radial spoke head protein 9 homolog

Overview
Nameradial spoke head protein 9 homolog
Smed IDSMED30012136
Length (bp)947
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of radial spoke head protein 9 homolog (SMED30012136) t-SNE clustered cells

Violin plots show distribution of expression levels for radial spoke head protein 9 homolog (SMED30012136) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of radial spoke head protein 9 homolog (SMED30012136) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for radial spoke head protein 9 homolog (SMED30012136) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012136

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 14

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30012136SMESG000046857.1 SmedASXL_008633SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30012136SMESG000046857.1 dd_Smed_v4_4707_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30012136SMESG000046857.1 dd_Smed_v4_4707_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30012136SMESG000046857.1 dd_Smed_v4_4707_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30012136SMESG000046857.1 dd_Smed_v4_4707_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30012136SMESG000046857.1 dd_Smed_v4_4707_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30012136SMESG000046857.1 dd_Smed_v4_4707_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
neoblastSMED30012136SMESG000046857.1 dd_Smed_v4_4707_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30012136SMESG000046857.1 dd_Smed_v4_4707_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30012136SMESG000046857.1 dd_Smed_v4_4707_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012136SMESG000046857.1 Contig47102newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012136SMESG000046857.1 Contig47102uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30012136SMESG000046857.1 dd_Smed_v6_4707_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30012136SMESG000046857.1 dd_Smed_v4_4707_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of radial spoke head protein 9 homolog vs. Ensembl Human
Match: RSPH9 (radial spoke head component 9 [Source:HGNC Symbol;Acc:HGNC:21057])

HSP 1 Score: 289.271 bits (739), Expect = 4.233e-97
Identity = 129/257 (50.19%), Postives = 193/257 (75.10%), Query Frame = 1 
Query:  133 LSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPSVEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            LSP+++A L +SL+++K + ++++V+FWG I G  +DY++ QG+  D++  +KTLYS +C +W +LPP + E+  +++  KGRF GDPS E+EH E+++V  EG++V EE+  V IKEE RL +VI +ID  V I+PR A  K P G    NR+FEGLS+SE+ K SSYFHFREPVEL+  + ++ A LD ++DF+DS+E D+P+ SW++Q +RG++LV+L+SLLWPG  FYH P ++N+G +Y+G GE N+DLPFM
Sbjct:   20 LSPDRRASLLTSLMLVKRDYRYDRVLFWGRILGLVADYYIAQGLSEDQLAPRKTLYSLNCTEWSLLPPATEEMVAQSSVVKGRFMGDPSYEYEHTELQKV-NEGEKVFEEEIVVQIKEETRLVSVIDQIDKAVAIIPRGALFKTPFGPTHVNRTFEGLSLSEAKKLSSYFHFREPVELKNKTLLEKADLDPSLDFMDSLEHDIPKGSWSIQMERGNALVVLRSLLWPGLTFYHAPRTKNYGYVYVGTGEKNMDLPFM 275
[Close]
BLAST of radial spoke head protein 9 homolog vs. Ensembl Human
Match: RSPH9 (radial spoke head component 9 [Source:HGNC Symbol;Acc:HGNC:21057])

HSP 1 Score: 188.348 bits (477), Expect = 2.701e-57
Identity = 95/229 (41.48%), Postives = 145/229 (63.32%), Query Frame = 1 
Query:  124 GILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPSVEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLW 810
            G  LSP+++A L +SL+++K + ++++V+FWG I G  +DY++ QG+  D++  +KTLYS +C +W +LPP + E+  +++  KGRF GDPS E+EH E+++V  EG++V EE+                 ID  V I+PR A  K P G    NR+FEGLS+SE+ K SSYFHFREPVEL+  + ++ A LD ++DF+DS+E D+P         +GS  V+     W
Sbjct:   17 GQGLSPDRRASLLTSLMLVKRDYRYDRVLFWGRILGLVADYYIAQGLSEDQLAPRKTLYSLNCTEWSLLPPATEEMVAQSSVVKGRFMGDPSYEYEHTELQKV-NEGEKVFEEEIV---------------IDKAVAIIPRGALFKTPFGPTHVNRTFEGLSLSEAKKLSSYFHFREPVELKNKTLLEKADLDPSLDFMDSLEHDIP---------KGSEAVVQGDFTW 220
[Close]
BLAST of radial spoke head protein 9 homolog vs. Ensembl Fly
Match: CG31803 (gene:FBgn0051803 transcript:FBtr0081010)

HSP 1 Score: 165.236 bits (417), Expect = 6.870e-49
Identity = 99/274 (36.13%), Postives = 150/274 (54.74%), Query Frame = 1 
Query:  103 IDYLGSSGILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILP-PPSVEIREKAAFFKGRFSGDPSNEFEHIEVKQVPG---EGDEVH--EEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEM-SWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            +D L   G+ LSPEQ+ ++++SL+ L+N+ +F  +  WG I  TK+DY++  G   D + D+K  YS D  +W +LP   S +I +     +  F GDPS       VK  P     G++V      E V +KEE+RLAA++  I  +  I PR A+ K+  G+++ N+ F GL+  +   +S Y  +R P    K +    +  + AIDFLD+I+  +P   ++AL   R   LVI+KS LW G  F+H   S   G LY+G G+ N DL FM
Sbjct:   10 LDCLMYCGMKLSPEQRILIENSLIALQNDNRFTGMYLWGRITATKNDYYIAFGYTNDCLKDRKYFYSLDQFQWQLLPFVQSPKIFQATILAREPFIGDPS---LMTYVKLDPTFDIVGNQVAGISRPEVVKLKEEERLAAIVFIITEECAICPRGAFYKMTDGRVIPNQMFRGLNDLQIDNQSYYQLYRLPRNDLKVNLAKRSDYNYAIDFLDTIDCVIPLCQAFALNMQRNEGLVIIKSCLWLGMTFFHKINSHKHGFLYLGDGKKNFDLLFM 280
[Close]
BLAST of radial spoke head protein 9 homolog vs. Ensembl Zebrafish
Match: rsph9 (radial spoke head component 9 [Source:NCBI gene;Acc:558005])

HSP 1 Score: 295.819 bits (756), Expect = 8.527e-100
Identity = 135/277 (48.74%), Postives = 197/277 (71.12%), Query Frame = 1 
Query:   76 MNSSDLHLMIDYLGSSGILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPSVE-IREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            M+S  LH  +D   ++G+ LS EQ+A LQSSL+I+K   KF++V+FWG I G KSDYF+ QG+  DE+ ++K+LYS +C+ W +LPP +   I + A    GRF+GDPS+E+EH E++   GEGDE   E+ TV + E  RLAA+++ ID DV +VPR A+IK P G++ +NRSF GL  +E+AK  +Y HFREPV L+  S ++++ L+ A+DFLD +  D+ + SW+LQ DRG ++ +L+SLLW G+ F+HVP++   G +YMG G +N+DLPFM
Sbjct:    1 MDSDSLHYSLDLAAANGLTLSSEQRAALQSSLLIVKRNYKFSRVLFWGKILGIKSDYFIAQGVEDDELKNRKSLYSLNCVDWHLLPPATESMIADVALAATGRFTGDPSHEYEHTEIR-TEGEGDEATHEEVTVKVIEASRLAAIVSNIDKDVSVVPRGAFIKSPNGKVQTNRSFGGLHPTEAAKLRNYLHFREPVNLRNKSILEMSELNPALDFLDPLSEDILKGSWSLQLDRGGTVCVLRSLLWLGFTFFHVPQTPQHGYIYMGDGLMNLDLPFM 276
[Close]
BLAST of radial spoke head protein 9 homolog vs. Ensembl Xenopus
Match: rsph9 (radial spoke head 9 homolog [Source:NCBI gene;Acc:448532])

HSP 1 Score: 161.384 bits (407), Expect = 7.538e-49
Identity = 77/142 (54.23%), Postives = 98/142 (69.01%), Query Frame = 1 
Query:  478 IKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            IKEE RL   IA ID +  + PR AYI+ P GQ++ N SF GL VSE  K SSYFHF   +  +K S ++ A LD +IDFLDS+E D+P  SW+LQ ++G S++IL+SLLW G  FYHVP +   G LY+G GE N+DLPFM
Sbjct:    3 IKEEVRLTGTIAMIDREAAVAPRGAYIRNPLGQVIVNHSFRGLEVSEGKKLSSYFHFTPSLNPKKKSLLEKAALDPSIDFLDSLEHDIPRGSWSLQLEQGDSVLILRSLLWLGMTFYHVPLTPLHGHLYIGTGERNLDLPFM 144
[Close]
BLAST of radial spoke head protein 9 homolog vs. Ensembl Mouse
Match: Rsph9 (radial spoke head 9 homolog (Chlamydomonas) [Source:MGI Symbol;Acc:MGI:1922814])

HSP 1 Score: 275.018 bits (702), Expect = 9.761e-92
Identity = 122/257 (47.47%), Postives = 187/257 (72.76%), Query Frame = 1 
Query:  133 LSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPSVEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            LSP+++A L +SL+++K + +F +V+FWG I G  +DY++ QG+  D++  +KTLYS +C +W +LPP + E+  + +   GRF GDPS+E+EH E+++V  EG++V +E+  V IKEE RL ++I +ID  V I+PR A  K P G    NR+FEGL +SE  K SSYFHFRE ++L+  + ++ + L+ ++DFLDS+E D+P  SW++Q +RG++LV+L+SLLWPG  FYH P ++N+G +Y+G GE N+DLPFM
Sbjct:   20 LSPDRRASLLTSLMLVKRDYRFARVLFWGRILGLVADYYIAQGLSEDQLAPRKTLYSLNCTEWSLLPPATEEMAMQISVVSGRFMGDPSHEYEHTELQKV-NEGEKVFDEEVVVQIKEETRLVSIIDQIDKAVAIIPRGALFKTPFGVTHVNRTFEGLPLSEVRKLSSYFHFREAIDLKNKTLLEKSDLEPSLDFLDSLEYDIPRGSWSIQMERGNALVVLRSLLWPGLTFYHAPRTKNYGYIYVGTGEKNMDLPFM 275
[Close]
BLAST of radial spoke head protein 9 homolog vs. Ensembl Mouse
Match: Rsph9 (radial spoke head 9 homolog (Chlamydomonas) [Source:MGI Symbol;Acc:MGI:1922814])

HSP 1 Score: 79.337 bits (194), Expect = 1.987e-18
Identity = 37/79 (46.84%), Postives = 54/79 (68.35%), Query Frame = 1 
Query:  514 EIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEM 750
            +ID  V I+PR A  K P G    NR+FEGL +SE  K SSYFHFRE ++L+  + ++ + L+ ++DFLDS+E D+P +
Sbjct:    2 QIDKAVAIIPRGALFKTPFGVTHVNRTFEGLPLSEVRKLSSYFHFREAIDLKNKTLLEKSDLEPSLDFLDSLEYDIPRV 80
[Close]
BLAST of radial spoke head protein 9 homolog vs. UniProt/SwissProt
Match: sp|Q5TYW6|RSPH9_DANRE (Radial spoke head protein 9 homolog OS=Danio rerio OX=7955 GN=rsph9 PE=2 SV=1)

HSP 1 Score: 295.819 bits (756), Expect = 6.437e-99
Identity = 135/277 (48.74%), Postives = 197/277 (71.12%), Query Frame = 1 
Query:   76 MNSSDLHLMIDYLGSSGILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPSVE-IREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            M+S  LH  +D   ++G+ LS EQ+A LQSSL+I+K   KF++V+FWG I G KSDYF+ QG+  DE+ ++K+LYS +C+ W +LPP +   I + A    GRF+GDPS+E+EH E++   GEGDE   E+ TV + E  RLAA+++ ID DV +VPR A+IK P G++ +NRSF GL  +E+AK  +Y HFREPV L+  S ++++ L+ A+DFLD +  D+ + SW+LQ DRG ++ +L+SLLW G+ F+HVP++   G +YMG G +N+DLPFM
Sbjct:    1 MDSDSLHYSLDLAAANGLTLSSEQRAALQSSLLIVKRNYKFSRVLFWGKILGIKSDYFIAQGVEDDELKNRKSLYSLNCVDWHLLPPATESMIADVALAATGRFTGDPSHEYEHTEIR-TEGEGDEATHEEVTVKVIEASRLAAIVSNIDKDVSVVPRGAFIKSPNGKVQTNRSFGGLHPTEAAKLRNYLHFREPVNLRNKSILEMSELNPALDFLDPLSEDILKGSWSLQLDRGGTVCVLRSLLWLGFTFFHVPQTPQHGYIYMGDGLMNLDLPFM 276
[Close]
BLAST of radial spoke head protein 9 homolog vs. UniProt/SwissProt
Match: sp|Q9H1X1|RSPH9_HUMAN (Radial spoke head protein 9 homolog OS=Homo sapiens OX=9606 GN=RSPH9 PE=1 SV=1)

HSP 1 Score: 289.271 bits (739), Expect = 2.033e-96
Identity = 129/257 (50.19%), Postives = 193/257 (75.10%), Query Frame = 1 
Query:  133 LSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPSVEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            LSP+++A L +SL+++K + ++++V+FWG I G  +DY++ QG+  D++  +KTLYS +C +W +LPP + E+  +++  KGRF GDPS E+EH E+++V  EG++V EE+  V IKEE RL +VI +ID  V I+PR A  K P G    NR+FEGLS+SE+ K SSYFHFREPVEL+  + ++ A LD ++DF+DS+E D+P+ SW++Q +RG++LV+L+SLLWPG  FYH P ++N+G +Y+G GE N+DLPFM
Sbjct:   20 LSPDRRASLLTSLMLVKRDYRYDRVLFWGRILGLVADYYIAQGLSEDQLAPRKTLYSLNCTEWSLLPPATEEMVAQSSVVKGRFMGDPSYEYEHTELQKV-NEGEKVFEEEIVVQIKEETRLVSVIDQIDKAVAIIPRGALFKTPFGPTHVNRTFEGLSLSEAKKLSSYFHFREPVELKNKTLLEKADLDPSLDFMDSLEHDIPKGSWSIQMERGNALVVLRSLLWPGLTFYHAPRTKNYGYVYVGTGEKNMDLPFM 275
[Close]
BLAST of radial spoke head protein 9 homolog vs. UniProt/SwissProt
Match: sp|Q2KIU7|RSPH9_BOVIN (Radial spoke head protein 9 homolog OS=Bos taurus OX=9913 GN=RSPH9 PE=2 SV=1)

HSP 1 Score: 282.337 bits (721), Expect = 1.154e-93
Identity = 132/261 (50.57%), Postives = 197/261 (75.48%), Query Frame = 1 
Query:  121 SGILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPSVEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            SG  LSP+++A L +SL+++K + +F++V+FWG I G  +DY++ QG+  D++  +KTLYS +C++W +LPP + E+  + +  KGRF GDPS+E+EH E+++V  EG++V EE+  V IKEE RL +VI +ID  V ++PR A  K P G I  NR+FEGLS+SE+ K SSYFHFREPVEL+  + ++ A LD ++DF+DS+E D+P+ SW++Q +RG++LV+L+SLLWPG  FYH P ++N+G +Y+G GE N+DLPFM
Sbjct:   16 SGQGLSPDRRASLLTSLMLVKRDYRFDRVLFWGRILGLVADYYIAQGVSEDQLAPRKTLYSLNCMEWSLLPPATKEMETQTSVVKGRFMGDPSHEYEHTELQKV-NEGEKVFEEEVVVQIKEETRLVSVIDQIDKAVAVIPRGALFKTPFGPINVNRTFEGLSLSEAKKLSSYFHFREPVELKNKTLLEKAELDPSLDFMDSLEHDIPKGSWSVQMERGNALVVLRSLLWPGLTFYHAPRTKNYGYIYVGTGEKNLDLPFM 275
[Close]
BLAST of radial spoke head protein 9 homolog vs. UniProt/SwissProt
Match: sp|Q9D9V4|RSPH9_MOUSE (Radial spoke head protein 9 homolog OS=Mus musculus OX=10090 GN=Rsph9 PE=1 SV=1)

HSP 1 Score: 275.018 bits (702), Expect = 6.827e-91
Identity = 122/257 (47.47%), Postives = 187/257 (72.76%), Query Frame = 1 
Query:  133 LSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPSVEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            LSP+++A L +SL+++K + +F +V+FWG I G  +DY++ QG+  D++  +KTLYS +C +W +LPP + E+  + +   GRF GDPS+E+EH E+++V  EG++V +E+  V IKEE RL ++I +ID  V I+PR A  K P G    NR+FEGL +SE  K SSYFHFRE ++L+  + ++ + L+ ++DFLDS+E D+P  SW++Q +RG++LV+L+SLLWPG  FYH P ++N+G +Y+G GE N+DLPFM
Sbjct:   20 LSPDRRASLLTSLMLVKRDYRFARVLFWGRILGLVADYYIAQGLSEDQLAPRKTLYSLNCTEWSLLPPATEEMAMQISVVSGRFMGDPSHEYEHTELQKV-NEGEKVFDEEVVVQIKEETRLVSIIDQIDKAVAIIPRGALFKTPFGVTHVNRTFEGLPLSEVRKLSSYFHFREAIDLKNKTLLEKSDLEPSLDFLDSLEYDIPRGSWSIQMERGNALVVLRSLLWPGLTFYHAPRTKNYGYIYVGTGEKNMDLPFM 275
[Close]
BLAST of radial spoke head protein 9 homolog vs. UniProt/SwissProt
Match: sp|Q6DFN5|RSPH9_XENTR (Radial spoke head protein 9 homolog OS=Xenopus tropicalis OX=8364 GN=rsph9 PE=2 SV=1)

HSP 1 Score: 238.039 bits (606), Expect = 3.130e-76
Identity = 123/277 (44.40%), Postives = 174/277 (62.82%), Query Frame = 1 
Query:   76 MNSSDLHLMIDYLGSSGILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGA-DEMTDKKTLYSKDCLKWVILPPPSVEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            M++  L L ++ +  SG  LS EQ A L++SL +L  + +  ++  WG + G + DY + +G G  D +  + + YS +C+ W +LPP +     +    KGRF GDP++E+E+I V+   G GD   EE+ T  IKEE RL   IA ID +  + PR AYI+ P GQ++ N SF GL VSE  K SSYFHF   +  +K S ++ A LD +IDFLDS+E D+P  SW+LQ ++G S++IL+SLLW G  FYHVP +   G LY+G GE N+DLPFM
Sbjct:    1 MDAETLSLSLELVSGSGPGLSAEQCAALRASLPLLHRDLRLRRLRLWGVVLGLRGDYVIARGWGGPDLLRGQLSFYSLNCVDWCLLPPATDAHIAQTQGIKGRFVGDPAHEYEYI-VRNTAGGGDSALEEELTTHIKEEVRLTGTIAMIDREAAVAPRGAYIRNPLGQVIVNHSFRGLEVSEGKKLSSYFHFTPSLNPKKKSLLEKAALDPSIDFLDSLEHDIPRGSWSLQLEQGDSVLILRSLLWLGMTFYHVPLTPLHGHLYIGTGERNLDLPFM 276
[Close]
BLAST of radial spoke head protein 9 homolog vs. TrEMBL
Match: A0A1S3KIZ6 (radial spoke head protein 9 homolog OS=Lingula unguis OX=7574 GN=LOC106182087 PE=4 SV=1)

HSP 1 Score: 368.237 bits (944), Expect = 4.497e-125
Identity = 179/276 (64.86%), Postives = 218/276 (78.99%), Query Frame = 1 
Query:   76 MNSSDLHLMIDYLGSSGILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPSVEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            M +  LHL +DY+GSSG +LSPEQKA LQ+SLVILKN QKFN+V +WG I G K DYF+ QGIG DEM DK TLYSKDC+ W +LPP +  +++ +   KGRF+GDPS EFEH+EVK+  GE DEV EE+ET+ IKEEDRLA+VI++IDNDV IVPRAA+IK P GQ+V NRSFEGL+V E+AK SSY HFR+ V+L + + +  A LDKAIDFLD IE D+P+ SW+LQF+RGS LV L+SLLW GYVFYHVP +R FG +Y G GE N DLPFM
Sbjct:    1 MEAESLHLEVDYVGSSGTILSPEQKAALQTSLVILKNNQKFNRVYYWGKILGVKDDYFIAQGIGKDEMNDKTTLYSKDCVNWNLLPPATDAMKKTSRLAKGRFTGDPSYEFEHVEVKKTYGENDEVQEEEETIPIKEEDRLASVISDIDNDVSIVPRAAFIKRPAGQVVKNRSFEGLTVQEAAKLSSYMHFRDAVKLNEKTLLQRANLDKAIDFLDIIEEDIPKGSWSLQFERGSGLVTLRSLLWLGYVFYHVPGTRKFGCVYTGTGEKNQDLPFM 276
[Close]
BLAST of radial spoke head protein 9 homolog vs. TrEMBL
Match: A0A504YY28 (Radial spoke head protein 9 OS=Fasciola gigantica OX=46835 GN=FGIG_10030 PE=4 SV=1)

HSP 1 Score: 367.081 bits (941), Expect = 1.243e-124
Identity = 164/276 (59.42%), Postives = 216/276 (78.26%), Query Frame = 1 
Query:   76 MNSSDLHLMIDYLGSSGILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPSVEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            M++ +LHL ID++GS G++L+PEQKA LQ+SL ILK+E  F  VVFWG ++G K DYF+ QGIG D MT +K LYSKDCL W ILP P     EK+ +FKGR +GDPS E E++++KQ+PGEGDE+ E +E +T+KEEDRLAA++  ID++V IVPR AY++LP+GQ+V N+SFEGL+V+E++K  SYFHFR P+ L     +D ARLDKAIDFLD+IE D P+  W +QF+RGS LV++KSLLW GYV YH+P +  +GS+Y+G GE NIDLPFM
Sbjct:    1 MDAENLHLNIDFVGSCGVVLTPEQKATLQTSLTILKHENNFTNVVFWGIVRGVKGDYFIAQGIGKDAMTSRKNLYSKDCLVWGILPVPGKSDIEKSKYFKGRLTGDPSYEAEYVDIKQMPGEGDELVEREELITMKEEDRLAAIVERIDDEVMIVPRGAYMRLPSGQVVRNKSFEGLTVAEASKALSYFHFRPPIHLPHKPLIDRARLDKAIDFLDTIEDDNPKGCWTIQFERGSRLVLIKSLLWIGYVLYHLPGTNKYGSIYVGTGEYNIDLPFM 276
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BLAST of radial spoke head protein 9 homolog vs. TrEMBL
Match: Q5D8R2 (Radial spoke head protein 9 isoform 2 (Fragment) OS=Schistosoma japonicum OX=6182 GN=EWB00_004410 PE=2 SV=1)

HSP 1 Score: 351.673 bits (901), Expect = 1.434e-118
Identity = 161/276 (58.33%), Postives = 213/276 (77.17%), Query Frame = 1 
Query:   76 MNSSDLHLMIDYLGSSGILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPSVEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            M+SSDLHL IDY+GS G +L+PEQKA LQ++L ILK+E KF+ V+FWG I+    DYF+ QGIG D + +K  LYSKDC  W +LP P  +  EK+  F+ R +GDPS E EHIEVKQ+PGEGDE+ E +E +T+KEEDRLAA+I  I+ DV IVPR AY+++P+GQ+V N+SFEGL+++E++K+ SYFH R P ++     ++ A+LDKAIDFLD+IE D PE  W +QF+RGS+LV++KSLLW GYV YHVP +  +GS+Y+G GE NIDLPFM
Sbjct:    1 MDSSDLHLSIDYVGSCGFILTPEQKATLQTTLTILKHENKFSYVLFWGIIRAVNGDYFIAQGIGKDVLKEKTNLYSKDCCTWGLLPVPGKQDIEKSKVFRTRLTGDPSYEAEHIEVKQLPGEGDELAETEELITMKEEDRLAAIIYRIEEDVVIVPRGAYLRMPSGQVVRNKSFEGLTIAEASKQLSYFHCRPP-KIINMPLVERAKLDKAIDFLDTIEDDNPEGCWVIQFERGSTLVLIKSLLWIGYVLYHVPGTSKYGSIYVGTGEYNIDLPFM 275
[Close]
BLAST of radial spoke head protein 9 homolog vs. TrEMBL
Match: A0A0B7ABU3 (Uncharacterized protein OS=Arion vulgaris OX=1028688 GN=ORF110341 PE=4 SV=1)

HSP 1 Score: 350.903 bits (899), Expect = 3.439e-118
Identity = 163/276 (59.06%), Postives = 209/276 (75.72%), Query Frame = 1 
Query:   76 MNSSDLHLMIDYLGSSGILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPSVEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            M+S   HL ID +GSSGILLSPEQKA LQ SLVIL+NE KF+KV FWG I G K+DYFV QG+  DE   +K  YSKDC+ W +LPP + +++  A   KGRF+GDPS E EH+E K+V GEGD+  EE+E   IKEEDRLAAVIA+I+ DV+IVPR A+I+ PT ++V+NRSFEGL+V E++K  +Y HFR+   L   + +  A +D+AIDF+DSIE D+P  SW++QF+RGS L+ L+SLLWPGY FYHVP +R +GS+Y G GE N+DLPFM
Sbjct:    1 MDSRSFHLSIDCVGSSGILLSPEQKAALQISLVILQNENKFSKVYFWGKIVGVKNDYFVTQGVYRDEFAGRKIFYSKDCIHWGLLPPATAQMKANAKLEKGRFTGDPSFEVEHVEFKKV-GEGDDATEEEEVTVIKEEDRLAAVIADINADVWIVPRGAFIQAPTAEVVNNRSFEGLTVQEASKLCNYMHFRKAKTLDFKNLLQRANVDRAIDFMDSIEDDIPNGSWSIQFERGSGLLTLRSLLWPGYSFYHVPATREYGSVYFGTGERNLDLPFM 275
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BLAST of radial spoke head protein 9 homolog vs. TrEMBL
Match: A0A210QAA3 (Radial spoke head protein 9-like OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT10148 PE=4 SV=1)

HSP 1 Score: 348.977 bits (894), Expect = 5.428e-117
Identity = 165/276 (59.78%), Postives = 220/276 (79.71%), Query Frame = 1 
Query:   76 MNSSDLHLMIDYLGSSGILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPSVEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            M SS LHL IDY+GSSG++LSPEQKA LQ+SLVIL++  KF+KV  WG I G K DYF+ QG+  DEM +KKT YSKDC+ W +LPP +  +R+++   KGRF+GDPS EFEH E K+V GEG++  EE+ET++IKEEDRL++VIA+ID+DV IVPR A+++ PTG++++NRSFEGL VSE+AK  SY HFREP  L + S +  A +DK+IDF+D++E D+P+ +W+LQF+RGS LV L+SLLW GYVFYH+P  + +GS+Y+G GE N+DLPFM
Sbjct:   31 MESSGLHLDIDYVGSSGVILSPEQKAALQTSLVILQSHNKFHKVYLWGKILGVKDDYFIAQGVSRDEMIEKKTFYSKDCVNWGLLPPATKAMRDQSKLAKGRFTGDPSYEFEHTETKKV-GEGEDAQEEEETISIKEEDRLSSVIADIDDDVKIVPRGAFVQTPTGEVIANRSFEGLIVSEAAKLCSYMHFREPKLLPEKSLLQKANMDKSIDFMDNLEEDIPKGAWSLQFERGSGLVSLRSLLWQGYVFYHIPGCKKYGSIYVGTGEKNLDLPFM 305
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BLAST of radial spoke head protein 9 homolog vs. Ensembl Cavefish
Match: rsph9 (radial spoke head protein 9 homolog [Source:NCBI gene;Acc:111190171])

HSP 1 Score: 251.906 bits (642), Expect = 8.485e-83
Identity = 124/277 (44.77%), Postives = 181/277 (65.34%), Query Frame = 1 
Query:   76 MNSSDLHLMIDYLGSSGILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPS-VEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            M+S  L+  +D +  SG+ LS E +A LQSSL+ILK    F +V+FWG I G K+DYF+ QG+G D++ D+K++YS +C+ W +LPP +   I E A   KGRF GDPS+++EH   +Q  G+      E+ +V + EE RLA  +  IDN+V +VPR AYIK   G    NRSFEGL+ SE+A+ S+Y HF E  + +K + M++A L+ +IDFLD +  D+P+ SW + F+ G+ +  +KSL+W G  F+++P +   G LYMG G  N+DL FM
Sbjct:    1 MDSDALYCSVDLVCGSGVTLSAEHRAALQSSLIILKRNMNFRRVLFWGKICGIKNDYFIAQGVGEDQIRDRKSIYSINCIDWHLLPPATKALIDEVAMAAKGRFMGDPSHKYEHETQEQEEGDC-----ERCSVKVNEEQRLAVTVFTIDNEVAVVPRGAYIKNLQGIAQPNRSFEGLNPSEAAELSNYLHFSESRKPRKKNIMEMANLNPSIDFLDPLSEDIPKGSWIVHFEHGNRVCTIKSLVWQGLTFFNLPMTPWHGYLYMGDGLKNVDLSFM 272
[Close]
BLAST of radial spoke head protein 9 homolog vs. Ensembl Cavefish
Match: rsph9 (radial spoke head protein 9 homolog [Source:NCBI gene;Acc:111190171])

HSP 1 Score: 248.825 bits (634), Expect = 1.705e-81
Identity = 123/277 (44.40%), Postives = 178/277 (64.26%), Query Frame = 1 
Query:   76 MNSSDLHLMIDYLGSSGILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPS-VEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            M+S  L+  +D +  SG+ LS E +A LQSSL+ILK    F +V+FWG I G K+DYF+ QG+G D++ D+K++YS +C+ W +LPP +   I E A   KGRF GDPS+++EH   +Q  G       +   V + EE RLA  +  IDN+V +VPR AYIK   G    NRSFEGL+ SE+A+ S+Y HF E  + +K + M++A L+ +IDFLD +  D+P+ SW + F+ G+ +  +KSL+W G  F+++P +   G LYMG G  N+DL FM
Sbjct:    1 MDSDALYCSVDLVCGSGVTLSAEHRAALQSSLIILKRNMNFRRVLFWGKICGIKNDYFIAQGVGEDQIRDRKSIYSINCIDWHLLPPATKALIDEVAMAAKGRFMGDPSHKYEHETQEQEEGG------QTTKVKVNEEQRLAVTVFTIDNEVAVVPRGAYIKNLQGIAQPNRSFEGLNPSEAAELSNYLHFSESRKPRKKNIMEMANLNPSIDFLDPLSEDIPKGSWIVHFEHGNRVCTIKSLVWQGLTFFNLPMTPWHGYLYMGDGLKNVDLSFM 271
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BLAST of radial spoke head protein 9 homolog vs. Ensembl Cavefish
Match: ENSAMXT00000056509.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000668.1:119351:123485:1 gene:ENSAMXG00000033044.1 transcript:ENSAMXT00000056509.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 214.157 bits (544), Expect = 3.182e-68
Identity = 111/262 (42.37%), Postives = 161/262 (61.45%), Query Frame = 1 
Query:  121 SGILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPS-VEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            +G+ LS E +A LQSSL+ILK    F +V+FWG I            +G D++ D+K++YS +C+ W +LPP +   I E A   KGRF GDPS+++EH   +Q  G       +   V + EE RLA  +  IDN+V +VPR AYIK   G    NRSFEGL+ SE+A+ S+Y HF E  + +K + M++A L+ +IDFLD +  D+P+ SW + F+ G+ +  +KSL+W G  F+++P +   G LYMG G  N+DL FM
Sbjct:    5 AGVTLSAEHRAALQSSLIILKRNMNFRRVLFWGKI-----------WVGEDQIRDRKSIYSINCIDWHLLPPATKALIDEVAMAAKGRFMGDPSHKYEHETQEQEEGG------QTTKVKVNEEQRLAVTVFTIDNEVAVVPRGAYIKNLQGIAQPNRSFEGLNPSEAAELSNYLHFSESRKPRKKNIMEMANLNPSIDFLDPLSEDIPKGSWIVHFEHGNRVCTIKSLVWQGLTFFNLPMTPWHGYLYMGDGLKNVDLSFM 249
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BLAST of radial spoke head protein 9 homolog vs. Ensembl Cavefish
Match: ENSAMXT00000031004.1 (pep primary_assembly:Astyanax_mexicanus-2.0:25:13627877:13630896:-1 gene:ENSAMXG00000033656.1 transcript:ENSAMXT00000031004.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 113.235 bits (282), Expect = 3.239e-30
Identity = 53/126 (42.06%), Postives = 80/126 (63.49%), Query Frame = 1 
Query:   76 MNSSDLHLMIDYLGSSGILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPS-VEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDE 450
            M+   L+  +D L   G+ L+ E +A LQ+S +ILK   KF++V+FWG I G + DY++ QG+G DE+ D+K  YS+DC+ W +LPP +   I E A   KGRF GDPS+++E   +++  G   E
Sbjct:    1 MDLEALYTSLDLLSRFGVTLNAEHRAALQTSFIILKTHMKFSRVLFWGKIFGLERDYYIAQGLGEDEIKDRKYFYSQDCIDWYLLPPATETLIDEVAVAAKGRFMGDPSHKYEQGTMEKEEGADTE 126
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BLAST of radial spoke head protein 9 homolog vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001996.1 (pep scaffold:Pmarinus_7.0:GL482300:2480:5142:1 gene:ENSPMAG00000001811.1 transcript:ENSPMAT00000001996.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 164.851 bits (416), Expect = 4.570e-51
Identity = 76/145 (52.41%), Postives = 102/145 (70.34%), Query Frame = 1 
Query:  478 IKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMS---WALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            +KEEDRLAA I  I+++  +VPR A+++ P G++  N +F+GLS+ E+ K  SY H+R P  L K S ++ A LD A+DFLD IE D+P  +   W+LQ +RG +LVIL+SLLWPG  FYHVP +   G +YMG GE NIDLPFM
Sbjct:    1 VKEEDRLAATITAIEHEAAVVPRGAFMRTPLGRVQRNPTFQGLSLEEAGKLRSYLHWRRPERLPKLSLLERAELDPALDFLDPIEHDVPAGAAGCWSLQLERGGALVILRSLLWPGLTFYHVPGTPRHGYIYMGLGEKNIDLPFM 145
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BLAST of radial spoke head protein 9 homolog vs. Ensembl Nematostella
Match: EDO27772 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T8C0])

HSP 1 Score: 266.929 bits (681), Expect = 2.172e-89
Identity = 128/243 (52.67%), Postives = 176/243 (72.43%), Query Frame = 1 
Query:  193 KFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPSVEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEG---DEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEG--LSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPE-MSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            KF KV +WG I G K+DYF++QG+G DE+ ++KTLYS+DC+ W +LP  + ++R   +F KGRF+GDPS E+EH E  ++  +     E  EE+ T+ IKEEDRLA  I  ID DV IVPR +YI+  TG ++ NRSFEG  LSV+++ K +SYFHFR+PV   + S +  A LDKA+DFLD I+TD+P+  SW++QF+RGS LV L+SL W G+ F+HVPE+R +G +Y+G G  N+DLPFM
Sbjct:    3 KFEKVRYWGKILGVKNDYFIIQGVGKDELKERKTLYSQDCIHWGLLPVVTDKMRHDCSFVKGRFTGDPSYEYEHTETVKIDNQDGEKTEGGEEENTILIKEEDRLATTIEAIDYDVRIVPRGSYIRTATGAVIENRSFEGNWLSVADAGKLASYFHFRDPVLFNQKSLLQKAELDKAVDFLDPIDTDVPKGGSWSIQFERGSGLVSLRSLHWLGFTFFHVPETRKYGMVYIGTGNKNLDLPFM 245
[Close]
BLAST of radial spoke head protein 9 homolog vs. Ensembl Medaka
Match: rsph9 (radial spoke head component 9 [Source:NCBI gene;Acc:101156546])

HSP 1 Score: 237.269 bits (604), Expect = 5.242e-77
Identity = 121/279 (43.37%), Postives = 178/279 (63.80%), Query Frame = 1 
Query:   76 MNSSDLHLMIDYLGSSGILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPS---VEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            M+ + L   ++++  SG +L+ E++  LQ+SLVI+K   KF +++FWG I G + DYFV QG G DEMTD+K+LYS +C+ W++LP  +   VE   KAA  +GRF G+PS  +EH +V Q  G+     E+++  ++ EE RLA  +  ID +  +VPR A+I+   G + +NRSF GLS SE+ +  ++ HFR+P  LQK      A    AIDFLD +  D+P+ SW+LQF+    + +L+SLLW G  FYHVP +   G +Y+G G  N+DLPFM
Sbjct:    1 MDFNSLPYSLEFVAGSGCILTLEERTALQTSLVIVKTNYKFQRILFWGKILGLQHDYFVAQGRGEDEMTDRKSLYSLNCVDWLLLPSVTDAMVEEVSKAA--QGRFKGNPSFVYEH-KVSQQEGK-----EQEKVFSVTEEKRLAVTVHHIDEEASVVPRGAFIQSLRGLVQTNRSFGGLSESEARRLENFMHFRKPKTLQKKPIPVKAEWAPAIDFLDVLSEDIPKGSWSLQFECARRVCVLRSLLWLGLTFYHVPMTPQHGYIYVGLGMKNLDLPFM 271
[Close]
BLAST of radial spoke head protein 9 homolog vs. Planmine SMEST
Match: SMESG000046857.1 (SMESG000046857.1)

HSP 1 Score: 563.533 bits (1451), Expect = 0.000e+0
Identity = 275/276 (99.64%), Postives = 276/276 (100.00%), Query Frame = 1 
Query:   76 MNSSDLHLMIDYLGSSGILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPSVEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            MNSSDLHLMIDYLGSSGILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPS+EIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM
Sbjct:    1 MNSSDLHLMIDYLGSSGILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPSLEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 276
[Close]
BLAST of radial spoke head protein 9 homolog vs. Planmine SMEST
Match: SMESG000078411.1 (SMESG000078411.1)

HSP 1 Score: 363.229 bits (931), Expect = 1.503e-126
Identity = 169/276 (61.23%), Postives = 213/276 (77.17%), Query Frame = 1 
Query:   76 MNSSDLHLMIDYLGSSGILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSIKGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPSVEIREKAAFFKGRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDNDVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKSSFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVFYHVPESRNFGSLYMGYGELNIDLPFM 903
            MN  + HL IDY+GSSGI+L+PEQKA LQ+SL ILK+EQKF KV FWGS+KG K DY++ QGIG DEM +K+T+YSKDC+ W ILP  + E +  + FFK RF+GDPSNEFE+I   +   +  E  EE+E + +KEEDRL+ ++ EID +VFIVPR AY++LPTGQIVSNR +EGL   +S K SSY HFR+P  L   + M+ AR DKAIDFLD IE D+P++SW+LQ DRG+ LVILKSL+WPGYVFY +P+ + FGS+Y GYGE NIDLPFM
Sbjct:    1 MNCVNFHLTIDYVGSSGIVLTPEQKAALQTSLTILKSEQKFFKVNFWGSLKGNKGDYYIAQGIGKDEMYEKRTIYSKDCMNWNILPAYTEEHKNLSKFFKERFTGDPSNEFEYINYIEKTDDKGETIEEEEILRLKEEDRLSVLVDEIDKEVFIVPRGAYVRLPTGQIVSNRLYEGLPAIDSLKLSSYLHFRQPYALPLKTLMERARYDKAIDFLDPIEEDIPKISWSLQLDRGTGLVILKSLVWPGYVFYQIPDHKAFGSVYFGYGERNIDLPFM 276
The following BLAST results are available for this feature:
BLAST of radial spoke head protein 9 homolog vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
RSPH94.233e-9750.19radial spoke head component 9 [Source:HGNC Symbol;... [more]
RSPH92.701e-5741.48radial spoke head component 9 [Source:HGNC Symbol;... [more]
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BLAST of radial spoke head protein 9 homolog vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of radial spoke head protein 9 homolog vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG318036.870e-4936.13gene:FBgn0051803 transcript:FBtr0081010[more]
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BLAST of radial spoke head protein 9 homolog vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rsph98.527e-10048.74radial spoke head component 9 [Source:NCBI gene;Ac... [more]
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BLAST of radial spoke head protein 9 homolog vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rsph97.538e-4954.23radial spoke head 9 homolog [Source:NCBI gene;Acc:... [more]
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BLAST of radial spoke head protein 9 homolog vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Rsph99.761e-9247.47radial spoke head 9 homolog (Chlamydomonas) [Sourc... [more]
Rsph91.987e-1846.84radial spoke head 9 homolog (Chlamydomonas) [Sourc... [more]
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BLAST of radial spoke head protein 9 homolog vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q5TYW6|RSPH9_DANRE6.437e-9948.74Radial spoke head protein 9 homolog OS=Danio rerio... [more]
sp|Q9H1X1|RSPH9_HUMAN2.033e-9650.19Radial spoke head protein 9 homolog OS=Homo sapien... [more]
sp|Q2KIU7|RSPH9_BOVIN1.154e-9350.57Radial spoke head protein 9 homolog OS=Bos taurus ... [more]
sp|Q9D9V4|RSPH9_MOUSE6.827e-9147.47Radial spoke head protein 9 homolog OS=Mus musculu... [more]
sp|Q6DFN5|RSPH9_XENTR3.130e-7644.40Radial spoke head protein 9 homolog OS=Xenopus tro... [more]
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BLAST of radial spoke head protein 9 homolog vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3KIZ64.497e-12564.86radial spoke head protein 9 homolog OS=Lingula ung... [more]
A0A504YY281.243e-12459.42Radial spoke head protein 9 OS=Fasciola gigantica ... [more]
Q5D8R21.434e-11858.33Radial spoke head protein 9 isoform 2 (Fragment) O... [more]
A0A0B7ABU33.439e-11859.06Uncharacterized protein OS=Arion vulgaris OX=10286... [more]
A0A210QAA35.428e-11759.78Radial spoke head protein 9-like OS=Mizuhopecten y... [more]
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BLAST of radial spoke head protein 9 homolog vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
rsph98.485e-8344.77radial spoke head protein 9 homolog [Source:NCBI g... [more]
rsph91.705e-8144.40radial spoke head protein 9 homolog [Source:NCBI g... [more]
ENSAMXT00000056509.13.182e-6842.37pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000031004.13.239e-3042.06pep primary_assembly:Astyanax_mexicanus-2.0:25:136... [more]
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BLAST of radial spoke head protein 9 homolog vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000001996.14.570e-5152.41pep scaffold:Pmarinus_7.0:GL482300:2480:5142:1 gen... [more]
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BLAST of radial spoke head protein 9 homolog vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of radial spoke head protein 9 homolog vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO277722.172e-8952.67Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of radial spoke head protein 9 homolog vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rsph95.242e-7743.37radial spoke head component 9 [Source:NCBI gene;Ac... [more]
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BLAST of radial spoke head protein 9 homolog vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000046857.10.000e+099.64SMESG000046857.1[more]
SMESG000078411.11.503e-12661.23SMESG000078411.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012136 ID=SMED30012136|Name=radial spoke head protein 9 homolog|organism=Schmidtea mediterranea sexual|type=transcript|length=947bp
GTTTGAATAAATTTGTTTATCTGATACAATTAATGATATTACTATAACAA
CAGCGCTGCAGTAAATTGCTTAACGATGAATTCGTCTGATTTGCACCTGA
TGATAGATTATCTGGGAAGCAGTGGAATTCTGCTGTCGCCAGAACAAAAA
GCTGTTTTACAATCATCTTTGGTAATTTTGAAAAATGAACAAAAATTTAA
CAAAGTCGTTTTCTGGGGCTCGATAAAAGGAACAAAATCTGACTACTTTG
TTGTGCAAGGAATTGGAGCCGATGAAATGACTGACAAGAAAACTCTTTAC
AGTAAGGATTGCTTGAAATGGGTAATTCTTCCCCCTCCATCGGTGGAGAT
TCGAGAGAAAGCAGCTTTTTTTAAAGGCAGATTCTCGGGAGATCCTAGTA
ACGAATTTGAACATATCGAAGTGAAACAAGTTCCTGGGGAAGGGGATGAA
GTTCATGAAGAGCAAGAAACGGTAACTATTAAAGAGGAAGACAGACTAGC
TGCCGTTATCGCAGAAATCGACAATGATGTATTCATTGTTCCAAGAGCGG
CCTATATTAAACTTCCAACTGGTCAAATTGTTTCCAATCGCTCATTTGAA
GGTTTGTCTGTGTCTGAATCGGCTAAGCGGTCATCATATTTTCATTTCCG
TGAGCCAGTCGAATTACAAAAGAGCAGTTTTATGGACCTGGCTAGGCTGG
ATAAAGCGATAGATTTTCTCGATTCTATTGAAACTGATTTGCCGGAAATG
AGTTGGGCATTGCAATTTGATCGCGGCAGCAGTTTGGTTATCTTGAAAAG
TCTTTTGTGGCCTGGATATGTTTTCTATCATGTTCCTGAATCTAGAAATT
TCGGGTCCCTCTATATGGGCTATGGAGAGCTGAATATCGATTTGCCTTTT
ATGTTGTGATGATTTTTAATATTATTGATAATAAATAAATAAATGAG
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protein sequence of SMED30012136-orf-1

>SMED30012136-orf-1 ID=SMED30012136-orf-1|Name=SMED30012136-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=278bp
MNSSDLHLMIDYLGSSGILLSPEQKAVLQSSLVILKNEQKFNKVVFWGSI
KGTKSDYFVVQGIGADEMTDKKTLYSKDCLKWVILPPPSVEIREKAAFFK
GRFSGDPSNEFEHIEVKQVPGEGDEVHEEQETVTIKEEDRLAAVIAEIDN
DVFIVPRAAYIKLPTGQIVSNRSFEGLSVSESAKRSSYFHFREPVELQKS
SFMDLARLDKAIDFLDSIETDLPEMSWALQFDRGSSLVILKSLLWPGYVF
YHVPESRNFGSLYMGYGELNIDLPFML*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0001534radial spoke
GO:0005737cytoplasm
GO:0005856cytoskeleton
GO:0005929cilium
GO:0042995cell projection
Vocabulary: biological process
TermDefinition
GO:0060271cilium morphogenesis
GO:0003341cilium movement
GO:0035082axoneme assembly
GO:0044458motile cilium assembly
GO:0060294cilium movement involved in cell motility
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR006802Radial_spoke
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006802Radial spokehead-like proteinPFAMPF04712Radial_spokecoord: 9..108
e-value: 2.2E-7
score: 30.2
NoneNo IPR availablePANTHERPTHR22069MITOCHONDRIAL RIBOSOMAL PROTEIN S18coord: 1..277
NoneNo IPR availablePANTHERPTHR22069:SF0RADIAL SPOKE HEAD PROTEIN 9 HOMOLOGcoord: 1..277