SMED30011819
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30011819 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 11
Alignments
SMED30011819 aligns in the following genomic locations:
Homology
BLAST of SMED30011819 vs. Ensembl Human
Match: MIB1 (mindbomb E3 ubiquitin protein ligase 1 [Source:HGNC Symbol;Acc:HGNC:21086]) HSP 1 Score: 277.33 bits (708), Expect = 2.125e-78 Identity = 201/673 (29.87%), Postives = 339/673 (50.37%), Query Frame = -1
Query: 120 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXX-----------XXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCES--VGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFL-KREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMG---------ILGDMTEGSD--TPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQ-DLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2048
L+ GD V + D++ + LQ HGGW M G + I D+ V +G +NP + + + F GD V++ D ++KLLQ+ HGEW +M LGK G+V+ +++D DL+V++ + T NP V S + + + E LS+L+ + + D EE++K A NG++ V +L + V V+ + G + A GH+D++KLL+++ ++ A+DKDGD A+H A ++ + ++L AD+NA+N + TP+ +A + G +++ + L S+ DS+ DT LH A+ K R + + +LL+AGAD+ N FN H AAL+G+ + + L K IVD +K+D + A+HL ALNNH + +L+ A+++I+N QTALH+AV ++H V++LV A+++++D DGDTPLH A++ + +++ M +G + I+G T+G++ + IA +L +GA L ++NKKG +P+++C D PN K KE G R P I + E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 246 LQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVASAGSAISN--ASGERLSQLLKKLFETQESGDL------------NEELVK---AAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPD------------PNLCKALAKCHKEKVSGQVGSR------------SPSMISNDSETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 863 HSP 2 Score: 79.7221 bits (195), Expect = 1.707e-14 Identity = 53/156 (33.97%), Postives = 82/156 (52.56%), Query Frame = -1
Query: 2304 GLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDA--IVRGPSGNVYRLNSELLSK----YGSDTSQESISGY-------VVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIA 2732
GL+ GD V I + DLE + +Q HGGW D M E L V GI D +V+ PSGN + N +L+K D +Q + G +V++ D ++ + LQ GHGEW + M +GK +V + D D+K++ G+++ NP A++
Sbjct: 245 GLQIGDLVNIDL-DLEIVQSLQHGHGGWTDGMFETLTTT-GTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVS 398 HSP 3 Score: 66.2402 bits (160), Expect = 2.414e-10 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = -1
Query: 117 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 299
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 946 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY 1006 HSP 4 Score: 63.5438 bits (153), Expect = 2.009e-9 Identity = 41/174 (23.56%), Postives = 78/174 (44.83%), Query Frame = -1
Query: 2067 GNVYRLNSELLSKYGSDTSQESIS-GYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKY-NGSTFVINPKAIASKN------EKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2564
G+ YR + +L + + + + G +V + D + + LQ GHG W D M + + V D D+ ++Y +G+ + NP + N +F++G+ +++ +K LQ HG W++ ++ GK + +I+ D D+ V G W NP +
Sbjct: 224 GSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV 397
BLAST of SMED30011819 vs. Ensembl Human
Match: MIB2 (mindbomb E3 ubiquitin protein ligase 2 [Source:HGNC Symbol;Acc:HGNC:30577]) HSP 1 Score: 258.455 bits (659), Expect = 6.157e-72 Identity = 197/671 (29.36%), Postives = 319/671 (47.54%), Query Frame = -1
Query: 123 IDVKDNILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNG-LGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNG----ELNNVRNLCESVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNS--VEILLD-AGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLM--------GILG--DMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDD-LLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGH-VACDVCANNLYECHICRESIENKIR 2069
+ + GD V D +++Q+ HGGW RM+ F G+ G + I GDV+V N ++P LTK F GD VR+I D VK LQ HGEW M LG+ GKV +F DG+LRV + Q T +P C+ A + D+ D+ +++ D+ + + E +L V G L+ +R E V D + +G L +A G +++++LL++ RA ++ D +G+TALH A NQ + +L++ G +A N+ + T + +A G +E+ R L R D+++ D+ +DT LH A+ S VE+L + D+ A N + F H A+L+GHA + L R ++VD++KED F A+HL ALNNH + ++L+ + D+N+RN LQ+ LH+AV Q H+ V +LV+ +N D +GDT LH+A++ Q+ G G L G+ G ++T G+ +A +L GA + N +G +P+++ + +LKA Q E++ G AP P + L P + + L + C++C + + V F C H C+ CA + +C C+ + K+R
Sbjct: 264 VSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHS-----FWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKS-----------DPEHPGRLVVEVALGNAARALDLLRRRPEQV--DTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALC-ERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILAR-ARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAA----VACFLALEGADVSYTNHRGRSPLDLAAEGRVLKAL-------QGCAQRFRERQAGGGA------AP-GPRQTLGTPNTVTN-------LHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLR 889 HSP 2 Score: 95.9005 bits (237), Expect = 2.121e-19 Identity = 51/161 (31.68%), Postives = 87/161 (54.04%), Query Frame = -1
Query: 2067 SELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKYNGST-FVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2543
+EL + +D SQ G V+ + D R++Q GHG WN M + IG+ +H+ D GDV++++N T + +P A+ H F +G+ IG++DT+K+LQ HG W+D + G+ ++V++F DG++ V+ G W +P C+
Sbjct: 258 AELQRRVSAD-SQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQ-TGTVHRITDRGDVRVQFNHETRWTFHPGALTKH----------HSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCL 406 HSP 3 Score: 69.707 bits (169), Expect = 2.138e-11 Identity = 50/176 (28.41%), Postives = 77/176 (43.75%), Query Frame = -1
Query: 2196 DVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKY--GSDTSQESI-SGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQEN 2714
D V + D + + +MQ HGGWN M E IG V I VR + N E + G+ T S G VV +I D ++LQ GHGEW D+M +G+ +V+ F DG++++ G + +P + + R E N+D ++ +EN
Sbjct: 275 DKVKCLLDTDVLREMQEGHGGWNPRMAE-FIGQTGTVHRITDRGDVR------VQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCL-----------------VAYRPEEDANLDVAERAREN 426
BLAST of SMED30011819 vs. Ensembl Human
Match: MIB2 (mindbomb E3 ubiquitin protein ligase 2 [Source:HGNC Symbol;Acc:HGNC:30577]) HSP 1 Score: 258.84 bits (660), Expect = 1.327e-71 Identity = 195/670 (29.10%), Postives = 317/670 (47.31%), Query Frame = -1
Query: 123 IDVKDNILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNG-LGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNG----ELNNVRNLCESVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNS--VEILLD-AGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLM--------GILG--DMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGH-VACDVCANNLYECHICRESIENKIR 2069
+ + GD V D +++Q+ HGGW RM+ F G+ G + I GDV+V N ++P LTK F GD VR+I D VK LQ HGEW M LG+ GKV +F DG+LRV + Q T +P C+ A + D+ D+ +++ D+ + + E +L V G L+ +R E V D + +G L +A G +++++LL++ RA ++ D +G+TALH A NQ + +L++ G +A N+ + T + +A G +E+ R L R D+++ D+ +DT LH A+ S VE+L + D+ A N + F H A+L+GHA + L R ++VD++KED F A+HL ALNNH + ++L+ + D+N+RN LQ+ LH+AV Q H+ V +LV+ +N D +GDT LH+A++ Q+ G G L G+ G ++T G+ +A +L GA + N +G +P+++ + + L Q E++ G AP P + L P + + L + C++C + + V F C H C+ CA + +C C+ + K+R
Sbjct: 365 VSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHS-----FWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKS-----------DPEHPGRLVVEVALGNAARALDLLRRRPEQV--DTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALC-ERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILAR-ARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAA----VACFLALEGADVSYTNHRGRSPLDLAAEGRV------LKALQGCAQRFRERQAGGGA------AP-GPRQTLGTPNTVTN-------LHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLR 990 HSP 2 Score: 96.2857 bits (238), Expect = 1.403e-19 Identity = 51/161 (31.68%), Postives = 87/161 (54.04%), Query Frame = -1
Query: 2067 SELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKYNGST-FVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2543
+EL + +D SQ G V+ + D R++Q GHG WN M + IG+ +H+ D GDV++++N T + +P A+ H F +G+ IG++DT+K+LQ HG W+D + G+ ++V++F DG++ V+ G W +P C+
Sbjct: 359 AELQRRVSAD-SQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQ-TGTVHRITDRGDVRVQFNHETRWTFHPGALTKH----------HSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCL 507 HSP 3 Score: 70.0922 bits (170), Expect = 1.595e-11 Identity = 57/210 (27.14%), Postives = 89/210 (42.38%), Query Frame = -1
Query: 2196 FFY*DCKIKIKFLHKEI*IILYMGASLSGLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKY--GSDTSQESI-SGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQEN 2816
F+Y D + L K + + A + GD V + D + + +MQ HGGWN M E IG V I VR + N E + G+ T S G VV +I D ++LQ GHGEW D+M +G+ +V+ F DG++++ G + +P + + R E N+D ++ +EN
Sbjct: 346 FYYKD---HLPRLGKPAELQRRVSADSQPFQHGDKVKCLL-DTDVLREMQEGHGGWNPRMAE-FIGQTGTVHRITDRGDVR------VQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCL-----------------VAYRPEEDANLDVAERAREN 527
BLAST of SMED30011819 vs. Ensembl Human
Match: MIB2 (mindbomb E3 ubiquitin protein ligase 2 [Source:HGNC Symbol;Acc:HGNC:30577]) HSP 1 Score: 257.684 bits (657), Expect = 1.439e-71 Identity = 195/670 (29.10%), Postives = 317/670 (47.31%), Query Frame = -1
Query: 123 IDVKDNILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNG-LGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNG----ELNNVRNLCESVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNS--VEILLD-AGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLM--------GILG--DMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGH-VACDVCANNLYECHICRESIENKIR 2069
+ + GD V D +++Q+ HGGW RM+ F G+ G + I GDV+V N ++P LTK F GD VR+I D VK LQ HGEW M LG+ GKV +F DG+LRV + Q T +P C+ A + D+ D+ +++ D+ + + E +L V G L+ +R E V D + +G L +A G +++++LL++ RA ++ D +G+TALH A NQ + +L++ G +A N+ + T + +A G +E+ R L R D+++ D+ +DT LH A+ S VE+L + D+ A N + F H A+L+GHA + L R ++VD++KED F A+HL ALNNH + ++L+ + D+N+RN LQ+ LH+AV Q H+ V +LV+ +N D +GDT LH+A++ Q+ G G L G+ G ++T G+ +A +L GA + N +G +P+++ + + L Q E++ G AP P + L P + + L + C++C + + V F C H C+ CA + +C C+ + K+R
Sbjct: 300 VSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHS-----FWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKS-----------DPEHPGRLVVEVALGNAARALDLLRRRPEQV--DTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALC-ERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILAR-ARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAA----VACFLALEGADVSYTNHRGRSPLDLAAEGRV------LKALQGCAQRFRERQAGGGA------AP-GPRQTLGTPNTVTN-------LHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLR 925 HSP 2 Score: 98.9821 bits (245), Expect = 2.018e-20 Identity = 56/177 (31.64%), Postives = 94/177 (53.11%), Query Frame = -1
Query: 2067 IVRGPS---GNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKYNGST-FVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2582
+VRGP G+ +EL + +D SQ G V+ + D R++Q GHG WN M + IG+ +H+ D GDV++++N T + +P A+ H F +G+ IG++DT+K+LQ HG W+D + G+ ++V++F DG++ V+ G W +P C+
Sbjct: 278 VVRGPDWEWGSQDGKPAELQRRVSAD-SQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQ-TGTVHRITDRGDVRVQFNHETRWTFHPGALTKH----------HSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCL 442 HSP 3 Score: 70.4774 bits (171), Expect = 1.467e-11 Identity = 50/176 (28.41%), Postives = 77/176 (43.75%), Query Frame = -1
Query: 2196 DVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKY--GSDTSQESI-SGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQEN 2714
D V + D + + +MQ HGGWN M E IG V I VR + N E + G+ T S G VV +I D ++LQ GHGEW D+M +G+ +V+ F DG++++ G + +P + + R E N+D ++ +EN
Sbjct: 311 DKVKCLLDTDVLREMQEGHGGWNPRMAE-FIGQTGTVHRITDRGDVR------VQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCL-----------------VAYRPEEDANLDVAERAREN 462
BLAST of SMED30011819 vs. Ensembl Human
Match: MIB2 (mindbomb E3 ubiquitin protein ligase 2 [Source:HGNC Symbol;Acc:HGNC:30577]) HSP 1 Score: 258.455 bits (659), Expect = 1.505e-71 Identity = 195/670 (29.10%), Postives = 317/670 (47.31%), Query Frame = -1
Query: 123 IDVKDNILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNG-LGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNG----ELNNVRNLCESVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNS--VEILLD-AGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLM--------GILG--DMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGH-VACDVCANNLYECHICRESIENKIR 2069
+ + GD V D +++Q+ HGGW RM+ F G+ G + I GDV+V N ++P LTK F GD VR+I D VK LQ HGEW M LG+ GKV +F DG+LRV + Q T +P C+ A + D+ D+ +++ D+ + + E +L V G L+ +R E V D + +G L +A G +++++LL++ RA ++ D +G+TALH A NQ + +L++ G +A N+ + T + +A G +E+ R L R D+++ D+ +DT LH A+ S VE+L + D+ A N + F H A+L+GHA + L R ++VD++KED F A+HL ALNNH + ++L+ + D+N+RN LQ+ LH+AV Q H+ V +LV+ +N D +GDT LH+A++ Q+ G G L G+ G ++T G+ +A +L GA + N +G +P+++ + + L Q E++ G AP P + L P + + L + C++C + + V F C H C+ CA + +C C+ + K+R
Sbjct: 351 VSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHS-----FWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKS-----------DPEHPGRLVVEVALGNAARALDLLRRRPEQV--DTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALC-ERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILAR-ARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAA----VACFLALEGADVSYTNHRGRSPLDLAAEGRV------LKALQGCAQRFRERQAGGGA------AP-GPRQTLGTPNTVTN-------LHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLR 976 HSP 2 Score: 95.9005 bits (237), Expect = 1.783e-19 Identity = 51/161 (31.68%), Postives = 87/161 (54.04%), Query Frame = -1
Query: 2067 SELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKYNGST-FVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2543
+EL + +D SQ G V+ + D R++Q GHG WN M + IG+ +H+ D GDV++++N T + +P A+ H F +G+ IG++DT+K+LQ HG W+D + G+ ++V++F DG++ V+ G W +P C+
Sbjct: 345 AELQRRVSAD-SQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQ-TGTVHRITDRGDVRVQFNHETRWTFHPGALTKH----------HSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCL 493 HSP 3 Score: 69.707 bits (169), Expect = 2.014e-11 Identity = 57/210 (27.14%), Postives = 89/210 (42.38%), Query Frame = -1
Query: 2196 FFY*DCKIKIKFLHKEI*IILYMGASLSGLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKY--GSDTSQESI-SGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQEN 2816
F+Y D + L K + + A + GD V + D + + +MQ HGGWN M E IG V I VR + N E + G+ T S G VV +I D ++LQ GHGEW D+M +G+ +V+ F DG++++ G + +P + + R E N+D ++ +EN
Sbjct: 332 FYYKD---HLPRLGKPAELQRRVSADSQPFQHGDKVKCLL-DTDVLREMQEGHGGWNPRMAE-FIGQTGTVHRITDRGDVR------VQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCL-----------------VAYRPEEDANLDVAERAREN 513
BLAST of SMED30011819 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 127.487 bits (319), Expect = 2.016e-29 Identity = 90/308 (29.22%), Postives = 154/308 (50.00%), Query Frame = -1
Query: 471 GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMK---VKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMC 1385
G PL +A+ +GH VV +L+E ++K K ALH+A K+ LL+ + + + + TP+ +AA G + +LL + A+++ N + LH+A + R N +LL GA I++ K L H AA GH ++++L + +G I ++ ++ A +H+ A +H D R LL ++A ++ T LH+A H+ K+L++ +A+ N R +G TPLHIA K +K +E K + G+ +I IYLL+ GA D++ +G TP+ +
Sbjct: 165 GFTPLAVALQQGHDRVVAVLLEN----DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLL-LEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVV-QGAPI-SAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLA 465 HSP 2 Score: 123.635 bits (309), Expect = 2.924e-28 Identity = 95/343 (27.70%), Postives = 163/343 (47.52%), Query Frame = -1
Query: 471 LFFAVKNGELNNVRNLCES-VGVDARLKGIY-PLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTP---------DDIAIYLLKHGAGLDMKNKKGLTPMEMC 1466
L A + + + VR L + VDA+ + + PL IA G+ D+V LL++ AN NA +D + LH+A + Q ++ +L++ AD TP+ LA+ G++E+ RLL R + I + T LH+A + +LL+ GA A K + H+AA + +I L+ + +++ F +HL A H++ +L+ +D+ + N TA+H+ + H+ +IL N AEIN + + G TPLH+A Q+ K + +G+ T S TP ++ YLL++GA + + G TP+ +
Sbjct: 462 LHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLL-LERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADP--NAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVEN------GADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIA 795 HSP 3 Score: 122.479 bits (306), Expect = 6.986e-28 Identity = 84/311 (27.01%), Postives = 150/311 (48.23%), Query Frame = -1
Query: 471 DARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEIT-RLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMC 1400
D + + PL +A + GH+ V KLL+++ A+ N++ +G T LH+A KN+IK+ +LL+ A + A + +TP+ +AA G++ I LL N D+ +T LHLA R ++ + V +L+ GA ++A ++L H+A+ G+ I+ L L+ ++ D ++ +H+ A E+ +LL + AD + T LH+A +L V++L+E +++ + TPLH+A D +A+ LL++GA K G TP+ +
Sbjct: 354 DVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVET--VRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANS--NATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNN------------------------------DKVAMLLLENGASAKAAAKNGYTPLHIA 630 HSP 4 Score: 104.76 bits (260), Expect = 1.944e-22 Identity = 78/251 (31.08%), Postives = 124/251 (49.40%), Query Frame = -1
Query: 723 LFFAVKNGELNNVRNLCESVGVDARLKG---IYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKIL 1466
L A K G L VR L E G ++G + PL +A + + V LL+E A+ A K+G T LH+A KNQ++I L+ AD NA++ TP+ L+A G EI+ LL +D+ ++ TA+HL ++ V +IL + GA+IN+ + H+A G +++ FL G + + + + +H A H +C+R LL A N + QT L IA +++ V+ L
Sbjct: 561 LHLASKYGNLEVVRLLLER-GTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLL-IENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVK-FLVENGADVGEKTRAS-YTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807 HSP 5 Score: 70.8626 bits (172), Expect = 5.061e-12 Identity = 55/210 (26.19%), Postives = 98/210 (46.67%), Query Frame = -1
Query: 471 GDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSL-MGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMC 1097
G ++ + A R + V LL AG DIN N N HLA+ +GH++++ +KR+ VD+ A+H+ +L + +L+ A++N+++ T L++A + H VK L+++ A L DG TPL +A++ + G + + L + DT A LL++ D+ +K G TP+ +
Sbjct: 29 GRAEGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQ--VDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDT--TAATLLLQNEHNPDVTSKSGFTPLHIA 234
BLAST of SMED30011819 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 127.487 bits (319), Expect = 2.016e-29 Identity = 90/308 (29.22%), Postives = 154/308 (50.00%), Query Frame = -1
Query: 471 GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMK---VKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMC 1385
G PL +A+ +GH VV +L+E ++K K ALH+A K+ LL+ + + + + TP+ +AA G + +LL + A+++ N + LH+A + R N +LL GA I++ K L H AA GH ++++L + +G I ++ ++ A +H+ A +H D R LL ++A ++ T LH+A H+ K+L++ +A+ N R +G TPLHIA K +K +E K + G+ +I IYLL+ GA D++ +G TP+ +
Sbjct: 165 GFTPLAVALQQGHDRVVAVLLEN----DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLL-LEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVV-QGAPI-SAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLA 465 HSP 2 Score: 123.635 bits (309), Expect = 2.924e-28 Identity = 95/343 (27.70%), Postives = 163/343 (47.52%), Query Frame = -1
Query: 471 LFFAVKNGELNNVRNLCES-VGVDARLKGIY-PLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTP---------DDIAIYLLKHGAGLDMKNKKGLTPMEMC 1466
L A + + + VR L + VDA+ + + PL IA G+ D+V LL++ AN NA +D + LH+A + Q ++ +L++ AD TP+ LA+ G++E+ RLL R + I + T LH+A + +LL+ GA A K + H+AA + +I L+ + +++ F +HL A H++ +L+ +D+ + N TA+H+ + H+ +IL N AEIN + + G TPLH+A Q+ K + +G+ T S TP ++ YLL++GA + + G TP+ +
Sbjct: 462 LHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLL-LERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADP--NAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVEN------GADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIA 795 HSP 3 Score: 122.479 bits (306), Expect = 6.986e-28 Identity = 84/311 (27.01%), Postives = 150/311 (48.23%), Query Frame = -1
Query: 471 DARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEIT-RLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMC 1400
D + + PL +A + GH+ V KLL+++ A+ N++ +G T LH+A KN+IK+ +LL+ A + A + +TP+ +AA G++ I LL N D+ +T LHLA R ++ + V +L+ GA ++A ++L H+A+ G+ I+ L L+ ++ D ++ +H+ A E+ +LL + AD + T LH+A +L V++L+E +++ + TPLH+A D +A+ LL++GA K G TP+ +
Sbjct: 354 DVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVET--VRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANS--NATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNN------------------------------DKVAMLLLENGASAKAAAKNGYTPLHIA 630 HSP 4 Score: 104.76 bits (260), Expect = 1.944e-22 Identity = 78/251 (31.08%), Postives = 124/251 (49.40%), Query Frame = -1
Query: 723 LFFAVKNGELNNVRNLCESVGVDARLKG---IYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKIL 1466
L A K G L VR L E G ++G + PL +A + + V LL+E A+ A K+G T LH+A KNQ++I L+ AD NA++ TP+ L+A G EI+ LL +D+ ++ TA+HL ++ V +IL + GA+IN+ + H+A G +++ FL G + + + + +H A H +C+R LL A N + QT L IA +++ V+ L
Sbjct: 561 LHLASKYGNLEVVRLLLER-GTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLL-IENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVK-FLVENGADVGEKTRAS-YTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807 HSP 5 Score: 70.8626 bits (172), Expect = 5.061e-12 Identity = 55/210 (26.19%), Postives = 98/210 (46.67%), Query Frame = -1
Query: 471 GDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSL-MGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMC 1097
G ++ + A R + V LL AG DIN N N HLA+ +GH++++ +KR+ VD+ A+H+ +L + +L+ A++N+++ T L++A + H VK L+++ A L DG TPL +A++ + G + + L + DT A LL++ D+ +K G TP+ +
Sbjct: 29 GRAEGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQ--VDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDT--TAATLLLQNEHNPDVTSKSGFTPLHIA 234
BLAST of SMED30011819 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 127.102 bits (318), Expect = 2.512e-29 Identity = 90/308 (29.22%), Postives = 154/308 (50.00%), Query Frame = -1
Query: 471 GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMK---VKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMC 1385
G PL +A+ +GH VV +L+E ++K K ALH+A K+ LL+ + + + + TP+ +AA G + +LL + A+++ N + LH+A + R N +LL GA I++ K L H AA GH ++++L + +G I ++ ++ A +H+ A +H D R LL ++A ++ T LH+A H+ K+L++ +A+ N R +G TPLHIA K +K +E K + G+ +I IYLL+ GA D++ +G TP+ +
Sbjct: 165 GFTPLAVALQQGHDRVVAVLLEN----DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLL-LEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVV-QGAPI-SAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLA 465 HSP 2 Score: 123.635 bits (309), Expect = 3.176e-28 Identity = 95/343 (27.70%), Postives = 163/343 (47.52%), Query Frame = -1
Query: 471 LFFAVKNGELNNVRNLCES-VGVDARLKGIY-PLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTP---------DDIAIYLLKHGAGLDMKNKKGLTPMEMC 1466
L A + + + VR L + VDA+ + + PL IA G+ D+V LL++ AN NA +D + LH+A + Q ++ +L++ AD TP+ LA+ G++E+ RLL R + I + T LH+A + +LL+ GA A K + H+AA + +I L+ + +++ F +HL A H++ +L+ +D+ + N TA+H+ + H+ +IL N AEIN + + G TPLH+A Q+ K + +G+ T S TP ++ YLL++GA + + G TP+ +
Sbjct: 462 LHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLL-LERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADP--NAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVEN------GADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIA 795 HSP 3 Score: 122.479 bits (306), Expect = 6.790e-28 Identity = 84/311 (27.01%), Postives = 150/311 (48.23%), Query Frame = -1
Query: 471 DARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEIT-RLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMC 1400
D + + PL +A + GH+ V KLL+++ A+ N++ +G T LH+A KN+IK+ +LL+ A + A + +TP+ +AA G++ I LL N D+ +T LHLA R ++ + V +L+ GA ++A ++L H+A+ G+ I+ L L+ ++ D ++ +H+ A E+ +LL + AD + T LH+A +L V++L+E +++ + TPLH+A D +A+ LL++GA K G TP+ +
Sbjct: 354 DVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVET--VRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANS--NATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNN------------------------------DKVAMLLLENGASAKAAAKNGYTPLHIA 630 HSP 4 Score: 104.76 bits (260), Expect = 1.763e-22 Identity = 78/251 (31.08%), Postives = 124/251 (49.40%), Query Frame = -1
Query: 723 LFFAVKNGELNNVRNLCESVGVDARLKG---IYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKIL 1466
L A K G L VR L E G ++G + PL +A + + V LL+E A+ A K+G T LH+A KNQ++I L+ AD NA++ TP+ L+A G EI+ LL +D+ ++ TA+HL ++ V +IL + GA+IN+ + H+A G +++ FL G + + + + +H A H +C+R LL A N + QT L IA +++ V+ L
Sbjct: 561 LHLASKYGNLEVVRLLLER-GTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLL-IENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVK-FLVENGADVGEKTRAS-YTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807 HSP 5 Score: 70.8626 bits (172), Expect = 5.032e-12 Identity = 55/210 (26.19%), Postives = 98/210 (46.67%), Query Frame = -1
Query: 471 GDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSL-MGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMC 1097
G ++ + A R + V LL AG DIN N N HLA+ +GH++++ +KR+ VD+ A+H+ +L + +L+ A++N+++ T L++A + H VK L+++ A L DG TPL +A++ + G + + L + DT A LL++ D+ +K G TP+ +
Sbjct: 29 GRAEGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQ--VDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDT--TAATLLLQNEHNPDVTSKSGFTPLHIA 234
BLAST of SMED30011819 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 127.102 bits (318), Expect = 2.512e-29 Identity = 90/308 (29.22%), Postives = 154/308 (50.00%), Query Frame = -1
Query: 471 GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMK---VKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMC 1385
G PL +A+ +GH VV +L+E ++K K ALH+A K+ LL+ + + + + TP+ +AA G + +LL + A+++ N + LH+A + R N +LL GA I++ K L H AA GH ++++L + +G I ++ ++ A +H+ A +H D R LL ++A ++ T LH+A H+ K+L++ +A+ N R +G TPLHIA K +K +E K + G+ +I IYLL+ GA D++ +G TP+ +
Sbjct: 165 GFTPLAVALQQGHDRVVAVLLEN----DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLL-LEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVV-QGAPI-SAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLA 465 HSP 2 Score: 123.635 bits (309), Expect = 3.176e-28 Identity = 95/343 (27.70%), Postives = 163/343 (47.52%), Query Frame = -1
Query: 471 LFFAVKNGELNNVRNLCES-VGVDARLKGIY-PLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTP---------DDIAIYLLKHGAGLDMKNKKGLTPMEMC 1466
L A + + + VR L + VDA+ + + PL IA G+ D+V LL++ AN NA +D + LH+A + Q ++ +L++ AD TP+ LA+ G++E+ RLL R + I + T LH+A + +LL+ GA A K + H+AA + +I L+ + +++ F +HL A H++ +L+ +D+ + N TA+H+ + H+ +IL N AEIN + + G TPLH+A Q+ K + +G+ T S TP ++ YLL++GA + + G TP+ +
Sbjct: 462 LHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLL-LERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADP--NAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVEN------GADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIA 795 HSP 3 Score: 122.479 bits (306), Expect = 6.790e-28 Identity = 84/311 (27.01%), Postives = 150/311 (48.23%), Query Frame = -1
Query: 471 DARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEIT-RLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMC 1400
D + + PL +A + GH+ V KLL+++ A+ N++ +G T LH+A KN+IK+ +LL+ A + A + +TP+ +AA G++ I LL N D+ +T LHLA R ++ + V +L+ GA ++A ++L H+A+ G+ I+ L L+ ++ D ++ +H+ A E+ +LL + AD + T LH+A +L V++L+E +++ + TPLH+A D +A+ LL++GA K G TP+ +
Sbjct: 354 DVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVET--VRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANS--NATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNN------------------------------DKVAMLLLENGASAKAAAKNGYTPLHIA 630 HSP 4 Score: 104.76 bits (260), Expect = 1.763e-22 Identity = 78/251 (31.08%), Postives = 124/251 (49.40%), Query Frame = -1
Query: 723 LFFAVKNGELNNVRNLCESVGVDARLKG---IYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKIL 1466
L A K G L VR L E G ++G + PL +A + + V LL+E A+ A K+G T LH+A KNQ++I L+ AD NA++ TP+ L+A G EI+ LL +D+ ++ TA+HL ++ V +IL + GA+IN+ + H+A G +++ FL G + + + + +H A H +C+R LL A N + QT L IA +++ V+ L
Sbjct: 561 LHLASKYGNLEVVRLLLER-GTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLL-IENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVK-FLVENGADVGEKTRAS-YTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807 HSP 5 Score: 70.8626 bits (172), Expect = 5.032e-12 Identity = 55/210 (26.19%), Postives = 98/210 (46.67%), Query Frame = -1
Query: 471 GDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSL-MGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMC 1097
G ++ + A R + V LL AG DIN N N HLA+ +GH++++ +KR+ VD+ A+H+ +L + +L+ A++N+++ T L++A + H VK L+++ A L DG TPL +A++ + G + + L + DT A LL++ D+ +K G TP+ +
Sbjct: 29 GRAEGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQ--VDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDT--TAATLLLQNEHNPDVTSKSGFTPLHIA 234
BLAST of SMED30011819 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 126.716 bits (317), Expect = 2.872e-29 Identity = 91/314 (28.98%), Postives = 157/314 (50.00%), Query Frame = -1
Query: 471 GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIA---------IYLLKHGAGLDMKNKKGLTPMEMC 1385
G PL +A+ +GH VV +L+E ++K K ALH+A K+ LL+ + + + + TP+ +AA G + +LL + A+++ N + LH+A + R N +LL GA I++ K L H AA GH ++++L + +G I ++ ++ A +H+ A +H D R LL ++A ++ T LH+A H+ K+L++ +A+ N R +G TPLHIA K +I+ + + + + TE TP +A IYLL+ GA D++ +G TP+ +
Sbjct: 165 GFTPLAVALQQGHDRVVAVLLEN----DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLL-LEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVV-QGAPI-SAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKY------RAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLA 465 HSP 2 Score: 123.635 bits (309), Expect = 3.304e-28 Identity = 95/344 (27.62%), Postives = 163/344 (47.38%), Query Frame = -1
Query: 468 LFFAVKNGELNNVRNLCES-VGVDARLKGIY-PLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTP---------DDIAIYLLKHGAGLDMKNKKGLTPMEMCN 1466
L A + + + VR L + VDA+ + + PL IA G+ D+V LL++ AN NA +D + LH+A + Q ++ +L++ AD TP+ LA+ G++E+ RLL R + I + T LH+A + +LL+ GA A K + H+AA + +I L+ + +++ F +HL A H++ +L+ +D+ + N TA+H+ + H+ +IL N AEIN + + G TPLH+A Q+ K + +G+ T S TP ++ YLL++GA + + G TP+ +
Sbjct: 462 LHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLL-LERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADP--NAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVEN------GADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQ 796 HSP 3 Score: 122.094 bits (305), Expect = 8.029e-28 Identity = 84/311 (27.01%), Postives = 150/311 (48.23%), Query Frame = -1
Query: 471 DARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEIT-RLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMC 1400
D + + PL +A + GH+ V KLL+++ A+ N++ +G T LH+A KN+IK+ +LL+ A + A + +TP+ +AA G++ I LL N D+ +T LHLA R ++ + V +L+ GA ++A ++L H+A+ G+ I+ L L+ ++ D ++ +H+ A E+ +LL + AD + T LH+A +L V++L+E +++ + TPLH+A D +A+ LL++GA K G TP+ +
Sbjct: 354 DVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVET--VRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANS--NATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNN------------------------------DKVAMLLLENGASAKAAAKNGYTPLHIA 630 HSP 4 Score: 104.76 bits (260), Expect = 1.786e-22 Identity = 78/251 (31.08%), Postives = 124/251 (49.40%), Query Frame = -1
Query: 723 LFFAVKNGELNNVRNLCESVGVDARLKG---IYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKIL 1466
L A K G L VR L E G ++G + PL +A + + V LL+E A+ A K+G T LH+A KNQ++I L+ AD NA++ TP+ L+A G EI+ LL +D+ ++ TA+HL ++ V +IL + GA+IN+ + H+A G +++ FL G + + + + +H A H +C+R LL A N + QT L IA +++ V+ L
Sbjct: 561 LHLASKYGNLEVVRLLLER-GTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLL-IENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVK-FLVENGADVGEKTRA-SYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807 HSP 5 Score: 70.4774 bits (171), Expect = 5.491e-12 Identity = 55/210 (26.19%), Postives = 98/210 (46.67%), Query Frame = -1
Query: 471 GDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSL-MGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMC 1097
G ++ + A R + V LL AG DIN N N HLA+ +GH++++ +KR+ VD+ A+H+ +L + +L+ A++N+++ T L++A + H VK L+++ A L DG TPL +A++ + G + + L + DT A LL++ D+ +K G TP+ +
Sbjct: 29 GRAEGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQ--VDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDT--TAATLLLQNEHNPDVTSKSGFTPLHIA 234
BLAST of SMED30011819 vs. Ensembl Fly
Match: mib1 (gene:FBgn0263601 transcript:FBtr0333578) HSP 1 Score: 271.552 bits (693), Expect = 3.225e-76 Identity = 202/676 (29.88%), Postives = 335/676 (49.56%), Query Frame = -1
Query: 120 GDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVN-GLGLVYNPXXXXXXXXXXXKM--FAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECV----EATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVK-IVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPN-PGT--------------KAERLEAPIAPIRQLSPPP-----NIKSRDEELEELRQRIQDMEDEKT-----CIICYDRPINVTF-GCGHVA-CDVCANNLYECHICRESIENKIRL 2039
GD V + D++ + LQ HGGW M G + I D+ V N G +NP +LTK F GD V++ +D +K+LQ+ HGEW +M+ LGK G+V+ ++ D DL+V++ N + T NP V +T SD P I S + L L + + L A + + + V V+ G L A GHI+V+++L+ ++ +DKDGD A+H A ++ + ++L GAD+NA+N + T + +A + G + + + L T S+ DS+ DT LH A+ K + +LLD GADI N FN H AAL+G+ +++ L + IV+ +K+D + A+HL ALNNH + +L+ KA+++ +N LQTALH+AV ++H+ VK+LV++ A++N+ D DGDTPLH A++ + K++ + G G L+ L + + IA +L +GA L +KN+K TP+++C D L ++ N K D E N GT ++ + P++ + S P +I + + E+ Q + + + + C++C D + F CGHV+ C+ CA + +C ICRE++ ++ ++
Sbjct: 232 GDKVTVDLDLEIVQSLQHGHGGWTDGMFECLSNAGMVVGIDEDHDIVVAYNSGNRWTFNPAVLTKVSSPTTAPPEFQVGDIVKICSDVESIKILQRGHGEWADAMQLTLGKIGRVQQVYHDNDLKVEVGNTSWTYNPLAVCKVASSTASDGSCAPVIPSSERLSAILKKLFEPNVSGDATEEFVKAAANGFAARCEEYLAGAAQPSTSSASPSSGPDVNVNGVFAGHTALQAASQNGHIEVIQVLLRHAVDVEIEDKDGDRAVHHAAFGDEAAVIEILAKAGADLNARNKRRQTSLHIAVNKGHLNVVKTLLT-LGCHPSLQDSEGDTPLHDAISKEHDEMLSLLLDFGADITLNNNNGFNALHHAALKGNPSAMKILLTKTNRPWIVEEKKDDGYTALHLAALNNHVEIAELLVHMGKANMDRQNVNLQTALHLAVERQHVQIVKLLVQDGADLNIPDKDGDTPLHEALRHHTLSQLKQLQDVEGFGKLLMGLRNANNKKASA-SIACFLAANGADLTLKNRKQQTPLDLCPDPNLCKTLVK--CYNERKTDDSELPGNVAGTSSSARARAASGSLNQSSSVNMPLSSLAASSTFPAASSSSIFALNGIANEMSQSLHEDPPKSSASLDECLVCSDAKRDTVFKPCGHVSCCETCAPRVKKCLICRETVSSREKI 903 HSP 2 Score: 86.2705 bits (212), Expect = 8.466e-17 Identity = 53/152 (34.87%), Postives = 80/152 (52.63%), Query Frame = -1
Query: 2304 GASLSGLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDA--IVRGPSGNVYRLNSELLSKYGSDTSQ--ESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIA 2747
G G + GD V + + DLE + +Q HGGW D M ECL +V GI D +V SGN + N +L+K S T+ E G +V++ D + + LQ GHGEW D M+ +GK +V + D D+K++ +++ NP A+
Sbjct: 223 GKGPHGFQIGDKVTVDL-DLEIVQSLQHGHGGWTDGMFECLSNA-GMVVGIDEDHDIVVAYNSGNRWTFNPAVLTKVSSPTTAPPEFQVGDIVKICSDVESIKILQRGHGEWADAMQLTLGKIGRVQQVYHDNDLKVEVGNTSWTYNPLAVC 372 HSP 3 Score: 75.0998 bits (183), Expect = 2.762e-13 Identity = 59/215 (27.44%), Postives = 95/215 (44.19%), Query Frame = -1
Query: 2076 IKDLETIIQMQMAHGGW-NDSMNECLIGPCKLVQGIGSDAIVRGPSG-NVYRLNSELLSKYGSDTSQESIS-GYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYN-GSTFVINPKA---IASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINP 2699
I+D + A+ W N S N +G +G+ +V G NVYR + LL + G G V + D + + LQ GHG W D M + + V+ D D+ + YN G+ + NP ++S PP +F++G+ +V+++K LQ HG W+D + L GK + +++ D D+ V W NP
Sbjct: 164 IQDWSSASPRSAAYVIWDNGSKNLYRVG----FEGMADLKVVNDAKGSNVYRDHLPLLGENGPGKGPHGFQIGDKVTVDLDLEIVQSLQHGHGGWTDGMFECLSNAGMVVGIDEDHDIVVAYNSGNRWTFNPAVLTKVSSPTTAPP------EFQVGDIVKICSDVESIKILQRGHGEWADAMQLTLGKIGRVQQVYHDNDLKVEVGNTSWTYNP 368 HSP 4 Score: 64.6994 bits (156), Expect = 3.437e-10 Identity = 24/71 (33.80%), Postives = 50/71 (70.42%), Query Frame = -1
Query: 117 PPNIKS-RDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 326
P N+ + + +++++L+Q++QD++++ C +C+DR N+ F CGH C +C + + C ICR+++E +I L+
Sbjct: 1045 PSNVNNFQMDDVQKLKQQLQDIKEQTMCPVCFDRIKNMVFLCGHGTCQMCGDQIEGCPICRKTVEKRILLF 1115
BLAST of SMED30011819 vs. Ensembl Fly
Match: mib1 (gene:FBgn0263601 transcript:FBtr0075520) HSP 1 Score: 271.552 bits (693), Expect = 7.416e-76 Identity = 203/677 (29.99%), Postives = 336/677 (49.63%), Query Frame = -1
Query: 120 GDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVN-GLGLVYNPXXXXXXXXXXXKM--FAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECV----EATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVK-IVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGS-LMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPN-PGT--------------KAERLEAPIAPIRQLSPPP-----NIKSRDEELEELRQRIQDMEDEKT-----CIICYDRPINVTF-GCGHVA-CDVCANNLYECHICRESIENKIRL 2039
GD V + D++ + LQ HGGW M G + I D+ V N G +NP +LTK F GD V++ +D +K+LQ+ HGEW +M+ LGK G+V+ ++ D DL+V++ N + T NP V +T SD P I S + L L + + L A + + + V V+ G L A GHI+V+++L+ ++ +DKDGD A+H A ++ + ++L GAD+NA+N + T + +A + G + + + L T S+ DS+ DT LH A+ K + +LLD GADI N FN H AAL+G+ +++ L + IV+ +K+D + A+HL ALNNH + +L+ KA+++ +N LQTALH+AV ++H+ VK+LV++ A++N+ D DGDTPLH A++ + K++ + G G LMG+ + + IA +L +GA L +KN+K TP+++C D L ++ N K D E N GT ++ + P++ + S P +I + + E+ Q + + + + C++C D + F CGHV+ C+ CA + +C ICRE++ ++ ++
Sbjct: 343 GDKVTVDLDLEIVQSLQHGHGGWTDGMFECLSNAGMVVGIDEDHDIVVAYNSGNRWTFNPAVLTKVSSPTTAPPEFQVGDIVKICSDVESIKILQRGHGEWADAMQLTLGKIGRVQQVYHDNDLKVEVGNTSWTYNPLAVCKVASSTASDGSCAPVIPSSERLSAILKKLFEPNVSGDATEEFVKAAANGFAARCEEYLAGAAQPSTSSASPSSGPDVNVNGVFAGHTALQAASQNGHIEVIQVLLRHAVDVEIEDKDGDRAVHHAAFGDEAAVIEILAKAGADLNARNKRRQTSLHIAVNKGHLNVVKTLLT-LGCHPSLQDSEGDTPLHDAISKEHDEMLSLLLDFGADITLNNNNGFNALHHAALKGNPSAMKILLTKTNRPWIVEEKKDDGYTALHLAALNNHVEIAELLVHMGKANMDRQNVNLQTALHLAVERQHVQIVKLLVQDGADLNIPDKDGDTPLHEALRHHTLSQLKQLQDVEGFGKLLMGLRNANNKKASA--SIACFLAANGADLTLKNRKQQTPLDLCPDPNLCKTLVK--CYNERKTDDSELPGNVAGTSSSARARAASGSLNQSSSVNMPLSSLAASSTFPAASSSSIFALNGIANEMSQSLHEDPPKSSASLDECLVCSDAKRDTVFKPCGHVSCCETCAPRVKKCLICRETVSSREKI 1014 HSP 2 Score: 86.2705 bits (212), Expect = 8.986e-17 Identity = 53/151 (35.10%), Postives = 80/151 (52.98%), Query Frame = -1
Query: 2307 GASLSGLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDA--IVRGPSGNVYRLNSELLSKYGSDTSQ--ESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAI 2747
G G + GD V + + DLE + +Q HGGW D M ECL +V GI D +V SGN + N +L+K S T+ E G +V++ D + + LQ GHGEW D M+ +GK +V + D D+K++ +++ NP A+
Sbjct: 334 GKGPHGFQIGDKVTVDL-DLEIVQSLQHGHGGWTDGMFECLSNA-GMVVGIDEDHDIVVAYNSGNRWTFNPAVLTKVSSPTTAPPEFQVGDIVKICSDVESIKILQRGHGEWADAMQLTLGKIGRVQQVYHDNDLKVEVGNTSWTYNPLAV 482 HSP 3 Score: 74.7146 bits (182), Expect = 2.982e-13 Identity = 59/215 (27.44%), Postives = 95/215 (44.19%), Query Frame = -1
Query: 2076 IKDLETIIQMQMAHGGW-NDSMNECLIGPCKLVQGIGSDAIVRGPSG-NVYRLNSELLSKYGSDTSQESIS-GYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYN-GSTFVINPKA---IASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINP 2699
I+D + A+ W N S N +G +G+ +V G NVYR + LL + G G V + D + + LQ GHG W D M + + V+ D D+ + YN G+ + NP ++S PP +F++G+ +V+++K LQ HG W+D + L GK + +++ D D+ V W NP
Sbjct: 275 IQDWSSASPRSAAYVIWDNGSKNLYRVG----FEGMADLKVVNDAKGSNVYRDHLPLLGENGPGKGPHGFQIGDKVTVDLDLEIVQSLQHGHGGWTDGMFECLSNAGMVVGIDEDHDIVVAYNSGNRWTFNPAVLTKVSSPTTAPP------EFQVGDIVKICSDVESIKILQRGHGEWADAMQLTLGKIGRVQQVYHDNDLKVEVGNTSWTYNP 479 HSP 4 Score: 65.0846 bits (157), Expect = 3.031e-10 Identity = 24/71 (33.80%), Postives = 50/71 (70.42%), Query Frame = -1
Query: 117 PPNIKS-RDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 326
P N+ + + +++++L+Q++QD++++ C +C+DR N+ F CGH C +C + + C ICR+++E +I L+
Sbjct: 1156 PSNVNNFQMDDVQKLKQQLQDIKEQTMCPVCFDRIKNMVFLCGHGTCQMCGDQIEGCPICRKTVEKRILLF 1226
BLAST of SMED30011819 vs. Ensembl Fly
Match: mib2 (gene:FBgn0086442 transcript:FBtr0336840) HSP 1 Score: 219.164 bits (557), Expect = 1.330e-58 Identity = 170/528 (32.20%), Postives = 261/528 (49.43%), Query Frame = -1
Query: 501 GDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNGLG--LVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNV--TLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCE--SVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDA-GADINALNKKLFNGFHLAALQGH--AKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDD-----IAIYLLKHGAGLDMKN 2039
GD V + ++ +LQQ HGGW RM K G + I GD++V ++P L K F GD V +IND +KV+ LQK HGEW+ M LGK KV +++DGDLR++ ++ TLNP+CV+ S + + E + +M+L ++D G + + KL G L+ VR + VD G + +A ++G++D+V LI K AN+NA DK+GD+ALH A NQ +L+ GA+VN N++ + + + A + R L NA+++I DS DTALH A+ K VE+L +A D N + FN H AAL+G+ A L L R+ +V+ +K+D FAA+HL ALN H + L+ +A+++IRNN QT +AV Q H ++ LV + ++N +D DGD +H+ + K + + + S P+D I IYL + G +++ N
Sbjct: 256 GDRVKVCLEVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAALVKVVS-----FRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCVKLERSP------LATAAERSNSMMDLSHRRADHVMTPLSGLSGSSVAD-----KLVREAAQGHLDFVRQYLDVNPSQVDVMSGGKACIQVASHQGYVDLVSYLISKGANVNAVDKEGDSALHYAAFGNQPATMRVLLQHGAEVNFLNSSHCSALHICAHKKTPHCVREL-LQHNANVNIQDSYGDTALHDAIGKENTEVVELLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRILLLSRQ---LVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLSCDVNAKDEDGDNAMHLCVIKKSNLQSAAEPQPEEAPEIHKFYLSLVHSSIRPEDRLMYSILIYLSRAGCRVELNN 763 HSP 2 Score: 74.3294 bits (181), Expect = 3.821e-13 Identity = 39/140 (27.86%), Postives = 79/140 (56.43%), Query Frame = -1
Query: 2046 QLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKYNGST--FVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKF--GMEWMINPECIDVKDNIL 2450
+LQ GHG WN M + + K+ +H+ D GD++++Y + +P A+ V V F++G+ + I + + +++LQ+ HG W +++ GK ++V+++ DGD+ + + G EW +NP+C+ ++ + L
Sbjct: 270 KLQQGHGGWNPRMVEHL-SKLGTVHRITDKGDIRVQYENCPNRWTFHPAAL--------VKVV--SFRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCVKLERSPL 398 HSP 3 Score: 70.0922 bits (170), Expect = 8.990e-12 Identity = 51/177 (28.81%), Postives = 81/177 (45.76%), Query Frame = -1
Query: 1863 GDVKIKY-----NGSTFVINPKAIASKNEKPPVSE-VCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGM--EWMINPECIDVKDNILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV--NGLGLVYNP 2363
G+V +KY G + + + E PV+ V F +G+R VD + KLQ+ HGGW+ ++ + K + I + GD+ V W +P + VK R GD V + +D K +QLQ+ HG W+ M GK K+ + GD+++ +G NP
Sbjct: 213 GNVDLKYITATCGGHYYKDHMPVLGQTEELQPVAPMVKPSFSVGDRVKVCLEVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAAL-VKVVSFRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNP 388 HSP 4 Score: 65.0846 bits (157), Expect = 3.149e-10 Identity = 45/155 (29.03%), Postives = 79/155 (50.97%), Query Frame = -1
Query: 2277 LKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECL--IGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKY--NGSTFVINPKAIASKNEKPPVS 2729
GD V + + +++ ++++Q HGGWN M E L +G + G + N + + L K S G +V +I D + +QLQ GHGEW + M+ +GK +V+ + DGD++I+ +G + +NPK + K E+ P++
Sbjct: 253 FSVGDRVKVCL-EVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAALVKVVSFRV-----GDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCV--KLERSPLA 399 HSP 5 Score: 60.4622 bits (145), Expect = 7.133e-9 Identity = 32/79 (40.51%), Postives = 42/79 (53.16%), Query Frame = -1
Query: 117 PIRQLSP--PPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 347
P SP P + S D L L ++ + E+ C IC +R +V F CGH AC CA L CH+CR++I KI LY
Sbjct: 972 PTSTSSPSDPTRLPSGDL-LRYLENKVLEFEESHFCGICMERKRDVAFLCGHGACSHCAETLRTCHMCRKTILKKINLY 1049
BLAST of SMED30011819 vs. Ensembl Fly
Match: mib2 (gene:FBgn0086442 transcript:FBtr0081142) HSP 1 Score: 219.164 bits (557), Expect = 1.330e-58 Identity = 170/528 (32.20%), Postives = 261/528 (49.43%), Query Frame = -1
Query: 501 GDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNGLG--LVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNV--TLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCE--SVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDA-GADINALNKKLFNGFHLAALQGH--AKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDD-----IAIYLLKHGAGLDMKN 2039
GD V + ++ +LQQ HGGW RM K G + I GD++V ++P L K F GD V +IND +KV+ LQK HGEW+ M LGK KV +++DGDLR++ ++ TLNP+CV+ S + + E + +M+L ++D G + + KL G L+ VR + VD G + +A ++G++D+V LI K AN+NA DK+GD+ALH A NQ +L+ GA+VN N++ + + + A + R L NA+++I DS DTALH A+ K VE+L +A D N + FN H AAL+G+ A L L R+ +V+ +K+D FAA+HL ALN H + L+ +A+++IRNN QT +AV Q H ++ LV + ++N +D DGD +H+ + K + + + S P+D I IYL + G +++ N
Sbjct: 256 GDRVKVCLEVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAALVKVVS-----FRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCVKLERSP------LATAAERSNSMMDLSHRRADHVMTPLSGLSGSSVAD-----KLVREAAQGHLDFVRQYLDVNPSQVDVMSGGKACIQVASHQGYVDLVSYLISKGANVNAVDKEGDSALHYAAFGNQPATMRVLLQHGAEVNFLNSSHCSALHICAHKKTPHCVREL-LQHNANVNIQDSYGDTALHDAIGKENTEVVELLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRILLLSRQ---LVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLSCDVNAKDEDGDNAMHLCVIKKSNLQSAAEPQPEEAPEIHKFYLSLVHSSIRPEDRLMYSILIYLSRAGCRVELNN 763 HSP 2 Score: 74.3294 bits (181), Expect = 3.821e-13 Identity = 39/140 (27.86%), Postives = 79/140 (56.43%), Query Frame = -1
Query: 2046 QLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKYNGST--FVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKF--GMEWMINPECIDVKDNIL 2450
+LQ GHG WN M + + K+ +H+ D GD++++Y + +P A+ V V F++G+ + I + + +++LQ+ HG W +++ GK ++V+++ DGD+ + + G EW +NP+C+ ++ + L
Sbjct: 270 KLQQGHGGWNPRMVEHL-SKLGTVHRITDKGDIRVQYENCPNRWTFHPAAL--------VKVV--SFRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCVKLERSPL 398 HSP 3 Score: 70.0922 bits (170), Expect = 8.990e-12 Identity = 51/177 (28.81%), Postives = 81/177 (45.76%), Query Frame = -1
Query: 1863 GDVKIKY-----NGSTFVINPKAIASKNEKPPVSE-VCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGM--EWMINPECIDVKDNILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV--NGLGLVYNP 2363
G+V +KY G + + + E PV+ V F +G+R VD + KLQ+ HGGW+ ++ + K + I + GD+ V W +P + VK R GD V + +D K +QLQ+ HG W+ M GK K+ + GD+++ +G NP
Sbjct: 213 GNVDLKYITATCGGHYYKDHMPVLGQTEELQPVAPMVKPSFSVGDRVKVCLEVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAAL-VKVVSFRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNP 388 HSP 4 Score: 65.0846 bits (157), Expect = 3.149e-10 Identity = 45/155 (29.03%), Postives = 79/155 (50.97%), Query Frame = -1
Query: 2277 LKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECL--IGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKY--NGSTFVINPKAIASKNEKPPVS 2729
GD V + + +++ ++++Q HGGWN M E L +G + G + N + + L K S G +V +I D + +QLQ GHGEW + M+ +GK +V+ + DGD++I+ +G + +NPK + K E+ P++
Sbjct: 253 FSVGDRVKVCL-EVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAALVKVVSFRV-----GDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCV--KLERSPLA 399 HSP 5 Score: 60.4622 bits (145), Expect = 7.133e-9 Identity = 32/79 (40.51%), Postives = 42/79 (53.16%), Query Frame = -1
Query: 117 PIRQLSP--PPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 347
P SP P + S D L L ++ + E+ C IC +R +V F CGH AC CA L CH+CR++I KI LY
Sbjct: 972 PTSTSSPSDPTRLPSGDL-LRYLENKVLEFEESHFCGICMERKRDVAFLCGHGACSHCAETLRTCHMCRKTILKKINLY 1049
BLAST of SMED30011819 vs. Ensembl Fly
Match: mib2 (gene:FBgn0086442 transcript:FBtr0336841) HSP 1 Score: 219.164 bits (557), Expect = 1.330e-58 Identity = 170/528 (32.20%), Postives = 261/528 (49.43%), Query Frame = -1
Query: 501 GDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNGLG--LVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNV--TLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCE--SVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDA-GADINALNKKLFNGFHLAALQGH--AKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDD-----IAIYLLKHGAGLDMKN 2039
GD V + ++ +LQQ HGGW RM K G + I GD++V ++P L K F GD V +IND +KV+ LQK HGEW+ M LGK KV +++DGDLR++ ++ TLNP+CV+ S + + E + +M+L ++D G + + KL G L+ VR + VD G + +A ++G++D+V LI K AN+NA DK+GD+ALH A NQ +L+ GA+VN N++ + + + A + R L NA+++I DS DTALH A+ K VE+L +A D N + FN H AAL+G+ A L L R+ +V+ +K+D FAA+HL ALN H + L+ +A+++IRNN QT +AV Q H ++ LV + ++N +D DGD +H+ + K + + + S P+D I IYL + G +++ N
Sbjct: 256 GDRVKVCLEVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAALVKVVS-----FRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCVKLERSP------LATAAERSNSMMDLSHRRADHVMTPLSGLSGSSVAD-----KLVREAAQGHLDFVRQYLDVNPSQVDVMSGGKACIQVASHQGYVDLVSYLISKGANVNAVDKEGDSALHYAAFGNQPATMRVLLQHGAEVNFLNSSHCSALHICAHKKTPHCVREL-LQHNANVNIQDSYGDTALHDAIGKENTEVVELLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRILLLSRQ---LVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLSCDVNAKDEDGDNAMHLCVIKKSNLQSAAEPQPEEAPEIHKFYLSLVHSSIRPEDRLMYSILIYLSRAGCRVELNN 763 HSP 2 Score: 74.3294 bits (181), Expect = 3.821e-13 Identity = 39/140 (27.86%), Postives = 79/140 (56.43%), Query Frame = -1
Query: 2046 QLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKYNGST--FVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKF--GMEWMINPECIDVKDNIL 2450
+LQ GHG WN M + + K+ +H+ D GD++++Y + +P A+ V V F++G+ + I + + +++LQ+ HG W +++ GK ++V+++ DGD+ + + G EW +NP+C+ ++ + L
Sbjct: 270 KLQQGHGGWNPRMVEHL-SKLGTVHRITDKGDIRVQYENCPNRWTFHPAAL--------VKVV--SFRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCVKLERSPL 398 HSP 3 Score: 70.0922 bits (170), Expect = 8.990e-12 Identity = 51/177 (28.81%), Postives = 81/177 (45.76%), Query Frame = -1
Query: 1863 GDVKIKY-----NGSTFVINPKAIASKNEKPPVSE-VCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGM--EWMINPECIDVKDNILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV--NGLGLVYNP 2363
G+V +KY G + + + E PV+ V F +G+R VD + KLQ+ HGGW+ ++ + K + I + GD+ V W +P + VK R GD V + +D K +QLQ+ HG W+ M GK K+ + GD+++ +G NP
Sbjct: 213 GNVDLKYITATCGGHYYKDHMPVLGQTEELQPVAPMVKPSFSVGDRVKVCLEVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAAL-VKVVSFRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNP 388 HSP 4 Score: 65.0846 bits (157), Expect = 3.149e-10 Identity = 45/155 (29.03%), Postives = 79/155 (50.97%), Query Frame = -1
Query: 2277 LKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECL--IGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKY--NGSTFVINPKAIASKNEKPPVS 2729
GD V + + +++ ++++Q HGGWN M E L +G + G + N + + L K S G +V +I D + +QLQ GHGEW + M+ +GK +V+ + DGD++I+ +G + +NPK + K E+ P++
Sbjct: 253 FSVGDRVKVCL-EVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAALVKVVSFRV-----GDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCV--KLERSPLA 399 HSP 5 Score: 60.4622 bits (145), Expect = 7.133e-9 Identity = 32/79 (40.51%), Postives = 42/79 (53.16%), Query Frame = -1
Query: 117 PIRQLSP--PPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 347
P SP P + S D L L ++ + E+ C IC +R +V F CGH AC CA L CH+CR++I KI LY
Sbjct: 972 PTSTSSPSDPTRLPSGDL-LRYLENKVLEFEESHFCGICMERKRDVAFLCGHGACSHCAETLRTCHMCRKTILKKINLY 1049
BLAST of SMED30011819 vs. Ensembl Zebrafish
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:352910]) HSP 1 Score: 279.256 bits (713), Expect = 4.555e-79 Identity = 202/671 (30.10%), Postives = 341/671 (50.82%), Query Frame = -1
Query: 120 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXXXXXX-----------XXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCES--VGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFL-KREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMG--------ILGDMTEGSD--TPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2048
L+ GD V + D++ + LQ HGGW M G + I D+ V +G +NP +LTK+ + F GD V++ D ++KLLQ+ HGEW +M LGK G+V+ +++D DL+V++ + T NP V + + + E LS+L+ + + D EE++K A NG+L V ++ + V V+ + G + A GH+DV+KLL++ +L A+DKDGD A+H A ++ + ++L GAD+NA+N + TP+ +A + G +++ + L S+ DS+ DT LH A+ K R + + +LL+AGAD+ N FN H AAL+G+ + + L K IVD +K+D + A+HL ALNNH + +L+ A+++++N QTALH+AV ++H V++LV A+++++D DGDTPLH A++ + +++ M + + I+G T+G++ + IA +L +GA L ++NKKG +P+++C D L + KE G R P++ S +E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 246 LQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTN--ASGERLSQLLKKLFETQESGDI------------NEELVK---AAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAK-----------CHKEKTSGQVGSR-------------SPSLNSNNETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 861 HSP 2 Score: 80.1073 bits (196), Expect = 1.026e-14 Identity = 64/193 (33.16%), Postives = 94/193 (48.70%), Query Frame = -1
Query: 2193 GLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDA--IVRGPSGNVYRLNSELLSKYGSDTSQESISGY-----------VVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENH 2732
GL+ GD V I + DLE + +Q HGGW D M E L V GI D +V+ PSGN + N +L+K S E +G +V++ D + + LQ GHGEW + M +GK +V + D D+K++ G+++ NP A+ P S V + GER S++ +KKL E
Sbjct: 245 GLQIGDLVNIDL-DLEIVQSLQHGHGGWTDGMFETLT-TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKV--APAGSAVTNAS--GERLSQL-----LKKLFETQ 426 HSP 3 Score: 67.0106 bits (162), Expect = 1.021e-10 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = -1
Query: 117 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 299
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 970 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1030 HSP 4 Score: 65.0846 bits (157), Expect = 3.626e-10 Identity = 63/242 (26.03%), Postives = 107/242 (44.21%), Query Frame = -1
Query: 1893 GNVYRLNSELLSKYGSDTSQESIS-GYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKY-NGSTFVINPKAIASKNEKPPVSEVC-------HKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECIDVKDNILRKGDTVI------LSDDIQKTKQLQQNHGGWVSRMSVFA---GKCGKIEAILPTGDVKVN 2564
G YR + +L + + + + G +V + D + + LQ GHG W D M + + V D D+ ++Y +G+ + NP A+ +K EV +F +G+ ++D +K LQ HG W++ ++ GK + +I+ D D+ V G W NP + + G V LS ++K + Q++ G ++ V A G K+E IL DV VN
Sbjct: 224 GTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNP-AVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV---TKVAPAGSAVTNASGERLSQLLKKLFETQES--GDINEELVKAAANGDLAKVEDILKRPDVDVN 459
BLAST of SMED30011819 vs. Ensembl Zebrafish
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:352910]) HSP 1 Score: 277.33 bits (708), Expect = 1.942e-78 Identity = 206/685 (30.07%), Postives = 344/685 (50.22%), Query Frame = -1
Query: 120 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXXXXXX-----------XXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVE--------------ATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCES--VGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFL-KREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMG--------ILGDMTEGSD--TPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2048
L+ GD V + D++ + LQ HGGW M G + I D+ V +G +NP +LTK+ + F GD V++ D ++KLLQ+ HGEW +M LGK G+V+ +++D DL+V++ + T NP V A ++S S P E LS+L+ + + D EE++K A NG+L V ++ + V V+ + G + A GH+DV+KLL++ +L A+DKDGD A+H A ++ + ++L GAD+NA+N + TP+ +A + G +++ + L S+ DS+ DT LH A+ K R + + +LL+AGAD+ N FN H AAL+G+ + + L K IVD +K+D + A+HL ALNNH + +L+ A+++++N QTALH+AV ++H V++LV A+++++D DGDTPLH A++ + +++ M + + I+G T+G++ + IA +L +GA L ++NKKG +P+++C D L + KE G R P++ S +E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 246 LQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGAVLTESPSVP-----AERLSQLLKKLFETQESGDI------------NEELVK---AAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAK-----------CHKEKTSGQVGSR-------------SPSLNSNNETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 872 HSP 2 Score: 79.337 bits (194), Expect = 1.704e-14 Identity = 53/156 (33.97%), Postives = 79/156 (50.64%), Query Frame = -1
Query: 2304 GLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDA--IVRGPSGNVYRLNSELLSKYGSDTSQESISGY-----------VVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIA 2732
GL+ GD V I + DLE + +Q HGGW D M E L V GI D +V+ PSGN + N +L+K S E +G +V++ D + + LQ GHGEW + M +GK +V + D D+K++ G+++ NP A+
Sbjct: 245 GLQIGDLVNIDL-DLEIVQSLQHGHGGWTDGMFETLT-TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVT 398 HSP 3 Score: 67.0106 bits (162), Expect = 9.573e-11 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = -1
Query: 117 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 299
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 981 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1041 HSP 4 Score: 64.6994 bits (156), Expect = 5.337e-10 Identity = 45/175 (25.71%), Postives = 80/175 (45.71%), Query Frame = -1
Query: 2067 GNVYRLNSELLSKYGSDTSQESIS-GYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKY-NGSTFVINPKAIASKNEKPPVSEVC-------HKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2564
G YR + +L + + + + G +V + D + + LQ GHG W D M + + V D D+ ++Y +G+ + NP A+ +K EV +F +G+ ++D +K LQ HG W++ ++ GK + +I+ D D+ V G W NP +
Sbjct: 224 GTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNP-AVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV 397
BLAST of SMED30011819 vs. Ensembl Zebrafish
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:352910]) HSP 1 Score: 276.559 bits (706), Expect = 4.502e-78 Identity = 203/679 (29.90%), Postives = 341/679 (50.22%), Query Frame = -1
Query: 120 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXXXXXX-----------XXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCES--VGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFL-KREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMG----------------SLMGILGDMTEGSD--TPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2048
L+ GD V + D++ + LQ HGGW M G + I D+ V +G +NP +LTK+ + F GD V++ D ++KLLQ+ HGEW +M LGK G+V+ +++D DL+V++ + T NP V + + + E LS+L+ + + D EE++K A NG+L V ++ + V V+ + G + A GH+DV+KLL++ +L A+DKDGD A+H A ++ + ++L GAD+NA+N + TP+ +A + G +++ + L S+ DS+ DT LH A+ K R + + +LL+AGAD+ N FN H AAL+G+ + + L K IVD +K+D + A+HL ALNNH + +L+ A+++++N QTALH+AV ++H V++LV A+++++D DGDTPLH A++ + +++ M + L I+G T+G++ + IA +L +GA L ++NKKG +P+++C D L + KE G R P++ S +E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 246 LQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTN--ASGERLSQLLKKLFETQESGDI------------NEELVK---AAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTVRTEKCLKLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAK-----------CHKEKTSGQVGSR-------------SPSLNSNNETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 869 HSP 2 Score: 79.7221 bits (195), Expect = 1.065e-14 Identity = 64/193 (33.16%), Postives = 94/193 (48.70%), Query Frame = -1
Query: 2193 GLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDA--IVRGPSGNVYRLNSELLSKYGSDTSQESISGY-----------VVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENH 2732
GL+ GD V I + DLE + +Q HGGW D M E L V GI D +V+ PSGN + N +L+K S E +G +V++ D + + LQ GHGEW + M +GK +V + D D+K++ G+++ NP A+ P S V + GER S++ +KKL E
Sbjct: 245 GLQIGDLVNIDL-DLEIVQSLQHGHGGWTDGMFETLT-TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKV--APAGSAVTNAS--GERLSQL-----LKKLFETQ 426 HSP 3 Score: 66.6254 bits (161), Expect = 1.143e-10 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = -1
Query: 117 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 299
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 978 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1038 HSP 4 Score: 65.0846 bits (157), Expect = 3.993e-10 Identity = 63/242 (26.03%), Postives = 107/242 (44.21%), Query Frame = -1
Query: 1893 GNVYRLNSELLSKYGSDTSQESIS-GYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKY-NGSTFVINPKAIASKNEKPPVSEVC-------HKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECIDVKDNILRKGDTVI------LSDDIQKTKQLQQNHGGWVSRMSVFA---GKCGKIEAILPTGDVKVN 2564
G YR + +L + + + + G +V + D + + LQ GHG W D M + + V D D+ ++Y +G+ + NP A+ +K EV +F +G+ ++D +K LQ HG W++ ++ GK + +I+ D D+ V G W NP + + G V LS ++K + Q++ G ++ V A G K+E IL DV VN
Sbjct: 224 GTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNP-AVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV---TKVAPAGSAVTNASGERLSQLLKKLFETQES--GDINEELVKAAANGDLAKVEDILKRPDVDVN 459
BLAST of SMED30011819 vs. Ensembl Zebrafish
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:ZFIN;Acc:ZDB-GENE-050628-1]) HSP 1 Score: 200.675 bits (509), Expect = 8.094e-53 Identity = 185/665 (27.82%), Postives = 307/665 (46.17%), Query Frame = -1
Query: 123 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEA-----------TDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESV--GVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEIL-LDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGR-----MFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGH-VACDVCANNLYECHICRESIENKIR 2048
++GD V ++ +Q+Q+ HGGW +M+ + G + I GDV+V N + ++P LTK+ ++ + ++V L+ V + LG+ GKV ++ADGDLRV Q T NP C+ A ++ S+S + S++E KL++ Q+ +L +G VR L + VD + +G L +A ++GH++VVK+L++ +++ AKD+DGD ALH I D + + Q+ + + +A + + I + + DS DT LH A+ K + +EIL + D N + FN H AAL+G+ E+ L R ++ D +KED F+A+HL ALNNH D +LL + DINIRNN QT L +AV Q H+ V +LV A++N D DGDT +H ++ +Q+ ++G G++G+ TE + IA +L + GA + N KG +P+++ D ++ I S + K L G+ + + +R++ PN + + + C+IC + + V F C H VAC+ CA+ + +C C+ +I KIR
Sbjct: 201 FQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEI--RDGAL--ITDRGDVRVQYSNIIRWTFHPGALTKARRTHTHTHTHTHTHTHQSYLAQVCLIG------VGAELLALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPGEVDANLMTVENASESSSTVISVLE---KLLS-------------------QSTEQDNPGRLVIEAAHGSAAKVRELLQKHPDKVDIKNQGKTALQVAAHQGHVEVVKVLLQANSSIEAKDEDGDAALHYTA----FGIADSRVITLNKAHYQHLKGLCSLHMAENR-TEHIVFQCVHVCVCVVCVQDSYGDTPLHDAIAKDFRSIIEILTVVPNIDFTQQNNRGFNLLHHAALKGNKLATEMILSR-ARQLADVKKEDGFSALHLAALNNHRDVAEILLKEGRCDINIRNNRNQTPLQLAVTQGHMALVALLVMEGADVNAEDEDGDTAMHTSLSRQQLTTVISSAEGDGAVLYGRLCSSGLMGN-TE-LNVGAAIACFLAQEGADISYANHKGKSPLDLVTDSSVQTLIRSF----AEKHRLHAVSCGTGSSSS-----SSSLRRVHTTPNTMT--------NLAVPSVTGPSECLICSELALLVLFSPCQHSVACEECAHRMKKCIRCQVTITKKIR 808
BLAST of SMED30011819 vs. Ensembl Zebrafish
Match: ank2b (ankyrin 2b, neuronal [Source:ZFIN;Acc:ZDB-GENE-041010-165]) HSP 1 Score: 144.436 bits (363), Expect = 1.780e-34 Identity = 111/353 (31.44%), Postives = 174/353 (49.29%), Query Frame = -1
Query: 471 NQEEVMKLFFAVKNGELNNVRNLCESVGVD--ARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMK---VKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIY---------LLKHGAGLDMKNKKGLTPMEMC 1487
+Q L+ A + L+ VR L E+ G A G PL IA+ +GH VV +L+E + K K ALH+A K+ K LL+ + + Q+ + TP+ +AA G+V + LL +R A + + T LH+A ++ N V +LLD GA I+A + H AA GH +EL L+R G ++ ++ ++ + +H+ A +H +C++ LL +KA ++ TALH+A H K+L++ A N R +G TPLHIA K VK +E K G + +TE TP +A + LL++GA D+ N +G T + M
Sbjct: 116 SQNGFTPLYMASQENHLDVVRYLLENGGNQSIATEDGFTPLAIALQQGHNQVVSILLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLL-LNRGAAVDFTARNGITPLHVASKRGNTNMVHLLLDRGAQIDAKTRDGLTPLHCAARSGHDTAVELLLER-GAPML-ARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLIKYG---------AFIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETALHMA 452 HSP 2 Score: 125.946 bits (315), Expect = 1.061e-28 Identity = 93/316 (29.43%), Postives = 154/316 (48.73%), Query Frame = -1
Query: 462 DARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKV---KQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDD 1400
D L + L +A + GH V KLL++KRAN NA+ +G T LH+A KN++K+ +LLI GA + A + +TPI +AA G + I LL A + + +TALH+A R ++ V LL GA ++A ++ H+A+ G +I++L L+ + D+ + + +H+ A H D VLL A ++ T LH+A L K+L++ A + +G TPLH+A K G + G +IA LL++GA +++ K+G+ P+ + + +
Sbjct: 341 DVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLIKYGAFIQAITESGLTPIHVAAFMGHLNIV-LLLLQNGASPDVSNIRGETALHMAARAGQMEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQH--MAHPDAATANGYTPLHIAAREGHLDVTTVLLEAGAAHSLATKKGFTPLHVASKYGSLEVAKLLLQRRAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHATAKNGYTPLHIAAKKNQMEIATTLLQYGAETNIQTKQGVMPIHLASQE 653 HSP 3 Score: 124.405 bits (311), Expect = 2.452e-28 Identity = 91/324 (28.09%), Postives = 155/324 (47.84%), Query Frame = -1
Query: 468 NLCESVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNA--DLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFG---MGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCN 1424
N +V AR GI PL +A G+ ++V LL+++ A ++AK +DG T LH A +LL+ +GA + A+ N ++P+ +AA VE + L + D+++ D TALH+A ++LLD A+ NA F H+A + K++EL +K + + E IH+ A H + + +LL A ++ N +TALH+A + V+ L+ N A ++ R + TPLHIA ++ + E + + + + G + D+ LL+ GA + KKG TP+ + +
Sbjct: 235 NRGAAVDFTAR-NGITPLHVASKRGNTNMVHLLLDRGAQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPMLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTL---DYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLIKYGA--FIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETALHMAARAGQMEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATANGYTPLHIAAREGHLDVTTVLLEAGAAHSLATKKGFTPLHVAS 552 HSP 4 Score: 117.472 bits (293), Expect = 4.328e-26 Identity = 86/339 (25.37%), Postives = 154/339 (45.43%), Query Frame = -1
Query: 471 GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREG---------------VKIVDSQK----------------EDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQI---EAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMC 1385
G+ P+ +A GH+++V LL++ A+ + + G+TALH+A Q+++ L+ GA V+A+ TP+ +A+ G EI +LL A ++ T LH+A R+ ++ +LL+AGA + KK F H+A+ G ++ +L L+R D+QK ++ + +H+ A N + LL Y A+ NI+ +H+A + H +L++ A++N+ G T LH+A + ++ E K G + +G + + +LLK GA ++ K K G TP+
Sbjct: 412 GLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETALHMAARAGQMEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLL-LQHMAHPDAATANGYTPLHIAAREGHLDVTTVLLEAGAAHSLATKKGFTPLHVASKYGSLEVAKLLLQRRAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHATAKNGYTPLHIAAKKNQMEIATTLLQYGAETNIQTKQGVMPIHLASQEGHSEMAALLLQRGAQVNVTTKSGLTSLHLAAQEDKVGVGEILVKQGANLDQQTKLGYTPLIVACHYGNAKMVNFLLKSGASVNDKTKNGYTPLHQA 749 HSP 5 Score: 115.931 bits (289), Expect = 1.289e-25 Identity = 84/315 (26.67%), Postives = 148/315 (46.98%), Query Frame = -1
Query: 471 GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSD-TP---------DDIAIYLLKHGAGLDMKNKKGLTPMEMC 1385
G PL IA + G+++V LL+ + A ++ ++G T LH+A + + LL+++GA ++A+ + +TP+ AA SG LL R A + + + LH+A + V V+ LL A ++ + H+AA GH ++ +L L + +++ + F +H+ N + +L+ Y A I T +H+A HLN V +L++N A ++ + G+T LH+A + Q+E + + R G + D D TP +I LL+H A D G TP+ +
Sbjct: 214 GFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVHLLLDRGAQIDAKTRDGLTPLHCAARSGHDTAVELL-LERGAPMLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANP--NARALNGFTPLHIACKKNRVKVMELLIKYGAFIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETALHMAARAGQMEVVRCLLR-------NGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATANGYTPLHIA 518 HSP 6 Score: 111.694 bits (278), Expect = 2.462e-24 Identity = 77/282 (27.30%), Postives = 134/282 (47.52%), Query Frame = -1
Query: 624 GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITR-LLATSRNADLSIGDS--------------------DNDT-------ALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKM 1385
G+ L +A EGH+D+V+ L+ + +++++ K G+TALH+A Q + +L +GA++NAQ+ N TP+ +A+ +++ R LL N ++ D +NDT ALH+A RK S +LL + + +K F H+AA G+ + L L R VD + +H+ + + + + +LL A I+ + T LH A H V++L+E A + R +G +PLH+A + +E K +
Sbjct: 53 GLNALHLAAKEGHVDLVQELLGRGSSVDSATKKGNTALHIASLAGQGDVVKILSKRGANINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQSIATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAA--VDFTARNGITPLHVASKRGNTNMVHLLLDRGAQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPMLARTKNGLSPLHMAAQGDHVECVKHL 332 HSP 7 Score: 111.309 bits (277), Expect = 3.151e-24 Identity = 99/373 (26.54%), Postives = 165/373 (44.24%), Query Frame = -1
Query: 471 VMKLFFAVKNGELNNVRNLCE-SVGVDARLK-GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLK--------------------------------REGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILG----DMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMC 1475
+ L A K G N V L + +DA+ + G+ PL A GH V+LL+E+ A + A+ K+G + LH+A + ++ L+ A V+ + +T + +AA G +T+LL R A+ + + T LH+A +K+RV +E+L+ GA I A+ + H+AA GH I+ L L+ R G +VD++ + +H+ + + +++LL + A + T LHIA + HL+ +L+E A +L G TPLH+A K +E K + + G G + D +A+ LL GA K G TP+ +
Sbjct: 248 ITPLHVASKRGNTNMVHLLLDRGAQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPMLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKR-ANPNARALNGFTPLHIACKKNRVKVMELLIKYGAFIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETALHMAARAGQMEVVRCLLRNGA-MVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATANGYTPLHIAAREGHLDVTTVLLEAGAAHSLATKKGFTPLHVASKYGSLEVAKLLLQRRAPPDSAGKNGLTPLHVAAHYDN-QKVALLLLDKGASPHATAKNGYTPLHIA 617 HSP 8 Score: 102.449 bits (254), Expect = 1.485e-21 Identity = 70/248 (28.23%), Postives = 125/248 (50.40%), Query Frame = -1
Query: 654 ARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMK 1397
A G PL IA EGH+DV +L+E A + K G T LH+A +++ LL+ + A ++ N +TP+ +AA + ++ LL + A + T LH+A +K+++ LL GA+ N K+ HLA+ +GH+++ L L+R G + V+ + ++HL A + +L+ A+++ + + T L +A + V L+++ A +N + +G TPLH A +
Sbjct: 507 ATANGYTPLHIAAREGHLDVTTVLLEAGAAHSLATKKGFTPLHVASKYGSLEVAKLLLQRRAPPDSAGKNGLTPLHVAAHYDNQKVA-LLLLDKGASPHATAKNGYTPLHIAAKKNQMEIATTLLQYGAETNIQTKQGVMPIHLASQEGHSEMAALLLQR-GAQ-VNVTTKSGLTSLHLAAQEDKVGVGEILVKQGANLDQQTKLGYTPLIVACHYGNAKMVNFLLKSGASVNDKTKNGYTPLHQAAQ 751 HSP 9 Score: 102.064 bits (253), Expect = 2.331e-21 Identity = 78/277 (28.16%), Postives = 131/277 (47.29%), Query Frame = -1
Query: 642 LFFAVKNGELNNVRNLCESVGVD--ARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQI 1466
L A + G L+ L E+ A KG PL +A G ++V KLL+++RA ++ K+G T LH+A + K+ LL++KGA +A N TP+ +AA +EI L A+ +I +HLA ++ +LL GA +N K HLAA + + E+ +K +G + D Q + + + + + + LL A +N + T LH A Q + + + +L++ A+ N +G+T L IA ++ I
Sbjct: 515 LHIAAREGHLDVTTVLLEAGAAHSLATKKGFTPLHVASKYGSLEVAKLLLQRRAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHATAKNGYTPLHIAAKKNQMEIATTL-LQYGAETNIQTKQGVMPIHLASQEGHSEMAALLLQRGAQVNVTTKSGLTSLHLAAQEDKVGVGEILVK-QGANL-DQQTKLGYTPLIVACHYGNAKMVNFLLKSGASVNDKTKNGYTPLHQAAQQGNTHIINVLLQYGAKPNAITVNGNTALAIARRLGYI 788 HSP 10 Score: 100.138 bits (248), Expect = 7.165e-21 Identity = 71/221 (32.13%), Postives = 114/221 (51.58%), Query Frame = -1
Query: 723 GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKIL 1385
G+ PL +A + + V LL++K A+ +A K+G T LH+A KNQ++I L+ GA+ N Q + PI LA+ G E+ LL R A +++ T+LHLA ++ +V EIL+ GA+++ K + +A G+AK++ FL + G + D K + + +H A + + VLL Y A N TAL IA +++ V L
Sbjct: 577 GLTPLHVAAHYDNQKVALLLLDKGASPHATAKNGYTPLHIAAKKNQMEIATTLLQYGAETNIQTKQGVMPIHLASQEGHSEMAALL-LQRGAQVNVTTKSGLTSLHLAAQEDKVGVGEILVKQGANLDQQTKLGYTPLIVACHYGNAKMVN-FLLKSGASVNDKTK-NGYTPLHQAAQQGNTHIINVLLQYGAKPNAITVNGNTALAIARRLGYISVVDTL 794 HSP 11 Score: 78.5666 bits (192), Expect = 3.543e-14 Identity = 59/208 (28.37%), Postives = 100/208 (48.08%), Query Frame = -1
Query: 471 SDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSL-MGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMC 1091
SD++T+ A R ++ V L G DI N+ N HLAA +GH +++ L R VDS + A+H+ +L D +++L A+IN ++ T L++A + HL+ V+ L+EN ++ DG TPL IA++ + + G + + L DT A LL++ D+++K G TP+ +
Sbjct: 18 SDSNTSFLRAARAGNIDKVLEYLKGGVDIGTSNQNGLNALHLAAKEGHVDLVQELLGRGSS--VDSATKKGNTALHIASLAGQGDVVKILSKRGANINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQSIATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKS--AALLLQNDHNADVQSKSGFTPLHIA 221
BLAST of SMED30011819 vs. Ensembl Xenopus
Match: coa7 (cytochrome c oxidase assembly factor 7 (putative) [Source:Xenbase;Acc:XB-GENE-6258829]) HSP 1 Score: 297.745 bits (761), Expect = 4.956e-86 Identity = 215/725 (29.66%), Postives = 343/725 (47.31%), Query Frame = -1
Query: 117 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNG-LGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEA-----------TDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESV--GVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDA-GADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDI--------AIYLLKHGAGLDMKNKKGLTPMEMC---NDDLL----KARILSLDIPN-STKQDLIEKEPN------PGTKAERLEAPIAP-------------------------------------IRQ------LSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2048
+ GD V D++ +++Q+ HGGW +M+ F G+ G + I GDV+V N ++P LTK + F GD VR++++ VK LQ HGEW M LG+ GKV +F DGD+RV + Q+ T NP C+ + T++ +S+ + S++E L + E L G VR + + VD + +G L +A + G+++VVK+L++ AN++ +D +GDTALH A NQ + +L++KGA+ NN K T + +A + G E+ ++L N +++ DS DT LH A+ + +EIL + D N + FN H +AL+G+ + L+R ++VDS+KED F A+HL LNNH++ + +L+ + D+N+RNN QT LH+AV Q H++ V +LV A++N D DGDTP+HI + +++ G L + E S P +I A +L GA ++ N +G +P+++ DLL I+SLD P S + PN P + A E + +RQ SP + + + +EEL+ R + ME+ TC IC D I + F CGH +C C+ +L C ICR+ I +I+++
Sbjct: 257 FQHGDKVKCLLDVEILREMQEGHGGWNPKMAEFIGQTGTVHRITERGDVRVQYNSETRWTFHPGALTKQNS-----FWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTSYQREEDANLMTTENAKESKNTLVSILEKL----------------------LSQKTDCESPTSLVIEAAQGNTAKVREMLQKYPDKVDIKNQGRTALQVASHLGYMEVVKVLLQANANIDLRDDEGDTALHYAAYGNQAGVVRVLLSKGANAELLNNAKCTALYIAVNKGFTEVVQVLCNP-NCAINMQDSFGDTPLHYAITADFRSIIEILTEVPNIDFTVQNNQGFNLLHHSALKGNVLAVSKILER-ARQLVDSKKEDGFTALHLATLNNHQEVVEILIKEGRCDVNLRNNRNQTPLHLAVAQGHISLVHLLVTEGADVNAEDEDGDTPMHIVFVRQHLKSIDSQQEGNGSSLLTKL-----EASGLPGNIELNVGSAMACFLALSGADINYANHRGKSPLDLIVFPVQDLLCFHVSHAIISLDSPTVSCSLRRVHTTPNTMTNLSPCSAASPAECLVCSELAVLVSFFPCQHSIVCEECSRRMKKCIKCQVCIARKLRQDSTEVECSPSLEVSDQKKVMEELQNRYRQMEERITCPICIDNHIKLVFQCGHGSCTECSTSLTACPICRQLIRERIQIF 947 HSP 2 Score: 95.1301 bits (235), Expect = 2.952e-19 Identity = 57/181 (31.49%), Postives = 92/181 (50.83%), Query Frame = -1
Query: 2067 AIVRGPSGNVYRLNSELLSKYGSDTSQESIS------GYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKYNGST-FVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2585
I P G+ YR + L K +ES G V+ + D + R++Q GHG WN M + IG+ +H+ + GDV+++YN T + +P A+ +N F +G+ + N+D +KKLQ HG W+D ++ G+ +++++F DGDM VS G W NP C+
Sbjct: 223 CITDAPGGHYYRDHLPKLGKPAELQRKESSERHPFQHGDKVKCLLDVEILREMQEGHGGWNPKMAEFIGQ-TGTVHRITERGDVRVQYNSETRWTFHPGALTKQN----------SFWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACL 392 HSP 3 Score: 77.7962 bits (190), Expect = 5.679e-14 Identity = 50/141 (35.46%), Postives = 72/141 (51.06%), Query Frame = -1
Query: 2301 DVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKY--GSDTSQESI-SGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIAS 2714
D V + D+E + +MQ HGGWN M E IG V I VR + NSE + G+ T Q S G VV ++ + ++LQVGHGEW D+M +G+ +VI F DGD+++ G ++ NP + S
Sbjct: 261 DKVKCLLDVEILREMQEGHGGWNPKMAE-FIGQTGTVHRITERGDVR------VQYNSETRWTFHPGALTKQNSFWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTS 394
BLAST of SMED30011819 vs. Ensembl Xenopus
Match: coa7 (cytochrome c oxidase assembly factor 7 (putative) [Source:Xenbase;Acc:XB-GENE-6258829]) HSP 1 Score: 293.508 bits (750), Expect = 2.267e-84 Identity = 211/727 (29.02%), Postives = 339/727 (46.63%), Query Frame = -1
Query: 117 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNG-LGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEA-----------TDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESV--GVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDA-GADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDI--------AIYLLKHGAGLDMKNKKGLTPMEMCNDDLL---------KARILSLDIPN-STKQDLIEKEPN------PGTKAERLEAPIAP-------------------------------------IRQ------LSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2048
+ GD V D++ +++Q+ HGGW +M+ + G + I GDV+V N ++P LTK + F GD VR++++ VK LQ HGEW M LG+ GKV +F DGD+RV + Q+ T NP C+ + T++ +S+ + S++E L + E L G VR + + VD + +G L +A + G+++VVK+L++ AN++ +D +GDTALH A NQ + +L++KGA+ NN K T + +A + G E+ ++L N +++ DS DT LH A+ + +EIL + D N + FN H +AL+G+ + L+R ++VDS+KED F A+HL LNNH++ + +L+ + D+N+RNN QT LH+AV Q H++ V +LV A++N D DGDTP+HI + +++ G L + E S P +I A +L GA ++ N +G +P+++ D + K R LD P S + PN P + A E + +RQ SP + + + +EEL+ R + ME+ TC IC D I + F CGH +C C+ +L C ICR+ I +I+++
Sbjct: 268 FQHGDKVKCLLDVEILREMQEGHGGWNPKMAEVMARTGTVHRITERGDVRVQYNSETRWTFHPGALTKQNS-----FWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTSYQREEDANLMTTENAKESKNTLVSILEKL----------------------LSQKTDCESPTSLVIEAAQGNTAKVREMLQKYPDKVDIKNQGRTALQVASHLGYMEVVKVLLQANANIDLRDDEGDTALHYAAYGNQAGVVRVLLSKGANAELLNNAKCTALYIAVNKGFTEVVQVLCNP-NCAINMQDSFGDTPLHYAITADFRSIIEILTEVPNIDFTVQNNQGFNLLHHSALKGNVLAVSKILER-ARQLVDSKKEDGFTALHLATLNNHQEVVEILIKEGRCDVNLRNNRNQTPLHLAVAQGHISLVHLLVTEGADVNAEDEDGDTPMHIVFVRQHLKSIDSQQEGNGSSLLTKL-----EASGLPGNIELNVGSAMACFLALSGADINYANHRGKSPLDLIADGRITHLVKNFAQKFRYQRLDSPTVSCSLRRVHTTPNTMTNLSPCSAASPAECLVCSELAVLVSFFPCQHSIVCEECSRRMKKCIKCQVCIARKLRQDSTEVECSPSLEVSDQKKVMEELQNRYRQMEERITCPICIDNHIKLVFQCGHGSCTECSTSLTACPICRQLIRERIQIF 960 HSP 2 Score: 93.2041 bits (230), Expect = 1.202e-18 Identity = 55/181 (30.39%), Postives = 92/181 (50.83%), Query Frame = -1
Query: 2067 AIVRGPSGNVYRLNSELLSKYGSDTSQESIS------GYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKYNGST-FVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2585
I P G+ YR + L K +ES G V+ + D + R++Q GHG WN M +++ + +H+ + GDV+++YN T + +P A+ +N F +G+ + N+D +KKLQ HG W+D ++ G+ +++++F DGDM VS G W NP C+
Sbjct: 234 CITDAPGGHYYRDHLPKLGKPAELQRKESSERHPFQHGDKVKCLLDVEILREMQEGHGGWNPKMAEVMAR-TGTVHRITERGDVRVQYNSETRWTFHPGALTKQNS----------FWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACL 403 HSP 3 Score: 74.7146 bits (182), Expect = 6.554e-13 Identity = 48/141 (34.04%), Postives = 72/141 (51.06%), Query Frame = -1
Query: 2301 DVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKY--GSDTSQESI-SGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIAS 2714
D V + D+E + +MQ HGGWN M E ++ V I VR + NSE + G+ T Q S G VV ++ + ++LQVGHGEW D+M +G+ +VI F DGD+++ G ++ NP + S
Sbjct: 272 DKVKCLLDVEILREMQEGHGGWNPKMAE-VMARTGTVHRITERGDVR------VQYNSETRWTFHPGALTKQNSFWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTS 405
BLAST of SMED30011819 vs. Ensembl Xenopus
Match: coa7 (cytochrome c oxidase assembly factor 7 (putative) [Source:Xenbase;Acc:XB-GENE-6258829]) HSP 1 Score: 292.352 bits (747), Expect = 5.135e-84 Identity = 208/712 (29.21%), Postives = 342/712 (48.03%), Query Frame = -1
Query: 117 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNG-LGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEA-----------TDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESV--GVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDA-GADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDI--------AIYLLKHGAGLDMKNKKGLTPMEMCNDDLL---------KARILSLDIP--NSTKQDLIEKEP-NPGTKAERLE-APIAPIRQLSPPPN-------------------------------IKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2048
+ GD V D++ +++Q+ HGGW +M+ F G+ G + I GDV+V N ++P LTK + F GD VR++++ VK LQ HGEW M LG+ GKV +F DGD+RV + Q+ T NP C+ + T++ +S+ + S++E L + E L G VR + + VD + +G L +A + G+++VVK+L++ AN++ +D +GDTALH A NQ + +L++KGA+ NN K T + +A + G E+ ++L N +++ DS DT LH A+ + +EIL + D N + FN H +AL+G+ + L+R ++VDS+KED F A+HL LNNH++ + +L+ + D+N+RNN QT LH+AV Q H++ V +LV A++N D DGDTP+HI + +++ G L + E S P +I A +L GA ++ N +G +P+++ D + K R +P ++T + P + + AE L + +A + P + + + + +EEL+ R + ME+ TC IC D I + F CGH +C C+ +L C ICR+ I +I+++
Sbjct: 274 FQHGDKVKCLLDVEILREMQEGHGGWNPKMAEFIGQTGTVHRITERGDVRVQYNSETRWTFHPGALTKQNS-----FWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTSYQREEDANLMTTENAKESKNTLVSILEKL----------------------LSQKTDCESPTSLVIEAAQGNTAKVREMLQKYPDKVDIKNQGRTALQVASHLGYMEVVKVLLQANANIDLRDDEGDTALHYAAYGNQAGVVRVLLSKGANAELLNNAKCTALYIAVNKGFTEVVQVLCNP-NCAINMQDSFGDTPLHYAITADFRSIIEILTEVPNIDFTVQNNQGFNLLHHSALKGNVLAVSKILER-ARQLVDSKKEDGFTALHLATLNNHQEVVEILIKEGRCDVNLRNNRNQTPLHLAVAQGHISLVHLLVTEGADVNAEDEDGDTPMHIVFVRQHLKSIDSQQEGNGSSLLTKL-----EASGLPGNIELNVGSAMACFLALSGADINYANHRGKSPLDLIADGRITHLVKNFAQKFRYQRAILPYIHTTPNTMTNLSPCSAASPAECLVCSELAVLVSFFPCQHSIVCEECSRRMKKCIKCQVCIARKLRQGGLEVSDQKKVMEELQNRYRQMEERITCPICIDNHIKLVFQCGHGSCTECSTSLTACPICRQLIRERIQIF 951 HSP 2 Score: 94.7449 bits (234), Expect = 3.861e-19 Identity = 57/181 (31.49%), Postives = 92/181 (50.83%), Query Frame = -1
Query: 2067 AIVRGPSGNVYRLNSELLSKYGSDTSQESIS------GYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKYNGST-FVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2585
I P G+ YR + L K +ES G V+ + D + R++Q GHG WN M + IG+ +H+ + GDV+++YN T + +P A+ +N F +G+ + N+D +KKLQ HG W+D ++ G+ +++++F DGDM VS G W NP C+
Sbjct: 240 CITDAPGGHYYRDHLPKLGKPAELQRKESSERHPFQHGDKVKCLLDVEILREMQEGHGGWNPKMAEFIGQ-TGTVHRITERGDVRVQYNSETRWTFHPGALTKQNS----------FWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACL 409 HSP 3 Score: 77.7962 bits (190), Expect = 6.693e-14 Identity = 50/141 (35.46%), Postives = 72/141 (51.06%), Query Frame = -1
Query: 2301 DVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKY--GSDTSQESI-SGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIAS 2714
D V + D+E + +MQ HGGWN M E IG V I VR + NSE + G+ T Q S G VV ++ + ++LQVGHGEW D+M +G+ +VI F DGD+++ G ++ NP + S
Sbjct: 278 DKVKCLLDVEILREMQEGHGGWNPKMAE-FIGQTGTVHRITERGDVR------VQYNSETRWTFHPGALTKQNSFWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTS 411
BLAST of SMED30011819 vs. Ensembl Xenopus
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:100170182]) HSP 1 Score: 275.404 bits (703), Expect = 1.494e-77 Identity = 200/680 (29.41%), Postives = 339/680 (49.85%), Query Frame = -1
Query: 120 GDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXX-----------XXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDI----------FSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLC--ESVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFL-KREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSL---------MGILGDMTEGSD--TPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQ-DLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2039
GD V + +++ + LQ HGGW M G + I D+ V +G +NP + + + F GD V++ D ++KLLQ+ HGEW +M LGK G+V+ +++D DL+V++ + T NP V S + + F ++E LS+L+ + + D EE++K A NG++ V +L + V V+ + G + A GH+D++KLL++ ++ A+DKDGD A+H A ++ + ++L GAD+NA+N + TP+ +A + G +++ + L S+ DS+ DT LH A+ K R + + +LL+AGAD+ N FN H AAL+G+ + + L K IVD +K+D + A+HL ALNNH + +L+ A+++I+N QTALH+AV ++H V++LV A+++++D DGDTPLH A++ + +++ M +G + I+G T+G++ + IA +L +GA L ++NKKG +P+++C D PN K KE + G P I + E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 249 GDLVNIDLELEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSPFQVGDLVQICYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVCGTSWTYNPAAVSRVASVGSAISNATGGKLDYNLSFKLVERLSQLLKKLFETQESGDL------------NEELVK---AAANGDVAKVEDLLKRQDVDVNGQCAGHTAMQAASQNGHVDILKLLLKHSVDVEAEDKDGDRAVHHAAFGDEGTVVEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKKL-LDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDTTWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPD------------PNLCKALAKCHKEKSSGQVGSH------------SPSMISNDSETLEE-------------CMVCSDLKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 875 HSP 2 Score: 76.6406 bits (187), Expect = 1.727e-13 Identity = 52/156 (33.33%), Postives = 81/156 (51.92%), Query Frame = -1
Query: 2304 GLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDA--IVRGPSGNVYRLNSELLSK----YGSDTSQESISGY-------VVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIA 2732
GL GD V I + +LE + +Q HGGW D M E L V GI D +V+ PSGN + N +L+K D +Q + G +V++ D ++ + LQ GHGEW + M +GK +V + D D+K++ G+++ NP A++
Sbjct: 245 GLLIGDLVNIDL-ELEIVQSLQHGHGGWTDGMFETLT-TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSPFQVGDLVQICYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVCGTSWTYNPAAVS 398 HSP 3 Score: 66.6254 bits (161), Expect = 1.648e-10 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = -1
Query: 117 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 299
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 986 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1046 HSP 4 Score: 59.3066 bits (142), Expect = 2.873e-8 Identity = 41/174 (23.56%), Postives = 77/174 (44.25%), Query Frame = -1
Query: 2067 GNVYRLNSELLSKY-GSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKY-NGSTFVINPKAIASKN------EKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2564
G+ YR + +L + G+ + G +V + + + + LQ GHG W D M + + V D D+ ++Y +G+ + NP + N F++G+ +++ +K LQ HG W++ ++ GK + +I+ D D+ V G W NP +
Sbjct: 224 GSFYRDHCPVLGEQNGNRNPGGLLIGDLVNIDLELEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSPFQVGDLVQICYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVCGTSWTYNPAAV 397
BLAST of SMED30011819 vs. Ensembl Xenopus
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:100170182]) HSP 1 Score: 273.863 bits (699), Expect = 4.025e-77 Identity = 199/670 (29.70%), Postives = 337/670 (50.30%), Query Frame = -1
Query: 120 GDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXX-----------XXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLC--ESVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFL-KREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSL---------MGILGDMTEGSD--TPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQ-DLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2039
GD V + +++ + LQ HGGW M G + I D+ V +G +NP + + + F GD V++ D ++KLLQ+ HGEW +M LGK G+V+ +++D DL+V++ + T NP V S + + + E LS+L+ + + D EE++K A NG++ V +L + V V+ + G + A GH+D++KLL++ ++ A+DKDGD A+H A ++ + ++L GAD+NA+N + TP+ +A + G +++ + L S+ DS+ DT LH A+ K R + + +LL+AGAD+ N FN H AAL+G+ + + L K IVD +K+D + A+HL ALNNH + +L+ A+++I+N QTALH+AV ++H V++LV A+++++D DGDTPLH A++ + +++ M +G + I+G T+G++ + IA +L +GA L ++NKKG +P+++C D PN K KE + G P I + E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 249 GDLVNIDLELEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSPFQVGDLVQICYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVCGTSWTYNPAAVSRVASVGSAISN--ATGERLSQLLKKLFETQESGDL------------NEELVK---AAANGDVAKVEDLLKRQDVDVNGQCAGHTAMQAASQNGHVDILKLLLKHSVDVEAEDKDGDRAVHHAAFGDEGTVVEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKKL-LDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDTTWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPD------------PNLCKALAKCHKEKSSGQVGSH------------SPSMISNDSETLEE-------------CMVCSDLKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 863 HSP 2 Score: 76.6406 bits (187), Expect = 1.588e-13 Identity = 52/156 (33.33%), Postives = 81/156 (51.92%), Query Frame = -1
Query: 2304 GLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDA--IVRGPSGNVYRLNSELLSK----YGSDTSQESISGY-------VVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIA 2732
GL GD V I + +LE + +Q HGGW D M E L V GI D +V+ PSGN + N +L+K D +Q + G +V++ D ++ + LQ GHGEW + M +GK +V + D D+K++ G+++ NP A++
Sbjct: 245 GLLIGDLVNIDL-ELEIVQSLQHGHGGWTDGMFETLTTT-GTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSPFQVGDLVQICYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVCGTSWTYNPAAVS 398 HSP 3 Score: 66.6254 bits (161), Expect = 1.817e-10 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = -1
Query: 117 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 299
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 946 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1006 HSP 4 Score: 59.6918 bits (143), Expect = 2.634e-8 Identity = 41/174 (23.56%), Postives = 77/174 (44.25%), Query Frame = -1
Query: 2067 GNVYRLNSELLSKY-GSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKY-NGSTFVINPKAIASKN------EKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2564
G+ YR + +L + G+ + G +V + + + + LQ GHG W D M + + V D D+ ++Y +G+ + NP + N F++G+ +++ +K LQ HG W++ ++ GK + +I+ D D+ V G W NP +
Sbjct: 224 GSFYRDHCPVLGEQNGNRNPGGLLIGDLVNIDLELEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSPFQVGDLVQICYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVCGTSWTYNPAAV 397
BLAST of SMED30011819 vs. Ensembl Mouse
Match: Mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:MGI Symbol;Acc:MGI:2443157]) HSP 1 Score: 275.404 bits (703), Expect = 8.610e-78 Identity = 199/676 (29.44%), Postives = 336/676 (49.70%), Query Frame = -1
Query: 120 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXX-----------XXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVE----ATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCES--VGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFL-KREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMG---------ILGDMTEGSD--TPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2048
L+ GD V + D++ + LQ HGGW M G + I D+ V +G +NP + + + F GD V++ D ++KLLQ+ HGEW +M LGK G+V+ +++D DL+V++ + T NP V A + S++ + S + L KL EE++K A NG++ V +L + V V+ + G + A GH+D++KLL+++ ++ A+DKDGD A+H A ++ + ++L AD+NA+N + TP+ +A + G +++ + L S+ DS+ DT LH A+ K R + + +LL+AGAD+ N FN H AAL+G+ + + L K IVD +K+D + A+HL ALNNH + +L+ A+++I+N QTALH+AV ++H V++LV A+++++D DGDTPLH A++ + +++ M +G + I+G T+G++ + IA +L +GA L ++NKKG +P+++C D L + KE G R P I + E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 246 LQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVAPAGSAISNASGERLSQL--LKKLFETQESGDL----------------NEELVK---AAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAK-----------CHKEKVSGQVGSR------------SPSMISNDSETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 863 HSP 2 Score: 79.7221 bits (195), Expect = 1.182e-14 Identity = 53/156 (33.97%), Postives = 82/156 (52.56%), Query Frame = -1
Query: 2304 GLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDA--IVRGPSGNVYRLNSELLSK----YGSDTSQESISGY-------VVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIA 2732
GL+ GD V I + DLE + +Q HGGW D M E L V GI D +V+ PSGN + N +L+K D +Q + G +V++ D ++ + LQ GHGEW + M +GK +V + D D+K++ G+++ NP A++
Sbjct: 245 GLQIGDLVNIDL-DLEIVQSLQHGHGGWTDGMFETLTTT-GTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVS 398 HSP 3 Score: 66.2402 bits (160), Expect = 1.643e-10 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = -1
Query: 117 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 299
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 946 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY 1006 HSP 4 Score: 63.1586 bits (152), Expect = 1.451e-9 Identity = 41/174 (23.56%), Postives = 78/174 (44.83%), Query Frame = -1
Query: 2067 GNVYRLNSELLSKYGSDTSQESIS-GYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKY-NGSTFVINPKAIASKN------EKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2564
G+ YR + +L + + + + G +V + D + + LQ GHG W D M + + V D D+ ++Y +G+ + NP + N +F++G+ +++ +K LQ HG W++ ++ GK + +I+ D D+ V G W NP +
Sbjct: 224 GSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV 397
BLAST of SMED30011819 vs. Ensembl Mouse
Match: Mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:MGI Symbol;Acc:MGI:2443157]) HSP 1 Score: 275.404 bits (703), Expect = 8.610e-78 Identity = 199/676 (29.44%), Postives = 336/676 (49.70%), Query Frame = -1
Query: 120 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXX-----------XXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVE----ATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCES--VGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFL-KREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMG---------ILGDMTEGSD--TPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2048
L+ GD V + D++ + LQ HGGW M G + I D+ V +G +NP + + + F GD V++ D ++KLLQ+ HGEW +M LGK G+V+ +++D DL+V++ + T NP V A + S++ + S + L KL EE++K A NG++ V +L + V V+ + G + A GH+D++KLL+++ ++ A+DKDGD A+H A ++ + ++L AD+NA+N + TP+ +A + G +++ + L S+ DS+ DT LH A+ K R + + +LL+AGAD+ N FN H AAL+G+ + + L K IVD +K+D + A+HL ALNNH + +L+ A+++I+N QTALH+AV ++H V++LV A+++++D DGDTPLH A++ + +++ M +G + I+G T+G++ + IA +L +GA L ++NKKG +P+++C D L + KE G R P I + E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 246 LQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVAPAGSAISNASGERLSQL--LKKLFETQESGDL----------------NEELVK---AAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAK-----------CHKEKVSGQVGSR------------SPSMISNDSETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 863 HSP 2 Score: 79.7221 bits (195), Expect = 1.182e-14 Identity = 53/156 (33.97%), Postives = 82/156 (52.56%), Query Frame = -1
Query: 2304 GLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDA--IVRGPSGNVYRLNSELLSK----YGSDTSQESISGY-------VVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIA 2732
GL+ GD V I + DLE + +Q HGGW D M E L V GI D +V+ PSGN + N +L+K D +Q + G +V++ D ++ + LQ GHGEW + M +GK +V + D D+K++ G+++ NP A++
Sbjct: 245 GLQIGDLVNIDL-DLEIVQSLQHGHGGWTDGMFETLTTT-GTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVS 398 HSP 3 Score: 66.2402 bits (160), Expect = 1.643e-10 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = -1
Query: 117 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 299
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 946 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY 1006 HSP 4 Score: 63.1586 bits (152), Expect = 1.451e-9 Identity = 41/174 (23.56%), Postives = 78/174 (44.83%), Query Frame = -1
Query: 2067 GNVYRLNSELLSKYGSDTSQESIS-GYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKY-NGSTFVINPKAIASKN------EKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2564
G+ YR + +L + + + + G +V + D + + LQ GHG W D M + + V D D+ ++Y +G+ + NP + N +F++G+ +++ +K LQ HG W++ ++ GK + +I+ D D+ V G W NP +
Sbjct: 224 GSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV 397
BLAST of SMED30011819 vs. Ensembl Mouse
Match: Mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:MGI Symbol;Acc:MGI:2443157]) HSP 1 Score: 229.565 bits (584), Expect = 3.725e-64 Identity = 165/555 (29.73%), Postives = 284/555 (51.17%), Query Frame = -1
Query: 120 GKKGKVKSLFADGDLRVKIMNQNVTLNPECVE----ATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCES--VGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFL-KREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMG---------ILGDMTEGSD--TPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 1721
GK G+V+ +++D DL+V++ + T NP V A + S++ + S + L KL EE++K A NG++ V +L + V V+ + G + A GH+D++KLL+++ ++ A+DKDGD A+H A ++ + ++L AD+NA+N + TP+ +A + G +++ + L S+ DS+ DT LH A+ K R + + +LL+AGAD+ N FN H AAL+G+ + + L K IVD +K+D + A+HL ALNNH + +L+ A+++I+N QTALH+AV ++H V++LV A+++++D DGDTPLH A++ + +++ M +G + I+G T+G++ + IA +L +GA L ++NKKG +P+++C D L + KE G R P I + E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 2 GKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVAPAGSAISNASGERLSQL--LKKLFETQESGDL----------------NEELVK---AAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAK-----------CHKEKVSGQVGSR------------SPSMISNDSETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 498 HSP 2 Score: 66.2402 bits (160), Expect = 1.348e-10 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = -1
Query: 117 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 299
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 581 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY 641
BLAST of SMED30011819 vs. Ensembl Mouse
Match: Mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:MGI Symbol;Acc:MGI:2679684]) HSP 1 Score: 226.868 bits (577), Expect = 1.666e-61 Identity = 179/660 (27.12%), Postives = 296/660 (44.85%), Query Frame = -1
Query: 123 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNGLGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCE--SVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNS--VEILLDA-GADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQI------EAFKKMGRMFGMGSLM--GILG--DMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGH-VACDVCANNLYECHICRESIENKIR 2048
++GD V D + +Q+ HGGW RM+ + F GD VR+I D VK LQ HGEW M LG+ GKV +F DG+LRV + Q T +P C+ A + D+ D+ +++ D+ + + E +L G + +L VD + +G L +A G +++V+LL++ RA+++ D +G+T LH NQ + +L++ G V+A+N + T + +A G +E+ ++L R D+++ D+ DT LH A+ S VE+L + G D+ A N + F H A+L+GH + L R ++VD++KED F A+HL ALNNH + +VL+ + D+N+RN LQ+ LH+AV Q HL V +LV+ +N D +GDT LH+A++ Q+ A G + + L G+ G ++T G+ +A +L GA + N +G +P+++ + R+L Q E++ A R + PN + L + C++C + + + F C H C+ CA + +C C+ I K+R
Sbjct: 257 FQRGDKVKCLLDTDVLRDMQEGHGGWNPRMA----------------------------------------EHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKS-----------DPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILC-ERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILAR-ARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGA----AVACFLALEGADVSYANHRGRSPLDLATE----GRVLK--ALQGCAQRFRERQ------AGGGGGVPPGPRHVLSTPNT------VTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLR 841 HSP 2 Score: 63.1586 bits (152), Expect = 1.467e-9 Identity = 29/74 (39.19%), Postives = 42/74 (56.76%), Query Frame = -1
Query: 117 QLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 338
Q+ PP + +EEL+ R + ME+ TC IC D I + F CGH AC C L C ICR+ I ++I+++
Sbjct: 852 QVPGPPR-----QLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIF 920 HSP 3 Score: 58.5362 bits (140), Expect = 4.573e-8 Identity = 23/65 (35.38%), Postives = 41/65 (63.08%), Query Frame = -1
Query: 2067 FKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2261
F +G+ IG++DT+K+LQ HG W+D + G+ ++V++F DG++ V+ G W +P C+
Sbjct: 292 FWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCL 356 HSP 4 Score: 54.6842 bits (130), Expect = 6.728e-7 Identity = 41/173 (23.70%), Postives = 65/173 (37.57%), Query Frame = -1
Query: 2196 DVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQEN 2714
D V + D + + MQ HGGWN M E NS + G VV +I D ++LQ GHGEW D+M +G+ +V+ F DG++++ G + +P + + R E N+D ++ +EN
Sbjct: 261 DKVKCLLDTDVLRDMQEGHGGWNPRMAE---------------------------HNSFWV-------------GDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCL-----------------VAYRPEEDANLDVAERAREN 376
BLAST of SMED30011819 vs. Ensembl Mouse
Match: Mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:MGI Symbol;Acc:MGI:2679684]) HSP 1 Score: 134.42 bits (337), Expect = 1.765e-33 Identity = 90/310 (29.03%), Postives = 146/310 (47.10%), Query Frame = -1
Query: 1128 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNG-LGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCE--SVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLL 2048
++GD V D + +Q+ HGGW RM+ F G+ G + I GDV+V N ++P LTK + F GD VR+I D VK LQ HGEW M LG+ GKV +F DG+LRV + Q T +P C+ A + D+ D+ +++ + E +L G + +L VD + +G L +A G +++V+LL++ RA+++ D +G+T LH NQ + +L++ G V+A+N + T + +A G +E+ ++L
Sbjct: 82 FQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNS-----FWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSV-----------ALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKIL 375 HSP 2 Score: 90.1225 bits (222), Expect = 1.375e-18 Identity = 50/161 (31.06%), Postives = 86/161 (53.42%), Query Frame = -1
Query: 2067 SELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKYNGST-FVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2543
+EL + +D Q G V+ + D R +Q GHG WN M + IG+ + +H+ D GDV++++N T + +P A+ N F +G+ IG++DT+K+LQ HG W+D + G+ ++V++F DG++ V+ G W +P C+
Sbjct: 69 AELQRRVSAD-GQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQ-MGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNS----------FWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCL 217 HSP 3 Score: 70.0922 bits (170), Expect = 3.859e-12 Identity = 41/142 (28.87%), Postives = 71/142 (50.00%), Query Frame = -1
Query: 1863 VSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGME--WMINPECIDVKDNILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNGLGLVYNP 2282
VS F+ G++ + + D ++ +QE HGGW+ + + G+ + I + GD+ V +F E W +P + K N GD V + D+ K+LQ HG W M+ G+ GK+ + G+++V V G ++P
Sbjct: 75 VSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQMGTVHRITDRGDVRV-QFNHETRWTFHPGAL-TKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSP 214 HSP 4 Score: 67.0106 bits (162), Expect = 3.926e-11 Identity = 44/141 (31.21%), Postives = 66/141 (46.81%), Query Frame = -1
Query: 2301 DVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKY--GSDTSQESIS-GYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIAS 2714
D V + D + + MQ HGGWN M E IG V I VR + N E + G+ T S G VV +I D ++LQ GHGEW D+M +G+ +V+ F DG++++ G + +P + +
Sbjct: 86 DKVKCLLDTDVLRDMQEGHGGWNPRMAE-FIGQMGTVHRITDRGDVR------VQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVA 219 HSP 5 Score: 63.929 bits (154), Expect = 4.029e-10 Identity = 75/314 (23.89%), Postives = 138/314 (43.95%), Query Frame = -1
Query: 927 FAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQN-VTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESVGVDARLKGIYPLMIAV----NEGHIDVVKLLIEKRANLN-AKDK------DGDTALHLAVPK---NQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELF 1823
F GDKV+ + D ++ +Q+ HG W M F+G+ G V + GD+RV+ ++ T +P + T +S D+ ++ L + L + + D + +V+K+F +G L V V + P + + ++DV + E +++L+ A DK D + L V N + DLL V+ +N + T + +AA G VE+ RLL +R A + + D + +T LH ++ + +LL AG ++A N H+A +G +++++
Sbjct: 82 FQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQMGTVHRITDRGDVRVQFNHETRWTFHPGAL--TKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVG-----KVVKVF---GDGNLR--------VAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGR-TALQVAAYLGQVELVRLLLQAR-ASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKIL 375
BLAST of SMED30011819 vs. UniProt/SwissProt
Match: sp|Q5ZIJ9|MIB2_CHICK (E3 ubiquitin-protein ligase MIB2 OS=Gallus gallus OX=9031 GN=MIB2 PE=2 SV=1) HSP 1 Score: 288.115 bits (736), Expect = 8.754e-82 Identity = 203/658 (30.85%), Postives = 331/658 (50.30%), Query Frame = -1
Query: 123 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNG-LGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESV--GVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDA-GADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLM-------GILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSL--DIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVT-FGCGH-VACDVCANNLYECHICRESIENKIR 2048
+ GD V DI +++Q+ HGGW +M+ F G+ G + I GDV+V N ++P LTK + F GD VR+I+D VK Q HGEW M LG GKV ++ DGDLRV + +Q+ T NP C+ A D ++ +L N +S + KT+ + + ++ A+ N VR L + VD + +G L IA +GH+DVVK+L++ A +N +D++GDTALH A NQ + +L+ KGA + NN K T + +A S G E+ + L N D+++ DS DT LH A+ +EIL + D N + FN H +AL+G+ ++ L R ++VDS+KED F A+HL ALNNH++ +L+ + D+N++NN QT LH+A++Q H+ V++LV +++N D DGDT +HIA++ +Q+ + R MGS + G LG++ T IA YL + GA ++ N +G +P+++ D RI+ + D ++ + + A +R++ PN + + + C++C + + + F C H + C+ C+ + +C C+ +I K++
Sbjct: 257 FQHGDKVKCLLDIDILREMQEGHGGWNPKMAEFIGQTGTVHRITDRGDVRVQFNSETRWTFHPGALTKLN-----TFWVGDVVRVIDDMETVKRFQPGHGEWTDEMAPTLGHIGKVIKVYGDGDLRVSVGDQSWTFNPACLTAYQRDEEA---------NLMTTENAKESKSTLITVLEKLLSQKTESDHAGCLVIWAALNNAA--KVRELLQKYPDKVDNKNQGRTALQIASYQGHLDVVKILLQAHATVNLRDEEGDTALHYAAFGNQADVARVLMAKGAGADLLNNAKCTALYVAVSQGFTEVVQALC-ELNCDVNLPDSHGDTPLHYAITADYKVIIEILTEVPNIDFTVQNCQGFNLLHYSALKGNKLAIKKILAR-ARQLVDSKKEDGFTALHLAALNNHKEVAEILIKEGRCDVNVKNNRNQTPLHLAIIQGHVGLVQLLVSEGSDVNAEDEDGDTAMHIALERQQLMSVLMEKREGEMGSSLFSKLQASGFLGNVELNVGTA--IACYLAQEGADINYANHRGKSPLDLITD----GRIVQIIKDFSQKFREQQVSSD---------CSAITCSLRRVHTTPNTMT--------NLSVSSVAVPTECLVCSELALLIHFFPCQHSIVCEECSRRMKKCIKCQVTITKKLK 873 HSP 2 Score: 158.688 bits (400), Expect = 2.396e-38 Identity = 185/749 (24.70%), Postives = 325/749 (43.39%), Query Frame = -1
Query: 117 HKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGME--WMINPECIDVKDNILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNGLGLVYNPXXXXXXXXXXXKMF-----APGDKVRLINDKSKVKLLQKNHGEWVSSMEFF--LGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGE--LNNVRNLCESVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLIN-KGADVNAQNNNKITPIMLAASSGS-VEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAG-ADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNI---LQTALHIAVLQKHLNC--VKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFG----MGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSP-------PPNIKSRDEE--LEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2267
H F+ G++ + ++D ++++QE HGGW+ + + G+ + I + GD+ V +F E W +P + K N GD V + DD++ K+ Q HG W M+ G GK+ + GD++V+V +NP LT + + A K LI K+ L QK + + + L KV+ L +V NQ T + D+ ++ +NL ++++G + GNQ +V ++ A G LNN + C + L +AV++G +VV+ L E ++N D GDT LH A+ + I ++L D QN + +A G+ + I ++LA +R + D TALHLA + EIL+ G D+N N + HLA +QGH +++L + EG V+++ ED A+H +AL + L +L K + + +++ LQ + + ++ ++ L + A+IN + G +PL + + ++ K + F I + TP+ + L + + T +C++ L+L I Q I E + ++ + ++L P+ +S D+ +EEL+ R + ME+ TC IC D I + F CGH +C C+ L C ICR++I +I+++
Sbjct: 255 HPFQHGDKVKCLLDIDILREMQEGHGGWNPKMAEFIGQTGTVHRITDRGDVRV-QFNSETRWTFHPGAL-TKLNTFWVGDVVRVIDDMETVKRFQPGHGEWTDEMAPTLGHIGKVIKVYGDGDLRVSVGDQSWTFNPACLTAYQRDEEANLMTTENAKESKSTLITVLEKL-LSQKTESDHAGCLVIWAALNNAAKVRELLQKYPDKVDNKNQGRT------ALQIASYQGHLDVVKILLQAHATVNL------RDEEGDTALHYAAFGNQADVARVLMAKGAGADLLNNAK--CTA------------LYVAVSQGFTEVVQALCELNCDVNLPDSHGDTPLHYAITADYKVIIEILTEVPNIDFTVQNCQGFNLLHYSALKGNKLAIKKILARARQL-VDSKKEDGFTALHLAALNNHKEVAEILIKEGRCDVNVKNNRNQTPLHLAIIQGHVGLVQLLVS-EGSD-VNAEDEDGDTAMH-IALERQQ--LMSVLMEKREGEMGSSLFSKLQASGFLGNVELNVGTAIACYLAQEGADINYANHRGKSPLDLITDGRIVQIIKDFSQKFREQQVSSDCSAITCSLRRVHTTPNTM--------TNLSVSSVAVPTECLVCSE-------LALLIHFFPCQHSIVCEECSRRMKKCIKCQVTITKKLKRDSTEVECSPSSESTDQRKLMEELQNRYRQMEERITCPICIDDQIKLVFQCGHGSCPDCSTALTVCPICRQAIRERIQIF 953 HSP 3 Score: 79.7221 bits (195), Expect = 7.975e-14 Identity = 42/141 (29.79%), Postives = 74/141 (52.48%), Query Frame = -1
Query: 2067 VELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKYNGST-FVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2483
V+ + D R++Q GHG WN M + IG+ +H+ D GDV++++N T + +P A+ N F +G+ I +++T+K+ Q HG W+D + G ++++++ DGD+ VS W NP C+
Sbjct: 263 VKCLLDIDILREMQEGHGGWNPKMAEFIGQ-TGTVHRITDRGDVRVQFNSETRWTFHPGALTKLN----------TFWVGDVVRVIDDMETVKRFQPGHGEWTDEMAPTLGHIGKVIKVYGDGDLRVSVGDQSWTFNPACL 392 HSP 4 Score: 67.3958 bits (163), Expect = 5.848e-10 Identity = 44/141 (31.21%), Postives = 67/141 (47.52%), Query Frame = -1
Query: 2301 DVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKY--GSDTSQESI-SGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIAS 2714
D V + D++ + +MQ HGGWN M E IG V I VR + NSE + G+ T + G VV +I D + ++ Q GHGEW D M +G +VI + DGD+++ ++ NP + +
Sbjct: 261 DKVKCLLDIDILREMQEGHGGWNPKMAE-FIGQTGTVHRITDRGDVR------VQFNSETRWTFHPGALTKLNTFWVGDVVRVIDDMETVKRFQPGHGEWTDEMAPTLGHIGKVIKVYGDGDLRVSVGDQSWTFNPACLTA 394
BLAST of SMED30011819 vs. UniProt/SwissProt
Match: sp|Q804S5|MIB1_DANRE (E3 ubiquitin-protein ligase mib1 OS=Danio rerio OX=7955 GN=mib1 PE=1 SV=1) HSP 1 Score: 279.256 bits (713), Expect = 3.439e-78 Identity = 202/671 (30.10%), Postives = 341/671 (50.82%), Query Frame = -1
Query: 120 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXXXXXX-----------XXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCES--VGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFL-KREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMG--------ILGDMTEGSD--TPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2048
L+ GD V + D++ + LQ HGGW M G + I D+ V +G +NP +LTK+ + F GD V++ D ++KLLQ+ HGEW +M LGK G+V+ +++D DL+V++ + T NP V + + + E LS+L+ + + D EE++K A NG+L V ++ + V V+ + G + A GH+DV+KLL++ +L A+DKDGD A+H A ++ + ++L GAD+NA+N + TP+ +A + G +++ + L S+ DS+ DT LH A+ K R + + +LL+AGAD+ N FN H AAL+G+ + + L K IVD +K+D + A+HL ALNNH + +L+ A+++++N QTALH+AV ++H V++LV A+++++D DGDTPLH A++ + +++ M + + I+G T+G++ + IA +L +GA L ++NKKG +P+++C D L + KE G R P++ S +E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 246 LQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTN--ASGERLSQLLKKLFETQESGDI------------NEELVK---AAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAK-----------CHKEKTSGQVGSR-------------SPSLNSNNETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 861 HSP 2 Score: 80.1073 bits (196), Expect = 7.743e-14 Identity = 64/193 (33.16%), Postives = 94/193 (48.70%), Query Frame = -1
Query: 2193 GLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDA--IVRGPSGNVYRLNSELLSKYGSDTSQESISGY-----------VVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENH 2732
GL+ GD V I + DLE + +Q HGGW D M E L V GI D +V+ PSGN + N +L+K S E +G +V++ D + + LQ GHGEW + M +GK +V + D D+K++ G+++ NP A+ P S V + GER S++ +KKL E
Sbjct: 245 GLQIGDLVNIDL-DLEIVQSLQHGHGGWTDGMFETLT-TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKV--APAGSAVTNAS--GERLSQL-----LKKLFETQ 426 HSP 3 Score: 67.0106 bits (162), Expect = 7.706e-10 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = -1
Query: 117 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 299
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 970 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1030 HSP 4 Score: 65.0846 bits (157), Expect = 2.738e-9 Identity = 63/242 (26.03%), Postives = 107/242 (44.21%), Query Frame = -1
Query: 1893 GNVYRLNSELLSKYGSDTSQESIS-GYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKY-NGSTFVINPKAIASKNEKPPVSEVC-------HKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECIDVKDNILRKGDTVI------LSDDIQKTKQLQQNHGGWVSRMSVFA---GKCGKIEAILPTGDVKVN 2564
G YR + +L + + + + G +V + D + + LQ GHG W D M + + V D D+ ++Y +G+ + NP A+ +K EV +F +G+ ++D +K LQ HG W++ ++ GK + +I+ D D+ V G W NP + + G V LS ++K + Q++ G ++ V A G K+E IL DV VN
Sbjct: 224 GTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNP-AVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV---TKVAPAGSAVTNASGERLSQLLKKLFETQES--GDINEELVKAAANGDLAKVEDILKRPDVDVN 459
BLAST of SMED30011819 vs. UniProt/SwissProt
Match: sp|Q86YT6|MIB1_HUMAN (E3 ubiquitin-protein ligase MIB1 OS=Homo sapiens OX=9606 GN=MIB1 PE=1 SV=1) HSP 1 Score: 277.33 bits (708), Expect = 1.021e-77 Identity = 201/673 (29.87%), Postives = 339/673 (50.37%), Query Frame = -1
Query: 120 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXX-----------XXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCES--VGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFL-KREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMG---------ILGDMTEGSD--TPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQ-DLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2048
L+ GD V + D++ + LQ HGGW M G + I D+ V +G +NP + + + F GD V++ D ++KLLQ+ HGEW +M LGK G+V+ +++D DL+V++ + T NP V S + + + E LS+L+ + + D EE++K A NG++ V +L + V V+ + G + A GH+D++KLL+++ ++ A+DKDGD A+H A ++ + ++L AD+NA+N + TP+ +A + G +++ + L S+ DS+ DT LH A+ K R + + +LL+AGAD+ N FN H AAL+G+ + + L K IVD +K+D + A+HL ALNNH + +L+ A+++I+N QTALH+AV ++H V++LV A+++++D DGDTPLH A++ + +++ M +G + I+G T+G++ + IA +L +GA L ++NKKG +P+++C D PN K KE G R P I + E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 246 LQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVASAGSAISN--ASGERLSQLLKKLFETQESGDL------------NEELVK---AAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPD------------PNLCKALAKCHKEKVSGQVGSR------------SPSMISNDSETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 863 HSP 2 Score: 79.7221 bits (195), Expect = 8.195e-14 Identity = 53/156 (33.97%), Postives = 82/156 (52.56%), Query Frame = -1
Query: 2304 GLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDA--IVRGPSGNVYRLNSELLSK----YGSDTSQESISGY-------VVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIA 2732
GL+ GD V I + DLE + +Q HGGW D M E L V GI D +V+ PSGN + N +L+K D +Q + G +V++ D ++ + LQ GHGEW + M +GK +V + D D+K++ G+++ NP A++
Sbjct: 245 GLQIGDLVNIDL-DLEIVQSLQHGHGGWTDGMFETLTTT-GTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVS 398 HSP 3 Score: 66.2402 bits (160), Expect = 1.159e-9 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = -1
Query: 117 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 299
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 946 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY 1006 HSP 4 Score: 63.5438 bits (153), Expect = 9.646e-9 Identity = 41/174 (23.56%), Postives = 78/174 (44.83%), Query Frame = -1
Query: 2067 GNVYRLNSELLSKYGSDTSQESIS-GYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKY-NGSTFVINPKAIASKN------EKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2564
G+ YR + +L + + + + G +V + D + + LQ GHG W D M + + V D D+ ++Y +G+ + NP + N +F++G+ +++ +K LQ HG W++ ++ GK + +I+ D D+ V G W NP +
Sbjct: 224 GSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV 397
BLAST of SMED30011819 vs. UniProt/SwissProt
Match: sp|Q80SY4|MIB1_MOUSE (E3 ubiquitin-protein ligase MIB1 OS=Mus musculus OX=10090 GN=Mib1 PE=1 SV=1) HSP 1 Score: 275.404 bits (703), Expect = 6.022e-77 Identity = 199/676 (29.44%), Postives = 336/676 (49.70%), Query Frame = -1
Query: 120 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXX-----------XXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVE----ATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCES--VGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFL-KREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMG---------ILGDMTEGSD--TPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2048
L+ GD V + D++ + LQ HGGW M G + I D+ V +G +NP + + + F GD V++ D ++KLLQ+ HGEW +M LGK G+V+ +++D DL+V++ + T NP V A + S++ + S + L KL EE++K A NG++ V +L + V V+ + G + A GH+D++KLL+++ ++ A+DKDGD A+H A ++ + ++L AD+NA+N + TP+ +A + G +++ + L S+ DS+ DT LH A+ K R + + +LL+AGAD+ N FN H AAL+G+ + + L K IVD +K+D + A+HL ALNNH + +L+ A+++I+N QTALH+AV ++H V++LV A+++++D DGDTPLH A++ + +++ M +G + I+G T+G++ + IA +L +GA L ++NKKG +P+++C D L + KE G R P I + E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 246 LQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVAPAGSAISNASGERLSQL--LKKLFETQESGDL----------------NEELVK---AAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAK-----------CHKEKVSGQVGSR------------SPSMISNDSETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 863 HSP 2 Score: 79.7221 bits (195), Expect = 8.265e-14 Identity = 53/156 (33.97%), Postives = 82/156 (52.56%), Query Frame = -1
Query: 2304 GLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDA--IVRGPSGNVYRLNSELLSK----YGSDTSQESISGY-------VVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIA 2732
GL+ GD V I + DLE + +Q HGGW D M E L V GI D +V+ PSGN + N +L+K D +Q + G +V++ D ++ + LQ GHGEW + M +GK +V + D D+K++ G+++ NP A++
Sbjct: 245 GLQIGDLVNIDL-DLEIVQSLQHGHGGWTDGMFETLTTT-GTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVS 398 HSP 3 Score: 66.2402 bits (160), Expect = 1.150e-9 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = -1
Query: 117 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 299
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 946 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY 1006 HSP 4 Score: 63.1586 bits (152), Expect = 1.015e-8 Identity = 41/174 (23.56%), Postives = 78/174 (44.83%), Query Frame = -1
Query: 2067 GNVYRLNSELLSKYGSDTSQESIS-GYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKY-NGSTFVINPKAIASKN------EKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2564
G+ YR + +L + + + + G +V + D + + LQ GHG W D M + + V D D+ ++Y +G+ + NP + N +F++G+ +++ +K LQ HG W++ ++ GK + +I+ D D+ V G W NP +
Sbjct: 224 GSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV 397
BLAST of SMED30011819 vs. UniProt/SwissProt
Match: sp|Q6GNY1|MIB1_XENLA (E3 ubiquitin-protein ligase mib1 OS=Xenopus laevis OX=8355 GN=mib1 PE=2 SV=1) HSP 1 Score: 272.322 bits (695), Expect = 6.534e-76 Identity = 197/679 (29.01%), Postives = 343/679 (50.52%), Query Frame = -1
Query: 126 GDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXX-----------XXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLC--ESVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFL-KREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSL----------MGILGDMTEGSD--TPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPI-----------APIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHV-ACDVCANNLYECHICRESIENKI 2039
GD V + +++ + LQ HGGW M G + I D+ V +G +NP + + + F GD V++ D ++KLLQ+ HGEW +M LGK G+V+ +++D DL+V++ + T NP V S + + + E LS+L+ + + D EE++K A NG++ V +L + V V+ + G + A GH+D++KLL++ ++ A+DKDGD A+H A ++ + ++L GAD+NA+N + TP+ +A + G +++ + L + S+ DS+ DT LH A+ K R + + +LL+AGAD+ N FN H AAL+G+ + + L K IVD +K+D + A+HL ALNNH + +L+ A+++I+N QTALH+AV ++H V++LV A+++++D DGDTPLH A++ + +++ M +G + I+G T+G++ + IA +L +GA L ++NKKG +P+++C D L + S+ Q +E LE + P ++ R ++ ++++Q + C++C D+ V F CGH+ AC+ CA+ + +C CR +E ++
Sbjct: 249 GDLVNIDLELEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSPFQVGDLVQICYDIERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVCGTSWTYNPAAVSRVASVGSAISN--ATGERLSQLLKKLFETQESGDL------------NEELVK---AAANGDVAKVDDLLKRQDVDVNGQCAGHTAMQAASQNGHVDILKLLLKHSVDVEAEDKDGDRAVHHAAFGDEGTVIEVLQRGGADLNARNKRRQTPLHIAVNKGHLQVVKKL-LDFSCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGSANLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDTTTWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLAVRNKKGQSPLDLCPDPNLCKTLAKCHKEKSSGQVGSHSPSMINNDSETLEECMVCSDLKRDTLFGPCGHIATCSLCSPRVKKCLLCKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRV 909 HSP 2 Score: 76.2554 bits (186), Expect = 9.404e-13 Identity = 52/156 (33.33%), Postives = 81/156 (51.92%), Query Frame = -1
Query: 2304 GLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDA--IVRGPSGNVYRLNSELLSK----YGSDTSQESISGY-------VVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIA 2732
GL GD V I + +LE + +Q HGGW D M E L V GI D +V+ PSGN + N +L+K D +Q + G +V++ D ++ + LQ GHGEW + M +GK +V + D D+K++ G+++ NP A++
Sbjct: 245 GLLIGDLVNIDL-ELEIVQSLQHGHGGWTDGMFETLTTT-GTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSPFQVGDLVQICYDIERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVCGTSWTYNPAAVS 398 HSP 3 Score: 66.6254 bits (161), Expect = 8.998e-10 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = -1
Query: 117 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 299
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 947 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1007 HSP 4 Score: 60.4622 bits (145), Expect = 8.239e-8 Identity = 41/174 (23.56%), Postives = 77/174 (44.25%), Query Frame = -1
Query: 2067 GNVYRLNSELLSKY-GSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKY-NGSTFVINPKAIASKN------EKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2564
G+ YR + +L + G+ + G +V + + + + LQ GHG W D M + + V D D+ ++Y +G+ + NP + N F++G+ +++ +K LQ HG W++ ++ GK + +I+ D D+ V G W NP +
Sbjct: 224 GSFYRDHCPVLGEQNGNRNPGGLLIGDLVNIDLELEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSPFQVGDLVQICYDIERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVCGTSWTYNPAAV 397
BLAST of SMED30011819 vs. TrEMBL
Match: A0A556U1X5 (E3 ubiquitin-protein ligase MIB2 OS=Bagarius yarrelli OX=175774 GN=Baya_7512 PE=4 SV=1) HSP 1 Score: 325.479 bits (833), Expect = 3.317e-92 Identity = 229/719 (31.85%), Postives = 357/719 (49.65%), Query Frame = -1
Query: 117 NILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEA-----------TDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESV--GVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEIL-LDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQI-----EAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDL-LKARILSL--------------DIPNSTKQDLIEKEPNPG------------------------TKAERLEAPI-------APIRQ------LSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2054
+ ++GD V D+ +Q+Q+ HGGW +M+ + + G + I GDV+V N + ++P LTK + FA G+ VR+++D VK LQ HGEW SM LG+ GKV ++ADGDLRV Q T NP C+ A D+ ++S + S++E L Q+ +L +G VR L + VD + +G L +A ++GH++VVK+L++ +++ AKD+DGD ALH NQ +I LL++KGA VN NN+ T + +A + G ++ R+L T +AD+++ DS DT LH A+ K + +EIL + D N + FN H AAL+G+ E+ L R ++VD +KED F+A+HL ALNNH + +L+ + D+NIRNN QT L +AV Q H+ V++LV A +N D +GDT +H A+ +Q+ A + ++ S G+LG+ TE + IA +L + GA ++ N KG +P+++ D + L+A PN+ ++ P P A R++ I IRQ SP +I ++ LE+L+ R + ME+ TC IC D I + F CGH +C C+++L C ICR+SI +I+L+
Sbjct: 344 HTFQQGDKVKCLLDVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN-----TFAVGELVRVLDDIESVKRLQAGHGEWTDSMAPALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPGEVDANLMTADNPTESRTTVISVLEKL----------------------LSQSTEQDNPGRLVIEAAHGSAAKVRELVQKHPDKVDIKNQGKTALQVAAHQGHMEVVKVLLQANSSIEAKDEDGDAALHYTAFGNQSEIARLLLSKGASVNLLNNSMCTALHIAVNKGFTDMVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEILTVVRNIDFTQQNNRGFNLLHHAALKGNKLATEMILAR-ARQLVDKKKEDGFSALHLAALNNHYEVAEILIKEGRCDVNIRNNRGQTPLQLAVTQGHMKLVELLVMEGANVNAEDEEGDTAMHTALSRQQLATMLSSAERDASNLYSRLSSSGLLGN-TE-LNIGAAIACFLAQEGADMNYANHKGKSPLDLVTDSIVLQAGTCGTGGTGGSSSSLRRVHTTPNTMTNLVMPSAPGPSECLICSELALLVLFSPCQHSVACEECAHRMKKCIKCQVTITKKIRQDQTEVDSSPNSDIPAQHNLLEQLQSRYRQMEERITCPICIDNHIKLVFQCGHASCMECSSSLKTCPICRQSIRERIQLF 1031 HSP 2 Score: 88.5817 bits (218), Expect = 5.205e-14 Identity = 50/180 (27.78%), Postives = 90/180 (50.00%), Query Frame = -1
Query: 2058 GPSGNVYRLNSELLSKYGSDTSQESISGYV------VELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKY-NGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECIDVK 2573
G G Y+ + L ++ QES G+ V+ + D RQ+Q GHG WN M + I + + +H+ D GDV+++Y N + +P A+ N F +GE + +++++K+LQ HG W+D + G+ ++++++ DGD+ V+ G W NP C+ +
Sbjct: 316 GQGGFYYKEHLPKLGEHAELQRQESADGHTFQQGDKVKCLLDVDILRQMQEGHGGWNPKMAEYICR-IGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN----------TFAVGELVRVLDDIESVKRLQAGHGEWTDSMAPALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQ 484 HSP 3 Score: 75.8702 bits (185), Expect = 4.092e-10 Identity = 42/141 (29.79%), Postives = 74/141 (52.48%), Query Frame = -1
Query: 2298 DVVIAIKDLETIIQMQMAHGGWNDSMNE--CLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASK 2714
D V + D++ + QMQ HGGWN M E C IG + G D V+ + + + L+K + G +V ++ D + ++LQ GHGEW D+M +G+ +V+ + DGD+++ + G T+ NP ++++
Sbjct: 350 DKVKCLLDVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRG-DVRVQYSNNIRWTFHPGALTKVNT-----FAVGELVRVLDDIESVKRLQAGHGEWTDSMAPALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQ 484
BLAST of SMED30011819 vs. TrEMBL
Match: A0A3Q3WPX3 (Mindbomb E3 ubiquitin protein ligase 2 OS=Mola mola OX=94237 PE=4 SV=1) HSP 1 Score: 320.472 bits (820), Expect = 2.898e-91 Identity = 229/714 (32.07%), Postives = 348/714 (48.74%), Query Frame = -1
Query: 117 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEA-----------TDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESV--GVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEIL-LDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLD-----------------IPNSTKQDLIEKEPNPG------------------------TKAERLEAPI-------APIRQ------LSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2048
++GD V D+ +Q+Q+ HGGW +M+ + + G + I GDV+V N + ++P LTK + F G+ VR++ D VK LQ HGEW SM LG+ GKV ++ADGDLRV Q T NP C+ A T++ ++S + S++E L Q+ +L +G VR L + VD + +G L +A ++GH++VVK L++ ++ KD+DGDTALH + NQ +I LL++KGA+VN NN+ T + +A + G ++ R+L T +AD+++ DS DT LH A+ K + +EIL + D N + FN H AAL+G+ E L R ++VD +KED F+A+HL +LNNH D +L+ + DINIRNN QT L +AV Q H + V++LV A++N+ D DGDT +H+A+ Q+ MF + G+LG+ TE S IA +L + GA ++ N KG +P+++ D + I S PN+ ++ P P A R++ I IRQ SP + LE+L+ R + ME+ TC IC D I + F CGH +C C+ L C ICR++I +I+L+
Sbjct: 249 FQQGDKVKCLLDMDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN-----TFGVGELVRVLEDMESVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPLEVDANLMTTENPNESGSTVISVLEKL----------------------LSQSTEQDNPSRLVIEAAHGSAIKVRELVQKYPDKVDIKNQGKTALQVAAHQGHMEVVKALLQANGSIEIKDEDGDTALHYSAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDMVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEILVMVPNIDFTQQNHRGFNLLHHAALKGNKLATEKILAR-ARQLVDVKKEDGFSALHLASLNNHRDVAEILIKEGRCDINIRNNRNQTPLQLAVTQGHTDLVQLLVAEGADVNMEDEDGDTAMHVALLRPQLANI-----MFSPLNTSGLLGN-TELS-VGAAIACFLAQEGADINYANHKGKSPLDLVADGAMLQLIKSFSEKHRYGLSSASLRRVHTTPNTMTNLVLPTSPGPSECLICSELALLVLFCPCQHSVACEECAHRMKKCIKCQVTITKKIRQDQTEVDCSPGTENSEQHNLLEQLQSRYRQMEERITCPICIDNHIKLVFQCGHASCIDCSAALKTCPICRQTIRERIQLF 926 HSP 2 Score: 86.2705 bits (212), Expect = 2.811e-13 Identity = 50/180 (27.78%), Postives = 90/180 (50.00%), Query Frame = -1
Query: 2058 GPSGNVYRLNSELLSKYGSDTSQESISGYV------VELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKY-NGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECIDVK 2573
G G Y+ + L ++ QES G+ V+ + D RQ+Q GHG WN M + I + + +H+ D GDV+++Y N + +P A+ N F +GE + +++++K+LQ HG W+D + G+ ++++++ DGD+ V+ G W NP C+ +
Sbjct: 219 GQGGFYYKDHLPKLGEHAELQHQESADGHSFQQGDKVKCLLDMDILRQMQEGHGGWNPKMAEYICR-IGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN----------TFGVGELVRVLEDMESVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQ 387 HSP 3 Score: 78.1814 bits (191), Expect = 7.821e-11 Identity = 42/141 (29.79%), Postives = 76/141 (53.90%), Query Frame = -1
Query: 2298 DVVIAIKDLETIIQMQMAHGGWNDSMNE--CLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASK 2714
D V + D++ + QMQ HGGWN M E C IG + G D V+ + + + L+K + G +V +++D + ++LQ GHGEW D+M ++G+ +V+ + DGD+++ + G T+ NP ++++
Sbjct: 253 DKVKCLLDMDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRG-DVRVQYSNNIRWTFHPGALTKVNTFGV-----GELVRVLEDMESVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQ 387 HSP 4 Score: 73.9442 bits (180), Expect = 1.659e-9 Identity = 38/136 (27.94%), Postives = 70/136 (51.47%), Query Frame = -1
Query: 1863 HKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFG-MEWMINPECIDVKDNILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNGLGLVYNP 2267
H F+ G++ + ++D ++++QE HGGW+ + Y + + I + GD+ V + W +P + K N G+ V + +D++ K+LQ HG W M+ G+ GK+ + GD++V G +NP
Sbjct: 247 HSFQQGDKVKCLLDMDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGAL-TKVNTFGVGELVRVLEDMESVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNP 381
BLAST of SMED30011819 vs. TrEMBL
Match: A0A3B5A8W5 (Mindbomb E3 ubiquitin protein ligase 2 OS=Stegastes partitus OX=144197 PE=4 SV=1) HSP 1 Score: 320.087 bits (819), Expect = 4.487e-91 Identity = 225/708 (31.78%), Postives = 343/708 (48.45%), Query Frame = -1
Query: 117 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEA-----------TDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESV--GVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDA-GADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLD-----------IPNSTKQDLIEKEPNPG------------------------TKAERLEAPI-------APIRQ------LSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2048
++GD V ++ +Q+Q+ HGGW +M+ + + G + I GDV+V N + ++P LTK + F G+ VR++ D VK LQ HGEW SM LG+ GKV ++ADGDLRV Q T NP C+ A ++ ++S + S++E L Q+ +L G N VR L + VD + +G L +A ++GH++VVK L++ +++ KD+DGD ALH NQ +I LL++KGA+VN NN+ T + +A + G ++ R+L T +AD+++ DS DT LH A+ K N +EIL+ D N + FN H AAL+G+ E L R ++VD +KED F+A+HL ALNNH D +L+ + DINIRNN QT L +AV+Q H + V++LV A++N+ D DGDT +H+A+ Q+ + + G+LG T IA +L + GA ++ N KG +P+++ D + I S PN+ ++ P P A R++ I IRQ SP + LE+L+ R + ME+ TC IC D I + F CGH +C C+ L C ICR++I +I+L+
Sbjct: 259 FQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN-----TFGVGELVRVLEDMESVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPVEVDANLMTAENPNESGSTVISVLEKL----------------------LSQSTEQDNPSRLVIEAAQGSANKVRELVQKYPDKVDIKNQGKTALQVAAHQGHMEVVKALLQANSSIEVKDEDGDAALHYTAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDLVRVL-TEHSADINLQDSYGDTPLHDAIAKDFRNIIEILVVVPNIDFTQQNHRGFNLLHHAALKGNKLATEKILGR-ARQLVDVKKEDGFSALHLAALNNHRDVAEILIKEGRCDINIRNNRNQTPLQLAVMQGHTDLVQLLVAEGADVNMEDEDGDTAMHVALLRPQLANV-----VLSPLNSSGLLGSTELNVGTA--IACFLAQEGADINYANHKGKSPLDLVADGAMLQVIKSFSENSASLRRVHTTPNTMTNLVLPTPPGPSECLICSELALLVLFCPCQHSVACEECAHRMKKCIKCQVTITKKIRQDQTEVDCSPGTENSEQHNLLEQLQSRYRQMEERITCPICIDNHIKLVFQCGHASCIDCSAALKTCPICRQTIRERIQLF 930 HSP 2 Score: 84.3445 bits (207), Expect = 8.487e-13 Identity = 49/180 (27.22%), Postives = 90/180 (50.00%), Query Frame = -1
Query: 2058 GPSGNVYRLNSELLSKYGSDTSQESISGYV------VELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKY-NGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECIDVK 2573
G G Y+ + L ++ QES G+ V+ + + RQ+Q GHG WN M + I + + +H+ D GDV+++Y N + +P A+ N F +GE + +++++K+LQ HG W+D + G+ ++++++ DGD+ V+ G W NP C+ +
Sbjct: 229 GQGGFYYKDHLPKLGEHAELQRQESADGHSFQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICR-IGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN----------TFGVGELVRVLEDMESVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQ 397 HSP 3 Score: 75.8702 bits (185), Expect = 3.489e-10 Identity = 41/141 (29.08%), Postives = 76/141 (53.90%), Query Frame = -1
Query: 2298 DVVIAIKDLETIIQMQMAHGGWNDSMNE--CLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASK 2714
D V + +++ + QMQ HGGWN M E C IG + G D V+ + + + L+K + G +V +++D + ++LQ GHGEW D+M ++G+ +V+ + DGD+++ + G T+ NP ++++
Sbjct: 263 DKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRG-DVRVQYSNNIRWTFHPGALTKVNTFGV-----GELVRVLEDMESVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQ 397
BLAST of SMED30011819 vs. TrEMBL
Match: A0A3Q1FJ50 (Mindbomb E3 ubiquitin protein ligase 2 OS=Acanthochromis polyacanthus OX=80966 PE=4 SV=1) HSP 1 Score: 321.242 bits (822), Expect = 6.729e-91 Identity = 229/717 (31.94%), Postives = 347/717 (48.40%), Query Frame = -1
Query: 117 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEA-----------TDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESVG--VDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDA-GADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIE--AFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEM----------------CNDDLLKARILSLDIPNSTKQDLIEKEPNPGTK--------------------------AERLEAPI-------APIRQ------LSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2048
++GD V ++ +Q+Q+ HGGW +M+ + + G + I GDV+V N + ++P LTK + F G+ VR++ D VK LQ HGEW SM LG+ GKV ++ADGDLRV Q T NP C+ A ++ ++S + S++E L Q+ +L +G N VR L + VD + +G L +A ++GH++VVK L++ +++ KD+DGDTALH NQ +I LL++KGA+VN NN+ T + +A + G ++ R+L T +AD+++ DS DT LH A+ K N +EIL+ D N + FN H AAL+G+ E L R ++VD +KED F+A+HL ALNNH D +L+ + DINIRNN QT L +AV Q H + V++LV A++N+ D DGDT +H+A+ Q+ K G + + G+LG T IA +L GA ++ N KG +P+++ C L A + + +T +L+ P PG A R++ I IRQ SP + LE+L+ R + ME+ TC IC D I + F CGH +C C+ L C ICR++I +I+L+
Sbjct: 324 FQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN-----TFGVGELVRVLEDMESVKRLQAGHGEWTDSMTPVLGQAGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPVEVDANLMTAENPNESGSTVISVLEKL----------------------LSQSTEQDNPSRLVIEAAHGSANKVRELVQKYPDKVDIKNQGKTALQVAAHQGHMEVVKALLQANSSVEVKDEDGDTALHYTAFGNQAEIARLLLSKGANVNILNNSMCTALHIAVNKGFTDVVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRNIIEILVVVPNIDFTYQNHRGFNLLHHAALKGNKLSTEKILAR-ARQLVDVKKEDGFSALHLAALNNHRDVAEILIKEGRCDINIRNNRNQTPLQLAVTQGHTDLVQLLVAEGADVNMEDEDGDTAMHVALLRPQLANVVLKGCGSIPRPLNSSGLLGSTELNVGTA--IACFLAHEGADINYANHKGKSPLDLVADSTMLQLQRLQAITCGQGLSSANLRRVHTTPNTMTNLVLPTP-PGPSECLICSELALLVLFCPCQHSVACEECAHRMKKCIKCQVTITKKIRQDQTEVDCSPGTENSEQHNLLEQLQSRYRQMEERITCPICIDNHIKLVFQCGHASCIDCSAALKTCPICRQTIRERIQLF 1008 HSP 2 Score: 85.8853 bits (211), Expect = 3.165e-13 Identity = 49/180 (27.22%), Postives = 90/180 (50.00%), Query Frame = -1
Query: 2058 GPSGNVYRLNSELLSKYGSDTSQESISGYV------VELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKY-NGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECIDVK 2573
G G Y+ + L ++ QES G+ V+ + + RQ+Q GHG WN M + I + + +H+ D GDV+++Y N + +P A+ N F +GE + +++++K+LQ HG W+D + G+ ++++++ DGD+ V+ G W NP C+ +
Sbjct: 294 GQGGFYYKDHLPKLGEHAELQRQESADGHSFQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICR-IGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN----------TFGVGELVRVLEDMESVKRLQAGHGEWTDSMTPVLGQAGKVLKVYADGDLRVAFGGQTWTFNPACLSAQ 462 HSP 3 Score: 77.0258 bits (188), Expect = 1.713e-10 Identity = 41/141 (29.08%), Postives = 76/141 (53.90%), Query Frame = -1
Query: 2298 DVVIAIKDLETIIQMQMAHGGWNDSMNE--CLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASK 2714
D V + +++ + QMQ HGGWN M E C IG + G D V+ + + + L+K + G +V +++D + ++LQ GHGEW D+M ++G+ +V+ + DGD+++ + G T+ NP ++++
Sbjct: 328 DKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRG-DVRVQYSNNIRWTFHPGALTKVNTFGV-----GELVRVLEDMESVKRLQAGHGEWTDSMTPVLGQAGKVLKVYADGDLRVAFGGQTWTFNPACLSAQ 462
BLAST of SMED30011819 vs. TrEMBL
Match: A0A5N5NKX2 (Uncharacterized protein OS=Pangasianodon hypophthalmus OX=310915 GN=PHYPO_G00235370 PE=4 SV=1) HSP 1 Score: 320.857 bits (821), Expect = 1.026e-90 Identity = 229/731 (31.33%), Postives = 356/731 (48.70%), Query Frame = -1
Query: 117 NILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEA-----------TDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESV--GVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEIL-LDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAF-----KKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLD---------------------------IPNSTKQDLIEKEPNPG------------------------TKAERLEAPI-------APIRQ----LSPPPNIKSRDEE--LEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2054
+ ++GD V D+ +Q+Q+ HGGW +M+ + + G + I GDV+V N + ++P LTK FA G+ VR+++D VK LQ HGEW SM LG+ GKV ++ADGDLRV Q T NP C+ A ++ S+S + S++E KL++ Q+ +L +G VR L + VD + +G L +A ++GH++VVK+L++ +++ AKD+DGD ALH NQ +I LL++KGA VN NN+ T + +A + G ++ R+L T +AD+++ DS DT LH A+ K + +EIL + D N + FN H AAL+G+ E+ L R ++ D +KED F+A+HL ALNNH + +L+ + D+NIRNN QT L +AV Q H+ V++LV A +N D +GDT +H A+ +Q+ + ++ S G+LG+ TE + IA +L + GA ++ N KG +P+++ D ++ I S PN+ ++ P P A R++ I IRQ + PN +S ++ LE+L+ R + ME+ TC IC D I + F CGH +C C+ L C ICR+SI +I+LY
Sbjct: 314 HTFQQGDKVKCLLDVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVH-----TFAVGELVRVLDDIETVKRLQAGHGEWTDSMAPALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPGEVDANLMTAENPSESRTTVISVLE---KLLS-------------------QSTEQDNPGRLVIEAAHGSAAKVRELVQKHPDKVDIKNQGKTALQVAAHQGHMEVVKVLLQANSSIEAKDEDGDAALHYTAFGNQAEIARLLLSKGASVNLLNNSMCTALHIAVNKGFTDVVRVL-TDHSADVNLQDSYGDTPLHDAIAKDFRSIIEILTVVRNIDFTQQNNRGFNLLHHAALKGNKLATEMILAR-ARQLADVKKEDGFSALHLAALNNHYEVAEILIKEGRCDVNIRNNRGQTPLQLAVTQGHMQLVELLVMEGANVNAEDEEGDTAMHTALSRQQLTTTLSSTERDASNLYSRLSGSGLLGN-TE-LNVGAAIACFLAQEGADINYANHKGKSPLDLVTDSIVLQLIRSFSEKYRLQAGTCGTGGSGSSSSSLRRVHTTPNTMTNLVMPSAPGPSECLICSELALLVLFSPCQHSVACEECAHRMKKCIKCQVTITKKIRQDQTEVDSSPNSESSEQHHLLEQLQSRYRQMEERITCPICIDNHIKLVFQCGHASCIDCSATLKTCPICRQSIRERIQLY 1013 HSP 2 Score: 91.6633 bits (226), Expect = 5.180e-15 Identity = 51/180 (28.33%), Postives = 90/180 (50.00%), Query Frame = -1
Query: 2058 GPSGNVYRLNSELLSKYGSDTSQESISGYV------VELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKY-NGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECIDVK 2573
G G Y+ + L ++ QES G+ V+ + D RQ+Q GHG WN M + I + + +H+ D GDV+++Y N + +P A+ H F +GE + +++T+K+LQ HG W+D + G+ ++++++ DGD+ V+ G W NP C+ +
Sbjct: 286 GQGGYYYKEHLPKLGEHAELQRQESADGHTFQQGDKVKCLLDVDILRQMQEGHGGWNPKMAEYICR-IGTVHRITDRGDVRVQYSNNIRWTFHPGALTK----------VHTFAVGELVRVLDDIETVKRLQAGHGEWTDSMAPALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQ 454 HSP 3 Score: 75.485 bits (184), Expect = 5.425e-10 Identity = 44/142 (30.99%), Postives = 74/142 (52.11%), Query Frame = -1
Query: 2298 DVVIAIKDLETIIQMQMAHGGWNDSMNE--CLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESIS-GYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASK 2714
D V + D++ + QMQ HGGWN M E C IG + G D V+ Y N G+ T + + G +V ++ D + ++LQ GHGEW D+M +G+ +V+ + DGD+++ + G T+ NP ++++
Sbjct: 320 DKVKCLLDVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRG-DVRVQ------YSNNIRWTFHPGALTKVHTFAVGELVRVLDDIETVKRLQAGHGEWTDSMAPALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQ 454
BLAST of SMED30011819 vs. Ensembl Cavefish
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:103034310]) HSP 1 Score: 311.612 bits (797), Expect = 8.405e-91 Identity = 224/731 (30.64%), Postives = 349/731 (47.74%), Query Frame = -1
Query: 117 NILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEA-----------TDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESV--GVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEIL-LDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQI-----EAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLD---------------------------IPNSTKQDLIEKEPNPG------------------------TKAERLEAPI-------APIRQ------LSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2054
+ ++GD V ++ +Q+Q+ HGGW +M+ + + G + I GDV+V N + ++P LTK + FA G+ VR+++D VK LQ HGEW SM LG+ GKV ++ADGDLRV Q T NP C+ ++ S+S + S++E L Q+ +L +G VR L + VD + +G L +A ++GH++VVK+L++ +++ AKD+DGD ALH NQ +I LL++KGA+VN NN+ T + +A + G ++ R+L T +AD+++ DS DT LH A+ K + +EIL + D N + FN H AAL+G+ E+ L R ++ D +KED F+A+HL ALNNH D +L+ + D NIRNN QT L +AV Q H+ V +LV A++N D +GDT +H A+ +Q+ + ++ ++ S G+LG+ TE + IA +L + GA ++ N KG +P+++ D I S PN+ + P P A R++ I IRQ SP + + LE+L+ R + ME+ TC IC D I + F CGH +C C+ L C ICR++I +I+L+
Sbjct: 311 HTFQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN-----TFAVGELVRVLDDIESVKRLQAGHGEWTDSMASALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSGQPGEVDANLMTAENPSESGSTVISVLEKL----------------------LSQSTEQDNPGRLVIEAAHGSATKVRELVQKHPDKVDIKNQGKTALQVAAHQGHMEVVKVLLQANSSIEAKDEDGDAALHYTAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEILTVVPNIDFTQQNNRGFNLLHHAALKGNKLATEMILSR-ARQLADVKKEDGFSALHLAALNNHRDVAEILVKEGRCDANIRNNRNQTPLQLAVTQGHVALVALLVSEGADVNAEDEEGDTAMHTALSRQQLTNMLSTSEREASALYCRLSSSGLLGN-TE-LNVGAAIACFLAQEGADINYANHKGKSPLDLVTDSTTLQLIRSFSEKHRLQQLQAITCGTSSSSSSLRRVHTTPNTMTNLAMPSAPGPSECLICSELALLVLFSPCQHSVACEECAHRMKKCIKCQVTITKKIRQDQTEVECSPNSDNSEQHNLLEQLQSRYRQMEERITCPICIDNQIKLVFQCGHASCIDCSAALKTCPICRQTIRERIQLF 1010 HSP 2 Score: 87.4261 bits (215), Expect = 4.857e-17 Identity = 49/177 (27.68%), Postives = 89/177 (50.28%), Query Frame = -1
Query: 2067 GPSGNVYRLNSELLSKYGSDTSQESISGYV------VELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKY-NGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2573
G G Y+ + L ++ QES G+ V+ + + RQ+Q GHG WN M + I + + +H+ D GDV+++Y N + +P A+ N F +GE + +++++K+LQ HG W+D + G+ ++++++ DGD+ V+ G W NP C+
Sbjct: 283 GQGGYYYKEHLPKLGEHAELQRQESADGHTFQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICR-IGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN----------TFAVGELVRVLDDIESVKRLQAGHGEWTDSMASALGQVGKVLKVYADGDLRVAFGGQTWTFNPACL 448 HSP 3 Score: 73.9442 bits (180), Expect = 6.003e-13 Identity = 41/141 (29.08%), Postives = 73/141 (51.77%), Query Frame = -1
Query: 2298 DVVIAIKDLETIIQMQMAHGGWNDSMNE--CLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASK 2714
D V + +++ + QMQ HGGWN M E C IG + G D V+ + + + L+K + G +V ++ D + ++LQ GHGEW D+M +G+ +V+ + DGD+++ + G T+ NP ++ +
Sbjct: 317 DKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRG-DVRVQYSNNIRWTFHPGALTKVNT-----FAVGELVRVLDDIESVKRLQAGHGEWTDSMASALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSGQ 451
BLAST of SMED30011819 vs. Ensembl Cavefish
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:103034310]) HSP 1 Score: 311.612 bits (797), Expect = 9.115e-91 Identity = 224/731 (30.64%), Postives = 349/731 (47.74%), Query Frame = -1
Query: 117 NILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEA-----------TDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESV--GVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEIL-LDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQI-----EAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLD---------------------------IPNSTKQDLIEKEPNPG------------------------TKAERLEAPI-------APIRQ------LSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2054
+ ++GD V ++ +Q+Q+ HGGW +M+ + + G + I GDV+V N + ++P LTK + FA G+ VR+++D VK LQ HGEW SM LG+ GKV ++ADGDLRV Q T NP C+ ++ S+S + S++E L Q+ +L +G VR L + VD + +G L +A ++GH++VVK+L++ +++ AKD+DGD ALH NQ +I LL++KGA+VN NN+ T + +A + G ++ R+L T +AD+++ DS DT LH A+ K + +EIL + D N + FN H AAL+G+ E+ L R ++ D +KED F+A+HL ALNNH D +L+ + D NIRNN QT L +AV Q H+ V +LV A++N D +GDT +H A+ +Q+ + ++ ++ S G+LG+ TE + IA +L + GA ++ N KG +P+++ D I S PN+ + P P A R++ I IRQ SP + + LE+L+ R + ME+ TC IC D I + F CGH +C C+ L C ICR++I +I+L+
Sbjct: 317 HTFQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN-----TFAVGELVRVLDDIESVKRLQAGHGEWTDSMASALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSGQPGEVDANLMTAENPSESGSTVISVLEKL----------------------LSQSTEQDNPGRLVIEAAHGSATKVRELVQKHPDKVDIKNQGKTALQVAAHQGHMEVVKVLLQANSSIEAKDEDGDAALHYTAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEILTVVPNIDFTQQNNRGFNLLHHAALKGNKLATEMILSR-ARQLADVKKEDGFSALHLAALNNHRDVAEILVKEGRCDANIRNNRNQTPLQLAVTQGHVALVALLVSEGADVNAEDEEGDTAMHTALSRQQLTNMLSTSEREASALYCRLSSSGLLGN-TE-LNVGAAIACFLAQEGADINYANHKGKSPLDLVTDSTTLQLIRSFSEKHRLQQLQAITCGTSSSSSSLRRVHTTPNTMTNLAMPSAPGPSECLICSELALLVLFSPCQHSVACEECAHRMKKCIKCQVTITKKIRQDQTEVECSPNSDNSEQHNLLEQLQSRYRQMEERITCPICIDNQIKLVFQCGHASCIDCSAALKTCPICRQTIRERIQLF 1016 HSP 2 Score: 87.0409 bits (214), Expect = 5.173e-17 Identity = 49/177 (27.68%), Postives = 89/177 (50.28%), Query Frame = -1
Query: 2067 GPSGNVYRLNSELLSKYGSDTSQESISGYV------VELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKY-NGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2573
G G Y+ + L ++ QES G+ V+ + + RQ+Q GHG WN M + I + + +H+ D GDV+++Y N + +P A+ N F +GE + +++++K+LQ HG W+D + G+ ++++++ DGD+ V+ G W NP C+
Sbjct: 289 GQGGYYYKEHLPKLGEHAELQRQESADGHTFQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICR-IGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN----------TFAVGELVRVLDDIESVKRLQAGHGEWTDSMASALGQVGKVLKVYADGDLRVAFGGQTWTFNPACL 454 HSP 3 Score: 73.9442 bits (180), Expect = 6.122e-13 Identity = 41/141 (29.08%), Postives = 73/141 (51.77%), Query Frame = -1
Query: 2298 DVVIAIKDLETIIQMQMAHGGWNDSMNE--CLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASK 2714
D V + +++ + QMQ HGGWN M E C IG + G D V+ + + + L+K + G +V ++ D + ++LQ GHGEW D+M +G+ +V+ + DGD+++ + G T+ NP ++ +
Sbjct: 323 DKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRG-DVRVQYSNNIRWTFHPGALTKVNT-----FAVGELVRVLDDIESVKRLQAGHGEWTDSMASALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSGQ 457
BLAST of SMED30011819 vs. Ensembl Cavefish
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:103034310]) HSP 1 Score: 290.426 bits (742), Expect = 1.137e-83 Identity = 210/667 (31.48%), Postives = 334/667 (50.07%), Query Frame = -1
Query: 123 NILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEA-----------TDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESV--GVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEIL-LDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAF-----KKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGH-VACDVCANNLYECHICRESIENKIR 2054
+ ++GD V ++ +Q+Q+ HGGW +M+ + + G + I GDV+V N + ++P LTK + FA G+ VR+++D VK LQ HGEW SM LG+ GKV ++ADGDLRV Q T NP C+ ++ S+S + S++E L Q+ +L +G VR L + VD + +G L +A ++GH++VVK+L++ +++ AKD+DGD ALH NQ +I LL++KGA+VN NN+ T + +A + G ++ R+L T +AD+++ DS DT LH A+ K + +EIL + D N + FN H AAL+G+ E+ L R ++ D +KED F+A+HL ALNNH D +L+ + D NIRNN QT L +AV Q H+ V +LV A++N D +GDT +H A+ +Q+ ++ ++ S G+LG+ TE + IA +L + GA ++ N KG +P+++ D ST LI + K + + + +R++ PN + + C+IC + + V F C H VAC+ CA+ + +C C+ +I KIR
Sbjct: 256 HTFQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN-----TFAVGELVRVLDDIESVKRLQAGHGEWTDSMASALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSGQPGEVDANLMTAENPSESGSTVISVLEKL----------------------LSQSTEQDNPGRLVIEAAHGSATKVRELVQKHPDKVDIKNQGKTALQVAAHQGHMEVVKVLLQANSSIEAKDEDGDAALHYTAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEILTVVPNIDFTQQNNRGFNLLHHAALKGNKLATEMILSR-ARQLADVKKEDGFSALHLAALNNHRDVAEILVKEGRCDANIRNNRNQTPLQLAVTQGHVALVALLVSEGADVNAEDEEGDTAMHTALSRQQLTNMLSTSEREASALYCRLSSSGLLGN-TE-LNVGAAIACFLAQEGADINYANHKGKSPLDLVTD--------------STTLQLIR---SFSEKHRYVVSSSSSLRRVHTTPNTMTN--------LAMPSAPGPSECLICSELALLVLFSPCQHSVACEECAHRMKKCIKCQVTITKKIR 866 HSP 2 Score: 166.007 bits (419), Expect = 1.247e-41 Identity = 213/864 (24.65%), Postives = 358/864 (41.44%), Query Frame = -1
Query: 117 LVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDI--IGKKVQVIHKFL-DGDVKIKYNGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFG-MEWMINPECIDVKDNILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNGLGLVYNPXXXXXXXXXXXKMFAPGDK--------VRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRN-LCESVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLL-INKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAG-ADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHE------DCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHI---AMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLI--EKEPNPGTKAERLEAPIA-PIRQ------LSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2609
+ QG+ +VRGP + +G+ E G VV+ I+ Q V W++ ++ +G K +V K++ DG Y + A + E S H F+ G++ + VD ++++QE HGGW+ + Y + + I + GD+ V + W +P + K N G+ V + DDI+ K+LQ HG W M+ G+ GK+ + GD++V G +NP L+ + + ++ Q N G V +E G KV+ L +V I NQ T ME + L+ +S +++ G + GNQ E+ +L + K +N + N +C + L IAVN+G DVV++L E A++N +D GDT LH A+ K+ I ++L + D QNN + AA G+ T ++ + + D +ALHLA + + EIL+ G D N N + LA QGH ++ L + EG V+++ E+ A+H AL+ + R A ++ + T L++ + C L + A+IN + G +PL + + ++ I +F + R S + TP+ + L M + G + +C++ L+L + S Q + E+ + K + + I IRQ SP + + LE+L+ R + ME+ TC IC D I + F CGH +C C+ L C ICR++I +I+L+
Sbjct: 158 IFQGV---KVVRGPDWD-----------WGNQDGGEGKIGKVVD-IRGWDQESGRSVASVTWSNGTTNVYRMGHKGKVDLKYVSDGQGGYYYKEHLPKLGEHAELQRQE----SADGHTFQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGAL-TKVNTFAVGELVRVLDDIESVKRLQAGHGEWTDSMASALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSGQPGEVDANLMTAENPSESGSTVISVLEKLLSQSTEQDNPGRLV--IEAAHGSATKVRELVQKHPDKVDIKNQGKTALQVAAHQ------------GHMEVVKVLLQANSSIEAKDEDGDAALHYTAFGNQAEIARLLLS-KGANVNLLNNSMCTA------------LHIAVNKGFTDVVRVLTEHSADVNLQDSYGDTPLHDAIAKDFRSIIEILTVVPNIDFTQQNNRGFNLLHHAALKGNKLATEMILSRARQLADVKKEDGFSALHLAALNNHRDVAEILVKEGRCDANIRNNRNQTPLQLAVTQGHVALVALLVS-EGAD-VNAEDEEGDTAMH-TALSRQQLTNMLSTSEREASALYCRLSSSGLLGNTELNVGAA---IAC--FLAQEGADINYANHKGKSPLDLVTDSTTLQLIRSFSEKHRYVVSSSSS-----LRRVHTTPNTM--------TNLAMPSAPGPSECLICSE-------LALLVLFSPCQHSVACEECAHRMKKCIKCQVTITKKIRQDQTEVECSPNSDNSEQHNLLEQLQSRYRQMEERITCPICIDNQIKLVFQCGHASCIDCSAALKTCPICRQTIRERIQLF 946 HSP 3 Score: 73.9442 bits (180), Expect = 5.829e-13 Identity = 41/141 (29.08%), Postives = 73/141 (51.77%), Query Frame = -1
Query: 2298 DVVIAIKDLETIIQMQMAHGGWNDSMNE--CLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASK 2714
D V + +++ + QMQ HGGWN M E C IG + G D V+ + + + L+K + G +V ++ D + ++LQ GHGEW D+M +G+ +V+ + DGD+++ + G T+ NP ++ +
Sbjct: 262 DKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRG-DVRVQYSNNIRWTFHPGALTKVNT-----FAVGELVRVLDDIESVKRLQAGHGEWTDSMASALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSGQ 396
BLAST of SMED30011819 vs. Ensembl Cavefish
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:352910]) HSP 1 Score: 279.256 bits (713), Expect = 3.006e-79 Identity = 202/671 (30.10%), Postives = 341/671 (50.82%), Query Frame = -1
Query: 120 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXXXXXXXXXXX-----------KMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCES--VGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFL-KREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMG--------ILGDMTEGSD--TPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2048
L+ GD V + D++ + LQ HGGW M G + I D+ V +G +NP +LTK++ + F GD V++ D ++KLLQ+ HGEW +M LGK G+V+ +++D DL+V++ + T NP V + + + E LS+L+ + + D EE++K A NG+L V ++ + V V+ + G + A GH+DV+KLL++ +L A+DKDGD A+H A ++ + ++L GAD+NA+N + TP+ +A + G +++ + L S+ DS+ DT LH A+ K R + + +LL+AGAD+ N FN H AAL+G+ + + L K IVD +K+D + A+HL ALNNH + +L+ A ++I+N QTALH+AV ++H V++LV A+++++D DGDTPLH A++ + +++ M + + I+G T+G++ + IA +L +GA L ++NKKG +P+++C D L + KE G R P++ S +E LEE C++C D + FG CGH+A C +C+ + +C IC++ ++++ ++
Sbjct: 246 LQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVARSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTN--ASGERLSQLLKKLFETQESGDI------------NEELVK---AAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHNVDLEAEDKDGDRAVHHAAFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGSASLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAK-----------CHKEKTSGQVGSR-------------SPSLNSNNETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKDQVQSRTKI 861 HSP 2 Score: 80.8777 bits (198), Expect = 4.812e-15 Identity = 64/193 (33.16%), Postives = 94/193 (48.70%), Query Frame = -1
Query: 2193 GLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDA--IVRGPSGNVYRLNSELLSKYGSDTSQESISGY-----------VVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENH 2732
GL+ GD V I + DLE + +Q HGGW D M E L V GI D +V+ PSGN + N +L+K S E +G +V++ D + + LQ GHGEW + M +GK +V + D D+K++ G+++ NP A+ P S V + GER S++ +KKL E
Sbjct: 245 GLQIGDLVNIDL-DLEIVQSLQHGHGGWTDGMFETLTTT-GTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVARSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKV--APAGSAVTNAS--GERLSQL-----LKKLFETQ 426 HSP 3 Score: 66.6254 bits (161), Expect = 9.500e-11 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = -1
Query: 117 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 299
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 946 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1006 HSP 4 Score: 66.2402 bits (160), Expect = 1.504e-10 Identity = 63/242 (26.03%), Postives = 107/242 (44.21%), Query Frame = -1
Query: 1893 GNVYRLNSELLSKYGSDTSQESIS-GYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKY-NGSTFVINPKAIASKNEKPPVSEVC-------HKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECIDVKDNILRKGDTVI------LSDDIQKTKQLQQNHGGWVSRMSVFA---GKCGKIEAILPTGDVKVN 2564
G YR + +L + + + + G +V + D + + LQ GHG W D M + + V D D+ ++Y +G+ + NP A+ +K EV +F +G+ ++D +K LQ HG W++ ++ GK + +I+ D D+ V G W NP + + G V LS ++K + Q++ G ++ V A G K+E IL DV VN
Sbjct: 224 GTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNP-AVLTKANVARSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV---TKVAPAGSAVTNASGERLSQLLKKLFETQES--GDINEELVKAAANGDLAKVEDILKRPDVDVN 459
BLAST of SMED30011819 vs. Ensembl Cavefish
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:352910]) HSP 1 Score: 278.1 bits (710), Expect = 8.532e-79 Identity = 202/671 (30.10%), Postives = 341/671 (50.82%), Query Frame = -1
Query: 120 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXXXXXXXXXXX-----------KMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCES--VGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFL-KREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMG--------ILGDMTEGSD--TPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2048
L+ GD V + D++ + LQ HGGW M G + I D+ V +G +NP +LTK++ + F GD V++ D ++KLLQ+ HGEW +M LGK G+V+ +++D DL+V++ + T NP V + + + E LS+L+ + + D EE++K A NG+L V ++ + V V+ + G + A GH+DV+KLL++ +L A+DKDGD A+H A ++ + ++L GAD+NA+N + TP+ +A + G +++ + L S+ DS+ DT LH A+ K R + + +LL+AGAD+ N FN H AAL+G+ + + L K IVD +K+D + A+HL ALNNH + +L+ A ++I+N QTALH+AV ++H V++LV A+++++D DGDTPLH A++ + +++ M + + I+G T+G++ + IA +L +GA L ++NKKG +P+++C D L + KE G R P++ S +E LEE C++C D + FG CGH+A C +C+ + +C IC++ ++++ ++
Sbjct: 243 LQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVARSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTN--ASGERLSQLLKKLFETQESGDI------------NEELVK---AAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHNVDLEAEDKDGDRAVHHAAFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGSASLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAK-----------CHKEKTSGQVGSR-------------SPSLNSNNETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKDQVQSRTKI 858 HSP 2 Score: 80.4925 bits (197), Expect = 5.385e-15 Identity = 64/193 (33.16%), Postives = 94/193 (48.70%), Query Frame = -1
Query: 2193 GLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDA--IVRGPSGNVYRLNSELLSKYGSDTSQESISGY-----------VVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENH 2732
GL+ GD V I + DLE + +Q HGGW D M E L V GI D +V+ PSGN + N +L+K S E +G +V++ D + + LQ GHGEW + M +GK +V + D D+K++ G+++ NP A+ P S V + GER S++ +KKL E
Sbjct: 242 GLQIGDLVNIDL-DLEIVQSLQHGHGGWTDGMFETLT-TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVARSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKV--APAGSAVTNAS--GERLSQL-----LKKLFETQ 423 HSP 3 Score: 67.0106 bits (162), Expect = 9.018e-11 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = -1
Query: 117 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 299
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 967 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1027 HSP 4 Score: 65.855 bits (159), Expect = 1.827e-10 Identity = 63/242 (26.03%), Postives = 107/242 (44.21%), Query Frame = -1
Query: 1893 GNVYRLNSELLSKYGSDTSQESIS-GYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKY-NGSTFVINPKAIASKNEKPPVSEVC-------HKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECIDVKDNILRKGDTVI------LSDDIQKTKQLQQNHGGWVSRMSVFA---GKCGKIEAILPTGDVKVN 2564
G YR + +L + + + + G +V + D + + LQ GHG W D M + + V D D+ ++Y +G+ + NP A+ +K EV +F +G+ ++D +K LQ HG W++ ++ GK + +I+ D D+ V G W NP + + G V LS ++K + Q++ G ++ V A G K+E IL DV VN
Sbjct: 221 GTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNP-AVLTKANVARSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV---TKVAPAGSAVTNASGERLSQLLKKLFETQES--GDINEELVKAAANGDLAKVEDILKRPDVDVN 456
BLAST of SMED30011819 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008505.1 (pep scaffold:Pmarinus_7.0:GL477211:28706:49738:-1 gene:ENSPMAG00000007672.1 transcript:ENSPMAT00000008505.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 173.711 bits (439), Expect = 3.876e-45 Identity = 154/564 (27.30%), Postives = 250/564 (44.33%), Query Frame = -1
Query: 477 GDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDV-KVNVNGLGLVYNPXXXXXXXXXXXKMFAPGDK-----VRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDS--------DSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESV--GVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGA-DINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMK-----------------VKQIEAFKKMGR--------MFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPME 2039
GDTV D Q LQQ+HGG+ M++ A + GK+ ++ GDV V NG +YNP L K + + + LI S + L+ + W+ ++ LG+ G+V + GDLRV + NP CV D S DS DI L +L N+++ L G + NV+ L + ++A+ +G L +A ++GHIDV + L++ A+ D G+TALH A N+ + LL++ A VN++N+ T + LAA G E+ R L D++ DS ++ +H A+ ++ +E L D+ N+ F LAA +G ++ L + K+VD QK D FA +HL A N D + +L+ ++ +N R QTAL +A + H++ V +LV ++++ D G+T LH+ +K + QI ++ R +FG GS+ + +A +L+K GA L N +G P +
Sbjct: 242 GDTVRCGVDGQTLMVLQQDHGGFSVAMALIAARPGKVAGLIDNGDVLVVYSNGGKYIYNPKCLIKVKMAQCISHLQLRQSFWIVIALIFSPSCIALINDDSINWMKMVKPTLGRVGRVVKVLPSGDLRVALGGHIWNFNPACVSTADPKSLDLAETSEDSAADILKERVILVWDTDL---------------------NKKQ---LISEAAQGNVGNVQKLVQLFPGQINAQQEGRTALHVAAHQGHIDVARALLKAGASTEIGDDKGNTALHYAAHGNEAAMAGLLVSHRARVNSRNHAGRTALHLAARKGYGEVARTLC-EHACDVNTQDSTKNSPIHDAIAENYEEVIEALTSVKTLDLERQNESGFTPLQLAAFKGSKLAVQRILAKS-PKLVDEQKSDGFAPLHLAAYNGRLDVVHILIKQGRSAVNARTCGGQTALMLAAGRGHVDVVFLLVREACKLDVEDEGGNTALHLVLKEADLFSTDCHLLQDDSTIVQIHYATRVLRINRLRDSGLFGAGSIY-----------AGEALACFLVKEGANLRQTNARGKAPFD 768 HSP 2 Score: 78.1814 bits (191), Expect = 5.105e-15 Identity = 119/598 (19.90%), Postives = 218/598 (36.45%), Query Frame = -1
Query: 1023 GLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGY----VVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKY-NGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECIDVKD---------------NILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNGLGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQN------VTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANL-NAKDKDGDTALHLAVPKNQIKICDLLINKG-ADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILL 2732
G + G +V+ IK ++ Q A W +N +G + D + P+ + + LS G +++ S G+ V D + LQ HG ++ M I + +V +GDV + Y NG ++ NPK + +S + + + I + + + ++ W ++ G+ +V++ GD+ V+ G W NP C+ D +IL++ ++ D+ K + + + G V G K+ + P G + G ++ + G + +DK L HG + + + +V S G + + + TL + DS I + N EEV++ +VK +L G PL +A +G V+ ++ K L + + DG LHLA ++ + +LI +G + VNA+ T +MLAA G V++ LL L + D +TALHL ++++ + S + L
Sbjct: 163 GGEGGKGLVLTIKGWDSTSQRNAAQVRWRGEINSYRLGASGKM-----DLQLASPTSSGRHYYPDHLSCLGDSSNRTSTMGFCLGDTVRCGVDGQTLMVLQQDHGGFSVAMALIAARPGKVAGLIDNGDVLVVYSNGGKYIYNPKCLIKVKMAQCISHLQLRQSFWIVIALIFSPSCIALINDDSINWMKMVKPTLGRVGRVVKVLPSGDLRVALGGHIWNFNPACVSTADPKSLDLAETSEDSAADILKERVILVWDTDLNKKQLISEAAQGNV-------GNVQKLVQLFP-GQINAQQEGRTALH-VAAHQGHIDVARALLKAGASTEIGDDKGNTALHYAAHGNEAAMAGLLVSHRARVNSRNHAGRTALHLAARKGYGEVARTLCEHACDVNTQDSTKNSPIHDAIAE----------------------------NYEEVIEALTSVKTLDLERQNE-----------SGFTPLQLAAFKGSKLAVQRILAKSPKLVDEQKSDGFAPLHLAAYNGRLDVVHILIKQGRSAVNARTCGGQTALMLAAGRGHVDVVFLL-VREACKLDVEDEGGNTALHLVLKEADLFSTDCHL 706
BLAST of SMED30011819 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000093.1 (pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000093.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 122.094 bits (305), Expect = 1.322e-28 Identity = 97/339 (28.61%), Postives = 161/339 (47.49%), Query Frame = -1
Query: 468 LFFAVKNGELNNVR---NLCESVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFK---KMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCN 1466
L A G +N NL +V AR GI PL +A G+ ++VKLL+++ A ++ K +DG T LH A K+ ++L+ + A + A+ N ++P+ +A + +LL R A + D T LH+A ++LLD G + NA F H+A + K++EL LK G I + E IH+ + H + +++LL A N N +T LH+A L VK L+++ A + ++ + TPLHIA ++ + E + + G + G + D+A LL++GA + KKG TP+ + +
Sbjct: 216 LHIAAHYGNMNVATLLLNLGAAVDFTAR-NGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLL-LGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLK-HGASI-HTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIAS 550 HSP 2 Score: 119.398 bits (298), Expect = 1.005e-27 Identity = 75/244 (30.74%), Postives = 125/244 (51.23%), Query Frame = -1
Query: 654 GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMK 1385
G PL A EGH DV LL+E A+++ K G T LH+A ++++ LL+ K A + + N +TP+ +A ++ LL + A + T LH+A +K+++ ++LL+ GA+ N L ++ HLAA +GHA ++ L L R + + A+HL A + + +L +KAD+N + T LH+A ++ V L+ N A +N + +G TPLH A +
Sbjct: 509 GSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVA-LLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHAS--ISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQ 749 HSP 3 Score: 112.849 bits (281), Expect = 1.004e-25 Identity = 105/385 (27.27%), Postives = 164/385 (42.60%), Query Frame = -1
Query: 468 GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDT-----------------------------ALHLAVRKSRVNSVEILL--DAGADINA---LNKKLFNGF------------------------------------HLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAI---------YLLKHGAGLDMKNKKGLTPMEMCN 1385
G+ L +A EGH+DVV L+++ A ++ K G+TALH+A Q I +L+ GA++NAQ+ N TP+ +AA +EI + L A+ S+ D T ALH+A RK N+ +LL + AD+ + LN+ +GF H+AA +G+ +++L L R G KI D++ D +H A + HE + +LL A I + + LH+A +HL CV++L+ A ++ D TPLH+A K + G + + G TP IA LLKHGA + + GLTP+ + +
Sbjct: 43 GLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFL-LDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDR-GAKI-DTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNG------FTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVAS 418 HSP 4 Score: 112.079 bits (279), Expect = 1.669e-25 Identity = 92/334 (27.54%), Postives = 155/334 (46.41%), Query Frame = -1
Query: 414 LKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFK---KMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMC--NDDLLKARILS---LDIPNSTK 1391
L G PL IA + I V++LL++ A+++ + G T +H+A + + LL+ GA NA N TP+ +A+ +G +E+ + L S A + + ++ T LH+A R + V++LL G+D NA + A+ +GH + L L+ G I K+ F +H+ + + +LL A + + T LH+A H +L++ A + +G TPLHIA K Q+E K ++G M + G+ + D+ LL A + + K GLT M + D + A IL+ D+ +TK
Sbjct: 375 LNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQS-GARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLE-NGADISPVTKKG-FTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTK 705 HSP 5 Score: 111.694 bits (278), Expect = 2.797e-25 Identity = 100/394 (25.38%), Postives = 173/394 (43.91%), Query Frame = -1
Query: 408 VMKLFFAVKNGELNNVRNLCE-SVGVDARLK-GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLK--------------------------------REGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCND-DLLKARILSLD---IPNSTKQD 1475
+ L A K G N V+ L + +D + + G+ PL A GH V+++L+E+ A + AK K+G + LH+A + LL+ + A V+ + +TP+ +AA G + +LL R + + + T LH+A +K+R+ +E+LL GA I+ + H+A+ GH +++L L+ + G ++ +ED+ +H+ + + +++LL +D N T L+ A + H + +L+EN A+I+ G TPLHIA K ++E +A LL+ A D K K GLTP+ + D K +L LD P+ST ++
Sbjct: 246 ITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLL-LDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQ-TPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLE------------------------------VATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKN 607 HSP 6 Score: 80.4925 bits (197), Expect = 1.256e-15 Identity = 48/139 (34.53%), Postives = 79/139 (56.83%), Query Frame = -1
Query: 654 VRKSRVNSVEILLD---AGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMK 1061
+R +R ++E L+D G DIN N+ N HLA+ +GH ++ LKRE +D+ + A+H+ AL D +RVL+ + A+IN ++ T L++A + HL VK L++N A ++ DG TPL +A++
Sbjct: 15 LRAARNGNLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAE--IDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQ 151 HSP 7 Score: 70.0922 bits (170), Expect = 1.650e-12 Identity = 48/161 (29.81%), Postives = 84/161 (52.17%), Query Frame = -1
Query: 993 LFFAVKNGELNNVRNLCESVGVDARL---KGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALN 1466
L A K +L + L E +G + + +G+ PL +A EGH D+V LL+ + A+++ K G TA+HLA ++++ + ++L ADVN TP+ +A+ G++++ L +R A ++ + T LH A ++ + V LL A N L
Sbjct: 612 LHIAAKKNQLEVGKVLLE-LGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNR-ALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEASPNELT 770 HSP 8 Score: 56.225 bits (134), Expect = 3.528e-8 Identity = 30/91 (32.97%), Postives = 51/91 (56.04%), Query Frame = -1
Query: 630 VDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFK 902
+++ ++ A+HL + H D + LL +A+I+ + TALHIA L + V++LVE A IN + +G TPL++A + +E K
Sbjct: 36 INTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVK 126
BLAST of SMED30011819 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000095.1 (pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000095.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 121.709 bits (304), Expect = 1.574e-28 Identity = 97/339 (28.61%), Postives = 161/339 (47.49%), Query Frame = -1
Query: 468 LFFAVKNGELNNVR---NLCESVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFK---KMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCN 1466
L A G +N NL +V AR GI PL +A G+ ++VKLL+++ A ++ K +DG T LH A K+ ++L+ + A + A+ N ++P+ +A + +LL R A + D T LH+A ++LLD G + NA F H+A + K++EL LK G I + E IH+ + H + +++LL A N N +T LH+A L VK L+++ A + ++ + TPLHIA ++ + E + + G + G + D+A LL++GA + KKG TP+ + +
Sbjct: 209 LHIAAHYGNMNVATLLLNLGAAVDFTAR-NGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLL-LGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLK-HGASI-HTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIAS 543 HSP 2 Score: 117.087 bits (292), Expect = 4.682e-27 Identity = 90/308 (29.22%), Postives = 147/308 (47.73%), Query Frame = -1
Query: 654 LFFAVKNGELNNVRNLCES---VGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEI-TRLLATSRNAD---------------------------LSIGDSDNDTA------LHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMK 1466
L A + G+L V+ L +S VGV AR + PL IA G ++V+LL+++ ++ NA G T L+ A + + LL+ GAD++ TP+ +A+ G +E+ T LL +AD L G S + TA LH+A +K+++ ++LL+ GA+ N L ++ HLAA +GHA ++ L L R + + A+HL A + + +L +KAD+N + T LH+A ++ V L+ N A +N + +G TPLH A +
Sbjct: 440 LHMASRAGQLEVVKTLLQSGARVGVKAR-EDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHAS--ISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQ 744 HSP 3 Score: 114.775 bits (286), Expect = 2.730e-26 Identity = 99/375 (26.40%), Postives = 155/375 (41.33%), Query Frame = -1
Query: 468 GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDT-----------------------------ALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILEL---------FLKREGVKI----------------------VDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAI---------YLLKHGAGLDMKNKKGLTPMEMCN 1385
G+ L +A EGH+DVV L+++ A ++ K G+TALH+A Q I +L+ GA++NAQ+ N TP+ +AA +EI + L A+ S+ D T ALH+A RK N+ +LL + + +K F H+AA G+ + L F R G+ +D++ D +H A + HE + +LL A I + + LH+A +HL CV++L+ A ++ D TPLH+A K + G + + G TP IA LLKHGA + + GLTP+ + +
Sbjct: 44 GLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFL-LDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNG------FTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVAS 411 HSP 4 Score: 109.768 bits (273), Expect = 9.317e-25 Identity = 100/371 (26.95%), Postives = 163/371 (43.94%), Query Frame = -1
Query: 414 DARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLL---ATSRNADLSIGDS-----------------------------DNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLL--AYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFK---KMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMC--NDDLLKARILS---LDIPNSTK 1400
D L + PL +A + GH V KLL+++ NA+ +G T LH+A KN+IK+ +LL+ GA ++ + +TPI +A+ G + + +LL S NA G++ ++ T LH+A R + V++LL G+D NA + A+ +GH + L L+ G I K+ F +H+ + + +LL AD + T LH+A H +L++ A + +G TPLHIA K Q+E K ++G M + G+ + D+ LL A + + K GLT M + D + A IL+ D+ +TK
Sbjct: 332 DVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLE-NGADISPVTKKG-FTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTK 700 HSP 5 Score: 106.301 bits (264), Expect = 1.205e-23 Identity = 67/221 (30.32%), Postives = 115/221 (52.04%), Query Frame = -1
Query: 723 GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKIL 1385
G+ PL +A + H V LL++K A+ ++ K+G T LH+A KNQ+++ +L+ GA+ N + P+ LAA G ++ LL R+A +S+ TA+HLA ++ +VN EIL AD+N K + H+A+ G+ K++ L+ +V+++ + + +H A H + LL ++A N TAL IA +++ V L
Sbjct: 570 GLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALL-LGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRA--LVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEASPNELTANGNTALAIARRLGYISVVDTL 787 HSP 6 Score: 104.375 bits (259), Expect = 4.273e-23 Identity = 85/347 (24.50%), Postives = 156/347 (44.96%), Query Frame = -1
Query: 471 GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREG-----------------VKIVDSQK----------------EDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAI---------YLLKHGAGLDMKNKKGLTPMEMC 1385
G+ P+ +A GH++VV+LL++ A+ NA + G+T LH+A Q+++ L+ GA V + TP+ +A+ G EI +LL R +D + + T L+ A R+ + +LL+ GADI+ + KK F H+A+ G ++ L L++ D QK ++ + +H+ A N + +VLL A+ N+ LH+A + H + V +L+ +A I+L G T +H+A + ++ + + + + T+ TP +A +LL++ A ++ K + G TP+
Sbjct: 403 GLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLL-LQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKH------KADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQA 742 HSP 7 Score: 83.5741 bits (205), Expect = 1.041e-16 Identity = 61/201 (30.35%), Postives = 102/201 (50.75%), Query Frame = -1
Query: 471 VRKSRVNSVEILLD---AGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSL-MGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMC 1061
+R +R ++E L+D G DIN N+ N HLA+ +GH ++ LKRE +D+ + A+H+ AL D +RVL+ + A+IN ++ T L++A + HL VK L++N A ++ DG TPL +A++ + + G + + L DT + A LL+H D+ +K G TP+ +
Sbjct: 16 LRAARNGNLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAE--IDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKDDT--NAAALLLQHEHNADVTSKSGFTPLHIA 212 HSP 8 Score: 70.0922 bits (170), Expect = 1.543e-12 Identity = 48/161 (29.81%), Postives = 84/161 (52.17%), Query Frame = -1
Query: 993 LFFAVKNGELNNVRNLCESVGVDARL---KGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALN 1466
L A K +L + L E +G + + +G+ PL +A EGH D+V LL+ + A+++ K G TA+HLA ++++ + ++L ADVN TP+ +A+ G++++ L +R A ++ + T LH A ++ + V LL A N L
Sbjct: 607 LHIAAKKNQLEVGKVLLE-LGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNR-ALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEASPNELT 765
BLAST of SMED30011819 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007449.1 (pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007449.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 119.398 bits (298), Expect = 1.245e-27 Identity = 79/279 (28.32%), Postives = 139/279 (49.82%), Query Frame = -1
Query: 654 QEEVMKLFFAVKNGELNNVRNLCESVGVD--ARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMK 1484
+EE L A + G+L V+ L + + A G PL +A EGH DV +L+E A+L K G T LH+A ++++ LL+ + A +A N +TP+ +A+ + ++ LL + A + T LH+A +K ++ LL+ GA+ NA+ ++ HL A +GHA + L L R V+ + + +HL A + + +L+ + A +N + T L +A ++ V L+++ A++N + +G TPLH A +
Sbjct: 112 REEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVA-LLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLAR--YADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQ 387 HSP 2 Score: 115.931 bits (289), Expect = 1.439e-26 Identity = 87/338 (25.74%), Postives = 150/338 (44.38%), Query Frame = -1
Query: 477 KGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREG---------------VKIVDSQK----------------EDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAM---KVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPME 1388
G+ P+ ++ G++++V +L++ A+ NA + G+TALH+A Q ++ L+ GA V+A+ + TP+ +AA G +EI +LL A S+ T LH+A R+ + ILL+ GA + KK F H+A+ G ++ L L+R D+QK ++ + +H+ A + + LL Y A+ N LH+ + H + +L+ A++N G +PLH+A KV E K G + +G + + +LL+HGA ++ K K G TP+
Sbjct: 47 SGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLL-LQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLH 383 HSP 3 Score: 105.145 bits (261), Expect = 3.038e-23 Identity = 84/318 (26.42%), Postives = 149/318 (46.86%), Query Frame = -1
Query: 405 LIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKV---KQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDD-------LLKARILSLDIPNSTKQDL 1328
L++K AN N + +G T LH+A KN+I++ +LL+ GA + A + +TP+ ++A G++ I +L A + + +TALH+A R + V LL GA ++A ++ H+AA G +I++L L+ + D+ + + +H+ A HED +LL + A + + T LH+A + +L++ A + +G TPLH+A K G + G +IA LL++GA + ++G+ P+ + + LL AR D+ ++TK L
Sbjct: 1 LLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQG-GASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQH--MASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARY--ADVNHATKSGL 313 HSP 4 Score: 86.2705 bits (212), Expect = 2.041e-17 Identity = 59/188 (31.38%), Postives = 91/188 (48.40%), Query Frame = -1
Query: 921 GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINA------------------------------LNKKLFNGF---HLAALQGHAKILELFLK 1385
G+ PL +A + + V LL+++ A+ +A K+G T LH+A K+Q++I L+ GA+ NA + P+ L A G ++ LL +R AD++ + LHLA ++ +VN EIL+ GA +NA +N K NG+ H AA QGH I+ L LK
Sbjct: 213 GLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLL-LARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLLK 399 HSP 5 Score: 50.447 bits (119), Expect = 2.295e-6 Identity = 30/89 (33.71%), Postives = 49/89 (55.06%), Query Frame = -1
Query: 1140 VDARLK-GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEI 1403
V+A K G PL++A + G+I +V L++ A++N+K K+G T LH A + I +LL+ GA N N T + +A G + +
Sbjct: 338 VNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLLKCGARPNDVTVNGNTALAIARRLGYISV 426
BLAST of SMED30011819 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007446.1 (pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007446.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 119.398 bits (298), Expect = 1.261e-27 Identity = 85/309 (27.51%), Postives = 150/309 (48.54%), Query Frame = -1
Query: 468 RLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCN 1394
R G+ PL A GH VV++L+E+ A + AK K+G + LH+A + ++ +L+ A V+ + +T + +AA G ++L + A+ ++ + T LH+A +K+R+ +E+LL GA I A+ + H++A G+ I+ + L +G ++ A+H+ A + +R LL A ++ + QT LHIA L V++L+++ A + S+G TPLH+A + EG +D+ LL+HGA L + KKG TP+ + +
Sbjct: 3 RQDGLTPLHCAARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVL-LDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILL--QGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAR---------------------------EGH---EDVTAILLEHGASLAVTTKKGFTPLHVAS 278 HSP 2 Score: 119.398 bits (298), Expect = 1.327e-27 Identity = 79/279 (28.32%), Postives = 139/279 (49.82%), Query Frame = -1
Query: 654 QEEVMKLFFAVKNGELNNVRNLCESVGVD--ARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMK 1484
+EE L A + G+L V+ L + + A G PL +A EGH DV +L+E A+L K G T LH+A ++++ LL+ + A +A N +TP+ +A+ + ++ LL + A + T LH+A +K ++ LL+ GA+ NA+ ++ HL A +GHA + L L R V+ + + +HL A + + +L+ + A +N + T L +A ++ V L+++ A++N + +G TPLH A +
Sbjct: 202 REEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVA-LLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLAR--YADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQ 477 HSP 3 Score: 115.931 bits (289), Expect = 1.914e-26 Identity = 87/336 (25.89%), Postives = 150/336 (44.64%), Query Frame = -1
Query: 480 GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREG---------------VKIVDSQK----------------EDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAM---KVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPM 1385
G+ P+ ++ G++++V +L++ A+ NA + G+TALH+A Q ++ L+ GA V+A+ + TP+ +AA G +EI +LL A S+ T LH+A R+ + ILL+ GA + KK F H+A+ G ++ L L+R D+QK ++ + +H+ A + + LL Y A+ N LH+ + H + +L+ A++N G +PLH+A KV E K G + +G + + +LL+HGA ++ K K G TP+
Sbjct: 138 GLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLL-LQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPL 472 HSP 4 Score: 115.546 bits (288), Expect = 2.232e-26 Identity = 91/342 (26.61%), Postives = 160/342 (46.78%), Query Frame = -1
Query: 405 DARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKV---KQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDD-------LLKARILSLDIPNSTKQDL 1400
D L + L +A + GH K+L++K AN N + +G T LH+A KN+I++ +LL+ GA + A + +TP+ ++A G++ I +L A + + +TALH+A R + V LL GA ++A ++ H+AA G +I++L L+ + D+ + + +H+ A HED +LL + A + + T LH+A + +L++ A + +G TPLH+A K G + G +IA LL++GA + ++G+ P+ + + LL AR D+ ++TK L
Sbjct: 67 DITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQG-GASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQH--MASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARY--ADVNHATKSGL 403 HSP 5 Score: 114.005 bits (284), Expect = 6.279e-26 Identity = 92/342 (26.90%), Postives = 160/342 (46.78%), Query Frame = -1
Query: 474 QEEVMKLFFAVKNGELNNVRNLCE-SVGVDARLK-GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDD---IAIYLLKHGAGLDMKNKKGLTPMEM 1484
Q+ + L A ++G V L E + A+ K G+ PL +A H++ V++L++ RA ++ D TALH+A + +L++KGA+ N + N TP+ +A + + LL A + T +H++ +N V ILL GA NA N + H+AA G +++ L R G + VD++ ++ +H+ A + +++LL + A + + T LH+A + H + IL+E+ A + + G TPLH+A K ++E + + F G G + D +A+ LL GA K G TP+ +
Sbjct: 4 QDGLTPLHCAARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELL-LKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLL-RNGAQ-VDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHI 342 HSP 6 Score: 96.2857 bits (238), Expect = 1.773e-20 Identity = 69/224 (30.80%), Postives = 117/224 (52.23%), Query Frame = -1
Query: 723 GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLL---AYKADINIRNNILQTALHIAVLQKHLNCVKIL 1385
G+ PL +A + + V LL+++ A+ +A K+G T LH+A K+Q++I L+ GA+ NA + P+ L A G ++ LL +R AD++ + LHLA ++ +VN EIL+ GA +NA K + +A G+ K++ FL + G V+S+ ++ + +H A H + +LL A D+ + N TAL IA +++ V L
Sbjct: 303 GLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLL-LARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVN-FLLQHGAD-VNSKTKNGYTPLHQAAQQGHTHIVNLLLKCGARPNDVTVNGN---TALAIARRLGYISVVDTL 520 HSP 7 Score: 80.8777 bits (198), Expect = 1.012e-15 Identity = 46/156 (29.49%), Postives = 73/156 (46.79%), Query Frame = -1
Query: 921 KGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLK 1388
+G+ PL + EGH DV LL+ + A++N K G + LHLA ++++ + ++L+ GA VNA TP+++A G++++ V LL GAD+N+ K + H AA QGH I+ L LK
Sbjct: 368 QGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKM----------------------------------VNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLLK 489 HSP 8 Score: 50.0618 bits (118), Expect = 2.784e-6 Identity = 40/153 (26.14%), Postives = 66/153 (43.14%), Query Frame = -1
Query: 474 SQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKM---GRMFGMGSLMGILGDMTEGSDTPDDIAI---------YLLKHGAGLDMKNKKGLTPMEM 896
+ ++D +H A + HE + +LL A I + + LH+A H+ CV++L++ A ++ D T LH+A K + G + +L G TP IA LLK+GA ++ + GLTPM +
Sbjct: 1 AHRQDGLTPLHCAARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGF---------TPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHV 144
BLAST of SMED30011819 vs. Ensembl Yeast
Match: AKR2 (Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication [Source:SGD;Acc:S000005560]) HSP 1 Score: 70.0922 bits (170), Expect = 1.094e-12 Identity = 61/215 (28.37%), Postives = 100/215 (46.51%), Query Frame = -1
Query: 876 NQEEVMKLFF-AVKNGELNNVRNLCESVGVDA---RLKGIYPLMIAVNEGHIDVVKLLIEKRANLN-AKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSR-----NADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKIL-ELFLKREGVKIVDSQKEDKF 1487
N E V+ +F A K+G+L V+++ ES VD R+ + L A V K L+ + AN N A G TALH A I I DLL+ GAD ++ + + + S ++ + + N D+ D++N T L A + +VE+LL G+ + + + FN H A + G +++ +L L ++QK+D F
Sbjct: 46 NTEYVVDIFIEAAKDGDLKVVKDVVESGAVDINNDRIDELSGLHWACINNRFSVAKFLLLRGANPNQAAGPGGATALHWAARYGNIYIVDLLLKHGADPTLKDEQGLNIMHFSVYSSNILLVVYVLYFVVNNNDNVDIDSKDNNNRTPLLWAAYQGDFLTVELLLKFGSTVAWTDNRGFNALHCALVGGDQRVICDLILSGANFYERNNQKQDCF 260
BLAST of SMED30011819 vs. Ensembl Yeast
Match: AVO2 (Component of a complex containing the Tor2p kinase and other proteins; complex may have a role in regulation of cell growth [Source:SGD;Acc:S000004672]) HSP 1 Score: 68.1662 bits (165), Expect = 2.123e-12 Identity = 58/196 (29.59%), Postives = 96/196 (48.98%), Query Frame = -1
Query: 603 ITRLLATSRNADLSIG-DSDND-TALHLAVRKSRVNSVEILLDAGADINALNKKLFNG---FHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEI----NLRDSDGDTPLHIAMKV-------KQIEAFKKMGRMFGMG 1142
+ RLL RN DL DS+N ++LH A R L+ G D + L K F G HLA ++GH + L L L+ + + ++ + E+ A IH+ +N++ CL +L+ AD+ + + T LH+ + ++C+K+L+ N E+ N+RD P+ +A K ++ KK G G G
Sbjct: 21 VKRLL--RRNPDLLTNIDSENGWSSLHYASYHGRYLICVYLIQLGHDKHEL-IKTFKGNTCVHLALMKGHEQTLHLLLQ-QFPRFINHRGENGRAPIHIACMNDYYQCLSLLIGVGADLWVMDTNGDTPLHVCLEYGSISCMKMLL-NEGEVSLDDNVRDKGNWKPIDVAQTFEVGNIYSKVLKEVKKKGPPLGAG 211
BLAST of SMED30011819 vs. Ensembl Yeast
Match: NAS6 (Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, also known as p28, which interacts with the Rb tumor suppressor and CDK4/6 [Source:SGD;Acc:S000003464]) HSP 1 Score: 58.5362 bits (140), Expect = 7.051e-10 Identity = 61/206 (29.61%), Postives = 96/206 (46.60%), Query Frame = -1
Query: 786 YPLMIAVNEGHI-DVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVN---AQNNNKITPIMLAASSGSVEITR-LLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNG--FHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLA-YKADINIRNN 1379
YPL A E V +LL K + L KD+DG LH +V +I L++K +VN +++ TP +A S G++E+ + L DL+ + T LHLAV K + L++ GA + +K FN H AA G K++EL L G V+ Q + + + H D +L+ Y A+ ++ +N
Sbjct: 4 YPLHQACMENEFFKVQELLHSKPSLLLQKDQDGRIPLHWSVSFQAHEITSFLLSKMENVNLDDYPDDSGWTPFHIACSVGNLEVVKSLYDRPLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKDK--FNQIPLHRAASVGSLKLIEL-LCGLGKSAVNWQDKQGWTPLFHALAEGHGDAAVLLVEKYGAEYDLVDN 206 HSP 2 Score: 57.3806 bits (137), Expect = 1.600e-9 Identity = 40/136 (29.41%), Postives = 70/136 (51.47%), Query Frame = -1
Query: 987 GIYPLMIAVNEGHIDVVKLLIEK--RANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLD-AGADINALNKK 1385
G P IA + G+++VVK L ++ + +LN G T LHLAV K ++ LI GA V ++ P+ AAS GS+++ LL + ++ D T L A+ + ++ +L++ GA+ + ++ K
Sbjct: 72 GWTPFHIACSVGNLEVVKSLYDRPLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLCGLGKSAVNWQDKQGWTPLFHALAEGHGDAAVLLVEKYGAEYDLVDNK 207 HSP 3 Score: 50.0618 bits (118), Expect = 4.258e-7 Identity = 37/123 (30.08%), Postives = 59/123 (47.97%), Query Frame = -1
Query: 1035 KGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKG-ADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSI----GDSDNDTALHLAVRKSRVNSV 1388
+G+ L +AV + +V + LIE A++ KDK LH A +K+ +LL G + VN Q+ TP+ A + G + LL A+ + G D AL+ V+K +N+V
Sbjct: 106 QGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLCGLGKSAVNWQDKQGWTPLFHALAEGHGDAAVLLVEKYGAEYDLVDNKGAKAEDVALNEQVKKFFLNNV 228
BLAST of SMED30011819 vs. Ensembl Yeast
Match: AKR1 (Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication; any of several human homologs encoding DHHC-type zinc fingers (ZDHHC) can complement temperature sensitivity of yeast akr1 null mutant [Source:SGD;Acc:S000002672]) HSP 1 Score: 58.9214 bits (141), Expect = 2.872e-9 Identity = 64/228 (28.07%), Postives = 97/228 (42.54%), Query Frame = -1
Query: 717 AVNEGHIDVVKLLIEKRANLNAKDKDGD-----TALHLAVPKNQIKICDLLINKGADVNAQNNN-KITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILL-----DAGADINALNKKLFNGFHLAALQGHA-KILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVE 1364
A G + VK +I + L + DGD T LH A N++ + D L+++GADVNA+ TP+ AA G V I L AD ++ D LHL+V S + V +L DI+ + K AA QG + + EL +KI D++ F +H + L+ L+ AD + + + IA + +N V L E
Sbjct: 80 ACQRGDLATVKEMIHGK--LLEVNNDGDSTEHITGLHWASINNRLSVVDFLVSQGADVNARAGALHATPLHWAARYGYVYIVDFLL-KHGADPTMTDDQGFNLLHLSVNSSNIMLVLYVLFNVVSKGLLDIDCRDPKGRTSLLWAAYQGDSLTVAELLKFGASIKIADTE---GFTPLHWGTVKGQPHVLKYLIQDGADFFQKTDTGKDCFAIA---QEMNTVYSLRE 298
BLAST of SMED30011819 vs. Ensembl Yeast
Match: HOS4 (Subunit of the Set3 complex; complex is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate [Source:SGD;Acc:S000001374]) HSP 1 Score: 56.225 bits (134), Expect = 1.915e-8 Identity = 37/97 (38.14%), Postives = 59/97 (60.82%), Query Frame = -1
Query: 1128 KGIYP-------LMIAVNEGHIDVVKLLIEKRA-NLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKI--TPIMLAASSGSVEITRLL 1388
KGIY L IA ++G DVVK +IE+ ++N +D G+TALH A + I+I +LLI GADVN ++ TP++ A+++G +++ + L
Sbjct: 322 KGIYRDSGGRTRLQIACDKGKYDVVKKMIEEGGYDINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLIDASANGHLDVVKYL 418 HSP 2 Score: 54.6842 bits (130), Expect = 6.847e-8 Identity = 41/112 (36.61%), Postives = 57/112 (50.89%), Query Frame = -1
Query: 477 DINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSD--GDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPME 806
DIN ++N TALH A LQ H+ V++L+EN A++N++ + GDTPL I+A S G L D+ YLLK+GA ++N KGLT E
Sbjct: 356 DINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPL--------IDA-----------SANGHL-----------DVVKYLLKNGADPTIRNAKGLTAFE 437
BLAST of SMED30011819 vs. Ensembl Nematostella
Match: EDO39113 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SB41]) HSP 1 Score: 87.0409 bits (214), Expect = 1.484e-19 Identity = 65/187 (34.76%), Postives = 97/187 (51.87%), Query Frame = -1
Query: 819 AVNEGHIDVV-KLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNN---NKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILL-DAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLL 1364
A +G D V KLL NLN D DG + LH A N + I +L++ GADV+ + + +I PI+ AA ++E R+L + AD++I + TALH A R+ VE+LL D D+N +++ H+AA+ G I++ L G I ++ D F A HL A E+ L+ LL
Sbjct: 2 ACRDGRKDCVRKLLRGNLTNLNTLDADGFSPLHYATTTNHVDIVQMLLDAGADVDVRGSPSIQRIPPIICAAKFNAIESCRVLLRA-GADVNITNCYGQTALHHATRRGHSKMVELLLRDGQMDVNVVDRDNTTSLHVAAMIGSNPIIKTLLF-CGANIT-AKDNDGFTAFHLAAREGEEEVLQTLL 185 HSP 2 Score: 76.6406 bits (187), Expect = 5.520e-16 Identity = 49/161 (30.43%), Postives = 81/161 (50.31%), Query Frame = -1
Query: 921 GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALH------LAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLK 1385
G PL A H+D+V++L++ A+++ + G ++ A N I+ C +L+ GADVN N T + A G ++ LL D+++ D DN T+LH+A ++ LL GA+I A + F FHLAA +G ++L+ L+
Sbjct: 29 GFSPLHYATTTNHVDIVQMLLDAGADVDVR---GSPSIQRIPPIICAAKFNAIESCRVLLRAGADVNITNCYGQTALHHATRRGHSKMVELLLRDGQMDVNVVDRDNTTSLHVAAMIGSNPIIKTLLFCGANITAKDNDGFTAFHLAAREGEEEVLQTLLR 186 HSP 3 Score: 56.6102 bits (135), Expect = 4.601e-9 Identity = 46/158 (29.11%), Postives = 79/158 (50.00%), Query Frame = -1
Query: 1014 LFFAVKNGELNNVRNLCESVGVDARLKG------IYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGA-DVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAG 1466
L +A ++ V+ L ++ G D ++G I P++ A I+ ++L+ A++N + G TALH A + K+ +LL+ G DVN + + T + +AA GS I + L A+++ D+D TA HLA R+ ++ LL G
Sbjct: 33 LHYATTTNHVDIVQMLLDA-GADVDVRGSPSIQRIPPIICAAKFNAIESCRVLLRAGADVNITNCYGQTALHHATRRGHSKMVELLLRDGQMDVNVVDRDNTTSLHVAAMIGSNPIIKTLLFC-GANITAKDNDGFTAFHLAAREGEEEVLQTLLRTG 188
BLAST of SMED30011819 vs. Ensembl Nematostella
Match: EDO49485 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG84]) HSP 1 Score: 86.2705 bits (212), Expect = 7.050e-19 Identity = 71/230 (30.87%), Postives = 112/230 (48.70%), Query Frame = -1
Query: 612 LHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQG-HAKILELFLKREGVKIVDSQKEDK--FAAIHLVALNNHEDCLRVLLAYKA-DINIRN--NILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAM---KVKQIEAFKKMGRMF 1274
LHLA + C L+ G DVNA++NN TP+ AA SG+V LL S+ D + D LH A + V L+ AG+++N + K H AA AK +E L+ G ++ K DK ++A+H + N H+ + +LL A D+ R+ + T LH+A H +++L+++ ++ +RD G TPL +A V+ +E MF
Sbjct: 4 LHLACLGGFTECCKKLLFYGVDVNAKDNNDRTPLHCAACSGNVNCLELL-MSQAGDFNCIDKLGRNPLHYAAGHTNFRCVMSLVTAGSNVNKPDTKACTPLHYAAASDLDAKCVEHLLRNRG----NASKRDKQGYSAVHYASANGHKLAIEMLLDVSANDLLNRSSGSPCVTPLHLAAFHGHKPALEVLIKSFNDLEVRDDRGRTPLDLASFQGHVECVECLLNHNAMF 228 HSP 2 Score: 53.5286 bits (127), Expect = 8.640e-8 Identity = 42/156 (26.92%), Postives = 68/156 (43.59%), Query Frame = -1
Query: 408 IHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDT--PDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQD 869
+HL L +C + LL Y D+N ++N +T LH A ++NC+++L+ + N D G PLH A G + ++ +T GS+ PD A L + A D+ K C + LL+ R N++K+D
Sbjct: 4 LHLACLGGFTECCKKLLFYGVDVNAKDNNDRTPLHCAACSGNVNCLELLMSQAGDFNCIDKLGRNPLHYAA---------------GHTNFRCVMSLVTAGSNVNKPDTKACTPLHYAAASDLDAK--------CVEHLLRNR------GNASKRD 130
BLAST of SMED30011819 vs. Ensembl Nematostella
Match: EDO38702 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC57]) HSP 1 Score: 87.0409 bits (214), Expect = 8.090e-18 Identity = 70/244 (28.69%), Postives = 115/244 (47.13%), Query Frame = -1
Query: 630 VVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKS-------RVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFK 1340
++ +I RA + +DKD DT L +A KN + L+ GAD+ A++ N + AA+ G +E + +I D D L+ V S R++S ++LLD GA I++ N + H AA G + + LKR+ I++ + +HL AL+ H ++ LL I+ RN L T L A + + IL++ +A ++ D TPLH+A + +E K
Sbjct: 162 IICYIIRFRACIECRDKDKDTPLMIAAEKNHVDTVRTLLQHGADIAAKDINDRNSVFRAAAEGCMETLEAALEHQKPVWTICD---DVCLNGLVGGSNKLILVLRISS-QLLLDNGACIDSKNDEDITPIHYAAKFGRVRTVCALLKRDPA-IINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVK 400 HSP 2 Score: 77.7962 bits (190), Expect = 7.672e-15 Identity = 65/248 (26.21%), Postives = 111/248 (44.76%), Query Frame = -1
Query: 744 PLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIK--------------ICD-----------------------LLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKH 1376
PLMIA + H+D V+ L++ A++ AKD + ++ A + ++ ICD LL++ GA ++++N+ ITPI AA G V L A ++ D ++T LHLA V+ LL G I+A N L+ AA +G ++ + L + VD + K +HL + H + +++LL+ KA+I + ++ + L IA+ H
Sbjct: 183 PLMIAAEKNHVDTVRTLLQHGADIAAKDINDRNSVFRAAAEGCMETLEAALEHQKPVWTICDDVCLNGLVGGSNKLILVLRISSQLLLDNGACIDSKNDEDITPIHYAAKFGRVRTVCALLKRDPAIINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAP--VDPTDKAKTTPLHLASREGHVEMVKLLLSRKANITLTDSAGRNCLDIAIESSH 428 HSP 3 Score: 67.0106 bits (162), Expect = 1.744e-11 Identity = 82/361 (22.71%), Postives = 143/361 (39.61%), Query Frame = -1
Query: 477 TALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIG-----------DSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILEL----------FLKREGVK----------------------IVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKA-----DINIRNNILQTALH--IAVLQKHLN------------CV------------------KILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLM----GILGDMTEGSDTPDDIAIY---------LLKHGAGLDMKNKKGLTPME 1280
T LH+A I + L+ GAD+ + + ++TP+ A G++ + +LL + A+L G D + TALHLAV + ++ L+ GA++NA+ + FH + I + F K E +K ++ + +DK + + A NH D +R LL + A DIN RN++ + A + L+ L C+ ++L++N A I+ ++ + TP+H A K GR+ + +L+ I+ D S+TP +A LL G +D +N TP++
Sbjct: 6 TPLHVACSHGCIPVAKCLVEAGADLRSLDEEQMTPLHFACMEGNLGVAKLLFVA--AELRGGWSTVSKMVTDQDREEQTALHLAVEGGHGDLAKLCLEKGANVNAVKESKNKTFHKTIVVARECIFVMIKVSKLKYLSFCKSEKIKREILLYFFDVVIIICYIIRFRACIECRDKDKDTPLMIAAEKNHVDTVRTLLQHGADIAAKDINDRNSVFRAAAEGCMETLEAALEHQKPVWTICDDVCLNGLVGGSNKLILVLRISSQLLLDNGACIDSKNDEDITPIHYA---------AKFGRVRTVCALLKRDPAIINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLD 355 HSP 4 Score: 63.1586 bits (152), Expect = 2.969e-10 Identity = 43/152 (28.29%), Postives = 78/152 (51.32%), Query Frame = -1
Query: 1050 NQEEVMKLFFAVKNGELNNVRNLCESVGVDARL------KGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKS 1487
N E++ + +A K G VR +C + D + PL +A GH VVK L+ + ++A++ T L A + +++ ++L+++ A V+ + K TP+ LA+ G VE+ +LL SR A++++ DS L +A+ S
Sbjct: 280 NDEDITPIHYAAKFGR---VRTVCALLKRDPAIINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLL-LSRKANITLTDSAGRNCLDIAIESS 427
BLAST of SMED30011819 vs. Ensembl Nematostella
Match: EDO43690 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXV8]) HSP 1 Score: 75.8702 bits (185), Expect = 7.329e-15 Identity = 67/239 (28.03%), Postives = 117/239 (48.95%), Query Frame = -1
Query: 735 LMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLA-------------------VRKSRVNSVEILL-DAGADIN------ALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNC 1373
+ AV+ G +D VK L+ K A++N ++ DG TALH A + +L++KGA ++ ++N T + AAS GSVEI + L AD++ +++ + L LA V +R +I+L DA + +N + +K + H+A+ +G+ K++ L L+ GV+I +++ D + +H A +D +L AD N RN ++ L+ L C
Sbjct: 53 FLSAVSSGDVDEVKKLLAKGADINYQNVDGLTALHQACIDENFDLVKVLVDKGASMDVRDNEGWTSLHAAASCGSVEIAKYL-IENGADVAAVNNEGELPLDLAEEEDMEDLLTDEIENQGIDVDDARAVEEKIMLNDAQSWLNKNCINESKDKTEASALHVASSKGYLKVIGLLLQL-GVEI-NAKDADNWTPLHAAAHWGQKDACELLSENGADFNARNKMVGIDHQQNWLKNFLEC 288 HSP 2 Score: 65.4698 bits (158), Expect = 2.082e-11 Identity = 56/211 (26.54%), Postives = 104/211 (49.29%), Query Frame = -1
Query: 636 AVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADIN-IRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEA 1265
AV + L+ KGAD+N QN + +T + A + ++ ++L + A + + D++ T+LH A V + L++ GAD+ A+N + LA + +L ++ +G+ + D++ A + LN+ + L K IN ++ +ALH+A + +L + +L++ EIN +D+D TPLH A Q +A
Sbjct: 56 AVSSGDVDEVKKLLAKGADINYQNVDGLTALHQACIDENFDLVKVL-VDKGASMDVRDNEGWTSLHAAASCGSVEIAKYLIENGADVAAVNNEGELPLDLAEEEDMEDLLTDEIENQGIDVDDAR-----AVEEKIMLNDAQSWLN-----KNCINESKDKTEASALHVASSKGYLKVIGLLLQLGVEINAKDADNWTPLHAAAHWGQKDA 255 HSP 3 Score: 52.7582 bits (125), Expect = 2.984e-7 Identity = 36/126 (28.57%), Postives = 61/126 (48.41%), Query Frame = -1
Query: 462 DCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDD 839
D ++ LLA ADIN +N TALH A + ++ + VK+LV+ A +++RD++G T LH A +E IA YL+++GA + N +G P+++ ++
Sbjct: 63 DEVKKLLAKGADINYQNVDGLTALHQACIDENFDLVKVLVDKGASMDVRDNEGWTSLHAAASCGSVE------------------------------IAKYLIENGADVAAVNNEGELPLDLAEEE 158
BLAST of SMED30011819 vs. Ensembl Nematostella
Match: EDO38725 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC11]) HSP 1 Score: 73.1738 bits (178), Expect = 1.665e-14 Identity = 46/152 (30.26%), Postives = 76/152 (50.00%), Query Frame = -1
Query: 921 PLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLK 1376
PL +A +G ++V +LL+EK A L+ K+ G+T LH+A L++KG ++ ++ + TP + A ++G E LL D + + LHLA R VE L AG N +++ + H+AAL G K++ L+
Sbjct: 39 PLHVACKKGSMEVGRLLVEKGAALDKKNDKGETPLHMAACIGSFDALKFLLSKGVAIDERDFSHRTPFLSAVAAGRTENAMLLLEK--------DENQKSPLHLACAAGRALHVEALTRAGLSTNERDEEGQSALHMAALSGFRKVVTQLLR 182 HSP 2 Score: 65.0846 bits (157), Expect = 1.076e-11 Identity = 62/225 (27.56%), Postives = 100/225 (44.44%), Query Frame = -1
Query: 639 LNAKDKDGDTALHLAVPKNQIKICDLLINKGA--DVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIE 1307
LN D G +ALH A N+++ LI G+ D++ TP+ +A GS+E+ RLL + A L + +T LH+A ++++ LL G I+ + F A G + L L+++ E++ + +HL + L N R+ Q+ALH+A L V L+ A+IN RDS+ TPL A K ++
Sbjct: 1 LNKMDDTGFSALHHATRYNRVEAMAELIRNGSIEDLS-------TPLHVACKKGSMEVGRLL-VEKGAALDKKNDKGETPLHMAACIGSFDALKFLLSKGVAIDERDFSHRTPFLSAVAAGRTENAMLLLEKD---------ENQKSPLHLACAAGRALHVEALTRAGLSTNERDEEGQSALHMAALSGFRKVVTQLLRYGADINARDSNRWTPLMCAAKTGSVK 208 HSP 3 Score: 64.3142 bits (155), Expect = 1.858e-11 Identity = 63/228 (27.63%), Postives = 104/228 (45.61%), Query Frame = -1
Query: 702 GIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEI 1385
G L A ++ + LI N +D T LH+A K +++ LL+ KGA ++ +N+ TP+ +AA GS + + L S+ + D + T AV R + +LL+ N+K + HLA G A +E L R G+ + + E+ +A+H+ AL+ + LL Y ADIN R++ T L A + L+++ A I
Sbjct: 8 GFSALHHATRYNRVEAMAELIR-----NGSIEDLSTPLHVACKKGSMEVGRLLVEKGAALDKKNDKGETPLHMAACIGSFDALKFL-LSKGVAIDERDFSHRTPFLSAVAAGRTENAMLLLEKDE-----NQK--SPLHLACAAGRALHVEA-LTRAGLS-TNERDEEGQSALHMAALSGFRKVVTQLLRYGADINARDSNRWTPLMCAAKTGSVKTCMELLQSGASI 220
BLAST of SMED30011819 vs. Ensembl Medaka
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:101175479]) HSP 1 Score: 308.531 bits (789), Expect = 1.168e-89 Identity = 229/743 (30.82%), Postives = 353/743 (47.51%), Query Frame = -1
Query: 117 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEA-----------TDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESVG--VDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGA-DINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMG------------------SLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEM--------------------------CNDDLLKARILSLDIPNSTKQDLIEKEPNPGTK--------------------------AERLEAPI-------APIRQ------LSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2048
++GD V ++ +Q+Q+ HGGW +M+ + + G + I GDV+V N + ++P LTK + F G+ VR++++ VK LQ HGEW SM LG+ GKV ++ADGDLRV Q T NP C+ A T++ ++S + S++E L Q+ +L +G VR L + VD + +G L +A ++GH++VVK L++ ++ KD+DGDTALH A NQ +I LL++KGA+VN NN+ T + +A + G ++ R+L T +AD+++ DS DT LH A+ K + +E+L+ + D N + FN H AAL+G+ E L R ++VD +K+D F+A+HL ALNNH D VL+ + DIN+RNN QTAL +AV Q H V++LV+ A++N+ D DGDT +H+A+ Q+ A + G G S G+LG+ T IA +L + GA ++ N KG +P+++ C L A + + +T +L+ P PG A R++ I IRQ SP + LE+L+ R + ME+ TC IC D I + F CGH +C C+ L C ICR++I +I+L+
Sbjct: 318 FQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN-----TFGVGELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPVDVDANLMTTENPNESGSTVISVLEKL----------------------LSQSTEQDNPCRLVIEAAHGSAVKVRELVQKHPDKVDIKNQGKTALQVAAHQGHMEVVKALLQANCSVEVKDEDGDTALHYAAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEMLVVVPSIDFTQQNHRGFNLLHHAALKGNKLATEKILAR-ARQLVDVKKDDGFSALHLAALNNHRDVAEVLVKEGRCDINVRNNRNQTALQLAVTQGHTELVQLLVDEGADVNMEDEDGDTAMHVALLRPQL-ANVMLSPAVGTGATEDNSEGCSPTTLHGRLSSSGLLGNGELSVGTA--IACFLAQEGADINYANHKGKSPLDLLADSGALQLIKSFSEKHRLQRLQAITCGQGLSSASLRRVHTTPNTMTNLVLPTP-PGPSECLICSELALLVLFCPCQHSVACEECAHRMKKCIKCQVTITKKIRQDQTEVDCSPGTENSEQHNLLEQLQSRYRQMEERITCPICIDNHIKLVFQCGHASCIECSAALKTCPICRQTIRERIQLF 1027 HSP 2 Score: 88.5817 bits (218), Expect = 2.023e-17 Identity = 58/202 (28.71%), Postives = 97/202 (48.02%), Query Frame = -1
Query: 2025 GPSGNVYRLNSELLSKYGSDTSQESISGYV------VELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKY-NGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPEC-----IDVKDNIL------RKGDTVI 2573
G G Y+ + L ++ QES G+ V+ + + RQ+Q GHG WN M + I + + +H+ D GDV+++Y N + +P A+ N F +GE + V+T+K+LQ HG W+D + G+ ++++++ DGD+ V+ G W NP C +DV N++ G TVI
Sbjct: 288 GQGGFYYKDHLPKLGEHAELQRQESADGHSFQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICR-IGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN----------TFGVGELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPVDVDANLMTTENPNESGSTVI 478 HSP 3 Score: 73.559 bits (179), Expect = 8.164e-13 Identity = 40/141 (28.37%), Postives = 75/141 (53.19%), Query Frame = -1
Query: 2298 DVVIAIKDLETIIQMQMAHGGWNDSMNE--CLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASK 2714
D V + +++ + QMQ HGGWN M E C IG + G D V+ + + + L+K + G +V ++ + + ++LQ GHGEW D+M ++G+ +V+ + DGD+++ + G T+ NP ++++
Sbjct: 322 DKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRG-DVRVQYSNNIRWTFHPGALTKVNTFGV-----GELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQ 456
BLAST of SMED30011819 vs. Ensembl Medaka
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:101175479]) HSP 1 Score: 308.916 bits (790), Expect = 2.058e-89 Identity = 229/743 (30.82%), Postives = 353/743 (47.51%), Query Frame = -1
Query: 117 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEA-----------TDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESVG--VDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGA-DINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMG------------------SLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEM--------------------------CNDDLLKARILSLDIPNSTKQDLIEKEPNPGTK--------------------------AERLEAPI-------APIRQ------LSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2048
++GD V ++ +Q+Q+ HGGW +M+ + + G + I GDV+V N + ++P LTK + F G+ VR++++ VK LQ HGEW SM LG+ GKV ++ADGDLRV Q T NP C+ A T++ ++S + S++E L Q+ +L +G VR L + VD + +G L +A ++GH++VVK L++ ++ KD+DGDTALH A NQ +I LL++KGA+VN NN+ T + +A + G ++ R+L T +AD+++ DS DT LH A+ K + +E+L+ + D N + FN H AAL+G+ E L R ++VD +K+D F+A+HL ALNNH D VL+ + DIN+RNN QTAL +AV Q H V++LV+ A++N+ D DGDT +H+A+ Q+ A + G G S G+LG+ T IA +L + GA ++ N KG +P+++ C L A + + +T +L+ P PG A R++ I IRQ SP + LE+L+ R + ME+ TC IC D I + F CGH +C C+ L C ICR++I +I+L+
Sbjct: 374 FQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN-----TFGVGELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPVDVDANLMTTENPNESGSTVISVLEKL----------------------LSQSTEQDNPCRLVIEAAHGSAVKVRELVQKHPDKVDIKNQGKTALQVAAHQGHMEVVKALLQANCSVEVKDEDGDTALHYAAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEMLVVVPSIDFTQQNHRGFNLLHHAALKGNKLATEKILAR-ARQLVDVKKDDGFSALHLAALNNHRDVAEVLVKEGRCDINVRNNRNQTALQLAVTQGHTELVQLLVDEGADVNMEDEDGDTAMHVALLRPQL-ANVMLSPAVGTGATEDNSEGCSPTTLHGRLSSSGLLGNGELSVGTA--IACFLAQEGADINYANHKGKSPLDLLADSGALQLIKSFSEKHRLQRLQAITCGQGLSSASLRRVHTTPNTMTNLVLPTP-PGPSECLICSELALLVLFCPCQHSVACEECAHRMKKCIKCQVTITKKIRQDQTEVDCSPGTENSEQHNLLEQLQSRYRQMEERITCPICIDNHIKLVFQCGHASCIECSAALKTCPICRQTIRERIQLF 1083 HSP 2 Score: 88.1965 bits (217), Expect = 2.267e-17 Identity = 58/202 (28.71%), Postives = 97/202 (48.02%), Query Frame = -1
Query: 2025 GPSGNVYRLNSELLSKYGSDTSQESISGYV------VELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKY-NGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPEC-----IDVKDNIL------RKGDTVI 2573
G G Y+ + L ++ QES G+ V+ + + RQ+Q GHG WN M + I + + +H+ D GDV+++Y N + +P A+ N F +GE + V+T+K+LQ HG W+D + G+ ++++++ DGD+ V+ G W NP C +DV N++ G TVI
Sbjct: 344 GQGGFYYKDHLPKLGEHAELQRQESADGHSFQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICR-IGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN----------TFGVGELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPVDVDANLMTTENPNESGSTVI 534 HSP 3 Score: 75.485 bits (184), Expect = 1.979e-13 Identity = 39/136 (28.68%), Postives = 69/136 (50.74%), Query Frame = -1
Query: 1863 HKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFG-MEWMINPECIDVKDNILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNGLGLVYNP 2267
H F+ G++ + VD ++++QE HGGW+ + Y + + I + GD+ V + W +P + K N G+ V + D+++ K+LQ HG W M+ G+ GK+ + GD++V G +NP
Sbjct: 372 HSFQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGAL-TKVNTFGVGELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNP 506 HSP 4 Score: 73.559 bits (179), Expect = 8.629e-13 Identity = 40/141 (28.37%), Postives = 75/141 (53.19%), Query Frame = -1
Query: 2298 DVVIAIKDLETIIQMQMAHGGWNDSMNE--CLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASK 2714
D V + +++ + QMQ HGGWN M E C IG + G D V+ + + + L+K + G +V ++ + + ++LQ GHGEW D+M ++G+ +V+ + DGD+++ + G T+ NP ++++
Sbjct: 378 DKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRG-DVRVQYSNNIRWTFHPGALTKVNTFGV-----GELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQ 512
BLAST of SMED30011819 vs. Ensembl Medaka
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:101175479]) HSP 1 Score: 287.345 bits (734), Expect = 1.320e-82 Identity = 218/685 (31.82%), Postives = 339/685 (49.49%), Query Frame = -1
Query: 123 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEA-----------TDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESV--GVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGA-DINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMG------------------SLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEA-------PIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTF-GCGH-VACDVCANNLYECHICRESIENKIR 2048
++GD V ++ +Q+Q+ HGGW +M+ + + G + I GDV+V N + ++P LTK + F G+ VR++++ VK LQ HGEW SM LG+ GKV ++ADGDLRV Q T NP C+ A T++ ++S + S++E L Q+ +L +G VR L + VD + +G L +A ++GH++VVK L++ ++ KD+DGDTALH A NQ +I LL++KGA+VN NN+ T + +A + G ++ R+L T +AD+++ DS DT LH A+ K + +E+L+ + D N + FN H AAL+G+ E L R ++VD +K+D F+A+HL ALNNH D VL+ + DIN+RNN QTAL +AV Q H V++LV+ A++N+ D DGDT +H+A+ Q+ A + G G S G+LG+ T IA +L + GA ++ N KG +P+++ D + L L S K L +RL+A A +R++ PN + + C+IC + + V F C H VAC+ CA+ + +C C+ +I KIR
Sbjct: 249 FQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN-----TFGVGELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPVDVDANLMTTENPNESGSTVISVLEKL----------------------LSQSTEQDNPCRLVIEAAHGSAVKVRELVQKHPDKVDIKNQGKTALQVAAHQGHMEVVKALLQANCSVEVKDEDGDTALHYAAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEMLVVVPSIDFTQQNHRGFNLLHHAALKGNKLATEKILAR-ARQLVDVKKDDGFSALHLAALNNHRDVAEVLVKEGRCDINVRNNRNQTALQLAVTQGHTELVQLLVDEGADVNMEDEDGDTAMHVALLRPQL-ANVMLSPAVGTGATEDNSEGCSPTTLHGRLSSSGLLGNGELSVGTA--IACFLAQEGADINYANHKGKSPLDLLAD----SGALQLIKSFSEKHRL-----------QRLQAITCGQGLSSASLRRVHTTPNTMT--------NLVLPTPPGPSECLICSELALLVLFCPCQHSVACEECAHRMKKCIKCQVTITKKIR 878 HSP 2 Score: 103.605 bits (257), Expect = 4.233e-22 Identity = 158/741 (21.32%), Postives = 277/741 (37.38%), Query Frame = -1
Query: 210 HKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFG-MEWMINPECIDVKDNILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNGLGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSS-------MEFFLGKKGKVKSLFADGDLRVKIMNQNVT-----------------LNPEC-VEATDSDSDS--EPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESVGVDARLKGIY---PLMIAVNEGHIDVVKLL-IEKRANLNAKDKDGDTALHLAVPK-NQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADIN--IRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGIL-------GDMTEGSDTPDDIAIYLLKHGAGLD---MKNKKGL----------TPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTF 2267
H F+ G++ + VD ++++QE HGGW+ + Y + + I + GD+ V + W +P + K N G+ V + D+++ K+LQ HG W M+ G+ GK+ + GD++V G +NP L+ + + + + +L+K + +E G KV+ L +V I NQ T L C VE D D D+ F +++L+ N L AV G + VR L E D L+ Y PL A+ + ++++L + + ++ G LH A K N++ +L V+ + ++ + + LAA + ++ +L D+++ ++ N TAL LAV + V++L+D GAD+N ++ H+A L+ ++ + L + ED N E C L + + + N L IA L + A+IN + G +PL + ++ K + L I + TP+ + +L G + ++ L E C + K + I +QD E + +PGT+ S N+ LE+L+ R + ME+ TC IC D I + F
Sbjct: 247 HSFQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGAL-TKVNTFGVGELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPVDVDANLMTTENPNE-SGSTVISVLEKLLSQSTEQDNPCRLVIEAAHGSAVKVRELVQKHPDKVDIKNQGKTALQVAAHQGHMEVVKALLQANCSVEVKDEDGDTALHYAAFGNQAEIARLL-------------LSKGANVNLLNNSMCTALHIAVNKGFTDVVRVLTEH-SADVNLQDSYGDTPLHDAIAKDFRSIIEMLVVVPSIDFTQQNHRGFNLLHHAALKGNKLATEKILARARQLVDVKKDDGFSALHLAALNNHRDVAEVLVKEGRCDINVRNNRNQTALQLAVTQGHTELVQLLVDEGADVNMEDEDGDTAMHVALLR--PQLANVMLSP---AVGTGATED-----------NSEGCSPTTLHGRLSSSGLLGNGELSVGTAIACF---------LAQEGADINYANHKGKSPLDLLADSGALQLIKSFSEKHRLQRLQAITCGQGLSSASLRRVHTTPNTMTNLVLPTPPGPSECLICSELALLVLFCPCQHSVACEECAHRMKKCIKCQVTITKKIRQDQTEVDCSPGTEN-------------SEQHNL------LEQLQSRYRQMEERITCPICIDNHIKLVF 927 HSP 3 Score: 87.4261 bits (215), Expect = 3.993e-17 Identity = 58/202 (28.71%), Postives = 97/202 (48.02%), Query Frame = -1
Query: 2025 GPSGNVYRLNSELLSKYGSDTSQESISGYV------VELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKY-NGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPEC-----IDVKDNIL------RKGDTVI 2573
G G Y+ + L ++ QES G+ V+ + + RQ+Q GHG WN M + I + + +H+ D GDV+++Y N + +P A+ N F +GE + V+T+K+LQ HG W+D + G+ ++++++ DGD+ V+ G W NP C +DV N++ G TVI
Sbjct: 219 GQGGFYYKDHLPKLGEHAELQRQESADGHSFQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICR-IGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN----------TFGVGELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPVDVDANLMTTENPNESGSTVI 409 HSP 4 Score: 73.1738 bits (178), Expect = 1.066e-12 Identity = 40/141 (28.37%), Postives = 75/141 (53.19%), Query Frame = -1
Query: 2298 DVVIAIKDLETIIQMQMAHGGWNDSMNE--CLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASK 2714
D V + +++ + QMQ HGGWN M E C IG + G D V+ + + + L+K + G +V ++ + + ++LQ GHGEW D+M ++G+ +V+ + DGD+++ + G T+ NP ++++
Sbjct: 253 DKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRG-DVRVQYSNNIRWTFHPGALTKVNTFGV-----GELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQ 387
BLAST of SMED30011819 vs. Ensembl Medaka
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:101175479]) HSP 1 Score: 287.345 bits (734), Expect = 6.926e-82 Identity = 219/685 (31.97%), Postives = 342/685 (49.93%), Query Frame = -1
Query: 123 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEA-----------TDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESVG--VDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGA-DINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMG------------------SLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEA-------PIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTF-GCGH-VACDVCANNLYECHICRESIENKIR 2048
++GD V ++ +Q+Q+ HGGW +M+ + + G + I GDV+V N + ++P LTK + F G+ VR++++ VK LQ HGEW SM LG+ GKV ++ADGDLRV Q T NP C+ A T++ ++S + S++E KL++ Q+ +L +G VR L + VD + +G L +A ++GH++VVK L++ ++ KD+DGDTALH A NQ +I LL++KGA+VN NN+ T + +A + G ++ R+L T +AD+++ DS DT LH A+ K + +E+L+ + D N + FN H AAL+G+ E L R ++VD +K+D F+A+HL ALNNH D VL+ + DIN+RNN QTAL +AV Q H V++LV+ A++N+ D DGDT +H+A+ Q+ A + G G S G+LG+ T IA +L + GA ++ N KG +P+++ D + L L S K L +RL+A A +R++ PN + + C+IC + + V F C H VAC+ CA+ + +C C+ +I KIR
Sbjct: 374 FQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN-----TFGVGELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPVDVDANLMTTENPNESGSTVISVLE---KLLS-------------------QSTEQDNPCRLVIEAAHGSAVKVRELVQKHPDKVDIKNQGKTALQVAAHQGHMEVVKALLQANCSVEVKDEDGDTALHYAAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEMLVVVPSIDFTQQNHRGFNLLHHAALKGNKLATEKILAR-ARQLVDVKKDDGFSALHLAALNNHRDVAEVLVKEGRCDINVRNNRNQTALQLAVTQGHTELVQLLVDEGADVNMEDEDGDTAMHVALLRPQL-ANVMLSPAVGTGATEDNSEGCSPTTLHGRLSSSGLLGNGELSVGTA--IACFLAQEGADINYANHKGKSPLDLLAD----SGALQLIKSFSEKHRL-----------QRLQAITCGQGLSSASLRRVHTTPNTMT--------NLVLPTPPGPSECLICSELALLVLFCPCQHSVACEECAHRMKKCIKCQVTITKKIR 1003 HSP 2 Score: 88.1965 bits (217), Expect = 2.827e-17 Identity = 58/202 (28.71%), Postives = 97/202 (48.02%), Query Frame = -1
Query: 2025 GPSGNVYRLNSELLSKYGSDTSQESISGYV------VELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLD-GDVKIKY-NGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPEC-----IDVKDNIL------RKGDTVI 2573
G G Y+ + L ++ QES G+ V+ + + RQ+Q GHG WN M + I + + +H+ D GDV+++Y N + +P A+ N F +GE + V+T+K+LQ HG W+D + G+ ++++++ DGD+ V+ G W NP C +DV N++ G TVI
Sbjct: 344 GQGGFYYKDHLPKLGEHAELQRQESADGHSFQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICR-IGTVHRITDRGDVRVQYSNNIRWTFHPGALTKVN----------TFGVGELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPVDVDANLMTTENPNESGSTVI 534 HSP 3 Score: 75.485 bits (184), Expect = 2.085e-13 Identity = 39/136 (28.68%), Postives = 69/136 (50.74%), Query Frame = -1
Query: 1863 HKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFG-MEWMINPECIDVKDNILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNGLGLVYNP 2267
H F+ G++ + VD ++++QE HGGW+ + Y + + I + GD+ V + W +P + K N G+ V + D+++ K+LQ HG W M+ G+ GK+ + GD++V G +NP
Sbjct: 372 HSFQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGAL-TKVNTFGVGELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNP 506 HSP 4 Score: 73.559 bits (179), Expect = 8.863e-13 Identity = 40/141 (28.37%), Postives = 75/141 (53.19%), Query Frame = -1
Query: 2298 DVVIAIKDLETIIQMQMAHGGWNDSMNE--CLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASK 2714
D V + +++ + QMQ HGGWN M E C IG + G D V+ + + + L+K + G +V ++ + + ++LQ GHGEW D+M ++G+ +V+ + DGD+++ + G T+ NP ++++
Sbjct: 378 DKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRG-DVRVQYSNNIRWTFHPGALTKVNTFGV-----GELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQ 512
BLAST of SMED30011819 vs. Ensembl Medaka
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:101158637]) HSP 1 Score: 278.485 bits (711), Expect = 5.604e-79 Identity = 202/672 (30.06%), Postives = 337/672 (50.15%), Query Frame = -1
Query: 120 LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNV-NGLGLVYNPX-----------XXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCES--VGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFL-KREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMG--------ILGDMTEGSD--TPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQ-DLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2048
L+ GD V + D++ + LQ HGGW M G + I D+ V +G +NP + + F GD V++ D ++KLLQ+ HGEW +M LGK G+V+ +++D DL+V++ + T NP V + + + E LS+L+ + + D EE++K A NG+L V ++ + V V+ + G + A GH+DV+KLL++ +L A+DKDGD A+H A ++ + ++L GAD+NA+N + TP+ +A + G +++ + L S+ DS+ DT LH A+ K R + + +LL+AGAD+ N FN H AAL+G+ + + L K IVD +K+D + A+HL ALNNH + +L+ A ++I+N QTALH+AV ++H V++LV A+++++D DGDTPLH A++ + +++ M + + I+G T+G++ + IA +L +GA L ++NKKG +P+++C D PN K KE + G R P++ S E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 247 LQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFHVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKIAPSGSAVSN--ASGERLSQLLKKLFETQESGDI------------NEELVK---AAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHNVDLEAEDKDGDRAVHHAAFGDEGSVIEVLQRGGADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNASLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPD------------PNLCKALAKCHKEKSSGQVGTR-------------SPSLNSNMESLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 862 HSP 2 Score: 79.7221 bits (195), Expect = 1.110e-14 Identity = 66/193 (34.20%), Postives = 96/193 (49.74%), Query Frame = -1
Query: 2193 GLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDA--IVRGPSGNVYRLNSELLSKYGSDTSQESISG--------YVVELIQ---DEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENH 2732
GL+ GD V I + DLE + +Q HGGW D M E L V GI D +V+ PSGN + N +L+K S E +G +V +L+Q D + + LQ GHGEW + M +GK +V + D D+K++ G+++ NP A+ P S V + GER S++ +KKL E
Sbjct: 246 GLQIGDLVNIDL-DLEIVQSLQHGHGGWTDGMFETLT-TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFHVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKI--APSGSAVSNAS--GERLSQL-----LKKLFETQ 427 HSP 3 Score: 66.6254 bits (161), Expect = 9.408e-11 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = -1
Query: 117 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 299
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 960 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1020 HSP 4 Score: 65.4698 bits (158), Expect = 2.338e-10 Identity = 64/242 (26.45%), Postives = 108/242 (44.63%), Query Frame = -1
Query: 1893 GNVYRLNSELLSKYGSDTSQESIS-GYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKY-NGSTFVINPKAIASKNEKPPVSEVC-------HKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECIDVKDNILRKGDTVI------LSDDIQKTKQLQQNHGGWVSRMSVFA---GKCGKIEAILPTGDVKVN 2564
G+ YR + +L + + + + G +V + D + + LQ GHG W D M + + V D D+ ++Y +G+ + NP A+ +K EV +F +G+ ++D +K LQ HG W++ ++ GK + +I+ D D+ V G W NP + I G V LS ++K + Q++ G ++ V A G K+E IL DV VN
Sbjct: 225 GSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNP-AVLTKANVVRSGEVAAGAEGGSSQFHVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV---TKIAPSGSAVSNASGERLSQLLKKLFETQES--GDINEELVKAAANGDLAKVEDILKRPDVDVN 460
BLAST of SMED30011819 vs. Planmine SMEST
Match: SMESG000017281.1 (SMESG000017281.1) HSP 1 Score: 1672.14 bits (4329), Expect = 0.000e+0 Identity = 867/879 (98.63%), Postives = 873/879 (99.32%), Query Frame = -1
Query: 114 MGASLSGLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECIDVKDNILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNGLGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLYK 2750
MGASLSGLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWN+NMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASKNEKPPVSEVCHKFKIGE+ IGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECIDVKDN LRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNGLGLVYNPLLLTKSDKSDLKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQN+TLNPECVEATDSDSDS+PDIFSMMEHLSKLMNLDSDQSDQEDKGKDSDDGKTDGNQEEVMKLFFAVKNGELNNVRNLCESVGV+ARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVEN AEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSL+IPNSTKQDLIEKEPNPGTKAER EAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLYK
Sbjct: 1 MGASLSGLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNNNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASKNEKPPVSEVCHKFKIGEKVVLIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECIDVKDNKLRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNGLGLVYNPLLLTKSDKSDLKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNLTLNPECVEATDSDSDSDPDIFSMMEHLSKLMNLDSDQSDQEDKGKDSDDGKTDGNQEEVMKLFFAVKNGELNNVRNLCESVGVNARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENYAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLNIPNSTKQDLIEKEPNPGTKAERFEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLYK 879
BLAST of SMED30011819 vs. Planmine SMEST
Match: SMESG000017279.1 (SMESG000017279.1) HSP 1 Score: 996.497 bits (2575), Expect = 0.000e+0 Identity = 524/911 (57.52%), Postives = 657/911 (72.12%), Query Frame = -1
Query: 114 MGASLSGLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI------DVKDNILRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNGLGLVYNP--------------------XXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDL------LKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLYK 2750
MG S + VVI KD+ETI Q HGGWN +MNECL GPC+LVQ +GS AIV+GPSGNVYRLNSELL +Y DT Q+ G V LI++ ++F++LQV +GEW+D+M +I+GKKV++I +F DG+VKIKY G TF IN KAI + N K E+ F+ GE+ I N + MK LQ++HGGW +L+ L+ G+EVEIV+I +DGDMYVS+ GMEWMINP+CI K LRKGD V + DDIQK KQLQQNHGGWV+ M +GK K+++ILP+GDVKVNV+ L LVYNP + +K ++K+F+PGDKVRLIN+K K+K LQK HGEWV++M+F LGK GKVKS+ +GDL+VKIM Q + LNPECVEATDSD +SEPDI +M+ L+++M D D + + + F A+K G LN VR L +SVG L+G+YPLMIAVNEGHIDV+KLLIEKRAN+NAK+++GDTALH+AV K++ KICDLLINKGADVNAQNNNKITPIMLAAS+GS++ITRLLATSRNADLSIG+S+NDTALHLAV+ SV +LLDAGADI A+N K FN FHL A +G+ +ILE+FLKREGVKIV+SQK+DKFAA+HL NNHE CLRVLL YKADINIRNN LQTALHIAV QK+LNC+KILVE AEI++ D++GDT LHIA+++ ++ KK G++ G+ + EGSD P+D+AIYLLK A D+KNK TPM+ C+ L AR +S +I +++ + + A L A + ++K++DEELEELRQRIQDMEDE+TCIIC+DR NVTFGCGHVACDVCANNLYECHICRE+I NKIRLY+
Sbjct: 1 MGGVESVENSNIQVVIGTKDMETIKIKQREHGGWNANMNECLTGPCQLVQSLGSCAIVKGPSGNVYRLNSELLIQY--DTRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILTDNCKKTSMEMEETFEEGEKVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARAQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMG-----------QSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEGVYPLMIAVNEGHIDVIKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNVLLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTALHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDRTPMDFCDPSLKEKLSVANARRMSKEIETK----ILQAQGSTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDERTCIICFDRQNNVTFGCGHVACDVCANNLYECHICRENIGNKIRLYR 894
BLAST of SMED30011819 vs. Planmine SMEST
Match: SMESG000031930.1 (SMESG000031930.1) HSP 1 Score: 549.28 bits (1414), Expect = 0.000e+0 Identity = 305/639 (47.73%), Postives = 408/639 (63.85%), Query Frame = -1
Query: 120 TVILSDDIQKTKQLQQNHGGWVSRM-SVFAGKCGKIEAILPTGDVKVNVNGLGLVYNPXXXXXXXXXXXKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNVTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFFAVKNGELNNVRNLCESVGVDARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLDIPNSTKQDLIEKEPNPGTKAERLEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRL 2033
VI + D+ + Q+NHGGW M G C + I G + G++Y +K G V+LI D+ + KLLQ HGEW M LGK+ +V +F DGD ++ N+ ++P+ +++ ++ S S +L E M+ A+K G L+ V L +SVGV+ LKG+YPL+IA NEGH D+V LL+EK+AN+N DKDGDTALHLAV +N+ K+CDLL+ KGADVNAQNN KITP M+A GS +I LL TS NADLSIGDSD DTALHLAV SR+ SVE LL+AGADI LNKKLFN +HLAA+ GH KILE+FLKR+G K+V+SQKEDKFAAIH+ A+NNHE CL VL+ YKAD+N++N L+TALHIAV +K+L+ VK LVEN AE++L+D +G+T LH+AMKV Q E+ G M S +G+LG++ EG D +DIAIYL+++GA +D+KN KG TP+++CND+ LK RILSL +S K + TK E LE P+ P + +S + EL ELRQ++++MEDEK C IC+DR + V F CGH+ CD C++N+ ECH+C E I +KI++
Sbjct: 14 VVIANQDLSTIIKKQKNHGGWNDLMKECLTGPC---KLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKSDDSDDSDDSNDRSLI--------------------TDERTMRFLVALKTGNLSEVLRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTES--GFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHI--------TKKELLEHPV-------QPSDARSNEAELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKI 612 HSP 2 Score: 164.466 bits (415), Expect = 3.588e-42 Identity = 80/151 (52.98%), Postives = 104/151 (68.87%), Query Frame = -1
Query: 2298 MGASLSGLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASK 2750
MGA++S VVIA +DL TII+ Q HGGWND M ECL GPCKL+ GSDA V GPSG +YRLNSELL + S + G V+LIQDE++F+ LQ HGEW ++M++I+GK+V+VI F DGD KI +N F+++PK I S+
Sbjct: 1 MGANISSFDIRKKVVIANQDLSTIIKKQKNHGGWNDLMKECLTGPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHK--IGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSE 149 HSP 3 Score: 60.8474 bits (146), Expect = 4.259e-9 Identity = 44/152 (28.95%), Postives = 72/152 (47.37%), Query Frame = -1
Query: 2067 GSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNDNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASKNEKPPVSEVCHKFKIGERWSEIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI 2522
G++ S I VV QD + Q HG WND MK+ + ++I+ + +G + +N + + P V K KIG I + + K LQ HG W + + GKEVE+++IF+DGD +S ++++P+ I
Sbjct: 2 GANISSFDIRKKVVIANQDLSTIIKKQKNHGGWNDLMKECLTGPCKLINDDGSDATVMGPSGMIYRLNSELLV------PFKSV-KKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTI 146
BLAST of SMED30011819 vs. Planmine SMEST
Match: SMESG000030607.1 (SMESG000030607.1) HSP 1 Score: 197.208 bits (500), Expect = 2.091e-51 Identity = 152/488 (31.15%), Postives = 250/488 (51.23%), Query Frame = -1
Query: 450 FAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQN------VTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFF--------------AVKNGELNNVRNLCE--SVGVDARLKGIYPLMIAVNEGHIDVVKLLIEK---RANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVK-IVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGS-LMGI-LGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCND-DLLKA 1823
+PG V++ +D +K +Q G W SM +G GK+ RV + + LN + S S P++ S+ + M L + + ++ + + G V++ F A NG+L+ V L + SV V++ L A GH+++V+ LI+ + ++N KD DGD A+H A + I +L N GAD+N++N TP+ +A + G +E+ + L ++A + D D DT LH ++ + L+ A +DI N FN H AAL+G+ + L+ + IVD +K+D + A+HL ALNNH D +L+ AD+N++N LQT LH+AV ++H N VK+LV++ A+I++ D DGDTPLH A++ + ++M + MG LMG+ LG + IA YL+ +G+ + +KNKKG TP+++C D +L++A
Sbjct: 402 LSPGCWVKISSDLELIKTVQCGLGMWNESMAVCVGTVGKIVQSLGHDLWRVVLSRTSGSSESTFVLNSALLARIRSPM-SSPEV-SVSATQPRTMLLSQRSTGSTSQPIEAVEAASTGTLSHVLRSIFEAHTTGDAKELFVKAAANGDLDKVLELLKKRSVDVNSMFARHSALHAASQNGHVEIVQALIKYGGDQLDINLKDPDGDAAVHHAALGDHKIIIQILKNAGADINSRNRRNQTPLHIAVNKGHLEVLKALLELKSALQTNQDYDGDTPLHDSITRRHDKLTLALVQANSDITVCNNAGFNSLHHAALRGNPNATRIILENLPRQWIVDEKKDDGYTALHLAALNNHADVAELLIKVGSADLNVQNINLQTPLHLAVERQHSNMVKLLVDSGADISIPDKDGDTPLHEALRHHTLSQLRQMQDLQDMGKLLMGLSLGGEKRSA---AHIACYLVSNGSDIHIKNKKGQTPLDLCPDQNLIRA 884 HSP 2 Score: 72.0182 bits (175), Expect = 1.845e-12 Identity = 72/261 (27.59%), Postives = 120/261 (45.98%), Query Frame = -1
Query: 696 PLMIAVNEGHIDVVKLLIEKRANLNA-KDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDND--TALHLAVRKSRVNSVEILLDAG-ADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNH-----------EDCLRVLLAYK-------------------ADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINL 1376
PL IAVN+GH++V+K L+E ++ L +D DGDT LH ++ + K+ L+ +D+ NN + AA G+ TR++ + + + +D TALHLA + + E+L+ G AD+N N L HLA + H+ +++L + G I K D +H AL +H +D ++L+ +DI+I+N QT L + Q + +K EN+ N+
Sbjct: 640 PLHIAVNKGHLEVLKALLELKSALQTNQDYDGDTPLHDSITRRHDKLTLALVQANSDITVCNNAGFNSLHHAALRGNPNATRIILENLPRQWIVDEKKDDGYTALHLAALNNHADVAELLIKVGSADLNVQNINLQTPLHLAVERQHSNMVKLLVD-SGADISIPDK-DGDTPLH-EALRHHTLSQLRQMQDLQDMGKLLMGLSLGGEKRSAAHIACYLVSNGSDIHIKNKKGQTPLDLCPDQNLIRALKRCHENSQPPNV 897 HSP 3 Score: 67.0106 bits (162), Expect = 6.000e-11 Identity = 27/64 (42.19%), Postives = 42/64 (65.62%), Query Frame = -1
Query: 117 RDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 308
+D++ ++L Q+ QD+++ C +C DR + F CGH +C +C + L C ICRE+I NKI LY
Sbjct: 1230 KDKDYKKLEQQYQDIKEMTMCPVCMDRVRKMVFMCGHCSCQLCGDKLETCPICRETITNKILLY 1293
BLAST of SMED30011819 vs. Planmine SMEST
Match: SMESG000030607.1 (SMESG000030607.1) HSP 1 Score: 194.512 bits (493), Expect = 1.611e-50 Identity = 152/488 (31.15%), Postives = 249/488 (51.02%), Query Frame = -1
Query: 450 FAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQN------VTLNPECVEATDSDSDSEPDIFSMMEHLSKLMNLXXXXXXXXXXXXXXXXXXXXGNQEEVMKLFF--------------AVKNGELNNVRNLCE--SVGVDARLKGIYPLMIAVNEGHIDVVKLLIEK---RANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVK-IVDSQKEDKFAAIHLVALNNHEDCLRVLLAY-KADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGS-LMGI-LGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCND-DLLKA 1823
+PG V++ +D +K +Q G W SM +G GK+ RV + + LN + S S P++ S+ + M L + + ++ + + G V++ F A NG+L+ V L + SV V++ L A GH+++V+ LI+ + ++N KD DGD A+H A + I +L N GAD+N++N TP+ +A + G +E+ + L + S+ D D DT LH ++ + L+ A +DI N FN H AAL+G+ + L+ + IVD +K+D + A+HL ALNNH D +L+ AD+N++N LQT LH+AV ++H N VK+LV++ A+I++ D DGDTPLH A++ + ++M + MG LMG+ LG + IA YL+ +G+ + +KNKKG TP+++C D +L++A
Sbjct: 402 LSPGCWVKISSDLELIKTVQCGLGMWNESMAVCVGTVGKIVQSLGHDLWRVVLSRTSGSSESTFVLNSALLARIRSPM-SSPEV-SVSATQPRTMLLSQRSTGSTSQPIEAVEAASTGTLSHVLRSIFEAHTTGDAKELFVKAAANGDLDKVLELLKKRSVDVNSMFARHSALHAASQNGHVEIVQALIKYGGDQLDINLKDPDGDAAVHHAALGDHKIIIQILKNAGADINSRNRRNQTPLHIAVNKGHLEVLKALLELK-CLASLQDYDGDTPLHDSITRRHDKLTLALVQANSDITVCNNAGFNSLHHAALRGNPNATRIILENLPRQWIVDEKKDDGYTALHLAALNNHADVAELLIKVGSADLNVQNINLQTPLHLAVERQHSNMVKLLVDSGADISIPDKDGDTPLHEALRHHTLSQLRQMQDLQDMGKLLMGLSLGGEKRSA---AHIACYLVSNGSDIHIKNKKGQTPLDLCPDQNLIRA 883 HSP 2 Score: 72.7886 bits (177), Expect = 1.009e-12 Identity = 71/260 (27.31%), Postives = 119/260 (45.77%), Query Frame = -1
Query: 696 PLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDND--TALHLAVRKSRVNSVEILLDAG-ADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNH-----------EDCLRVLLAYK-------------------ADINIRNNILQTALHIAVLQKHLNCVKILVENNAEINL 1376
PL IAVN+GH++V+K L+E + + +D DGDT LH ++ + K+ L+ +D+ NN + AA G+ TR++ + + + +D TALHLA + + E+L+ G AD+N N L HLA + H+ +++L + G I K D +H AL +H +D ++L+ +DI+I+N QT L + Q + +K EN+ N+
Sbjct: 640 PLHIAVNKGHLEVLKALLELKCLASLQDYDGDTPLHDSITRRHDKLTLALVQANSDITVCNNAGFNSLHHAALRGNPNATRIILENLPRQWIVDEKKDDGYTALHLAALNNHADVAELLIKVGSADLNVQNINLQTPLHLAVERQHSNMVKLLVD-SGADISIPDK-DGDTPLH-EALRHHTLSQLRQMQDLQDMGKLLMGLSLGGEKRSAAHIACYLVSNGSDIHIKNKKGQTPLDLCPDQNLIRALKRCHENSQPPNV 896 HSP 3 Score: 67.0106 bits (162), Expect = 5.948e-11 Identity = 27/64 (42.19%), Postives = 42/64 (65.62%), Query Frame = -1
Query: 117 RDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 308
+D++ ++L Q+ QD+++ C +C DR + F CGH +C +C + L C ICRE+I NKI LY
Sbjct: 1229 KDKDYKKLEQQYQDIKEMTMCPVCMDRVRKMVFMCGHCSCQLCGDKLETCPICRETITNKILLY 1292 The following BLAST results are available for this feature:
BLAST of SMED30011819 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of SMED30011819 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of SMED30011819 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of SMED30011819 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of SMED30011819 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of SMED30011819 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of SMED30011819 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of SMED30011819 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30011819 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of SMED30011819 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of SMED30011819 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of SMED30011819 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of SMED30011819 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of SMED30011819 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30011819 ID=SMED30011819|Name=SMED30011819|organism=Schmidtea mediterranea sexual|type=transcript|length=2819bpback to top protein sequence of SMED30011819-orf-1 >SMED30011819-orf-1 ID=SMED30011819-orf-1|Name=SMED30011819-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=880bp MGASLSGLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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