Histone H2B

Overview


Name	Histone H2B
Smed ID	SMED30011775
Length (bp)	570

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Histone H2B (SMED30011775) t-SNE clustered cells

Violin plots show distribution of expression levels for Histone H2B (SMED30011775) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Histone H2B (SMED30011775) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Histone H2B (SMED30011775) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30011775

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
X2 cell	SMED30011775	SMESG000052758.1	SmedASXL_001712	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
neoblast	SMED30011775	SMESG000052758.1	dd_Smed_v4_13086_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30011775	SMESG000052758.1	dd_Smed_v4_13086_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of Histone H2B vs. Ensembl Human
Match: H2BC13 (H2B clustered histone 13 [Source:HGNC Symbol;Acc:HGNC:4748])

HSP 1 Score: 166.392 bits (420), Expect = 7.221e-53
Identity = 77/91 (84.62%), Postives = 86/91 (94.51%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY +Y+YKVL+QVHPDTGIS KAM IMNSFVND+FERIA+EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   36 ESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 126

[Close]

BLAST of Histone H2B vs. Ensembl Human
Match: H2BU1 (H2B.U histone 1 [Source:HGNC Symbol;Acc:HGNC:20514])

HSP 1 Score: 166.392 bits (420), Expect = 9.593e-53
Identity = 77/91 (84.62%), Postives = 86/91 (94.51%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAM IMNSFVND+FERIA+EAS+LA YNK+STI+SRE+QTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   36 ESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIASEASRLAHYNKRSTITSREVQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 126

[Close]

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAM IMNSFVND+FERIA+EAS+LA YNK+STI+SRE+QTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   36 ESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIASEASRLAHYNKRSTITSREVQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 126

[Close]

BLAST of Histone H2B vs. Ensembl Human
Match: H2BC1 (H2B clustered histone 1 [Source:HGNC Symbol;Acc:HGNC:18730])

HSP 1 Score: 166.007 bits (419), Expect = 1.218e-52
Identity = 79/91 (86.81%), Postives = 86/91 (94.51%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IYIYKVL+QVHPDTGIS KAMSIMNSFV D+FERIA+EAS+LA Y+K+STISSREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   37 ESYSIYIYKVLKQVHPDTGISSKAMSIMNSFVTDIFERIASEASRLAHYSKRSTISSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 127

[Close]

BLAST of Histone H2B vs. Ensembl Human
Match: H2BC21 (H2B clustered histone 21 [Source:HGNC Symbol;Acc:HGNC:4760])

HSP 1 Score: 165.622 bits (418), Expect = 1.827e-52
Identity = 78/91 (85.71%), Postives = 85/91 (93.41%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAM IMNSFVND+FERIA EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   36 ESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 126

[Close]

BLAST of Histone H2B vs. Ensembl Celegans
Match: his-41 (Probable histone H2B 3 [Source:UniProtKB/Swiss-Prot;Acc:Q27484])

HSP 1 Score: 169.859 bits (429), Expect = 1.090e-54
Identity = 80/91 (87.91%), Postives = 87/91 (95.60%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY +YIY+VL+QVHPDTG+S KAMSIMNSFVNDVFERIA+EAS+LA YNK+STISSREIQTAVRLILPGELAKHAVSEGTKAVTKYT SK
Sbjct:   33 ESYSVYIYRVLKQVHPDTGVSSKAMSIMNSFVNDVFERIASEASRLAHYNKRSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTSSK 123

[Close]

BLAST of Histone H2B vs. Ensembl Celegans
Match: his-66 (Probable histone H2B 4 [Source:UniProtKB/Swiss-Prot;Acc:Q27876])

HSP 1 Score: 169.859 bits (429), Expect = 1.126e-54
Identity = 80/91 (87.91%), Postives = 87/91 (95.60%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY +YIY+VL+QVHPDTG+S KAMSIMNSFVNDVFERIA+EAS+LA YNK+STISSREIQTAVRLILPGELAKHAVSEGTKAVTKYT SK
Sbjct:   33 ESYSVYIYRVLKQVHPDTGVSSKAMSIMNSFVNDVFERIASEASRLAHYNKRSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTSSK 123

[Close]

BLAST of Histone H2B vs. Ensembl Celegans
Match: his-62 (Probable histone H2B 4 [Source:UniProtKB/Swiss-Prot;Acc:Q27876])

HSP 1 Score: 169.859 bits (429), Expect = 1.126e-54
Identity = 80/91 (87.91%), Postives = 87/91 (95.60%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY +YIY+VL+QVHPDTG+S KAMSIMNSFVNDVFERIA+EAS+LA YNK+STISSREIQTAVRLILPGELAKHAVSEGTKAVTKYT SK
Sbjct:   33 ESYSVYIYRVLKQVHPDTGVSSKAMSIMNSFVNDVFERIASEASRLAHYNKRSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTSSK 123

[Close]

BLAST of Histone H2B vs. Ensembl Celegans
Match: his-58 (Probable histone H2B 4 [Source:UniProtKB/Swiss-Prot;Acc:Q27876])

HSP 1 Score: 169.859 bits (429), Expect = 1.126e-54
Identity = 80/91 (87.91%), Postives = 87/91 (95.60%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY +YIY+VL+QVHPDTG+S KAMSIMNSFVNDVFERIA+EAS+LA YNK+STISSREIQTAVRLILPGELAKHAVSEGTKAVTKYT SK
Sbjct:   33 ESYSVYIYRVLKQVHPDTGVSSKAMSIMNSFVNDVFERIASEASRLAHYNKRSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTSSK 123

[Close]

BLAST of Histone H2B vs. Ensembl Celegans
Match: his-48 (Probable histone H2B 4 [Source:UniProtKB/Swiss-Prot;Acc:Q27876])

HSP 1 Score: 169.859 bits (429), Expect = 1.126e-54
Identity = 80/91 (87.91%), Postives = 87/91 (95.60%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY +YIY+VL+QVHPDTG+S KAMSIMNSFVNDVFERIA+EAS+LA YNK+STISSREIQTAVRLILPGELAKHAVSEGTKAVTKYT SK
Sbjct:   33 ESYSVYIYRVLKQVHPDTGVSSKAMSIMNSFVNDVFERIASEASRLAHYNKRSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTSSK 123

[Close]

BLAST of Histone H2B vs. Ensembl Fly
Match: His2B:CG33888 (gene:FBgn0053888 transcript:FBtr0091892)

HSP 1 Score: 169.088 bits (427), Expect = 3.578e-54
Identity = 80/91 (87.91%), Postives = 86/91 (94.51%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IYIYKVL+QVHPDTGIS KAMSIMNSFVND+FERIA EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   33 ESYAIYIYKVLKQVHPDTGISSKAMSIMNSFVNDIFERIAAEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 123

[Close]

BLAST of Histone H2B vs. Ensembl Fly
Match: His2B:CG33904 (gene:FBgn0053904 transcript:FBtr0091908)

HSP 1 Score: 169.088 bits (427), Expect = 3.578e-54
Identity = 80/91 (87.91%), Postives = 86/91 (94.51%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IYIYKVL+QVHPDTGIS KAMSIMNSFVND+FERIA EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   33 ESYAIYIYKVLKQVHPDTGISSKAMSIMNSFVNDIFERIAAEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 123

[Close]

BLAST of Histone H2B vs. Ensembl Fly
Match: His2B:CG33900 (gene:FBgn0053900 transcript:FBtr0091904)

HSP 1 Score: 169.088 bits (427), Expect = 3.578e-54
Identity = 80/91 (87.91%), Postives = 86/91 (94.51%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IYIYKVL+QVHPDTGIS KAMSIMNSFVND+FERIA EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   33 ESYAIYIYKVLKQVHPDTGISSKAMSIMNSFVNDIFERIAAEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 123

[Close]

BLAST of Histone H2B vs. Ensembl Fly
Match: His2B:CG33892 (gene:FBgn0053892 transcript:FBtr0091896)

HSP 1 Score: 169.088 bits (427), Expect = 3.578e-54
Identity = 80/91 (87.91%), Postives = 86/91 (94.51%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IYIYKVL+QVHPDTGIS KAMSIMNSFVND+FERIA EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   33 ESYAIYIYKVLKQVHPDTGISSKAMSIMNSFVNDIFERIAAEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 123

[Close]

BLAST of Histone H2B vs. Ensembl Fly
Match: His2B:CG33908 (gene:FBgn0053908 transcript:FBtr0091912)

HSP 1 Score: 169.088 bits (427), Expect = 3.578e-54
Identity = 80/91 (87.91%), Postives = 86/91 (94.51%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IYIYKVL+QVHPDTGIS KAMSIMNSFVND+FERIA EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   33 ESYAIYIYKVLKQVHPDTGISSKAMSIMNSFVNDIFERIAAEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 123

[Close]

BLAST of Histone H2B vs. Ensembl Zebrafish
Match: si:ch211-190p8.2 (si:ch211-190p8.2 [Source:ZFIN;Acc:ZDB-GENE-070424-145])

HSP 1 Score: 167.548 bits (423), Expect = 1.608e-53
Identity = 78/91 (85.71%), Postives = 87/91 (95.60%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            E+Y +YIYKVL+QVHPDTGIS +AMSIMNSFVNDVFERIATEAS+LA YNK+STI+SRE+QTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   28 ETYAVYIYKVLKQVHPDTGISSRAMSIMNSFVNDVFERIATEASRLAHYNKRSTITSREVQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 118

[Close]

BLAST of Histone H2B vs. Ensembl Zebrafish
Match: zgc:173585 (zgc:173585 [Source:ZFIN;Acc:ZDB-GENE-070822-26])

HSP 1 Score: 166.392 bits (420), Expect = 4.971e-53
Identity = 79/91 (86.81%), Postives = 85/91 (93.41%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAM IMNSFVND+FERIA EAS+LA YNK+STISSREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   34 ESYAIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTISSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 124

[Close]

BLAST of Histone H2B vs. Ensembl Zebrafish
Match: si:dkey-108k21.11 (si:dkey-108k21.11 [Source:ZFIN;Acc:ZDB-GENE-131127-105])

HSP 1 Score: 166.392 bits (420), Expect = 5.913e-53
Identity = 79/91 (86.81%), Postives = 85/91 (93.41%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAM IMNSFVND+FERIA EAS+LA YNK+STISSREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   35 ESYAIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTISSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 125

[Close]

BLAST of Histone H2B vs. Ensembl Zebrafish
Match: si:dkey-261m9.6 (si:dkey-261m9.6 [Source:ZFIN;Acc:ZDB-GENE-131127-103])

HSP 1 Score: 166.007 bits (419), Expect = 6.532e-53
Identity = 79/91 (86.81%), Postives = 85/91 (93.41%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAM IMNSFVND+FERIA EAS+LA YNK+STISSREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   34 ESYAIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTISSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 124

[Close]

BLAST of Histone H2B vs. Ensembl Zebrafish
Match: zgc:194989 (zgc:194989 [Source:ZFIN;Acc:ZDB-GENE-080723-3])

HSP 1 Score: 166.007 bits (419), Expect = 8.216e-53
Identity = 78/91 (85.71%), Postives = 85/91 (93.41%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAM IMNSFVND+FERIA EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   34 ESYAIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 124

[Close]

BLAST of Histone H2B vs. Ensembl Xenopus
Match: h2bc11 (H2B clustered histone 11 [Source:NCBI gene;Acc:550005])

HSP 1 Score: 167.548 bits (423), Expect = 2.702e-53
Identity = 79/91 (86.81%), Postives = 86/91 (94.51%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAMSIMNSFVNDVFERIA EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT +K
Sbjct:   36 ESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDVFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK 126

[Close]

BLAST of Histone H2B vs. Ensembl Xenopus
Match: ENSXETT00000047283.1 (histone H2B 1.1-like [Source:NCBI gene;Acc:100497082])

HSP 1 Score: 167.548 bits (423), Expect = 2.702e-53
Identity = 79/91 (86.81%), Postives = 86/91 (94.51%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAMSIMNSFVNDVFERIA EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT +K
Sbjct:   36 ESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDVFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK 126

[Close]

BLAST of Histone H2B vs. Ensembl Xenopus
Match: krt15.2 (keratin 15, type I gene 2 [Source:Xenbase;Acc:XB-GENE-6040163])

HSP 1 Score: 167.548 bits (423), Expect = 2.702e-53
Identity = 79/91 (86.81%), Postives = 86/91 (94.51%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAMSIMNSFVNDVFERIA EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT +K
Sbjct:   36 ESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDVFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK 126

[Close]

BLAST of Histone H2B vs. Ensembl Xenopus
Match: tmf1 (TATA element modulatory factor 1 [Source:Xenbase;Acc:XB-GENE-958628])

HSP 1 Score: 167.548 bits (423), Expect = 2.702e-53
Identity = 79/91 (86.81%), Postives = 86/91 (94.51%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAMSIMNSFVNDVFERIA EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT +K
Sbjct:   36 ESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDVFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK 126

[Close]

BLAST of Histone H2B vs. Ensembl Xenopus
Match: hist2h2bf (histone H2B 1.1-like [Source:NCBI gene;Acc:108648862])

HSP 1 Score: 167.548 bits (423), Expect = 2.702e-53
Identity = 79/91 (86.81%), Postives = 86/91 (94.51%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAMSIMNSFVNDVFERIA EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT +K
Sbjct:   36 ESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDVFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK 126

[Close]

BLAST of Histone H2B vs. Ensembl Mouse
Match: H2bc1 (H2B clustered histone 1 [Source:MGI Symbol;Acc:MGI:2448375])

HSP 1 Score: 166.777 bits (421), Expect = 4.112e-53
Identity = 79/91 (86.81%), Postives = 86/91 (94.51%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IYIYKVL+QVHPDTGIS KAMSIMNSFV D+FERIA+EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   37 ESYSIYIYKVLKQVHPDTGISSKAMSIMNSFVTDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 127

[Close]

BLAST of Histone H2B vs. Ensembl Mouse
Match: H2bc11 (H2B clustered histone 11 [Source:MGI Symbol;Acc:MGI:2448388])

HSP 1 Score: 166.777 bits (421), Expect = 4.694e-53
Identity = 77/91 (84.62%), Postives = 86/91 (94.51%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY +Y+YKVL+QVHPDTGIS KAM IMNSFVND+FERIA+EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   36 ESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 126

[Close]

BLAST of Histone H2B vs. Ensembl Mouse
Match: Hist1h2br (histone cluster 1 H2br [Source:MGI Symbol;Acc:MGI:3710645])

HSP 1 Score: 166.777 bits (421), Expect = 4.694e-53
Identity = 77/91 (84.62%), Postives = 86/91 (94.51%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY +Y+YKVL+QVHPDTGIS KAM IMNSFVND+FERIA+EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   36 ESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 126

[Close]

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY +Y+YKVL+QVHPDTGIS KAM IMNSFVND+FERIA+EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   36 ESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 126

[Close]

BLAST of Histone H2B vs. Ensembl Mouse
Match: Hist1h2bq (histone cluster 1, H2bq [Source:MGI Symbol;Acc:MGI:3702051])

HSP 1 Score: 166.777 bits (421), Expect = 4.694e-53
Identity = 77/91 (84.62%), Postives = 86/91 (94.51%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY +Y+YKVL+QVHPDTGIS KAM IMNSFVND+FERIA+EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   36 ESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 126

[Close]

BLAST of Histone H2B vs. UniProt/SwissProt
Match: sp|P07794|H2BL1_PSAMI (Late histone H2B.2.1 OS=Psammechinus miliaris OX=7660 PE=3 SV=2)

HSP 1 Score: 171.785 bits (434), Expect = 3.237e-54
Identity = 83/91 (91.21%), Postives = 87/91 (95.60%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESYGIYIYKVL+QVHPDTGIS KAMSIMNSFVNDVFERIA EAS+LA YNKKSTISSRE+QTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   34 ESYGIYIYKVLKQVHPDTGISSKAMSIMNSFVNDVFERIAGEASRLAHYNKKSTISSREVQTAVRLLLPGELAKHAVSEGTKAVTKYTTSK 124

[Close]

BLAST of Histone H2B vs. UniProt/SwissProt
Match: sp|P16890|H2BL4_STRPU (Late histone H2B.L4 (Fragment) OS=Strongylocentrotus purpuratus OX=7668 PE=1 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 4.253e-54
Identity = 82/91 (90.11%), Postives = 87/91 (95.60%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESYGIYIYKVL+QVHPDTGIS +AMSIMNSFVNDVFERIA EAS+LA YNKKSTISSRE+QTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   17 ESYGIYIYKVLKQVHPDTGISSRAMSIMNSFVNDVFERIAGEASRLAQYNKKSTISSREVQTAVRLLLPGELAKHAVSEGTKAVTKYTTSK 107

[Close]

BLAST of Histone H2B vs. UniProt/SwissProt
Match: sp|P07795|H2BL2_PSAMI (Late histone H2B.2.2 OS=Psammechinus miliaris OX=7660 PE=3 SV=2)

HSP 1 Score: 171.014 bits (432), Expect = 4.798e-54
Identity = 82/91 (90.11%), Postives = 87/91 (95.60%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESYGIYIYKVL+QVHPDTGIS KAMSIMNSFVNDVFERIA EAS+L+ YNKKSTISSRE+QTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   34 ESYGIYIYKVLKQVHPDTGISSKAMSIMNSFVNDVFERIAGEASRLSQYNKKSTISSREVQTAVRLLLPGELAKHAVSEGTKAVTKYTTSK 124

[Close]

BLAST of Histone H2B vs. UniProt/SwissProt
Match: sp|Q27484|H2B3_CAEEL (Probable histone H2B 3 OS=Caenorhabditis elegans OX=6239 GN=his-41 PE=3 SV=3)

HSP 1 Score: 169.859 bits (429), Expect = 1.386e-53
Identity = 80/91 (87.91%), Postives = 87/91 (95.60%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY +YIY+VL+QVHPDTG+S KAMSIMNSFVNDVFERIA+EAS+LA YNK+STISSREIQTAVRLILPGELAKHAVSEGTKAVTKYT SK
Sbjct:   33 ESYSVYIYRVLKQVHPDTGVSSKAMSIMNSFVNDVFERIASEASRLAHYNKRSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTSSK 123

[Close]

BLAST of Histone H2B vs. UniProt/SwissProt
Match: sp|Q27876|H2B4_CAEEL (Probable histone H2B 4 OS=Caenorhabditis elegans OX=6239 GN=his-48 PE=3 SV=3)

HSP 1 Score: 169.859 bits (429), Expect = 1.433e-53
Identity = 80/91 (87.91%), Postives = 87/91 (95.60%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY +YIY+VL+QVHPDTG+S KAMSIMNSFVNDVFERIA+EAS+LA YNK+STISSREIQTAVRLILPGELAKHAVSEGTKAVTKYT SK
Sbjct:   33 ESYSVYIYRVLKQVHPDTGVSSKAMSIMNSFVNDVFERIASEASRLAHYNKRSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTSSK 123

[Close]

BLAST of Histone H2B vs. TrEMBL
Match: I1ZIK6 (Histone H2B (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 185.267 bits (469), Expect = 3.749e-57
Identity = 90/91 (98.90%), Postives = 91/91 (100.00%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIA+EASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK
Sbjct:   34 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIASEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 124

[Close]

BLAST of Histone H2B vs. TrEMBL
Match: S5VDL5 (Histone H2B (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 184.111 bits (466), Expect = 9.029e-57
Identity = 89/91 (97.80%), Postives = 91/91 (100.00%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIA+EASKLATYNKKSTI+SREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK
Sbjct:   29 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIASEASKLATYNKKSTITSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 119

[Close]

BLAST of Histone H2B vs. TrEMBL
Match: F8WLE6 (Histone H2B (Fragment) OS=Dugesia japonica OX=6161 GN=DjhistonH2B PE=2 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 3.181e-55
Identity = 86/91 (94.51%), Postives = 90/91 (98.90%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIA+EASKLA YNK+STISSREIQT+VRLILPGEL+KHAVSEGTKAVTKYTGSK
Sbjct:   22 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIASEASKLAAYNKRSTISSREIQTSVRLILPGELSKHAVSEGTKAVTKYTGSK 112

[Close]

BLAST of Histone H2B vs. TrEMBL
Match: K1QY71 (Histone H2B OS=Crassostrea gigas OX=29159 GN=CGI_10022468 PE=3 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 1.176e-52
Identity = 83/88 (94.32%), Postives = 86/88 (97.73%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYT 426
            ESYGIYIYKVLRQVHPDTGIS KAMSIMNSFVNDVFERIA EAS+LATYNK+STISSREIQTAVRL+LPGELAKHAVSEGTKAVTKYT
Sbjct:   34 ESYGIYIYKVLRQVHPDTGISSKAMSIMNSFVNDVFERIAAEASRLATYNKRSTISSREIQTAVRLLLPGELAKHAVSEGTKAVTKYT 121

[Close]

BLAST of Histone H2B vs. TrEMBL
Match: A0A0R3W3H9 (Histone H2B OS=Taenia asiatica OX=60517 GN=TASK_LOCUS4475 PE=3 SV=1)

HSP 1 Score: 172.94 bits (437), Expect = 2.348e-52
Identity = 82/91 (90.11%), Postives = 87/91 (95.60%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IYIYKVLRQVHPDTGIS KAMSIMNSFVND+FERIA E+S+LA YNKKSTI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYTGSK
Sbjct:   32 ESYAIYIYKVLRQVHPDTGISSKAMSIMNSFVNDIFERIAAESSRLAHYNKKSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTGSK 122

[Close]

BLAST of Histone H2B vs. Ensembl Cavefish
Match: si:ch211-190p8.2 (late histone H2B.L4-like [Source:NCBI gene;Acc:103022800])

HSP 1 Score: 165.622 bits (418), Expect = 6.097e-53
Identity = 76/91 (83.52%), Postives = 86/91 (94.51%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            E+Y +YIYKVL+QVHPDTGIS +AMSIMNSFVNDVF+RIATEAS+L  YNK+STI+SRE+QTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   27 ETYAVYIYKVLKQVHPDTGISSRAMSIMNSFVNDVFDRIATEASRLTQYNKRSTITSREVQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 117

[Close]

BLAST of Histone H2B vs. Ensembl Cavefish
Match: hist2h2l (histone 2, H2, like [Source:NCBI gene;Acc:386920])

HSP 1 Score: 166.007 bits (419), Expect = 1.823e-52
Identity = 78/91 (85.71%), Postives = 85/91 (93.41%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAM IMNSFVND+FERIA EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   36 ESYAIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 126

[Close]

BLAST of Histone H2B vs. Ensembl Cavefish
Match: ENSAMXT00000025763.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000081.1:1681215:1683989:-1 gene:ENSAMXG00000033961.1 transcript:ENSAMXT00000025763.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 164.081 bits (414), Expect = 2.753e-52
Identity = 77/91 (84.62%), Postives = 85/91 (93.41%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAM IMNSFVND+FERIA E+S+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   27 ESYAIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGESSRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 117

[Close]

BLAST of Histone H2B vs. Ensembl Cavefish
Match: ENSAMXT00000018389.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002305.1:122420:122920:1 gene:ENSAMXG00000040093.1 transcript:ENSAMXT00000018389.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 163.696 bits (413), Expect = 4.268e-52
Identity = 77/91 (84.62%), Postives = 85/91 (93.41%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAM IMNSFVND+FERIA E+S+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   26 ESYAIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGESSRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 116

[Close]

BLAST of Histone H2B vs. Ensembl Cavefish
Match: ENSAMXT00000026773.2 (pep primary_assembly:Astyanax_mexicanus-2.0:21:10103882:10104382:1 gene:ENSAMXG00000043772.1 transcript:ENSAMXT00000026773.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 163.696 bits (413), Expect = 4.268e-52
Identity = 77/91 (84.62%), Postives = 85/91 (93.41%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAM IMNSFVND+FERIA E+S+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   26 ESYAIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGESSRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 116

[Close]

BLAST of Histone H2B vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002374.1 (pep scaffold:Pmarinus_7.0:GL476925:48107:49207:1 gene:ENSPMAG00000002162.1 transcript:ENSPMAT00000002374.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 169.474 bits (428), Expect = 7.739e-55
Identity = 80/97 (82.47%), Postives = 89/97 (91.75%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK*MLLHI 453
            ESY IYIYKV++QVHPDTGIS KAMSIMNSFVND+FERIA+EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK    H+
Sbjct:   43 ESYAIYIYKVMKQVHPDTGISSKAMSIMNSFVNDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSKYPCDHL 139

[Close]

BLAST of Histone H2B vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002397.1 (pep scaffold:Pmarinus_7.0:GL476925:238292:239398:-1 gene:ENSPMAG00000002183.1 transcript:ENSPMAT00000002397.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 169.474 bits (428), Expect = 7.739e-55
Identity = 80/97 (82.47%), Postives = 89/97 (91.75%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK*MLLHI 453
            ESY IYIYKV++QVHPDTGIS KAMSIMNSFVND+FERIA+EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK    H+
Sbjct:   43 ESYAIYIYKVMKQVHPDTGISSKAMSIMNSFVNDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSKYPCDHL 139

[Close]

BLAST of Histone H2B vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005327.1 (pep scaffold:Pmarinus_7.0:GL476820:705043:705550:-1 gene:ENSPMAG00000004856.1 transcript:ENSPMAT00000005327.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 169.474 bits (428), Expect = 1.007e-54
Identity = 79/91 (86.81%), Postives = 87/91 (95.60%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IYIYKV++QVHPDTGIS KAMSIMNSFVND+FERIA+EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   43 ESYAIYIYKVMKQVHPDTGISSKAMSIMNSFVNDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 133

[Close]

BLAST of Histone H2B vs. Ensembl Sea Lamprey
Match: ENSPMAT00000011164.1 (pep scaffold:Pmarinus_7.0:GL476820:1014653:1015054:1 gene:ENSPMAG00000010137.1 transcript:ENSPMAT00000011164.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 169.088 bits (427), Expect = 1.027e-54
Identity = 79/91 (86.81%), Postives = 87/91 (95.60%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IYIYKV++QVHPDTGIS KAMSIMNSFVND+FERIA+EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   43 ESYAIYIYKVMKQVHPDTGISSKAMSIMNSFVNDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 133

[Close]

BLAST of Histone H2B vs. Ensembl Sea Lamprey
Match: ENSPMAT00000011062.1 (pep scaffold:Pmarinus_7.0:GL476925:226546:226947:1 gene:ENSPMAG00000010034.1 transcript:ENSPMAT00000011062.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 169.088 bits (427), Expect = 1.027e-54
Identity = 79/91 (86.81%), Postives = 87/91 (95.60%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IYIYKV++QVHPDTGIS KAMSIMNSFVND+FERIA+EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   43 ESYAIYIYKVMKQVHPDTGISSKAMSIMNSFVNDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 133

[Close]

BLAST of Histone H2B vs. Ensembl Yeast
Match: HTB1 (Histone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation [Source:SGD;Acc:S000002632])

HSP 1 Score: 163.696 bits (413), Expect = 6.605e-53
Identity = 76/90 (84.44%), Postives = 84/90 (93.33%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGS 432
            E+Y  YIYKVL+Q HPDTGIS K+MSI+NSFVND+FERIATEASKLA YNKKSTIS+REIQTAVRLILPGELAKHAVSEGT+AVTKY+ S
Sbjct:   39 ETYSSYIYKVLKQTHPDTGISQKSMSILNSFVNDIFERIATEASKLAAYNKKSTISAREIQTAVRLILPGELAKHAVSEGTRAVTKYSSS 128

[Close]

BLAST of Histone H2B vs. Ensembl Yeast
Match: HTB2 (Histone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation [Source:SGD;Acc:S000000098])

HSP 1 Score: 163.696 bits (413), Expect = 8.773e-53
Identity = 76/90 (84.44%), Postives = 84/90 (93.33%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGS 432
            E+Y  YIYKVL+Q HPDTGIS K+MSI+NSFVND+FERIATEASKLA YNKKSTIS+REIQTAVRLILPGELAKHAVSEGT+AVTKY+ S
Sbjct:   39 ETYSSYIYKVLKQTHPDTGISQKSMSILNSFVNDIFERIATEASKLAAYNKKSTISAREIQTAVRLILPGELAKHAVSEGTRAVTKYSSS 128

[Close]

BLAST of Histone H2B vs. Ensembl Nematostella
Match: EDO34626 (Histone H2B [Source:UniProtKB/TrEMBL;Acc:A7SNU9])

HSP 1 Score: 166.007 bits (419), Expect = 1.655e-53
Identity = 80/91 (87.91%), Postives = 84/91 (92.31%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IYIYKVLRQVHPDTGIS KAM IMNSFVND+FERIA EAS+LA YNKK TISSRE+QTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   28 ESYAIYIYKVLRQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKKHTISSREVQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 118

[Close]

BLAST of Histone H2B vs. Ensembl Nematostella
Match: EDO46280 (Histone H2B [Source:UniProtKB/TrEMBL;Acc:A7RQF6])

HSP 1 Score: 166.007 bits (419), Expect = 1.655e-53
Identity = 80/91 (87.91%), Postives = 84/91 (92.31%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IYIYKVLRQVHPDTGIS KAM IMNSFVND+FERIA EAS+LA YNKK TISSRE+QTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   28 ESYAIYIYKVLRQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKKHTISSREVQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 118

[Close]

BLAST of Histone H2B vs. Ensembl Nematostella
Match: EDO30783 (Histone H2B [Source:UniProtKB/TrEMBL;Acc:A7SZS7])

HSP 1 Score: 166.007 bits (419), Expect = 1.720e-53
Identity = 79/91 (86.81%), Postives = 85/91 (93.41%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IYIYKVL+QVHPDTGIS KAM IMNSFVND+FERIA E+S+LA YNKKSTISSREIQTA+RL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   32 ESYAIYIYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAAESSRLAHYNKKSTISSREIQTAIRLLLPGELAKHAVSEGTKAVTKYTSSK 122

[Close]

BLAST of Histone H2B vs. Ensembl Nematostella
Match: EDO26787 (Histone H2B [Source:UniProtKB/TrEMBL;Acc:A7SZS7])

HSP 1 Score: 166.007 bits (419), Expect = 1.720e-53
Identity = 79/91 (86.81%), Postives = 85/91 (93.41%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IYIYKVL+QVHPDTGIS KAM IMNSFVND+FERIA E+S+LA YNKKSTISSREIQTA+RL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   32 ESYAIYIYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAAESSRLAHYNKKSTISSREIQTAIRLLLPGELAKHAVSEGTKAVTKYTSSK 122

[Close]

BLAST of Histone H2B vs. Ensembl Nematostella
Match: EDO26931 (Histone H2B [Source:UniProtKB/TrEMBL;Acc:A7SZS7])

HSP 1 Score: 166.007 bits (419), Expect = 1.720e-53
Identity = 79/91 (86.81%), Postives = 85/91 (93.41%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IYIYKVL+QVHPDTGIS KAM IMNSFVND+FERIA E+S+LA YNKKSTISSREIQTA+RL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   32 ESYAIYIYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAAESSRLAHYNKKSTISSREIQTAIRLLLPGELAKHAVSEGTKAVTKYTSSK 122

[Close]

BLAST of Histone H2B vs. Ensembl Medaka
Match: ENSORLT00000036814.1 (histone H2B 1/2 [Source:NCBI gene;Acc:101159064])

HSP 1 Score: 169.088 bits (427), Expect = 3.997e-54
Identity = 79/91 (86.81%), Postives = 87/91 (95.60%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAMSIMNSFVND+FERIA+EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   34 ESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 124

[Close]

BLAST of Histone H2B vs. Ensembl Medaka
Match: ENSORLT00000034562.1 (histone H2B 1/2 [Source:NCBI gene;Acc:101167975])

HSP 1 Score: 169.088 bits (427), Expect = 3.997e-54
Identity = 79/91 (86.81%), Postives = 87/91 (95.60%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAMSIMNSFVND+FERIA+EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   34 ESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 124

[Close]

BLAST of Histone H2B vs. Ensembl Medaka
Match: ENSORLT00000044844.1 (histone H2B 1/2 [Source:NCBI gene;Acc:111947380])

HSP 1 Score: 169.088 bits (427), Expect = 3.997e-54
Identity = 79/91 (86.81%), Postives = 87/91 (95.60%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAMSIMNSFVND+FERIA+EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   34 ESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 124

[Close]

BLAST of Histone H2B vs. Ensembl Medaka
Match: ENSORLT00000030260.1 (histone H2B 1/2 [Source:NCBI gene;Acc:101172789])

HSP 1 Score: 169.088 bits (427), Expect = 3.997e-54
Identity = 79/91 (86.81%), Postives = 87/91 (95.60%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAMSIMNSFVND+FERIA+EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:   34 ESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 124

[Close]

BLAST of Histone H2B vs. Ensembl Medaka
Match: ENSORLT00000032861.1 (pep primary_assembly:ASM223467v1:1:1206779:1243811:-1 gene:ENSORLG00000023088.1 transcript:ENSORLT00000032861.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 169.088 bits (427), Expect = 3.179e-53
Identity = 79/91 (86.81%), Postives = 87/91 (95.60%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESY IY+YKVL+QVHPDTGIS KAMSIMNSFVND+FERIA+EAS+LA YNK+STI+SREIQTAVRL+LPGELAKHAVSEGTKAVTKYT SK
Sbjct:  109 ESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 199

HSP 2 Score: 117.472 bits (293), Expect = 5.988e-33
Identity = 53/63 (84.13%), Postives = 60/63 (95.24%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTA 351
            ESY IY+YKVL+QVHPDTGIS KAMSIMNSFVND+FERIA+EAS+LA YNK+STI+SREIQTA
Sbjct:   34 ESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDIFERIASEASRLAHYNKRSTITSREIQTA 96

[Close]

BLAST of Histone H2B vs. Planmine SMEST
Match: SMESG000052758.1 (SMESG000052758.1)

HSP 1 Score: 187.578 bits (475), Expect = 1.250e-61
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK
Sbjct:   33 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 123

[Close]

BLAST of Histone H2B vs. Planmine SMEST
Match: SMESG000052748.1 (SMESG000052748.1)

HSP 1 Score: 187.578 bits (475), Expect = 1.441e-61
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK
Sbjct:   33 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 123

[Close]

BLAST of Histone H2B vs. Planmine SMEST
Match: SMESG000069642.1 (SMESG000069642.1)

HSP 1 Score: 185.267 bits (469), Expect = 9.986e-61
Identity = 89/91 (97.80%), Postives = 91/91 (100.00%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ES+GIYIYKVLRQVHPDTG+SGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK
Sbjct:   33 ESFGIYIYKVLRQVHPDTGVSGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 123

[Close]

BLAST of Histone H2B vs. Planmine SMEST
Match: SMESG000067899.1 (SMESG000067899.1)

HSP 1 Score: 185.267 bits (469), Expect = 1.043e-60
Identity = 90/91 (98.90%), Postives = 91/91 (100.00%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIA+EASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK
Sbjct:   34 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIASEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 124

[Close]

BLAST of Histone H2B vs. Planmine SMEST
Match: SMESG000067906.1 (SMESG000067906.1)

HSP 1 Score: 184.496 bits (467), Expect = 1.966e-60
Identity = 89/91 (97.80%), Postives = 91/91 (100.00%), Query Frame = 1

Query:  163 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 435
            ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIA+EASKLATYNKKSTI+SREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK
Sbjct:   34 ESYGIYIYKVLRQVHPDTGISGKAMSIMNSFVNDVFERIASEASKLATYNKKSTITSREIQTAVRLILPGELAKHAVSEGTKAVTKYTGSK 124

The following BLAST results are available for this feature:

BLAST of Histone H2B vs. Ensembl Human
BLAST of Histone H2B vs. Ensembl Celegans
BLAST of Histone H2B vs. Ensembl Fly
BLAST of Histone H2B vs. Ensembl Zebrafish
BLAST of Histone H2B vs. Ensembl Xenopus
BLAST of Histone H2B vs. Ensembl Mouse
BLAST of Histone H2B vs. UniProt/SwissProt
BLAST of Histone H2B vs. TrEMBL
BLAST of Histone H2B vs. Ensembl Cavefish
BLAST of Histone H2B vs. Ensembl Sea Lamprey
BLAST of Histone H2B vs. Ensembl Yeast
BLAST of Histone H2B vs. Ensembl Nematostella
BLAST of Histone H2B vs. Ensembl Medaka
BLAST of Histone H2B vs. Planmine SMEST

BLAST of Histone H2B vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
H2BC13	7.221e-53	84.62	H2B clustered histone 13 [Source:HGNC Symbol;Acc:H...	[more]
H2BU1	9.593e-53	84.62	H2B.U histone 1 [Source:HGNC Symbol;Acc:HGNC:20514...	[more]
H2BU1	9.593e-53	84.62	H2B.U histone 1 [Source:HGNC Symbol;Acc:HGNC:20514...	[more]
H2BC1	1.218e-52	86.81	H2B clustered histone 1 [Source:HGNC Symbol;Acc:HG...	[more]
H2BC21	1.827e-52	85.71	H2B clustered histone 21 [Source:HGNC Symbol;Acc:H...	[more]

BLAST of Histone H2B vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
his-41	1.090e-54	87.91	Probable histone H2B 3 [Source:UniProtKB/Swiss-Pr...	[more]
his-66	1.126e-54	87.91	Probable histone H2B 4 [Source:UniProtKB/Swiss-Pr...	[more]
his-62	1.126e-54	87.91	Probable histone H2B 4 [Source:UniProtKB/Swiss-Pr...	[more]
his-58	1.126e-54	87.91	Probable histone H2B 4 [Source:UniProtKB/Swiss-Pr...	[more]
his-48	1.126e-54	87.91	Probable histone H2B 4 [Source:UniProtKB/Swiss-Pr...	[more]

BLAST of Histone H2B vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
His2B:CG33888	3.578e-54	87.91	gene:FBgn0053888 transcript:FBtr0091892	[more]
His2B:CG33904	3.578e-54	87.91	gene:FBgn0053904 transcript:FBtr0091908	[more]
His2B:CG33900	3.578e-54	87.91	gene:FBgn0053900 transcript:FBtr0091904	[more]
His2B:CG33892	3.578e-54	87.91	gene:FBgn0053892 transcript:FBtr0091896	[more]
His2B:CG33908	3.578e-54	87.91	gene:FBgn0053908 transcript:FBtr0091912	[more]

BLAST of Histone H2B vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
si:ch211-190p8.2	1.608e-53	85.71	si:ch211-190p8.2 [Source:ZFIN;Acc:ZDB-GENE-070424-...	[more]
zgc:173585	4.971e-53	86.81	zgc:173585 [Source:ZFIN;Acc:ZDB-GENE-070822-26]	[more]
si:dkey-108k21.11	5.913e-53	86.81	si:dkey-108k21.11 [Source:ZFIN;Acc:ZDB-GENE-131127...	[more]
si:dkey-261m9.6	6.532e-53	86.81	si:dkey-261m9.6 [Source:ZFIN;Acc:ZDB-GENE-131127-1...	[more]
zgc:194989	8.216e-53	85.71	zgc:194989 [Source:ZFIN;Acc:ZDB-GENE-080723-3]	[more]

BLAST of Histone H2B vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
h2bc11	2.702e-53	86.81	H2B clustered histone 11 [Source:NCBI gene;Acc:550...	[more]
ENSXETT00000047283.1	2.702e-53	86.81	histone H2B 1.1-like [Source:NCBI gene;Acc:1004970...	[more]
krt15.2	2.702e-53	86.81	keratin 15, type I gene 2 [Source:Xenbase;Acc:XB-G...	[more]
tmf1	2.702e-53	86.81	TATA element modulatory factor 1 [Source:Xenbase;A...	[more]
hist2h2bf	2.702e-53	86.81	histone H2B 1.1-like [Source:NCBI gene;Acc:1086488...	[more]

BLAST of Histone H2B vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
H2bc1	4.112e-53	86.81	H2B clustered histone 1 [Source:MGI Symbol;Acc:MGI...	[more]
H2bc11	4.694e-53	84.62	H2B clustered histone 11 [Source:MGI Symbol;Acc:MG...	[more]
Hist1h2br	4.694e-53	84.62	histone cluster 1 H2br [Source:MGI Symbol;Acc:MGI:...	[more]
Hist1h2br	4.694e-53	84.62	histone cluster 1 H2br [Source:MGI Symbol;Acc:MGI:...	[more]
Hist1h2bq	4.694e-53	84.62	histone cluster 1, H2bq [Source:MGI Symbol;Acc:MGI...	[more]

BLAST of Histone H2B vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P07794\|H2BL1_PSAMI	3.237e-54	91.21	Late histone H2B.2.1 OS=Psammechinus miliaris OX=7...	[more]
sp\|P16890\|H2BL4_STRPU	4.253e-54	90.11	Late histone H2B.L4 (Fragment) OS=Strongylocentrot...	[more]
sp\|P07795\|H2BL2_PSAMI	4.798e-54	90.11	Late histone H2B.2.2 OS=Psammechinus miliaris OX=7...	[more]
sp\|Q27484\|H2B3_CAEEL	1.386e-53	87.91	Probable histone H2B 3 OS=Caenorhabditis elegans O...	[more]
sp\|Q27876\|H2B4_CAEEL	1.433e-53	87.91	Probable histone H2B 4 OS=Caenorhabditis elegans O...	[more]

BLAST of Histone H2B vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
I1ZIK6	3.749e-57	98.90	Histone H2B (Fragment) OS=Schmidtea mediterranea O...	[more]
S5VDL5	9.029e-57	97.80	Histone H2B (Fragment) OS=Schmidtea mediterranea O...	[more]
F8WLE6	3.181e-55	94.51	Histone H2B (Fragment) OS=Dugesia japonica OX=6161...	[more]
K1QY71	1.176e-52	94.32	Histone H2B OS=Crassostrea gigas OX=29159 GN=CGI_1...	[more]
A0A0R3W3H9	2.348e-52	90.11	Histone H2B OS=Taenia asiatica OX=60517 GN=TASK_LO...	[more]

BLAST of Histone H2B vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
si:ch211-190p8.2	6.097e-53	83.52	late histone H2B.L4-like [Source:NCBI gene;Acc:103...	[more]
hist2h2l	1.823e-52	85.71	histone 2, H2, like [Source:NCBI gene;Acc:386920]	[more]
ENSAMXT00000025763.2	2.753e-52	84.62	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]
ENSAMXT00000018389.2	4.268e-52	84.62	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]
ENSAMXT00000026773.2	4.268e-52	84.62	pep primary_assembly:Astyanax_mexicanus-2.0:21:101...	[more]

BLAST of Histone H2B vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSPMAT00000002374.1	7.739e-55	82.47	pep scaffold:Pmarinus_7.0:GL476925:48107:49207:1 g...	[more]
ENSPMAT00000002397.1	7.739e-55	82.47	pep scaffold:Pmarinus_7.0:GL476925:238292:239398:-...	[more]
ENSPMAT00000005327.1	1.007e-54	86.81	pep scaffold:Pmarinus_7.0:GL476820:705043:705550:-...	[more]
ENSPMAT00000011164.1	1.027e-54	86.81	pep scaffold:Pmarinus_7.0:GL476820:1014653:1015054...	[more]
ENSPMAT00000011062.1	1.027e-54	86.81	pep scaffold:Pmarinus_7.0:GL476925:226546:226947:1...	[more]

BLAST of Histone H2B vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2


Match Name	E-value	Identity	Description
HTB1	6.605e-53	84.44	Histone H2B; core histone protein required for chr...	[more]
HTB2	8.773e-53	84.44	Histone H2B; core histone protein required for chr...	[more]

BLAST of Histone H2B vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO34626	1.655e-53	87.91	Histone H2B [Source:UniProtKB/TrEMBL;Acc:A7SNU9]	[more]
EDO46280	1.655e-53	87.91	Histone H2B [Source:UniProtKB/TrEMBL;Acc:A7RQF6]	[more]
EDO30783	1.720e-53	86.81	Histone H2B [Source:UniProtKB/TrEMBL;Acc:A7SZS7]	[more]
EDO26787	1.720e-53	86.81	Histone H2B [Source:UniProtKB/TrEMBL;Acc:A7SZS7]	[more]
EDO26931	1.720e-53	86.81	Histone H2B [Source:UniProtKB/TrEMBL;Acc:A7SZS7]	[more]

BLAST of Histone H2B vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSORLT00000036814.1	3.997e-54	86.81	histone H2B 1/2 [Source:NCBI gene;Acc:101159064]	[more]
ENSORLT00000034562.1	3.997e-54	86.81	histone H2B 1/2 [Source:NCBI gene;Acc:101167975]	[more]
ENSORLT00000044844.1	3.997e-54	86.81	histone H2B 1/2 [Source:NCBI gene;Acc:111947380]	[more]
ENSORLT00000030260.1	3.997e-54	86.81	histone H2B 1/2 [Source:NCBI gene;Acc:101172789]	[more]
ENSORLT00000032861.1	3.179e-53	86.81	pep primary_assembly:ASM223467v1:1:1206779:1243811...	[more]

BLAST of Histone H2B vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000052758.1	1.250e-61	100.00	SMESG000052758.1	[more]
SMESG000052748.1	1.441e-61	100.00	SMESG000052748.1	[more]
SMESG000069642.1	9.986e-61	97.80	SMESG000069642.1	[more]
SMESG000067899.1	1.043e-60	98.90	SMESG000067899.1	[more]
SMESG000067906.1	1.966e-60	97.80	SMESG000067906.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30011775 ID=SMED30011775|Name=Histone H2B|organism=Schmidtea mediterranea sexual|type=transcript|length=570bp
CTTAACTAAAAGTTAACCATATAAATCGAATTCGAAATTTTTAAGATATA
TTAATTATTATCGAAAATGGCTCCAAAAGCAAAAGTTGCTGCTAAATCAG
TGAAGAAAATTTCAAAGGAAGTTGTCAAGTCTGACAAAAAGAAAAGAACT
CACAAACGTAAAGAAAGCTATGGTATCTATATCTACAAGGTTCTCAGACA
AGTTCATCCAGACACTGGAATTTCTGGTAAAGCCATGTCAATAATGAACA
GTTTCGTTAATGATGTGTTCGAAAGAATTGCAACTGAAGCTAGCAAGTTG
GCAACTTACAACAAGAAGTCAACTATTTCTAGCAGAGAGATTCAAACTGC
AGTTAGATTGATCTTGCCTGGAGAATTGGCTAAGCACGCTGTGAGCGAAG
GAACCAAAGCTGTTACCAAATATACTGGTTCAAAGTAAATGCTATTACAT
ATATAAGTCTGAGAATGTTAAACGGTCCTCTTTAGGACCACAAATTATGA
TTGTAAATAGTAGACATTTTTAAATAACAGATTTTGGTATCTGTAATATT
AGCCTTGGTACCACGCACAA

protein sequence of SMED30011775-orf-1

>SMED30011775-orf-1 ID=SMED30011775-orf-1|Name=SMED30011775-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=124bp
MAPKAKVAAKSVKKISKEVVKSDKKKRTHKRKESYGIYIYKVLRQVHPDT
GISGKAMSIMNSFVNDVFERIATEASKLATYNKKSTISSREIQTAVRLIL
PGELAKHAVSEGTKAVTKYTGSK*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0002111	X2 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR000558	Histone_H2B
IPR007125	Histone_H2A/H2B/H3
IPR009072	Histone-fold

Vocabulary: **cellular component**
Term	Definition
GO:0000786	nucleosome
GO:0005634	nucleus
GO:0005694	chromosome

Vocabulary: **molecular function**
Term	Definition
GO:0003677	DNA binding
GO:0046982	protein heterodimerization activity

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000558	Histone H2B	PRINTS	PR00621	HISTONEH2B	coord: 93..106 score: 91.63 coord: 35..53 score: 81.25 coord: 54..74 score: 78.68 coord: 106..119 score: 95.85 coord: 76..93 score: 75.56
IPR000558	Histone H2B	SMART	SM00427	h2b3	coord: 25..121 e-value: 6.5E-74 score: 261.5
IPR000558	Histone H2B	PANTHER	PTHR23428	HISTONE H2B	coord: 9..123
IPR000558	Histone H2B	PROSITE	PS00357	HISTONE_H2B	coord: 90..112
IPR007125	Histone H2A/H2B/H3	PFAM	PF00125	Histone	coord: 10..98 e-value: 1.2E-21 score: 77.4
IPR009072	Histone-fold	GENE3D	G3DSA:1.10.20.10		coord: 1..123 e-value: 2.9E-60 score: 203.8
IPR009072	Histone-fold	SUPERFAMILY	SSF47113	Histone-fold	coord: 9..123
None	No IPR available	PANTHER	PTHR23428:SF252		coord: 9..123