sp6-9

Overview
Namesp6-9 (AFC87782.1)
Smed IDSMED30011934
Length (bp)865
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of sp6-9 (SMED30011934) t-SNE clustered cells

Violin plots show distribution of expression levels for sp6-9 (SMED30011934) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of sp6-9 (SMED30011934) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for sp6-9 (SMED30011934) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30011934

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 15

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30011934SMESG000050160.1 SmedASXL_006176SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
head regionSMED30011934SMESG000050160.1 dd_Smed_v6_17385_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
eyeSMED30011934SMESG000050160.1 sp6-9smed_ncbi_20200123PMID:25254346
Scimone et al., 2014
whole organism asexual adult colorimetric in situ hybridization evidence
central nervous systemSMED30011934SMESG000050160.1 sp6-9smed_ncbi_20200123PMID:25254346
Scimone et al., 2014
whole organism asexual adult colorimetric in situ hybridization evidence
eyeSMED30011934SMESG000050160.1 Contig37509newmark_estsPMID:27612384
Roberts-Galbraith et al., 2016
whole organism asexual adult colorimetric in situ hybridization evidence
eyeSMED30011934SMESG000050160.1 Contig37509uc_Smed_v2PMID:27612384
Roberts-Galbraith et al., 2016
whole organism asexual adult colorimetric in situ hybridization evidence
neuronSMED30011934SMESG000050160.1 Contig37509newmark_estsPMID:27612384
Roberts-Galbraith et al., 2016
whole organism asexual adult colorimetric in situ hybridization evidence
neuronSMED30011934SMESG000050160.1 Contig37509uc_Smed_v2PMID:27612384
Roberts-Galbraith et al., 2016
whole organism asexual adult colorimetric in situ hybridization evidence
pigment cup cellSMED30011934SMESG000050160.1 JN983830.1smed_ncbi_20200123PMID:28976975
Su et al., 2017
whole organism asexual adult fluorescence in situ hybridization evidence
trail cellSMED30011934SMESG000050160.1 JN983830.1smed_ncbi_20200123PMID:28976975
Su et al., 2017
whole organism asexual adult fluorescence in situ hybridization evidence
eyeSMED30011934SMESG000050160.1 sp6-9smed_ncbi_20200123PMID:21852957
Lapan et al., 2011
whole organism asexual adult fluorescence in situ hybridization evidence
cephalic gangliaSMED30011934SMESG000050160.1 sp6-9smed_ncbi_20200123PMID:21852957
Lapan et al., 2011
whole organism asexual adult fluorescence in situ hybridization evidence
trail cellSMED30011934SMESG000050160.1 sp6-9smed_ncbi_20200123PMID:21852957
Lapan et al., 2011
whole organism asexual adult fluorescence in situ hybridization evidence
ventral region of the whole animalSMED30011934SMESG000050160.1 sp6-9smed_ncbi_20200123PMID:21852957
Lapan et al., 2011
whole organism asexual adult fluorescence in situ hybridization evidence
head marginSMED30011934SMESG000050160.1 sp6-9smed_ncbi_20200123PMID:21852957
Lapan et al., 2011
whole organism asexual adult fluorescence in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
BLAST of sp6-9 vs. Ensembl Human
Match: SP9 (Sp9 transcription factor [Source:HGNC Symbol;Acc:HGNC:30690])

HSP 1 Score: 181.03 bits (458), Expect = 6.523e-53
Identity = 79/115 (68.70%), Postives = 88/115 (76.52%), Query Frame = 2
Query:  452 RYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKHI+THN
Sbjct:  301 RYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHIKTHN 415          
BLAST of sp6-9 vs. Ensembl Human
Match: SP8 (Sp8 transcription factor [Source:HGNC Symbol;Acc:HGNC:19196])

HSP 1 Score: 177.563 bits (449), Expect = 7.601e-52
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2
Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  297 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 414          
BLAST of sp6-9 vs. Ensembl Human
Match: SP8 (Sp8 transcription factor [Source:HGNC Symbol;Acc:HGNC:19196])

HSP 1 Score: 177.178 bits (448), Expect = 2.422e-51
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2
Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  339 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 456          
BLAST of sp6-9 vs. Ensembl Human
Match: SP8 (Sp8 transcription factor [Source:HGNC Symbol;Acc:HGNC:19196])

HSP 1 Score: 176.792 bits (447), Expect = 2.934e-51
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2
Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  321 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 438          
BLAST of sp6-9 vs. Ensembl Human
Match: SP4 (Sp4 transcription factor [Source:HGNC Symbol;Acc:HGNC:11209])

HSP 1 Score: 169.859 bits (429), Expect = 2.755e-47
Identity = 69/108 (63.89%), Postives = 82/108 (75.93%), Query Frame = 2
Query:  464 RAVCSCPNCQEMENL-SGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            R  CSCPNC+E E   S    K   H CHI GCGK+Y K SHL+AHLRWHTGERPF+CNW+FCGKRF RSDELQ+H + HT EK+  CP+C K F+RS+ LSKH++TH
Sbjct:  605 RVACSCPNCREGEGRGSNEPGKKKQHICHIEGCGKVYGKTSHLRAHLRWHTGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHVKTH 712          
BLAST of sp6-9 vs. Ensembl Celegans
Match: sptf-3 (SP (Specificity Protein) Transcription Factor [Source:UniProtKB/TrEMBL;Acc:Q9XW26])

HSP 1 Score: 120.168 bits (300), Expect = 3.866e-31
Identity = 55/107 (51.40%), Postives = 70/107 (65.42%), Query Frame = 2
Query:  464 RAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            R  C+CPNC +  N  G   K+  H CH+  C K Y K SHL+AHLR H G +PF C+W  C K+F RSDELQ+H + HT EK+  C  CGK F+RS+ L+KH +TH
Sbjct:  293 RCACTCPNCLQQNNRQGD-GKSRIHICHL--CNKTYGKTSHLRAHLRGHAGNKPFACDWQHCNKKFTRSDELQRHRRTHTGEKRFACNHCGKKFMRSDHLTKHERTH 396          
BLAST of sp6-9 vs. Ensembl Celegans
Match: sptf-2 (SP (Specificity Protein) Transcription Factor [Source:UniProtKB/TrEMBL;Acc:Q22678])

HSP 1 Score: 113.62 bits (283), Expect = 6.963e-31
Identity = 50/103 (48.54%), Postives = 71/103 (68.93%), Query Frame = 2
Query:  473 CSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQT 781
            C+CPNC+ +++  G     ++H C + GCGK Y K SHL+AHLR HTG+RPFVC+W  CGKRF+RSD+L +H + HT E +  C  C + F RS+ L +H+ +
Sbjct:   58 CTCPNCKAIKH--GDRGSQHTHLCSVPGCGKTYKKTSHLRAHLRKHTGDRPFVCDWFDCGKRFDRSDQLIRHKRTHTKEYRFACKFCIRQFSRSDHLQQHLTS 158          
BLAST of sp6-9 vs. Ensembl Celegans
Match: klf-3 (Kruppel-Like Factor (Zinc finger protein) [Source:UniProtKB/TrEMBL;Acc:P91329])

HSP 1 Score: 96.6709 bits (239), Expect = 4.249e-23
Identity = 43/84 (51.19%), Postives = 56/84 (66.67%), Query Frame = 2
Query:  536 HNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            H C   GC K YSK SHLKAH R H+GE+PFVC W  C  +F RSDEL +H++ HT +K   C  C ++F RS+ LS H++ H+
Sbjct:  224 HACTYPGCFKKYSKSSHLKAHERTHSGEKPFVCKWQNCSWKFARSDELTRHMRKHTGDKPFRCSLCDRNFARSDHLSLHMKRHS 307          
BLAST of sp6-9 vs. Ensembl Celegans
Match: klf-3 (Kruppel-Like Factor (Zinc finger protein) [Source:UniProtKB/TrEMBL;Acc:P91329])

HSP 1 Score: 96.6709 bits (239), Expect = 4.601e-23
Identity = 43/84 (51.19%), Postives = 56/84 (66.67%), Query Frame = 2
Query:  536 HNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            H C   GC K YSK SHLKAH R H+GE+PFVC W  C  +F RSDEL +H++ HT +K   C  C ++F RS+ LS H++ H+
Sbjct:  230 HACTYPGCFKKYSKSSHLKAHERTHSGEKPFVCKWQNCSWKFARSDELTRHMRKHTGDKPFRCSLCDRNFARSDHLSLHMKRHS 313          
BLAST of sp6-9 vs. Ensembl Celegans
Match: klf-2 (Kruppel-Like Factor (Zinc finger protein) [Source:UniProtKB/TrEMBL;Acc:O62259])

HSP 1 Score: 95.1301 bits (235), Expect = 1.432e-22
Identity = 42/94 (44.68%), Postives = 57/94 (60.64%), Query Frame = 2
Query:  518 LSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEK 799
            L +   H C   GCGK+Y+K SHL AH R H+GE+P+ C W  C  RF RSDEL +H + HT  K   C +C + F RS+ L  H++ H  +E+
Sbjct:  192 LDRLRVHKCFYQGCGKVYTKSSHLTAHERVHSGEKPYPCEWPGCSWRFARSDELTRHYRKHTGAKPFACKECSRKFSRSDHLQLHMKRHETDEQ 285          
BLAST of sp6-9 vs. Ensembl Fly
Match: Sp1 (gene:FBgn0020378 transcript:FBtr0071381)

HSP 1 Score: 182.185 bits (461), Expect = 1.905e-53
Identity = 80/118 (67.80%), Postives = 92/118 (77.97%), Query Frame = 2
Query:  443 SQPRYSNRAVCSCPNCQEMENLSG---YLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            SQ RY+ RA C CPNCQE E L     +L K N H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ L+KH++THN
Sbjct:  156 SQRRYAGRATCDCPNCQEAERLGPAGVHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLAKHVKTHN 273          
BLAST of sp6-9 vs. Ensembl Fly
Match: Sp1 (gene:FBgn0020378 transcript:FBtr0273354)

HSP 1 Score: 182.956 bits (463), Expect = 1.779e-52
Identity = 80/118 (67.80%), Postives = 92/118 (77.97%), Query Frame = 2
Query:  443 SQPRYSNRAVCSCPNCQEMENLSG---YLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            SQ RY+ RA C CPNCQE E L     +L K N H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ L+KH++THN
Sbjct:  351 SQRRYAGRATCDCPNCQEAERLGPAGVHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLAKHVKTHN 468          
BLAST of sp6-9 vs. Ensembl Fly
Match: Sp1 (gene:FBgn0020378 transcript:FBtr0310290)

HSP 1 Score: 182.956 bits (463), Expect = 1.795e-52
Identity = 80/118 (67.80%), Postives = 92/118 (77.97%), Query Frame = 2
Query:  443 SQPRYSNRAVCSCPNCQEMENLSG---YLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            SQ RY+ RA C CPNCQE E L     +L K N H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ L+KH++THN
Sbjct:  356 SQRRYAGRATCDCPNCQEAERLGPAGVHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLAKHVKTHN 473          
BLAST of sp6-9 vs. Ensembl Fly
Match: Sp1 (gene:FBgn0020378 transcript:FBtr0331335)

HSP 1 Score: 182.956 bits (463), Expect = 2.064e-52
Identity = 80/118 (67.80%), Postives = 92/118 (77.97%), Query Frame = 2
Query:  443 SQPRYSNRAVCSCPNCQEMENLSG---YLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            SQ RY+ RA C CPNCQE E L     +L K N H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ L+KH++THN
Sbjct:  351 SQRRYAGRATCDCPNCQEAERLGPAGVHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLAKHVKTHN 468          
BLAST of sp6-9 vs. Ensembl Fly
Match: Spps (gene:FBgn0039169 transcript:FBtr0084565)

HSP 1 Score: 150.599 bits (379), Expect = 1.323e-40
Identity = 69/115 (60.00%), Postives = 82/115 (71.30%), Query Frame = 2
Query:  446 QPRYSNRAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNK 790
            +PR   R  C+CPNC + E  S    K   H CHI GC K+Y K SHL+AHLRWHTGERPFVC+W FCGKRF RSDELQ+H + HT EK+  C +C K F+RS+ LSKHI+TH K
Sbjct:  714 KPRL-KRVACTCPNCTDGEKHS---DKKRQHICHITGCHKVYGKTSHLRAHLRWHTGERPFVCSWAFCGKRFTRSDELQRHRRTHTGEKRFQCQECNKKFMRSDHLSKHIKTHFK 824          
BLAST of sp6-9 vs. Ensembl Zebrafish
Match: sp8a (sp8 transcription factor a [Source:ZFIN;Acc:ZDB-GENE-030131-9849])

HSP 1 Score: 180.644 bits (457), Expect = 1.160e-53
Identity = 79/122 (64.75%), Postives = 92/122 (75.41%), Query Frame = 2
Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEE 796
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+ +CP C K F+RS+ LSKH++TH+  E
Sbjct:  245 SSTRRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFSCPVCNKRFMRSDHLSKHVKTHSAGE 366          
BLAST of sp6-9 vs. Ensembl Zebrafish
Match: sp8a (sp8 transcription factor a [Source:ZFIN;Acc:ZDB-GENE-030131-9849])

HSP 1 Score: 179.874 bits (455), Expect = 1.190e-53
Identity = 79/122 (64.75%), Postives = 92/122 (75.41%), Query Frame = 2
Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEE 796
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+ +CP C K F+RS+ LSKH++TH+  E
Sbjct:  227 SSTRRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFSCPVCNKRFMRSDHLSKHVKTHSAGE 348          
BLAST of sp6-9 vs. Ensembl Zebrafish
Match: sp8b (sp8 transcription factor b [Source:NCBI gene;Acc:324931])

HSP 1 Score: 178.333 bits (451), Expect = 1.347e-52
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2
Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  262 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 379          
BLAST of sp6-9 vs. Ensembl Zebrafish
Match: sp8b (sp8 transcription factor b [Source:NCBI gene;Acc:324931])

HSP 1 Score: 178.333 bits (451), Expect = 1.347e-52
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2
Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  262 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 379          
BLAST of sp6-9 vs. Ensembl Zebrafish
Match: sp8b (sp8 transcription factor b [Source:NCBI gene;Acc:324931])

HSP 1 Score: 178.718 bits (452), Expect = 1.448e-52
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2
Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  280 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 397          
BLAST of sp6-9 vs. Ensembl Xenopus
Match: sp9 (Sp9 transcription factor [Source:NCBI gene;Acc:779722])

HSP 1 Score: 181.415 bits (459), Expect = 9.848e-54
Identity = 80/118 (67.80%), Postives = 89/118 (75.42%), Query Frame = 2
Query:  443 SQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKHI+THN
Sbjct:  261 SARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHIKTHN 378          
BLAST of sp6-9 vs. Ensembl Xenopus
Match: sp8 (Xenopus tropicalis Sp8 transcription factor (sp8), mRNA. [Source:RefSeq mRNA;Acc:NM_001292062])

HSP 1 Score: 175.637 bits (444), Expect = 1.122e-50
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2
Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  350 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 467          
BLAST of sp6-9 vs. Ensembl Xenopus
Match: bsdc1 (BSD domain containing 1 [Source:Xenbase;Acc:XB-GENE-5892016])

HSP 1 Score: 167.162 bits (422), Expect = 3.632e-48
Identity = 73/119 (61.34%), Postives = 87/119 (73.11%), Query Frame = 2
Query:  458 SNRAVCSCPNCQEMENL---SGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSK 805
            S R  C CPNCQE+E L   +  L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDEL++H++ HT EKK  CP C K F RS+ LSKH ++H  +  +K
Sbjct:  262 SGRPSCDCPNCQELERLGASAASLKRKPVHSCHIPGCGKVYGKASHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELERHVRTHTREKKFTCPLCAKRFTRSDHLSKHQRSHVDQPGAK 380          
BLAST of sp6-9 vs. Ensembl Xenopus
Match: smox (spermine oxidase [Source:Xenbase;Acc:XB-GENE-5840847])

HSP 1 Score: 162.925 bits (411), Expect = 2.825e-47
Identity = 72/122 (59.02%), Postives = 88/122 (72.13%), Query Frame = 2
Query:  443 SQPRYSNRAVCSCPNCQEMENLSGYLSK---NNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEK 799
            + PR + +  C CPNCQE E + G        N HNCHI GCGK Y+K SHLKAHLRWH+G+RPFVCNWLFCGKRF RSDELQ+HL+ HT  KK  CP C + F+RS+ L+KH++TH  E +
Sbjct:  195 AMPRSAAQTACRCPNCQEAERVGGGGEGGKHKNLHNCHIPGCGKAYAKTSHLKAHLRWHSGDRPFVCNWLFCGKRFTRSDELQRHLQTHTGAKKYPCPSCSRVFMRSDHLTKHMKTHEGESR 316          
BLAST of sp6-9 vs. Ensembl Xenopus
Match: zp3.2 (zona pellucida glycoprotein 3 (sperm receptor), gene 2 [Source:Xenbase;Acc:XB-GENE-1019608])

HSP 1 Score: 166.777 bits (421), Expect = 1.522e-46
Identity = 67/115 (58.26%), Postives = 83/115 (72.17%), Query Frame = 2
Query:  440 NSQPRYSNRAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            N + +   R  C+CPNC++       L K   H CHI GCGK+Y K SHL+AHLRWH+GERPFVC W+FCGKRF RSDELQ+H + HT EKK  CP+C K F+RS+ L+KHI+TH
Sbjct:  473 NQEGKRLRRVACTCPNCKDGAGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCTWMFCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTH 587          
BLAST of sp6-9 vs. Ensembl Mouse
Match: Sp9 (trans-acting transcription factor 9 [Source:MGI Symbol;Acc:MGI:3574660])

HSP 1 Score: 181.03 bits (458), Expect = 4.117e-53
Identity = 79/115 (68.70%), Postives = 88/115 (76.52%), Query Frame = 2
Query:  452 RYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKHI+THN
Sbjct:  301 RYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHIKTHN 415          
BLAST of sp6-9 vs. Ensembl Mouse
Match: Sp8 (trans-acting transcription factor 8 [Source:MGI Symbol;Acc:MGI:2443471])

HSP 1 Score: 176.792 bits (447), Expect = 1.513e-51
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2
Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  321 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 438          
BLAST of sp6-9 vs. Ensembl Mouse
Match: Sp4 (trans-acting transcription factor 4 [Source:MGI Symbol;Acc:MGI:107595])

HSP 1 Score: 171.4 bits (433), Expect = 5.846e-48
Identity = 69/107 (64.49%), Postives = 82/107 (76.64%), Query Frame = 2
Query:  464 RAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            R  CSCPNC+E E  S    K   H CHI GCGK+Y K SHL+AHLRWHTGERPF+CNW+FCGKRF RSDELQ+H + HT EK+  CP+C K F+RS+ LSKH++TH
Sbjct:  620 RVACSCPNCREGEG-SSEPGKKKQHVCHIEGCGKVYGKTSHLRAHLRWHTGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHVKTH 725          
BLAST of sp6-9 vs. Ensembl Mouse
Match: Sp4 (trans-acting transcription factor 4 [Source:MGI Symbol;Acc:MGI:107595])

HSP 1 Score: 170.629 bits (431), Expect = 1.295e-47
Identity = 69/108 (63.89%), Postives = 82/108 (75.93%), Query Frame = 2
Query:  464 RAVCSCPNCQEMENL-SGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            R  CSCPNC+E E   S    K   H CHI GCGK+Y K SHL+AHLRWHTGERPF+CNW+FCGKRF RSDELQ+H + HT EK+  CP+C K F+RS+ LSKH++TH
Sbjct:  620 RVACSCPNCREGEGRGSSEPGKKKQHVCHIEGCGKVYGKTSHLRAHLRWHTGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHVKTH 727          
BLAST of sp6-9 vs. Ensembl Mouse
Match: Sp3 (trans-acting transcription factor 3 [Source:MGI Symbol;Acc:MGI:1277166])

HSP 1 Score: 169.474 bits (428), Expect = 2.517e-47
Identity = 67/107 (62.62%), Postives = 81/107 (75.70%), Query Frame = 2
Query:  464 RAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            R  C+CPNC+E       L K   H CHI GCGK+Y K SHL+AHLRWH+GERPF+CNW+FCGKRF RSDELQ+H + HT EKK  CP+C K F+RS+ L+KHI+TH
Sbjct:  555 RVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTH 661          
BLAST of sp6-9 vs. UniProt/SwissProt
Match: sp|Q0VA40|SP9_XENTR (Transcription factor Sp9 OS=Xenopus tropicalis OX=8364 GN=sp9 PE=2 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 5.217e-53
Identity = 80/118 (67.80%), Postives = 89/118 (75.42%), Query Frame = 2
Query:  443 SQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKHI+THN
Sbjct:  261 SARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHIKTHN 378          
BLAST of sp6-9 vs. UniProt/SwissProt
Match: sp|Q64HY3|SP9_MOUSE (Transcription factor Sp9 OS=Mus musculus OX=10090 GN=Sp9 PE=1 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 2.880e-52
Identity = 79/115 (68.70%), Postives = 88/115 (76.52%), Query Frame = 2
Query:  452 RYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKHI+THN
Sbjct:  301 RYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHIKTHN 415          
BLAST of sp6-9 vs. UniProt/SwissProt
Match: sp|P0CG40|SP9_HUMAN (Transcription factor Sp9 OS=Homo sapiens OX=9606 GN=SP9 PE=3 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 3.133e-52
Identity = 79/115 (68.70%), Postives = 88/115 (76.52%), Query Frame = 2
Query:  452 RYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKHI+THN
Sbjct:  301 RYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHIKTHN 415          
BLAST of sp6-9 vs. UniProt/SwissProt
Match: sp|Q6P0J3|SP8_DANRE (Transcription factor Sp8 OS=Danio rerio OX=7955 GN=sp8b PE=2 SV=1)

HSP 1 Score: 178.333 bits (451), Expect = 1.017e-51
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2
Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  262 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 379          
BLAST of sp6-9 vs. UniProt/SwissProt
Match: sp|Q64HY5|SP8_CHICK (Transcription factor Sp8 OS=Gallus gallus OX=9031 GN=SP8 PE=2 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 5.019e-51
Identity = 78/119 (65.55%), Postives = 90/119 (75.63%), Query Frame = 2
Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH+
Sbjct:  307 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTHS 425          
BLAST of sp6-9 vs. TrEMBL
Match: H9B8J7 (Sp6-9 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 473.011 bits (1216), Expect = 1.188e-167
Identity = 227/228 (99.56%), Postives = 227/228 (99.56%), Query Frame = 2
Query:  134 MNNTAIQNIWRFTFPYHDQKIHQYPSTQSDVNNGFHPFLSLPNYQSLTSSNPYLPSVQNELALNLSDSHILNLPPTPNFDKHTFQSIPANYFNPFISTFYSGNSQPRYSNRAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSKLTSR 817
            MNNTAIQNIWRFTFPYHDQKIHQYPSTQSDVNNGFHPFLS PNYQSLTSSNPYLPSVQNELALNLSDSHILNLPPTPNFDKHTFQSIPANYFNPFISTFYSGNSQPRYSNRAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSKLTSR
Sbjct:    1 MNNTAIQNIWRFTFPYHDQKIHQYPSTQSDVNNGFHPFLSFPNYQSLTSSNPYLPSVQNELALNLSDSHILNLPPTPNFDKHTFQSIPANYFNPFISTFYSGNSQPRYSNRAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSKLTSR 228          
BLAST of sp6-9 vs. TrEMBL
Match: A0A515MD64 (Sp6-9 (Fragment) OS=Aphonopelma hentzi OX=231250 GN=Sp6-9 PE=2 SV=1)

HSP 1 Score: 194.897 bits (494), Expect = 1.589e-55
Identity = 112/259 (43.24%), Postives = 140/259 (54.05%), Query Frame = 2
Query:   50 NVESNYQSWLLERYKSVSKINPITNENEMNNTAIQNIW----RFTFPYHDQKIHQYPSTQSDVNNGFHPFLSLPNYQSLTSSNPYLPSVQNELALNLSDSHILNLPPTPNFDKHTFQSIPANYFNPFISTFYSGNSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSK 805
            NV     + +     SV+ +N  T+  +M+  +    W      T   H Q    YPS+    + G  P L+  N   L SS  +L          L D++   LP           + P +   P +    S  SQ RYS RA C CPNCQE E L     +L K N H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++THN    +K
Sbjct:  152 NVAGATHTGIKTEVSSVNTLN-STSWWDMHTASSAGSWLDMSGATTGLHSQLPANYPSS----DYGLGPSLAASNTAHLLSSGQHL----------LQDTYKSMLPSAQGVGPSV--ASPFSLSQPTLPQMPSPRSQRRYSGRATCDCPNCQEAERLGPAGAHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPICNKRFMRSDHLSKHVKTHNDNGGTK 393          
BLAST of sp6-9 vs. TrEMBL
Match: W4YK02 (Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 2.622e-55
Identity = 91/177 (51.41%), Postives = 115/177 (64.97%), Query Frame = 2
Query:  263 YQSLTSSNPYLPSVQNELALNLSDSHILNLPPTPNFDKHTFQSIPANYFNPFISTFYSGNSQPRYSNRAVCSCPNCQEMENLSGY---LSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            Y  LT++NP+L S       +  +S +      P +D  +   + +++F P         +  RYS RA C CPNCQE E L+     L K N H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+ +CP C K F+RS+ LSKH++TH
Sbjct:   22 YPPLTTTNPHLFSPSQLFPPSSYNSFL-----PPGYDIMSLTEMRSSFFGPAGLGLSLSRAGRRYSGRATCDCPNCQENERLAAAGQPLRKKNVHSCHIPGCGKIYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFSCPTCNKRFMRSDHLSKHVKTH 193          
BLAST of sp6-9 vs. TrEMBL
Match: A0A2R4FYF3 (Zinc finger transcription factor Sp6/9 OS=Centruroides sculpturatus OX=218467 GN=Sp6 PE=2 SV=1)

HSP 1 Score: 192.971 bits (489), Expect = 1.510e-54
Identity = 112/256 (43.75%), Postives = 141/256 (55.08%), Query Frame = 2
Query:   50 NVESNYQSWLLERYKSVSKINPITNENEMNNTAIQNIW----RFTFPYHDQKIHQYPSTQSDVNNGFHPFLSLPNYQSLTSSNPYLPSVQNELALNLSDSHILNLPPTPNFDKHTFQSIPANYFN---PFISTFYSGNSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            NV     S +     +V+ +N  T+  +M+  +    W      T   H Q    YPS++     G  P L+  N   L SS  +L          L D++   LP        + Q + A+ F    P +    S  SQ RY+ RA C CPNCQE E L     +L K N H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++THN
Sbjct:  170 NVAGAAHSGIKPEVSTVNSLN-STSWWDMHAASGAGSWLDMSGATSGLHSQLPTNYPSSE----YGLAPTLAASNTAHLLSSGQHL----------LQDTYKSMLP--------SAQGVGASPFGLSQPALPQMPSPRSQRRYTGRATCDCPNCQEAERLGPAGAHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPICNKRFMRSDHLSKHVKTHN 402          
BLAST of sp6-9 vs. TrEMBL
Match: A0A2H4PSJ4 (Zinc finger transcription factor Sp6-9 OS=Parasteatoda tepidariorum OX=114398 PE=2 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 4.281e-54
Identity = 84/122 (68.85%), Postives = 95/122 (77.87%), Query Frame = 2
Query:  443 SQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEK 799
            SQ RYS RA C CPNCQE E L     +L K N H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP CGK F+RS+ L+KH++THN  EK
Sbjct:  272 SQRRYSGRATCDCPNCQEAERLGPAGAHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFVCPNCGKRFMRSDHLAKHVKTHNGSEK 393          
BLAST of sp6-9 vs. Ensembl Cavefish
Match: sp8a (Sp8 transcription factor [Source:NCBI gene;Acc:103039368])

HSP 1 Score: 181.03 bits (458), Expect = 1.754e-53
Identity = 79/118 (66.95%), Postives = 90/118 (76.27%), Query Frame = 2
Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP CGK F+RS+ LSKH++TH
Sbjct:  291 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCGKRFMRSDHLSKHVKTH 408          
BLAST of sp6-9 vs. Ensembl Cavefish
Match: ENSAMXT00000020600.2 (pep primary_assembly:Astyanax_mexicanus-2.0:10:32985937:32991833:-1 gene:ENSAMXG00000020011.2 transcript:ENSAMXT00000020600.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 178.718 bits (452), Expect = 9.850e-53
Identity = 75/115 (65.22%), Postives = 89/115 (77.39%), Query Frame = 2
Query:  455 YSNRAVCSCPNCQEMENL----SGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            Y+ RA C CPNCQE E L    +  + +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP CGK F+RS+ LSKH++TH+
Sbjct:  287 YAGRATCDCPNCQEAERLGPAGAASMRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCGKRFMRSDHLSKHVKTHS 401          
BLAST of sp6-9 vs. Ensembl Cavefish
Match: sp4 (Sp4 transcription factor [Source:NCBI gene;Acc:103039672])

HSP 1 Score: 174.096 bits (440), Expect = 3.896e-49
Identity = 73/116 (62.93%), Postives = 89/116 (76.72%), Query Frame = 2
Query:  464 RAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH-NKEEKSKL 808
            R  CSCPNC++ E  +   SK   H CH+AGCGK+Y K SHL+AHLRWHTGERPFVCNW+FCGKRF RSDELQ+H + HT EK+  CP+C K F+RS+ LSKHI+TH NK+  + L
Sbjct:  594 RVACSCPNCRDGEGRNSDPSKKKQHVCHMAGCGKVYGKTSHLRAHLRWHTGERPFVCNWIFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHIKTHQNKKGGTAL 709          
BLAST of sp6-9 vs. Ensembl Cavefish
Match: sp9 (sp9 transcription factor [Source:NCBI gene;Acc:405896])

HSP 1 Score: 168.703 bits (426), Expect = 6.216e-49
Identity = 73/109 (66.97%), Postives = 82/109 (75.23%), Query Frame = 2
Query:  470 VCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
             C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKHI+TH 
Sbjct:  285 TCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHIKTHT 393          
BLAST of sp6-9 vs. Ensembl Cavefish
Match: sp3a (Sp3 transcription factor [Source:NCBI gene;Acc:103031127])

HSP 1 Score: 171.785 bits (434), Expect = 1.467e-48
Identity = 68/116 (58.62%), Postives = 84/116 (72.41%), Query Frame = 2
Query:  464 RAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSKLT 811
            R  C+CPNC+E       + K   H CHI GCGK+Y K SHL+AHLRWH+GERPFVCNW+FCGKRF RSDELQ+H + HT EKK  CP+C K F+RS+ L+KHI+TH  ++    T
Sbjct:  489 RVACTCPNCKEGGGRGTSIGKKKQHVCHIVGCGKVYGKTSHLRAHLRWHSGERPFVCNWMFCGKRFTRSDELQRHRRTHTGEKKFVCPQCSKRFMRSDHLAKHIKTHQNKKGGGGT 604          
BLAST of sp6-9 vs. Ensembl Sea Lamprey
Match: sp4 (sp4 transcription factor [Source:ZFIN;Acc:ZDB-GENE-031202-3])

HSP 1 Score: 159.844 bits (403), Expect = 1.037e-46
Identity = 70/111 (63.06%), Postives = 80/111 (72.07%), Query Frame = 2
Query:  461 NRAVCSCPNCQEMENL-SGYL-SKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
             R  C+CPNC++ E   SG    K   H CHI GC K+Y K SHL+AHLRWHTGERPFVC WLFCGKRF RSDELQ+H + HT EKK  CP C K F+RS+ LSKHI+TH 
Sbjct:  267 RRVACACPNCRDGEGRRSGSEPGKKKQHVCHIPGCSKVYGKTSHLRAHLRWHTGERPFVCGWLFCGKRFTRSDELQRHRRTHTGEKKFPCPHCSKRFMRSDHLSKHIRTHQ 377          
BLAST of sp6-9 vs. Ensembl Sea Lamprey
Match: sp4 (sp4 transcription factor [Source:ZFIN;Acc:ZDB-GENE-031202-3])

HSP 1 Score: 160.614 bits (405), Expect = 2.288e-46
Identity = 73/119 (61.34%), Postives = 85/119 (71.43%), Query Frame = 2
Query:  464 RAVCSCPNCQEMENL-SGYL-SKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSKLTS 814
            R  C+CPNC++ E   SG    K   H CHI GC K+Y K SHL+AHLRWHTGERPFVC WLFCGKRF RSDELQ+H + HT EKK  CP C K F+RS+ LSKHI+TH + +KS L  
Sbjct:  312 RVACACPNCRDGEGRRSGSEPGKKKQHVCHIPGCSKVYGKTSHLRAHLRWHTGERPFVCGWLFCGKRFTRSDELQRHRRTHTGEKKFPCPHCSKRFMRSDHLSKHIRTH-QNKKSGLVG 429          
BLAST of sp6-9 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008916.1 (pep scaffold:Pmarinus_7.0:GL476670:39536:47416:-1 gene:ENSPMAG00000008063.1 transcript:ENSPMAT00000008916.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 155.221 bits (391), Expect = 2.953e-43
Identity = 64/111 (57.66%), Postives = 81/111 (72.97%), Query Frame = 2
Query:  464 RAVCSCPNCQEMEN-LSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKE 793
            R  C CPNC++ E  ++G   +   H CHI GC K+Y K SHL+AHLRWH+GERPFVC WL CGKRF RSDELQ+H + HT EKK  C +C K F+RS+ LSKH++TH+ +
Sbjct:  561 RVPCMCPNCRDSEGKVTGEAGQKKQHICHITGCSKVYGKTSHLRAHLRWHSGERPFVCTWLHCGKRFTRSDELQRHKRTHTGEKKFICSECSKRFMRSDHLSKHMKTHSAK 671          
BLAST of sp6-9 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000432.1 (pep scaffold:Pmarinus_7.0:GL477956:42837:43256:-1 gene:ENSPMAG00000000389.1 transcript:ENSPMAT00000000432.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 134.806 bits (338), Expect = 3.744e-40
Identity = 56/107 (52.34%), Postives = 71/107 (66.36%), Query Frame = 2
Query:  473 CSCPNCQEMENLSGYLSKNN-SHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNK 790
            C CPNCQ   N +   S +   H CH++GCGK Y K SHL+AHLRWH GERP+ C W  C +RF RSDEL++H + HT E++  C  CGK F RS+ L KH +TH +
Sbjct:   16 CRCPNCQTASNTATISSSSRRQHTCHVSGCGKAYGKTSHLRAHLRWHAGERPYACAWPLCARRFTRSDELRRHARTHTGERRFACVACGKRFARSDHLGKHARTHRE 122          
BLAST of sp6-9 vs. Ensembl Sea Lamprey
Match: sp8b (sp8 transcription factor b [Source:NCBI gene;Acc:324931])

HSP 1 Score: 135.576 bits (340), Expect = 1.632e-38
Identity = 63/97 (64.95%), Postives = 67/97 (69.07%), Query Frame = 2
Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQ---KHLKVHTDEK 712
             S  R+  RA C CPNCQE E L      L +   HNCHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ   KH K H+  K
Sbjct:  189 GSSRRFPGRATCDCPNCQEAERLGPAGASLRRKGLHNCHIPGCGKVYGKSSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQHLNKHAKTHSGGK 285          
BLAST of sp6-9 vs. Ensembl Yeast
Match: AZF1 (Zinc-finger transcription factor; involved in diauxic shift; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity; relocalizes to the cytosol in response to hypoxia [Source:SGD;Acc:S000005639])

HSP 1 Score: 78.5666 bits (192), Expect = 8.167e-17
Identity = 34/78 (43.59%), Postives = 50/78 (64.10%), Query Frame = 2
Query:  557 CGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNK 790
            C +L+S+ +HL+ H+R H G +PFVC++  CGKRF +   L+ H ++HT EK  +C  C K F R   L+ H+ TH K
Sbjct:  598 CHRLFSQATHLEVHVRSHIGYKPFVCDY--CGKRFTQGGNLRTHERLHTGEKPYSCDICDKKFSRKGNLAAHLVTHQK 673          

HSP 2 Score: 56.9954 bits (136), Expect = 1.071e-9
Identity = 30/89 (33.71%), Postives = 46/89 (51.69%), Query Frame = 2
Query:  557 CGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNC--PKCGKHFVRSEQLSKHIQTHNKEEKSKLTSR 817
            CGK +++  +L+ H R HTGE+P+ C+   C K+F+R   L  HL  H   K   C    C K F +   +  H    +KE  + LT++
Sbjct:  626 CGKRFTQGGNLRTHERLHTGEKPYSCD--ICDKKFSRKGNLAAHLVTHQKLKPFVCKLENCNKTFTQLGNMKAHQNRFHKETLNALTAK 712          
BLAST of sp6-9 vs. Ensembl Yeast
Match: CRZ1 (Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress; can be activated in stochastic pulses of nuclear localization in response to calcium [Source:SGD;Acc:S000004972])

HSP 1 Score: 75.8702 bits (185), Expect = 5.714e-16
Identity = 35/85 (41.18%), Postives = 51/85 (60.00%), Query Frame = 2
Query:  557 CGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCP---------KCGKHFVRSEQLSKHIQTH 784
            CGK +++P +LK+HLR HT ERPF+C+   CGK F R  + ++H  +HT +K+  C           CGK F RS+ L +H +T 
Sbjct:  574 CGKKFTRPYNLKSHLRTHTNERPFICS--ICGKAFARQHDRKRHEDLHTGKKRYVCGGKLKDGKPWGCGKKFARSDALGRHFKTE 656          

HSP 2 Score: 46.9802 bits (110), Expect = 2.186e-6
Identity = 21/58 (36.21%), Postives = 31/58 (53.45%), Query Frame = 2
Query:  626 FVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEK 799
            F C+   CGK+F R   L+ HL+ HT+E+   C  CGK F R     +H   H  +++
Sbjct:  569 FACD--VCGKKFTRPYNLKSHLRTHTNERPFICSICGKAFARQHDRKRHEDLHTGKKR 624          
BLAST of sp6-9 vs. Ensembl Yeast
Match: FZF1 (Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000003223])

HSP 1 Score: 72.4034 bits (176), Expect = 3.748e-15
Identity = 39/99 (39.39%), Postives = 59/99 (59.60%), Query Frame = 2
Query:  521 SKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSKLTSR 817
            +K+ ++ C   GC K+Y++PS L+ H   HT ++P+ C+   CGK+F R   L+ H   H+  K   C  C K FV ++QL +H+ +H  E KSKL SR
Sbjct:    7 TKSRNYKCSFDGCEKVYNRPSLLQQHQNSHTNQKPYHCDEPGCGKKFIRPCHLRVHKWTHSQIKPKACTLCQKRFVTNQQLRRHLNSH--ERKSKLASR 103          
BLAST of sp6-9 vs. Ensembl Yeast
Match: ZAP1 (Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains [Source:SGD;Acc:S000003592])

HSP 1 Score: 71.2478 bits (173), Expect = 2.629e-14
Identity = 37/87 (42.53%), Postives = 50/87 (57.47%), Query Frame = 2
Query:  557 CGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSKLTSR 817
            C + +S    L  H R H+GE+P+ C+   C K+F  S  L+ H++ HT EK L C  CGK F  S  LSKHI+TH K+ K    S+
Sbjct:  773 CKRCFSSEETLVQHTRTHSGEKPYKCH--ICNKKFAISSSLKIHIRTHTGEKPLQCKICGKRFNESSNLSKHIKTHQKKYKCSDCSK 857          

HSP 2 Score: 63.1586 bits (152), Expect = 9.684e-12
Identity = 34/68 (50.00%), Postives = 40/68 (58.82%), Query Frame = 2
Query:  542 CHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHF 745
            CHI  C K ++  S LK H+R HTGE+P  C    CGKRFN S  L KH+K H  +KK  C  C K F
Sbjct:  798 CHI--CNKKFAISSSLKIHIRTHTGEKPLQCK--ICGKRFNESSNLSKHIKTH--QKKYKCSDCSKSF 859          

HSP 3 Score: 51.6026 bits (122), Expect = 7.564e-8
Identity = 29/88 (32.95%), Postives = 42/88 (47.73%), Query Frame = 2
Query:  542 CHIAGCGKLYSKPSHLKAHLR---WHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEE 796
            C   GC K +S    L  HL       G+  + C W  C + F +  +L +HLKVH+  K   C  C + F   E L +H +TH+ E+
Sbjct:  707 CQWDGCNKSFSSAQELNDHLEAVHLTRGKSEYQCLWHDCHRTFPQRQKLIRHLKVHSKYKPYKCKTCKRCFSSEETLVQHTRTHSGEK 794          
BLAST of sp6-9 vs. Ensembl Yeast
Match: MIG2 (Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced gene repression; under low glucose conditions relocalizes to mitochondrion, where it interacts with Ups1p, antagonizes mitochondrial fission factor Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth in response to glucose depletion; activated in stochastic pulses of nuclear localization in response to low glucose [Source:SGD;Acc:S000003177])

HSP 1 Score: 60.077 bits (144), Expect = 8.048e-11
Identity = 25/59 (42.37%), Postives = 37/59 (62.71%), Query Frame = 2
Query:  557 CGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKC 733
            C + + +  H K HLR HTGE+P  C +  CGK F+RSDEL++H++ HT + +    K 
Sbjct:   22 CHRGFHRLEHKKRHLRTHTGEKPHHCAFPGCGKSFSRSDELKRHMRTHTGQSQRRLKKA 80          

HSP 2 Score: 55.4546 bits (132), Expect = 2.538e-9
Identity = 26/65 (40.00%), Postives = 42/65 (64.62%), Query Frame = 2
Query:  620 RPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNC--PKCGKHFVRSEQLSKHIQTHNKEEKSKL 808
            RPF C+   C + F+R +  ++HL+ HT EK  +C  P CGK F RS++L +H++TH  + + +L
Sbjct:   15 RPFRCDT--CHRGFHRLEHKKRHLRTHTGEKPHHCAFPGCGKSFSRSDELKRHMRTHTGQSQRRL 77          
BLAST of sp6-9 vs. Ensembl Nematostella
Match: EDO42885 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S016])

HSP 1 Score: 177.178 bits (448), Expect = 2.960e-53
Identity = 83/145 (57.24%), Postives = 99/145 (68.28%), Query Frame = 2
Query:  380 TFQSIPANYFNPFISTFYSGN------SQPRYSNRAVCSCPNCQEMENLSGY---LSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            T+   PA+YF P  + F          +  RY+ RA C CPNCQ+ E +S       K + H CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++THN
Sbjct:  210 TYIDGPASYF-PGTAAFLQSTPSLVPRTTRRYTGRATCDCPNCQDNERMSASGAPFRKKSQHICHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPICSKRFMRSDHLSKHVKTHN 353          
BLAST of sp6-9 vs. Ensembl Nematostella
Match: EDO42941 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S019])

HSP 1 Score: 152.91 bits (385), Expect = 5.666e-47
Identity = 67/112 (59.82%), Postives = 84/112 (75.00%), Query Frame = 2
Query:  452 RYSNRAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            R   R  C+CPNC++ E  +    K   H CH+ GCGK+Y K SHL+AHLRWH+GERPFVCNWLFCGKRF RSDELQ+H + HT EK+  CP+C K F+RS+ LSKH++TH+
Sbjct:    5 RRLRRIACTCPNCRDGEGRTANGRKQ--HVCHVPGCGKVYGKTSHLRAHLRWHSGERPFVCNWLFCGKRFTRSDELQRHRRTHTGEKRFTCPECNKKFMRSDHLSKHVKTHS 114          
BLAST of sp6-9 vs. Ensembl Nematostella
Match: EDO42939 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S017])

HSP 1 Score: 140.198 bits (352), Expect = 2.252e-42
Identity = 62/88 (70.45%), Postives = 70/88 (79.55%), Query Frame = 2
Query:  521 SKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            SK   H CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGK F RSDELQ+HL+ HT EK+  C +CGK F+RS+ L KH +TH
Sbjct:    3 SKRKQHVCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKSFTRSDELQRHLRTHTGEKRFQCDECGKRFMRSDHLRKHQKTH 90          

HSP 2 Score: 46.2098 bits (108), Expect = 6.854e-7
Identity = 23/59 (38.98%), Postives = 37/59 (62.71%), Query Frame = 2
Query:  629 VCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPK--CGKHFVRSEQLSKHIQTHNKEEK 799
            VC+   CGK + ++  L+ HL+ HT E+   C    CGK F RS++L +H++TH  E++
Sbjct:    9 VCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKSFTRSDELQRHLRTHTGEKR 67          
BLAST of sp6-9 vs. Ensembl Nematostella
Match: EDO42119 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2C5])

HSP 1 Score: 128.257 bits (321), Expect = 3.556e-37
Identity = 62/115 (53.91%), Postives = 75/115 (65.22%), Query Frame = 2
Query:  464 RAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWL-----FCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKE 793
            + +C+CPNC    N +   +    HNCH  GCGK+Y K SHLKAHLRWH GERPFVCNW       CGK F RSDELQ+HL+ HT EK   C  CGK F RS+ L+KH++ H  +
Sbjct:    1 KCLCACPNCVSGINSNPSPTHPKYHNCHYPGCGKIYGKTSHLKAHLRWHRGERPFVCNWQTILGSTCGKSFTRSDELQRHLRTHTKEKAHTCHVCGKAFGRSDHLTKHLRIHKAK 115          
BLAST of sp6-9 vs. Ensembl Nematostella
Match: EDO32290 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SVG8])

HSP 1 Score: 104.375 bits (259), Expect = 1.409e-28
Identity = 50/83 (60.24%), Postives = 58/83 (69.88%), Query Frame = 2
Query:  536 HNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            H C  AGC K+Y K SHLKAH R HTGERPF+C W  C KRF RSDEL +H + HT EK+  C  C K F+RS+ LSKH +TH
Sbjct:    5 HACTYAGCNKIYGKSSHLKAHTRTHTGERPFMCTWKDCEKRFARSDELARHYRTHTGEKRYVCKICDKRFMRSDHLSKHSKTH 87          

HSP 2 Score: 43.8986 bits (102), Expect = 3.963e-6
Identity = 20/58 (34.48%), Postives = 33/58 (56.90%), Query Frame = 2
Query:  632 CNWLFCGKRFNRSDELQKHLKVHTDEKKLNC--PKCGKHFVRSEQLSKHIQTHNKEEK 799
            C +  C K + +S  L+ H + HT E+   C    C K F RS++L++H +TH  E++
Sbjct:    7 CTYAGCNKIYGKSSHLKAHTRTHTGERPFMCTWKDCEKRFARSDELARHYRTHTGEKR 64          
BLAST of sp6-9 vs. Ensembl Medaka
Match: sp8b (transcription factor Sp8 [Source:NCBI gene;Acc:101158882])

HSP 1 Score: 178.333 bits (451), Expect = 1.903e-52
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2
Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  282 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 399          
BLAST of sp6-9 vs. Ensembl Medaka
Match: sp8b (transcription factor Sp8 [Source:NCBI gene;Acc:101158882])

HSP 1 Score: 178.333 bits (451), Expect = 3.457e-52
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2
Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  309 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 426          
BLAST of sp6-9 vs. Ensembl Medaka
Match: sp9 (Sp9 transcription factor [Source:NCBI gene;Acc:101162490])

HSP 1 Score: 171.014 bits (432), Expect = 8.548e-50
Identity = 74/110 (67.27%), Postives = 83/110 (75.45%), Query Frame = 2
Query:  467 AVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            A C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKHI+TH 
Sbjct:  282 ATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHIKTHT 391          
BLAST of sp6-9 vs. Ensembl Medaka
Match: ENSORLT00000004969.2 (Sp8 transcription factor [Source:NCBI gene;Acc:101172989])

HSP 1 Score: 167.933 bits (424), Expect = 2.068e-48
Identity = 73/117 (62.39%), Postives = 87/117 (74.36%), Query Frame = 2
Query:  452 RYSNRAVCSCPNCQEMENLSGYLS---KNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKE 793
            R+S +A C CPNCQE + L    S   +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  C  C K F+RS+ L+KH++TH  E
Sbjct:  295 RHSGKATCDCPNCQEADRLGQGGSSPRRRGVHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFECSVCHKRFMRSDHLNKHVRTHTAE 411          
BLAST of sp6-9 vs. Ensembl Medaka
Match: sp4 (Sp4 transcription factor [Source:NCBI gene;Acc:101167131])

HSP 1 Score: 171.4 bits (433), Expect = 3.535e-48
Identity = 72/117 (61.54%), Postives = 89/117 (76.07%), Query Frame = 2
Query:  464 RAVCSCPNCQEMENL-SGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH-NKEEKSKL 808
            R  CSCPNC++ E   SG  +K   H CH+ GCGK+Y K SHL+AHLRWHTGERPFVCNW+FCGKRF RSDELQ+H + HT EK+  CP+C K F+RS+ LSKHI+TH NK+  + +
Sbjct:  561 RVACSCPNCRDGEGRNSGDPTKKKQHICHMEGCGKVYGKTSHLRAHLRWHTGERPFVCNWIFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHIKTHQNKKSGAAV 677          
BLAST of sp6-9 vs. Planmine SMEST
Match: SMESG000050160.1 (SMESG000050160.1)

HSP 1 Score: 473.011 bits (1216), Expect = 3.304e-171
Identity = 227/228 (99.56%), Postives = 227/228 (99.56%), Query Frame = 2
Query:  134 MNNTAIQNIWRFTFPYHDQKIHQYPSTQSDVNNGFHPFLSLPNYQSLTSSNPYLPSVQNELALNLSDSHILNLPPTPNFDKHTFQSIPANYFNPFISTFYSGNSQPRYSNRAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSKLTSR 817
            MNNTAIQNIWRFTFPYHDQKIHQYPSTQSDVNNGFHPFLS PNYQSLTSSNPYLPSVQNELALNLSDSHILNLPPTPNFDKHTFQSIPANYFNPFISTFYSGNSQPRYSNRAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSKLTSR
Sbjct:    1 MNNTAIQNIWRFTFPYHDQKIHQYPSTQSDVNNGFHPFLSFPNYQSLTSSNPYLPSVQNELALNLSDSHILNLPPTPNFDKHTFQSIPANYFNPFISTFYSGNSQPRYSNRAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSKLTSR 228          
BLAST of sp6-9 vs. Planmine SMEST
Match: SMESG000067314.1 (SMESG000067314.1)

HSP 1 Score: 127.487 bits (319), Expect = 1.419e-34
Identity = 70/161 (43.48%), Postives = 91/161 (56.52%), Query Frame = 2
Query:  413 PFISTFYSGNSQPRYSNRAVCSCPNCQE---MEN-----LSGYLSKNNS-------HNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEK-----------------------KLNCPKCGKHFVRSEQLSKHIQT 781
            P  +  Y  +S   YS+   C CPNCQE   +EN     L   ++ + S       HNCH+ GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+H+++H + K                       KL C  C + + +++ L KHI++
Sbjct:  142 PLSNINYPRSSTRGYSSVGNCECPNCQETPFIENTHQKLLPTIMNASESLGQYPILHNCHVPGCGKVYKKSSHLKAHLRWHTGERPFVCNWLFCGKRFARSDELQQHIRLHQETKDNTNSTALSPKKELKTVDKDLGIKLTCEFCYRSYSKNDLLLKHIKS 302          
BLAST of sp6-9 vs. Planmine SMEST
Match: SMESG000023227.1 (SMESG000023227.1)

HSP 1 Score: 114.775 bits (286), Expect = 3.083e-29
Identity = 54/105 (51.43%), Postives = 68/105 (64.76%), Query Frame = 2
Query:  473 CSCPNCQEMENLSGYL-SKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            C+CPNC +  N    L      H C+I  C K Y K SHLKAH+RWH  +RPFVC +  C K F RSDELQ+H++ HT EK+  CP C K F+RS+ L+KH +TH
Sbjct:  208 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTH 310          
BLAST of sp6-9 vs. Planmine SMEST
Match: SMESG000025482.1 (SMESG000025482.1)

HSP 1 Score: 102.834 bits (255), Expect = 3.934e-26
Identity = 46/92 (50.00%), Postives = 60/92 (65.22%), Query Frame = 2
Query:  524 KNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEK 799
            +  S  C+   C K Y K SHLKAH+R HTG++P++CNW  C +RF RSDEL +H + HT EK  +C  C + F RS+ LSKHI+TH    K
Sbjct:  104 RQKSFKCNYKSCLKAYYKSSHLKAHIRVHTGDKPYICNWENCQRRFTRSDELSRHKRTHTGEKLFSCSICQRKFNRSDHLSKHIKTHKNHSK 195          
BLAST of sp6-9 vs. Planmine SMEST
Match: SMESG000025482.1 (SMESG000025482.1)

HSP 1 Score: 102.064 bits (253), Expect = 4.186e-26
Identity = 46/92 (50.00%), Postives = 60/92 (65.22%), Query Frame = 2
Query:  524 KNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEK 799
            +  S  C+   C K Y K SHLKAH+R HTG++P++CNW  C +RF RSDEL +H + HT EK  +C  C + F RS+ LSKHI+TH    K
Sbjct:   90 RQKSFKCNYKSCLKAYYKSSHLKAHIRVHTGDKPYICNWENCQRRFTRSDELSRHKRTHTGEKLFSCSICQRKFNRSDHLSKHIKTHKNHSK 181          
The following BLAST results are available for this feature:
BLAST of sp6-9 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SP96.523e-5368.70Sp9 transcription factor [Source:HGNC Symbol;Acc:H... [more]
SP87.601e-5266.10Sp8 transcription factor [Source:HGNC Symbol;Acc:H... [more]
SP82.422e-5166.10Sp8 transcription factor [Source:HGNC Symbol;Acc:H... [more]
SP82.934e-5166.10Sp8 transcription factor [Source:HGNC Symbol;Acc:H... [more]
SP42.755e-4763.89Sp4 transcription factor [Source:HGNC Symbol;Acc:H... [more]
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BLAST of sp6-9 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sptf-33.866e-3151.40SP (Specificity Protein) Transcription Factor [So... [more]
sptf-26.963e-3148.54SP (Specificity Protein) Transcription Factor [So... [more]
klf-34.249e-2351.19Kruppel-Like Factor (Zinc finger protein) [Source... [more]
klf-34.601e-2351.19Kruppel-Like Factor (Zinc finger protein) [Source... [more]
klf-21.432e-2244.68Kruppel-Like Factor (Zinc finger protein) [Source... [more]
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BLAST of sp6-9 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Sp11.905e-5367.80gene:FBgn0020378 transcript:FBtr0071381[more]
Sp11.779e-5267.80gene:FBgn0020378 transcript:FBtr0273354[more]
Sp11.795e-5267.80gene:FBgn0020378 transcript:FBtr0310290[more]
Sp12.064e-5267.80gene:FBgn0020378 transcript:FBtr0331335[more]
Spps1.323e-4060.00gene:FBgn0039169 transcript:FBtr0084565[more]
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BLAST of sp6-9 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sp8a1.160e-5364.75sp8 transcription factor a [Source:ZFIN;Acc:ZDB-GE... [more]
sp8a1.190e-5364.75sp8 transcription factor a [Source:ZFIN;Acc:ZDB-GE... [more]
sp8b1.347e-5266.10sp8 transcription factor b [Source:NCBI gene;Acc:3... [more]
sp8b1.347e-5266.10sp8 transcription factor b [Source:NCBI gene;Acc:3... [more]
sp8b1.448e-5266.10sp8 transcription factor b [Source:NCBI gene;Acc:3... [more]
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BLAST of sp6-9 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sp99.848e-5467.80Sp9 transcription factor [Source:NCBI gene;Acc:779... [more]
sp81.122e-5066.10Xenopus tropicalis Sp8 transcription factor (sp8),... [more]
bsdc13.632e-4861.34BSD domain containing 1 [Source:Xenbase;Acc:XB-GEN... [more]
smox2.825e-4759.02spermine oxidase [Source:Xenbase;Acc:XB-GENE-58408... [more]
zp3.21.522e-4658.26zona pellucida glycoprotein 3 (sperm receptor), ge... [more]
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BLAST of sp6-9 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Sp94.117e-5368.70trans-acting transcription factor 9 [Source:MGI Sy... [more]
Sp81.513e-5166.10trans-acting transcription factor 8 [Source:MGI Sy... [more]
Sp45.846e-4864.49trans-acting transcription factor 4 [Source:MGI Sy... [more]
Sp41.295e-4763.89trans-acting transcription factor 4 [Source:MGI Sy... [more]
Sp32.517e-4762.62trans-acting transcription factor 3 [Source:MGI Sy... [more]
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BLAST of sp6-9 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q0VA40|SP9_XENTR5.217e-5367.80Transcription factor Sp9 OS=Xenopus tropicalis OX=... [more]
sp|Q64HY3|SP9_MOUSE2.880e-5268.70Transcription factor Sp9 OS=Mus musculus OX=10090 ... [more]
sp|P0CG40|SP9_HUMAN3.133e-5268.70Transcription factor Sp9 OS=Homo sapiens OX=9606 G... [more]
sp|Q6P0J3|SP8_DANRE1.017e-5166.10Transcription factor Sp8 OS=Danio rerio OX=7955 GN... [more]
sp|Q64HY5|SP8_CHICK5.019e-5165.55Transcription factor Sp8 OS=Gallus gallus OX=9031 ... [more]
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BLAST of sp6-9 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
H9B8J71.188e-16799.56Sp6-9 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1[more]
A0A515MD641.589e-5543.24Sp6-9 (Fragment) OS=Aphonopelma hentzi OX=231250 G... [more]
W4YK022.622e-5551.41Uncharacterized protein OS=Strongylocentrotus purp... [more]
A0A2R4FYF31.510e-5443.75Zinc finger transcription factor Sp6/9 OS=Centruro... [more]
A0A2H4PSJ44.281e-5468.85Zinc finger transcription factor Sp6-9 OS=Parastea... [more]
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BLAST of sp6-9 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sp8a1.754e-5366.95Sp8 transcription factor [Source:NCBI gene;Acc:103... [more]
ENSAMXT00000020600.29.850e-5365.22pep primary_assembly:Astyanax_mexicanus-2.0:10:329... [more]
sp43.896e-4962.93Sp4 transcription factor [Source:NCBI gene;Acc:103... [more]
sp96.216e-4966.97sp9 transcription factor [Source:NCBI gene;Acc:405... [more]
sp3a1.467e-4858.62Sp3 transcription factor [Source:NCBI gene;Acc:103... [more]
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BLAST of sp6-9 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sp41.037e-4663.06sp4 transcription factor [Source:ZFIN;Acc:ZDB-GENE... [more]
sp42.288e-4661.34sp4 transcription factor [Source:ZFIN;Acc:ZDB-GENE... [more]
ENSPMAT00000008916.12.953e-4357.66pep scaffold:Pmarinus_7.0:GL476670:39536:47416:-1 ... [more]
ENSPMAT00000000432.13.744e-4052.34pep scaffold:Pmarinus_7.0:GL477956:42837:43256:-1 ... [more]
sp8b1.632e-3864.95sp8 transcription factor b [Source:NCBI gene;Acc:3... [more]
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BLAST of sp6-9 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
AZF18.167e-1743.59Zinc-finger transcription factor; involved in diau... [more]
CRZ15.714e-1641.18Transcription factor, activates transcription of s... [more]
FZF13.748e-1539.39Transcription factor involved in sulfite metabolis... [more]
ZAP12.629e-1442.53Zinc-regulated transcription factor; binds to zinc... [more]
MIG28.048e-1142.37Zinc finger transcriptional repressor; cooperates ... [more]
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BLAST of sp6-9 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO428852.960e-5357.24Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO429415.666e-4759.82Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO429392.252e-4270.45Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO421193.556e-3753.91Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO322901.409e-2860.24Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of sp6-9 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sp8b1.903e-5266.10transcription factor Sp8 [Source:NCBI gene;Acc:101... [more]
sp8b3.457e-5266.10transcription factor Sp8 [Source:NCBI gene;Acc:101... [more]
sp98.548e-5067.27Sp9 transcription factor [Source:NCBI gene;Acc:101... [more]
ENSORLT00000004969.22.068e-4862.39Sp8 transcription factor [Source:NCBI gene;Acc:101... [more]
sp43.535e-4861.54Sp4 transcription factor [Source:NCBI gene;Acc:101... [more]
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BLAST of sp6-9 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000050160.13.304e-17199.56SMESG000050160.1[more]
SMESG000067314.11.419e-3443.48SMESG000067314.1[more]
SMESG000023227.13.083e-2951.43SMESG000023227.1[more]
SMESG000025482.13.934e-2650.00SMESG000025482.1[more]
SMESG000025482.14.186e-2650.00SMESG000025482.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30011934
GenBankAFC87782.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011934 ID=SMED30011934|Name=sp6-9|organism=Schmidtea mediterranea sexual|type=transcript|length=865bp
TAAAATTTAATCTGAAATCTTTCGAGCTATGGATCTGACTTCAATAAATA
ACGTTGAGAGCAATTATCAATCTTGGCTATTGGAACGCTATAAGTCGGTG
AGTAAAATTAATCCGATCACAAATGAAAATGAAATGAATAATACTGCAAT
TCAAAATATTTGGCGGTTCACATTTCCATACCATGACCAAAAGATCCATC
AGTATCCCTCAACACAAAGTGACGTCAATAATGGGTTTCATCCTTTTTTA
TCCCTTCCTAATTATCAATCGTTAACAAGTTCAAACCCATACTTACCATC
TGTTCAAAATGAGTTGGCGTTGAATTTATCCGATTCACATATTCTAAATC
TCCCTCCAACTCCAAACTTCGATAAACACACTTTCCAGTCTATTCCTGCA
AACTATTTCAATCCGTTCATTTCAACTTTCTACTCTGGAAACTCTCAACC
GAGATATTCAAACCGAGCGGTTTGTAGCTGTCCAAATTGCCAGGAAATGG
AAAATCTATCGGGATATTTATCCAAAAATAATTCCCATAATTGCCATATT
GCCGGATGCGGAAAGTTATATTCAAAACCTAGTCATTTGAAAGCACATCT
TCGATGGCATACTGGTGAAAGGCCATTTGTTTGCAACTGGTTGTTTTGTG
GGAAACGCTTCAATCGGTCCGATGAATTGCAGAAACACCTGAAGGTTCAC
ACAGATGAGAAGAAGCTAAACTGCCCAAAATGTGGGAAACATTTTGTTCG
TTCTGAACAACTGAGTAAACATATTCAAACTCATAATAAAGAGGAAAAAT
CAAAACTAACGAGTCGTTAACAAACAATTGTGAATCTAAATATTATTAGA
AATTTATAGTTGGAC
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protein sequence of SMED30011934-orf-1

>SMED30011934-orf-1 ID=SMED30011934-orf-1|Name=SMED30011934-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=264bp
MDLTSINNVESNYQSWLLERYKSVSKINPITNENEMNNTAIQNIWRFTFP
YHDQKIHQYPSTQSDVNNGFHPFLSLPNYQSLTSSNPYLPSVQNELALNL
SDSHILNLPPTPNFDKHTFQSIPANYFNPFISTFYSGNSQPRYSNRAVCS
CPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPF
VCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQ
THNKEEKSKLTSR*
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Aliases
The feature 'sp6-9' has the following synonyms
Synonym
sp6-9
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000031pigment cup cell
PLANA:0000036eye
PLANA:0000044cephalic ganglia
PLANA:0000095trail cell
PLANA:0000099neuron
PLANA:0000103central nervous system
PLANA:0000143ventral region of the whole animal
PLANA:0000418head
PLANA:0007561head margin
Vocabulary: INTERPRO
TermDefinition
IPR036236Znf_C2H2_sf
IPR015880Znf_C2H2-like
IPR013087Znf_C2H2_type
IPR007087Znf_C2H2
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0046872metal ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013087Zinc finger C2H2-typeSMARTSM00355c2h2final6coord: 230..252
e-value: 0.0034
score: 26.6
coord: 200..224
e-value: 4.1E-4
score: 29.7
coord: 170..194
e-value: 4.7
score: 16.2
IPR013087Zinc finger C2H2-typePFAMPF00096zf-C2H2coord: 200..224
e-value: 2.7E-5
score: 24.4
coord: 231..252
e-value: 2.0E-5
score: 24.8
coord: 170..194
e-value: 0.0013
score: 19.0
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 202..224
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 232..252
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 172..194
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 200..229
score: 14.544
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 230..257
score: 12.612
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 170..199
score: 14.295
NoneNo IPR availableGENE3DG3DSA:3.30.160.60coord: 197..221
e-value: 2.2E-13
score: 51.9
NoneNo IPR availableGENE3DG3DSA:3.30.160.60coord: 163..196
e-value: 1.4E-9
score: 39.7
NoneNo IPR availableGENE3DG3DSA:3.30.160.60coord: 222..255
e-value: 6.8E-8
score: 34.4
NoneNo IPR availablePANTHERPTHR23235TRANSCRIPTION FACTOR SP3coord: 21..257
NoneNo IPR availablePANTHERPTHR23235:SF15SP1, ISOFORM F-RELATEDcoord: 21..257
IPR036236Zinc finger C2H2 superfamilySUPERFAMILYSSF57667beta-beta-alpha zinc fingerscoord: 217..255
IPR036236Zinc finger C2H2 superfamilySUPERFAMILYSSF57667beta-beta-alpha zinc fingerscoord: 184..228