SMED30011999

Overview
NameSMED30011999
Smed IDSMED30011999
Length (bp)360
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30011999

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30011999SMESG000064314.1 dd_Smed_v4_11640_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30011999SMESG000064314.1 dd_Smed_v4_11640_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30011999SMESG000064314.1 Contig53784newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30011999SMESG000064314.1 Contig53784uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30011999SMESG000064303.1 SMESG000064314.1 Contig19558newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30011999SMESG000064303.1 SMESG000064314.1 Contig19558uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
reproductive organSMED30011999SMESG000064314.1 Contig53784newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30011999SMESG000064314.1 Contig53784uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30011999 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000055:312654..313013 -1764
v31.000055:313666..314997 +1382
v31.000055:313300..314875 -777
v31.000055:314761..315117 -766
v31.000055:313546..313894 -764
Homology
[Close]
BLAST of SMED30011999 vs. Planmine SMEST
Match: SMESG000064314.1 (SMESG000064314.1)

HSP 1 Score: 223.016 bits (567), Expect = 1.769e-68
Identity = 115/119 (96.64%), Postives = 115/119 (96.64%), Query Frame = 3 
Query:    3 DLNEETDHSADQIEREPVESNINEADEEEITQEKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            DLNEETDHSADQIEREP ESNINEA EEEITQEK NLEDLSKVYEKDDSGD NESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL
Sbjct: 3278 DLNEETDHSADQIEREPAESNINEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 3396

HSP 2 Score: 115.931 bits (289), Expect = 4.982e-31
Identity = 73/117 (62.39%), Postives = 84/117 (71.79%), Query Frame = 3 
Query:   33 DQIERE--PVESNINEADEEEITQEK------LNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            D+I+R   P ES + E    EI Q K      +NL ++SKV EK  S D NE+EPSE  INEVDEGEPKQQEQVNPE VSKVNEKDNSYDPNE  LA++ I EIDQG+NNI D VN+
Sbjct: 2943 DEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNV 3059

HSP 3 Score: 112.849 bits (281), Expect = 5.216e-30
Identity = 80/157 (50.96%), Postives = 90/157 (57.32%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEE-ITQEKLNLEDLSKVYEKDDSGDPNE-----------------------------------------SEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            E  HSAD  E EP E NINE DE E   QE++N E +SKV EKD+S DPNE                                         SEPSE  INEVDEGEPKQQEQ NPEEV KVN+KDNS DPN+S L E +I+EIDQG+NNI D VN+
Sbjct: 2985 EKGHSADLNENEPSEYNINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSEDNINEVDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNV 3141

HSP 4 Score: 102.834 bits (255), Expect = 1.643e-26
Identity = 61/102 (59.80%), Postives = 77/102 (75.49%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEEITQEKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEI 320
            ET++S DQIERE  ESNI+E DEEEITQEK NL+++SKV E D S DPNESEPS S INE+ +GE    ++VN ++VSK +E D+S DPN+S   + YI EI
Sbjct: 2621 ETEYSVDQIERESAESNIDEVDEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEI 2722

HSP 5 Score: 102.834 bits (255), Expect = 1.957e-26
Identity = 64/115 (55.65%), Postives = 78/115 (67.83%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEEITQEKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            E D+S D    EP +SNINE   + +T EK NLE++SK  E DDS DPNE E  +  I  +DEGE    E+ N  E+SK++E D S  PNES LAE+YI+EIDQ KNNITDPVNL
Sbjct: 2864 ENDYSRDPNGSEPDDSNINELGGK-MTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNL 2977

HSP 6 Score: 99.3673 bits (246), Expect = 2.562e-25
Identity = 65/115 (56.52%), Postives = 79/115 (68.70%), Query Frame = 3 
Query:   12 EETDHSADQIEREPVESNINEADEEE-ITQEKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPV 353
            EETDH+ +  E EP ESNI E DE E   QE++N E++SKV EK DSGD NESE  E  I  V EGE    E+ N  E+SK++E D S   NES LAE+YI+EIDQG+NN+ DPV
Sbjct: 3148 EETDHTGNLNESEPYESNIKEVDEGEPKQQEQVNPEEVSKVNEKGDSGDTNESEIVEPNIKNVYEGETDISEEPNVNELSKIDEIDRSVYTNESKLAEHYIIEIDQGENNVADPV 3262

HSP 7 Score: 98.2117 bits (243), Expect = 8.213e-25
Identity = 66/116 (56.90%), Postives = 82/116 (70.69%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEEITQEKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQ-VNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            E D+S D  E EP ESNINE   E +T EK NL + SK++EKDDS DPN+SE +E  IN VDE EPKQQ+Q  N EE SKV+EKDNS DPNE+ L E +I  I +G+ NI++  N+
Sbjct: 3445 EKDNSNDPSEIEPAESNINEFGGE-MTNEKFNLVEASKIFEKDDSFDPNKSELAELNINGVDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNISEESNV 3559

HSP 8 Score: 96.6709 bits (239), Expect = 2.633e-24
Identity = 64/116 (55.17%), Postives = 83/116 (71.55%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEEITQEK-LNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            ETDHSA+  + EP ES INE DE +  Q+K ++L+ +SKV +KD S + NESE +ES I+EVDE E KQ EQVN EEVS V+  D+S +PNE  LAE  I+E+D  K NI++ VNL
Sbjct: 2130 ETDHSANSNKSEPSESKINEVDEADWKQQKKVDLDGVSKVDKKDRSINLNESELAESNISEVDERETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIEVDVDKRNISEKVNL 2245

HSP 9 Score: 91.2781 bits (225), Expect = 1.922e-22
Identity = 59/115 (51.30%), Postives = 80/115 (69.57%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEEITQEKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            E+DHS D  + EP +S INE  E EIT +K+NL+++ KV E +DS DPNE E  ES I  V+EG+    E+ N  E S ++E D S DPN+S L+E+YI+EIDQ + NI+DPVN+
Sbjct: 2702 ESDHSVDPNKSEPGKSYINEI-EGEITHKKINLDEVPKVDENNDSVDPNEIEIVESNIENVEEGKTNLSEESNINESSTIDEPDLSVDPNKSELSEHYIIEIDQVEKNISDPVNV 2815

HSP 10 Score: 90.8929 bits (224), Expect = 2.522e-22
Identity = 70/155 (45.16%), Postives = 84/155 (54.19%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEEITQEKLNLEDLSKVYEKDDSGDP----------------------------------------NESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            ETD S D  E EP ESNIN+ DEEE TQE+L  E++SKV EKD S DP                                        NES+P ES I EVD+GE  QQEQVN EEVS V++  +S D NES LA   I++ID+G+ NI D VN+
Sbjct: 1855 ETDPSVDLNENEPTESNINKVDEEETTQEQLISEEVSKVGEKDVSVDPNESDFVEHNIKKVDEDEKKQHKQINLDVSKLDETDHSVDTNESKPVESNIIEVDDGEWTQQEQVNSEEVSNVDKTYHSVDQNESELANQNIIKIDKGEKNILDQVNV 2009

HSP 11 Score: 87.0409 bits (214), Expect = 6.910e-21
Identity = 65/152 (42.76%), Postives = 84/152 (55.26%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEEITQ-EKLNLEDLSKV------------------------------------YEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            ETDHS D  E +PVESNI E D+ E TQ E++N E++S V                                    Y+ D S DPN+ + +E  INEVD GEPKQQEQVN +EVSKV+ KD S D NES L E  ++EID+ + NI++  N+
Sbjct: 1935 ETDHSVDTNESKPVESNIIEVDDGEWTQQEQVNSEEVSNVDKTYHSVDQNESELANQNIIKIDKGEKNILDQVNVLYKTDHSVDPNKIKLAEFNINEVDNGEPKQQEQVNSDEVSKVDVKDKSVDTNESELCEPNVIEIDEVEKNISEKSNV 2086

HSP 12 Score: 81.6481 bits (200), Expect = 4.116e-19
Identity = 56/110 (50.91%), Postives = 75/110 (68.18%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEEI-TQEKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNI 341
            ET+HS DQ E E  E NI E D+ E    +++N+++LSKV E D S DPNESE + S INEV++ E KQQE VN +E+S+V EKDNSY+PN+    E  I  +D+ + NI
Sbjct: 2417 ETNHSVDQNEGELDEQNIIEIDKSEKNISDQVNVDELSKVDEIDHSADPNESESANSNINEVNKKETKQQELVNSKELSEVYEKDNSYNPNKKEFVEPNIKNVDEVEKNI 2526

HSP 13 Score: 81.2629 bits (199), Expect = 6.885e-19
Identity = 56/114 (49.12%), Postives = 77/114 (67.54%), Query Frame = 3 
Query:   21 DHSADQIEREPVESNINEADEEEITQ-EKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            DHS +  E+   E    E D+ E TQ + +  E+ SK+ EK  S DPNESEP+ES INEVD+ E KQQ++++ EEVS V++ D+S D NES LAE  I+E+D G+ NI++  NL
Sbjct: 1734 DHSVNLNEKTLTEPITKEIDKNEQTQTDHVISEEKSKLDEKYYSADPNESEPAESNINEVDKAERKQQKKIDLEEVSNVDKTDHSADSNESKLAEQNIIEVDVGERNISEQDNL 1847

HSP 14 Score: 78.5666 bits (192), Expect = 5.551e-18
Identity = 58/116 (50.00%), Postives = 74/116 (63.79%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEAD-EEEITQEKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            +TDHSAD  E +  E NI E D  E    E+ NLE++S V+E D S D NE+EP+ES IN+VDE E   QEQ+  EEVSKV EKD S DPNES   E+ I ++D+ +      +NL
Sbjct: 1814 KTDHSADSNESKLAEQNIIEVDVGERNISEQDNLEEVSNVFETDPSVDLNENEPTESNINKVDE-EETTQEQLISEEVSKVGEKDVSVDPNESDFVEHNIKKVDEDEKKQHKQINL 1928

HSP 15 Score: 78.5666 bits (192), Expect = 6.298e-18
Identity = 57/107 (53.27%), Postives = 70/107 (65.42%), Query Frame = 3 
Query:   18 TDHSADQIEREPVESNINEAD-EEEITQEKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKN 335
            TDHS +  E E  E NI E D ++    EK+NL+++SKV E D   DPNESE +ES IN+VDEGE KQQEQVN EEVS V E +NS   NE+ L E    E+D  +N
Sbjct: 2213 TDHSGEPNEHELAEQNIIEVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNINDVDEGETKQQEQVNFEEVSNVFELENSVILNENTLTEPIKKEVDNVEN 2319

HSP 16 Score: 78.1814 bits (191), Expect = 8.345e-18
Identity = 58/116 (50.00%), Postives = 75/116 (64.66%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEA-DEEEITQEKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            E D+S DQ E    E  + E  + E I  +++N E+ SK+ E D S D NESE +ES I+EVDEGE KQ EQVN EEVS V+  D+S +PNE  LAE  I+ +D  K NI++ VNL
Sbjct: 1404 ERDYSVDQNEITLTEPIMKEVYNVENIQIQRVNSEENSKLDETDHSVDLNESELAESNISEVDEGETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIGVDVDKRNISEKVNL 1519

HSP 17 Score: 76.6406 bits (187), Expect = 2.451e-17
Identity = 56/107 (52.34%), Postives = 69/107 (64.49%), Query Frame = 3 
Query:   18 TDHSADQIEREPVESNINEAD-EEEITQEKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKN 335
            TDHS +  E E  E NI   D ++    EK+NL+++SKV E D   DPNESE +ES IN+VDEGE KQQEQVN EEVS V E +NS   NE+ L E    E+D  +N
Sbjct: 1487 TDHSGEPNEHELAEQNIIGVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNINDVDEGETKQQEQVNFEEVSNVYELENSVILNENTLTESIKKEVDNVEN 1593

HSP 18 Score: 71.2478 bits (173), Expect = 1.825e-15
Identity = 55/116 (47.41%), Postives = 71/116 (61.21%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEEITQ-EKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            ETDHS D  E E  ESNI+E DE E  Q E++N E++S V   D SG+PNE E +E  I  VD  +    E+VN +EVSKV+E D   DPNES  AE  I ++D+G+    + VN 
Sbjct: 1445 ETDHSVDLNESELAESNISEVDEGETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIGVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNINDVDEGETKQQEQVNF 1560

HSP 19 Score: 69.707 bits (169), Expect = 6.967e-15
Identity = 52/104 (50.00%), Postives = 69/104 (66.35%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEEITQEKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQ 326
            ETDHS DQ + E  +  I E DE     EK  L+ ++ V E D S + N+SEPSES INEVDE + KQQ++V+ + VSKV++KD S + NES LAE  I E+D+
Sbjct: 2094 ETDHSDDQNDGELAKQIIIEIDE----VEKNILDQVNVVDETDHSANSNKSEPSESKINEVDEADWKQQKKVDLDGVSKVDKKDRSINLNESELAESNISEVDE 2193

HSP 20 Score: 68.9366 bits (167), Expect = 1.223e-14
Identity = 40/62 (64.52%), Postives = 48/62 (77.42%), Query Frame = 3 
Query:  105 LNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNES 290
            +N++++SKV E D + + NESEP ES I EVD GEPKQ+EQVN EEVSKVNE D S DPN S
Sbjct: 2813 VNVDEVSKVEETDRTANLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGS 2874

HSP 21 Score: 68.1662 bits (165), Expect = 2.169e-14
Identity = 52/116 (44.83%), Postives = 72/116 (62.07%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEE-ITQEKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            +TDHS D  + +  E NINE D  E   QE++N +++SKV  KD S D NESE  E  + E+DE E    E+ N +E+  VNE D+S D N+  LA+  I+EID+ + NI D VN+
Sbjct: 2012 KTDHSVDPNKIKLAEFNINEVDNGEPKQQEQVNSDEVSKVDVKDKSVDTNESELCEPNVIEIDEVEKNISEKSNVKELLNVNETDHSDDQNDGELAKQIIIEIDEVEKNILDQVNV 2127

HSP 22 Score: 67.3958 bits (163), Expect = 5.297e-14
Identity = 51/116 (43.97%), Postives = 72/116 (62.07%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEEITQ-EKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            ETDHS    + EP ESNIN+ DEEE  Q E++N +++  V  KD S +P+ESE  E    E+ E +    E+ N +E+ KV E ++S D NE  L E  I+EID+ + NI+D VN+
Sbjct: 2335 ETDHSIFPNQSEPAESNINKVDEEETKQQEQVNSDEVINVDVKDKSVNPDESELGEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQNIIEIDKSEKNISDQVNV 2450

HSP 23 Score: 65.855 bits (159), Expect = 1.665e-13
Identity = 52/114 (45.61%), Postives = 69/114 (60.53%), Query Frame = 3 
Query:   21 DHSADQIEREPVESNINEADEEEITQ-EKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            D S +  E E  ESNI+E DE E  Q E++N E++S V   D SG+PNE E +E  I EVD  +    E+VN +EVSKV+E D   DPNES  AE  I ++D+G+    + VN 
Sbjct: 2173 DRSINLNESELAESNISEVDERETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIEVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNINDVDEGETKQQEQVNF 2286

HSP 24 Score: 65.0846 bits (157), Expect = 2.867e-13
Identity = 58/157 (36.94%), Postives = 76/157 (48.41%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEEITQ-EKLNLEDLSKVYE-----------------------------------------KDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            E D   D  E E  ESNIN+ DE E  Q E++N E++S V+E                                          D S  PN+SEP+ES IN+VDE E KQQEQVN +EV  V+ KD S +P+ES L E   +EI +   NI++  N+
Sbjct: 2253 EIDQFVDPNESESAESNINDVDEGETKQQEQVNFEEVSNVFELENSVILNENTLTEPIKKEVDNVENIQNVHANSEEESKVDETDHSIFPNQSEPAESNINKVDEEETKQQEQVNSDEVINVDVKDKSVNPDESELGEPNCIEIVEVDKNISEESNV 2409

HSP 25 Score: 64.3142 bits (155), Expect = 5.937e-13
Identity = 44/100 (44.00%), Postives = 67/100 (67.00%), Query Frame = 3 
Query:   21 DHSADQIEREPVESNINEADEEEITQEKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEI 320
            D+S D  E   +E+ IN+ DEE I Q+++NLE++ KV E ++  +PN SE ++  I +V+E E K+QEQ+N EEV+ V+E+D S D NE  L E  + E+
Sbjct: 1326 DNSVDPYESAHIEAKINKIDEETIKQKQVNLEEVPKVDETEEFINPNRSELADHNIIKVEE-ETKKQEQINLEEVTNVDERDYSVDQNEITLTEPIMKEV 1424

HSP 26 Score: 63.1586 bits (152), Expect = 1.381e-12
Identity = 50/102 (49.02%), Postives = 64/102 (62.75%), Query Frame = 3 
Query:   24 HSADQIEREPVESNINEADEEEITQEK-LNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQ 326
            +SAD  E EP ESNINE D+ E  Q+K ++LE++S V + D S D NES+ +E  I EVD GE    EQ N EEVS V E D S D NE+   E  I ++D+
Sbjct: 1776 YSADPNESEPAESNINEVDKAERKQQKKIDLEEVSNVDKTDHSADSNESKLAEQNIIEVDVGERNISEQDNLEEVSNVFETDPSVDLNENEPTESNINKVDE 1877

HSP 27 Score: 60.8474 bits (146), Expect = 8.075e-12
Identity = 50/116 (43.10%), Postives = 70/116 (60.34%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEEITQE-KLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            ETDHS    + EP ESNIN+ DEEE  Q+ ++N E + +V EK +S DPN  E  +  +  VDE E    E+ N + +S V+EK++S D  +S LAE  I  +D+ + NIT   NL
Sbjct: 1609 ETDHSIFPNQSEPAESNINKVDEEETKQQDQVNSEKVIEVVEKYNSDDPNYGEFIDPNMKNVDEEEKNIIEESNVKSLSNVDEKEHSTDQIKSELAEPNIRNVDEEERNITADANL 1724

HSP 28 Score: 58.9214 bits (141), Expect = 4.766e-11
Identity = 56/157 (35.67%), Postives = 74/157 (47.13%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEEITQ-EKLNLEDLSKVY-----------------------------------------EKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            E D   D  E E  ESNIN+ DE E  Q E++N E++S VY                                         E D S  PN+SEP+ES IN+VDE E KQQ+QVN E+V +V EK NS DPN     +  +  +D+ + NI +  N+
Sbjct: 1527 EIDQFVDPNESESAESNINDVDEGETKQQEQVNFEEVSNVYELENSVILNENTLTESIKKEVDNVENIQNVHANSEEESKVDETDHSIFPNQSEPAESNINKVDEEETKQQDQVNSEKVIEVVEKYNSDDPNYGEFIDPNMKNVDEEEKNIIEESNV 1683

HSP 29 Score: 58.5362 bits (140), Expect = 6.628e-11
Identity = 45/102 (44.12%), Postives = 57/102 (55.88%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEEITQ-EKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVE 317
            E DHS +  ER     N+ E D+ +  Q E  NLE++SKV E   S +PNESE  ES I EVDEGE  QQ+QVNP E + +    N  D  E    E+  +E
Sbjct: 3705 EIDHSVNSNERTLTGDNVIEVDKGQKNQHEPANLEEMSKVDEIHHSANPNESELDESNIYEVDEGETNQQKQVNPNESTPIECNTNEVDGGEKYQTEHVNLE 3806

HSP 30 Score: 58.151 bits (139), Expect = 7.893e-11
Identity = 48/116 (41.38%), Postives = 66/116 (56.90%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEEITQEK--LNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVN 356
            E D S D  + E  E NIN  DEEE  Q++   N E+ SKV+EKD+S DPNE+E  E +I  + EGE    E+ N +E++KV  K +S+D           +E +  + NITD VN
Sbjct: 3484 EKDDSFDPNKSELAELNINGVDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNISEESNVKELTKVFGKQHSFDQ----------IEGNSEERNITDDVN 3589

HSP 31 Score: 57.7658 bits (138), Expect = 1.152e-10
Identity = 45/114 (39.47%), Postives = 65/114 (57.02%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEEITQEKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVN 356
            +T+HS +Q++ +  E NI +           NL DLS+V E D S + NE   +   + EVD+G+  Q E  N EE+SKV+E  +S +PNES L E  I E+D+G+ N    VN
Sbjct: 3674 KTEHSVNQMKCKIEERNITD---------DANLRDLSQVDEIDHSVNSNERTLTGDNVIEVDKGQKNQHEPANLEEMSKVDEIHHSANPNESELDESNIYEVDEGETNQQKQVN 3778

HSP 32 Score: 56.9954 bits (136), Expect = 2.022e-10
Identity = 48/107 (44.86%), Postives = 63/107 (58.88%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEE--ITQEKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQG 329
            E +HS DQI+ E  E NI   DEEE  IT +  NL++ SKV + D S + NE   +E    E+D+ E  Q + V  EE SK++EK  S DPNES  AE  I E+D+ 
Sbjct: 1691 EKEHSTDQIKSELAEPNIRNVDEEERNITADA-NLKEESKVDKIDHSVNLNEKTLTEPITKEIDKNEQTQTDHVISEEKSKLDEKYYSADPNESEPAESNINEVDKA 1796

HSP 33 Score: 53.5286 bits (127), Expect = 2.967e-9
Identity = 46/101 (45.54%), Postives = 61/101 (60.40%), Query Frame = 3 
Query:   84 EEITQEKLNLEDLSKVYEKDD---------SGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            E IT+E  N+E++   Y K +         S D  E E +ES I+EVDE E   QE+ N +EVSKV+E D+S DPNES  +   I EI QG+ NI+D VN+
Sbjct: 2595 EPITKEVDNIENIQTDYVKSEELKADETEYSVDQIERESAESNIDEVDEEEI-TQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNV 2694

HSP 34 Score: 52.7582 bits (125), Expect = 6.020e-9
Identity = 33/76 (43.42%), Postives = 53/76 (69.74%), Query Frame = 3 
Query:   99 EKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQ 326
            E  NL  ++K+ +KD+S DP ES   E+ IN++DE E  +Q+QVN EEV KV+E +   +PN S LA++ I+++++
Sbjct: 1312 ETFNLYVMTKMDQKDNSVDPYESAHIEAKINKIDE-ETIKQKQVNLEEVPKVDETEEFINPNRSELADHNIIKVEE 1386

HSP 35 Score: 49.6766 bits (117), Expect = 7.192e-8
Identity = 46/107 (42.99%), Postives = 60/107 (56.07%), Query Frame = 3 
Query:   24 HSADQIEREPVESNINEADEEEITQEKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNIT 344
            HS DQIE    E NI +          +N +D+S V E D + D NESEP+ES  NEVDE E   +E+      SKV+EKDNS DP ++ L E  I   + G+ NI+
Sbjct: 3570 HSFDQIEGNSEERNITD---------DVNYKDVSHVDETDHADDSNESEPAESNFNEVDEEEGNSEEE------SKVDEKDNSDDPYKNELVEPLIKNFNVGEKNIS 3661

HSP 36 Score: 44.2838 bits (103), Expect = 5.085e-6
Identity = 50/146 (34.25%), Postives = 69/146 (47.26%), Query Frame = 3 
Query:   15 ETDHSADQIEREPVESNINEADEEEITQEKLNLEDLSKVYEKDDSGDPNESEPSESYINEVDEGEP---------------KQQEQVNP----------------EEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNL 359
            ETDH+ D  E EP ESN NE DEEE   E+      SKV EKD+S DP ++E  E  I   + GE                K +  VN                  ++S+V+E D+S + NE  L    ++E+D+G+ N  +P NL
Sbjct: 3598 ETDHADDSNESEPAESNFNEVDEEEGNSEEE-----SKVDEKDNSDDPYKNELVEPLIKNFNVGEKNISGESCLKDLLKVDKTEHSVNQMKCKIEERNITDDANLRDLSQVDEIDHSVNSNERTLTGDNVIEVDKGQKNQHEPANL 3738
The following BLAST results are available for this feature:
BLAST of SMED30011999 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011999 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011999 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011999 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011999 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011999 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011999 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011999 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011999 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011999 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011999 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011999 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011999 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011999 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000064314.11.769e-6896.64SMESG000064314.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011999 ID=SMED30011999|Name=SMED30011999|organism=Schmidtea mediterranea sexual|type=transcript|length=360bp
GTGATCTAAATGAAGAAACAGATCATTCTGCTGATCAAATTGAACGTGAA
CCTGTTGAGTCTAATATCAATGAAGCCGACGAGGAAGAAATAACGCAAGA
GAAACTTAATTTGGAAGATTTATCAAAAGTTTATGAAAAAGATGACTCTG
GTGATCCAAATGAAAGTGAGCCTTCTGAGTCTTATATCAATGAGGTTGAT
GAGGGCGAACCGAAACAACAAGAACAGGTTAATCCTGAAGAGGTATCAAA
GGTTAATGAAAAAGATAATTCCTATGATCCTAATGAAAGTGGACTTGCTG
AGTATTATATCGTCGAAATCGATCAAGGTAAAAATAATATAACAGATCCA
GTTAATTTGA
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protein sequence of SMED30011999-orf-1

>SMED30011999-orf-1 ID=SMED30011999-orf-1|Name=SMED30011999-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=119bp
DLNEETDHSADQIEREPVESNINEADEEEITQEKLNLEDLSKVYEKDDSG
DPNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAE
YYIVEIDQGKNNITDPVNL
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0002089reproductive organ
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..53
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..96
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 64..90