SMED30011840

Overview
NameSMED30011840
Smed IDSMED30011840
Length (bp)1441
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30011840 (SMED30011840) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30011840 (SMED30011840) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30011840 (SMED30011840) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30011840 (SMED30011840) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30011840

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 18

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30011840h1SMcG0021709 Contig2280newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
Smed sexual biotypeSMED30011840h1SMcG0002848 Contig4116newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30011840h1SMcG0002848 Contig4116uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30011840h1SMcG0021709 dd_Smed_v4_3516_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30011840h1SMcG0021709 dd_Smed_v4_3516_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30011840h1SMcG0021709 dd_Smed_v4_3516_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30011840h1SMcG0021709 dd_Smed_v4_3516_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30011840h1SMcG0021709 dd_Smed_v4_3516_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30011840h1SMcG0021709 dd_Smed_v4_3516_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30011840h1SMcG0021709 dd_Smed_v4_3516_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30011840h1SMcG0021709 dd_Smed_v4_3516_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30011840h1SMcG0021709 dd_Smed_v4_3516_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30011840h1SMcG0021709 Contig4116newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30011840h1SMcG0021709 Contig4116uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
muscle cellSMED30011840h1SMcG0021709 dd_Smed_v4_14517_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30011840h1SMcG0021709 dd_Smed_v6_3516_1_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Category 3 cellSMED30011840h1SMcG0021709 dd_Smed_v4_3516_1_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30011840h1SMcG0021709 dd_Smed_v4_3516_1_1dd_Smed_v4PMID:30962434
Cote et al., 2019		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30011840 vs. TrEMBL
Match: A0A419PS41 (Uncharacterized protein (Fragment) OS=Clonorchis sinensis OX=79923 GN=CSKR_12385s PE=4 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 4.230e-20
Identity = 66/238 (27.73%), Postives = 123/238 (51.68%), Query Frame = -1 
Query:  329 WNLLTMIEEKYKIGLSATDSDFLLNLTSKEHG--FQFPAQTIFPKLKIVSNAF-IYIKFFP----ISTAFTILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFKNYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVNQQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEMLEGS--GSQTKKWKPKYIGNYVI 1015
            W++  ++  +Y+I  +  D DF + +     G  +  PA  +FP  ++ ++ F I++ F P    +     +  IRDP N +RL +    Q+ H   +++V+ Y+P+ ++E   +  ++ ++P+ ++ W LG+  E  +   YTSC D  +   YH V  T  +  +LF  G T + LN+G + G+ E + G +  F   +  +  + MC K E+ EGS  GS T  + P + GN ++
Sbjct:   31 WDITYLLNRRYEI--ARADEDFPIVIQPPADGTLYHVPAMRLFPDAQLPNDTFFIHMLFKPQKELLERGVYLFSIRDPDNTLRLSL----QVVHRSAYQVVLTYNPVESVEG--EKSVDFSVPWTDAYWHLGIHFEQNQTKFYTSCSDT-ETVPYHTVPTTGILGTRLFRDGATFFALNSG-LQGSPEYYVGMLKSFNFASTTDAVKQMCTKSEVPEGSGEGSLTIDFDPTF-GNILV 257
[Close]
BLAST of SMED30011840 vs. TrEMBL
Match: A0A4E0RP53 (Collagen alpha 1(XV) chain OS=Fasciola hepatica OX=6192 GN=D915_000354 PE=4 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 4.531e-20
Identity = 69/245 (28.16%), Postives = 120/245 (48.98%), Query Frame = -1 
Query:  368 LLYFSIIFGIVQNSVTIEHRKEWNLLTMIEEKYKIGLSATDSDFLLNLTSKEHG--FQFPAQTIFPKLKIVSNAFIYIKFF-----PISTAFTILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFKNYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVNQQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEMLEGSGSQT 1081
             L+F I+   +   V+      W++  ++  +Y I  +  D DF + +   E G  +  PA  +FP+ K+  + F     F     P+     ++ IRDP   +RL +R    L H   ++++  Y P+ + E +    +E +IP+ +  W LG+LIE ++I   TSC D  +   Y+ V+ T  +  +LF  G T Y LN+G ++ + + F G +  F   +  +  + MCRK E  +GSG  T
Sbjct:   16 FLHFWILLSTLA-IVSAAQDVNWDITFLLNRRYDI--ARADEDFPILIQPPEDGTLYHVPAMRLFPEGKLPPDTFFIHLMFKPRKEPLDRGAYMMAIRDPDGTLRLSLR----LVHVSTYQVIFTYHPVESEEEK---QIEFSIPWTDEYWHLGILIEKEQIKFLTSCQDSLE-VPYNTVNATNLLGTRLFRDGATFYALNSG-LHRSPDYFSGLLKSFYFHSSTDAAKDMCRKSEPSDGSGDGT 248
[Close]
BLAST of SMED30011840 vs. TrEMBL
Match: A0A3Q0KKZ7 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 3.094e-19
Identity = 80/264 (30.30%), Postives = 132/264 (50.00%), Query Frame = -1 
Query:  329 QLNLVLILLYFSIIFGIVQNSVTIEHRKEWNLLTMIEEKYKIGLSATDSDFLLNLTSKEHGFQFPAQTIFPKLKIVSNAF-IYIKFFPISTAFT----ILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFKNYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVNQQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEMLEGSGSQTK-KWKPKYIGNYVI 1102
            +LN +LIL +      I+  +V  E    W++  ++  +Y I  +  D    +   S +  +  PA  +FP+ K+    F I++ F P  TA      +  IRDP N +RL ++    +  +  ++++  Y+P+S   N     +E +IP+ +  W LG+LI+  KI  YTSC++  +   YH  S    + + LF  G T Y LN+G  N + E FEG +  F   +  N  +YMC K E   GSG   + K+ P++ GN +I
Sbjct:   15 KLNYILILTF---TVSIILPTVFSEQGVNWDITYLLNRRYDIARADEDFPIKIQPPSGDVLYSVPALRLFPQSKLPDETFFIHLIFKPNKTAIQHGLYLFSIRDPDNVLRLGLK----IIEYTTYQIIFAYNPLSFDGN--SKEVEFSIPWNDDYWHLGILIKPDKIYFYTSCEET-QGIAYHFYSIQGLLGEPLFRKGATFYALNSGLKNAS-EYFEGYLKAFSFHSDENALQYMCTKSENYGGSGDADRFKYDPEF-GNILI 266
[Close]
BLAST of SMED30011840 vs. TrEMBL
Match: A0A074ZKP1 (Uncharacterized protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=T265_14024 PE=4 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 2.733e-18
Identity = 59/220 (26.82%), Postives = 111/220 (50.45%), Query Frame = -1 
Query:  377 WNLLTMIEEKYKIGLSATDSDFLLNLTSKEHG--FQFPAQTIFPKLKIVSNAF-IYIKFFP----ISTAFTILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFKNYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVNQQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEMLEGSG 1015
            W++  ++  +Y+I  +  D DF + +     G  +  PA  +FP+ ++ ++ F I++ F P    +     +  IRDP N +R  +    Q+ H   +++V+ Y+P+ ++E   +  ++ ++P+ ++ W LG+  E  +   YTSC D  +   YH V  T  +  +LF  G T + LN+G +    E + G +  F   +  +    MC K E+ EGSG
Sbjct:    4 WDITYLLNRRYEI--ARADEDFPIVIQPPADGTLYHVPAMRLFPEAQLPNDTFFIHMLFKPQKESLEHGVYLFSIRDPDNTLRFSL----QVVHRSAYQVVLTYNPVESVEG--EKSVDFSVPWTDAYWHLGIQFEQNQTKFYTSCSDT-ETVPYHTVPTTGILGTRLFRDGATFFALNSG-LQSAPEYYVGMLKSFNFASTTDAVRQMCTKSEIPEGSG 213
[Close]
BLAST of SMED30011840 vs. TrEMBL
Match: A0A5K3FPE2 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.852e-18
Identity = 75/271 (27.68%), Postives = 133/271 (49.08%), Query Frame = -1 
Query:  329 IHFIIMNQLNLVLILL-YFSIIFGIVQNSVTIEHRKEWNLLTMIEEKYKIGLSATDSDFLLNLTSKEHG--FQFPAQTIFPKLKIVSNA-FIYIKFFPIS--TAFTILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFKNYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVNQQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEMLEGSGSQTKKWKPKYIGNYVI 1123
            I F  M  +N +LI+L  FS +  +V  +     R  W++  ++  KY +    +D DF +++++ E G  +  PAQTIF   K      +I + F P +  T F  L IRDP + +R  +  Y++  +  Q R  ++ S      N  +  ++   P   S W LGVLI+   +  +T+C   F+   +H+ +    + + LF  G + YLLN+G  +  K   EG ++EF   + +++    C+   + EGSG     + P++ GN ++
Sbjct:    8 ISFTSMRLINRILIVLTVFSALVTLVGGTT----RPNWDVSFLLNRKYGLTAMNSDLDFPMDISTPEDGLLYSVPAQTIFSDKKFPDEPIYIQLNFQPNTSLTEFYFLTIRDPDDVMRFSLSMYKRNMYRVQMRYNLLES------NEGERTIDFPYPQPPSYWRLGVLIKENSVQFFTNCPR-FQTAPFHEETGVKLLGRPLFRDGASAYLLNSGRQSSPK-YLEGVLTEFTFHSGHDVEMAPCKTTVLEEGSGEPMYTFDPEF-GNVIV 265
[Close]
BLAST of SMED30011840 vs. Planmine SMEST
Match: SMESG000081921.1 (SMESG000081921.1)

HSP 1 Score: 551.592 bits (1420), Expect = 0.000e+0
Identity = 260/260 (100.00%), Postives = 260/260 (100.00%), Query Frame = -1 
Query:  329 MNQLNLVLILLYFSIIFGIVQNSVTIEHRKEWNLLTMIEEKYKIGLSATDSDFLLNLTSKEHGFQFPAQTIFPKLKIVSNAFIYIKFFPISTAFTILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFKNYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVNQQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEMLEGSGSQTKKWKPKYIGNYVI 1108
            MNQLNLVLILLYFSIIFGIVQNSVTIEHRKEWNLLTMIEEKYKIGLSATDSDFLLNLTSKEHGFQFPAQTIFPKLKIVSNAFIYIKFFPISTAFTILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFKNYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVNQQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEMLEGSGSQTKKWKPKYIGNYVI
Sbjct:    1 MNQLNLVLILLYFSIIFGIVQNSVTIEHRKEWNLLTMIEEKYKIGLSATDSDFLLNLTSKEHGFQFPAQTIFPKLKIVSNAFIYIKFFPISTAFTILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFKNYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVNQQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEMLEGSGSQTKKWKPKYIGNYVI 260
[Close]
BLAST of SMED30011840 vs. Planmine SMEST
Match: SMESG000081921.1 (SMESG000081921.1)

HSP 1 Score: 550.436 bits (1417), Expect = 0.000e+0
Identity = 260/260 (100.00%), Postives = 260/260 (100.00%), Query Frame = -1 
Query:  329 MNQLNLVLILLYFSIIFGIVQNSVTIEHRKEWNLLTMIEEKYKIGLSATDSDFLLNLTSKEHGFQFPAQTIFPKLKIVSNAFIYIKFFPISTAFTILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFKNYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVNQQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEMLEGSGSQTKKWKPKYIGNYVI 1108
            MNQLNLVLILLYFSIIFGIVQNSVTIEHRKEWNLLTMIEEKYKIGLSATDSDFLLNLTSKEHGFQFPAQTIFPKLKIVSNAFIYIKFFPISTAFTILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFKNYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVNQQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEMLEGSGSQTKKWKPKYIGNYVI
Sbjct:    1 MNQLNLVLILLYFSIIFGIVQNSVTIEHRKEWNLLTMIEEKYKIGLSATDSDFLLNLTSKEHGFQFPAQTIFPKLKIVSNAFIYIKFFPISTAFTILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFKNYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVNQQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEMLEGSGSQTKKWKPKYIGNYVI 260
[Close]
BLAST of SMED30011840 vs. Planmine SMEST
Match: SMESG000081921.1 (SMESG000081921.1)

HSP 1 Score: 548.895 bits (1413), Expect = 0.000e+0
Identity = 260/260 (100.00%), Postives = 260/260 (100.00%), Query Frame = -1 
Query:  329 MNQLNLVLILLYFSIIFGIVQNSVTIEHRKEWNLLTMIEEKYKIGLSATDSDFLLNLTSKEHGFQFPAQTIFPKLKIVSNAFIYIKFFPISTAFTILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFKNYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVNQQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEMLEGSGSQTKKWKPKYIGNYVI 1108
            MNQLNLVLILLYFSIIFGIVQNSVTIEHRKEWNLLTMIEEKYKIGLSATDSDFLLNLTSKEHGFQFPAQTIFPKLKIVSNAFIYIKFFPISTAFTILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFKNYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVNQQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEMLEGSGSQTKKWKPKYIGNYVI
Sbjct:    1 MNQLNLVLILLYFSIIFGIVQNSVTIEHRKEWNLLTMIEEKYKIGLSATDSDFLLNLTSKEHGFQFPAQTIFPKLKIVSNAFIYIKFFPISTAFTILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFKNYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVNQQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEMLEGSGSQTKKWKPKYIGNYVI 260
[Close]
BLAST of SMED30011840 vs. Planmine SMEST
Match: SMESG000081921.1 (SMESG000081921.1)

HSP 1 Score: 483.026 bits (1242), Expect = 1.493e-163
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = -1 
Query:  329 MIEEKYKIGLSATDSDFLLNLTSKEHGFQFPAQTIFPKLKIVSNAFIYIKFFPISTAFTILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFKNYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVNQQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEMLEGSGSQTKKWKPKYIGNYVI 1000
            MIEEKYKIGLSATDSDFLLNLTSKEHGFQFPAQTIFPKLKIVSNAFIYIKFFPISTAFTILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFKNYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVNQQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEMLEGSGSQTKKWKPKYIGNYVI
Sbjct:    1 MIEEKYKIGLSATDSDFLLNLTSKEHGFQFPAQTIFPKLKIVSNAFIYIKFFPISTAFTILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFKNYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVNQQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEMLEGSGSQTKKWKPKYIGNYVI 224
[Close]
BLAST of SMED30011840 vs. Planmine SMEST
Match: SMESG000081921.1 (SMESG000081921.1)

HSP 1 Score: 476.863 bits (1226), Expect = 4.856e-160
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = -1 
Query:  329 MIEEKYKIGLSATDSDFLLNLTSKEHGFQFPAQTIFPKLKIVSNAFIYIKFFPISTAFTILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFKNYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVNQQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEMLEGSGSQTKKWKPKYIGNYVI 1000
            MIEEKYKIGLSATDSDFLLNLTSKEHGFQFPAQTIFPKLKIVSNAFIYIKFFPISTAFTILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFKNYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVNQQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEMLEGSGSQTKKWKPKYIGNYVI
Sbjct:    1 MIEEKYKIGLSATDSDFLLNLTSKEHGFQFPAQTIFPKLKIVSNAFIYIKFFPISTAFTILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFKNYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVNQQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEMLEGSGSQTKKWKPKYIGNYVI 224
The following BLAST results are available for this feature:
BLAST of SMED30011840 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011840 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011840 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011840 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011840 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011840 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011840 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011840 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A419PS414.230e-2027.73Uncharacterized protein (Fragment) OS=Clonorchis s... [more]
A0A4E0RP534.531e-2028.16Collagen alpha 1(XV) chain OS=Fasciola hepatica OX... [more]
A0A3Q0KKZ73.094e-1930.30Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A074ZKP12.733e-1826.82Uncharacterized protein (Fragment) OS=Opisthorchis... [more]
A0A5K3FPE23.852e-1827.68Uncharacterized protein OS=Mesocestoides corti OX=... [more]
back to top
BLAST of SMED30011840 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011840 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011840 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011840 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011840 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011840 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000081921.10.000e+0100.00SMESG000081921.1[more]
SMESG000081921.10.000e+0100.00SMESG000081921.1[more]
SMESG000081921.10.000e+0100.00SMESG000081921.1[more]
SMESG000081921.11.493e-163100.00SMESG000081921.1[more]
SMESG000081921.14.856e-160100.00SMESG000081921.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011840 ID=SMED30011840|Name=SMED30011840|organism=Schmidtea mediterranea sexual|type=transcript|length=1441bp
GCTGCAAGGTTGTTGTAGATTCTTTAGTTGTTGTTGGCTGCAAAGTTGTT
GTAGATTCTGTAGTTGTAGTTAGTTTAGAGGTTGTCGAAAATTCTGTGGT
TGTTGTTGGTTGAGAGGTAGTAGTAGATTCCGTACTTGTTGTTGGCTGCA
AAATTGTTGTAGATTCTGTGGTTGTTTTTGGTTGATAAGTTGTGATGGAA
GTAGCAGAAGTTGTCTTTTGTTGAGTGGAAGTTGTTGATTGATAGTTTTT
GGTTGTAGTTAATGTTGAACCAGTAGTCGATGTGGTAAAAGTTGATGGAA
ATGATGTTGTTTCAATAGTACTTGAAGTGATGACATAATTTCCAATATAT
TTTGGTTTCCACTTCTTTGTCTGTGAACCACTTCCTTCTAGCATCTCTTC
TTTTCGACACATGTACTCATTCAAATTGTTTTTATTAGTTAACACAAACT
CCGAAACCGTGCCTTCAAATATTTCCTTATTTCCATTAATTTCTCCATTA
TTCAGCAAATATAATGTAGTTCCAGAGTAAAATAACTGCTGATTTACGGG
ATAAGTATTTGACACTTGATGGTAACCATATGGCTTGAATATATCATCAC
AGCTAGTGTACACCGTGATCTTTTTATTTTCAATCAATACACCAAGAGTC
CATGCTGAATTCAGAAATGGGATTGTTAATTCCATGTAATTTTTGAATCT
GTTCTCTATATTAGAAATAGGACTATATACGATGACGAGTCGAAACTGTT
TGAAATGTCCAAGTTGCTGATATCCACGTATATCTAATCTGACTTTGTTT
AATGGATCTCGTATTTTCAAAATTGTGAATGCAGTAGATATTGGAAAAAA
TTTGATATAGATAAAAGCATTAGACACAATCTTCAGTTTAGGAAAAATTG
TTTGAGCTGGAAATTGAAAGCCATGTTCCTTCGACGTAAGATTTAACAAA
AAGTCACTGTCTGTCGCACTAAGGCCGATTTTATATTTTTCTTCAATCAT
TGTCAATAAATTCCATTCTTTTCGATGTTCTATTGTTACGGAATTTTGAA
CTATTCCAAATATTATTGAAAAATATAAAAGAATTAATACCAAATTTAGC
TGATTCATTATAATAAAATGAATTTCAAACACTATTTTCATGATTCAATT
TATTCTACAACAAACCAATCATCATTGACAGATTAAACTGATCTAGATCA
TAGTAAATTACAAATTTTCTCTCCGGTTTTGTAAATAAGCCGGGCATATT
TTGCTACTCTTTCTTTATTAAATATTAAAAACACACTCCTGTGCAATTAA
TAATCAAAGAAACAAAATAAACTTAATTAACAAGTTTCGCATTCCTTTTA
TAAATTCTGAATAGTAATCGCTTGGATTATCATTTATATTTCATTTGATT
TGTAAGTAATTAACAAACTTTTAAAATAATCCATGTTATTT
back to top

protein sequence of SMED30011840-orf-1

>SMED30011840-orf-1 ID=SMED30011840-orf-1|Name=SMED30011840-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=380bp
MKIVFEIHFIIMNQLNLVLILLYFSIIFGIVQNSVTIEHRKEWNLLTMIE
EKYKIGLSATDSDFLLNLTSKEHGFQFPAQTIFPKLKIVSNAFIYIKFFP
ISTAFTILKIRDPLNKVRLDIRGYQQLGHFKQFRLVIVYSPISNIENRFK
NYMELTIPFLNSAWTLGVLIENKKITVYTSCDDIFKPYGYHQVSNTYPVN
QQLFYSGTTLYLLNNGEINGNKEIFEGTVSEFVLTNKNNLNEYMCRKEEM
LEGSGSQTKKWKPKYIGNYVITSSTIETTSFPSTFTTSTTGSTLTTTKNY
QSTTSTQQKTTSATSITTYQPKTTTESTTILQPTTSTESTTTSQPTTTTE
FSTTSKLTTTTESTTTLQPTTTKESTTTLQ
back to top
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 283..378
NoneNo IPR availableCDDcd16882ARID_ARID4Acoord: 113..174
e-value: 0.00204121
score: 34.9491
NoneNo IPR availableTMHMMTMhelixcoord: 9..31