SMED30012160

Overview
NameSMED30012160
Smed IDSMED30012160
Length (bp)1558
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30012160 (SMED30012160) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30012160 (SMED30012160) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30012160 (SMED30012160) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30012160 (SMED30012160) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012160

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 31

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30012160h1SMcG0016190 SmedASXL_017962SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30012160h1SMcG0016190 SmedASXL_007249SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30012160h1SMcG0016190 dd_9493dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
cephalic gangliaSMED30012160h1SMcG0016190 dd_9493dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
pharynxSMED30012160h1SMcG0016190 dd_Smed_v4_9493_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30012160h1SMcG0016190 dd_Smed_v4_9493_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30012160h1SMcG0016190 dd_Smed_v4_9493_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30012160h1SMcG0016190 dd_Smed_v4_9493_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30012160h1SMcG0016190 dd_Smed_v4_9493_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30012160h1SMcG0016190 dd_Smed_v4_9493_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30012160h1SMcG0016190 dd_Smed_v4_9493_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30012160h1SMcG0016190 dd_Smed_v4_10646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30012160h1SMcG0016190 dd_Smed_v4_10646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30012160h1SMcG0016190 dd_Smed_v4_10646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30012160h1SMcG0016190 dd_Smed_v4_10646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30012160h1SMcG0016190 dd_Smed_v4_10646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30012160h1SMcG0016190 dd_Smed_v4_10646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012160h1SMcG0016190 Contig50481uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012160h1SMcG0016190 Contig50481newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012160h1SMcG0016190 Contig32589uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012160h1SMcG0016190 Contig32589newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012160h1SMcG0016190 Contig40776newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012160h1SMcG0016190 Contig40776uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012160h1SMcG0016190 Contig36839newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012160h1SMcG0016190 Contig36839uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30012160h1SMcG0016190 dd_Smed_v6_10646_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30012160h1SMcG0016190 dd_Smed_v6_9493_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30012160h1SMcG0016190 dd_Smed_v6_10646_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30012160h1SMcG0016190 dd_Smed_v6_9493_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30012160h1SMcG0016190 dd_Smed_v6_9493_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cholinergic neuronSMED30012160h1SMcG0016190 dd_Smed_v6_9493_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30012160 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.003348:925..6636 +7663
Homology
[Close]
BLAST of SMED30012160 vs. TrEMBL
Match: A0A1I8GTX7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.085e-12
Identity = 92/413 (22.28%), Postives = 166/413 (40.19%), Query Frame = 1 
Query:  157 VVKIGKQLASKLPKDQKPDSNSMNFSSHSEPEIHADIENLQRSHKNLVENANQTKSRLIEQLDNWRKYRDLVQVNDKYLTNDLTDWWRNKKYFPPQNFKDVDENIREAEEILRVLNDKQNEMKMTKSNCDSLSSENGPRNFSATAEINRKRKISS--TTP---------------------LSRLAESVETGLEKNIEEIKKYIPELENLLKEWQSFDKDTAEFEIWINEKEQMFESTIGKPSMKSTCLTDKQRTFG-EQLKKEIDGQEKHINDLLEKARVVGSRKINPLLKRIXXXXXXXXXXXXXXXXXIDAVDFDYVDKQTVG-CQNNEMQLNLSETIATQTLPEKYNISTSTEPRTRKTKVQ--------TFKQDFIVPVNANNVFGLRNSGGNRGLSKMLYPYCCTSD 1296
            VV++G++LA              N ++    EI   ++ L+ ++ NL E A   K+ L + L  W+ YRDL+Q  D++L +DL  WW  +     ++ +D +   +E   +   L+  + E+      C+       PR              S    +P                     +SRLAE V   L+ N+E +++ + +LE     W+    +  +   W+ ++E            ++  LTD    F  E+L+ E+    K ++  L +A+       + LL+R+          +  +  + +A++ +   +Q     +  E++L +S+   T   P        + P T+  K Q        T    F  PV       LRNSGG  G+S+ L+PY  T D
Sbjct:  656 VVRMGRKLA-----------EDNNGNAELRQEIDGQLKTLEENYANLKETAENIKNNLKDNLQKWKSYRDLLQSTDRWLHSDLAAWWAGRDPAVTKSAEDAERQTQETRAMQTRLSQARTEVTAAGFKCE-------PRGGVGAPVGAGAGDASGDPLSPHFSATSSAAPTAAGTTAAALSMSRLAEKVSRDLDANMERVERRLSQLETQRDAWERVSAEADDLADWLRDRE-----------AEAGALTD---IFAIERLRDEVAA--KSVSHELAEAKPQLKSARDALLRRL--------DGMAAQKRQAEALEEERRQRQMAKELEMEELRLRVSKARMTSAAPSGDGAGDGS-PGTQTAKKQQAASAMDLTDSALFDRPVRD---IELRNSGGGSGISRRLWPYHSTWD 1022
[Close]
BLAST of SMED30012160 vs. TrEMBL
Match: V3ZYT9 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_167389 PE=4 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.659e-12
Identity = 50/223 (22.42%), Postives = 113/223 (50.67%), Query Frame = 1 
Query:  250 EIHADIENLQRSHKNLVENANQTKSRLIEQLDNWRKYRDLVQVNDKYLTNDLTDWWRNKKYFPPQNFKDVDENIREAEEILRVLNDKQNEMKMTKSNCDSLSSENGPRNFSATAEINRKRKISSTTPLSRLAESVETGLEKNIEEIKKYIPELENLLKEWQSFDKDTAEFEIWINEKEQMFESTIGKPS--MKSTCLTDKQRTFGEQLKKEIDGQEKHINDLL 912
            ++ + ++NL++S+ NL   A Q K RL + L  W+ Y+ L++  ++YLT+D+ +W    +   P +  +  +     + +L  L +++ E+  T   C++L S           E   + + S  +PL++ A  V   +++ I +++K +  L +++++W+  D+  A+ + W+  +++       +P+         D  R  G  ++ E++G+   I+DLL
Sbjct: 5917 QVQSQLQNLEQSYVNLQSTAQQIKDRLQDILKKWQTYKKLLEKTEEYLTDDVVEWLEEAESNIPDSIDEAQQQREATQALLEKLYERKQELAATAHTCENLGS----------MENLNQEETSLESPLNQFAGQVNQHMQETIAKVEKRLDRLRDMMRQWEGVDRTRADLKQWLRNRQEEVADLENRPAKLHSEAAELDIDRLKG--IRDEVEGRGPAIDDLL 6127
[Close]
BLAST of SMED30012160 vs. TrEMBL
Match: A0A267F4M7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig022164g1 PE=4 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 3.905e-11
Identity = 93/417 (22.30%), Postives = 173/417 (41.49%), Query Frame = 1 
Query:  157 VVKIGKQLASKLPKDQKPDSNSMNFSSHSEPEIHADIENLQRSHKNLVENANQTKSRLIEQLDNWRKYRDLVQVNDKYLTNDLTDWWRNKKYFPPQNFKDVDENIREAEEILRVLNDKQNEMKMTKSNCD--------------SLSSENGPRNFSATAEINRKRKISSTTPL--SRLAESVETGLEKNIEEIKKYIPELENLLKEWQSFDKDTAEFEIWINEKEQMFESTIGKPSMKSTCLTDKQRTFG-EQLKKEIDGQEKHINDLLEKARVVGSRKINPLLKRIXXXXXXXXXXXXXXXXXIDAVDFDYVDKQTVG-CQNNEMQLNLSETIATQTLPEKYNISTSTEPRTRKTK------VQTFKQD-------------FIVPVNANNVFGLRNSGGNRGLSKMLYPYCCTSD 1296
            VV++G++LA              N ++    EI   ++ L+ ++ NL E A   K+ L + L  W+ YRDL+Q  D++L +DL  WW  +     ++ +D +   +E   +   L+  + E+      C+                S +    +FSAT+        ++   L  SRLAE V   L+ N+E +++ + +LE     W+    +  +   W+ ++E            ++  LTD    F  E+L+ E+    K ++  L +A+       + LL+R+          +  +  + +A++ +   +Q     +  E++L +S+   T   P        +    ++T+       QT K+              F  PV       LRNSGG  G+S+ L+PY  T D
Sbjct:  229 VVRMGRKLA-----------EDNNGNAELRQEIDGQLKTLEENYANLKETAENIKNNLKDNLQKWKSYRDLLQSTDRWLHSDLAAWWAGRDPAVTKSAEDAERQTQETRAMQTRLSQARTEVTAAGFKCEPRGGVGAPVGAGAGDASGDPLSPHFSATSSAAPTAAGTTAAALSMSRLAEKVSRDLDANMERVERRLSQLETQRDAWERVSAEADDLADWLRDRE-----------AEAGALTD---IFAIERLRDEVAA--KSVSHELAEAKPQLKSARDALLRRL--------DGMAAQKRQAEALEEERRQRQMAKELEMEELRLRVSKARMTSAAPSGDGAGDGSPHWDQQTQTPCHQGTQTAKKQQAASAMDLTDSALFDRPVRD---IELRNSGGGSGISRRLWPYHSTWD 607
[Close]
BLAST of SMED30012160 vs. TrEMBL
Match: A0A210QXB7 (Nesprin-1 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT09398 PE=4 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.848e-9
Identity = 50/242 (20.66%), Postives = 116/242 (47.93%), Query Frame = 1 
Query:  250 EIHADIENLQRSHKNLVENANQTKSRLIEQLDNWRKYRDLVQVNDKYLTNDLTDWWRNKKYFPPQNFKDVDENIREAEEILRVLNDKQNEMKMTKSNCDSLSSENGPRNFSATAEINRKRKISSTTPLSRLAESVETGLEKNIEEIKKYIPELENLLKEWQSFDKDTAEFEIWINEKEQMFESTIGKPSMKSTCLTDKQRTFGEQLKKEIDGQEKHINDLLEKARVVGSRK---INPLLKRI 966
            ++ + ++NL+ S+ NL   A Q ++RL + +  W+ Y+DL+  ND YL  +   W  +     P N +     +     +   LN  + EM      CD+L S +          +++  K    +P+S+LA+ V + L++ I +++K +  + + + +W+S D+   +   W++ K++  +    +P+       + +    + L++E+ G+   I+  L + R +       ++P+++ +
Sbjct: 5915 QVQSQMQNLEESYLNLQTTAEQIRNRLGDVMQRWQDYKDLLDKNDHYLNEEFPRWLGDCDAQVPDNLQVAQNKLDNIRTMWEKLNGLKQEMANACHRCDNLGSMDS---------LDQSDK-ELPSPMSQLADQVNSELKEAISQVEKRMENVRDTIGQWESVDRQRTDLRNWLHSKQENLQEMEQRPAKLHAEAAELEIANLQALREEVQGRGPAIDAFLNRYRELTQHNPALVDPVMRAV 6146
[Close]
BLAST of SMED30012160 vs. TrEMBL
Match: A0A267GT60 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001789g1 PE=4 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 3.017e-9
Identity = 95/411 (23.11%), Postives = 154/411 (37.47%), Query Frame = 1 
Query:  211 DSNSMNFSSHSEPEIHADIENLQRSHKNLVENANQTKSRLIEQLDNWRKYRDLVQVNDKYLTNDLTDWWRNKKYFPPQNFKDVDENIREAEEILRV---LNDKQNEMKMTKSNCDSLS-------------------------------SENGPRNFSATAEINRKRKISSTTPLSRLAESVETGLEKNIEEIKKYIPELENLLKEWQSFDKDTAEFEIWINEKEQMFESTIGKPSMKSTCLTDKQRTFG-------EQLKKEIDGQEKHINDLLEKARVVGS------RKINPLLKRIXXXXXXXXXXXXXXXXXIDAVDF-DYVDKQT-VGCQNNEMQLNLSETIATQTLPEKYNISTSTEPRTRKTKVQTFKQDFIVPVNANNVFGLRNSGGNRGLSKMLYPYCCTSD 1296
            + NS N+   SE  I   +  L  S+ NL E A Q + RL E    W+ YRD++   D+YL +DL  WW + +   P N +  DE  ++ EE   V   L   ++E+      CD  S                               S   P    ATA        S  +  SRLAE V   L+ N++ I + + EL       +  D +  +   W+ ++E    S     +++        R F          L+ + D   + ++  L + R   +      +K   L   ++ + R++        + +D VD+   +D+QT   C         S T +T  +           P              + P  +++   LRNSGG  G+SK L+PY   SD
Sbjct: 1408 EENSGNYELRSE--IEGQLSGLDESYANLKETAEQIRERLTENHQRWKSYRDVLTAADRYLHSDLVSWWSSHE---PLNVRTADEARQQTEEARAVQSRLTQLRSEIVNAALKCDCTSPSIVGNSAAAAAAASARRRYSGGSGSMFDGGSRRSPSPLLATA--------SGPSETSRLAEKVSRDLQSNLDRIDRRLAELSLRRDGLERVDTEAEDAADWLRDREAEANSLQDASALERLRDEVANRAFSPGLLEARPDLRAQRDALLRKLDSALSQRRQSQAVEAEYRKKQAALDSEVDSVMRRVDNRTAASAQTLDQVDYMRLMDQQTQTVCHQQTQTPKQSNTPSTSAV--DLTDGRLYGPGHGYPGHHHHHHHHLAPTGSSS--DLRNSGGGVGISKKLWPYTRESD 1801
[Close]
BLAST of SMED30012160 vs. Planmine SMEST
Match: SMESG000069851.1 (SMESG000069851.1)

HSP 1 Score: 889.797 bits (2298), Expect = 0.000e+0
Identity = 444/444 (100.00%), Postives = 444/444 (100.00%), Query Frame = 1 
Query:  157 VVKIGKQLASKLPKDQKPDSNSMNFSSHSEPEIHADIENLQRSHKNLVENANQTKSRLIEQLDNWRKYRDLVQVNDKYLTNDLTDWWRNKKYFPPQNFKDVDENIREAEEILRVLNDKQNEMKMTKSNCDSLSSENGPRNFSATAEINRKRKISSTTPLSRLAESVETGLEKNIEEIKKYIPELENLLKEWQSFDKDTAEFEIWINEKEQMFESTIGKPSMKSTCLTDKQRTFGEQLKKEIDGQEKHINDLLEKARVVGSRKINPLLKRIXXXXXXXXXXXXXXXXXIDAVDFDYVDKQTVGCQNNEMQLNLSETIATQTLPEKYNISTSTEPRTRKTKVQTFKQDFIVPVNANNVFGLRNSGGNRGLSKMLYPYCCTSDLLNQKLHLDYVHGSRLKNNQKSFIPNAPTRPIDGGKYPPRLGISKVRSAAEESVRRNWMTQDSI 1488
            VVKIGKQLASKLPKDQKPDSNSMNFSSHSEPEIHADIENLQRSHKNLVENANQTKSRLIEQLDNWRKYRDLVQVNDKYLTNDLTDWWRNKKYFPPQNFKDVDENIREAEEILRVLNDKQNEMKMTKSNCDSLSSENGPRNFSATAEINRKRKISSTTPLSRLAESVETGLEKNIEEIKKYIPELENLLKEWQSFDKDTAEFEIWINEKEQMFESTIGKPSMKSTCLTDKQRTFGEQLKKEIDGQEKHINDLLEKARVVGSRKINPLLKRIEDLKRQLKKLLERKDEEIDAVDFDYVDKQTVGCQNNEMQLNLSETIATQTLPEKYNISTSTEPRTRKTKVQTFKQDFIVPVNANNVFGLRNSGGNRGLSKMLYPYCCTSDLLNQKLHLDYVHGSRLKNNQKSFIPNAPTRPIDGGKYPPRLGISKVRSAAEESVRRNWMTQDSI
Sbjct: 1463 VVKIGKQLASKLPKDQKPDSNSMNFSSHSEPEIHADIENLQRSHKNLVENANQTKSRLIEQLDNWRKYRDLVQVNDKYLTNDLTDWWRNKKYFPPQNFKDVDENIREAEEILRVLNDKQNEMKMTKSNCDSLSSENGPRNFSATAEINRKRKISSTTPLSRLAESVETGLEKNIEEIKKYIPELENLLKEWQSFDKDTAEFEIWINEKEQMFESTIGKPSMKSTCLTDKQRTFGEQLKKEIDGQEKHINDLLEKARVVGSRKINPLLKRIEDLKRQLKKLLERKDEEIDAVDFDYVDKQTVGCQNNEMQLNLSETIATQTLPEKYNISTSTEPRTRKTKVQTFKQDFIVPVNANNVFGLRNSGGNRGLSKMLYPYCCTSDLLNQKLHLDYVHGSRLKNNQKSFIPNAPTRPIDGGKYPPRLGISKVRSAAEESVRRNWMTQDSI 1906
The following BLAST results are available for this feature:
BLAST of SMED30012160 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012160 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012160 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012160 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012160 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012160 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012160 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012160 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8GTX71.085e-1222.28Uncharacterized protein OS=Macrostomum lignano OX=... [more]
V3ZYT91.659e-1222.42Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
A0A267F4M73.905e-1122.30Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A210QXB71.848e-920.66Nesprin-1 OS=Mizuhopecten yessoensis OX=6573 GN=KP... [more]
A0A267GT603.017e-923.11Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of SMED30012160 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012160 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012160 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012160 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012160 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012160 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000069851.10.000e+0100.00SMESG000069851.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012160 ID=SMED30012160|Name=SMED30012160|organism=Schmidtea mediterranea sexual|type=transcript|length=1558bp
GAGGGATTCCCGAAACTTACGCGATCGCTTACCACCCAGACTAGGTGGCA
ACGCTTTTTAAAATACTACCATCTCAAACGTCAAATAACTGTATGACAAT
ACGGTTTTCATAGAAAACTGCTGCCTATTTCAAATCTCAAACATTAAGAC
TTCTAGGTTGTTAAAATTGGAAAGCAGTTGGCCAGTAAACTTCCAAAGGA
TCAAAAACCTGATAGTAATTCAATGAATTTTAGCTCTCATTCTGAACCAG
AAATCCATGCTGATATCGAAAACTTACAGAGAAGTCACAAGAATTTAGTA
GAGAATGCCAACCAAACAAAAAGTCGATTGATAGAACAATTGGACAACTG
GAGAAAATACAGAGATCTGGTACAGGTCAATGACAAATACCTGACCAATG
ATCTGACTGATTGGTGGAGAAATAAAAAATATTTTCCCCCTCAAAACTTT
AAAGATGTAGATGAAAACATTAGAGAAGCGGAAGAAATACTTCGCGTGCT
AAATGACAAGCAAAATGAAATGAAAATGACAAAATCAAATTGTGATTCAT
TATCATCAGAAAATGGACCGAGAAATTTTTCAGCAACCGCTGAAATCAAC
AGGAAAAGGAAAATATCATCTACAACTCCTCTTTCAAGATTAGCTGAAAG
TGTAGAAACAGGCCTCGAAAAAAATATCGAAGAAATAAAAAAATATATTC
CTGAATTAGAGAATTTGTTAAAAGAATGGCAAAGTTTTGACAAAGACACA
GCAGAATTTGAAATCTGGATAAATGAAAAAGAGCAAATGTTTGAAAGTAC
AATTGGAAAACCAAGCATGAAGAGCACATGCTTGACTGACAAGCAAAGAA
CATTTGGTGAGCAATTGAAAAAAGAAATCGACGGTCAAGAAAAACACATC
AACGATTTATTAGAAAAAGCTCGAGTGGTGGGCAGTAGGAAAATAAACCC
TTTGCTTAAAAGAATCGAAGACCTTAAACGACAATTAAAAAAGTTATTAG
AAAGAAAAGATGAAGAAATCGATGCAGTTGATTTTGATTATGTTGATAAA
CAAACCGTTGGTTGTCAGAATAATGAAATGCAGCTAAATTTGTCAGAAAC
AATTGCGACTCAAACTCTCCCGGAAAAATACAACATCAGCACATCTACAG
AACCAAGAACAAGAAAGACGAAGGTCCAGACATTTAAACAAGATTTTATA
GTTCCTGTCAACGCAAACAATGTTTTTGGGTTGAGAAACAGTGGTGGAAA
TAGAGGGCTCTCCAAGATGTTGTACCCGTATTGCTGTACGTCTGATCTGC
TCAACCAGAAACTACATTTAGATTATGTTCACGGTTCCAGATTAAAAAAC
AATCAAAAATCATTTATTCCAAATGCCCCCACAAGACCAATTGATGGGGG
GAAATATCCTCCTAGATTGGGAATTTCCAAAGTTAGAAGTGCAGCTGAAG
AATCCGTCCGACGAAACTGGATGACTCAAGATTCTATATAATGAATACAT
TATTAGAATTTTTTCTATATTTATGCTTATTATTTTTAATAGAATAAATA
TTCTTAAG
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protein sequence of SMED30012160-orf-1

>SMED30012160-orf-1 ID=SMED30012160-orf-1|Name=SMED30012160-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=423bp
MNFSSHSEPEIHADIENLQRSHKNLVENANQTKSRLIEQLDNWRKYRDLV
QVNDKYLTNDLTDWWRNKKYFPPQNFKDVDENIREAEEILRVLNDKQNEM
KMTKSNCDSLSSENGPRNFSATAEINRKRKISSTTPLSRLAESVETGLEK
NIEEIKKYIPELENLLKEWQSFDKDTAEFEIWINEKEQMFESTIGKPSMK
STCLTDKQRTFGEQLKKEIDGQEKHINDLLEKARVVGSRKINPLLKRIED
LKRQLKKLLERKDEEIDAVDFDYVDKQTVGCQNNEMQLNLSETIATQTLP
EKYNISTSTEPRTRKTKVQTFKQDFIVPVNANNVFGLRNSGGNRGLSKML
YPYCCTSDLLNQKLHLDYVHGSRLKNNQKSFIPNAPTRPIDGGKYPPRLG
ISKVRSAAEESVRRNWMTQDSI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000044cephalic ganglia
PLANA:0000099neuron
PLANA:0000418head
PLANA:0000420parapharyngeal region
PLANA:0000463cholinergic neuron