Metalloendopeptidase

Overview
NameMetalloendopeptidase
Smed IDSMED30011992
Length (bp)691
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Metalloendopeptidase (SMED30011992) t-SNE clustered cells

Violin plots show distribution of expression levels for Metalloendopeptidase (SMED30011992) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Metalloendopeptidase (SMED30011992) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Metalloendopeptidase (SMED30011992) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30011992

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30011992h1SMcG0010099 dd_Smed_v4_24374_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30011992h1SMcG0010099 dd_Smed_v4_24374_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30011992h1SMcG0010099 dd_Smed_v4_24374_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Metalloendopeptidase vs. Ensembl Fly
Match: Cubn (gene:FBgn0052702 transcript:FBtr0273437)

HSP 1 Score: 51.2174 bits (121), Expect = 3.525e-7
Identity = 33/116 (28.45%), Postives = 54/116 (46.55%), Query Frame = 1 
Query:  175 ELRSPSYPKTNTYDIICRWLIXXXXXXXXXXVTHVIQIKLNDIDILNAPSCSKGYLEIRDGGRVWDYSQAKLCGTD---GVDSYISTNRYFLVTFVSGRDSMNPKRGFSLTYKCIA 513
            EL SP YP   +  + C W +  T       + H+++I++ + ++  +P+CS  YLE+R+GG        + CG D    +  +    R  L T     DS    RGF L ++  A
Sbjct: 1110 ELTSPEYPANYSAGLDCDWHLTGT-------IDHLLEIQVENFELEQSPNCSADYLEVRNGGGTDSPLIGRFCGRDIPARIPGFSHEMRLILHT-----DSAINGRGFRLRWRIFA 1213
[Close]
BLAST of Metalloendopeptidase vs. Ensembl Xenopus
Match: slain1 (SLAIN motif family member 1 [Source:Xenbase;Acc:XB-GENE-5793178])

HSP 1 Score: 48.521 bits (114), Expect = 3.896e-6
Identity = 40/151 (26.49%), Postives = 67/151 (44.37%), Query Frame = 1 
Query:  106 DDEKMRQNNCAFYKLLNASENETELRSPSYPKTNTYDIICRWLIXXXXXXXXXXVTHVIQIKLNDIDILNAPSCSKGYLEIRDGGRVWDYS-QAKLCGTDGVDSYISTNRYFLVTFVSGRDSMNPKRGFSLTYKC--IAKDKYFDQPVNFK 549
            D+E   Q N     LLN+ +    L+SP++P      IICRWL++            +I+++ ++  + +   C   Y+EI D   +   S   + CG+    +  S+     V FV+  D +    GFS TY+   I + KY   P   +
Sbjct:   86 DEENCNQTNKDCGGLLNSMQGS--LQSPNHPGFYPGKIICRWLLSVQDGL-------IIKLQFHNFSLESDLRCRFDYVEIHDSAGLGTPSLMGRFCGSQVPPTLTSSGAQMTVLFVA--DEVTSGLGFSATYEAINITESKYECNPKELR 225
[Close]
BLAST of Metalloendopeptidase vs. Ensembl Xenopus
Match: wdr81 (WD repeat domain 81 [Source:Xenbase;Acc:XB-GENE-6258221])

HSP 1 Score: 48.521 bits (114), Expect = 4.620e-6
Identity = 35/137 (25.55%), Postives = 54/137 (39.42%), Query Frame = 1 
Query:  106 DDEKMRQNNCAFYKLLNAS--ENETELRSPSYPKTNTYDIICRWLIXXXXXXXXXXVTHVIQIKLNDIDILNAPSCSKGYLEIRDGGRVWDYSQAKLCGTDGVDSYISTNRYFLVTFVSGRDSMNPKRGFSLTYKCI 510
            D    R +  AFY++           + +P YP     +  C W+I             V+ +  N  DIL   +C   ++EIRDG         K CG +      ST R   + F +  DS     GFS++Y+ +
Sbjct:  782 DASVTRSSFKAFYQVACGGFLTGAGVIHTPHYPDAYFRERTCEWIITQPEG-------EVVSLTFNSFDILAGTNCGSNFVEIRDGSSSDSPLIGKYCGPEMPVPAQSTQRALYIRFTT--DSSATNHGFSISYQSL 909
[Close]
BLAST of Metalloendopeptidase vs. Ensembl Xenopus
Match: mtx3 (metaxin 3 [Source:Xenbase;Acc:XB-GENE-994127])

HSP 1 Score: 47.7506 bits (112), Expect = 6.568e-6
Identity = 37/136 (27.21%), Postives = 62/136 (45.59%), Query Frame = 1 
Query:  106 DDEKMRQNNCAFYKLLNASENETELRSPSYPKTNTYDIICRWLIXXXXXXXXXXVTHVIQIKLNDIDILNAPSCSKGYLEIRDGGRVWDYS-QAKLCGTDGVDSYISTNRYFLVTFVSGRDSMNPKRGFSLTYKCI 510
            D+E   Q N     LLN+ +    L+SP++P      IICRWL++            +I+++ ++  + +   C   Y+EI D   +   S   + CG+    +  S+     V FV+  D +    GFS TY+ I
Sbjct:  272 DEENCNQTNKDCGGLLNSMQGS--LQSPNHPGFYPGKIICRWLLSVQDGL-------IIKLQFHNFSLESDLRCRFDYVEIHDSAGLGTPSLMGRFCGSQVPPTLTSSGAQMTVLFVA--DEVTSGLGFSATYEAI 396
[Close]
BLAST of Metalloendopeptidase vs. Ensembl Xenopus
Match: nol10 (nucleolar protein 10 [Source:Xenbase;Acc:XB-GENE-5799993])

HSP 1 Score: 47.3654 bits (111), Expect = 9.574e-6
Identity = 35/127 (27.56%), Postives = 57/127 (44.88%), Query Frame = 1 
Query:  160 SENETELRSPSYPKTNTYDIICRWLIXXXXXXXXXXVTHVIQIKLNDIDILNAPSCSKGYLEIRDGGRVWDYS-QAKLCGTDGVDSYISTNRYFLVTFVSGRDSMNPKRGFSLTYKCIAKDKYFDQP 537
            S++   L S +YP +  ++  C WLI +         +  I ++ +  D+ ++  C+  YL+I DGG   D     + CGT  + S +ST    L+ FVS  D      GF  +Y  +     F  P
Sbjct:  235 SDSTGSLMSSNYPGSYPHNTKCSWLIRAP--------SDQIFLQFSAFDVQSSAGCASDYLKIYDGGSRSDPVLLDRACGTGQLPSVVSTGNLLLLDFVS--DQSIGGNGFKASYNTVQCGSSFFNP 351
[Close]
BLAST of Metalloendopeptidase vs. UniProt/SwissProt
Match: sp|F1RWC3|CUBN_PIG (Cubilin OS=Sus scrofa OX=9823 GN=CUBN PE=1 SV=3)

HSP 1 Score: 55.4546 bits (132), Expect = 1.434e-7
Identity = 33/110 (30.00%), Postives = 57/110 (51.82%), Query Frame = 1 
Query:  178 LRSPSYPKTN-TYDIICRWLIXXXXXXXXXXVTHVIQIKLNDIDILNAPSCSKGYLEIRDGGRVWDYSQAKLCGTDGVDSYISTNRYFLVTFVSGRDSMNPKRGFSLTYK 504
            ++SPSY  +N   D+ C + +             VIQ+K ND D++ +  CS+ YLEI DG  + D    K CG+    +  S+N    + F +  DS++  RG+ ++++
Sbjct: 3050 IKSPSYSYSNYPNDMHCLYTVTVRD-------DRVIQLKFNDFDLVPSTFCSQDYLEIYDGSNISDPLLGKFCGSTLPPNVKSSNNTMFLVFKT--DSVHTARGWKISFR 3150
[Close]
BLAST of Metalloendopeptidase vs. UniProt/SwissProt
Match: sp|Q9W332|CUBN_DROME (Cubilin homolog OS=Drosophila melanogaster OX=7227 GN=Cubn PE=2 SV=3)

HSP 1 Score: 51.2174 bits (121), Expect = 3.534e-6
Identity = 33/116 (28.45%), Postives = 54/116 (46.55%), Query Frame = 1 
Query:  175 ELRSPSYPKTNTYDIICRWLIXXXXXXXXXXVTHVIQIKLNDIDILNAPSCSKGYLEIRDGGRVWDYSQAKLCGTD---GVDSYISTNRYFLVTFVSGRDSMNPKRGFSLTYKCIA 513
            EL SP YP   +  + C W +  T       + H+++I++ + ++  +P+CS  YLE+R+GG        + CG D    +  +    R  L T     DS    RGF L ++  A
Sbjct: 1110 ELTSPEYPANYSAGLDCDWHLTGT-------IDHLLEIQVENFELEQSPNCSADYLEVRNGGGTDSPLIGRFCGRDIPARIPGFSHEMRLILHT-----DSAINGRGFRLRWRIFA 1213
[Close]
BLAST of Metalloendopeptidase vs. UniProt/SwissProt
Match: sp|Q9TU53|CUBN_CANLF (Cubilin OS=Canis lupus familiaris OX=9615 GN=CUBN PE=1 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 7.499e-6
Identity = 31/110 (28.18%), Postives = 56/110 (50.91%), Query Frame = 1 
Query:  178 LRSPSYPKTN-TYDIICRWLIXXXXXXXXXXVTHVIQIKLNDIDILNAPSCSKGYLEIRDGGRVWDYSQAKLCGTDGVDSYISTNRYFLVTFVSGRDSMNPKRGFSLTYK 504
            ++SP+Y  ++   +I C + I             V+Q+K +D D++ +  CS+ YL I DG  + D    K CG++   +  S+N   L+ F +  DS    RG+ +T++
Sbjct: 3045 IKSPAYSYSDYPNNIYCLYTIVGRD-------DRVVQLKFSDFDVVPSTFCSQDYLAIYDGSNISDPLLGKFCGSNLPPNIKSSNHSMLLVFKT--DSFQTARGWKITFQ 3145
[Close]
BLAST of Metalloendopeptidase vs. TrEMBL
Match: A0A1I8IF63 (CUB domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.466e-11
Identity = 45/156 (28.85%), Postives = 75/156 (48.08%), Query Frame = 1 
Query:  109 DEKMRQNNCAFYKLLNASENETELRSPSYPKTNTYDIICRWLIXXXXXXXXXXVTHVIQIKLN--DIDILNAPSCSKGYLEIRDGGRVWDYSQAKLCGTDGVDS-----YISTNRYFLVTFVSGRDSMNPK-----RGFSLTYKCIAKDKYFDQPV 540
            D    Q +CA Y    A  +   +RSP+YP     + +CRW++++   S         +++    D +I ++PSC++ ++E++DG R+W +S  + CG   V         ST  + LVTF +  D  N +     RGF   Y+C+   K    PV
Sbjct:   41 DATATQGSCADYYQAFADTSYQYIRSPNYPGPYADNQVCRWVLSACRCSNPLRPEAKFRVEFEVLDFEIQDSPSCTQDFVEVKDGKRLWAHSFGQYCGNSCVKGNSNNLTQSTGLHLLVTFQT--DVFNARQPQKYRGFQARYRCVELGKLLRSPV 194
[Close]
BLAST of Metalloendopeptidase vs. TrEMBL
Match: A0A2I9LPU7 (Metalloendopeptidase OS=Centruroides hentzi OX=88313 PE=4 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 3.867e-7
Identity = 39/113 (34.51%), Postives = 55/113 (48.67%), Query Frame = 1 
Query:  178 LRSPSYPKTNTYDIICRWLIXXXXXXXXXXVTHVIQIKLN--DIDILNAPSCSKGYLEIRDGGRVWDYSQAKLCGTDGVDSYISTNRYFLVTFVSGRDSMNPKRGFSLTYKCI 510
            + SP  P ++   + C W I           TH  +I  N  D+DI   P C   YLEIRDG  V      +LCG++ + S+IST+   LVT+ +     N  RGFS  Y+ +
Sbjct:  349 ISSPQNPPSDE-GVHCEWRIT---------ATHGERIVFNITDLDIFETPKCDTDYLEIRDGYWVKSPMLGRLCGSNTIQSFISTSHRLLVTYKTSSRQGN-HRGFSANYEAV 450
[Close]
BLAST of Metalloendopeptidase vs. TrEMBL
Match: A0A482V1R9 (Metalloendopeptidase (Fragment) OS=Asbolus verrucosus OX=1661398 GN=BDFB_005888 PE=4 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 9.880e-7
Identity = 48/144 (33.33%), Postives = 65/144 (45.14%), Query Frame = 1 
Query:  166 NETELRSPSYPKTNTYDI--ICRWLIXXXXXXXXXXVTHVIQIKLN--DIDILNAPSCSKGYLEIRDGGRVWDYSQ--AKLCGTDGVDSYISTNRYFLVTFVSGRDSMNPKRGFSLTYKCI-AKDKYFDQPVNFKIINYVLIYM 576
            N     SPSY   N  +    C W I           TH  +I LN  ++DI  +P CS  ++EIRDG   W  S    K CGT  +DS  ST    LV++V+   + N  RGF+  Y+ I   D + D   + +  NY   Y 
Sbjct:  365 NSATFSSPSYINQNGPEGGEKCEWRIT---------ATHGEKIVLNITELDIEKSPDCSTDHIEIRDG--YWHKSPLLGKFCGTGKLDSITSTGSRMLVSYVA--KNPNGHRGFTANYEAICGGDLFIDSEGHLESPNYPEEYQ 495
[Close]
BLAST of Metalloendopeptidase vs. TrEMBL
Match: A0A3M6V1S3 (Uncharacterized protein OS=Pocillopora damicornis OX=46731 GN=pdam_00001153 PE=4 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.055e-6
Identity = 38/140 (27.14%), Postives = 62/140 (44.29%), Query Frame = 1 
Query:  178 LRSPSYPKTNTYDIICRWLIXXXXXXXXXXVTHVIQIKLNDIDILN---APSCSKGYLEIRDGGRVWDYSQAKLCGTDGVDSYISTNRYFLVTFVSGRDSMNPKRGFSLTYKCIAKDKYFDQPVNFKIINYVLIYMSVGI 588
              SP YPK     + C W+I            H++++  +D ++ +   +  C+  ++E+RDG         + CG D   + IST RY  V F +  DS   ++GF  TYK I    Y +    + I     I  +VG+
Sbjct:  332 FSSPGYPKEYPNSVTCTWVIEVPE-------NHLVELTFDDFELEDCFISSICTCDHVEVRDGKDKSASELDEFCGDDKPSTLISTGRYMWVEFEA--DSRTNRKGFHATYKAIKTGMYCNTAFGWNISAIGCIAATVGV 462
[Close]
BLAST of Metalloendopeptidase vs. TrEMBL
Match: A0A139WL18 (Metalloendopeptidase OS=Tribolium castaneum OX=7070 GN=TcasGA2_TC034615 PE=4 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 2.599e-6
Identity = 48/144 (33.33%), Postives = 62/144 (43.06%), Query Frame = 1 
Query:  166 NETELRSPSYPKTNTYDI--ICRWLIXXXXXXXXXXVTHVIQIKLN--DIDILNAPSCSKGYLEIRDGGRVWDYSQ--AKLCGTDGVDSYISTNRYFLVTFVSGRDSMNPKRGFSLTYKCI-AKDKYFDQPVNFKIINYVLIYM 576
            N     SPSY   N  +    C W I           TH  +I LN  D+DI  +P C   Y+EIRDG   W  S    K CGT  +DS  ST    L+ +V+   + N  RGF   Y+ I   + Y D   + +  NY   Y 
Sbjct:  413 NSATFSSPSYLNHNAPEDGEKCEWRIT---------ATHGEKIVLNITDLDIEKSPDCGTDYIEIRDG--YWHKSPLLGKFCGTGKLDSITSTGSRMLINYVA--KNPNGHRGFVANYEAICGGELYIDSEGHLESPNYPEEYQ 543
[Close]
BLAST of Metalloendopeptidase vs. Ensembl Nematostella
Match: EDO43187 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZ83])

HSP 1 Score: 46.595 bits (109), Expect = 6.387e-7
Identity = 30/116 (25.86%), Postives = 54/116 (46.55%), Query Frame = 1 
Query:  175 ELRSPSYPKTNTYDIICRWLIXXXXXXXXXXVTHVIQIKLNDIDILNAPSCSKGYLEIRDGGRVWDYSQAKLCGTDGVDSYISTNRYFLVTFVSGRDSMNPKRGFSLTYKCIAKDK 522
            E+ SP+YP      + C W I+         V +VI++     D+ + P C++ +LE+ DGG+    S  + CG     S ++      + F S   + + ++GF L Y  +  +K
Sbjct:    7 EIFSPNYPGYYPGSMSCTWRISVP-------VGNVIRLTFIMFDLEDDPLCARDFLELMDGGQDSRMSIGRFCGNRYPLSIMTATSRLKIVFTS--TAFSGRQGFRLRYDAVVGNK 113
[Close]
BLAST of Metalloendopeptidase vs. Ensembl Nematostella
Match: EDO39003 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBC0])

HSP 1 Score: 46.595 bits (109), Expect = 4.893e-6
Identity = 34/121 (28.10%), Postives = 56/121 (46.28%), Query Frame = 1 
Query:  175 ELRSPSYPKTNTYDIICRWLIXXXXXXXXXXVTHVIQIKLNDIDILNAPSCSKGYLEIRDGGRVWDYSQAKLCGTDGVDSYISTNRYFLVTFVSGRDSMNPKRGFSLTYKCIAKDKYFDQP 537
            E+ SP+YP T      C W+I+     +       +Q+   + ++ N   C   Y++++DGG V   + A+LCG     S ++TN    ++ VS  D      GF  TY    KD+  + P
Sbjct:  179 EITSPNYPSTYPAQASCSWVIDRGAGFS-------VQLIFREFELENHTRCLYDYVKVQDGGLVGSPTIARLCGKHENFS-LTTNGPMRISLVS--DKHQAYSGFRATYSTSDKDECQNNP 289
[Close]
BLAST of Metalloendopeptidase vs. Ensembl Nematostella
Match: EDO49823 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG22])

HSP 1 Score: 43.5134 bits (101), Expect = 7.036e-6
Identity = 27/116 (23.28%), Postives = 52/116 (44.83%), Query Frame = 1 
Query:  163 ENETELRSPSYPKTNTYDIICRWLIXXXXXXXXXXVTHVIQIKLNDIDILNAPSCSKGYLEIRDGGRVWDYSQ--AKLCGTDGVDSYISTNRYFLVTFVSGRDSMNPKRGFSLTYK 504
            E+   + SP YP     +++C+W +          + + +++ + D  +     C   Y+ + DG    D ++   + CGT G    +ST R  ++ F S RD +   +GF   +K
Sbjct:    6 ESGGNITSPFYPSNYPTNVLCKWTLVVP-------INYTVELNIADFQLEKDKKCFYDYIRVLDGNTT-DAAELLPRTCGTLGSRKIVSTGRILMLEFHSDRDVI--AKGFRAEWK 111
[Close]
BLAST of Metalloendopeptidase vs. Ensembl Medaka
Match: cubn (cubilin [Source:NCBI gene;Acc:105353827])

HSP 1 Score: 48.1358 bits (113), Expect = 4.048e-6
Identity = 32/109 (29.36%), Postives = 48/109 (44.04%), Query Frame = 1 
Query:  175 ELRSPSYPKTNTYDIICRWLIXXXXXXXXXXVTHVIQIKLNDIDILNAPSCSKGYLEIRDGGRVWDYSQAKLCGTDGVDSYISTNRYFLVTFVSGRDSMNPKRGFSLTY 501
            E+ SP YP +   ++ C W+I+           H + +   D+DI    +C+  Y+ I DG  +     A +CG+    S  ST     V F S  DS    RGFS  +
Sbjct: 1510 EIHSPLYPNSYPANVDCSWVISVDH-------NHRVFLNFTDLDIELHTNCTWDYVAIHDGPSISSPLLAHICGSSLPTSITSTQNTIYVRFRS--DSSRDHRGFSARF 1609
[Close]
BLAST of Metalloendopeptidase vs. Planmine SMEST
Match: SMESG000064589.1 (SMESG000064589.1)

HSP 1 Score: 275.404 bits (703), Expect = 3.700e-93
Identity = 140/142 (98.59%), Postives = 142/142 (100.00%), Query Frame = 1 
Query:  118 MRQNNCAFYKLLNASENETELRSPSYPKTNTYDIICRWLIXXXXXXXXXXVTHVIQIKLNDIDILNAPSCSKGYLEIRDGGRVWDYSQAKLCGTDGVDSYISTNRYFLVTFVSGRDSMNPKRGFSLTYKCIAKDKYFDQPVN 543
            MRQNNCAFYKLLNASENETELRSPSYPKTNTYDIICRWLINSTCSSTNSSVTHVIQIKLNDIDILNAPSCSKGYLEIRDGGRVWDYSQAKLCGTDGVDSYISTNRYFLVTFVSGRDSMNPKRGFSLTYKCIAKDKYFDQP++
Sbjct:    1 MRQNNCAFYKLLNASENETELRSPSYPKTNTYDIICRWLINSTCSSTNSSVTHVIQIKLNDIDILNAPSCSKGYLEIRDGGRVWDYSQAKLCGTDGVDSYISTNRYFLVTFVSGRDSMNPKRGFSLTYKCIAKDKYFDQPIS 142
[Close]
BLAST of Metalloendopeptidase vs. Planmine SMEST
Match: SMESG000005882.1 (SMESG000005882.1)

HSP 1 Score: 51.2174 bits (121), Expect = 2.765e-7
Identity = 33/116 (28.45%), Postives = 53/116 (45.69%), Query Frame = 1 
Query:  175 ELRSPSYPKTNTYDIICRWLIXXXXXXXXXXVTHVIQIKLNDIDILNAPSCSKGYLEIRDGGRVWDYSQAKLCGTDGVDSYISTNRYFLVTFVSGRDSMNPKRGFSLTYKCIAKDK 522
            E++SP+YP T      C W I +T         H+IQ+  +D +I +   C+   L + DG  + +    + CG+       S +   L+TF S  D    +RGF +T+  I   K
Sbjct:  808 EIKSPNYPVTYPSKSNCEWKIITT-------PGHIIQLTFSDFEIESHQECTYDQLIVYDGASIKNKQLGRFCGSKTPVPMKSQHNSILITFTS--DGSVQRRGFKITFNSICDGK 914
The following BLAST results are available for this feature:
BLAST of Metalloendopeptidase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Metalloendopeptidase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Metalloendopeptidase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Cubn3.525e-728.45gene:FBgn0052702 transcript:FBtr0273437[more]
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BLAST of Metalloendopeptidase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Metalloendopeptidase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
slain13.896e-626.49SLAIN motif family member 1 [Source:Xenbase;Acc:XB... [more]
wdr814.620e-625.55WD repeat domain 81 [Source:Xenbase;Acc:XB-GENE-62... [more]
mtx36.568e-627.21metaxin 3 [Source:Xenbase;Acc:XB-GENE-994127][more]
nol109.574e-627.56nucleolar protein 10 [Source:Xenbase;Acc:XB-GENE-5... [more]
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BLAST of Metalloendopeptidase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Metalloendopeptidase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|F1RWC3|CUBN_PIG1.434e-730.00Cubilin OS=Sus scrofa OX=9823 GN=CUBN PE=1 SV=3[more]
sp|Q9W332|CUBN_DROME3.534e-628.45Cubilin homolog OS=Drosophila melanogaster OX=7227... [more]
sp|Q9TU53|CUBN_CANLF7.499e-628.18Cubilin OS=Canis lupus familiaris OX=9615 GN=CUBN ... [more]
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BLAST of Metalloendopeptidase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8IF631.466e-1128.85CUB domain-containing protein OS=Macrostomum ligna... [more]
A0A2I9LPU73.867e-734.51Metalloendopeptidase OS=Centruroides hentzi OX=883... [more]
A0A482V1R99.880e-733.33Metalloendopeptidase (Fragment) OS=Asbolus verruco... [more]
A0A3M6V1S31.055e-627.14Uncharacterized protein OS=Pocillopora damicornis ... [more]
A0A139WL182.599e-633.33Metalloendopeptidase OS=Tribolium castaneum OX=707... [more]
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BLAST of Metalloendopeptidase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Metalloendopeptidase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Metalloendopeptidase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Metalloendopeptidase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO431876.387e-725.86Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO390034.893e-628.10Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO498237.036e-623.28Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Metalloendopeptidase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
cubn4.048e-629.36cubilin [Source:NCBI gene;Acc:105353827][more]
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BLAST of Metalloendopeptidase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000064589.13.700e-9398.59SMESG000064589.1[more]
SMESG000005882.12.765e-728.45SMESG000005882.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011992 ID=SMED30011992|Name=Metalloendopeptidase|organism=Schmidtea mediterranea sexual|type=transcript|length=691bp
TCTCAATTATTTGAAATGAAATTCTCTTTAACCGTTTTATTGATTTTGCA
CTGCCTCATTTTTGTCATTCAAGCCTCGTACGAAAAGAGCTGGAACAATG
CCTGCGATGATGAGAAAATGCGTCAGAACAATTGCGCATTTTACAAATTA
CTCAACGCATCGGAAAACGAAACGGAATTACGGTCTCCGAGCTACCCCAA
AACGAATACTTATGACATCATATGCCGGTGGTTGATTAATTCCACCTGCT
CTTCTACCAACTCATCCGTGACCCATGTCATCCAAATCAAATTGAACGAC
ATTGACATTTTAAATGCCCCATCATGTAGCAAAGGATATTTAGAAATAAG
AGACGGTGGCAGAGTTTGGGATTACTCCCAAGCAAAATTATGTGGAACAG
ATGGAGTTGATTCTTACATCAGTACAAACAGATATTTTCTTGTTACATTT
GTGAGTGGAAGAGATTCAATGAATCCTAAAAGAGGATTTTCGCTAACTTA
TAAATGCATTGCAAAAGATAAATACTTTGACCAGCCGGTAAATTTCAAAA
TCATAAATTACGTGCTGATATACATGAGCGTTGGAATTTTGTTCACTTTC
ACATTTCTGCTGACATTTATTATGTTAAAAGAATATCGTAAAACAATTAG
AATTTTTTGCATTCAAGAAAATTTAATTAATTCATGTCACA
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protein sequence of SMED30011992-orf-1

>SMED30011992-orf-1 ID=SMED30011992-orf-1|Name=SMED30011992-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=230bp
SQLFEMKFSLTVLLILHCLIFVIQASYEKSWNNACDDEKMRQNNCAFYKL
LNASENETELRSPSYPKTNTYDIICRWLINSTCSSTNSSVTHVIQIKLND
IDILNAPSCSKGYLEIRDGGRVWDYSQAKLCGTDGVDSYISTNRYFLVTF
VSGRDSMNPKRGFSLTYKCIAKDKYFDQPVNFKIINYVLIYMSVGILFTF
TFLLTFIMLKEYRKTIRIFCIQENLINSCH
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0004222metalloendopeptidase activity
GO:0008233peptidase activity
GO:0008237metallopeptidase activity
GO:0008270zinc ion binding
GO:0016787hydrolase activity
GO:0046872metal ion binding
GO:0005509calcium ion binding
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR035914Sperma_CUB_dom_sf
IPR000859CUB_dom
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000859CUB domainSMARTSM00042CUB_2coord: 45..170
e-value: 1.4E-5
score: 34.6
IPR000859CUB domainPFAMPF00431CUBcoord: 54..167
e-value: 6.7E-13
score: 48.9
IPR000859CUB domainPROSITEPS01180CUBcoord: 45..170
score: 15.313
IPR000859CUB domainCDDcd00041CUBcoord: 54..167
e-value: 1.01074E-17
score: 73.9858
IPR035914Spermadhesin, CUB domain superfamilyGENE3DG3DSA:2.60.120.290coord: 51..171
e-value: 9.8E-18
score: 66.6
IPR035914Spermadhesin, CUB domain superfamilySUPERFAMILYSSF49854Spermadhesin, CUB domaincoord: 57..169
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..25
score: 0.882
NoneNo IPR availableTMHMMTMhelixcoord: 187..209