sp6-9

Overview


Name	sp6-9 (AFC87782.1)
Smed ID	SMED30011934
Length (bp)	865

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of sp6-9 (SMED30011934) t-SNE clustered cells

Violin plots show distribution of expression levels for sp6-9 (SMED30011934) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of sp6-9 (SMED30011934) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for sp6-9 (SMED30011934) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30011934

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 17

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
head region	SMED30011934	h1SMcG0005177	SmedASXL_006176	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
head region	SMED30011934	h1SMcG0005177	dd_Smed_v6_17385_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
optic cup	SMED30011934	h1SMcG0005177	JN983830.1	smed_ncbi_20240424	PMID:30194301 Mihaylova et al., 2018
optic cup progenitor cell	SMED30011934	h1SMcG0005177	JN983830.1	smed_ncbi_20240424	PMID:30194301 Mihaylova et al., 2018
eye	SMED30011934	h1SMcG0005177	sp6-9	smed_ncbi_20240424	PMID:25254346 Scimone et al., 2014
central nervous system	SMED30011934	h1SMcG0005177	sp6-9	smed_ncbi_20240424	PMID:25254346 Scimone et al., 2014
eye	SMED30011934	h1SMcG0005177	Contig37509	uc_Smed_v2	PMID:27612384 Roberts-Galbraith et al., 2016
eye	SMED30011934	h1SMcG0005177	Contig37509	newmark_ests	PMID:27612384 Roberts-Galbraith et al., 2016
neuron	SMED30011934	h1SMcG0005177	Contig37509	uc_Smed_v2	PMID:27612384 Roberts-Galbraith et al., 2016
neuron	SMED30011934	h1SMcG0005177	Contig37509	newmark_ests	PMID:27612384 Roberts-Galbraith et al., 2016
pigment cup cell	SMED30011934	h1SMcG0005177	JN983830.1	smed_ncbi_20240424	PMID:28976975 Su et al., 2017
trail cell	SMED30011934	h1SMcG0005177	JN983830.1	smed_ncbi_20240424	PMID:28976975 Su et al., 2017
eye	SMED30011934	h1SMcG0005177	sp6-9	smed_ncbi_20240424	PMID:21852957 Lapan et al., 2011
cephalic ganglia	SMED30011934	h1SMcG0005177	sp6-9	smed_ncbi_20240424	PMID:21852957 Lapan et al., 2011
ventral region of the whole animal	SMED30011934	h1SMcG0005177	sp6-9	smed_ncbi_20240424	PMID:21852957 Lapan et al., 2011
head margin	SMED30011934	h1SMcG0005177	sp6-9	smed_ncbi_20240424	PMID:21852957 Lapan et al., 2011
trail cell	SMED30011934	h1SMcG0005177	sp6-9	smed_ncbi_20240424	PMID:21852957 Lapan et al., 2011

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of sp6-9 vs. Ensembl Human
Match: SP9 (Sp9 transcription factor [Source:HGNC Symbol;Acc:HGNC:30690])

HSP 1 Score: 181.03 bits (458), Expect = 6.523e-53
Identity = 79/115 (68.70%), Postives = 88/115 (76.52%), Query Frame = 2

Query:  452 RYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKHI+THN
Sbjct:  301 RYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHIKTHN 415

[Close]

BLAST of sp6-9 vs. Ensembl Human
Match: SP8 (Sp8 transcription factor [Source:HGNC Symbol;Acc:HGNC:19196])

HSP 1 Score: 177.563 bits (449), Expect = 7.601e-52
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2

Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  297 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 414

[Close]

BLAST of sp6-9 vs. Ensembl Human
Match: SP8 (Sp8 transcription factor [Source:HGNC Symbol;Acc:HGNC:19196])

HSP 1 Score: 177.178 bits (448), Expect = 2.422e-51
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2

Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  339 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 456

[Close]

BLAST of sp6-9 vs. Ensembl Human
Match: SP8 (Sp8 transcription factor [Source:HGNC Symbol;Acc:HGNC:19196])

HSP 1 Score: 176.792 bits (447), Expect = 2.934e-51
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2

Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  321 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 438

[Close]

BLAST of sp6-9 vs. Ensembl Human
Match: SP4 (Sp4 transcription factor [Source:HGNC Symbol;Acc:HGNC:11209])

HSP 1 Score: 169.859 bits (429), Expect = 2.755e-47
Identity = 69/108 (63.89%), Postives = 82/108 (75.93%), Query Frame = 2

Query:  464 RAVCSCPNCQEMENL-SGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            R  CSCPNC+E E   S    K   H CHI GCGK+Y K SHL+AHLRWHTGERPF+CNW+FCGKRF RSDELQ+H + HT EK+  CP+C K F+RS+ LSKH++TH
Sbjct:  605 RVACSCPNCREGEGRGSNEPGKKKQHICHIEGCGKVYGKTSHLRAHLRWHTGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHVKTH 712

[Close]

BLAST of sp6-9 vs. Ensembl Celegans
Match: sptf-3 (SP (Specificity Protein) Transcription Factor [Source:UniProtKB/TrEMBL;Acc:Q9XW26])

HSP 1 Score: 120.168 bits (300), Expect = 3.866e-31
Identity = 55/107 (51.40%), Postives = 70/107 (65.42%), Query Frame = 2

Query:  464 RAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            R  C+CPNC +  N  G   K+  H CH+  C K Y K SHL+AHLR H G +PF C+W  C K+F RSDELQ+H + HT EK+  C  CGK F+RS+ L+KH +TH
Sbjct:  293 RCACTCPNCLQQNNRQGD-GKSRIHICHL--CNKTYGKTSHLRAHLRGHAGNKPFACDWQHCNKKFTRSDELQRHRRTHTGEKRFACNHCGKKFMRSDHLTKHERTH 396

[Close]

BLAST of sp6-9 vs. Ensembl Celegans
Match: sptf-2 (SP (Specificity Protein) Transcription Factor [Source:UniProtKB/TrEMBL;Acc:Q22678])

HSP 1 Score: 113.62 bits (283), Expect = 6.963e-31
Identity = 50/103 (48.54%), Postives = 71/103 (68.93%), Query Frame = 2

Query:  473 CSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQT 781
            C+CPNC+ +++  G     ++H C + GCGK Y K SHL+AHLR HTG+RPFVC+W  CGKRF+RSD+L +H + HT E +  C  C + F RS+ L +H+ +
Sbjct:   58 CTCPNCKAIKH--GDRGSQHTHLCSVPGCGKTYKKTSHLRAHLRKHTGDRPFVCDWFDCGKRFDRSDQLIRHKRTHTKEYRFACKFCIRQFSRSDHLQQHLTS 158

[Close]

BLAST of sp6-9 vs. Ensembl Celegans
Match: klf-3 (Kruppel-Like Factor (Zinc finger protein) [Source:UniProtKB/TrEMBL;Acc:P91329])

HSP 1 Score: 96.6709 bits (239), Expect = 4.249e-23
Identity = 43/84 (51.19%), Postives = 56/84 (66.67%), Query Frame = 2

Query:  536 HNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            H C   GC K YSK SHLKAH R H+GE+PFVC W  C  +F RSDEL +H++ HT +K   C  C ++F RS+ LS H++ H+
Sbjct:  224 HACTYPGCFKKYSKSSHLKAHERTHSGEKPFVCKWQNCSWKFARSDELTRHMRKHTGDKPFRCSLCDRNFARSDHLSLHMKRHS 307

[Close]

BLAST of sp6-9 vs. Ensembl Celegans
Match: klf-3 (Kruppel-Like Factor (Zinc finger protein) [Source:UniProtKB/TrEMBL;Acc:P91329])

HSP 1 Score: 96.6709 bits (239), Expect = 4.601e-23
Identity = 43/84 (51.19%), Postives = 56/84 (66.67%), Query Frame = 2

Query:  536 HNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            H C   GC K YSK SHLKAH R H+GE+PFVC W  C  +F RSDEL +H++ HT +K   C  C ++F RS+ LS H++ H+
Sbjct:  230 HACTYPGCFKKYSKSSHLKAHERTHSGEKPFVCKWQNCSWKFARSDELTRHMRKHTGDKPFRCSLCDRNFARSDHLSLHMKRHS 313

[Close]

BLAST of sp6-9 vs. Ensembl Celegans
Match: klf-2 (Kruppel-Like Factor (Zinc finger protein) [Source:UniProtKB/TrEMBL;Acc:O62259])

HSP 1 Score: 95.1301 bits (235), Expect = 1.432e-22
Identity = 42/94 (44.68%), Postives = 57/94 (60.64%), Query Frame = 2

Query:  518 LSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEK 799
            L +   H C   GCGK+Y+K SHL AH R H+GE+P+ C W  C  RF RSDEL +H + HT  K   C +C + F RS+ L  H++ H  +E+
Sbjct:  192 LDRLRVHKCFYQGCGKVYTKSSHLTAHERVHSGEKPYPCEWPGCSWRFARSDELTRHYRKHTGAKPFACKECSRKFSRSDHLQLHMKRHETDEQ 285

[Close]

BLAST of sp6-9 vs. Ensembl Fly
Match: Sp1 (gene:FBgn0020378 transcript:FBtr0071381)

HSP 1 Score: 182.185 bits (461), Expect = 1.905e-53
Identity = 80/118 (67.80%), Postives = 92/118 (77.97%), Query Frame = 2

Query:  443 SQPRYSNRAVCSCPNCQEMENLSG---YLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            SQ RY+ RA C CPNCQE E L     +L K N H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ L+KH++THN
Sbjct:  156 SQRRYAGRATCDCPNCQEAERLGPAGVHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLAKHVKTHN 273

[Close]

BLAST of sp6-9 vs. Ensembl Fly
Match: Sp1 (gene:FBgn0020378 transcript:FBtr0273354)

HSP 1 Score: 182.956 bits (463), Expect = 1.779e-52
Identity = 80/118 (67.80%), Postives = 92/118 (77.97%), Query Frame = 2

Query:  443 SQPRYSNRAVCSCPNCQEMENLSG---YLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            SQ RY+ RA C CPNCQE E L     +L K N H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ L+KH++THN
Sbjct:  351 SQRRYAGRATCDCPNCQEAERLGPAGVHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLAKHVKTHN 468

[Close]

BLAST of sp6-9 vs. Ensembl Fly
Match: Sp1 (gene:FBgn0020378 transcript:FBtr0310290)

HSP 1 Score: 182.956 bits (463), Expect = 1.795e-52
Identity = 80/118 (67.80%), Postives = 92/118 (77.97%), Query Frame = 2

Query:  443 SQPRYSNRAVCSCPNCQEMENLSG---YLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            SQ RY+ RA C CPNCQE E L     +L K N H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ L+KH++THN
Sbjct:  356 SQRRYAGRATCDCPNCQEAERLGPAGVHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLAKHVKTHN 473

[Close]

BLAST of sp6-9 vs. Ensembl Fly
Match: Sp1 (gene:FBgn0020378 transcript:FBtr0331335)

HSP 1 Score: 182.956 bits (463), Expect = 2.064e-52
Identity = 80/118 (67.80%), Postives = 92/118 (77.97%), Query Frame = 2

Query:  443 SQPRYSNRAVCSCPNCQEMENLSG---YLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            SQ RY+ RA C CPNCQE E L     +L K N H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ L+KH++THN
Sbjct:  351 SQRRYAGRATCDCPNCQEAERLGPAGVHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLAKHVKTHN 468

[Close]

BLAST of sp6-9 vs. Ensembl Fly
Match: Spps (gene:FBgn0039169 transcript:FBtr0084565)

HSP 1 Score: 150.599 bits (379), Expect = 1.323e-40
Identity = 69/115 (60.00%), Postives = 82/115 (71.30%), Query Frame = 2

Query:  446 QPRYSNRAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNK 790
            +PR   R  C+CPNC + E  S    K   H CHI GC K+Y K SHL+AHLRWHTGERPFVC+W FCGKRF RSDELQ+H + HT EK+  C +C K F+RS+ LSKHI+TH K
Sbjct:  714 KPRL-KRVACTCPNCTDGEKHS---DKKRQHICHITGCHKVYGKTSHLRAHLRWHTGERPFVCSWAFCGKRFTRSDELQRHRRTHTGEKRFQCQECNKKFMRSDHLSKHIKTHFK 824

[Close]

BLAST of sp6-9 vs. Ensembl Zebrafish
Match: sp8a (sp8 transcription factor a [Source:ZFIN;Acc:ZDB-GENE-030131-9849])

HSP 1 Score: 180.644 bits (457), Expect = 1.160e-53
Identity = 79/122 (64.75%), Postives = 92/122 (75.41%), Query Frame = 2

Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEE 796
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+ +CP C K F+RS+ LSKH++TH+  E
Sbjct:  245 SSTRRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFSCPVCNKRFMRSDHLSKHVKTHSAGE 366

[Close]

BLAST of sp6-9 vs. Ensembl Zebrafish
Match: sp8a (sp8 transcription factor a [Source:ZFIN;Acc:ZDB-GENE-030131-9849])

HSP 1 Score: 179.874 bits (455), Expect = 1.190e-53
Identity = 79/122 (64.75%), Postives = 92/122 (75.41%), Query Frame = 2

Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEE 796
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+ +CP C K F+RS+ LSKH++TH+  E
Sbjct:  227 SSTRRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFSCPVCNKRFMRSDHLSKHVKTHSAGE 348

[Close]

BLAST of sp6-9 vs. Ensembl Zebrafish
Match: sp8b (sp8 transcription factor b [Source:NCBI gene;Acc:324931])

HSP 1 Score: 178.333 bits (451), Expect = 1.347e-52
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2

Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  262 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 379

[Close]

Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  262 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 379

[Close]

BLAST of sp6-9 vs. Ensembl Zebrafish
Match: sp8b (sp8 transcription factor b [Source:NCBI gene;Acc:324931])

HSP 1 Score: 178.718 bits (452), Expect = 1.448e-52
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2

Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  280 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 397

[Close]

BLAST of sp6-9 vs. Ensembl Xenopus
Match: sp9 (Sp9 transcription factor [Source:NCBI gene;Acc:779722])

HSP 1 Score: 181.415 bits (459), Expect = 9.848e-54
Identity = 80/118 (67.80%), Postives = 89/118 (75.42%), Query Frame = 2

Query:  443 SQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKHI+THN
Sbjct:  261 SARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHIKTHN 378

[Close]

BLAST of sp6-9 vs. Ensembl Xenopus
Match: sp8 (Xenopus tropicalis Sp8 transcription factor (sp8), mRNA. [Source:RefSeq mRNA;Acc:NM_001292062])

HSP 1 Score: 175.637 bits (444), Expect = 1.122e-50
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2

Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  350 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 467

[Close]

BLAST of sp6-9 vs. Ensembl Xenopus
Match: bsdc1 (BSD domain containing 1 [Source:Xenbase;Acc:XB-GENE-5892016])

HSP 1 Score: 167.162 bits (422), Expect = 3.632e-48
Identity = 73/119 (61.34%), Postives = 87/119 (73.11%), Query Frame = 2

Query:  458 SNRAVCSCPNCQEMENL---SGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSK 805
            S R  C CPNCQE+E L   +  L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDEL++H++ HT EKK  CP C K F RS+ LSKH ++H  +  +K
Sbjct:  262 SGRPSCDCPNCQELERLGASAASLKRKPVHSCHIPGCGKVYGKASHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELERHVRTHTREKKFTCPLCAKRFTRSDHLSKHQRSHVDQPGAK 380

[Close]

BLAST of sp6-9 vs. Ensembl Xenopus
Match: smox (spermine oxidase [Source:Xenbase;Acc:XB-GENE-5840847])

HSP 1 Score: 162.925 bits (411), Expect = 2.825e-47
Identity = 72/122 (59.02%), Postives = 88/122 (72.13%), Query Frame = 2

Query:  443 SQPRYSNRAVCSCPNCQEMENLSGYLSK---NNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEK 799
            + PR + +  C CPNCQE E + G        N HNCHI GCGK Y+K SHLKAHLRWH+G+RPFVCNWLFCGKRF RSDELQ+HL+ HT  KK  CP C + F+RS+ L+KH++TH  E +
Sbjct:  195 AMPRSAAQTACRCPNCQEAERVGGGGEGGKHKNLHNCHIPGCGKAYAKTSHLKAHLRWHSGDRPFVCNWLFCGKRFTRSDELQRHLQTHTGAKKYPCPSCSRVFMRSDHLTKHMKTHEGESR 316

[Close]

BLAST of sp6-9 vs. Ensembl Xenopus
Match: zp3.2 (zona pellucida glycoprotein 3 (sperm receptor), gene 2 [Source:Xenbase;Acc:XB-GENE-1019608])

HSP 1 Score: 166.777 bits (421), Expect = 1.522e-46
Identity = 67/115 (58.26%), Postives = 83/115 (72.17%), Query Frame = 2

Query:  440 NSQPRYSNRAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            N + +   R  C+CPNC++       L K   H CHI GCGK+Y K SHL+AHLRWH+GERPFVC W+FCGKRF RSDELQ+H + HT EKK  CP+C K F+RS+ L+KHI+TH
Sbjct:  473 NQEGKRLRRVACTCPNCKDGAGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCTWMFCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTH 587

[Close]

BLAST of sp6-9 vs. Ensembl Mouse
Match: Sp9 (trans-acting transcription factor 9 [Source:MGI Symbol;Acc:MGI:3574660])

HSP 1 Score: 181.03 bits (458), Expect = 4.117e-53
Identity = 79/115 (68.70%), Postives = 88/115 (76.52%), Query Frame = 2

Query:  452 RYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKHI+THN
Sbjct:  301 RYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHIKTHN 415

[Close]

BLAST of sp6-9 vs. Ensembl Mouse
Match: Sp8 (trans-acting transcription factor 8 [Source:MGI Symbol;Acc:MGI:2443471])

HSP 1 Score: 176.792 bits (447), Expect = 1.513e-51
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2

Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  321 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 438

[Close]

BLAST of sp6-9 vs. Ensembl Mouse
Match: Sp4 (trans-acting transcription factor 4 [Source:MGI Symbol;Acc:MGI:107595])

HSP 1 Score: 171.4 bits (433), Expect = 5.846e-48
Identity = 69/107 (64.49%), Postives = 82/107 (76.64%), Query Frame = 2

Query:  464 RAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            R  CSCPNC+E E  S    K   H CHI GCGK+Y K SHL+AHLRWHTGERPF+CNW+FCGKRF RSDELQ+H + HT EK+  CP+C K F+RS+ LSKH++TH
Sbjct:  620 RVACSCPNCREGEG-SSEPGKKKQHVCHIEGCGKVYGKTSHLRAHLRWHTGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHVKTH 725

[Close]

BLAST of sp6-9 vs. Ensembl Mouse
Match: Sp4 (trans-acting transcription factor 4 [Source:MGI Symbol;Acc:MGI:107595])

HSP 1 Score: 170.629 bits (431), Expect = 1.295e-47
Identity = 69/108 (63.89%), Postives = 82/108 (75.93%), Query Frame = 2

Query:  464 RAVCSCPNCQEMENL-SGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            R  CSCPNC+E E   S    K   H CHI GCGK+Y K SHL+AHLRWHTGERPF+CNW+FCGKRF RSDELQ+H + HT EK+  CP+C K F+RS+ LSKH++TH
Sbjct:  620 RVACSCPNCREGEGRGSSEPGKKKQHVCHIEGCGKVYGKTSHLRAHLRWHTGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHVKTH 727

[Close]

BLAST of sp6-9 vs. Ensembl Mouse
Match: Sp3 (trans-acting transcription factor 3 [Source:MGI Symbol;Acc:MGI:1277166])

HSP 1 Score: 169.474 bits (428), Expect = 2.517e-47
Identity = 67/107 (62.62%), Postives = 81/107 (75.70%), Query Frame = 2

Query:  464 RAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            R  C+CPNC+E       L K   H CHI GCGK+Y K SHL+AHLRWH+GERPF+CNW+FCGKRF RSDELQ+H + HT EKK  CP+C K F+RS+ L+KHI+TH
Sbjct:  555 RVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTH 661

[Close]

BLAST of sp6-9 vs. UniProt/SwissProt
Match: sp|Q0VA40|SP9_XENTR (Transcription factor Sp9 OS=Xenopus tropicalis OX=8364 GN=sp9 PE=2 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 5.217e-53
Identity = 80/118 (67.80%), Postives = 89/118 (75.42%), Query Frame = 2

Query:  443 SQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKHI+THN
Sbjct:  261 SARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHIKTHN 378

[Close]

BLAST of sp6-9 vs. UniProt/SwissProt
Match: sp|Q64HY3|SP9_MOUSE (Transcription factor Sp9 OS=Mus musculus OX=10090 GN=Sp9 PE=1 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 2.880e-52
Identity = 79/115 (68.70%), Postives = 88/115 (76.52%), Query Frame = 2

Query:  452 RYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKHI+THN
Sbjct:  301 RYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHIKTHN 415

[Close]

BLAST of sp6-9 vs. UniProt/SwissProt
Match: sp|P0CG40|SP9_HUMAN (Transcription factor Sp9 OS=Homo sapiens OX=9606 GN=SP9 PE=3 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 3.133e-52
Identity = 79/115 (68.70%), Postives = 88/115 (76.52%), Query Frame = 2

Query:  452 RYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKHI+THN
Sbjct:  301 RYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHIKTHN 415

[Close]

BLAST of sp6-9 vs. UniProt/SwissProt
Match: sp|Q6P0J3|SP8_DANRE (Transcription factor Sp8 OS=Danio rerio OX=7955 GN=sp8b PE=2 SV=1)

HSP 1 Score: 178.333 bits (451), Expect = 1.017e-51
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2

Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  262 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 379

[Close]

BLAST of sp6-9 vs. UniProt/SwissProt
Match: sp|Q64HY5|SP8_CHICK (Transcription factor Sp8 OS=Gallus gallus OX=9031 GN=SP8 PE=2 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 5.019e-51
Identity = 78/119 (65.55%), Postives = 90/119 (75.63%), Query Frame = 2

Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH+
Sbjct:  307 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTHS 425

[Close]

BLAST of sp6-9 vs. TrEMBL
Match: H9B8J7 (Sp6-9 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 473.011 bits (1216), Expect = 1.188e-167
Identity = 227/228 (99.56%), Postives = 227/228 (99.56%), Query Frame = 2

Query:  134 MNNTAIQNIWRFTFPYHDQKIHQYPSTQSDVNNGFHPFLSLPNYQSLTSSNPYLPSVQNELALNLSDSHILNLPPTPNFDKHTFQSIPANYFNPFISTFYSGNSQPRYSNRAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSKLTSR 817
            MNNTAIQNIWRFTFPYHDQKIHQYPSTQSDVNNGFHPFLS PNYQSLTSSNPYLPSVQNELALNLSDSHILNLPPTPNFDKHTFQSIPANYFNPFISTFYSGNSQPRYSNRAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSKLTSR
Sbjct:    1 MNNTAIQNIWRFTFPYHDQKIHQYPSTQSDVNNGFHPFLSFPNYQSLTSSNPYLPSVQNELALNLSDSHILNLPPTPNFDKHTFQSIPANYFNPFISTFYSGNSQPRYSNRAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSKLTSR 228

[Close]

BLAST of sp6-9 vs. TrEMBL
Match: A0A515MD64 (Sp6-9 (Fragment) OS=Aphonopelma hentzi OX=231250 GN=Sp6-9 PE=2 SV=1)

HSP 1 Score: 194.897 bits (494), Expect = 1.589e-55
Identity = 112/259 (43.24%), Postives = 140/259 (54.05%), Query Frame = 2

Query:   50 NVESNYQSWLLERYKSVSKINPITNENEMNNTAIQNIW----RFTFPYHDQKIHQYPSTQSDVNNGFHPFLSLPNYQSLTSSNPYLPSVQNELALNLSDSHILNLPPTPNFDKHTFQSIPANYFNPFISTFYSGNSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSK 805
            NV     + +     SV+ +N  T+  +M+  +    W      T   H Q    YPS+    + G  P L+  N   L SS  +L          L D++   LP           + P +   P +    S  SQ RYS RA C CPNCQE E L     +L K N H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++THN    +K
Sbjct:  152 NVAGATHTGIKTEVSSVNTLN-STSWWDMHTASSAGSWLDMSGATTGLHSQLPANYPSS----DYGLGPSLAASNTAHLLSSGQHL----------LQDTYKSMLPSAQGVGPSV--ASPFSLSQPTLPQMPSPRSQRRYSGRATCDCPNCQEAERLGPAGAHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPICNKRFMRSDHLSKHVKTHNDNGGTK 393

[Close]

BLAST of sp6-9 vs. TrEMBL
Match: W4YK02 (Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 2.622e-55
Identity = 91/177 (51.41%), Postives = 115/177 (64.97%), Query Frame = 2

Query:  263 YQSLTSSNPYLPSVQNELALNLSDSHILNLPPTPNFDKHTFQSIPANYFNPFISTFYSGNSQPRYSNRAVCSCPNCQEMENLSGY---LSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            Y  LT++NP+L S       +  +S +      P +D  +   + +++F P         +  RYS RA C CPNCQE E L+     L K N H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+ +CP C K F+RS+ LSKH++TH
Sbjct:   22 YPPLTTTNPHLFSPSQLFPPSSYNSFL-----PPGYDIMSLTEMRSSFFGPAGLGLSLSRAGRRYSGRATCDCPNCQENERLAAAGQPLRKKNVHSCHIPGCGKIYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFSCPTCNKRFMRSDHLSKHVKTH 193

[Close]

BLAST of sp6-9 vs. TrEMBL
Match: A0A2R4FYF3 (Zinc finger transcription factor Sp6/9 OS=Centruroides sculpturatus OX=218467 GN=Sp6 PE=2 SV=1)

HSP 1 Score: 192.971 bits (489), Expect = 1.510e-54
Identity = 112/256 (43.75%), Postives = 141/256 (55.08%), Query Frame = 2

Query:   50 NVESNYQSWLLERYKSVSKINPITNENEMNNTAIQNIW----RFTFPYHDQKIHQYPSTQSDVNNGFHPFLSLPNYQSLTSSNPYLPSVQNELALNLSDSHILNLPPTPNFDKHTFQSIPANYFN---PFISTFYSGNSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            NV     S +     +V+ +N  T+  +M+  +    W      T   H Q    YPS++     G  P L+  N   L SS  +L          L D++   LP        + Q + A+ F    P +    S  SQ RY+ RA C CPNCQE E L     +L K N H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++THN
Sbjct:  170 NVAGAAHSGIKPEVSTVNSLN-STSWWDMHAASGAGSWLDMSGATSGLHSQLPTNYPSSE----YGLAPTLAASNTAHLLSSGQHL----------LQDTYKSMLP--------SAQGVGASPFGLSQPALPQMPSPRSQRRYTGRATCDCPNCQEAERLGPAGAHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPICNKRFMRSDHLSKHVKTHN 402

[Close]

BLAST of sp6-9 vs. TrEMBL
Match: A0A2H4PSJ4 (Zinc finger transcription factor Sp6-9 OS=Parasteatoda tepidariorum OX=114398 PE=2 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 4.281e-54
Identity = 84/122 (68.85%), Postives = 95/122 (77.87%), Query Frame = 2

Query:  443 SQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEK 799
            SQ RYS RA C CPNCQE E L     +L K N H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP CGK F+RS+ L+KH++THN  EK
Sbjct:  272 SQRRYSGRATCDCPNCQEAERLGPAGAHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFVCPNCGKRFMRSDHLAKHVKTHNGSEK 393

[Close]

BLAST of sp6-9 vs. Ensembl Cavefish
Match: sp8a (Sp8 transcription factor [Source:NCBI gene;Acc:103039368])

HSP 1 Score: 181.03 bits (458), Expect = 1.754e-53
Identity = 79/118 (66.95%), Postives = 90/118 (76.27%), Query Frame = 2

Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP CGK F+RS+ LSKH++TH
Sbjct:  291 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCGKRFMRSDHLSKHVKTH 408

[Close]

BLAST of sp6-9 vs. Ensembl Cavefish
Match: ENSAMXT00000020600.2 (pep primary_assembly:Astyanax_mexicanus-2.0:10:32985937:32991833:-1 gene:ENSAMXG00000020011.2 transcript:ENSAMXT00000020600.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 178.718 bits (452), Expect = 9.850e-53
Identity = 75/115 (65.22%), Postives = 89/115 (77.39%), Query Frame = 2

Query:  455 YSNRAVCSCPNCQEMENL----SGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            Y+ RA C CPNCQE E L    +  + +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP CGK F+RS+ LSKH++TH+
Sbjct:  287 YAGRATCDCPNCQEAERLGPAGAASMRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCGKRFMRSDHLSKHVKTHS 401

[Close]

BLAST of sp6-9 vs. Ensembl Cavefish
Match: sp4 (Sp4 transcription factor [Source:NCBI gene;Acc:103039672])

HSP 1 Score: 174.096 bits (440), Expect = 3.896e-49
Identity = 73/116 (62.93%), Postives = 89/116 (76.72%), Query Frame = 2

Query:  464 RAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH-NKEEKSKL 808
            R  CSCPNC++ E  +   SK   H CH+AGCGK+Y K SHL+AHLRWHTGERPFVCNW+FCGKRF RSDELQ+H + HT EK+  CP+C K F+RS+ LSKHI+TH NK+  + L
Sbjct:  594 RVACSCPNCRDGEGRNSDPSKKKQHVCHMAGCGKVYGKTSHLRAHLRWHTGERPFVCNWIFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHIKTHQNKKGGTAL 709

[Close]

BLAST of sp6-9 vs. Ensembl Cavefish
Match: sp9 (sp9 transcription factor [Source:NCBI gene;Acc:405896])

HSP 1 Score: 168.703 bits (426), Expect = 6.216e-49
Identity = 73/109 (66.97%), Postives = 82/109 (75.23%), Query Frame = 2

Query:  470 VCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
             C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKHI+TH 
Sbjct:  285 TCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHIKTHT 393

[Close]

BLAST of sp6-9 vs. Ensembl Cavefish
Match: sp3a (Sp3 transcription factor [Source:NCBI gene;Acc:103031127])

HSP 1 Score: 171.785 bits (434), Expect = 1.467e-48
Identity = 68/116 (58.62%), Postives = 84/116 (72.41%), Query Frame = 2

Query:  464 RAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSKLT 811
            R  C+CPNC+E       + K   H CHI GCGK+Y K SHL+AHLRWH+GERPFVCNW+FCGKRF RSDELQ+H + HT EKK  CP+C K F+RS+ L+KHI+TH  ++    T
Sbjct:  489 RVACTCPNCKEGGGRGTSIGKKKQHVCHIVGCGKVYGKTSHLRAHLRWHSGERPFVCNWMFCGKRFTRSDELQRHRRTHTGEKKFVCPQCSKRFMRSDHLAKHIKTHQNKKGGGGT 604

[Close]

BLAST of sp6-9 vs. Ensembl Sea Lamprey
Match: sp4 (sp4 transcription factor [Source:ZFIN;Acc:ZDB-GENE-031202-3])

HSP 1 Score: 159.844 bits (403), Expect = 1.037e-46
Identity = 70/111 (63.06%), Postives = 80/111 (72.07%), Query Frame = 2

Query:  461 NRAVCSCPNCQEMENL-SGYL-SKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
             R  C+CPNC++ E   SG    K   H CHI GC K+Y K SHL+AHLRWHTGERPFVC WLFCGKRF RSDELQ+H + HT EKK  CP C K F+RS+ LSKHI+TH 
Sbjct:  267 RRVACACPNCRDGEGRRSGSEPGKKKQHVCHIPGCSKVYGKTSHLRAHLRWHTGERPFVCGWLFCGKRFTRSDELQRHRRTHTGEKKFPCPHCSKRFMRSDHLSKHIRTHQ 377

[Close]

BLAST of sp6-9 vs. Ensembl Sea Lamprey
Match: sp4 (sp4 transcription factor [Source:ZFIN;Acc:ZDB-GENE-031202-3])

HSP 1 Score: 160.614 bits (405), Expect = 2.288e-46
Identity = 73/119 (61.34%), Postives = 85/119 (71.43%), Query Frame = 2

Query:  464 RAVCSCPNCQEMENL-SGYL-SKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSKLTS 814
            R  C+CPNC++ E   SG    K   H CHI GC K+Y K SHL+AHLRWHTGERPFVC WLFCGKRF RSDELQ+H + HT EKK  CP C K F+RS+ LSKHI+TH + +KS L  
Sbjct:  312 RVACACPNCRDGEGRRSGSEPGKKKQHVCHIPGCSKVYGKTSHLRAHLRWHTGERPFVCGWLFCGKRFTRSDELQRHRRTHTGEKKFPCPHCSKRFMRSDHLSKHIRTH-QNKKSGLVG 429

[Close]

BLAST of sp6-9 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008916.1 (pep scaffold:Pmarinus_7.0:GL476670:39536:47416:-1 gene:ENSPMAG00000008063.1 transcript:ENSPMAT00000008916.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 155.221 bits (391), Expect = 2.953e-43
Identity = 64/111 (57.66%), Postives = 81/111 (72.97%), Query Frame = 2

Query:  464 RAVCSCPNCQEMEN-LSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKE 793
            R  C CPNC++ E  ++G   +   H CHI GC K+Y K SHL+AHLRWH+GERPFVC WL CGKRF RSDELQ+H + HT EKK  C +C K F+RS+ LSKH++TH+ +
Sbjct:  561 RVPCMCPNCRDSEGKVTGEAGQKKQHICHITGCSKVYGKTSHLRAHLRWHSGERPFVCTWLHCGKRFTRSDELQRHKRTHTGEKKFICSECSKRFMRSDHLSKHMKTHSAK 671

[Close]

BLAST of sp6-9 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000432.1 (pep scaffold:Pmarinus_7.0:GL477956:42837:43256:-1 gene:ENSPMAG00000000389.1 transcript:ENSPMAT00000000432.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 134.806 bits (338), Expect = 3.744e-40
Identity = 56/107 (52.34%), Postives = 71/107 (66.36%), Query Frame = 2

Query:  473 CSCPNCQEMENLSGYLSKNN-SHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNK 790
            C CPNCQ   N +   S +   H CH++GCGK Y K SHL+AHLRWH GERP+ C W  C +RF RSDEL++H + HT E++  C  CGK F RS+ L KH +TH +
Sbjct:   16 CRCPNCQTASNTATISSSSRRQHTCHVSGCGKAYGKTSHLRAHLRWHAGERPYACAWPLCARRFTRSDELRRHARTHTGERRFACVACGKRFARSDHLGKHARTHRE 122

[Close]

BLAST of sp6-9 vs. Ensembl Sea Lamprey
Match: sp8b (sp8 transcription factor b [Source:NCBI gene;Acc:324931])

HSP 1 Score: 135.576 bits (340), Expect = 1.632e-38
Identity = 63/97 (64.95%), Postives = 67/97 (69.07%), Query Frame = 2

Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQ---KHLKVHTDEK 712
             S  R+  RA C CPNCQE E L      L +   HNCHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ   KH K H+  K
Sbjct:  189 GSSRRFPGRATCDCPNCQEAERLGPAGASLRRKGLHNCHIPGCGKVYGKSSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQHLNKHAKTHSGGK 285

[Close]

BLAST of sp6-9 vs. Ensembl Yeast
Match: AZF1 (Zinc-finger transcription factor; involved in diauxic shift; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity; relocalizes to the cytosol in response to hypoxia [Source:SGD;Acc:S000005639])

HSP 1 Score: 78.5666 bits (192), Expect = 8.167e-17
Identity = 34/78 (43.59%), Postives = 50/78 (64.10%), Query Frame = 2

Query:  557 CGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNK 790
            C +L+S+ +HL+ H+R H G +PFVC++  CGKRF +   L+ H ++HT EK  +C  C K F R   L+ H+ TH K
Sbjct:  598 CHRLFSQATHLEVHVRSHIGYKPFVCDY--CGKRFTQGGNLRTHERLHTGEKPYSCDICDKKFSRKGNLAAHLVTHQK 673

HSP 2 Score: 56.9954 bits (136), Expect = 1.071e-9
Identity = 30/89 (33.71%), Postives = 46/89 (51.69%), Query Frame = 2

Query:  557 CGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNC--PKCGKHFVRSEQLSKHIQTHNKEEKSKLTSR 817
            CGK +++  +L+ H R HTGE+P+ C+   C K+F+R   L  HL  H   K   C    C K F +   +  H    +KE  + LT++
Sbjct:  626 CGKRFTQGGNLRTHERLHTGEKPYSCD--ICDKKFSRKGNLAAHLVTHQKLKPFVCKLENCNKTFTQLGNMKAHQNRFHKETLNALTAK 712

[Close]

BLAST of sp6-9 vs. Ensembl Yeast
Match: CRZ1 (Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress; can be activated in stochastic pulses of nuclear localization in response to calcium [Source:SGD;Acc:S000004972])

HSP 1 Score: 75.8702 bits (185), Expect = 5.714e-16
Identity = 35/85 (41.18%), Postives = 51/85 (60.00%), Query Frame = 2

Query:  557 CGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCP---------KCGKHFVRSEQLSKHIQTH 784
            CGK +++P +LK+HLR HT ERPF+C+   CGK F R  + ++H  +HT +K+  C           CGK F RS+ L +H +T 
Sbjct:  574 CGKKFTRPYNLKSHLRTHTNERPFICS--ICGKAFARQHDRKRHEDLHTGKKRYVCGGKLKDGKPWGCGKKFARSDALGRHFKTE 656

HSP 2 Score: 46.9802 bits (110), Expect = 2.186e-6
Identity = 21/58 (36.21%), Postives = 31/58 (53.45%), Query Frame = 2

Query:  626 FVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEK 799
            F C+   CGK+F R   L+ HL+ HT+E+   C  CGK F R     +H   H  +++
Sbjct:  569 FACD--VCGKKFTRPYNLKSHLRTHTNERPFICSICGKAFARQHDRKRHEDLHTGKKR 624

[Close]

BLAST of sp6-9 vs. Ensembl Yeast
Match: FZF1 (Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000003223])

HSP 1 Score: 72.4034 bits (176), Expect = 3.748e-15
Identity = 39/99 (39.39%), Postives = 59/99 (59.60%), Query Frame = 2

Query:  521 SKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSKLTSR 817
            +K+ ++ C   GC K+Y++PS L+ H   HT ++P+ C+   CGK+F R   L+ H   H+  K   C  C K FV ++QL +H+ +H  E KSKL SR
Sbjct:    7 TKSRNYKCSFDGCEKVYNRPSLLQQHQNSHTNQKPYHCDEPGCGKKFIRPCHLRVHKWTHSQIKPKACTLCQKRFVTNQQLRRHLNSH--ERKSKLASR 103

[Close]

BLAST of sp6-9 vs. Ensembl Yeast
Match: ZAP1 (Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains [Source:SGD;Acc:S000003592])

HSP 1 Score: 71.2478 bits (173), Expect = 2.629e-14
Identity = 37/87 (42.53%), Postives = 50/87 (57.47%), Query Frame = 2

Query:  557 CGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSKLTSR 817
            C + +S    L  H R H+GE+P+ C+   C K+F  S  L+ H++ HT EK L C  CGK F  S  LSKHI+TH K+ K    S+
Sbjct:  773 CKRCFSSEETLVQHTRTHSGEKPYKCH--ICNKKFAISSSLKIHIRTHTGEKPLQCKICGKRFNESSNLSKHIKTHQKKYKCSDCSK 857

HSP 2 Score: 63.1586 bits (152), Expect = 9.684e-12
Identity = 34/68 (50.00%), Postives = 40/68 (58.82%), Query Frame = 2

Query:  542 CHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHF 745
            CHI  C K ++  S LK H+R HTGE+P  C    CGKRFN S  L KH+K H  +KK  C  C K F
Sbjct:  798 CHI--CNKKFAISSSLKIHIRTHTGEKPLQCK--ICGKRFNESSNLSKHIKTH--QKKYKCSDCSKSF 859

HSP 3 Score: 51.6026 bits (122), Expect = 7.564e-8
Identity = 29/88 (32.95%), Postives = 42/88 (47.73%), Query Frame = 2

Query:  542 CHIAGCGKLYSKPSHLKAHLR---WHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEE 796
            C   GC K +S    L  HL       G+  + C W  C + F +  +L +HLKVH+  K   C  C + F   E L +H +TH+ E+
Sbjct:  707 CQWDGCNKSFSSAQELNDHLEAVHLTRGKSEYQCLWHDCHRTFPQRQKLIRHLKVHSKYKPYKCKTCKRCFSSEETLVQHTRTHSGEK 794

[Close]

BLAST of sp6-9 vs. Ensembl Yeast
Match: MIG2 (Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced gene repression; under low glucose conditions relocalizes to mitochondrion, where it interacts with Ups1p, antagonizes mitochondrial fission factor Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth in response to glucose depletion; activated in stochastic pulses of nuclear localization in response to low glucose [Source:SGD;Acc:S000003177])

HSP 1 Score: 60.077 bits (144), Expect = 8.048e-11
Identity = 25/59 (42.37%), Postives = 37/59 (62.71%), Query Frame = 2

Query:  557 CGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKC 733
            C + + +  H K HLR HTGE+P  C +  CGK F+RSDEL++H++ HT + +    K 
Sbjct:   22 CHRGFHRLEHKKRHLRTHTGEKPHHCAFPGCGKSFSRSDELKRHMRTHTGQSQRRLKKA 80

HSP 2 Score: 55.4546 bits (132), Expect = 2.538e-9
Identity = 26/65 (40.00%), Postives = 42/65 (64.62%), Query Frame = 2

Query:  620 RPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNC--PKCGKHFVRSEQLSKHIQTHNKEEKSKL 808
            RPF C+   C + F+R +  ++HL+ HT EK  +C  P CGK F RS++L +H++TH  + + +L
Sbjct:   15 RPFRCDT--CHRGFHRLEHKKRHLRTHTGEKPHHCAFPGCGKSFSRSDELKRHMRTHTGQSQRRL 77

[Close]

BLAST of sp6-9 vs. Ensembl Nematostella
Match: EDO42885 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S016])

HSP 1 Score: 177.178 bits (448), Expect = 2.960e-53
Identity = 83/145 (57.24%), Postives = 99/145 (68.28%), Query Frame = 2

Query:  380 TFQSIPANYFNPFISTFYSGN------SQPRYSNRAVCSCPNCQEMENLSGY---LSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            T+   PA+YF P  + F          +  RY+ RA C CPNCQ+ E +S       K + H CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++THN
Sbjct:  210 TYIDGPASYF-PGTAAFLQSTPSLVPRTTRRYTGRATCDCPNCQDNERMSASGAPFRKKSQHICHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPICSKRFMRSDHLSKHVKTHN 353

[Close]

BLAST of sp6-9 vs. Ensembl Nematostella
Match: EDO42941 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S019])

HSP 1 Score: 152.91 bits (385), Expect = 5.666e-47
Identity = 67/112 (59.82%), Postives = 84/112 (75.00%), Query Frame = 2

Query:  452 RYSNRAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            R   R  C+CPNC++ E  +    K   H CH+ GCGK+Y K SHL+AHLRWH+GERPFVCNWLFCGKRF RSDELQ+H + HT EK+  CP+C K F+RS+ LSKH++TH+
Sbjct:    5 RRLRRIACTCPNCRDGEGRTANGRKQ--HVCHVPGCGKVYGKTSHLRAHLRWHSGERPFVCNWLFCGKRFTRSDELQRHRRTHTGEKRFTCPECNKKFMRSDHLSKHVKTHS 114

[Close]

BLAST of sp6-9 vs. Ensembl Nematostella
Match: EDO42939 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S017])

HSP 1 Score: 140.198 bits (352), Expect = 2.252e-42
Identity = 62/88 (70.45%), Postives = 70/88 (79.55%), Query Frame = 2

Query:  521 SKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            SK   H CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGK F RSDELQ+HL+ HT EK+  C +CGK F+RS+ L KH +TH
Sbjct:    3 SKRKQHVCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKSFTRSDELQRHLRTHTGEKRFQCDECGKRFMRSDHLRKHQKTH 90

HSP 2 Score: 46.2098 bits (108), Expect = 6.854e-7
Identity = 23/59 (38.98%), Postives = 37/59 (62.71%), Query Frame = 2

Query:  629 VCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPK--CGKHFVRSEQLSKHIQTHNKEEK 799
            VC+   CGK + ++  L+ HL+ HT E+   C    CGK F RS++L +H++TH  E++
Sbjct:    9 VCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKSFTRSDELQRHLRTHTGEKR 67

[Close]

BLAST of sp6-9 vs. Ensembl Nematostella
Match: EDO42119 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2C5])

HSP 1 Score: 128.257 bits (321), Expect = 3.556e-37
Identity = 62/115 (53.91%), Postives = 75/115 (65.22%), Query Frame = 2

Query:  464 RAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWL-----FCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKE 793
            + +C+CPNC    N +   +    HNCH  GCGK+Y K SHLKAHLRWH GERPFVCNW       CGK F RSDELQ+HL+ HT EK   C  CGK F RS+ L+KH++ H  +
Sbjct:    1 KCLCACPNCVSGINSNPSPTHPKYHNCHYPGCGKIYGKTSHLKAHLRWHRGERPFVCNWQTILGSTCGKSFTRSDELQRHLRTHTKEKAHTCHVCGKAFGRSDHLTKHLRIHKAK 115

[Close]

BLAST of sp6-9 vs. Ensembl Nematostella
Match: EDO32290 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SVG8])

HSP 1 Score: 104.375 bits (259), Expect = 1.409e-28
Identity = 50/83 (60.24%), Postives = 58/83 (69.88%), Query Frame = 2

Query:  536 HNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            H C  AGC K+Y K SHLKAH R HTGERPF+C W  C KRF RSDEL +H + HT EK+  C  C K F+RS+ LSKH +TH
Sbjct:    5 HACTYAGCNKIYGKSSHLKAHTRTHTGERPFMCTWKDCEKRFARSDELARHYRTHTGEKRYVCKICDKRFMRSDHLSKHSKTH 87

HSP 2 Score: 43.8986 bits (102), Expect = 3.963e-6
Identity = 20/58 (34.48%), Postives = 33/58 (56.90%), Query Frame = 2

Query:  632 CNWLFCGKRFNRSDELQKHLKVHTDEKKLNC--PKCGKHFVRSEQLSKHIQTHNKEEK 799
            C +  C K + +S  L+ H + HT E+   C    C K F RS++L++H +TH  E++
Sbjct:    7 CTYAGCNKIYGKSSHLKAHTRTHTGERPFMCTWKDCEKRFARSDELARHYRTHTGEKR 64

[Close]

BLAST of sp6-9 vs. Ensembl Medaka
Match: sp8b (transcription factor Sp8 [Source:NCBI gene;Acc:101158882])

HSP 1 Score: 178.333 bits (451), Expect = 1.903e-52
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2

Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  282 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 399

[Close]

BLAST of sp6-9 vs. Ensembl Medaka
Match: sp8b (transcription factor Sp8 [Source:NCBI gene;Acc:101158882])

HSP 1 Score: 178.333 bits (451), Expect = 3.457e-52
Identity = 78/118 (66.10%), Postives = 89/118 (75.42%), Query Frame = 2

Query:  440 NSQPRYSNRAVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            +S  RYS RA C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKH++TH
Sbjct:  309 SSARRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTH 426

[Close]

BLAST of sp6-9 vs. Ensembl Medaka
Match: sp9 (Sp9 transcription factor [Source:NCBI gene;Acc:101162490])

HSP 1 Score: 171.014 bits (432), Expect = 8.548e-50
Identity = 74/110 (67.27%), Postives = 83/110 (75.45%), Query Frame = 2

Query:  467 AVCSCPNCQEMENLS---GYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHN 787
            A C CPNCQE E L      L +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  CP C K F+RS+ LSKHI+TH 
Sbjct:  282 ATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHIKTHT 391

[Close]

BLAST of sp6-9 vs. Ensembl Medaka
Match: ENSORLT00000004969.2 (Sp8 transcription factor [Source:NCBI gene;Acc:101172989])

HSP 1 Score: 167.933 bits (424), Expect = 2.068e-48
Identity = 73/117 (62.39%), Postives = 87/117 (74.36%), Query Frame = 2

Query:  452 RYSNRAVCSCPNCQEMENLSGYLS---KNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKE 793
            R+S +A C CPNCQE + L    S   +   H+CHI GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+HL+ HT EK+  C  C K F+RS+ L+KH++TH  E
Sbjct:  295 RHSGKATCDCPNCQEADRLGQGGSSPRRRGVHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFECSVCHKRFMRSDHLNKHVRTHTAE 411

[Close]

BLAST of sp6-9 vs. Ensembl Medaka
Match: sp4 (Sp4 transcription factor [Source:NCBI gene;Acc:101167131])

HSP 1 Score: 171.4 bits (433), Expect = 3.535e-48
Identity = 72/117 (61.54%), Postives = 89/117 (76.07%), Query Frame = 2

Query:  464 RAVCSCPNCQEMENL-SGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH-NKEEKSKL 808
            R  CSCPNC++ E   SG  +K   H CH+ GCGK+Y K SHL+AHLRWHTGERPFVCNW+FCGKRF RSDELQ+H + HT EK+  CP+C K F+RS+ LSKHI+TH NK+  + +
Sbjct:  561 RVACSCPNCRDGEGRNSGDPTKKKQHICHMEGCGKVYGKTSHLRAHLRWHTGERPFVCNWIFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHIKTHQNKKSGAAV 677

[Close]

BLAST of sp6-9 vs. Planmine SMEST
Match: SMESG000050160.1 (SMESG000050160.1)

HSP 1 Score: 473.011 bits (1216), Expect = 3.304e-171
Identity = 227/228 (99.56%), Postives = 227/228 (99.56%), Query Frame = 2

Query:  134 MNNTAIQNIWRFTFPYHDQKIHQYPSTQSDVNNGFHPFLSLPNYQSLTSSNPYLPSVQNELALNLSDSHILNLPPTPNFDKHTFQSIPANYFNPFISTFYSGNSQPRYSNRAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSKLTSR 817
            MNNTAIQNIWRFTFPYHDQKIHQYPSTQSDVNNGFHPFLS PNYQSLTSSNPYLPSVQNELALNLSDSHILNLPPTPNFDKHTFQSIPANYFNPFISTFYSGNSQPRYSNRAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSKLTSR
Sbjct:    1 MNNTAIQNIWRFTFPYHDQKIHQYPSTQSDVNNGFHPFLSFPNYQSLTSSNPYLPSVQNELALNLSDSHILNLPPTPNFDKHTFQSIPANYFNPFISTFYSGNSQPRYSNRAVCSCPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEKSKLTSR 228

[Close]

BLAST of sp6-9 vs. Planmine SMEST
Match: SMESG000067314.1 (SMESG000067314.1)

HSP 1 Score: 127.487 bits (319), Expect = 1.419e-34
Identity = 70/161 (43.48%), Postives = 91/161 (56.52%), Query Frame = 2

Query:  413 PFISTFYSGNSQPRYSNRAVCSCPNCQE---MEN-----LSGYLSKNNS-------HNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEK-----------------------KLNCPKCGKHFVRSEQLSKHIQT 781
            P  +  Y  +S   YS+   C CPNCQE   +EN     L   ++ + S       HNCH+ GCGK+Y K SHLKAHLRWHTGERPFVCNWLFCGKRF RSDELQ+H+++H + K                       KL C  C + + +++ L KHI++
Sbjct:  142 PLSNINYPRSSTRGYSSVGNCECPNCQETPFIENTHQKLLPTIMNASESLGQYPILHNCHVPGCGKVYKKSSHLKAHLRWHTGERPFVCNWLFCGKRFARSDELQQHIRLHQETKDNTNSTALSPKKELKTVDKDLGIKLTCEFCYRSYSKNDLLLKHIKS 302

[Close]

BLAST of sp6-9 vs. Planmine SMEST
Match: SMESG000023227.1 (SMESG000023227.1)

HSP 1 Score: 114.775 bits (286), Expect = 3.083e-29
Identity = 54/105 (51.43%), Postives = 68/105 (64.76%), Query Frame = 2

Query:  473 CSCPNCQEMENLSGYL-SKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 784
            C+CPNC +  N    L      H C+I  C K Y K SHLKAH+RWH  +RPFVC +  C K F RSDELQ+H++ HT EK+  CP C K F+RS+ L+KH +TH
Sbjct:  208 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTH 310

[Close]

BLAST of sp6-9 vs. Planmine SMEST
Match: SMESG000025482.1 (SMESG000025482.1)

HSP 1 Score: 102.834 bits (255), Expect = 3.934e-26
Identity = 46/92 (50.00%), Postives = 60/92 (65.22%), Query Frame = 2

Query:  524 KNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEK 799
            +  S  C+   C K Y K SHLKAH+R HTG++P++CNW  C +RF RSDEL +H + HT EK  +C  C + F RS+ LSKHI+TH    K
Sbjct:  104 RQKSFKCNYKSCLKAYYKSSHLKAHIRVHTGDKPYICNWENCQRRFTRSDELSRHKRTHTGEKLFSCSICQRKFNRSDHLSKHIKTHKNHSK 195

[Close]

BLAST of sp6-9 vs. Planmine SMEST
Match: SMESG000025482.1 (SMESG000025482.1)

HSP 1 Score: 102.064 bits (253), Expect = 4.186e-26
Identity = 46/92 (50.00%), Postives = 60/92 (65.22%), Query Frame = 2

Query:  524 KNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTHNKEEK 799
            +  S  C+   C K Y K SHLKAH+R HTG++P++CNW  C +RF RSDEL +H + HT EK  +C  C + F RS+ LSKHI+TH    K
Sbjct:   90 RQKSFKCNYKSCLKAYYKSSHLKAHIRVHTGDKPYICNWENCQRRFTRSDELSRHKRTHTGEKLFSCSICQRKFNRSDHLSKHIKTHKNHSK 181

The following BLAST results are available for this feature:

BLAST of sp6-9 vs. Ensembl Human
BLAST of sp6-9 vs. Ensembl Celegans
BLAST of sp6-9 vs. Ensembl Fly
BLAST of sp6-9 vs. Ensembl Zebrafish
BLAST of sp6-9 vs. Ensembl Xenopus
BLAST of sp6-9 vs. Ensembl Mouse
BLAST of sp6-9 vs. UniProt/SwissProt
BLAST of sp6-9 vs. TrEMBL
BLAST of sp6-9 vs. Ensembl Cavefish
BLAST of sp6-9 vs. Ensembl Sea Lamprey
BLAST of sp6-9 vs. Ensembl Yeast
BLAST of sp6-9 vs. Ensembl Nematostella
BLAST of sp6-9 vs. Ensembl Medaka
BLAST of sp6-9 vs. Planmine SMEST

BLAST of sp6-9 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
SP9	6.523e-53	68.70	Sp9 transcription factor [Source:HGNC Symbol;Acc:H...	[more]
SP8	7.601e-52	66.10	Sp8 transcription factor [Source:HGNC Symbol;Acc:H...	[more]
SP8	2.422e-51	66.10	Sp8 transcription factor [Source:HGNC Symbol;Acc:H...	[more]
SP8	2.934e-51	66.10	Sp8 transcription factor [Source:HGNC Symbol;Acc:H...	[more]
SP4	2.755e-47	63.89	Sp4 transcription factor [Source:HGNC Symbol;Acc:H...	[more]

BLAST of sp6-9 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
sptf-3	3.866e-31	51.40	SP (Specificity Protein) Transcription Factor [So...	[more]
sptf-2	6.963e-31	48.54	SP (Specificity Protein) Transcription Factor [So...	[more]
klf-3	4.249e-23	51.19	Kruppel-Like Factor (Zinc finger protein) [Source...	[more]
klf-3	4.601e-23	51.19	Kruppel-Like Factor (Zinc finger protein) [Source...	[more]
klf-2	1.432e-22	44.68	Kruppel-Like Factor (Zinc finger protein) [Source...	[more]

BLAST of sp6-9 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Sp1	1.905e-53	67.80	gene:FBgn0020378 transcript:FBtr0071381	[more]
Sp1	1.779e-52	67.80	gene:FBgn0020378 transcript:FBtr0273354	[more]
Sp1	1.795e-52	67.80	gene:FBgn0020378 transcript:FBtr0310290	[more]
Sp1	2.064e-52	67.80	gene:FBgn0020378 transcript:FBtr0331335	[more]
Spps	1.323e-40	60.00	gene:FBgn0039169 transcript:FBtr0084565	[more]

BLAST of sp6-9 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
sp8a	1.160e-53	64.75	sp8 transcription factor a [Source:ZFIN;Acc:ZDB-GE...	[more]
sp8a	1.190e-53	64.75	sp8 transcription factor a [Source:ZFIN;Acc:ZDB-GE...	[more]
sp8b	1.347e-52	66.10	sp8 transcription factor b [Source:NCBI gene;Acc:3...	[more]
sp8b	1.347e-52	66.10	sp8 transcription factor b [Source:NCBI gene;Acc:3...	[more]
sp8b	1.448e-52	66.10	sp8 transcription factor b [Source:NCBI gene;Acc:3...	[more]

BLAST of sp6-9 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
sp9	9.848e-54	67.80	Sp9 transcription factor [Source:NCBI gene;Acc:779...	[more]
sp8	1.122e-50	66.10	Xenopus tropicalis Sp8 transcription factor (sp8),...	[more]
bsdc1	3.632e-48	61.34	BSD domain containing 1 [Source:Xenbase;Acc:XB-GEN...	[more]
smox	2.825e-47	59.02	spermine oxidase [Source:Xenbase;Acc:XB-GENE-58408...	[more]
zp3.2	1.522e-46	58.26	zona pellucida glycoprotein 3 (sperm receptor), ge...	[more]

BLAST of sp6-9 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Sp9	4.117e-53	68.70	trans-acting transcription factor 9 [Source:MGI Sy...	[more]
Sp8	1.513e-51	66.10	trans-acting transcription factor 8 [Source:MGI Sy...	[more]
Sp4	5.846e-48	64.49	trans-acting transcription factor 4 [Source:MGI Sy...	[more]
Sp4	1.295e-47	63.89	trans-acting transcription factor 4 [Source:MGI Sy...	[more]
Sp3	2.517e-47	62.62	trans-acting transcription factor 3 [Source:MGI Sy...	[more]

BLAST of sp6-9 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q0VA40\|SP9_XENTR	5.217e-53	67.80	Transcription factor Sp9 OS=Xenopus tropicalis OX=...	[more]
sp\|Q64HY3\|SP9_MOUSE	2.880e-52	68.70	Transcription factor Sp9 OS=Mus musculus OX=10090 ...	[more]
sp\|P0CG40\|SP9_HUMAN	3.133e-52	68.70	Transcription factor Sp9 OS=Homo sapiens OX=9606 G...	[more]
sp\|Q6P0J3\|SP8_DANRE	1.017e-51	66.10	Transcription factor Sp8 OS=Danio rerio OX=7955 GN...	[more]
sp\|Q64HY5\|SP8_CHICK	5.019e-51	65.55	Transcription factor Sp8 OS=Gallus gallus OX=9031 ...	[more]

BLAST of sp6-9 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
H9B8J7	1.188e-167	99.56	Sp6-9 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1	[more]
A0A515MD64	1.589e-55	43.24	Sp6-9 (Fragment) OS=Aphonopelma hentzi OX=231250 G...	[more]
W4YK02	2.622e-55	51.41	Uncharacterized protein OS=Strongylocentrotus purp...	[more]
A0A2R4FYF3	1.510e-54	43.75	Zinc finger transcription factor Sp6/9 OS=Centruro...	[more]
A0A2H4PSJ4	4.281e-54	68.85	Zinc finger transcription factor Sp6-9 OS=Parastea...	[more]

BLAST of sp6-9 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
sp8a	1.754e-53	66.95	Sp8 transcription factor [Source:NCBI gene;Acc:103...	[more]
ENSAMXT00000020600.2	9.850e-53	65.22	pep primary_assembly:Astyanax_mexicanus-2.0:10:329...	[more]
sp4	3.896e-49	62.93	Sp4 transcription factor [Source:NCBI gene;Acc:103...	[more]
sp9	6.216e-49	66.97	sp9 transcription factor [Source:NCBI gene;Acc:405...	[more]
sp3a	1.467e-48	58.62	Sp3 transcription factor [Source:NCBI gene;Acc:103...	[more]

BLAST of sp6-9 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
sp4	1.037e-46	63.06	sp4 transcription factor [Source:ZFIN;Acc:ZDB-GENE...	[more]
sp4	2.288e-46	61.34	sp4 transcription factor [Source:ZFIN;Acc:ZDB-GENE...	[more]
ENSPMAT00000008916.1	2.953e-43	57.66	pep scaffold:Pmarinus_7.0:GL476670:39536:47416:-1 ...	[more]
ENSPMAT00000000432.1	3.744e-40	52.34	pep scaffold:Pmarinus_7.0:GL477956:42837:43256:-1 ...	[more]
sp8b	1.632e-38	64.95	sp8 transcription factor b [Source:NCBI gene;Acc:3...	[more]

BLAST of sp6-9 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5


Match Name	E-value	Identity	Description
AZF1	8.167e-17	43.59	Zinc-finger transcription factor; involved in diau...	[more]
CRZ1	5.714e-16	41.18	Transcription factor, activates transcription of s...	[more]
FZF1	3.748e-15	39.39	Transcription factor involved in sulfite metabolis...	[more]
ZAP1	2.629e-14	42.53	Zinc-regulated transcription factor; binds to zinc...	[more]
MIG2	8.048e-11	42.37	Zinc finger transcriptional repressor; cooperates ...	[more]

BLAST of sp6-9 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO42885	2.960e-53	57.24	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO42941	5.666e-47	59.82	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO42939	2.252e-42	70.45	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO42119	3.556e-37	53.91	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO32290	1.409e-28	60.24	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of sp6-9 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
sp8b	1.903e-52	66.10	transcription factor Sp8 [Source:NCBI gene;Acc:101...	[more]
sp8b	3.457e-52	66.10	transcription factor Sp8 [Source:NCBI gene;Acc:101...	[more]
sp9	8.548e-50	67.27	Sp9 transcription factor [Source:NCBI gene;Acc:101...	[more]
ENSORLT00000004969.2	2.068e-48	62.39	Sp8 transcription factor [Source:NCBI gene;Acc:101...	[more]
sp4	3.535e-48	61.54	Sp4 transcription factor [Source:NCBI gene;Acc:101...	[more]

BLAST of sp6-9 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000050160.1	3.304e-171	99.56	SMESG000050160.1	[more]
SMESG000067314.1	1.419e-34	43.48	SMESG000067314.1	[more]
SMESG000023227.1	3.083e-29	51.43	SMESG000023227.1	[more]
SMESG000025482.1	3.934e-26	50.00	SMESG000025482.1	[more]
SMESG000025482.1	4.186e-26	50.00	SMESG000025482.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Cross References

External references for this transcript


Database	Accession
SmedGD GBrowse	SMED30011934
GenBank	AFC87782.1

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30011934 ID=SMED30011934|Name=sp6-9|organism=Schmidtea mediterranea sexual|type=transcript|length=865bp
TAAAATTTAATCTGAAATCTTTCGAGCTATGGATCTGACTTCAATAAATA
ACGTTGAGAGCAATTATCAATCTTGGCTATTGGAACGCTATAAGTCGGTG
AGTAAAATTAATCCGATCACAAATGAAAATGAAATGAATAATACTGCAAT
TCAAAATATTTGGCGGTTCACATTTCCATACCATGACCAAAAGATCCATC
AGTATCCCTCAACACAAAGTGACGTCAATAATGGGTTTCATCCTTTTTTA
TCCCTTCCTAATTATCAATCGTTAACAAGTTCAAACCCATACTTACCATC
TGTTCAAAATGAGTTGGCGTTGAATTTATCCGATTCACATATTCTAAATC
TCCCTCCAACTCCAAACTTCGATAAACACACTTTCCAGTCTATTCCTGCA
AACTATTTCAATCCGTTCATTTCAACTTTCTACTCTGGAAACTCTCAACC
GAGATATTCAAACCGAGCGGTTTGTAGCTGTCCAAATTGCCAGGAAATGG
AAAATCTATCGGGATATTTATCCAAAAATAATTCCCATAATTGCCATATT
GCCGGATGCGGAAAGTTATATTCAAAACCTAGTCATTTGAAAGCACATCT
TCGATGGCATACTGGTGAAAGGCCATTTGTTTGCAACTGGTTGTTTTGTG
GGAAACGCTTCAATCGGTCCGATGAATTGCAGAAACACCTGAAGGTTCAC
ACAGATGAGAAGAAGCTAAACTGCCCAAAATGTGGGAAACATTTTGTTCG
TTCTGAACAACTGAGTAAACATATTCAAACTCATAATAAAGAGGAAAAAT
CAAAACTAACGAGTCGTTAACAAACAATTGTGAATCTAAATATTATTAGA
AATTTATAGTTGGAC

protein sequence of SMED30011934-orf-1

>SMED30011934-orf-1 ID=SMED30011934-orf-1|Name=SMED30011934-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=264bp
MDLTSINNVESNYQSWLLERYKSVSKINPITNENEMNNTAIQNIWRFTFP
YHDQKIHQYPSTQSDVNNGFHPFLSLPNYQSLTSSNPYLPSVQNELALNL
SDSHILNLPPTPNFDKHTFQSIPANYFNPFISTFYSGNSQPRYSNRAVCS
CPNCQEMENLSGYLSKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPF
VCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQ
THNKEEKSKLTSR*

Aliases

The feature 'sp6-9' has the following synonyms


Synonym
sp6-9

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000031	pigment cup cell
PLANA:0000036	eye
PLANA:0000044	cephalic ganglia
PLANA:0000095	trail cell
PLANA:0000099	neuron
PLANA:0000103	central nervous system
PLANA:0000143	ventral region of the whole animal
PLANA:0000418	head
PLANA:0007561	head margin

Vocabulary: **INTERPRO**
Term	Definition
IPR036236	Znf_C2H2_sf
IPR015880	Znf_C2H2-like
IPR013087	Znf_C2H2_type
IPR007087	Znf_C2H2

Vocabulary: **molecular function**
Term	Definition
GO:0003676	nucleic acid binding
GO:0046872	metal ion binding

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013087	Zinc finger C2H2-type	SMART	SM00355	c2h2final6	coord: 230..252 e-value: 0.0034 score: 26.6 coord: 200..224 e-value: 4.1E-4 score: 29.7 coord: 170..194 e-value: 4.7 score: 16.2
IPR013087	Zinc finger C2H2-type	PFAM	PF00096	zf-C2H2	coord: 200..224 e-value: 2.7E-5 score: 24.4 coord: 231..252 e-value: 2.0E-5 score: 24.8 coord: 170..194 e-value: 0.0013 score: 19.0
IPR013087	Zinc finger C2H2-type	PROSITE	PS00028	ZINC_FINGER_C2H2_1	coord: 202..224
IPR013087	Zinc finger C2H2-type	PROSITE	PS00028	ZINC_FINGER_C2H2_1	coord: 232..252
IPR013087	Zinc finger C2H2-type	PROSITE	PS00028	ZINC_FINGER_C2H2_1	coord: 172..194
IPR013087	Zinc finger C2H2-type	PROSITE	PS50157	ZINC_FINGER_C2H2_2	coord: 200..229 score: 14.544
IPR013087	Zinc finger C2H2-type	PROSITE	PS50157	ZINC_FINGER_C2H2_2	coord: 230..257 score: 12.612
IPR013087	Zinc finger C2H2-type	PROSITE	PS50157	ZINC_FINGER_C2H2_2	coord: 170..199 score: 14.295
None	No IPR available	GENE3D	G3DSA:3.30.160.60		coord: 197..221 e-value: 2.2E-13 score: 51.9
None	No IPR available	GENE3D	G3DSA:3.30.160.60		coord: 163..196 e-value: 1.4E-9 score: 39.7
None	No IPR available	GENE3D	G3DSA:3.30.160.60		coord: 222..255 e-value: 6.8E-8 score: 34.4
None	No IPR available	PANTHER	PTHR23235	TRANSCRIPTION FACTOR SP3	coord: 21..257
None	No IPR available	PANTHER	PTHR23235:SF15	SP1, ISOFORM F-RELATED	coord: 21..257
IPR036236	Zinc finger C2H2 superfamily	SUPERFAMILY	SSF57667	beta-beta-alpha zinc fingers	coord: 217..255
IPR036236	Zinc finger C2H2 superfamily	SUPERFAMILY	SSF57667	beta-beta-alpha zinc fingers	coord: 184..228