SMED30012030

Overview
NameSMED30012030
Smed IDSMED30012030
Length (bp)754
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30012030 (SMED30012030) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30012030 (SMED30012030) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30012030 (SMED30012030) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30012030 (SMED30012030) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012030

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
posterior sideSMED30012030SMESG000065864.1 dd_Smed_v4_5032_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30012030SMESG000065864.1 dd_Smed_v4_5032_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30012030 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001668:58964..59835 +3522
v31.017957:9866..10737 +3486
Homology
[Close]
BLAST of SMED30012030 vs. Ensembl Human
Match: UBL4A (ubiquitin like 4A [Source:HGNC Symbol;Acc:HGNC:12505])

HSP 1 Score: 63.929 bits (154), Expect = 3.488e-12
Identity = 41/150 (27.33%), Postives = 81/150 (54.00%), Query Frame = 2 
Query:   11 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPVLTNGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLA-RLWFPE 457
            M + +K LQG EC +++ E+  VS L++LV EKL +    ++++F  KGK++   + K + DY+I    K+NL++KP+     +       + S   +  + + + L  H++      +++Q  +  E  ++++   D+E+LA R   PE
Sbjct:    1 MQLTVKALQGRECSLQVPEDELVSTLKQLVSEKLNVPVRQQRLLF--KGKALA--DGKRLSDYSIGPNSKLNLVVKPLEKVLLEEGEAQRLADSPPPQVWQLISKVLARHFSAADASRVLEQLQRDYERSLSRLTLDDIERLASRFLHPE 146
[Close]
BLAST of SMED30012030 vs. Ensembl Human
Match: UBL4A (ubiquitin like 4A [Source:HGNC Symbol;Acc:HGNC:12505])

HSP 1 Score: 60.8474 bits (146), Expect = 8.262e-11
Identity = 37/143 (25.87%), Postives = 77/143 (53.85%), Query Frame = 2 
Query:   11 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPVLTNGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLA 439
            M + +K LQG EC +++ E+  VS L++LV EKL +    ++++F  KGK++   + K + DY+I    K+NL++KP+     +       + S   +  + + + L  H++      +++Q  +  E  ++++   D+E+ A
Sbjct:    1 MQLTVKALQGRECSLQVPEDELVSTLKQLVSEKLNVPVRQQRLLF--KGKALA--DGKRLSDYSIGPNSKLNLVVKPLEKVLLEEGEAQRLADSPPPQVWQLISKVLARHFSAADASRVLEQLQRDYERSLSRLTLDDIERWA 139
[Close]
BLAST of SMED30012030 vs. Ensembl Human
Match: UBL4B (ubiquitin like 4B [Source:HGNC Symbol;Acc:HGNC:32309])

HSP 1 Score: 46.595 bits (109), Expect = 7.445e-6
Identity = 33/149 (22.15%), Postives = 66/149 (44.30%), Query Frame = 2 
Query:   11 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPVLTNGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLARLWFPE 457
            M + +K L G  C +++     V+ L+ LV  +L +    + ++F    +    +++K + DY I     +N++++P+     K      T          QL   L  H+  +    +++   Q  E+R+ K++   LEQLA+    E
Sbjct:    1 MFLTVKLLLGQRCSLKVSGQESVATLKRLVSRRLKVPEEQQHLLF----RGQLLEDDKHLSDYCIGPNASINVIMQPLEKMALKEAHQPQTQ-----PLWHQLGLVLAKHFEPQDAKAVLQLLRQEHEERLQKISLEHLEQLAQYLLAE 140
[Close]
BLAST of SMED30012030 vs. Ensembl Zebrafish
Match: UBL4A (zgc:56596 [Source:NCBI gene;Acc:393270])

HSP 1 Score: 75.8702 bits (185), Expect = 1.150e-16
Identity = 49/152 (32.24%), Postives = 87/152 (57.24%), Query Frame = 2 
Query:   11 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPV--LTNGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLA-RLWFPE 457
            MI+ +KPLQG EC +++ EN  VS ++ELV E+L I    +++  +YKGK++  DE++ + DY+I    K+NL+++P    ++G   T + N     S  + + L   L  H++      + +Q  +  E  + +++  D+E+LA RL  PE
Sbjct:    1 MILTVKPLQGKECNVQVTENEKVSTVKELVSERLNIPASQQRL--LYKGKAL-ADEHR-LSDYSIGPEAKLNLVVRPAGERSSGAVGTSSANNDKGGSGVW-QLLSTVLAKHFSPADAAKVQEQLIKDYERSLRQLSLDDIERLASRLLHPE 147
[Close]
BLAST of SMED30012030 vs. Ensembl Xenopus
Match: bend7 (BEN domain containing 7 [Source:Xenbase;Acc:XB-GENE-6039598])

HSP 1 Score: 47.7506 bits (112), Expect = 2.103e-6
Identity = 37/150 (24.67%), Postives = 77/150 (51.33%), Query Frame = 2 
Query:   11 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPVLTNGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLA-RLWFPE 457
            M + +K L+G E  I++ E   V  ++ LV+EKL +    ++++F  +GK++  DE+  +  Y+I  G ++NL++K  +     +  NT           K L   L  H++    + +++   +  E  ++ ++  D+E+LA R+  P+
Sbjct:    1 MQLTVKALKGKEANIQVSEGDTVLAVKRLVEEKLKVPVSQQRLLF--RGKALA-DEH-CLAHYSIGPGSRLNLMVKEQVAPEGHSGGNTAW---------KSLSVILRKHFSPTDAERVLEYVQKDYERSLSLLSLDDIERLATRILHPQ 137
[Close]
BLAST of SMED30012030 vs. Ensembl Mouse
Match: Ubl4a (ubiquitin-like 4A [Source:MGI Symbol;Acc:MGI:95049])

HSP 1 Score: 61.2326 bits (147), Expect = 2.595e-11
Identity = 40/150 (26.67%), Postives = 79/150 (52.67%), Query Frame = 2 
Query:   11 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPVLTNGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLA-RLWFPE 457
            M + +K LQG EC +++ E+  VS L+ LV +KL +    ++++F  KGK++   + K + DYNI    K+NL++KP+     +         S +    + + + L  H++      +++Q  +  +  ++++   D+E+LA R   PE
Sbjct:    1 MQLTVKALQGRECSLQVAEDELVSTLKHLVSDKLNVPVRQQRLLF--KGKALA--DEKRLSDYNIGPNSKLNLVVKPLEKVLLEEGSAHRLVDSPATPIWQLISKVLARHFSVADASRVLEQLQRDYDRSLSRLTLDDIERLASRFLHPE 146
[Close]
BLAST of SMED30012030 vs. Ensembl Mouse
Match: Ubl4a (ubiquitin-like 4A [Source:MGI Symbol;Acc:MGI:95049])

HSP 1 Score: 61.2326 bits (147), Expect = 2.595e-11
Identity = 40/150 (26.67%), Postives = 79/150 (52.67%), Query Frame = 2 
Query:   11 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPVLTNGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLA-RLWFPE 457
            M + +K LQG EC +++ E+  VS L+ LV +KL +    ++++F  KGK++   + K + DYNI    K+NL++KP+     +         S +    + + + L  H++      +++Q  +  +  ++++   D+E+LA R   PE
Sbjct:    1 MQLTVKALQGRECSLQVAEDELVSTLKHLVSDKLNVPVRQQRLLF--KGKALA--DEKRLSDYNIGPNSKLNLVVKPLEKVLLEEGSAHRLVDSPATPIWQLISKVLARHFSVADASRVLEQLQRDYDRSLSRLTLDDIERLASRFLHPE 146
[Close]
BLAST of SMED30012030 vs. UniProt/SwissProt
Match: sp|Q7ZWB2|UBL4A_DANRE (Ubiquitin-like protein 4A OS=Danio rerio OX=7955 GN=ubl4a PE=2 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 8.680e-16
Identity = 49/152 (32.24%), Postives = 87/152 (57.24%), Query Frame = 2 
Query:   11 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPV--LTNGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLA-RLWFPE 457
            MI+ +KPLQG EC +++ EN  VS ++ELV E+L I    +++  +YKGK++  DE++ + DY+I    K+NL+++P    ++G   T + N     S  + + L   L  H++      + +Q  +  E  + +++  D+E+LA RL  PE
Sbjct:    1 MILTVKPLQGKECNVQVTENEKVSTVKELVSERLNIPASQQRL--LYKGKAL-ADEHR-LSDYSIGPEAKLNLVVRPAGERSSGAVGTSSANNDKGGSGVW-QLLSTVLAKHFSPADAAKVQEQLIKDYERSLRQLSLDDIERLASRLLHPE 147
[Close]
BLAST of SMED30012030 vs. UniProt/SwissProt
Match: sp|C1BGZ8|UB4AA_ONCMY (Ubiquitin-like protein 4A-A OS=Oncorhynchus mykiss OX=8022 GN=ubl4aa PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 6.983e-14
Identity = 45/150 (30.00%), Postives = 82/150 (54.67%), Query Frame = 2 
Query:   11 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPVLTNGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLA-RLWFPE 457
            MI+ +KPLQG EC +++ E+  VS ++ELV E+L I    +++  +YKGK++  DE++ + DY+I    K+NL+++P             +S S      + L   L  H++      + +Q  +  E  + +++  D+E+LA RL  P+
Sbjct:    1 MILTVKPLQGKECNVQVTEDEKVSTVKELVSERLNIPASQQRL--LYKGKAL-ADEHR-LSDYSIGPEAKLNLVVRP--AGERSGVAGMASSSSAVGGVWQTLSTVLAKHFSPADAAKVQEQLVKDYERSLRQLSMDDIERLAGRLLHPD 144
[Close]
BLAST of SMED30012030 vs. UniProt/SwissProt
Match: sp|C3KHF2|UBL4A_ANOFI (Ubiquitin-like protein 4A OS=Anoplopoma fimbria OX=229290 GN=ubl4a PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 9.622e-14
Identity = 46/150 (30.67%), Postives = 87/150 (58.00%), Query Frame = 2 
Query:   11 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPVLTNGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLA-RLWFPE 457
            MI+ +KPLQG EC +++ E+  VS ++ELV E+L I    +++  +YKGK++ +DE++ + DY+I    K+NL+++PV      +    ++S S   R  + +   L  H++      + +Q  +  E  + +++  D+E+LA RL  P+
Sbjct:    1 MILTVKPLQGKECSVQVTEDEKVSTVKELVSERLNIPANQQRL--LYKGKAL-SDEHR-LSDYSIGPEAKLNLVIRPVGERTGASGTAASSSSSSQGRVWQTVSTILARHFSPADAAKVHEQLIKDYERSLRQLSLDDIERLAGRLLHPD 146
[Close]
BLAST of SMED30012030 vs. UniProt/SwissProt
Match: sp|C1BXU5|UBL4A_ESOLU (Ubiquitin-like protein 4A OS=Esox lucius OX=8010 GN=ubl4a PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.011e-13
Identity = 45/151 (29.80%), Postives = 87/151 (57.62%), Query Frame = 2 
Query:   11 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPVL-TNGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLA-RLWFPE 457
            MI+ +KPLQG EC +++ E+  VS ++ELV E+L I    +++  +YKGK++  DE++ + DY+I    K+NL+++P    +G     ++N+++       + L   L  H++      + +Q  +  E  + +++  D+E+LA RL  P+
Sbjct:    1 MILTVKPLQGKECNVQVTEDEKVSTVKELVSERLNIPPNQQRL--LYKGKAL-ADEHR-LSDYSIGPEAKLNLVVRPAGERSGVTGMASSNSAVGG---VWQTLSTVLAKHFSPADAAKVQEQLIKDYERSLRQLSLDDIERLAVRLLHPD 144
[Close]
BLAST of SMED30012030 vs. UniProt/SwissProt
Match: sp|B1MTV8|UBL4A_PLEMO (Ubiquitin-like protein 4A OS=Plecturocebus moloch OX=9523 GN=UBL4A PE=3 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 4.760e-12
Identity = 40/150 (26.67%), Postives = 81/150 (54.00%), Query Frame = 2 
Query:   11 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPVLTNGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLA-RLWFPE 457
            M + +K LQG EC +++ E+  VS L++LV EKL +    ++++F  KGK++   + K + DY+I    K+NL++KP+     +       + S   +  + + + L  H++      +++Q  +  +  ++++   D+E+LA R   PE
Sbjct:    1 MQLTVKALQGRECSLQVPEDELVSTLKQLVSEKLNVPVRQQRLLF--KGKALA--DGKRLSDYSIGPNSKLNLVVKPLEKVLLEEGAGRRLADSPPTQVWQLISKVLARHFSAADASRVLEQLQRDYQRSLSRLTLDDIERLASRFLHPE 146
[Close]
BLAST of SMED30012030 vs. TrEMBL
Match: A0A075A7K1 (Ubiquitin-like domain-containing protein OS=Opisthorchis viverrini OX=6198 GN=T265_08665 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 6.302e-14
Identity = 44/147 (29.93%), Postives = 83/147 (56.46%), Query Frame = 2 
Query:   11 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPV-LTNGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLARLW 448
            M ++IKPL   E  +E+  N+  S+L E V     +D   +++VF  KG  +  D+++++  Y I +G+K+ L++K V + + F+          +   F+  L+ +L   Y+D ++++IV +F   L  RIN M+ +D+E+LA +W
Sbjct:    1 MKLRIKPLTKPEQSVEVSSNATTSDLFEAVHSAFGVDPAGQRLVF--KGCPLL-DKSRLVSQYGIRDGDKLTLVIKQVSVPDSFQE---------RRPSFETLLRNHLAHTYSDREVEVIVSKFMHILFTRINAMSLNDIERLAEIW 135
[Close]
BLAST of SMED30012030 vs. TrEMBL
Match: A0A5J4NFY7 (Ubiquitin-like domain-containing protein OS=Paragonimus westermani OX=34504 GN=DEA37_0012127 PE=4 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 7.679e-14
Identity = 46/147 (31.29%), Postives = 82/147 (55.78%), Query Frame = 2 
Query:   11 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPVLTNGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLARLWF 451
            MI+K+KPL   E  +++  ++ VSEL E+V+    +D+  +++VF  KG  +  D+ K + +Y I +G+K+ L+LK           +T T   +   F+  L+  L   Y +E++D IV +F   L  R++ M+  D+E+LA +W 
Sbjct:    1 MILKVKPLSRPEKSVQVLNSATVSELLEVVQNSFNVDSACQRLVF--KGCPLL-DKLKTLDEYGIKDGDKLTLILK-----------STPTKQVQQPNFESLLRARLSTVYTEEEVDSIVSKFMHILSARVSSMSLDDVERLAEIWL 133
[Close]
BLAST of SMED30012030 vs. TrEMBL
Match: A0A1S8WNR7 (Ubiquitin-like domain-containing protein OS=Opisthorchis viverrini OX=6198 GN=X801_08050 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 2.161e-13
Identity = 42/157 (26.75%), Postives = 83/157 (52.87%), Query Frame = 2 
Query:   11 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVF----------VYKGKSMTTDENKIIKDYNILNGEKVNLLLKPV-LTNGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLARLW 448
            M ++IKPL   E  +E+  N+  S+L E V     +D   +++VF           +   ++  D+++++  Y I +G+K+ L++K V + + F+          +   F+  L+ +L   Y+D ++++IV +F   L  RIN M+ +D+E+LA +W
Sbjct:    1 MKLRIKPLTKPEQSVEVSSNATTSDLFEAVHSAFGVDPAGQRLVFKGCPLLGTLFSHSSPTINLDKSRLVSQYGIRDGDKLTLVIKQVSVPDSFQE---------RRPSFETLLRNHLAHTYSDREVEVIVSKFMHILFTRINAMSLNDIERLAEIW 148
[Close]
BLAST of SMED30012030 vs. TrEMBL
Match: A0A5A9NWN6 (Ubiquitin-like protein 4A OS=Triplophysa tibetana OX=1572043 GN=E1301_Tti013386 PE=4 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 5.484e-13
Identity = 48/152 (31.58%), Postives = 86/152 (56.58%), Query Frame = 2 
Query:   11 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPVL--TNGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLA-RLWFPE 457
            MI+ +KPLQG EC +++ EN  VS ++ELV E+L I    +++  +YKGK++  DE++ + DY+I    K+NL+++P    T G   + ++  +   S+   + L   L  H+       + +Q  +  E  + +++  D+E+LA RL  PE
Sbjct:    1 MIVTVKPLQGKECNVQVTENEKVSTVKELVSERLNIPPSQQRL--LYKGKAL-ADEHR-LSDYSIGPEAKLNLVVRPPAERTGGAMGSSSSRNNEKTSSGVWQLLSTVLAKHFTPADAAKVQEQLIKDYERSLRQLSLDDIERLASRLLHPE 148
[Close]
BLAST of SMED30012030 vs. TrEMBL
Match: A0A3P8W958 (Ubiquitin like 4A OS=Cynoglossus semilaevis OX=244447 GN=UBL4A PE=4 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 6.122e-13
Identity = 45/150 (30.00%), Postives = 83/150 (55.33%), Query Frame = 2 
Query:   11 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPVLTNGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLA-RLWFPE 457
            MII +KPLQG EC +++ E+  V+ ++ELV E+L I    ++++F  KGK++  DE+  + DY+I    K+NL+++P   +G      + +S +      + L   L  H+       + +Q  +  E  + +++  D+E+LA RL  P+
Sbjct:    1 MIITVKPLQGKECNVQVTEDEKVARVKELVSERLNIPANQQRLLF--KGKAL-ADEHS-LSDYSIGPESKLNLVIRPAGGSGTSGLATSPSSSTAQGGVWQILSTILARHFTPADAAKVHEQLIKDYERSLRQLSLDDIERLAGRLLHPD 146
[Close]
BLAST of SMED30012030 vs. Ensembl Cavefish
Match: zgc:56596 (ubiquitin-like protein 4A-A [Source:NCBI gene;Acc:103037541])

HSP 1 Score: 59.3066 bits (142), Expect = 1.001e-10
Identity = 48/154 (31.17%), Postives = 84/154 (54.55%), Query Frame = 2 
Query:   11 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPVLTNGFKNTCNTNTSLSKSARFDKQ----LKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLA-RLWFPE 457
            MI+ +KPLQG EC +++ EN  VS ++ELV E+L I    +++  +YKGK++  DE + + DY+I    K+NL+++P          ++++S S S          L   L  H++      + +Q  +  E  + +++  D+E+LA RL  PE
Sbjct:    1 MILTVKPLQGKECNVQVTENEKVSTVKELVFERLNIPPNQQRL--LYKGKAL-ADEYR-LSDYSIGPEAKLNLVVRPAGERSSGAAVSSSSSSSSSTDGSSGVWQILSTVLARHFSPADAAKVQEQLIKDYERSLRQLSLDDIERLAGRLLHPE 150
[Close]
BLAST of SMED30012030 vs. Ensembl Nematostella
Match: EDO46866 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNI9])

HSP 1 Score: 55.4546 bits (132), Expect = 7.030e-10
Identity = 42/153 (27.45%), Postives = 82/153 (53.59%), Query Frame = 2 
Query:    8 KMIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPVLT---NGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLARLWFPE 457
            K+I+KI  L G E E+E+ E  P+  ++ LV ++L  D  +++   VYKGK++  D +  + +Y I +G K+ L +K + +   +  KN   +N        F  QL + L  H N++  D ++++  Q  +    +++  D+E++++L   E
Sbjct:    2 KVIVKI--LNGKEAELEVSETEPILAVKTLVAQEL--DVQVERQRLVYKGKTLADDCS--LDEYLIGDGSKLYLSIKKLSSQPGSSQKNYAGSN--------FWNQLHKLLKSHLNEKDADKVLQRCKQDFKSWSRRLSLDDIERMSQLHLEE 140
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BLAST of SMED30012030 vs. Ensembl Medaka
Match: zgc:56596 (ubiquitin like 4A [Source:NCBI gene;Acc:101168961])

HSP 1 Score: 67.781 bits (164), Expect = 7.350e-14
Identity = 45/152 (29.61%), Postives = 85/152 (55.92%), Query Frame = 2 
Query:   11 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPVLTNGFKNTCNTNTSLSKS--ARFDKQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLA-RLWFPE 457
            MI+ +KPLQG EC +++ E+  VS ++ELV E+L I    +++  +YKGK++  DE++ + DY+I    K+NL+++P    G ++  +  T  S        + +   L  H++      + +Q  +  E  + +++  D+E+LA RL  P+
Sbjct:    1 MILTVKPLQGKECSVQVTEDEKVSTVKELVSEQLNIPANQQRL--LYKGKAL-ADEHR-LSDYSIGPEAKLNLVIRPA---GERSAASAVTGCSSRNMGGLWQTVSTILARHFSPADAAKVHEQLIKDYERSLRQLSLDDIERLAVRLLHPD 145
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BLAST of SMED30012030 vs. Planmine SMEST
Match: SMESG000065864.1 (SMESG000065864.1)

HSP 1 Score: 304.294 bits (778), Expect = 2.697e-106
Identity = 148/149 (99.33%), Postives = 149/149 (100.00%), Query Frame = 2 
Query:   11 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPVLTNGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLARLWFPE 457
            MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPVLTNGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLI+KQFHQTLEDRINKMNYSDLEQLARLWFPE
Sbjct:    1 MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGKSMTTDENKIIKDYNILNGEKVNLLLKPVLTNGFKNTCNTNTSLSKSARFDKQLKEYLFPHYNDEQIDLIIKQFHQTLEDRINKMNYSDLEQLARLWFPE 149
The following BLAST results are available for this feature:
BLAST of SMED30012030 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
UBL4A3.488e-1227.33ubiquitin like 4A [Source:HGNC Symbol;Acc:HGNC:125... [more]
UBL4A8.262e-1125.87ubiquitin like 4A [Source:HGNC Symbol;Acc:HGNC:125... [more]
UBL4B7.445e-622.15ubiquitin like 4B [Source:HGNC Symbol;Acc:HGNC:323... [more]
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BLAST of SMED30012030 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012030 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012030 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
UBL4A1.150e-1632.24zgc:56596 [Source:NCBI gene;Acc:393270][more]
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BLAST of SMED30012030 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
bend72.103e-624.67BEN domain containing 7 [Source:Xenbase;Acc:XB-GEN... [more]
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BLAST of SMED30012030 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Ubl4a2.595e-1126.67ubiquitin-like 4A [Source:MGI Symbol;Acc:MGI:95049... [more]
Ubl4a2.595e-1126.67ubiquitin-like 4A [Source:MGI Symbol;Acc:MGI:95049... [more]
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BLAST of SMED30012030 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q7ZWB2|UBL4A_DANRE8.680e-1632.24Ubiquitin-like protein 4A OS=Danio rerio OX=7955 G... [more]
sp|C1BGZ8|UB4AA_ONCMY6.983e-1430.00Ubiquitin-like protein 4A-A OS=Oncorhynchus mykiss... [more]
sp|C3KHF2|UBL4A_ANOFI9.622e-1430.67Ubiquitin-like protein 4A OS=Anoplopoma fimbria OX... [more]
sp|C1BXU5|UBL4A_ESOLU1.011e-1329.80Ubiquitin-like protein 4A OS=Esox lucius OX=8010 G... [more]
sp|B1MTV8|UBL4A_PLEMO4.760e-1226.67Ubiquitin-like protein 4A OS=Plecturocebus moloch ... [more]
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BLAST of SMED30012030 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A075A7K16.302e-1429.93Ubiquitin-like domain-containing protein OS=Opisth... [more]
A0A5J4NFY77.679e-1431.29Ubiquitin-like domain-containing protein OS=Parago... [more]
A0A1S8WNR72.161e-1326.75Ubiquitin-like domain-containing protein OS=Opisth... [more]
A0A5A9NWN65.484e-1331.58Ubiquitin-like protein 4A OS=Triplophysa tibetana ... [more]
A0A3P8W9586.122e-1330.00Ubiquitin like 4A OS=Cynoglossus semilaevis OX=244... [more]
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BLAST of SMED30012030 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:565961.001e-1031.17ubiquitin-like protein 4A-A [Source:NCBI gene;Acc:... [more]
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BLAST of SMED30012030 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012030 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30012030 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO468667.030e-1027.45Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30012030 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:565967.350e-1429.61ubiquitin like 4A [Source:NCBI gene;Acc:101168961][more]
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BLAST of SMED30012030 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000065864.12.697e-10699.33SMESG000065864.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012030 ID=SMED30012030|Name=SMED30012030|organism=Schmidtea mediterranea sexual|type=transcript|length=754bp
TTTGTGAAAGATGATTATTAAAATCAAACCTCTTCAGGGAGCTGAATGTG
AAATCGAAATAGATGAAAATTCTCCTGTAAGTGAACTTCAAGAGTTAGTT
AAAGAGAAATTATGTATAGATACCATGCTTAAAAAAATGGTCTTTGTATA
TAAAGGAAAATCAATGACAACGGATGAAAATAAAATTATTAAAGATTATA
ATATACTAAATGGAGAAAAGGTCAACCTATTATTAAAACCAGTTTTAACC
AATGGTTTTAAAAACACTTGCAATACAAACACATCATTATCGAAAAGTGC
AAGATTTGATAAACAATTAAAAGAATATTTATTTCCACATTATAATGATG
AACAAATTGATTTGATCGTAAAGCAGTTTCATCAAACTCTCGAAGATAGA
ATTAATAAGATGAATTATAGTGATTTGGAGCAACTAGCAAGACTTTGGTT
TCCTGAATAAAATTTCAATTTTTTAATTTAATTAAAATATGTAAAAATTT
GACTTTGAAATTTGATTTGATTTTGCGAGTTATTTTGTTCGATATTTTAC
CGTGTATTAGTTGTGGAATTTTCTGTATTTTAATATGAGTAGCCGTGATG
TAAGTGCGATTATTAGGTAATAATGCCAAATAATCACAAAAAAAAATTTT
ACTATCAGTCAATTTTATTGCTCGATTTCAGTTATTTACATAAAATCGAT
TTTCTGAAATTATGGATTTTAATTAGTGCGCGTAAGCGAAGAAATTTCTA
TTTG
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protein sequence of SMED30012030-orf-1

>SMED30012030-orf-1 ID=SMED30012030-orf-1|Name=SMED30012030-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=150bp
MIIKIKPLQGAECEIEIDENSPVSELQELVKEKLCIDTMLKKMVFVYKGK
SMTTDENKIIKDYNILNGEKVNLLLKPVLTNGFKNTCNTNTSLSKSARFD
KQLKEYLFPHYNDEQIDLIVKQFHQTLEDRINKMNYSDLEQLARLWFPE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR000626Ubiquitin-like_dom
IPR029071Ubiquitin-like_domsf
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0003674molecular_function
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
GO:0005829cytosol
GO:0071818BAT3 complex
Vocabulary: biological process
TermDefinition
GO:0006810transport
GO:0071816tail-anchored membrane protein insertion into ER membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.10.20.90coord: 1..78
e-value: 6.9E-10
score: 40.5
NoneNo IPR availablePANTHERPTHR46555FAMILY NOT NAMEDcoord: 1..146
NoneNo IPR availableCDDcd01807Ubl_UBL4A_likecoord: 1..76
e-value: 7.34159E-15
score: 63.1508
IPR000626Ubiquitin-like domainPFAMPF00240ubiquitincoord: 3..77
e-value: 7.7E-7
score: 28.8
IPR000626Ubiquitin-like domainPROSITEPS50053UBIQUITIN_2coord: 1..76
score: 10.898
IPR029071Ubiquitin-like domain superfamilySUPERFAMILYSSF54236Ubiquitin-likecoord: 1..76