e22/23 protein

Overview
Namee22/23 protein (AGZ94907.1)
Smed IDSMED30012010
Length (bp)503
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of e22/23 protein (SMED30012010) t-SNE clustered cells

Violin plots show distribution of expression levels for e22/23 protein (SMED30012010) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of e22/23 protein (SMED30012010) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for e22/23 protein (SMED30012010) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012010

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30012010h1SMcG0012768 dd_Smed_v4_12722_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30012010h1SMcG0012768 dd_Smed_v4_12722_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
central nervous systemSMED30012010h1SMcG0012768 KF487092smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30012010h1SMcG0012768 KF487092smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of e22/23 protein vs. Ensembl Human
Match: BHLHE23 (basic helix-loop-helix family member e23 [Source:HGNC Symbol;Acc:HGNC:16093])

HSP 1 Score: 123.635 bits (309), Expect = 3.487e-35
Identity = 63/98 (64.29%), Postives = 75/98 (76.53%), Query Frame = 2 
Query:   95 GQKYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQSRGYLSNI 388
            G   ++D R      + LRLSINARERRRMHDLNDALD LR+VIPYAHSPSVRKLSKIATLLLAKNYILMQA AL+E+R+ +  +N   Q +G  + +
Sbjct:   84 GPGSAADGRRRPREQRSLRLSINARERRRMHDLNDALDGLRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALDEMRRLVAFLN---QGQGLAAPV 178
[Close]
BLAST of e22/23 protein vs. Ensembl Human
Match: BHLHE23 (basic helix-loop-helix family member e23 [Source:HGNC Symbol;Acc:HGNC:16093])

HSP 1 Score: 124.02 bits (310), Expect = 4.584e-35
Identity = 63/98 (64.29%), Postives = 75/98 (76.53%), Query Frame = 2 
Query:   95 GQKYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQSRGYLSNI 388
            G   ++D R      + LRLSINARERRRMHDLNDALD LR+VIPYAHSPSVRKLSKIATLLLAKNYILMQA AL+E+R+ +  +N   Q +G  + +
Sbjct:  100 GPGSAADGRRRPREQRSLRLSINARERRRMHDLNDALDGLRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALDEMRRLVAFLN---QGQGLAAPV 194
[Close]
BLAST of e22/23 protein vs. Ensembl Human
Match: BHLHE22 (basic helix-loop-helix family member e22 [Source:HGNC Symbol;Acc:HGNC:11963])

HSP 1 Score: 126.716 bits (317), Expect = 7.945e-35
Identity = 60/71 (84.51%), Postives = 66/71 (92.96%), Query Frame = 2 
Query:  140 KPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMN 352
            K LRL+INARERRRMHDLNDALDELR+VIPYAHSPSVRKLSKIATLLLAKNYILMQA ALEE+R+ +  +N
Sbjct:  241 KALRLNINARERRRMHDLNDALDELRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLN 311
[Close]
BLAST of e22/23 protein vs. Ensembl Human
Match: OLIG3 (oligodendrocyte transcription factor 3 [Source:HGNC Symbol;Acc:HGNC:18003])

HSP 1 Score: 103.99 bits (258), Expect = 4.206e-27
Identity = 53/119 (44.54%), Postives = 74/119 (62.18%), Query Frame = 2 
Query:   83 SKVNGQ--KYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQSRGYLSNIKYEQSTQKITSKELH 433
            +K  G+  KY   ++  +   + LRL IN RER+RMHDLN A+D LR V+PYAH PSVRKLSKIATLLLA+NYILM  S+LEE+++ +  +     S  +   + +        +  +H
Sbjct:   61 AKAAGESSKYKIKKQLSEQDLQQLRLKINGRERKRMHDLNLAMDGLREVMPYAHGPSVRKLSKIATLLLARNYILMLTSSLEEMKRLVGEIYGGHHSAFHCGTVGHSAGHPAHAANSVH 179
[Close]
BLAST of e22/23 protein vs. Ensembl Human
Match: OLIG2 (oligodendrocyte transcription factor 2 [Source:HGNC Symbol;Acc:HGNC:9398])

HSP 1 Score: 101.293 bits (251), Expect = 9.738e-26
Identity = 46/65 (70.77%), Postives = 57/65 (87.69%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTI 340
            LRL IN+RER+RMHDLN A+D LR V+PYAH PSVRKLSKIATLLLA+NYILM  ++LEE+++ +
Sbjct:  109 LRLKINSRERKRMHDLNIAMDGLREVMPYAHGPSVRKLSKIATLLLARNYILMLTNSLEEMKRLV 173
[Close]
BLAST of e22/23 protein vs. Ensembl Celegans
Match: hlh-32 (Helix Loop Helix [Source:UniProtKB/TrEMBL;Acc:Q7YWS9])

HSP 1 Score: 106.686 bits (265), Expect = 1.950e-30
Identity = 59/103 (57.28%), Postives = 75/103 (72.82%), Query Frame = 2 
Query:   98 QKYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQSRGYLSNIKYEQST 406
            +K SSD  + +     +RLSIN RER RMHDLN+ALD+LR+VIPYAH  SVRKLSKIATLLLAKN+I+MQA A+EEL   +  + +R +S G   N++   ST
Sbjct:    2 EKMSSDLTFAEP---GVRLSINLRERCRMHDLNEALDDLRAVIPYAHGGSVRKLSKIATLLLAKNHIIMQAKAIEELSVLVSQLKTR-KSSGNSENLEKTVST 100
[Close]
BLAST of e22/23 protein vs. Ensembl Celegans
Match: hlh-17 (Helix Loop Helix [Source:UniProtKB/TrEMBL;Acc:O45489])

HSP 1 Score: 102.834 bits (255), Expect = 7.633e-29
Identity = 53/70 (75.71%), Postives = 62/70 (88.57%), Query Frame = 2 
Query:  125 IDSIF-KP-LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEEL 328
            ID+ F +P +RLSIN RER RMHDLN+ALD+LR+VIPYAH  SVRKLSKIATLLLAKN+I+MQA A+EEL
Sbjct:    6 IDTAFAEPGVRLSINLRERCRMHDLNEALDDLRAVIPYAHGGSVRKLSKIATLLLAKNHIIMQAKAIEEL 75
[Close]
BLAST of e22/23 protein vs. Ensembl Celegans
Match: hlh-31 (Helix Loop Helix [Source:UniProtKB/TrEMBL;Acc:Q86D08])

HSP 1 Score: 88.9669 bits (219), Expect = 9.451e-23
Identity = 41/53 (77.36%), Postives = 49/53 (92.45%), Query Frame = 2 
Query:  170 ERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEEL 328
            +R RMHDLN+ALD+LR+VIPYAH  SVRKLSKIATLLLAKN+I+M+A A+EEL
Sbjct:   83 KRCRMHDLNEALDDLRAVIPYAHGGSVRKLSKIATLLLAKNHIIMKAKAIEEL 135
[Close]
BLAST of e22/23 protein vs. Ensembl Celegans
Match: hlh-16 (Helix Loop Helix [Source:UniProtKB/TrEMBL;Acc:O45320])

HSP 1 Score: 70.4774 bits (171), Expect = 8.875e-16
Identity = 35/74 (47.30%), Postives = 50/74 (67.57%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQS 367
            LR SIN+RERRRMH+LND  + LR  +PY +  + R++SK  TLLLA N+I   A+A  +L+  + M N++  S
Sbjct:   41 LRNSINSRERRRMHELNDEFETLRECLPYPNEANSRRMSKANTLLLASNWIKQLANANHKLQMELNMANAKIDS 114
[Close]
BLAST of e22/23 protein vs. Ensembl Celegans
Match: cnd-1 (Neurogenic differentiation factor 1 [Source:UniProtKB/Swiss-Prot;Acc:P46581])

HSP 1 Score: 53.1434 bits (126), Expect = 4.946e-9
Identity = 29/50 (58.00%), Postives = 34/50 (68.00%), Query Frame = 2 
Query:  149 RLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYI 298
            R+  N RER RMH LN+ALD LR  IP   +   +KLSKI TL LA+NYI
Sbjct:   21 RVKANGRERARMHGLNNALDMLREYIPI--TTQHQKLSKIETLRLARNYI 68
[Close]
BLAST of e22/23 protein vs. Ensembl Fly
Match: Oli (gene:FBgn0032651 transcript:FBtr0080986)

HSP 1 Score: 124.02 bits (310), Expect = 1.558e-35
Identity = 59/70 (84.29%), Postives = 65/70 (92.86%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNS 355
            +RL+INARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQ +ALEELR+ +  + S
Sbjct:  129 VRLNINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQQNALEELRRLLAYIQS 198
[Close]
BLAST of e22/23 protein vs. Ensembl Fly
Match: Oli (gene:FBgn0032651 transcript:FBtr0344781)

HSP 1 Score: 124.02 bits (310), Expect = 1.558e-35
Identity = 59/70 (84.29%), Postives = 65/70 (92.86%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNS 355
            +RL+INARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQ +ALEELR+ +  + S
Sbjct:  129 VRLNINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQQNALEELRRLLAYIQS 198
[Close]
BLAST of e22/23 protein vs. Ensembl Fly
Match: Oli (gene:FBgn0032651 transcript:FBtr0303253)

HSP 1 Score: 124.02 bits (310), Expect = 1.558e-35
Identity = 59/70 (84.29%), Postives = 65/70 (92.86%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNS 355
            +RL+INARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQ +ALEELR+ +  + S
Sbjct:  129 VRLNINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQQNALEELRRLLAYIQS 198
[Close]
BLAST of e22/23 protein vs. Ensembl Fly
Match: Oli (gene:FBgn0032651 transcript:FBtr0303252)

HSP 1 Score: 124.02 bits (310), Expect = 1.558e-35
Identity = 59/70 (84.29%), Postives = 65/70 (92.86%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNS 355
            +RL+INARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQ +ALEELR+ +  + S
Sbjct:  129 VRLNINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQQNALEELRRLLAYIQS 198
[Close]
BLAST of e22/23 protein vs. Ensembl Fly
Match: dimm (gene:FBgn0023091 transcript:FBtr0333354)

HSP 1 Score: 59.3066 bits (142), Expect = 1.172e-10
Identity = 32/51 (62.75%), Postives = 34/51 (66.67%), Query Frame = 2 
Query:  149 RLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYIL 301
            RL  N RER RMH LNDA   LR VIP  H    R+LSKI TL LAKNYI+
Sbjct:  158 RLESNERERMRMHSLNDAFQSLREVIP--HVEMERRLSKIETLTLAKNYII 206
[Close]
BLAST of e22/23 protein vs. Ensembl Zebrafish
Match: bhlhe22 (basic helix-loop-helix family, member e22 [Source:ZFIN;Acc:ZDB-GENE-040426-1411])

HSP 1 Score: 125.946 bits (315), Expect = 3.982e-36
Identity = 59/69 (85.51%), Postives = 65/69 (94.20%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMN 352
            LRL+INARERRRMHDLNDALDELR+VIPYAHSPSVRKLSKIATLLLAKNYILMQA ALEE+R+ +  +N
Sbjct:  108 LRLNINARERRRMHDLNDALDELRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLN 176
[Close]
BLAST of e22/23 protein vs. Ensembl Zebrafish
Match: bhlhe23 (basic helix-loop-helix family, member e23 [Source:ZFIN;Acc:ZDB-GENE-050913-22])

HSP 1 Score: 125.561 bits (314), Expect = 5.272e-36
Identity = 60/69 (86.96%), Postives = 64/69 (92.75%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMN 352
            LRLSINARERRRMHDLNDALD LRSVIPYAHSPSVRKLSKIATLLLAKNYILMQA ALEE+R+ +  +N
Sbjct:  105 LRLSINARERRRMHDLNDALDGLRSVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLN 173
[Close]
BLAST of e22/23 protein vs. Ensembl Zebrafish
Match: bhlhe22 (basic helix-loop-helix family, member e22 [Source:ZFIN;Acc:ZDB-GENE-040426-1411])

HSP 1 Score: 125.561 bits (314), Expect = 1.030e-35
Identity = 59/69 (85.51%), Postives = 65/69 (94.20%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMN 352
            LRL+INARERRRMHDLNDALDELR+VIPYAHSPSVRKLSKIATLLLAKNYILMQA ALEE+R+ +  +N
Sbjct:  131 LRLNINARERRRMHDLNDALDELRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLN 199
[Close]
BLAST of e22/23 protein vs. Ensembl Zebrafish
Match: olig3 (oligodendrocyte transcription factor 3 [Source:ZFIN;Acc:ZDB-GENE-080903-1])

HSP 1 Score: 106.686 bits (265), Expect = 1.893e-28
Identity = 51/114 (44.74%), Postives = 74/114 (64.91%), Query Frame = 2 
Query:   92 NGQKYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQSRGYLSNIKYEQSTQKITSKELH 433
            +G KY   ++  +   + LRL IN RER+RMHDLN A+D LR V+PYAH PSVRKLSKIATLLLA+NYILM  S+L+E+++ +  +     S  +   + +  +     + ++H
Sbjct:   57 SGSKYKLKKQVTEQEIQQLRLKINGRERKRMHDLNLAMDGLREVMPYAHGPSVRKLSKIATLLLARNYILMLTSSLDEMKRLVGEIYGGHHSAFHCGTVSHGGAHSAGAAHQVH 170
[Close]
BLAST of e22/23 protein vs. Ensembl Zebrafish
Match: si:ch211-196c10.13 (si:ch211-196c10.13 [Source:ZFIN;Acc:ZDB-GENE-130603-71])

HSP 1 Score: 102.449 bits (254), Expect = 1.425e-27
Identity = 57/108 (52.78%), Postives = 76/108 (70.37%), Query Frame = 2 
Query:   35 AVSQSESDSNPNTMMISKVNGQKYSSDRRYIDSIFKP--LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMN 352
              + +E+D      + S  +G   SS R    ++ +P  LRL INARERRRMHDLN+ALD+LR+VIPY  +  V+KLSKIATLLLAKN+ILMQA AL+E+R+ +  M+
Sbjct:   57 VTTSAETDRKSFQGLESGCHGNSVSSSR----TVKEPGALRLLINARERRRMHDLNEALDDLRAVIPYTSNRKVKKLSKIATLLLAKNHILMQARALKEMRRIVEQMD 160
[Close]
BLAST of e22/23 protein vs. Ensembl Xenopus
Match: lbr (lamin B receptor [Source:Xenbase;Acc:XB-GENE-979300])

HSP 1 Score: 125.176 bits (313), Expect = 4.531e-35
Identity = 63/88 (71.59%), Postives = 71/88 (80.68%), Query Frame = 2 
Query:   89 VNGQKYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMN 352
              G K S ++R        LRL+INARERRRMHDLNDALDELR+VIPYAHSPSVRKLSKIATLLLAKNYILMQA ALEE+R+ +  +N
Sbjct:  142 TGGGKKSKEQRT-------LRLNINARERRRMHDLNDALDELRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLN 222
[Close]
BLAST of e22/23 protein vs. Ensembl Xenopus
Match: zbed4 (zinc finger, BED-type containing 4 [Source:Xenbase;Acc:XB-GENE-6076969])

HSP 1 Score: 104.76 bits (260), Expect = 1.819e-27
Identity = 54/120 (45.00%), Postives = 74/120 (61.67%), Query Frame = 2 
Query:  101 KYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQSRGYLSNIKYEQSTQKITSKELHSDNVSVQKH 460
            KY   ++  +   + LRL IN RER+RMHDLN A+D LR V+PYAH PSVRKLSKIATLLLA+NYILM  S+LEE+++ +  +     S  +   + +        +  +H  N + Q H
Sbjct:   64 KYKIKKQLSEQDLQQLRLKINGRERKRMHDLNLAMDGLREVMPYAHGPSVRKLSKIATLLLARNYILMLTSSLEEMKRLVGEIYGGHHSAFHCGTVGHS------ANHPVHPPNAAHQVH 177
[Close]
BLAST of e22/23 protein vs. Ensembl Xenopus
Match: OLIG2 (oligodendrocyte transcription factor 2 [Source:HGNC Symbol;Acc:HGNC:9398])

HSP 1 Score: 101.293 bits (251), Expect = 7.077e-27
Identity = 51/102 (50.00%), Postives = 70/102 (68.63%), Query Frame = 2 
Query:  137 FKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTI--------MMMNSRFQSRGYLSNIKYEQSTQKIT 418
             + LRL IN+RER+RMHDLN A+D LR V+PYAH PSVRKLSKIATLLLA+NYILM  ++LEE+++ +        +++ SR +  G  S  +      +I+
Sbjct:   89 LQQLRLKINSRERKRMHDLNIAMDGLREVMPYAHGPSVRKLSKIATLLLARNYILMLNNSLEEMKRLVNMSGPGPRLLLGSRLERLGIWSRQELNSGLLRIS 190
[Close]
BLAST of e22/23 protein vs. Ensembl Xenopus
Match: gps1 (G protein pathway suppressor 1 [Source:Xenbase;Acc:XB-GENE-955148])

HSP 1 Score: 95.1301 bits (235), Expect = 2.248e-24
Identity = 41/65 (63.08%), Postives = 58/65 (89.23%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTI 340
            LRL +N+RER+RMHDLN A+D LR V+PY+H PSVRKLSKI+TL+LA+NYI+M +++LEE+++ +
Sbjct:   59 LRLKVNSRERQRMHDLNQAMDGLREVMPYSHGPSVRKLSKISTLILARNYIVMLSNSLEEMKRLV 123
[Close]
BLAST of e22/23 protein vs. Ensembl Xenopus
Match: znf423 (zinc finger protein 423 [Source:Xenbase;Acc:XB-GENE-490169])

HSP 1 Score: 59.3066 bits (142), Expect = 5.369e-11
Identity = 31/50 (62.00%), Postives = 35/50 (70.00%), Query Frame = 2 
Query:  149 RLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYI 298
            RL  N RER+RMH LN+A   LR VIP  H  + +KLSKI TL LAKNYI
Sbjct:   68 RLESNERERQRMHKLNNAFQALREVIP--HVRAEKKLSKIETLTLAKNYI 115
[Close]
BLAST of e22/23 protein vs. Ensembl Mouse
Match: Bhlhe23 (basic helix-loop-helix family, member e23 [Source:MGI Symbol;Acc:MGI:2153710])

HSP 1 Score: 125.561 bits (314), Expect = 5.022e-36
Identity = 59/69 (85.51%), Postives = 64/69 (92.75%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMN 352
            LRLSINARERRRMHDLNDALD LR+VIPYAHSPSVRKLSKIATLLLAKNYILMQA ALEE+R+ +  +N
Sbjct:   99 LRLSINARERRRMHDLNDALDGLRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLN 167
[Close]
BLAST of e22/23 protein vs. Ensembl Mouse
Match: Bhlhe22 (basic helix-loop-helix family, member e22 [Source:MGI Symbol;Acc:MGI:1930001])

HSP 1 Score: 127.102 bits (318), Expect = 2.109e-35
Identity = 60/71 (84.51%), Postives = 66/71 (92.96%), Query Frame = 2 
Query:  140 KPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMN 352
            K LRL+INARERRRMHDLNDALDELR+VIPYAHSPSVRKLSKIATLLLAKNYILMQA ALEE+R+ +  +N
Sbjct:  215 KALRLNINARERRRMHDLNDALDELRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLN 285
[Close]
BLAST of e22/23 protein vs. Ensembl Mouse
Match: Olig3 (oligodendrocyte transcription factor 3 [Source:MGI Symbol;Acc:MGI:2149955])

HSP 1 Score: 103.99 bits (258), Expect = 3.072e-27
Identity = 53/119 (44.54%), Postives = 74/119 (62.18%), Query Frame = 2 
Query:   83 SKVNGQ--KYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQSRGYLSNIKYEQSTQKITSKELH 433
            +K  G+  KY   ++  +   + LRL IN RER+RMHDLN A+D LR V+PYAH PSVRKLSKIATLLLA+NYILM  S+LEE+++ +  +     S  +   + +        +  +H
Sbjct:   62 AKAAGESSKYKIKKQLSEQDLQQLRLKINGRERKRMHDLNLAMDGLREVMPYAHGPSVRKLSKIATLLLARNYILMLTSSLEEMKRLVGEIYGGHHSAFHCGTVGHSAGHPAHAANAVH 180
[Close]
BLAST of e22/23 protein vs. Ensembl Mouse
Match: Olig2 (oligodendrocyte transcription factor 2 [Source:MGI Symbol;Acc:MGI:1355331])

HSP 1 Score: 100.908 bits (250), Expect = 1.069e-25
Identity = 46/65 (70.77%), Postives = 57/65 (87.69%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTI 340
            LRL IN+RER+RMHDLN A+D LR V+PYAH PSVRKLSKIATLLLA+NYILM  ++LEE+++ +
Sbjct:  109 LRLKINSRERKRMHDLNIAMDGLREVMPYAHGPSVRKLSKIATLLLARNYILMLTNSLEEMKRLV 173
[Close]
BLAST of e22/23 protein vs. Ensembl Mouse
Match: Olig1 (oligodendrocyte transcription factor 1 [Source:MGI Symbol;Acc:MGI:1355334])

HSP 1 Score: 75.0998 bits (183), Expect = 1.949e-16
Identity = 44/71 (61.97%), Postives = 54/71 (76.06%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVI-PY--AH---SPSVRKLSKIATLLLAKNYILMQASALEELRKTI 340
            LR  IN+RER+RM DLN A+D LR VI PY  AH   +P  RKLSKIATLLLA+NYIL+  S+L+ELR+ +
Sbjct:   95 LRRKINSRERKRMQDLNLAMDALREVILPYSAAHCQGAPG-RKLSKIATLLLARNYILLLGSSLQELRRAL 164
[Close]
BLAST of e22/23 protein vs. UniProt/SwissProt
Match: sp|Q8BGW3|BHE23_MOUSE (Class E basic helix-loop-helix protein 23 OS=Mus musculus OX=10090 GN=Bhlhe23 PE=2 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 3.513e-35
Identity = 59/69 (85.51%), Postives = 64/69 (92.75%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMN 352
            LRLSINARERRRMHDLNDALD LR+VIPYAHSPSVRKLSKIATLLLAKNYILMQA ALEE+R+ +  +N
Sbjct:   99 LRLSINARERRRMHDLNDALDGLRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLN 167
[Close]
BLAST of e22/23 protein vs. UniProt/SwissProt
Match: sp|Q71T09|BHE22_CHICK (Class E basic helix-loop-helix protein 22 OS=Gallus gallus OX=9031 GN=BHLHE22 PE=2 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 4.553e-35
Identity = 62/87 (71.26%), Postives = 72/87 (82.76%), Query Frame = 2 
Query:   92 NGQKYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMN 352
            NG  +   ++  +   K LRL+INARERRRMHDLNDALDELR+VIPYAHSPSVRKLSKIATLLLAKNYILMQA ALEE+R+ +  +N
Sbjct:  156 NGHGHGGSKKSKEQ--KALRLNINARERRRMHDLNDALDELRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLN 240
[Close]
BLAST of e22/23 protein vs. UniProt/SwissProt
Match: sp|O09029|BHE22_MESAU (Class E basic helix-loop-helix protein 22 OS=Mesocricetus auratus OX=10036 GN=BHLHE22 PE=2 SV=2)

HSP 1 Score: 127.102 bits (318), Expect = 1.574e-34
Identity = 60/71 (84.51%), Postives = 66/71 (92.96%), Query Frame = 2 
Query:  140 KPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMN 352
            K LRL+INARERRRMHDLNDALDELR+VIPYAHSPSVRKLSKIATLLLAKNYILMQA ALEE+R+ +  +N
Sbjct:  225 KALRLNINARERRRMHDLNDALDELRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLN 295
[Close]
BLAST of e22/23 protein vs. UniProt/SwissProt
Match: sp|Q8NDY6|BHE23_HUMAN (Class E basic helix-loop-helix protein 23 OS=Homo sapiens OX=9606 GN=BHLHE23 PE=1 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 1.675e-34
Identity = 63/98 (64.29%), Postives = 75/98 (76.53%), Query Frame = 2 
Query:   95 GQKYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQSRGYLSNI 388
            G   ++D R      + LRLSINARERRRMHDLNDALD LR+VIPYAHSPSVRKLSKIATLLLAKNYILMQA AL+E+R+ +  +N   Q +G  + +
Sbjct:   84 GPGSAADGRRRPREQRSLRLSINARERRRMHDLNDALDGLRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALDEMRRLVAFLN---QGQGLAAPV 178
[Close]
BLAST of e22/23 protein vs. UniProt/SwissProt
Match: sp|Q8C6A8|BHE22_MOUSE (Class E basic helix-loop-helix protein 22 OS=Mus musculus OX=10090 GN=Bhlhe22 PE=1 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 2.004e-34
Identity = 60/71 (84.51%), Postives = 66/71 (92.96%), Query Frame = 2 
Query:  140 KPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMN 352
            K LRL+INARERRRMHDLNDALDELR+VIPYAHSPSVRKLSKIATLLLAKNYILMQA ALEE+R+ +  +N
Sbjct:  215 KALRLNINARERRRMHDLNDALDELRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLN 285
[Close]
BLAST of e22/23 protein vs. TrEMBL
Match: U5YQ54 (E22/23 protein (Fragment) OS=Schmidtea mediterranea OX=79327 GN=23 PE=2 SV=1)

HSP 1 Score: 301.597 bits (771), Expect = 7.486e-103
Identity = 147/149 (98.66%), Postives = 148/149 (99.33%), Query Frame = 2 
Query:   17 DCCGSTAVSQSESDSNPNTMMISKVNGQKYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQSRGYLSNIKYEQSTQKITSKELHSDNVSVQKHL 463
            DCCGSTAVSQSESDSNPNTMMISKVN +KYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQSRGYLSNIKYEQSTQKITSKELHSDNVSVQKHL
Sbjct:    1 DCCGSTAVSQSESDSNPNTMMISKVNDKKYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQSRGYLSNIKYEQSTQKITSKELHSDNVSVQKHL 149
[Close]
BLAST of e22/23 protein vs. TrEMBL
Match: A0A0A7M2A7 (BETA3 transcription factor (Fragment) OS=Schmidtea polychroa OX=50054 GN=beta3 PE=2 SV=1)

HSP 1 Score: 290.426 bits (742), Expect = 1.060e-98
Identity = 142/145 (97.93%), Postives = 142/145 (97.93%), Query Frame = 2 
Query:   20 CCGSTAVSQSESDSNPNTMMISKVNGQKYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQSRGYLSNIKYEQSTQKITSKELHSDNVSVQ 454
              GSTAVSQSESDSNPNTMMISKVNGQKYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQSRGYLSNIKYE STQKITSKELHSDNVSVQ
Sbjct:    2 VAGSTAVSQSESDSNPNTMMISKVNGQKYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQSRGYLSNIKYEHSTQKITSKELHSDNVSVQ 146
[Close]
BLAST of e22/23 protein vs. TrEMBL
Match: A0A212FNL8 (BHLH domain-containing protein OS=Danaus plexippus plexippus OX=278856 GN=KGM_201350 PE=4 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 3.043e-35
Identity = 63/97 (64.95%), Postives = 74/97 (76.29%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQSRGYLSNIKYEQSTQKITSKELHS 436
            +RL+INARERRRMHDLNDALDELR VIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELR+ +  +     + G +    ++ +    T+K L  
Sbjct:   46 VRLNINARERRRMHDLNDALDELRGVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRRLVAYLQGTATAAGIVPPPGFDLAGMPATAKLLQG 142
[Close]
BLAST of e22/23 protein vs. TrEMBL
Match: E9HGJ9 (BHLH domain-containing protein (Fragment) OS=Daphnia pulex OX=6669 GN=DAPPUDRAFT_17020 PE=4 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 6.991e-35
Identity = 61/71 (85.92%), Postives = 67/71 (94.37%), Query Frame = 2 
Query:  140 KPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMN 352
            K +RLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYI+MQA+AL+ELR+ +  MN
Sbjct:    7 KAVRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYIMMQANALDELRRVVTYMN 77
[Close]
BLAST of e22/23 protein vs. TrEMBL
Match: A0A4Z2D0L4 (Class E basic helix-loop-helix protein OS=Schistosoma japonicum OX=6182 GN=EWB00_005801 PE=4 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 9.733e-35
Identity = 72/103 (69.90%), Postives = 80/103 (77.67%), Query Frame = 2 
Query:  149 RLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRF-QSRGYLSNIKYEQSTQKITSKEL----HSDN 442
            RLSINARERRRMHDLNDALD+LRSVIPYAHSPSVRKLSKIATLLLAKNYILMQA+A++EL K I+ +NS+  QS   L N  Y+  T K T  E     H DN
Sbjct:  104 RLSINARERRRMHDLNDALDDLRSVIPYAHSPSVRKLSKIATLLLAKNYILMQANAIDELYKLIICLNSQIQQSDSLLLNDDYKSITLKSTIPEFMHNTHCDN 206
[Close]
BLAST of e22/23 protein vs. Ensembl Cavefish
Match: bhlhe23 (basic helix-loop-helix family, member e23 [Source:ZFIN;Acc:ZDB-GENE-050913-22])

HSP 1 Score: 124.405 bits (311), Expect = 9.777e-36
Identity = 59/69 (85.51%), Postives = 64/69 (92.75%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMN 352
            LRLSINARERRRMHDLNDALD LR+VIPYAHSPSVRKLSKIATLLLAKNYILMQA ALEE+R+ +  +N
Sbjct:   94 LRLSINARERRRMHDLNDALDGLRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLN 162
[Close]
BLAST of e22/23 protein vs. Ensembl Cavefish
Match: bhlhe22 (basic helix-loop-helix family, member e22 [Source:ZFIN;Acc:ZDB-GENE-040426-1411])

HSP 1 Score: 125.176 bits (313), Expect = 1.184e-35
Identity = 59/69 (85.51%), Postives = 65/69 (94.20%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMN 352
            LRL+INARERRRMHDLNDALDELR+VIPYAHSPSVRKLSKIATLLLAKNYILMQA ALEE+R+ +  +N
Sbjct:  119 LRLNINARERRRMHDLNDALDELRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLN 187
[Close]
BLAST of e22/23 protein vs. Ensembl Cavefish
Match: olig3 (oligodendrocyte transcription factor 3 [Source:ZFIN;Acc:ZDB-GENE-080903-1])

HSP 1 Score: 102.449 bits (254), Expect = 1.047e-26
Identity = 49/86 (56.98%), Postives = 65/86 (75.58%), Query Frame = 2 
Query:   83 SKVNGQKYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTI 340
            +  N  KY   ++  +   + LRL IN RER+RMHDLN A+D LR V+PYAH PSVRKLSKIATLLLA+NYILM +S+L+E+++ +
Sbjct:   63 AGANAAKYRLKKQASEEEIQQLRLKINGRERKRMHDLNLAMDGLREVMPYAHGPSVRKLSKIATLLLARNYILMLSSSLDEMKRLV 148
[Close]
BLAST of e22/23 protein vs. Ensembl Cavefish
Match: olig2 (oligodendrocyte lineage transcription factor 2 [Source:ZFIN;Acc:ZDB-GENE-030131-4013])

HSP 1 Score: 100.523 bits (249), Expect = 2.847e-26
Identity = 56/106 (52.83%), Postives = 77/106 (72.64%), Query Frame = 2 
Query:   29 STAVSQSESDSNPNTM-MISKVNGQKYSSDRRYI-DSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTI 340
            S AVS ++ DS    + M SK        DR+ + ++  + +RL IN+RER+RMHDLN A+D LR V+PYAH PSVRKLSKIATLLLA+NYILM +++LEE+++ +
Sbjct:   33 SGAVSSTQGDSLALALKMFSK-------KDRKLLSENELQNIRLKINSRERKRMHDLNIAMDGLREVMPYAHGPSVRKLSKIATLLLARNYILMLSNSLEEMKRLV 131
[Close]
BLAST of e22/23 protein vs. Ensembl Cavefish
Match: olig4 (oligodendrocyte transcription factor 3-like [Source:NCBI gene;Acc:103045051])

HSP 1 Score: 99.3673 bits (246), Expect = 8.128e-26
Identity = 45/65 (69.23%), Postives = 57/65 (87.69%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTI 340
            LRL +N+RER+RMHDLN A+D LR V+PYA  PSVRKLSKI+TLLLA+NYILM +S+LEE++K +
Sbjct:   76 LRLKVNSRERKRMHDLNQAMDGLREVMPYAQGPSVRKLSKISTLLLARNYILMLSSSLEEMKKLV 140
[Close]
BLAST of e22/23 protein vs. Ensembl Sea Lamprey
Match: neurod1 (neuronal differentiation 1 [Source:NCBI gene;Acc:30169])

HSP 1 Score: 58.5362 bits (140), Expect = 3.776e-11
Identity = 34/56 (60.71%), Postives = 38/56 (67.86%), Query Frame = 2 
Query:  137 FKPLRLSINARERRRMHDLNDALDELRSVIP-YAHSPSVRKLSKIATLLLAKNYIL 301
            FK  R+  NARER RMH LN ALD LR V+P Y+     +KLSKI TL LAKNYI 
Sbjct:   21 FKMRRVKANARERNRMHGLNAALDNLRKVVPCYS---KTQKLSKIETLRLAKNYIW 73
[Close]
BLAST of e22/23 protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006843.1 (pep scaffold:Pmarinus_7.0:GL488467:178:1220:1 gene:ENSPMAG00000006182.1 transcript:ENSPMAT00000006843.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 54.299 bits (129), Expect = 5.354e-10
Identity = 29/58 (50.00%), Postives = 36/58 (62.07%), Query Frame = 2 
Query:  149 RLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALE 322
            R+  N RER RMH+LNDALD LR V+P    P   +L+KI TL  A NYI +    L+
Sbjct:   45 RMKANDRERNRMHNLNDALDALRGVLPTL--PDDARLTKIETLRFAYNYIWVLTETLQ 100
[Close]
BLAST of e22/23 protein vs. Ensembl Sea Lamprey
Match: scxa (scleraxis bHLH transcription factor a [Source:ZFIN;Acc:ZDB-GENE-060503-414])

HSP 1 Score: 50.447 bits (119), Expect = 9.085e-9
Identity = 27/52 (51.92%), Postives = 34/52 (65.38%), Query Frame = 2 
Query:  143 PLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYI 298
            P R + NARER R   +N A   LR++IP    P+ RKLSK+ TLLLA +YI
Sbjct:   36 PQRRAANARERDRTQSVNSAFTALRTLIPT--EPADRKLSKLETLLLASSYI 85
[Close]
BLAST of e22/23 protein vs. Ensembl Sea Lamprey
Match: TWIST1 (twist family bHLH transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:12428])

HSP 1 Score: 47.3654 bits (111), Expect = 1.651e-7
Identity = 31/78 (39.74%), Postives = 39/78 (50.00%), Query Frame = 2 
Query:  149 RLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQSRGYLS 382
            R   N RER+R   LN+A   LR +IP   S    KLSKI TL LA  YI      L E R+  +   S + ++  LS
Sbjct:   97 RFVANVRERQRTQSLNEAFASLRQIIPTLPS---DKLSKIQTLKLATRYIDFLYQVLAERRRWWVQCGSSYAAQERLS 171
[Close]
BLAST of e22/23 protein vs. Ensembl Sea Lamprey
Match: TWIST1 (twist family bHLH transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:12428])

HSP 1 Score: 47.3654 bits (111), Expect = 1.947e-7
Identity = 31/78 (39.74%), Postives = 39/78 (50.00%), Query Frame = 2 
Query:  149 RLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQSRGYLS 382
            R   N RER+R   LN+A   LR +IP   S    KLSKI TL LA  YI      L E R+  +   S + ++  LS
Sbjct:  115 RFVANVRERQRTQSLNEAFASLRQIIPTLPS---DKLSKIQTLKLATRYIDFLYQVLAERRRWWVQCGSSYAAQERLS 189
[Close]
BLAST of e22/23 protein vs. Ensembl Nematostella
Match: EDO42924 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZZ0])

HSP 1 Score: 49.2914 bits (116), Expect = 7.933e-9
Identity = 28/50 (56.00%), Postives = 33/50 (66.00%), Query Frame = 2 
Query:  149 RLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYI 298
            R + NARER R H +N A + LR +IP    PS RKLSKI TL LA +YI
Sbjct:    3 RQAANARERNRTHSVNAAFNALRLLIPT--EPSDRKLSKIETLRLASSYI 50
[Close]
BLAST of e22/23 protein vs. Ensembl Nematostella
Match: EDO36265 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJ29])

HSP 1 Score: 50.447 bits (119), Expect = 3.337e-8
Identity = 28/50 (56.00%), Postives = 30/50 (60.00%), Query Frame = 2 
Query:  149 RLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYI 298
            R    ARER RMH LNDA D LR  IP  +     K SKIATL LA +YI
Sbjct:   69 RECATARERSRMHSLNDAFDSLRKAIPKTNYNQEEKPSKIATLRLAIHYI 118
[Close]
BLAST of e22/23 protein vs. Ensembl Nematostella
Match: EDO30831 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZM2])

HSP 1 Score: 46.2098 bits (108), Expect = 4.268e-7
Identity = 31/66 (46.97%), Postives = 38/66 (57.58%), Query Frame = 2 
Query:  137 FKPLRLSINARERRRMHDLNDALDELRSVIP---YAHSPSVRKLSKIATLLLAKNYILMQASALEE 325
            F+ LR   NARER R   LN A D LR VIP       P  RKL++I TL LA +YI+  +  LE+
Sbjct:   56 FRQLRR--NARERERQGRLNSAFDVLRGVIPDYLSGKGPE-RKLTQIETLRLATHYIMALSEMLED 118
[Close]
BLAST of e22/23 protein vs. Ensembl Nematostella
Match: NVATO2 (Atonal-related protein 2; Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJ22])

HSP 1 Score: 45.8246 bits (107), Expect = 1.630e-6
Identity = 25/50 (50.00%), Postives = 31/50 (62.00%), Query Frame = 2 
Query:  149 RLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYI 298
            RL  N RERRRM  LN ALD L+  IP     S R+++K+  L +A NYI
Sbjct:  143 RLRANDRERRRMKSLNRALDSLKKCIPVPQ--SKRRVTKLEILRIACNYI 190
[Close]
BLAST of e22/23 protein vs. Ensembl Nematostella
Match: EDO36266 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJ30])

HSP 1 Score: 43.5134 bits (101), Expect = 1.731e-6
Identity = 28/61 (45.90%), Postives = 39/61 (63.93%), Query Frame = 2 
Query:  149 RLSINARERRRMHDLNDALDELRSVIP-YAHSPSVRKLSKIATLLLAKNYILMQASALEEL 328
            RL  NARER R+H +++A + LR  +P Y+     +KLSK+A L LA +YI    SAL +L
Sbjct:    4 RLIANARERSRIHTMSEAFESLRKAVPSYSQD---QKLSKLAILRLATSYI----SALADL 57
[Close]
BLAST of e22/23 protein vs. Ensembl Medaka
Match: bhlhe22 (basic helix-loop-helix family member e22 [Source:NCBI gene;Acc:101158239])

HSP 1 Score: 127.487 bits (319), Expect = 1.820e-36
Identity = 63/94 (67.02%), Postives = 75/94 (79.79%), Query Frame = 2 
Query:   71 TMMISKVNGQKYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMN 352
            T+   K +G K + +++        LRL+INARERRRMHDLNDALDELR+VIPYAHSPSVRKLSKIATLLLAKNYILMQA ALEE+R+ +  +N
Sbjct:  119 TVPAGKTDGGKKTKEQKM-------LRLNINARERRRMHDLNDALDELRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLN 205
[Close]
BLAST of e22/23 protein vs. Ensembl Medaka
Match: bhlhe23 (basic helix-loop-helix family member e23 [Source:NCBI gene;Acc:101164557])

HSP 1 Score: 124.405 bits (311), Expect = 1.041e-35
Identity = 59/69 (85.51%), Postives = 64/69 (92.75%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMN 352
            LRLSINARERRRMHDLNDALD LR+VIPYAHSPSVRKLSKIATLLLAKNYILMQA ALEE+R+ +  +N
Sbjct:  105 LRLSINARERRRMHDLNDALDGLRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLN 173
[Close]
BLAST of e22/23 protein vs. Ensembl Medaka
Match: olig4 (oligodendrocyte transcription factor 3 [Source:NCBI gene;Acc:101163780])

HSP 1 Score: 103.99 bits (258), Expect = 1.686e-27
Identity = 47/65 (72.31%), Postives = 58/65 (89.23%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTI 340
            LRL +N+RER+RMHDLN A+D LR V+PYAH PSVRKLSKI+TLLLA+NYILM +S+LEE+RK +
Sbjct:   73 LRLKVNSRERKRMHDLNQAMDGLREVMPYAHGPSVRKLSKISTLLLARNYILMLSSSLEEMRKLV 137
[Close]
BLAST of e22/23 protein vs. Ensembl Medaka
Match: olig2 (oligodendrocyte transcription factor 2 [Source:NCBI gene;Acc:101155730])

HSP 1 Score: 102.834 bits (255), Expect = 5.760e-27
Identity = 57/113 (50.44%), Postives = 79/113 (69.91%), Query Frame = 2 
Query:   29 STAVSQSESDSN---PNTMMISKVNGQ-----KYSSDRRYI-DSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTI 340
            S AVS ++SDS    P    +S  + +         DR+ + +S  + +RL IN+RER+RMHDLN A+D LR V+PYAH PSVRKLSKIATLLLA+NYILM +++LEE+++ +
Sbjct:   33 SGAVSSTQSDSPSEMPGLRGLSSADEELALRMAAKKDRKLLSESELQSIRLKINSRERKRMHDLNVAMDGLREVMPYAHGPSVRKLSKIATLLLARNYILMLSNSLEEMKRLV 145
[Close]
BLAST of e22/23 protein vs. Ensembl Medaka
Match: olig1 (oligodendrocyte transcription factor 1 [Source:ZFIN;Acc:ZDB-GENE-050107-2])

HSP 1 Score: 71.2478 bits (173), Expect = 7.403e-16
Identity = 36/73 (49.32%), Postives = 54/73 (73.97%), Query Frame = 2 
Query:  146 LRLSINARERRRMHDLNDALDELRSVIPY-AHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRF 361
            LR  IN+RER+RM DLN A+D LR V+P+   +P  R+LSKI+TL+LA+NYIL+  S+L+E+    +++ + F
Sbjct:   32 LRRKINSRERKRMQDLNIAMDALREVMPHQPGAPPGRRLSKISTLVLARNYILLLGSSLQEIWILDILLQNDF 104
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BLAST of e22/23 protein vs. Planmine SMEST
Match: SMESG000080379.1 (SMESG000080379.1)

HSP 1 Score: 316.235 bits (809), Expect = 4.593e-112
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 2 
Query:    2 MNEIEDCCGSTAVSQSESDSNPNTMMISKVNGQKYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQSRGYLSNIKYEQSTQKITSKELHSDNVSVQKHL 463
            MNEIEDCCGSTAVSQSESDSNPNTMMISKVNGQKYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQSRGYLSNIKYEQSTQKITSKELHSDNVSVQKHL
Sbjct:   24 MNEIEDCCGSTAVSQSESDSNPNTMMISKVNGQKYSSDRRYIDSIFKPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTIMMMNSRFQSRGYLSNIKYEQSTQKITSKELHSDNVSVQKHL 177
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BLAST of e22/23 protein vs. Planmine SMEST
Match: SMESG000060237.1 (SMESG000060237.1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.952e-26
Identity = 48/67 (71.64%), Postives = 57/67 (85.07%), Query Frame = 2 
Query:  140 KPLRLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALEELRKTI 340
            K +RL IN+RER+RMHDLN ALD LR VIPYA+ P+V+KLSKIATLLLAKNYILM  ++LEE +K I
Sbjct:   33 KEMRLKINSRERKRMHDLNSALDSLRQVIPYANGPTVKKLSKIATLLLAKNYILMLKNSLEESQKKI 99
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BLAST of e22/23 protein vs. Planmine SMEST
Match: SMESG000046205.1 (SMESG000046205.1)

HSP 1 Score: 53.1434 bits (126), Expect = 6.224e-10
Identity = 28/58 (48.28%), Postives = 38/58 (65.52%), Query Frame = 2 
Query:  149 RLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYILMQASALE 322
            R + N RER+RM+ LN A D+LR V+PY  S + +K+SK  TLL+A+ YI      LE
Sbjct:   22 RTAANDRERKRMYCLNRAFDQLRDVVPY--SSNQKKMSKFETLLMAQTYIETLVEMLE 77
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BLAST of e22/23 protein vs. Planmine SMEST
Match: SMESG000044960.1 (SMESG000044960.1)

HSP 1 Score: 53.5286 bits (127), Expect = 4.946e-9
Identity = 33/86 (38.37%), Postives = 49/86 (56.98%), Query Frame = 2 
Query:  149 RLSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYI-----LMQASALEELRKTIMMMNSRFQSRGYLSNIK 391
            R + N RER+RM  +NDA ++LR  IP    P  ++LSK+ TL +A  YI     +MQ    EE+ +   + N +FQ+    SN+K
Sbjct:   48 RQAANMRERKRMQSINDAFEKLRVHIPTM--PYEKRLSKVDTLRMAIGYINFLGGIMQVDQGEEIYEKTFIANDQFQTE---SNLK 128
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BLAST of e22/23 protein vs. Planmine SMEST
Match: SMESG000066244.1 (SMESG000066244.1)

HSP 1 Score: 48.521 bits (114), Expect = 3.289e-7
Identity = 28/51 (54.90%), Postives = 34/51 (66.67%), Query Frame = 2 
Query:  149 RLSINARERRRMHDLNDALDELRSVIP-YAHSPSVRKLSKIATLLLAKNYI 298
            R   N+RER RMH LN AL+ LR  +P + H+   +KLSKI TL LA NYI
Sbjct:   50 RARANSRERNRMHGLNHALESLRLHVPVFNHT---QKLSKIETLRLASNYI 97
The following BLAST results are available for this feature:
BLAST of e22/23 protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
BHLHE233.487e-3564.29basic helix-loop-helix family member e23 [Source:H... [more]
BHLHE234.584e-3564.29basic helix-loop-helix family member e23 [Source:H... [more]
BHLHE227.945e-3584.51basic helix-loop-helix family member e22 [Source:H... [more]
OLIG34.206e-2744.54oligodendrocyte transcription factor 3 [Source:HGN... [more]
OLIG29.738e-2670.77oligodendrocyte transcription factor 2 [Source:HGN... [more]
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BLAST of e22/23 protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hlh-321.950e-3057.28Helix Loop Helix [Source:UniProtKB/TrEMBL;Acc:Q7Y... [more]
hlh-177.633e-2975.71Helix Loop Helix [Source:UniProtKB/TrEMBL;Acc:O45... [more]
hlh-319.451e-2377.36Helix Loop Helix [Source:UniProtKB/TrEMBL;Acc:Q86... [more]
hlh-168.875e-1647.30Helix Loop Helix [Source:UniProtKB/TrEMBL;Acc:O45... [more]
cnd-14.946e-958.00Neurogenic differentiation factor 1 [Source:UniPr... [more]
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BLAST of e22/23 protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Oli1.558e-3584.29gene:FBgn0032651 transcript:FBtr0080986[more]
Oli1.558e-3584.29gene:FBgn0032651 transcript:FBtr0344781[more]
Oli1.558e-3584.29gene:FBgn0032651 transcript:FBtr0303253[more]
Oli1.558e-3584.29gene:FBgn0032651 transcript:FBtr0303252[more]
dimm1.172e-1062.75gene:FBgn0023091 transcript:FBtr0333354[more]
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BLAST of e22/23 protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
bhlhe223.982e-3685.51basic helix-loop-helix family, member e22 [Source:... [more]
bhlhe235.272e-3686.96basic helix-loop-helix family, member e23 [Source:... [more]
bhlhe221.030e-3585.51basic helix-loop-helix family, member e22 [Source:... [more]
olig31.893e-2844.74oligodendrocyte transcription factor 3 [Source:ZFI... [more]
si:ch211-196c10.131.425e-2752.78si:ch211-196c10.13 [Source:ZFIN;Acc:ZDB-GENE-13060... [more]
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BLAST of e22/23 protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
lbr4.531e-3571.59lamin B receptor [Source:Xenbase;Acc:XB-GENE-97930... [more]
zbed41.819e-2745.00zinc finger, BED-type containing 4 [Source:Xenbase... [more]
OLIG27.077e-2750.00oligodendrocyte transcription factor 2 [Source:HGN... [more]
gps12.248e-2463.08G protein pathway suppressor 1 [Source:Xenbase;Acc... [more]
znf4235.369e-1162.00zinc finger protein 423 [Source:Xenbase;Acc:XB-GEN... [more]
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BLAST of e22/23 protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Bhlhe235.022e-3685.51basic helix-loop-helix family, member e23 [Source:... [more]
Bhlhe222.109e-3584.51basic helix-loop-helix family, member e22 [Source:... [more]
Olig33.072e-2744.54oligodendrocyte transcription factor 3 [Source:MGI... [more]
Olig21.069e-2570.77oligodendrocyte transcription factor 2 [Source:MGI... [more]
Olig11.949e-1661.97oligodendrocyte transcription factor 1 [Source:MGI... [more]
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BLAST of e22/23 protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q8BGW3|BHE23_MOUSE3.513e-3585.51Class E basic helix-loop-helix protein 23 OS=Mus m... [more]
sp|Q71T09|BHE22_CHICK4.553e-3571.26Class E basic helix-loop-helix protein 22 OS=Gallu... [more]
sp|O09029|BHE22_MESAU1.574e-3484.51Class E basic helix-loop-helix protein 22 OS=Mesoc... [more]
sp|Q8NDY6|BHE23_HUMAN1.675e-3464.29Class E basic helix-loop-helix protein 23 OS=Homo ... [more]
sp|Q8C6A8|BHE22_MOUSE2.004e-3484.51Class E basic helix-loop-helix protein 22 OS=Mus m... [more]
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BLAST of e22/23 protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
U5YQ547.486e-10398.66E22/23 protein (Fragment) OS=Schmidtea mediterrane... [more]
A0A0A7M2A71.060e-9897.93BETA3 transcription factor (Fragment) OS=Schmidtea... [more]
A0A212FNL83.043e-3564.95BHLH domain-containing protein OS=Danaus plexippus... [more]
E9HGJ96.991e-3585.92BHLH domain-containing protein (Fragment) OS=Daphn... [more]
A0A4Z2D0L49.733e-3569.90Class E basic helix-loop-helix protein OS=Schistos... [more]
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BLAST of e22/23 protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
bhlhe239.777e-3685.51basic helix-loop-helix family, member e23 [Source:... [more]
bhlhe221.184e-3585.51basic helix-loop-helix family, member e22 [Source:... [more]
olig31.047e-2656.98oligodendrocyte transcription factor 3 [Source:ZFI... [more]
olig22.847e-2652.83oligodendrocyte lineage transcription factor 2 [So... [more]
olig48.128e-2669.23oligodendrocyte transcription factor 3-like [Sourc... [more]
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BLAST of e22/23 protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
neurod13.776e-1160.71neuronal differentiation 1 [Source:NCBI gene;Acc:3... [more]
ENSPMAT00000006843.15.354e-1050.00pep scaffold:Pmarinus_7.0:GL488467:178:1220:1 gene... [more]
scxa9.085e-951.92scleraxis bHLH transcription factor a [Source:ZFIN... [more]
TWIST11.651e-739.74twist family bHLH transcription factor 1 [Source:H... [more]
TWIST11.947e-739.74twist family bHLH transcription factor 1 [Source:H... [more]
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BLAST of e22/23 protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of e22/23 protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO429247.933e-956.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO362653.337e-856.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO308314.268e-746.97Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
NVATO21.630e-650.00Atonal-related protein 2; Predicted protein [Sour... [more]
EDO362661.731e-645.90Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of e22/23 protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
bhlhe221.820e-3667.02basic helix-loop-helix family member e22 [Source:N... [more]
bhlhe231.041e-3585.51basic helix-loop-helix family member e23 [Source:N... [more]
olig41.686e-2772.31oligodendrocyte transcription factor 3 [Source:NCB... [more]
olig25.760e-2750.44oligodendrocyte transcription factor 2 [Source:NCB... [more]
olig17.403e-1649.32oligodendrocyte transcription factor 1 [Source:ZFI... [more]
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BLAST of e22/23 protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000080379.14.593e-112100.00SMESG000080379.1[more]
SMESG000060237.11.952e-2671.64SMESG000060237.1[more]
SMESG000046205.16.224e-1048.28SMESG000046205.1[more]
SMESG000044960.14.946e-938.37SMESG000044960.1[more]
SMESG000066244.13.289e-754.90SMESG000066244.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30012010
GenBankAGZ94907.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012010 ID=SMED30012010|Name=e22/23 protein|organism=Schmidtea mediterranea sexual|type=transcript|length=503bp
CATGAATGAAATAGAAGACTGTTGCGGCTCGACTGCTGTCAGCCAGTCGG
AATCCGATTCAAATCCCAACACAATGATGATAAGTAAAGTCAATGGCCAA
AAATATTCCTCCGACAGACGATACATTGACAGTATTTTCAAGCCGCTGAG
ATTGAGTATCAATGCAAGAGAACGGCGTCGAATGCACGATTTGAACGATG
CGTTGGATGAATTGAGAAGCGTCATACCGTATGCTCACAGTCCATCGGTT
AGGAAGCTGTCGAAAATCGCTACTTTACTATTAGCGAAAAATTACATTTT
AATGCAAGCAAGTGCGCTGGAGGAATTGAGAAAGACAATAATGATGATGA
ACTCCCGGTTCCAGTCCCGAGGATATTTGAGTAATATAAAATATGAACAA
TCTACTCAGAAAATTACAAGTAAAGAATTACATTCCGATAACGTTTCAGT
TCAAAAGCATTTGTAATGACGAAATTTATAATAATAAAAATATTAGCAAT
TTG
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protein sequence of SMED30012010-orf-1

>SMED30012010-orf-1 ID=SMED30012010-orf-1|Name=SMED30012010-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=155bp
MNEIEDCCGSTAVSQSESDSNPNTMMISKVNGQKYSSDRRYIDSIFKPLR
LSINARERRRMHDLNDALDELRSVIPYAHSPSVRKLSKIATLLLAKNYIL
MQASALEELRKTIMMMNSRFQSRGYLSNIKYEQSTQKITSKELHSDNVSV
QKHL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000103central nervous system
PLANA:0002142parenchyma
Vocabulary: INTERPRO
TermDefinition
IPR036638HLH_DNA-bd_sf
IPR011598bHLH_dom
Vocabulary: molecular function
TermDefinition
GO:0046983protein dimerization activity
GO:0000981RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0003682chromatin binding
GO:0005515protein binding
GO:0042803protein homodimerization activity
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
Vocabulary: biological process
TermDefinition
GO:0006351transcription, DNA-templated
GO:0006355regulation of transcription, DNA-templated
GO:0006357regulation of transcription from RNA polymerase II promoter
GO:0007399nervous system development
GO:0021540corpus callosum morphogenesis
GO:0021796cerebral cortex regionalization
GO:0021952central nervous system projection neuron axonogenesis
GO:0021957corticospinal tract morphogenesis
GO:0021960anterior commissure morphogenesis
GO:0022008neurogenesis
GO:0030182neuron differentiation
GO:0045892negative regulation of transcription, DNA-templated
GO:0060040retinal bipolar neuron differentiation
GO:0060042retina morphogenesis in camera-type eye
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 54..74
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 7..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availablePANTHERPTHR19290:SF104HELIX LOOP HELIXcoord: 13..134
NoneNo IPR availablePANTHERPTHR19290BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATEDcoord: 13..134
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 54..108
e-value: 3.3E-16
score: 69.9
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 50..101
e-value: 4.1E-15
score: 55.5
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 48..102
score: 15.612
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainCDDcd00083HLHcoord: 50..107
e-value: 7.27897E-13
score: 57.9943
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3DG3DSA:4.10.280.10coord: 46..107
e-value: 1.4E-18
score: 68.2
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILYSSF47459HLH, helix-loop-helix DNA-binding domaincoord: 48..120