SMED30011848

Overview
NameSMED30011848
Smed IDSMED30011848
Length (bp)3679
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30011848 (SMED30011848) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30011848 (SMED30011848) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30011848 (SMED30011848) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30011848 (SMED30011848) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30011848

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30011848h1SMcG0021788 dd_Smed_v4_11248_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30011848h1SMcG0021788 dd_Smed_v4_11248_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30011848h1SMcG0021788 dd_Smed_v4_11248_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30011848h1SMcG0021788 dd_Smed_v4_11248_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30011848h1SMcG0021788 dd_Smed_v4_11248_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30011848h1SMcG0021788 dd_Smed_v4_11248_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of SMED30011848 vs. Planmine SMEST
Match: SMESG000081465.1 (SMESG000081465.1)

HSP 1 Score: 965.681 bits (2495), Expect = 0.000e+0
Identity = 566/568 (99.65%), Postives = 567/568 (99.82%), Query Frame = 1 
Query:   70 MIKFILSDNEQYFHKFNCDISIKENLNDFCKEKGLVPDIYEVYDPIEDNALDCNKRLLDYNLNSLHLRRVNSQFSSEKSSQDLLNFTDTSDKLWNDQSIRTYSQNSYNQIQMLDNNAHKKIKKGFMYQLKKYMPKKDKNMSRSFSESLESPVETEMITLSTDEIYQGSLTTEIXXXXXXXXXXXXXXXXXXXXXXXXTNVLTFYFEENSDSKLQSIDIEIPLPPVDSFELGEHINYYPWQSCTECYTGQCYEDESVYSIISEDPLPLSPGFRVFETPRCPTPKEMSDDGRESPDHTYSTIVKNNKFDKNIDKSDISASSSFNEKDQYSTIAKIQDKKIFYGENVELSNNRSSQESFHVQELSISDNITIFSAPXXXXXXXXXXXXXXXXXXXXEKQKDCESQHHKENCILTFSPIKYKTNIDEDILINNKQYSTNFNFKTEETNFPSNVEHKNNNDLILPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFRNLDETDSKEFLKSNPPQVFSKPRVKINNAEKKDTQTAKLNHKPKNSLIQPIYDDGKMRIEKQGQSLFFSVN 1773
            MIKFILSDNEQYFHKFNCDISIKENLNDFCKEKGLVPDIYEVYDPIEDNALDCNKRLLDYNLNSLHLRRVNSQFSSEKSSQDLLN TDTSDKLWNDQSIRTYSQNSYNQIQMLDNNAHKKIKKGFMYQLKKYMPKKDKNMSRSFSESLESPVETEMITLSTDEIYQGSLTTEIIQRDIQKIQKNNKKKKRNAPAPPKTNVLTFYFEENSDSKLQSIDIEIPLPPVDSFELGEHINYYPWQSCTECYTGQCYEDESVYSIISEDPLPLSPGFRVFETPRCPTPKEMSDDGRESPDHTYSTIVKNNKFDKNIDKSDISASSSFNE+DQYSTIAKIQDKKIFYGENVELSNNRSSQESFHVQELSISDNITIFSAPIISIDSDGSSISSFNNDSVSEKQKDCESQHHKENCILTFSPIKYKTNIDEDILINNKQYSTNFNFKTEETNFPSNVEHKNNNDLILPSIEISSSSEQNISIQSNTLQNNISNEQNNSRNANTFRNLDETDSKEFLKSNPPQVFSKPRVKINNAEKKDTQTAKLNHKPKNSLIQPIYDDGKMRIEKQGQSLFFSVN
Sbjct:    1 MIKFILSDNEQYFHKFNCDISIKENLNDFCKEKGLVPDIYEVYDPIEDNALDCNKRLLDYNLNSLHLRRVNSQFSSEKSSQDLLNLTDTSDKLWNDQSIRTYSQNSYNQIQMLDNNAHKKIKKGFMYQLKKYMPKKDKNMSRSFSESLESPVETEMITLSTDEIYQGSLTTEIIQRDIQKIQKNNKKKKRNAPAPPKTNVLTFYFEENSDSKLQSIDIEIPLPPVDSFELGEHINYYPWQSCTECYTGQCYEDESVYSIISEDPLPLSPGFRVFETPRCPTPKEMSDDGRESPDHTYSTIVKNNKFDKNIDKSDISASSSFNERDQYSTIAKIQDKKIFYGENVELSNNRSSQESFHVQELSISDNITIFSAPIISIDSDGSSISSFNNDSVSEKQKDCESQHHKENCILTFSPIKYKTNIDEDILINNKQYSTNFNFKTEETNFPSNVEHKNNNDLILPSIEISSSSEQNISIQSNTLQNNISNEQNNSRNANTFRNLDETDSKEFLKSNPPQVFSKPRVKINNAEKKDTQTAKLNHKPKNSLIQPIYDDGKMRIEKQGQSLFFSVN 568
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BLAST of SMED30011848 vs. Planmine SMEST
Match: SMESG000081465.1 (SMESG000081465.1)

HSP 1 Score: 963.755 bits (2490), Expect = 0.000e+0
Identity = 565/567 (99.65%), Postives = 566/567 (99.82%), Query Frame = 1 
Query:   70 MIKFILSDNEQYFHKFNCDISIKENLNDFCKEKGLVPDIYEVYDPIEDNALDCNKRLLDYNLNSLHLRRVNSQFSSEKSSQDLLNFTDTSDKLWNDQSIRTYSQNSYNQIQMLDNNAHKKIKKGFMYQLKKYMPKKDKNMSRSFSESLESPVETEMITLSTDEIYQGSLTTEIXXXXXXXXXXXXXXXXXXXXXXXXTNVLTFYFEENSDSKLQSIDIEIPLPPVDSFELGEHINYYPWQSCTECYTGQCYEDESVYSIISEDPLPLSPGFRVFETPRCPTPKEMSDDGRESPDHTYSTIVKNNKFDKNIDKSDISASSSFNEKDQYSTIAKIQDKKIFYGENVELSNNRSSQESFHVQELSISDNITIFSAPXXXXXXXXXXXXXXXXXXXXEKQKDCESQHHKENCILTFSPIKYKTNIDEDILINNKQYSTNFNFKTEETNFPSNVEHKNNNDLILPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFRNLDETDSKEFLKSNPPQVFSKPRVKINNAEKKDTQTAKLNHKPKNSLIQPIYDDGKMRIEKQGQSLFFSV 1770
            MIKFILSDNEQYFHKFNCDISIKENLNDFCKEKGLVPDIYEVYDPIEDNALDCNKRLLDYNLNSLHLRRVNSQFSSEKSSQDLLN TDTSDKLWNDQSIRTYSQNSYNQIQMLDNNAHKKIKKGFMYQLKKYMPKKDKNMSRSFSESLESPVETEMITLSTDEIYQGSLTTEIIQRDIQKIQKNNKKKKRNAPAPPKTNVLTFYFEENSDSKLQSIDIEIPLPPVDSFELGEHINYYPWQSCTECYTGQCYEDESVYSIISEDPLPLSPGFRVFETPRCPTPKEMSDDGRESPDHTYSTIVKNNKFDKNIDKSDISASSSFNE+DQYSTIAKIQDKKIFYGENVELSNNRSSQESFHVQELSISDNITIFSAPIISIDSDGSSISSFNNDSVSEKQKDCESQHHKENCILTFSPIKYKTNIDEDILINNKQYSTNFNFKTEETNFPSNVEHKNNNDLILPSIEISSSSEQNISIQSNTLQNNISNEQNNSRNANTFRNLDETDSKEFLKSNPPQVFSKPRVKINNAEKKDTQTAKLNHKPKNSLIQPIYDDGKMRIEKQGQSLFFSV
Sbjct:    1 MIKFILSDNEQYFHKFNCDISIKENLNDFCKEKGLVPDIYEVYDPIEDNALDCNKRLLDYNLNSLHLRRVNSQFSSEKSSQDLLNLTDTSDKLWNDQSIRTYSQNSYNQIQMLDNNAHKKIKKGFMYQLKKYMPKKDKNMSRSFSESLESPVETEMITLSTDEIYQGSLTTEIIQRDIQKIQKNNKKKKRNAPAPPKTNVLTFYFEENSDSKLQSIDIEIPLPPVDSFELGEHINYYPWQSCTECYTGQCYEDESVYSIISEDPLPLSPGFRVFETPRCPTPKEMSDDGRESPDHTYSTIVKNNKFDKNIDKSDISASSSFNERDQYSTIAKIQDKKIFYGENVELSNNRSSQESFHVQELSISDNITIFSAPIISIDSDGSSISSFNNDSVSEKQKDCESQHHKENCILTFSPIKYKTNIDEDILINNKQYSTNFNFKTEETNFPSNVEHKNNNDLILPSIEISSSSEQNISIQSNTLQNNISNEQNNSRNANTFRNLDETDSKEFLKSNPPQVFSKPRVKINNAEKKDTQTAKLNHKPKNSLIQPIYDDGKMRIEKQGQSLFFSV 567
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BLAST of SMED30011848 vs. Planmine SMEST
Match: SMESG000032032.1 (SMESG000032032.1)

HSP 1 Score: 77.7962 bits (190), Expect = 2.600e-18
Identity = 39/50 (78.00%), Postives = 43/50 (86.00%), Query Frame = 1 
Query: 2413 MKLFNHLSMISQSVKLLFWLILVSTYSSHFETQQTHFQIQTVGTYSTIKL 2562
            MKLFNHLSMISQSV  LFWLI+VSTYS+  ET +T F+IQTVGTYS IKL
Sbjct:    1 MKLFNHLSMISQSVTNLFWLIIVSTYSNKLETHRTLFKIQTVGTYSNIKL 50

HSP 2 Score: 35.039 bits (79), Expect = 2.600e-18
Identity = 15/16 (93.75%), Postives = 15/16 (93.75%), Query Frame = 3 
Query: 2679 NWPTIECKVQLIKSTY 2726
            NWPTIE KVQLIKSTY
Sbjct:   87 NWPTIEYKVQLIKSTY 102
[Close]
BLAST of SMED30011848 vs. Planmine SMEST
Match: SMESG000069530.1 (SMESG000069530.1)

HSP 1 Score: 80.4925 bits (197), Expect = 3.076e-16
Identity = 42/69 (60.87%), Postives = 55/69 (79.71%), Query Frame = -2 
Query: 1999 FNNIRFNIGKTISRAGVFSSMSYQSRFD-VREVLDNEL-KKGYKNLINNFVPIQRFFPRCIKIIHI*GD 2199
            F+ I+FNI K+ISRAG  SSM++QSRFD +++ LDNEL K+ Y++L N F  I+RFFP+CIK IHI GD
Sbjct:  180 FSKIKFNIRKSISRAGGLSSMNFQSRFDFLKDALDNELVKEDYRDLTNYFWHIRRFFPKCIKKIHIFGD 248
[Close]
BLAST of SMED30011848 vs. Planmine SMEST
Match: SMESG000041908.1 (SMESG000041908.1)

HSP 1 Score: 63.929 bits (154), Expect = 8.425e-10
Identity = 34/59 (57.63%), Postives = 47/59 (79.66%), Query Frame = -2 
Query: 2029 FNNIRFNIGKTISRAGVFSSMSYQSRFD-VREVLDNEL-KKGYKNLINNFVPIQRFFPR 2199
            F+ I+FNI K+ISRAG  SSM++QSRFD +++ LDNEL K+ Y++L N F  I+RFFP+
Sbjct:  767 FSKIKFNIRKSISRAGGLSSMNFQSRFDFLKDALDNELVKEDYRDLTNYFWHIRRFFPK 825
The following BLAST results are available for this feature:
BLAST of SMED30011848 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011848 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011848 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011848 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011848 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011848 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011848 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011848 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011848 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011848 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011848 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011848 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011848 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011848 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000081465.10.000e+099.65SMESG000081465.1[more]
SMESG000081465.10.000e+099.65SMESG000081465.1[more]
SMESG000032032.12.600e-1878.00SMESG000032032.1[more]
SMESG000069530.13.076e-1660.87SMESG000069530.1[more]
SMESG000041908.18.425e-1057.63SMESG000041908.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011848 ID=SMED30011848|Name=SMED30011848|organism=Schmidtea mediterranea sexual|type=transcript|length=3679bp
TCGGCATTCAATTATGAATAGTAGATTTGGTATTTTTCATTAAATTAAAA
ATACAAACGAATATTTCCAATGATTAAATTTATTCTATCTGACAATGAAC
AATATTTTCATAAATTTAACTGTGATATATCAATAAAAGAAAACCTTAAT
GATTTTTGTAAAGAAAAAGGATTAGTACCCGACATTTATGAAGTTTATGA
TCCGATTGAAGACAACGCATTAGATTGCAATAAAAGATTGTTGGATTATA
ATCTAAATTCTTTACATTTAAGACGAGTAAATTCACAATTCAGTAGCGAA
AAAAGTAGTCAAGACTTACTGAATTTTACTGACACTTCTGATAAACTATG
GAATGATCAATCTATCCGCACGTATTCCCAAAATTCCTACAATCAAATAC
AAATGTTAGATAATAACGCTCATAAGAAAATCAAAAAAGGCTTTATGTAT
CAATTGAAAAAATATATGCCAAAAAAAGATAAAAATATGTCAAGATCTTT
TTCAGAAAGTTTAGAATCTCCAGTAGAAACCGAAATGATAACTTTATCAA
CCGATGAAATCTACCAGGGATCATTAACAACAGAAATAATTCAAAGAGAT
ATTCAAAAAATTCAGAAAAACAATAAAAAAAAGAAGCGAAATGCCCCTGC
ACCTCCTAAGACAAATGTTCTCACTTTTTATTTCGAGGAAAATTCTGATT
CAAAACTGCAATCAATAGATATAGAAATTCCTTTACCTCCAGTTGATTCC
TTCGAACTTGGTGAACATATTAATTATTATCCCTGGCAATCATGCACCGA
ATGTTATACAGGTCAATGTTATGAAGATGAATCCGTCTATTCAATAATAT
CAGAAGATCCATTACCATTGTCACCCGGATTTCGTGTTTTTGAAACTCCT
AGGTGTCCCACACCTAAAGAGATGAGTGATGATGGAAGAGAAAGTCCTGA
CCACACTTATTCTACGATTGTTAAGAATAATAAATTTGATAAAAACATTG
ACAAAAGTGATATATCAGCCTCATCTAGCTTTAATGAAAAAGACCAATAT
TCAACTATCGCTAAAATACAAGATAAAAAAATATTTTATGGTGAAAATGT
GGAGCTGTCTAATAATCGCAGTTCGCAAGAATCCTTCCACGTTCAAGAAT
TATCAATATCGGACAATATTACAATTTTCAGTGCTCCAATTATATCTATA
GATTCTGATGGTTCATCAATTTCCTCTTTCAATAATGACTCAGTTTCTGA
AAAACAAAAAGATTGTGAATCTCAACATCATAAAGAAAACTGTATCTTAA
CATTTTCGCCGATTAAGTATAAAACAAATATTGACGAAGATATTTTAATT
AACAATAAACAATATTCTACAAATTTCAATTTCAAAACAGAAGAAACCAA
TTTCCCAAGCAATGTGGAACATAAAAATAACAACGATCTTATTTTACCAT
CCATAGAAATTTCCAGTTCTTCGGAACAAAACATTTCTATACAATCAAAT
ACTCTGCAAAATAATATATCAAATGAACAAAATAACAGCAGAAACGCAAA
CACTTTTCGGAATTTAGATGAAACCGACTCGAAAGAATTTCTGAAATCAA
ATCCACCTCAAGTTTTTTCCAAACCAAGGGTTAAAATAAATAACGCAGAG
AAAAAAGATACTCAAACAGCAAAATTGAATCATAAACCTAAAAATTCTCT
TATTCAACCAATTTATGACGATGGAAAAATGAGAATTGAAAAACAAGGAC
AAAGTTTATTTTTTTCTGTCAACATTGTTTTGGCGCTCATTCTATTTCAC
ATGGACACTGATACAACAAACCGTGATGATATAAAGTGAGATGATAAGGA
AGTGAGATCATTATTCAAGCTCTTTAATATGGCTTTTGTTCAACCATTAT
ATTAATAATAATGCTTATGAATATTCAGGAAATGATTTATTTTTTGAAAT
TAATTTAAAATAAAGTGAGTTAATTTTTTTAAGTAATTGAAAAAATTAAT
CTCCCTAAATATGTATTATTTTTATACATCTTGGAAAAAATCTCTGAATA
GGTACAAAATTATTAATCAAATTCTTATAACCTTTCTTCAGCTCGTTATC
TAGTACTTCCCGCACATCGAATCTGGATTGATATGACATGGAACTGAAAA
CACCTGCCCTTGAGATGGTCTTCCCAATATTGAATCTGATATTGTTGAAA
CTATGGAAATAATAAATTTATTATAAATAATGAATGAATCTTACAATATC
TTTTCTGCATCTTCAACAGAAATGTCTGAAATACTTATTAAAATAAATCC
TACGATTATATATTCTAATTTACACAAAAATTCCTAGTAAAATCAACAAA
ATTTACAAATGCATTATTAAAAATGACACACATTTTTTAATTCAAACCTC
TACAAACAATCAATGAAGCTCTTCAATCATTTGTCAATGATTTCTCAATC
AGTGAAATTACTGTTTTGGTTAATTCTTGTTTCCACTTATTCAAGTCATT
TCGAAACTCAACAAACTCATTTCCAAATACAAACTGTTGGAACTTATTCA
ACTATCAAATTATTGGTTTCTTTTTCTAACTTTTCTAACATCGTGATTTC
TCAAATCGTTTCTATTAACAGCACTGTGATACTCGAAGAAACGATAAATC
TTCATATCTCATGCTTATGGCTATTATCAACTGGCCAACAATCGAATGCA
AAGTTCAACTGATCAAATCAACATATTGGATTTATTTTGACTACATTTTT
GCTGTTTTTATTGTGTCTTCACAATATATTATAGAGAAGTTAGCGCAGCT
TTTTGTATCATCTGATGCCATATTAAGTCAAATAATGCCATATTTATCCA
GAGTATTTATTAAATTCGAAAAACCTGACTTTTCTCGTGATATATATTAA
CAAAATAAGAACAAGCAATTTATTTTCATGTAAGTTTAATGTTTAATAAT
AAACCATTTATTTCAAAATTACACCTTCAAAATTTTAAAATAACAAAACT
TATTTAATTTTAAATTTCACTTTTTTAAAATACTTATTCTAATTAAATCA
GTGGTCGGCAAACATTTTTGGTCGTGGTAAAATTCGTCTACGTGCTTCTT
CAATATTAAATACATGCTTCTTGATGTTTTCCAATTTTTCATCATTTGAC
ATCTCACTTACAGCTTTATGTTTGGACGATAATTGCTTCTCAATATTATA
GGTTCACCAGTAATTATATCCCCACATAAGCAACACACCACACGGTTACC
GATATATCCATATAATCTCACTTTTTTGACTTTGGTTCGGTCATGGTTAA
AAATCACAAAAAATACTTATATTCACCACAATATAATTATTATAATATAT
TTATTTACCAAATAAGTTCAATAAAAATGATTATTGGAACAATACTTCGT
ACGCCTACTTCAACTTTGCGTTTGAAATGAAAATTTAAAATTAAACATTA
AAACATAACTTGATATTTATTGTTTTGGAAACTTCTACAATTTTGAAGCG
GGTAACATGAAATGATGCTTTGGGTCACTCCGTGACCCACGGGTCACAGT
TTGCCGAGTGCTGATTTAAATATTTCTTTATAAAATCCAATTTATTATTT
AGCTTGATTATTTAGTTTTCATTTTACTGCTTGCTGATAGTTATGTCCAA
ATTCCCTGACATTTATCATATCCTTCCAC
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protein sequence of SMED30011848-orf-1

>SMED30011848-orf-1 ID=SMED30011848-orf-1|Name=SMED30011848-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=100bp
MKLFNHLSMISQSVKLLFWLILVSTYSSHFETQQTHFQIQTVGTYSTIKL
LVSFSNFSNIVISQIVSINSTVILEETINLHISCLWLLSTGQQSNAKFN*
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protein sequence of SMED30011848-orf-2

>SMED30011848-orf-2 ID=SMED30011848-orf-2|Name=SMED30011848-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=590bp
MIKFILSDNEQYFHKFNCDISIKENLNDFCKEKGLVPDIYEVYDPIEDNA
LDCNKRLLDYNLNSLHLRRVNSQFSSEKSSQDLLNFTDTSDKLWNDQSIR
TYSQNSYNQIQMLDNNAHKKIKKGFMYQLKKYMPKKDKNMSRSFSESLES
PVETEMITLSTDEIYQGSLTTEIIQRDIQKIQKNNKKKKRNAPAPPKTNV
LTFYFEENSDSKLQSIDIEIPLPPVDSFELGEHINYYPWQSCTECYTGQC
YEDESVYSIISEDPLPLSPGFRVFETPRCPTPKEMSDDGRESPDHTYSTI
VKNNKFDKNIDKSDISASSSFNEKDQYSTIAKIQDKKIFYGENVELSNNR
SSQESFHVQELSISDNITIFSAPIISIDSDGSSISSFNNDSVSEKQKDCE
SQHHKENCILTFSPIKYKTNIDEDILINNKQYSTNFNFKTEETNFPSNVE
HKNNNDLILPSIEISSSSEQNISIQSNTLQNNISNEQNNSRNANTFRNLD
ETDSKEFLKSNPPQVFSKPRVKINNAEKKDTQTAKLNHKPKNSLIQPIYD
DGKMRIEKQGQSLFFSVNIVLALILFHMDTDTTNRDDIK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell