NADH-quinone oxidoreductase subunit D

Overview
NameNADH-quinone oxidoreductase subunit D
Smed IDSMED30012038
Length (bp)1121
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012038

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 34

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30012038h1SMcG0020718 Contig4368GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30012038h1SMcG0020718 Contig4368GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30012038h1SMcG0020717 Contig4368GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30012038h1SMcG0020717 Contig4368GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30012038h1SMcG0021503 Contig4368GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30012038h1SMcG0021503 Contig4368GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
Smed sexual biotypeSMED30012038h1SMcG0020718 Contig41911uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012038h1SMcG0020718 Contig41911newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012038h1SMcG0020718 Contig36994uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012038h1SMcG0020718 Contig36994newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012038h1SMcG0020718 Contig16197newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30012038h1SMcG0020718 Contig16197uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30012038h1SMcG0020719 dd_Smed_v4_311_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30012038h1SMcG0020719 dd_Smed_v4_311_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30012038h1SMcG0020719 dd_Smed_v4_311_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30012038h1SMcG0020719 dd_Smed_v4_311_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30012038h1SMcG0020719 dd_Smed_v4_311_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30012038h1SMcG0020719 dd_Smed_v4_311_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30012038h1SMcG0020718 dd_Smed_v4_311_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30012038h1SMcG0020718 dd_Smed_v4_311_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30012038h1SMcG0020718 dd_Smed_v4_311_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30012038h1SMcG0020718 dd_Smed_v4_311_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30012038h1SMcG0020718 dd_Smed_v4_311_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30012038h1SMcG0020718 dd_Smed_v4_311_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30012038h1SMcG0020718 dd_Smed_v6_311_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30012038h1SMcG0020718 dd_Smed_v6_311_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pigment cellSMED30012038h1SMcG0020718 dd_Smed_v6_311_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30012038h1SMcG0020718 dd_Smed_v6_311_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30012038h1SMcG0020719 dd_Smed_v6_311_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30012038h1SMcG0020719 dd_Smed_v6_311_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pigment cellSMED30012038h1SMcG0020719 dd_Smed_v6_311_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30012038h1SMcG0020719 dd_Smed_v6_311_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30012038h1SMcG0020719 dd_Smed_v4_311_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30012038h1SMcG0020718 dd_Smed_v4_311_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of NADH-quinone oxidoreductase subunit D vs. TrEMBL
Match: A0A151ICQ4 (Uncharacterized protein OS=Cyphomyrmex costatus OX=456900 GN=ALC62_11109 PE=4 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 1.532e-60
Identity = 89/238 (37.39%), Postives = 134/238 (56.30%), Query Frame = 1 
Query:  451 PGAMYHAKRMAKAIYSLKMVLFQGQMTLTARELKGLTELALFVALVYGRFWHEAPLASHAHLNDA*MLRLLQTYP--N*TVADAAFSALC-HLWFFSEHLVGLAFFDDRVESHVKKAMVANLQLPKTRSALHRVDSSDADF----NNLET--------FVTEITNRLFELVSATGVEEFRSFLLKEPEA*EADASYQKLYERVKMI*VVNDSAERGIALIEKYNESITEDEDQKQFLL 1119
            PGA++HA+ ++KAIY LKM LF+ +  LT  E+  L E  LF+   Y ++W+ AP A  A  ND  M++   TY   N  +  A+   L  HLW+ SE+L G+A  DDRV + +K+ M+      K  +  H  D++   F    NN+++        FV+  + R+FE +S +      SFL  +P+  + +  + K  E  K + VVND AERG+ALIE YN  +T +E+QKQFL+
Sbjct:  486 PGAVHHARWLSKAIYGLKMYLFREEFRLTNYEVTALCEFCLFIVTFYIKWWYTAPFAIQAPYNDLMMIQDFITYEAINDKILKASQCKLANHLWYLSENLAGIALMDDRVPNELKRKMI------KAMNERHGTDANSNKFVISENNIKSLAVKDLSDFVSRKSRRIFEEMSISC-----SFLETDPKEWDNNNDFLKCVEVFKSMKVVNDIAERGVALIEHYNSILTRNEEQKQFLV 712

HSP 2 Score: 119.013 bits (297), Expect = 1.532e-60
Identity = 59/134 (44.03%), Postives = 80/134 (59.70%), Query Frame = 3 
Query:   45 WKLRDVVQGLVFDTTSSNTGIHKGACVLIE*ALVRELVNIGCYHHVLEVVLSSVFTVLFGGTGGPEVGLL*RFKNKWSYIKQNDYSHAKNELFIS-VTEILRKEMVAFYTNAITYRQPREDYLELLRLCLVFLG 443
            W + D V+ L FDTT SNTG  KG CVLIE +L R+L  +GC HH+LE+VL +VF + +  T  P V +  RF+  W  I    +    N+  IS V    + E++ F  N +  RQPR+DY ELL L ++FLG
Sbjct:  341 WNVEDRVKALCFDTTPSNTGPDKGTCVLIEKSLKRKLFYLGCRHHILELVLKNVFQIYWPATSAPTVSIFIRFQENWDRIDVTKFKPGINDPKISQVLSDKKDEILNFTLNFLQIRQPRDDYKELLELTIIFLG 474
[Close]
BLAST of NADH-quinone oxidoreductase subunit D vs. TrEMBL
Match: A0A151IT70 (Uncharacterized protein (Fragment) OS=Trachymyrmex cornetzi OX=471704 GN=ALC57_17860 PE=4 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 7.324e-59
Identity = 82/229 (35.81%), Postives = 128/229 (55.90%), Query Frame = 1 
Query:  448 APGAMYHAKRMAKAIYSLKMVLFQGQMTLTARELKGLTELALFVALVYGRFWHEAPLASHAHLNDA*MLRLLQTYP--N*TVADAAFSALC-HLWFFSEHLVGLAFFDDRVESHVKKAMVANLQLPKTRSALHR--VDSSDADFNNLETFVTEITNRLFELVSATGVEEFRSFLLKEPEA*EADASYQKLYERVKMI*VVNDSAERGIALIEKYNESITEDEDQKQFLL 1119
            +PGA + A+ MAK IY +K+ LF+ Q  + A EL GL +  LF+  +Y + W+ +     A +ND  +L+ L  Y   N TV+ AA+     HLW+ S+ L GL+ FD R+ +  +K M  +L+   T     R  VD    D N +  F+TE + ++F      G+    +FL K+P   E +  + K   R+  + VVND AERG++LI+ +N  +T+ E+QKQ+LL
Sbjct:  264 SPGAYHRARWMAKLIYCMKIYLFRSQFKVRASELSGLRQFNLFLIKIYLKKWYTSQCPILAPVNDLELLKDLIQYKSINPTVSKAAYDTFSRHLWYLSDSLAGLSLFDSRISNEERKKMTNSLRKDGTEIPPKRIIVDKDTIDVNEISDFITENSKQIF---ITNGIS--LNFLHKDPSLWEENEEFIKSRYRIGQLKVVNDVAERGVSLIQNFNSVLTKQEEQKQYLL 487

HSP 2 Score: 121.324 bits (303), Expect = 7.324e-59
Identity = 58/134 (43.28%), Postives = 82/134 (61.19%), Query Frame = 3 
Query:   45 WKLRDVVQGLVFDTTSSNTGIHKGACVLIE*ALVRELVNIGCYHHVLEVVLSSVFTVLFGGTGGPEVGLL*RFKNKWSYIKQNDYSHA-KNELFISVTEILRKEMVAFYTNAITYRQPREDYLELLRLCLVFLG 443
            W L D V  + FDTT+SNTG ++GACV +E  L  EL ++ C HH+ E+++ SVF +LFG + GP + +  RF+  W  I Q  Y  A K++   +    ++ EM+ F  N +   QPREDY ELL L L+FLG
Sbjct:  122 WNLSDTVTSMCFDTTASNTGKNRGACVELEKKLGHELFSLACRHHIHELIVGSVFDILFGPSSGPNIKIFQRFQESWESIDQEKYESALKDKKISTFLAPIQNEMIDFIRNQLLLFQPREDYKELLILSLIFLG 255
[Close]
BLAST of NADH-quinone oxidoreductase subunit D vs. TrEMBL
Match: A0A151IV02 (Uncharacterized protein OS=Trachymyrmex cornetzi OX=471704 GN=ALC57_16486 PE=4 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 8.458e-58
Identity = 81/229 (35.37%), Postives = 127/229 (55.46%), Query Frame = 1 
Query:  448 APGAMYHAKRMAKAIYSLKMVLFQGQMTLTARELKGLTELALFVALVYGRFWHEAPLASHAHLNDA*MLRLLQTYP--N*TVADAAFSALC-HLWFFSEHLVGLAFFDDRVESHVKKAMVANLQLPKTRSALHR--VDSSDADFNNLETFVTEITNRLFELVSATGVEEFRSFLLKEPEA*EADASYQKLYERVKMI*VVNDSAERGIALIEKYNESITEDEDQKQFLL 1119
            +PGA + A+ MAK IY +K+ LF+    + A EL GL +  LF+  +Y + W+ +     A +ND  +L+ L  Y   N TV+ AA+     HLW+ S+ L GL+ FD R+ +  +K M  +L+   T     R  VD    D N +  F+TE + ++F      G+    +FL K+P   E +  + K   R+  + VVND AERG++LI+ +N  +T+ E+QKQ+LL
Sbjct:  221 SPGAYHRARWMAKLIYCMKIYLFRSLFKVRASELSGLRQFNLFLIKIYLKKWYTSQCPILAPVNDLELLKDLIQYKSINPTVSKAAYDTFSRHLWYLSDSLAGLSLFDSRISNEERKKMTNSLRKDGTEIPPKRIIVDKDTIDVNEISDFITENSKQIF---ITNGIS--LNFLHKDPSLWEENEEFIKSRYRIGQLKVVNDVAERGVSLIQNFNSVLTKQEEQKQYLL 444

HSP 2 Score: 120.939 bits (302), Expect = 8.458e-58
Identity = 58/134 (43.28%), Postives = 82/134 (61.19%), Query Frame = 3 
Query:   45 WKLRDVVQGLVFDTTSSNTGIHKGACVLIE*ALVRELVNIGCYHHVLEVVLSSVFTVLFGGTGGPEVGLL*RFKNKWSYIKQNDYSHA-KNELFISVTEILRKEMVAFYTNAITYRQPREDYLELLRLCLVFLG 443
            W L D V  + FDTT+SNTG ++GACV +E  L  EL ++ C HH+ E+++ SVF +LFG + GP + +  RF+  W  I Q  Y  A K++   +    ++ EM+ F  N +   QPREDY ELL L L+FLG
Sbjct:   79 WNLSDTVTSMCFDTTASNTGKNRGACVELEKKLGHELFSLACRHHIHELIVGSVFDILFGPSSGPNIKIFQRFQESWESIDQEKYESALKDKKISTFLAPIQNEMIDFIRNQLLLFQPREDYKELLILSLIFLG 212
[Close]
BLAST of NADH-quinone oxidoreductase subunit D vs. TrEMBL
Match: J9LU94 (Uncharacterized protein OS=Acyrthosiphon pisum OX=7029 PE=4 SV=2)

HSP 1 Score: 140.584 bits (353), Expect = 2.302e-56
Identity = 90/228 (39.47%), Postives = 130/228 (57.02%), Query Frame = 1 
Query:  451 PGAMYHAKRMAKAIYSLKMVLFQGQMTLTARELKGLTELALFVALVYGRFWHEAPLASHAHLNDA*MLRLLQTYP--N*TVADAAFSALC-HLWFFSEHLVGLAFFDDRVESHVKKAMVANLQLPKTRSALHR--VDSSDADFNNLETFVTEITNRLFELVSATGVEEFRSFLLKEPEA*EADASYQKLYERVKMI*VVNDSAERGIALIEKYNESITEDEDQKQFLL 1119
            PGA++ A+ MAK IYSLK+ LF+ Q  LTAREL  L    +FV  VY ++W+ A     A  ND  +L+ L  Y   N  + +AA  +   HLW+ SE L+GLAFFD +V S +K  MV +L        L R  V+++      L  FV++ +  LF     T ++  + FL + P+  E    +    +RV+ + VVND+AERGI+LI+ +N  IT  E+QKQ+LL
Sbjct:  479 PGALHRARWMAKLIYSLKIFLFRSQFKLTARELSALEAFNVFVIQVYIKYWYTASSGELAPYNDLNLLKELDNYKKSNIVIGNAAAKSFSRHLWYLSETLIGLAFFDSKVSSEMKVNMVKSLVKKGADFPLRRITVETTAVQTVQLSDFVSKNSLILF-----TALDIPQDFLNQNPDTWENHKDFIDGCKRVQHLKVVNDAAERGISLIQTFNGIITNQEEQKQYLL 701

HSP 2 Score: 108.997 bits (271), Expect = 2.302e-56
Identity = 52/134 (38.81%), Postives = 82/134 (61.19%), Query Frame = 3 
Query:   45 WKLRDVVQGLVFDTTSSNTGIHKGACVLIE*ALVRELVNIGCYHHVLEVVLSSVFTVLFGGTGGPEVGLL*RFKNKWSYIKQNDYSHAKNELFISVTEILRKEMVAFYTN-AITYRQPREDYLELLRLCLVFLG 443
            W L + +  + FDTT+SN+G+  GACVL+E  + R+L+++ C HH+LE+++ SVF  L G + GP + +  RF  KW+ I  + Y     E  ++     +K ++  Y N  +   QPR+DY ELL+L L+FLG
Sbjct:  334 WNLIERINFMSFDTTASNSGVKAGACVLLEKKIKRDLLSLACRHHILELIIESVFNALMGASSGPNIKIFQRFAEKWNEIDVDKYKSGIIEDTVASKLNPQKYVLVKYINDQLATFQPRDDYKELLQLSLIFLG 467
[Close]
BLAST of NADH-quinone oxidoreductase subunit D vs. TrEMBL
Match: A0A2S2PCY9 (Uncharacterized protein OS=Schizaphis graminum OX=13262 GN=g.57453 PE=4 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 1.040e-55
Identity = 89/228 (39.04%), Postives = 129/228 (56.58%), Query Frame = 1 
Query:  451 PGAMYHAKRMAKAIYSLKMVLFQGQMTLTARELKGLTELALFVALVYGRFWHEAPLASHAHLNDA*MLRLLQTYP--N*TVADAAFSALC-HLWFFSEHLVGLAFFDDRVESHVKKAMVANLQLPKTRSALHR--VDSSDADFNNLETFVTEITNRLFELVSATGVEEFRSFLLKEPEA*EADASYQKLYERVKMI*VVNDSAERGIALIEKYNESITEDEDQKQFLL 1119
            PGA++ A+ MAK IYSLK+ LF+ Q  LTAREL  L    +FV  VY ++W+ A     A  ND  +L+ L  Y   N  + +AA  +   HLW+ SE L+GLAFFD +V S +K  MV +L        L R  V+++      L  FV++ +  LF+ +        + FL + P+  E    +    +RV+ + VVND+AERGI+LI+ +N  IT  E+QKQ+LL
Sbjct:  504 PGALHRARWMAKLIYSLKIFLFRSQFKLTARELSALEAFNVFVIQVYIKYWYTASSGELAPYNDLNLLKELDNYKKSNIVIGNAAAKSFSRHLWYLSETLIGLAFFDSKVSSEMKVNMVKSLVKKGADFPLRRITVETTAVQTVQLSDFVSKNSLILFKALDIP-----QDFLNQNPDTWENHKDFIDGCKRVQHLKVVNDAAERGISLIQTFNGIITNQEEQKQYLL 726

HSP 2 Score: 108.227 bits (269), Expect = 1.040e-55
Identity = 52/134 (38.81%), Postives = 81/134 (60.45%), Query Frame = 3 
Query:   45 WKLRDVVQGLVFDTTSSNTGIHKGACVLIE*ALVRELVNIGCYHHVLEVVLSSVFTVLFGGTGGPEVGLL*RFKNKWSYIKQNDYSHAKNELFISVTEILRKEMVAFYTN-AITYRQPREDYLELLRLCLVFLG 443
            W L + +  + FDTT+SN+G+  GACVL+E  + R+L+++ C HH+LE+++ SVF  L G + GP + +  RF  KW+ I    Y     E  ++     +K ++  Y N  +   QPR+DY ELL+L L+FLG
Sbjct:  359 WNLIERIHFMSFDTTASNSGVKAGACVLLEKKIKRDLLSLACRHHILELIIESVFNALMGASSGPNIKIFQRFAEKWNEIDVEKYESGIIEDAVASKLNPQKYVLVKYINDQLATFQPRDDYKELLQLSLIFLG 492
[Close]
BLAST of NADH-quinone oxidoreductase subunit D vs. Planmine SMEST
Match: SMESG000028508.1 (SMESG000028508.1)

HSP 1 Score: 88.9669 bits (219), Expect = 5.243e-35
Identity = 50/149 (33.56%), Postives = 79/149 (53.02%), Query Frame = 1 
Query:  448 APGAMYHAKRMAKAIYSLKMVLFQGQMTLTARELKGLTELALFVALVYGRFWHEAPLASHAHLNDA*MLRLLQTYP--N*TVADAAFSALC-HLWFFSEHLVGLAFFDDRVESHVKKAMVANLQLPKTRSALHRVDSSDADFNNLETFV 885
            APGAM HA+ M+K +YSLK+ +F+ Q  L   E+KGL ++  F   +Y + W  AP A+    ND   L+ L  Y   +  ++ AA + +  HLW+ SE LVGLA FD  + +  K+ +V+ ++     +       S  D N   + +
Sbjct:  883 APGAMQHARWMSKVLYSLKVYMFRSQFDLRPTEVKGLRDICTFSVSLYIKVWFTAPCAATGPRNDLTFLQALIDYEVVHPAISKAASAKMAKHLWYISEELVGLALFDKDLSTTTKRELVSAIKFSSQINMHQSSSESSIDKNKFPSVI 1031

HSP 2 Score: 78.1814 bits (191), Expect = 5.243e-35
Identity = 38/117 (32.48%), Postives = 65/117 (55.56%), Query Frame = 3 
Query:   96 NTGIHKGACVLIE*ALVRELVNIGCYHHVLEVVLSSVFTVLFGGTGGPEVGLL*RFKNKWSYIKQNDYSHAKNELFISVTEI-LRKEMVAFYTNAITYRQPREDYLELLRLCLVFLG 443
            N  +  GACVL+E  L   L+++ C HH+ E+V+ +VF    G +   EV +  RF++ W +I    YS   ++  ++   +  R +++ F  + +   QPR+DY E L L ++FLG
Sbjct:  756 NRSLANGACVLLEQMLGINLLSLACRHHIFELVIGAVFQTCMGPSSAQEVLMFKRFQSCWQFIDPASYSTGMDDKDVAAVLMDTRNKLLEFAESQLQIMQPRDDYREFLELSIIFLG 872
[Close]
BLAST of NADH-quinone oxidoreductase subunit D vs. Planmine SMEST
Match: SMESG000067123.1 (SMESG000067123.1)

HSP 1 Score: 98.5969 bits (244), Expect = 8.268e-24
Identity = 68/187 (36.36%), Postives = 97/187 (51.87%), Query Frame = 1 
Query:  562 ELALFVALVYGRFWHEAPLASHAHLNDA*MLRLLQTYPN*TVADAAFSA-LCHLWFFSEHLVGLAFFDDRVESHVKKAMVANLQLPKTRSALHRVDSSDADFNNLETFVTEITNRLFELVSATGVEEFRSFLLKEPEA*EADASYQKLYERVKMI*VVNDSAERGIALIEKYNESITEDEDQKQFLL 1119
            E +LFV  VY ++W E+P+A  A   D  +L  L  YPN  ++ A  +A   HLW+ SE L+  AFFDD V +  K+ MV          AL  V+ SD     ++ F    T +L   V+ +    F + L           S   L E V+ + VVND  ERG+ LI+++N S+T DE+QKQ+LL
Sbjct:   37 EASLFVTRVYVKYWFESPVAHCAPQYDLALLCSLSEYPNTEISKATTTASQSHLWYLSETLI--AFFDDAVTTEDKRLMVV---------ALQEVEGSDEPLKRIQPFQHPTTKKLHNFVTKS-TSNFFTIL---------GISQNFLQELVQSVRVVNDLVERGVGLIQEFNSSLTRDEEQKQYLL 202
[Close]
BLAST of NADH-quinone oxidoreductase subunit D vs. Planmine SMEST
Match: SMESG000006554.1 (SMESG000006554.1)

HSP 1 Score: 85.8853 bits (211), Expect = 7.140e-22
Identity = 60/195 (30.77%), Postives = 92/195 (47.18%), Query Frame = 1 
Query:  544 ELKGLTELALFVALVYGRFWHEAPLASHAHLNDA*MLRLLQTYP--N*TVADAAFSAL-CHLWFFSEHLVGLAFFDDRVESHVKKAMVANLQLPKTRSALHRVDSSDADFNNLETFVTEITNRLFELVSATGVEEFRSFLLKEPEA*EADASYQKLYERVKMI*VVNDSAERGIALIEKYNESITEDEDQKQFLL 1119
            EL  L  + LF   +Y + W  AP +  A  ND  ML+ L+ +   +  VA  A   +  HLW+ SE L+GLA F   V    K AMV  L+  K ++ L RVD         +T    +T R   L +A  ++   +FL  +P        Y    +++  + V+ND A+R + L   +N  +T DE Q+Q + 
Sbjct:  605 ELHTLRRICLFTITIYVKAWFTAPSSCEAPCNDLCMLQSLELFAAVDSQVAGIAIKKMRSHLWYLSEDLIGLALFSVDVWDSEKTAMVDALKKSKMQTDLRRVDPKTITEFQTKTLPDFVTKRSLNLFTAMHIDP--AFLTGQPSTWSECPGYISGKQKITSLKVINDCAKRAVKLETDFNNILTHDESQRQLVF 797

HSP 2 Score: 36.965 bits (84), Expect = 7.140e-22
Identity = 17/37 (45.95%), Postives = 25/37 (67.57%), Query Frame = 3 
Query:  336 EILRKEMVAFYTNAITYR-QPREDYLELLRLCLVFLG 443
            ++ R E+V+F  N I+   QPR+DYLE  +L L+ LG
Sbjct:  551 DVKRAEVVSFLENLISKEMQPRQDYLEFCKLSLIMLG 587
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BLAST of NADH-quinone oxidoreductase subunit D vs. Planmine SMEST
Match: SMESG000054857.1 (SMESG000054857.1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.469e-19
Identity = 57/166 (34.34%), Postives = 83/166 (50.00%), Query Frame = 1 
Query:  478 MAKAIYSLKMVLFQGQMTLTARELKGLTELALFVALVYGRFWHEAPLASHAHLNDA*MLRLLQTYP--N*TVADAAFSAL-CHLWFFSEHLVGLAFFDDRVESHVKKAMVANLQLPKTRSALHRVDSSDADFNNLETFVTEITNRLFELVSATGVEEFRSFLLKEP 966
            M K IY LK+ LF+ Q  LT +EL+ L  + LF   +Y + W  AP +  A  ND  ML+ L+ +   +  VAD A   +  H+W  S+ L+GLA F D V    K AMV  L+  K ++ L RVD         +T    +T R   L +   ++   +FL  +P
Sbjct:    1 MTKGIYCLKIYLFREQFKLTQQELQALRCICLFTITIYVKAWFTAPSSCDAPCNDLCMLQSLELFAAVDSQVADIAIKKMRGHMWQLSDDLIGLALFSDYVWESEKTAMVDALKKSKMQTDLRRVDPKTITAFQRKTLSDFVTERSLNLFTTMHIDP--AFLTGQP 164
[Close]
BLAST of NADH-quinone oxidoreductase subunit D vs. Planmine SMEST
Match: SMESG000043035.1 (SMESG000043035.1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.624e-17
Identity = 48/145 (33.10%), Postives = 73/145 (50.34%), Query Frame = 1 
Query:  685 VADAAFSAL-CHLWFFSEHLVGLAFFDDRVESHVKKAMVANLQLPKTRSALHRVDSSDADFNNLETFVTEITNRLFELVSATGVEEFRSFLLKEPEA*EADASYQKLYERVKMI*VVNDSAERGIALIEKYNESITEDEDQKQFL 1116
            VAD A   +  HLW+ SE L+GLA F D V    K+AMV  L+ PK ++ L RVD         +T    +T R   L +A  ++   +FL  +P        Y    +++  + V+ND A+R + L   +N  +T DE Q+Q +
Sbjct:   23 VADIAIKKMRGHLWYLSEDLIGLALFSDDVWDSEKRAMVDALKKPKMQTDLRRVDPKTITAFQTKTLSDFVTERSLNLFTAMRID--LAFLTGQPSTWSECPGYISGKQKITSLRVINDCAKRAVKLATDFNNMLTHDESQRQLV 165
The following BLAST results are available for this feature:
BLAST of NADH-quinone oxidoreductase subunit D vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NADH-quinone oxidoreductase subunit D vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NADH-quinone oxidoreductase subunit D vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NADH-quinone oxidoreductase subunit D vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NADH-quinone oxidoreductase subunit D vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NADH-quinone oxidoreductase subunit D vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NADH-quinone oxidoreductase subunit D vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NADH-quinone oxidoreductase subunit D vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A151ICQ41.532e-6037.39Uncharacterized protein OS=Cyphomyrmex costatus OX... [more]
A0A151IT707.324e-5935.81Uncharacterized protein (Fragment) OS=Trachymyrmex... [more]
A0A151IV028.458e-5835.37Uncharacterized protein OS=Trachymyrmex cornetzi O... [more]
J9LU942.302e-5639.47Uncharacterized protein OS=Acyrthosiphon pisum OX=... [more]
A0A2S2PCY91.040e-5539.04Uncharacterized protein OS=Schizaphis graminum OX=... [more]
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BLAST of NADH-quinone oxidoreductase subunit D vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NADH-quinone oxidoreductase subunit D vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NADH-quinone oxidoreductase subunit D vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NADH-quinone oxidoreductase subunit D vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NADH-quinone oxidoreductase subunit D vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NADH-quinone oxidoreductase subunit D vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000028508.15.243e-3533.56SMESG000028508.1[more]
SMESG000067123.18.268e-2436.36SMESG000067123.1[more]
SMESG000006554.17.140e-2230.77SMESG000006554.1[more]
SMESG000054857.11.469e-1934.34SMESG000054857.1[more]
SMESG000043035.11.624e-1733.10SMESG000043035.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012038 ID=SMED30012038|Name=NADH-quinone oxidoreductase subunit D|organism=Schmidtea mediterranea sexual|type=transcript|length=1121bp
TTAACACTGTTATACACTTATCCGGATGCACTGGGCATATAACATGGAAA
CTACGCGATGTAGTGCAGGGTCTGGTGTTTGATACCACATCGTCCAACAC
TGGCATTCACAAGGGAGCTTGTGTCCTCATCGAGTAAGCCCTTGTTCGTG
AGCTTGTCAATATCGGCTGTTATCATCACGTCTTGGAAGTCGTCCTTAGC
AGCGTATTCACAGTTTTGTTTGGTGGAACTGGAGGGCCTGAAGTAGGCCT
GTTATAGAGGTTTAAGAATAAATGGTCATATATTAAACAAAACGATTACT
CGCATGCTAAAAATGAACTCTTCATTAGTGTTACTGAAATCCTCCGGAAA
GAAATGGTGGCATTCTACACTAATGCCATTACTTATCGTCAGCCAAGAGA
GGATTACCTCGAACTCCTTCGATTGTGCTTGGTCTTCCTTGGATGAGGCT
CCTGGAGCAATGTACCACGCAAAGCGGATGGCCAAGGCCATCTATTCTCT
GAAGATGGTGCTGTTTCAAGGCCAAATGACTTTGACTGCCAGAGAGCTTA
AAGGACTGACTGAACTTGCTCTCTTCGTCGCTCTTGTTTATGGTCGCTTC
TGGCATGAGGCTCCATTGGCCTCTCATGCTCATTTGAATGATGCATAGAT
GCTTAGACTTCTTCAAACATATCCAAACTGAACCGTTGCAGATGCTGCAT
TTTCTGCCCTTTGTCATCTTTGGTTCTTCTCGGAACACCTTGTGGGTCTG
GCATTTTTCGACGATCGAGTAGAGTCACATGTGAAGAAGGCCATGGTAGC
CAACCTCCAACTTCCTAAGACTCGTTCAGCACTGCATAGGGTGGACTCCA
GTGATGCTGACTTCAACAATTTGGAGACATTCGTGACCGAGATAACCAAT
CGTCTCTTCGAACTGGTTAGCGCAACAGGGGTAGAGGAGTTCAGGAGCTT
CTTGTTGAAGGAACCAGAAGCATGAGAGGCTGATGCATCTTACCAGAAGT
TGTATGAGAGAGTCAAAATGATTTAAGTGGTTAATGACAGCGCAGAACGT
GGGATCGCTCTCATCGAAAAATACAACGAGTCTATCACGGAGGACGAAGA
TCAAAAGCAGTTCCTGCTTCG
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protein sequence of SMED30012038-orf-1

>SMED30012038-orf-1 ID=SMED30012038-orf-1|Name=SMED30012038-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=115bp
MLKMNSSLVLLKSSGKKWWHSTLMPLLIVSQERITSNSFDCAWSSLDEAP
GAMYHAKRMAKAIYSLKMVLFQGQMTLTARELKGLTELALFVALVYGRFW
HEAPLASHAHLNDA*
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protein sequence of SMED30012038-orf-2

>SMED30012038-orf-2 ID=SMED30012038-orf-2|Name=SMED30012038-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=113bp
MTKGRKCSICNGSVWICLKKSKHLCIIQMSMRGQWSLMPEATINKSDEES
KFSQSFKLSGSQSHLALKQHHLQRIDGLGHPLCVVHCSRSLIQGRPSTIE
GVRGNPLLADDK*
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