mannosyl phosphorylinositol ceramide synthase CSH1-like

Overview
Namemannosyl phosphorylinositol ceramide synthase CSH1-like
Smed IDSMED30012001
Length (bp)568
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of mannosyl phosphorylinositol ceramide synthase CSH1-like (SMED30012001) t-SNE clustered cells

Violin plots show distribution of expression levels for mannosyl phosphorylinositol ceramide synthase CSH1-like (SMED30012001) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of mannosyl phosphorylinositol ceramide synthase CSH1-like (SMED30012001) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for mannosyl phosphorylinositol ceramide synthase CSH1-like (SMED30012001) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30012001

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30012001h1SMcG0001692 dd_Smed_v4_13253_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30012001h1SMcG0001692 dd_Smed_v4_13253_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30012001h1SMcG0001692 OX_Smed_1.0.02716ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. UniProt/SwissProt
Match: sp|P33300|SUR1_YEAST (Mannosyl phosphorylinositol ceramide synthase SUR1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUR1 PE=1 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 9.332e-10
Identity = 45/133 (33.83%), Postives = 62/133 (46.62%), Query Frame = 3 
Query:    6 INRADGSRYFYLVHYGGIYTDLDNECLRPFEHLLKNYSLVFGAMKGQYRGTKLKEGYVQNSFMYSKPRQTFWLELISDVMNFNGKQYPE-----ASTGPILLSNRIDLYRK-RQPDNNGIIIYLPHYFNPFSW 386
            I RAD  RYF L HYGG+Y DLD+ C R  + LL        A     R T      V N  M S PR  F+L+ +  + +++   +        STGP+ LS     Y++ R P N  + I  P Y+   S+
Sbjct:  118 IERADAIRYFILSHYGGVYIDLDDGCERKLDPLL--------AFPAFLRKT--SPLGVSNDVMGSVPRHPFFLKALKSLKHYDKYWFIPYMTIMGSTGPLFLSVIWKQYKRWRIPKNGTVRILQPAYYKMHSY 240
[Close]
BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. UniProt/SwissProt
Match: sp|P38287|CSH1_YEAST (Mannosyl phosphorylinositol ceramide synthase CSH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CSH1 PE=1 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.120e-9
Identity = 42/126 (33.33%), Postives = 58/126 (46.03%), Query Frame = 3 
Query:    6 INRADGSRYFYLVHYGGIYTDLDNECLRPFEHLLKNYSLVFGAMKGQYRGTKLKEGYVQNSFMYSKPRQTFWLELISDVMNFNGKQYPE-----ASTGPILLSNRIDLYRK--RQPDNNGIIIYLP 362
            I RAD  RYF L HYGGIY DLD+ C R  + LLK  + +           K     V N  M S PR  F+L++I  + ++    Y        STGP+ +S     Y++     +N  + I  P
Sbjct:  125 IERADAIRYFILSHYGGIYIDLDDGCERRLDPLLKVPAFL----------RKTSPTGVSNDVMGSVPRHPFFLKVIKSLKHYKKNWYIPYMTIMGSTGPLFISVVWKQYKRWSNTAENGAVRILQP 240
[Close]
BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. UniProt/SwissProt
Match: sp|O14084|IMT1_SCHPO (Inositol phosphoceramide mannosyltransferase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=imt1 PE=3 SV=2)

HSP 1 Score: 56.225 bits (134), Expect = 2.166e-8
Identity = 34/104 (32.69%), Postives = 52/104 (50.00%), Query Frame = 3 
Query:    3 NINRADGSRYFYLVHYGGIYTDLDNECLRPFEHLLKNYSLVFGAMKGQYRGTKLKEGYVQNSFMYSKPRQTFWLELISDVMNFNGK---QYPEA--STGPILLS 299
            NI + D +RYF L HYGG+Y DLD  C +P + LL   + +  + +          GY  N +  + P+  F  + I ++  FN +   +YP    S GP+ LS
Sbjct:  129 NIQKFDAARYFILYHYGGVYMDLDIGCKKPMDPLLSKATFILPSTE--------PIGY-SNDWFAATPKHPFLYQAIHNLSKFNHRYFTKYPTVFLSAGPLFLS 223
[Close]
BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. TrEMBL
Match: A0A1I8FXP5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 2.179e-38
Identity = 78/182 (42.86%), Postives = 108/182 (59.34%), Query Frame = 3 
Query:    3 NINRADGSRYFYLVHYGGIYTDLDNECLRPFEHLLKNYSLVFGAMKGQ-----YRGTKLKEGYVQNSFMYSKPRQTFWLELISDVMNFNGKQYPEASTGPILLSNRIDLYRKRQPDNNG------IIIYLPHYFNPFSWIKPENVDPNCKSHNKMTEENLSKCRQSMIKKGSYIVQYHAHTW 515
             + +AD SR FY+ HYGG+Y DLD ECL  FEH+L N SL FGAM G+        + + EG ++NSFMYS+P+  F+ ELI  V+N   +  P  ++GP L+   I   RKR  D         + IY PH FNP+SWI+  +  P+C+     +EE+L++CR S   K SY++QYH   W
Sbjct:  150 GVQKADVSRLFYMYHYGGLYCDLDCECLANFEHMLANTSLAFGAMDGKMIYSSSDSSYVDEGQIENSFMYSRPKHPFFWELIR-VLNTTTEGLPIMTSGPYLIMRGIHSARKRGLDKTKFDGLPYLTIYPPHLFNPYSWIR--HNAPSCRLFGDFSEESLARCRNSF--KTSYVIQYHTQVW 326
[Close]
BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. TrEMBL
Match: A0A1I8GI25 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008668g1 PE=4 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 2.348e-38
Identity = 78/182 (42.86%), Postives = 108/182 (59.34%), Query Frame = 3 
Query:    3 NINRADGSRYFYLVHYGGIYTDLDNECLRPFEHLLKNYSLVFGAMKGQ-----YRGTKLKEGYVQNSFMYSKPRQTFWLELISDVMNFNGKQYPEASTGPILLSNRIDLYRKRQPDNNG------IIIYLPHYFNPFSWIKPENVDPNCKSHNKMTEENLSKCRQSMIKKGSYIVQYHAHTW 515
             + +AD SR FY+ HYGG+Y DLD ECL  FEH+L N SL FGAM G+        + + EG ++NSFMYS+P+  F+ ELI  V+N   +  P  ++GP L+   I   RKR  D         + IY PH FNP+SWI+  +  P+C+     +EE+L++CR S   K SY++QYH   W
Sbjct:  150 GVQKADVSRLFYMYHYGGLYCDLDCECLANFEHMLANTSLAFGAMDGKMIYSSSDSSYVDEGQIENSFMYSRPKHPFFWELIR-VLNTTTEGLPIMTSGPYLIMRGIHSARKRGLDKTKFDGLPYLTIYPPHLFNPYSWIR--HNAPSCRLFGDFSEESLARCRNSF--KTSYVIQYHTQVW 326
[Close]
BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. TrEMBL
Match: A0A267GLX5 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008668g2 PE=4 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 4.123e-38
Identity = 78/182 (42.86%), Postives = 108/182 (59.34%), Query Frame = 3 
Query:    3 NINRADGSRYFYLVHYGGIYTDLDNECLRPFEHLLKNYSLVFGAMKGQ-----YRGTKLKEGYVQNSFMYSKPRQTFWLELISDVMNFNGKQYPEASTGPILLSNRIDLYRKRQPDNNG------IIIYLPHYFNPFSWIKPENVDPNCKSHNKMTEENLSKCRQSMIKKGSYIVQYHAHTW 515
             + +AD SR FY+ HYGG+Y DLD ECL  FEH+L N SL FGAM G+        + + EG ++NSFMYS+P+  F+ ELI  V+N   +  P  ++GP L+   I   RKR  D         + IY PH FNP+SWI+  +  P+C+     +EE+L++CR S   K SY++QYH   W
Sbjct:  222 GVQKADVSRLFYMYHYGGLYCDLDCECLANFEHMLANTSLAFGAMDGKMIYSSSDSSYVDEGQIENSFMYSRPKHPFFWELIR-VLNTTTEGLPIMTSGPYLIMRGIHSARKRGLDKTKFDGLPYLTIYPPHLFNPYSWIR--HNAPSCRLFGDFSEESLARCRNSF--KTSYVIQYHTQVW 398
[Close]
BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. TrEMBL
Match: A0A267FKP7 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008668g3 PE=4 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 4.383e-38
Identity = 78/182 (42.86%), Postives = 108/182 (59.34%), Query Frame = 3 
Query:    3 NINRADGSRYFYLVHYGGIYTDLDNECLRPFEHLLKNYSLVFGAMKGQ-----YRGTKLKEGYVQNSFMYSKPRQTFWLELISDVMNFNGKQYPEASTGPILLSNRIDLYRKRQPDNNG------IIIYLPHYFNPFSWIKPENVDPNCKSHNKMTEENLSKCRQSMIKKGSYIVQYHAHTW 515
             + +AD SR FY+ HYGG+Y DLD ECL  FEH+L N SL FGAM G+        + + EG ++NSFMYS+P+  F+ ELI  V+N   +  P  ++GP L+   I   RKR  D         + IY PH FNP+SWI+  +  P+C+     +EE+L++CR S   K SY++QYH   W
Sbjct:  219 GVQKADVSRLFYMYHYGGLYCDLDCECLANFEHMLANTSLAFGAMDGKMIYSSSDSSYVDEGQIENSFMYSRPKHPFFWELIR-VLNTTTEGLPIMTSGPYLIMRGIHSARKRGLDKTTFDGLPYLTIYPPHLFNPYSWIR--HNAPSCRLFGDFSEESLARCRNSF--KTSYVIQYHTQVW 395
[Close]
BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. TrEMBL
Match: A0A1S3KDX7 (mannosyl phosphorylinositol ceramide synthase CSH1-like OS=Lingula unguis OX=7574 GN=LOC106181098 PE=4 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 2.484e-36
Identity = 74/174 (42.53%), Postives = 101/174 (58.05%), Query Frame = 3 
Query:    3 NINRADGSRYFYLVHYGGIYTDLDNECLRPFEHLLKNYSLVFGAMK--GQYRGTKLKEGYVQNSFMYSKPRQTFWLELISDVMNFNGKQYPEASTGPILLSNRIDLYRKRQPDNNGIIIYLPHYFNPFSWIKPENVDPNCKSHNKMTEENLSKCRQSMIKKGSYIVQYHAHTWG 518
             I RAD +RYF L H+GG+Y DLD ECLRPF+HLL +  LVF +M+   ++        YVQNSFM S     FW+E+I ++   +G Q+P+ +TGP L+   +D  R     ++   IY P YFNPFSW   +N  P CK  N MT+  +  C+      G+Y + YH  TWG
Sbjct:  192 EIKRADAARYFILYHFGGLYVDLDYECLRPFDHLL-DIPLVFDSMRVGNKWAYANGTFPYVQNSFMASTKGHPFWMEVIQEMTTRSGDQWPQGATGPYLI---MDTLRNYSQSHSDFRIYPPQYFNPFSWDIYDN--PYCKDLNAMTDREIDLCKSQY--PGAYAISYHTQTWG 357
[Close]
BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. Ensembl Yeast
Match: SUR1 (Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication [Source:SGD;Acc:S000005978])

HSP 1 Score: 60.077 bits (144), Expect = 1.344e-11
Identity = 45/133 (33.83%), Postives = 62/133 (46.62%), Query Frame = 3 
Query:    6 INRADGSRYFYLVHYGGIYTDLDNECLRPFEHLLKNYSLVFGAMKGQYRGTKLKEGYVQNSFMYSKPRQTFWLELISDVMNFNGKQYPE-----ASTGPILLSNRIDLYRK-RQPDNNGIIIYLPHYFNPFSW 386
            I RAD  RYF L HYGG+Y DLD+ C R  + LL        A     R T      V N  M S PR  F+L+ +  + +++   +        STGP+ LS     Y++ R P N  + I  P Y+   S+
Sbjct:  118 IERADAIRYFILSHYGGVYIDLDDGCERKLDPLL--------AFPAFLRKT--SPLGVSNDVMGSVPRHPFFLKALKSLKHYDKYWFIPYMTIMGSTGPLFLSVIWKQYKRWRIPKNGTVRILQPAYYKMHSY 240
[Close]
BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. Ensembl Yeast
Match: CSH1 (Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication [Source:SGD;Acc:S000000365])

HSP 1 Score: 59.3066 bits (142), Expect = 3.054e-11
Identity = 42/126 (33.33%), Postives = 58/126 (46.03%), Query Frame = 3 
Query:    6 INRADGSRYFYLVHYGGIYTDLDNECLRPFEHLLKNYSLVFGAMKGQYRGTKLKEGYVQNSFMYSKPRQTFWLELISDVMNFNGKQYPE-----ASTGPILLSNRIDLYRK--RQPDNNGIIIYLP 362
            I RAD  RYF L HYGGIY DLD+ C R  + LLK  + +           K     V N  M S PR  F+L++I  + ++    Y        STGP+ +S     Y++     +N  + I  P
Sbjct:  125 IERADAIRYFILSHYGGIYIDLDDGCERRLDPLLKVPAFL----------RKTSPTGVSNDVMGSVPRHPFFLKVIKSLKHYKKNWYIPYMTIMGSTGPLFISVVWKQYKRWSNTAENGAVRILQP 240
[Close]
BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. Planmine SMEST
Match: SMESG000059812.1 (SMESG000059812.1)

HSP 1 Score: 273.478 bits (698), Expect = 2.098e-94
Identity = 125/172 (72.67%), Postives = 145/172 (84.30%), Query Frame = 3 
Query:    3 NINRADGSRYFYLVHYGGIYTDLDNECLRPFEHLLKNYSLVFGAMKGQYRGTKLKEGYVQNSFMYSKPRQTFWLELISDVMNFNGKQYPEASTGPILLSNRIDLYRKRQPDNNGIIIYLPHYFNPFSWIKPENVDPNCKSHNKMTEENLSKCRQSMIKKGSYIVQYHAHTWG 518
            NI RAD SRYFYL HYGGIYTDLDNECLRPFEHLL+NYS+VFGAMKGQY GT LKEGYVQNSFMYS+PRQTFW+EL++D++ F G    E  TGP+LLS RI L+R+R   ++ ++IY P+YFNPFSWI    VDPNCKSHN+MT ENL+KCR SMI KGSY+VQYHAH+WG
Sbjct:   29 NIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFKGTNSVEVQTGPVLLSKRIVLFRERLNSSDYMMIYSPNYFNPFSWIDKSKVDPNCKSHNQMTRENLNKCRSSMISKGSYVVQYHAHSWG 200
[Close]
BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. Planmine SMEST
Match: SMESG000045292.1 (SMESG000045292.1)

HSP 1 Score: 273.863 bits (699), Expect = 2.035e-93
Identity = 127/172 (73.84%), Postives = 144/172 (83.72%), Query Frame = 3 
Query:    3 NINRADGSRYFYLVHYGGIYTDLDNECLRPFEHLLKNYSLVFGAMKGQYRGTKLKEGYVQNSFMYSKPRQTFWLELISDVMNFNGKQYPEASTGPILLSNRIDLYRKRQPDNNGIIIYLPHYFNPFSWIKPENVDPNCKSHNKMTEENLSKCRQSMIKKGSYIVQYHAHTWG 518
            NI RAD SRYFYL HYGGIYTDLDNECLRPFEHLL+NYSLVFGAMKGQY GT LKEGYVQNSFMYS+PRQTFW+EL++D++   G    E  TGP+LLS RI LYR+R   ++ ++IY P+YFNPFSWI    VDPNCKSHNKMT ENL+KCR SMI KGSY+VQYHAH+WG
Sbjct:  113 NIFRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSLVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMSKGTGSVEVQTGPVLLSKRIALYRERLNSSDDMMIYSPNYFNPFSWIDNSKVDPNCKSHNKMTLENLNKCRSSMISKGSYVVQYHAHSWG 284
[Close]
BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. Planmine SMEST
Match: SMESG000045267.1 (SMESG000045267.1)

HSP 1 Score: 273.092 bits (697), Expect = 4.094e-93
Identity = 125/172 (72.67%), Postives = 144/172 (83.72%), Query Frame = 3 
Query:    3 NINRADGSRYFYLVHYGGIYTDLDNECLRPFEHLLKNYSLVFGAMKGQYRGTKLKEGYVQNSFMYSKPRQTFWLELISDVMNFNGKQYPEASTGPILLSNRIDLYRKRQPDNNGIIIYLPHYFNPFSWIKPENVDPNCKSHNKMTEENLSKCRQSMIKKGSYIVQYHAHTWG 518
            NI RAD SRYFYL HYGGIYTDLDNECLRPFEHLL+NYS+VFGAMKGQY GT LKEGYVQNSFMYS+PRQTFW+EL++D++ F G    E  TGP+LLS RI LYR+R   ++ ++IY P+YFNPFSWI    VD NCKSHN+MT ENL+KCR SMI KGSY+VQYHAH+WG
Sbjct:  113 NIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFKGTDSVEVQTGPVLLSKRIALYRERLNSSDDMMIYSPNYFNPFSWIDNSKVDLNCKSHNQMTRENLNKCRSSMISKGSYVVQYHAHSWG 284
[Close]
BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. Planmine SMEST
Match: SMESG000045480.1 (SMESG000045480.1)

HSP 1 Score: 271.552 bits (693), Expect = 1.536e-92
Identity = 124/172 (72.09%), Postives = 144/172 (83.72%), Query Frame = 3 
Query:    3 NINRADGSRYFYLVHYGGIYTDLDNECLRPFEHLLKNYSLVFGAMKGQYRGTKLKEGYVQNSFMYSKPRQTFWLELISDVMNFNGKQYPEASTGPILLSNRIDLYRKRQPDNNGIIIYLPHYFNPFSWIKPENVDPNCKSHNKMTEENLSKCRQSMIKKGSYIVQYHAHTWG 518
            NI RAD SRYFYL HYGGIYTDLDNECLRPFEHLL+NYS+VFGAMKGQY GT LKEGYVQNSFMYS+PRQTFW+EL++D++ F G    E  TGP+LLS RI LYR+R   ++ ++IY P+YFNPFSWI    VDPNCKS N+MT ENL+KCR SM+ KGSY+VQYHAH+WG
Sbjct:  113 NIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFKGTDSVEVQTGPVLLSKRIALYRERLNSSDDMMIYSPNYFNPFSWIDNSKVDPNCKSPNQMTRENLNKCRSSMLSKGSYVVQYHAHSWG 284
[Close]
BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. Planmine SMEST
Match: SMESG000037990.1 (SMESG000037990.1)

HSP 1 Score: 260.766 bits (665), Expect = 2.853e-88
Identity = 121/174 (69.54%), Postives = 140/174 (80.46%), Query Frame = 3 
Query:    3 NINRADGSRYFYLVHYGGIYTDLDNECLRPFEHLLKNYSLVFGAMKGQYRGTKLKEGYVQNSFMYSKPRQTFWLELISDVMNFNGKQYPEASTGPILLSNR--IDLYRKRQPDNNGIIIYLPHYFNPFSWIKPENVDPNCKSHNKMTEENLSKCRQSMIKKGSYIVQYHAHTWG 518
            NI R D SRYFYL HYGGIYTDLDNECLRPFEHLL+NYS+VFGAMKGQY GT LKEGYVQNSFMYS+PRQTFW+EL++D++ FNG    E  TGP+LLS    I LYR+R   ++ ++IY P+YFNP SWI    VDPNCK HN+MT ENL KC+ SMI  GSY+VQYHAH+WG
Sbjct:  113 NIYRVDVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFNGTDSVEVQTGPVLLSKSFIIALYRERLNSSDDMMIYSPNYFNPLSWIIKSKVDPNCKFHNQMTLENLKKCKSSMISTGSYVVQYHAHSWG 286
The following BLAST results are available for this feature:
BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|P33300|SUR1_YEAST9.332e-1033.83Mannosyl phosphorylinositol ceramide synthase SUR1... [more]
sp|P38287|CSH1_YEAST2.120e-933.33Mannosyl phosphorylinositol ceramide synthase CSH1... [more]
sp|O14084|IMT1_SCHPO2.166e-832.69Inositol phosphoceramide mannosyltransferase 1 OS=... [more]
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BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8FXP52.179e-3842.86Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8GI252.348e-3842.86Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267GLX54.123e-3842.86Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A267FKP74.383e-3842.86Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A1S3KDX72.484e-3642.53mannosyl phosphorylinositol ceramide synthase CSH1... [more]
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BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
SUR11.344e-1133.83Mannosylinositol phosphorylceramide (MIPC) synthas... [more]
CSH13.054e-1133.33Mannosylinositol phosphorylceramide (MIPC) synthas... [more]
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BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of mannosyl phosphorylinositol ceramide synthase CSH1-like vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000059812.12.098e-9472.67SMESG000059812.1[more]
SMESG000045292.12.035e-9373.84SMESG000045292.1[more]
SMESG000045267.14.094e-9372.67SMESG000045267.1[more]
SMESG000045480.11.536e-9272.09SMESG000045480.1[more]
SMESG000037990.12.853e-8869.54SMESG000037990.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30012001 ID=SMED30012001|Name=mannosyl phosphorylinositol ceramide synthase CSH1-like|organism=Schmidtea mediterranea sexual|type=transcript|length=568bp
AAAACATTAACAGAGCCGATGGCAGTCGATATTTCTATTTGGTTCATTAT
GGAGGGATTTATACTGATCTCGACAATGAATGTTTGCGACCGTTTGAACA
CTTGTTGAAAAATTATTCTCTCGTGTTCGGAGCAATGAAAGGACAGTATC
GGGGCACAAAATTAAAAGAAGGATACGTTCAGAACTCGTTCATGTATTCG
AAACCTCGACAAACATTTTGGTTGGAATTGATCTCAGATGTCATGAATTT
TAATGGTAAACAGTATCCAGAAGCATCAACCGGTCCGATTCTTTTGTCAA
ACAGGATCGACTTGTACCGTAAACGGCAGCCGGACAACAATGGAATAATT
ATTTATTTGCCCCATTATTTCAATCCATTCAGTTGGATTAAGCCAGAAAA
TGTTGATCCAAACTGTAAATCTCACAATAAAATGACGGAGGAAAATTTAT
CAAAATGCCGACAATCTATGATTAAAAAAGGCTCATATATTGTTCAGTAT
CACGCTCACACTTGGGGATAAAAATTTTCCCAGATTATTTTTTATTGTAT
TCGAAATATTGTAAACCA
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protein sequence of SMED30012001-orf-1

>SMED30012001-orf-1 ID=SMED30012001-orf-1|Name=SMED30012001-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=173bp
NINRADGSRYFYLVHYGGIYTDLDNECLRPFEHLLKNYSLVFGAMKGQYR
GTKLKEGYVQNSFMYSKPRQTFWLELISDVMNFNGKQYPEASTGPILLSN
RIDLYRKRQPDNNGIIIYLPHYFNPFSWIKPENVDPNCKSHNKMTEENLS
KCRQSMIKKGSYIVQYHAHTWG*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR007577GlycoTrfase_DXD_sugar-bd_CS
IPR029044Nucleotide-diphossugar_trans
Vocabulary: molecular function
TermDefinition
GO:0000030mannosyltransferase activity
GO:0005537mannose binding
GO:0016740transferase activity
GO:0016757transferase activity, transferring glycosyl groups
Vocabulary: cellular component
TermDefinition
GO:0005794Golgi apparatus
GO:0016020membrane
GO:0016021integral component of membrane
GO:0032588trans-Golgi network membrane
GO:0033106cis-Golgi network membrane
Vocabulary: biological process
TermDefinition
GO:0051999mannosyl-inositol phosphorylceramide biosynthetic process
GO:0097502mannosylation