G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30011813
Length (bp)1179
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30011813) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30011813) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30011813

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30011813h1SMcG0001407 dd_Smed_v6_27761_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Match: galr2a (galanin receptor 2a [Source:ZFIN;Acc:ZDB-GENE-130603-37])

HSP 1 Score: 50.0618 bits (118), Expect = 2.625e-6
Identity = 39/145 (26.90%), Postives = 69/145 (47.59%), Query Frame = 3 
Query:  624 CFFIFQSVLPVLVVGGISAGMTYSLIKEPNPQLLRPQNNTLSQKSYNKTSNIRGSSQRKATKTIFVLAIVFTTLEAPVFIIQILIQNGTFAFILNSNPHVVYFIINFLVVLDSCSNFMVYLVTSDKFRAEFRRLIWRSGCYSQGR 1058
            C FIF  ++PVL++G   A     L    +P     Q+ + S+K+           +RK TK I ++A++F     P  ++ + +  G F   LN   +V+  + + +   +SC N +VY + S  FR  F+++    GC  + R
Sbjct:  183 CTFIFGYLIPVLILGITYARTIRYLWTSVDPM----QDMSESRKA-----------KRKVTKMIIIVAVLFCLCWLPHHLVILCMWFGHFP--LNHTTYVLRILSHLVAYANSCLNPIVYALVSKHFRKGFKKVF---GCAFRNR 307
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Match: galr2a (galanin receptor 2a [Source:ZFIN;Acc:ZDB-GENE-130603-37])

HSP 1 Score: 49.6766 bits (117), Expect = 3.007e-6
Identity = 39/145 (26.90%), Postives = 69/145 (47.59%), Query Frame = 3 
Query:  624 CFFIFQSVLPVLVVGGISAGMTYSLIKEPNPQLLRPQNNTLSQKSYNKTSNIRGSSQRKATKTIFVLAIVFTTLEAPVFIIQILIQNGTFAFILNSNPHVVYFIINFLVVLDSCSNFMVYLVTSDKFRAEFRRLIWRSGCYSQGR 1058
            C FIF  ++PVL++G   A     L    +P     Q+ + S+K+           +RK TK I ++A++F     P  ++ + +  G F   LN   +V+  + + +   +SC N +VY + S  FR  F+++    GC  + R
Sbjct:  184 CTFIFGYLIPVLILGITYARTIRYLWTSVDPM----QDMSESRKA-----------KRKVTKMIIIVAVLFCLCWLPHHLVILCMWFGHFP--LNHTTYVLRILSHLVAYANSCLNPIVYALVSKHFRKGFKKVF---GCAFRNR 308
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Match: galr2a (galanin receptor 2a [Source:ZFIN;Acc:ZDB-GENE-130603-37])

HSP 1 Score: 49.6766 bits (117), Expect = 3.007e-6
Identity = 39/145 (26.90%), Postives = 69/145 (47.59%), Query Frame = 3 
Query:  624 CFFIFQSVLPVLVVGGISAGMTYSLIKEPNPQLLRPQNNTLSQKSYNKTSNIRGSSQRKATKTIFVLAIVFTTLEAPVFIIQILIQNGTFAFILNSNPHVVYFIINFLVVLDSCSNFMVYLVTSDKFRAEFRRLIWRSGCYSQGR 1058
            C FIF  ++PVL++G   A     L    +P     Q+ + S+K+           +RK TK I ++A++F     P  ++ + +  G F   LN   +V+  + + +   +SC N +VY + S  FR  F+++    GC  + R
Sbjct:  184 CTFIFGYLIPVLILGITYARTIRYLWTSVDPM----QDMSESRKA-----------KRKVTKMIIIVAVLFCLCWLPHHLVILCMWFGHFP--LNHTTYVLRILSHLVAYANSCLNPIVYALVSKHFRKGFKKVF---GCAFRNR 308
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183SJQ7 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS4455 PE=4 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 1.722e-25
Identity = 102/343 (29.74%), Postives = 177/343 (51.60%), Query Frame = 3 
Query:  126 FSILASLANGVSVVILSRPNSSQRFGQIRLNLLILSISETSLNAFITVFNLTHIVNISQYSRIGALWIDTMLFWNITSLICNRNWIVILIACSRCEIVFLPLHSVSG---LKFNNKVIRNXXXXXXXXXXXXXXVRLFSKKAIVCTNIDE--VKLQPHLSGQWAYSVYETYCFFIFQSVLPVLVVGGISAGMTYSLIK------------EPNPQLLRPQ-------NNTLSQKSYNKTSNIRGSSQRKATKTIFVLAIVFTTLEAPVFIIQILIQNGTFAFILNSNPHVVYFIINFLVVLDSCSNFMVYLVTSDKFRAEFRRLIWRSGCYSQGRQRSVINDN 1082
            F+I+  L   +++++L+R N ++RFG +R +L+ L+  E  LN  I V  +           + A  +  + F  + S IC+RNW V LIA SRCE +  PL   S    L  N ++I  L + +  ++ + S++RL     +VCTN++   ++L P  +      ++E   FF +QS +P+ +V   +  M ++L++            +  PQL R         +  +  + Y K +  R ++Q +AT+TIF +A VFT LEAPVF I I+ +N     +      V+  ++ FL++LDS +N ++YL+TS+ FR E  R++   G Y   +  S + + 
Sbjct:   33 FAIMLEL---IALIVLAR-NGAKRFGSMRKSLIALTSIEMWLNTSIFVHKVWEDYGSPHPETLLAYILAAISFSLLNSAICSRNWCVTLIALSRCEAITRPLAHRSSKQILSPNRQII--LLVCVVTLAMVFSAIRLTFLSIVVCTNLEHKVIRLPP--TNSTNAQIFEK-VFFAYQSAIPISIVALATVFMIWTLLRPRIESAKKSPSAKAQPQLQRQSTVSGTNNHGKILPRGYEKKNAPRLTNQVRATRTIFFIAAVFTILEAPVFFI-IVFENHIGIEV----KKVIVQLLKFLIILDSYTNIVIYLLTSEHFRTELIRML-TCGRYKTDKVHSRVRNT 360
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A3P7M935 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS14106 PE=4 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 5.153e-23
Identity = 106/344 (30.81%), Postives = 178/344 (51.74%), Query Frame = 3 
Query:  126 FSILASLANGVSVVILSRPNSSQRFGQIRLNLLILSISETSLNAFITVFNLTHIVNISQYSRIGALWIDTMLFWNITSLICNRNWIVILIACSRCEIVFLPLHSVSG---LKFNNKVIRNXXXXXXXXXXXXXXVRLFSKKAIVCTNIDE--VKLQPHLSGQWAYSVYETYCFFIFQSVLPVLVVGGISAGMTYSLIKEP---------NPQLLRP-----------QNNTLSQKSYNKTSNIRGSSQRKATKTIFVLAIVFTTLEAPVFIIQILIQNGTFAFILNSNPHVVYF-IINFLVVLDSCSNFMVYLVTSDKFRAEFRRLIWRSGCYSQGRQRSVIND 1079
            F+IL  L   +++++L+R + ++RFG +R +L+ L+  E  LN  I V  +           + +  +  +LF  + S IC+RNW V LIA SRCE +  PL   S    L  N ++I  L + + I+  + S+VRL     +VCTN++   ++L P  S       +E   FF +QS +P+ +V   +  M ++L++           +P++ R             +  +  + Y K +  R ++Q +AT+TIF +A VFT LEAPVF I I+ +N      + S+  VV   ++ FL++LDS +N  +YL+TS+ FR E  R++   G Y   +  S I +
Sbjct:   33 FAILLEL---IALIVLAR-HRAKRFGSMRKSLIALTSIEMWLNTSIFVHKVWEDYGSPHPETLLSYILAAILFSLLNSAICSRNWCVTLIALSRCEAITRPLAHRSSKQLLSPNRQLI--LLVFVIILGIIFSAVRLTFLNIVVCTNLNNTVIRLPPANSTNAGR--FEK-VFFAYQSAIPISIVALATVFMIWTLLRPKIAAAKRTPSSPEIQRQLQCPSTAHGSHNHGKILPRGYEKRNVSRLTNQVRATRTIFFIAAVFTVLEAPVFFI-IVFENH-----IGSDVRVVIVQLLKFLIILDSYTNIAIYLLTSEHFRTELIRML-TCGRYRTEKAHSRIRN 360
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0R3T1S6 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS843 PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 7.190e-22
Identity = 92/349 (26.36%), Postives = 177/349 (50.72%), Query Frame = 3 
Query:   30 MFLISFSNNCVFENITRPTLASKPIYSYM---MILFSILASLANGVSVVILSRPNSSQRFGQIRLNLLILSISETSLNAFITVFNLTHIVNISQYSRIGALWIDTMLFWNITSLICNRNWIVILIACSRCEIVFLPL-HSVSGLKF--NNKVIRNXXXXXXXXXXXXXXVRLFSKKAIVCTNIDEVKLQPHLSGQWAYSVYETYCFFIFQSVLPVLVVGGISAGMTYSLIKEPNPQLLRPQNNTLSQKSYNK-----------TSNIRGSSQRKATKTIFVLAIVFTTLEAPVFIIQILIQNGTFAFILNSNPHVVYFIINFLVVLDSCSNFMVYLVTSDKFRAEFRRL 1025
            M  +++ NNC+       T     ++S+M   +++FS++A +   +++V+L     S+RFG +R ++++L+  E  LN  I +        + ++          +LF  + + IC+RNW V LIA +RCE++  P+ H V+   F  N ++I     ++  +  +LS +RL  +  IVCTN+D +   P ++      + E   FF +QS +P+ +V   +  M  +L++  + Q++R +N++  + S  K             +   S   +AT+ I ++  +F   EAP+F   +L ++     I       VY  + FL+V D+ +NF++YL+TS  FR   +++
Sbjct:    1 MTFVTYRNNCLVTPPVATTTKHGELFSFMPVALLIFSMIALILEVIAIVVLCH---SRRFGSLRKSVIVLTSIEMWLNCSIFINKAWEGFGMPKHRTFAGFICSFLLFGLLNNAICSRNWAVTLIAFARCEVITRPVAHRVNTHIFSPNRQII--YISILIFVGMVLSFLRLLFRPVIVCTNLDGLLQYPEINVTQLQIINEK-VFFAYQSAIPIFLVSLATIFMMITLLR--SSQIVR-KNSSEPKNSDGKIISCVYQQKKKKKSYHLSKHVRATRAILLIIGIFIVCEAPIFFAVVLEKH-----ISPEAKLTVYKALRFLIVTDTYANFIIYLLTSKHFRYGLKKV 335
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0R3UKE8 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS8061 PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 9.471e-22
Identity = 93/327 (28.44%), Postives = 162/327 (49.54%), Query Frame = 3 
Query:  108 SYMMIL-FSILASLANGVSVVILSRPNSSQRFGQIRLNLLILSISETSLNAFITVFNLTHIVNISQYSRIGALWIDTMLFWNITSLICNRNWIVILIACSRCEIVFLPLHS-VSGLKFNNKVIRNXXXXXXXXXXXXXXVRLFSKKAIVCTNIDEVKLQPHLSGQWAYSVYETYCFFIFQSVLPVLVVGGISAGMTYSLIKEPNP---------QLLRPQNNTLSQKSYNKTSNIRGSSQRKATKTIFVLAIVFTTLEAPVFIIQILIQNGTFAFILNSNPHVVYFIINFLVVLDSCSNFMVYLVTSDKFRAEFRRLIWRSGCYSQG 1055
            +Y+ +L FS +A L    ++ +L R +S+ RFG +R +L+ L++ E  LN  I V          ++           LF  + S IC+RNW+V LIA +RCE +  P+ S VS   F+ K     +  + I  ++LS++RL  ++ +VCTN++   +Q   +  W   V E   FF +QS +P+ +V   +  M   L++   P            R + + +    +      R  +Q +AT+ I ++A VF   EAPVF   + ++      ++      ++  + FL+V DS +NF++YL+T   FR E  RL+    C+++ 
Sbjct:   47 AYIALLAFSAIAVLFELTALTVLGR-SSAVRFGSMRKSLVTLTVIEMWLNTSIFVHKAWEEFATMRHETFSEFCFSFFLFSILNSAICSRNWVVTLIALARCEAITRPVASRVSTHIFSPKRQLMYTATLIICGFVLSTLRLVFRQMLVCTNLNNAVVQVEANRTWVQDVSEKV-FFAYQSAIPITIVTISTLFMIVILLRHRMPFEKKGSIRQAQCRDERSCVVSSLHEVRQAQRLPNQIRATRFILLIATVFIVCEAPVFFAVVFVEAIPRNAVIG-----IFKYLRFLIVADSFANFVIYLLTIKPFRLELVRLL---SCHTKS 363
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0R3WEV8 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS9373 PE=4 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 2.196e-21
Identity = 93/339 (27.43%), Postives = 163/339 (48.08%), Query Frame = 3 
Query:  117 MILFSILASLANGVSVVILSRPNSSQRFGQIRLNLLILSISETSLNAFITVFNLTHIVNISQYSRIGALWIDTMLFWNITSLICNRNWIVILIACSRCEIVFLPLHS-VSGLKFNNKVIRNXXXXXXXXXXXXXXVRLFSKKAIVCTNIDEVKLQPHLSGQ---WAYSVYETYCFFIFQSVLPVLVVGGISAGMTYSLIKEPNPQLLRPQNN-----TLSQKSYNKTSNIRGS--------------------------SQRKATKTIFVLAIVFTTLEAPVFIIQILIQNGTFAFILNSNPHVVYFIINFLVVLDSCSNFMVYLVTSDKFRAEFRRLI 1028
            +++F+ +A +   V++ +L+RP ++ RFG ++ +L+ L+  E  LN  I V         +++ ++       +LF  + S IC+RNW+V LIA +RCE +  P+ + VS   F+         ++ + S  LS+ RL  ++ +VCTN+D V L  H+S     W   + E   FF +QS +P+ VV   +  M   L++   P   R +         ++K   K    RG                           +Q +AT+ I  +A VF   EAPVF   + + + + + ++      V+  + FL++ DS +NF++YL+TS  FR E   L+
Sbjct:   57 LLVFAAIAVVLEAVALTVLTRP-AATRFGSMQRSLVALTAMEMWLNTTIFVHKAWEEYFTTRHEKLSEFCFSFILFSGLNSAICSRNWVVTLIALARCEAITRPVATRVSTHIFSPHRQLVYIGILIVCSIALSTFRLVIRQVLVCTNLDNVVL--HVSSDQISWLQVISEKI-FFAYQSAIPITVVTIATLFMIVVLLRHRVPSERRKEQQRKVEAKPAEKEGRKWKCWRGGRSTPRDEGGGCVVPSLQAVRQAQRLPNQMRATRFILFIATVFIVCEAPVFFAVVCVNSMSPSVVIR-----VFTYLRFLIIADSFANFVIYLLTSRPFRVELVNLL 386
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000078733.1 (SMESG000078733.1)

HSP 1 Score: 724.546 bits (1869), Expect = 0.000e+0
Identity = 375/376 (99.73%), Postives = 376/376 (100.00%), Query Frame = 3 
Query:   30 MFLISFSNNCVFENITRPTLASKPIYSYMMILFSILASLANGVSVVILSRPNSSQRFGQIRLNLLILSISETSLNAFITVFNLTHIVNISQYSRIGALWIDTMLFWNITSLICNRNWIVILIACSRCEIVFLPLHSVSGLKFNNKVIRNXXXXXXXXXXXXXXVRLFSKKAIVCTNIDEVKLQPHLSGQWAYSVYETYCFFIFQSVLPVLVVGGISAGMTYSLIKEPNPQLLRPQNNTLSQKSYNKTSNIRGSSQRKATKTIFVLAIVFTTLEAPVFIIQILIQNGTFAFILNSNPHVVYFIINFLVVLDSCSNFMVYLVTSDKFRAEFRRLIWRSGCYSQGRQRSVINDNFSVTEQLIVTQSQKMASIIHRIRPD 1157
            MFLISFSNNCVFENITRPTLASKPIYSYMMILFSILASLANGVSVVILSRPNSSQRFGQIRLNLLILSISETSLNAFITVFNLTHIVNISQYSRIGALWIDTMLFWNITSLICNRNWIVILIACSRCEIVFLPLHSVSGLKFNNKVIRNLSILIFIISYLLSSVRLFSKKAIVCTNIDEVKLQPHLSGQWAYSVYETYCFFIFQSVLPVLVVGGISAGMTYSLIKEPNPQLLRPQNNTLSQKSY+KTSNIRGSSQRKATKTIFVLAIVFTTLEAPVFIIQILIQNGTFAFILNSNPHVVYFIINFLVVLDSCSNFMVYLVTSDKFRAEFRRLIWRSGCYSQGRQRSVINDNFSVTEQLIVTQSQKMASIIHRIRPD
Sbjct:    1 MFLISFSNNCVFENITRPTLASKPIYSYMMILFSILASLANGVSVVILSRPNSSQRFGQIRLNLLILSISETSLNAFITVFNLTHIVNISQYSRIGALWIDTMLFWNITSLICNRNWIVILIACSRCEIVFLPLHSVSGLKFNNKVIRNLSILIFIISYLLSSVRLFSKKAIVCTNIDEVKLQPHLSGQWAYSVYETYCFFIFQSVLPVLVVGGISAGMTYSLIKEPNPQLLRPQNNTLSQKSYSKTSNIRGSSQRKATKTIFVLAIVFTTLEAPVFIIQILIQNGTFAFILNSNPHVVYFIINFLVVLDSCSNFMVYLVTSDKFRAEFRRLIWRSGCYSQGRQRSVINDNFSVTEQLIVTQSQKMASIIHRIRPD 376
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000031763.1 (SMESG000031763.1)

HSP 1 Score: 350.517 bits (898), Expect = 6.976e-119
Identity = 202/383 (52.74%), Postives = 260/383 (67.89%), Query Frame = 3 
Query:   30 MFLISFSNNCVFENITRPTLASKPIYSYMMILFSILASLANGVSVVILSRPNSSQRFGQIRLNLLILSISETSLNAFITVFNLTHIVNISQYSRIGALWIDTMLFWNITSLICNRNWIVILIACSRCEIVFLPLHSVSGLKFNNKVIRNXXXXXXXXXXXXXXVRLFSKKAIVCTNIDEVKLQPHLSGQWAYSVYETYCFFIFQSVLPVLVVGGISAGMTYSLIKEPNPQL--LRPQNNTLSQKSYNKTSNIRGSSQRKATKTIFVLAIVFTTLEAPVFIIQILIQNGTFAFILNSNPHV-----VYFIINFLVVLDSCSNFMVYLVTSDKFRAEFRRLIWRSGCYSQGRQRSVINDNFSVTEQLIVTQSQKMASIIHRIRPD 1157
            M +++FSN+C+  N+T   + S+P Y  ++I  SILA+  NG+SV+ILSR N+S RFG IRLNLLILSISET+LN+ I VFN+     + +   + AL  DT +FWNI +LIC RNWIV+LIA SRCE+VFLPL+SV GL FNN VI+  S++I ++SYLLS VRLFSKKAIVC+N+ ++ L+PHL   W Y +YETYCFFI+QSVLPV++V G+S  M  SLI+ P  +L   +   N L  K       I+  SQRKAT+TIFVLAIVFT+LE PVFIIQ L Q+G    IL  N +      V+F+IN LVV DSC N  VY++TSDKFR E RRL+   G Y    Q+S      S TE LIV Q+  M   +  I+ +
Sbjct:    1 MLILNFSNSCIPSNVT-SNVNSEP-YRPLIICLSILATFLNGISVIILSRSNASHRFGPIRLNLLILSISETALNSLIAVFNIASESELDKLFSVTALLFDTFVFWNILTLICTRNWIVVLIALSRCEVVFLPLNSVRGLTFNNSVIKLCSVIILLLSYLLSFVRLFSKKAIVCSNLSKILLEPHLYKSWQYVIYETYCFFIYQSVLPVILVAGVSLAMFVSLIRGPKEELTECKSSRNQLGMKR----KTIKAFSQRKATRTIFVLAIVFTSLEGPVFIIQALTQSG---MILTGNNNAKLRRQVFFLINLLVVFDSCCNVFVYMITSDKFRGEIRRLMGVFG-YCTNGQQSFALGKLSATEHLIVNQTPHMTKFLRNIQME 373
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000016965.1 (SMESG000016965.1)

HSP 1 Score: 257.299 bits (656), Expect = 1.710e-82
Identity = 164/364 (45.05%), Postives = 233/364 (64.01%), Query Frame = 3 
Query:   90 ASKPIYSYMMILFSILASLANGVSVVILSRPNSSQRFGQIRLNLLILSISETSLNAFITVFNLTHIVNISQYSR------IGALWIDTMLFWNITSLICNRNWIVILIACSRCEIVFLPLHSVSGLKFNNKVIRNXXXXXXXXXXXXXXVRLFSKKAIVCTNIDEVKLQPHLSGQWAYSVYETYCFFIFQSVLPVLVVGGISAGMTYSLIKEPNPQLLRPQN-NTLSQKSYNKTSNIRGS-----SQRKATKTIFVLAIVFTTLEAPVFIIQILIQNGTFAFILNSNPHVVYFII----NFLVVLDSCSNFMVYLVTSDKFRAEFRRLIWR-SGCYSQGRQRSVINDNFSVTEQLIVTQSQKMA 1130
            +S  IY+ + +  + +A + NG S++IL+R NS+ RFGQI+LNLL+LS+ ET L  FIT+F++     + +Y R      IG LW+D ++F+NITSL C RNWIV++IACSRCEI+F+P +S  GLKFN+   + ++++I   SY +S VRLF+KKA++C + +  +L  HL    AY +++TYCFFI+QSVLPV++VG +S  M++SL+           + N  S    N  S+ R S     SQRKAT+TI VLA+VFT LE PVFIIQ ++    F     SN  ++   +    N LVVLDSCSN +VYL+TSDKFR E RR+ W+ S C     Q    N+  S TE L+V Q+  ++
Sbjct:    7 SSSKIYATVSMALATMAFIFNGFSLIILTRKNSNLRFGQIKLNLLLLSLVETFLTGFITLFHV-----VVEYKRSANSGGIGLLWLDLIVFYNITSLTCTRNWIVVIIACSRCEIIFVPFNSRRGLKFNHHNTKIIALIILFFSYCISFVRLFAKKALICQDTNVPQLANHLLDYRAYRLFKTYCFFIYQSVLPVVIVGIVSLAMSFSLLGSTRSSSYNSTHINDCSYFRMNHLSDQRHSSRKLNSQRKATRTILVLAVVFTLLEVPVFIIQSVVDRDDF-----SNHSILKIKLTELSNILVVLDSCSNIVVYLLTSDKFRLEVRRIFWQISSC-----QLCDENNYLSATEHLVVHQNNVLS 355
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000063918.1 (SMESG000063918.1)

HSP 1 Score: 140.198 bits (352), Expect = 9.436e-38
Identity = 111/346 (32.08%), Postives = 180/346 (52.02%), Query Frame = 3 
Query:   30 MFLISFSNNC----VFENITRPTLASKPIYSYMMILF----SILASLANGVSVVILSRPNSSQRFGQIRLNLLILSISETSLNAFITVFNLTHIVNISQYSRIGALWIDTMLFWNITSLICNRNWIVILIACSRCEIVFLPLHSVSGLKFNNKVIRNXXXXXXXXXXXXXXVRLFSKKAIVCTNIDEVKLQPHLSGQWAYSVYETYCFFIFQSVLPVLVVGGISAGMTYSLIKEPNPQLLRPQNNTLSQKSYNKTSNIRGSSQRKATKTIFVLAIVFTTLEAPVFIIQIL---IQNGTFAFILNSNPHVVYFIINFLVVLDSCSNFMVYLVTSDKFRAEFRRLIWR 1034
            MFLI +  NC     F      +     ++ Y   +F    +I+  + N ++V  LS+P    R   +R      +I E  L+ +I    ++   ++     + A+   T+ FW+I +L+C+RNW+V+L+A +R E +F PL   S   F++   R  +I++ ++S+ LS +R+F KKAIVC N +++    H      Y  +E   +FI+QSV PV++V   +  M Y L K+        ++  L ++ + +      +SQ+KAT TI VLA VFT  E P+FII +L     NG       SN  V+Y I   LVV+DSC NF++Y+ TS+ +R +   L  R
Sbjct:    1 MFLIHYVRNCSNVYYFHKNLNFSSNEYELFDYSTNIFIAVVAIIGFVTNTLTVFTLSQPRFKGRSHNMRYTSFYHAIIELVLDLWIFTDKISMFAHLPDDKYVSAMIFSTLTFWSINALLCSRNWMVMLVALARLEAIFRPLKIRSYSIFSSCKSRLYTIIVLLLSFSLSVIRVFYKKAIVCENENKIIFDSHWKNSSLYQCFEI-GYFIYQSVFPVVIVTISTLTMIYFLFKQT------FKSTHLLKQYFRENETQCMTSQQKATLTITVLAGVFTVTEIPIFIITLLKFTYPNGL------SNLMVLYQITRALVVVDSCCNFLIYVSTSEHYRRDLWYLFQR 333
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000072341.1 (SMESG000072341.1)

HSP 1 Score: 92.0485 bits (227), Expect = 2.226e-20
Identity = 101/382 (26.44%), Postives = 171/382 (44.76%), Query Frame = 3 
Query:   30 MFLISFSNNCVFENITRPTLA-SKPIYS----YMMIL---FSILASLANGVSVVILSR---PNSSQRFGQIRLNLLILSISETSLNAFITVFNLTHIVNISQYSRIGALWIDTMLFWNITSLICNRNWIVILIACSRCEIVFLPLHSVSGLKFNNKVIRNXXXXXXXXXXXXXXVRLFSKKAIVCTNIDEVKLQPHLSGQWAYSVYETY------CFFIFQSVLPVLVVGGISAGMTYSL------IKEPNPQLLRPQNNTLSQ---KSYNKTSN-----------IRGSSQRKATKTIFVLAIVFTTLEAPVFIIQILIQNGTFAFILNSNPHVVY--FIINFLVVLDSCSNFMVYLVTSDKFRAEFRRLIWRSGCYSQGR 1058
            M    +SN+C F N+T   L  S+ +Y     Y+M +    S +A + NG+S+++  R    NS+  F     NL+ +S+ E  L   +   ++  I  I +Y    A    +++   + ++   RNW V+LI+ SRCE+V  PL     L  N++ ++ +   + +IS+ LS ++    K IVC N+ E      L+ ++  S+Y  Y        F+FQS +PV+++   +  M Y L              +     TL Q   K YN+ +            I   S+   T+ + +L+ VF  L+ P FII        F  I+    ++++     N L+VL S  N  VY  +S +FR   + +I       Q R
Sbjct:    1 MLFYEYSNSC-FVNLTNFNLTDSQKLYRIQEWYLMTVILPLSFIAGIVNGLSLLVFFRQVFDNSAVHF-----NLMAISLVEVVLQIVVICESIFIITGIERYHYKTASLATSLIHITLNAMFSIRNWTVVLISVSRCEVVLRPLSLRKRLFLNSRCLKTVLGSLILISFGLSILKALRLKGIVCINLSE-----RLTRRYLLSIYPFYQHIIVHGMFLFQSCIPVILIMLATGVMWYKLHQCKAMFASEKKVKIDLAEKTLRQQKIKEYNEKTKPVELNARSRGMISSKSRNNTTQMVMILSFVFGLLQLPTFIIT------CFTMIVKHRLYLIHPDIFANCLLVLGSILNVFVYAASSKRFRLNLKSMINSKHTTYQSR 365
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
galr2a2.625e-626.90galanin receptor 2a [Source:ZFIN;Acc:ZDB-GENE-1306... [more]
galr2a3.007e-626.90galanin receptor 2a [Source:ZFIN;Acc:ZDB-GENE-1306... [more]
galr2a3.007e-626.90galanin receptor 2a [Source:ZFIN;Acc:ZDB-GENE-1306... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A183SJQ71.722e-2529.74G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A3P7M9355.153e-2330.81G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A0R3T1S67.190e-2226.36G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A0R3UKE89.471e-2228.44G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A0R3WEV82.196e-2127.43G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000078733.10.000e+099.73SMESG000078733.1[more]
SMESG000031763.16.976e-11952.74SMESG000031763.1[more]
SMESG000016965.11.710e-8245.05SMESG000016965.1[more]
SMESG000063918.19.436e-3832.08SMESG000063918.1[more]
SMESG000072341.12.226e-2026.44SMESG000072341.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011813 ID=SMED30011813|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1179bp
ATTTTCTATCATTATTCTTTCATTGAATTATGTTTTTGATTTCTTTTTCA
AATAACTGCGTCTTTGAAAATATAACTAGGCCGACTTTGGCTTCAAAACC
GATCTATTCGTACATGATGATCCTGTTTAGCATTCTCGCAAGTCTTGCTA
ATGGGGTGTCAGTTGTAATACTCTCTCGGCCAAATTCGAGTCAACGATTC
GGACAAATACGACTGAATTTGCTAATTCTATCAATTTCGGAAACCAGCCT
AAATGCATTTATAACCGTTTTTAATTTGACACATATTGTGAATATCAGCC
AATACTCTCGCATCGGAGCCCTGTGGATAGATACGATGCTGTTCTGGAAT
ATTACTTCCCTTATTTGCAATCGGAACTGGATTGTGATATTAATAGCTTG
TTCTAGGTGTGAAATCGTATTTCTCCCACTCCACAGCGTTTCTGGCCTTA
AGTTCAACAATAAAGTCATTCGAAATCTGTCTATTTTGATTTTTATCATT
TCGTACTTGCTGTCTTCGGTGAGATTGTTTTCCAAAAAGGCAATAGTTTG
TACAAACATTGATGAGGTAAAATTACAGCCCCACTTAAGCGGTCAATGGG
CCTATTCGGTTTATGAAACTTATTGCTTTTTTATATTCCAATCAGTTTTA
CCAGTACTTGTCGTTGGTGGCATCAGTGCTGGCATGACTTATAGTTTAAT
AAAAGAGCCAAATCCTCAACTACTGCGACCACAAAACAACACTCTTTCTC
AAAAATCGTACAACAAGACATCGAATATCCGTGGCTCGTCTCAACGCAAA
GCTACAAAAACTATTTTCGTATTAGCAATAGTCTTCACTACTCTAGAAGC
TCCTGTGTTTATTATTCAAATCCTAATTCAAAACGGCACATTTGCATTTA
TCTTAAATTCAAACCCCCATGTTGTGTATTTTATCATTAATTTTTTAGTT
GTTTTAGACTCTTGTTCTAACTTTATGGTTTATCTGGTGACAAGTGATAA
ATTTCGTGCCGAATTCCGACGGTTGATATGGCGTTCAGGATGTTACAGTC
AAGGTCGTCAAAGGTCGGTGATAAATGATAACTTTTCTGTAACAGAACAA
CTGATAGTTACACAGTCGCAGAAAATGGCATCAATTATTCATCGAATTCG
TCCGGATTAATTGAAATTAGAGATCGGAA
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protein sequence of SMED30011813-orf-1

>SMED30011813-orf-1 ID=SMED30011813-orf-1|Name=SMED30011813-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=123bp
MFVQTIAFLENNLTEDSKYEMIKIKIDRFRMTLLLNLRPETLWSGRNTIS
HLEQAINITIQFRLQIREVIFQNSIVSIHRAPMREYWLIFTICVKLKTVI
NAFRLVSEIDRISKFSRICPNR*
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protein sequence of SMED30011813-orf-2

>SMED30011813-orf-2 ID=SMED30011813-orf-2|Name=SMED30011813-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=377bp
MFLISFSNNCVFENITRPTLASKPIYSYMMILFSILASLANGVSVVILSR
PNSSQRFGQIRLNLLILSISETSLNAFITVFNLTHIVNISQYSRIGALWI
DTMLFWNITSLICNRNWIVILIACSRCEIVFLPLHSVSGLKFNNKVIRNL
SILIFIISYLLSSVRLFSKKAIVCTNIDEVKLQPHLSGQWAYSVYETYCF
FIFQSVLPVLVVGGISAGMTYSLIKEPNPQLLRPQNNTLSQKSYNKTSNI
RGSSQRKATKTIFVLAIVFTTLEAPVFIIQILIQNGTFAFILNSNPHVVY
FIINFLVVLDSCSNFMVYLVTSDKFRAEFRRLIWRSGCYSQGRQRSVIND
NFSVTEQLIVTQSQKMASIIHRIRPD*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000419prepharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 13..344
e-value: 8.7E-30
score: 105.7
NoneNo IPR availablePANTHERPTHR46641FAMILY NOT NAMEDcoord: 9..350
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 24..329
e-value: 4.91918E-16
score: 75.7472
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 19..346
NoneNo IPR availableTMHMMTMhelixcoord: 61..83
NoneNo IPR availableTMHMMTMhelixcoord: 146..168
NoneNo IPR availableTMHMMTMhelixcoord: 261..283
NoneNo IPR availableTMHMMTMhelixcoord: 26..48
NoneNo IPR availableTMHMMTMhelixcoord: 201..223
NoneNo IPR availableTMHMMTMhelixcoord: 298..320
NoneNo IPR availableTMHMMTMhelixcoord: 103..125
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 40..318
score: 14.133