAAACCGAA
GATTATAATTGTTTTGATAGCAATATATTTATGTCACAGCCACTGCTTCC
ATTACCAGACGATGAAAATAATGAAACGATTAATACAGTATCCAATTTAA
TGAATTTGCCGTCATTCTTCAATTCATCAGTGTTTTCAGCACCAATGAAT
GGAAGAGTAAAATCATTAGATATATGCAGCGGAATTCCAGTTTCGCTGTT
GCCCAATGCTGTTTTATCCAATAGCGAGAAAAGTAAAATTAAACTAGCAG
CAGAAAATTTGGCTCAAACTTTACCGAAAATTGATGCCAGAATCAATTCC
ACTGGCAGAAAGGATGTTAATAAAGGTCTCATGAGTATCTTATCAATGCC
AGAACATGATCCAGACCGTGAAAATCAAATCTACCGTTACTCAGCCATTT
ATGGACGATTTAATGCCCCTAGGCGCACCGGAAAACCAATGAATCATCAC
GAAGTTTCTGTTAATGAAGCCGCTGCACAATTAAGCCTACAAATTCCAGA
ACTTATTACAAGACGGGATAATTTGTTTCATTTAGCCAGGCAGGTAGTCA
AAATGGCTGAAGAAATCAAGAATGATAACTTCAAATTGCCGCATAATCAC
TATGACGATGAAATGAAGCACAATGTCGACTGCCTAGAGGTTGAGAAACA
AGTTGTAAATGTAAAAAACGAAATTGAACACCTGCTATTGCAAGAAACTC
GGATACGCGAGAGCTACAGAGAGGCGAAACAAAACAATCAGGGTTTGCTG
ATGAATGAGATACAGAAACGCCTAGATGATACTCTCAATCAGTTAAACAA
CAAATTCACAGAACAAACTACTTTGGCCACCAATCGGATATTAAAAGCAG
GCTCCCTAGTCAATAATAAGAAGTTTATTATTAATCATAATATTAAAGAA
GATATTAGTATGATGATCTCGGATCCAGGCATTATTGACAATTATCAAGG
CACTGATCCAAATTCCTGTATTTCTATGAATTCATCGGAGGAAGATTCCA
ATTATGAATATGACATCAAGCCCAATATCATCAATTCATTTTTATAATTG
AAATAAATGTCTATTGCGATTTGAAAAAAAA
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protein sequence of SMED30012203-orf-1

>SMED30012203-orf-1 ID=SMED30012203-orf-1|Name=SMED30012203-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=473bp
MALTSNLCSNEVELYRLLERANLGQYFTNFLAKGGDDVSQLSEASKEEFD
EIIELVGMNQKPLHIRRLQKAIREWSKNPEEFNESKTEIIIPLSARPYLK
ANQNLVPCDKLKRLENEITNFKTEDYNCFDSNIFMSQPLLPLPDDENNET
INTVSNLMNLPSFFNSSVFSAPMNGRVKSLDICSGIPVSLLPNAVLSNSE
KSKIKLAAENLAQTLPKIDARINSTGRKDVNKGLMSILSMPEHDPDRENQ
IYRYSAIYGRFNAPRRTGKPMNHHEVSVNEAAAQLSLQIPELITRRDNLF
HLARQVVKMAEEIKNDNFKLPHNHYDDEMKHNVDCLEVEKQVVNVKNEIE
HLLLQETRIRESYREAKQNNQGLLMNEIQKRLDDTLNQLNNKFTEQTTLA
TNRILKAGSLVNNKKFIINHNIKEDISMMISDPGIIDNYQGTDPNSCISM
NSSEEDSNYEYDIKPNIINSFL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003712transcription cofactor activity
Vocabulary: biological process
TermDefinition
GO:0006355regulation of transcription, DNA-templated
GO:0045892negative regulation of transcription, DNA-templated
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR038398NCD2_sf
IPR039040NAB_fam
IPR006989NAB_co-repressor_dom
IPR006988Nab_N
IPR013761SAM/pointed_sf
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 375..395
NoneNo IPR availableCOILSCoilCoilcoord: 335..355
NoneNo IPR availablePRODOMPD342192coord: 1..103
e-value: 3.0E-22
score: 263.0
IPR013761Sterile alpha motif/pointed domain superfamilyGENE3DG3DSA:1.10.150.50coord: 4..87
e-value: 1.3E-8
score: 36.5
IPR013761Sterile alpha motif/pointed domain superfamilySUPERFAMILYSSF47769SAM/Pointed domaincoord: 13..76
IPR038398NAB co-repressor domain 2 superfamilyGENE3DG3DSA:3.40.390.60coord: 189..317
e-value: 8.5E-39
score: 134.4
IPR006988Nab, N-terminalPFAMPF04904NCD1coord: 10..83
e-value: 5.2E-31
score: 106.5
IPR006989NAB co-repressor, domainPFAMPF04905NCD2coord: 196..310
e-value: 2.8E-36
score: 124.2
IPR039040NAB familyPANTHERPTHR12623NGFI-A BINDING PROTEINcoord: 10..410