smad4
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30011742 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of smad4 vs. Ensembl Human
Match: SMAD4 (SMAD family member 4 [Source:HGNC Symbol;Acc:HGNC:6770]) HSP 1 Score: 307.76 bits (787), Expect = 8.191e-97 Identity = 156/233 (66.95%), Postives = 185/233 (79.40%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 224 PEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQP 454 HSP 2 Score: 183.341 bits (464), Expect = 3.611e-50 Identity = 104/191 (54.45%), Postives = 139/191 (72.77%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSSIEDLDDTESPSVMDSESVNETLKYISNSSNGPDDSLHMRARMTLQYGSSN 646
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D+S L+L S+ +PS M + +Y+ + P S + T+Q+ SN
Sbjct: 11 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSN--------APSSMMVKD-----EYVHDFEGQPSLSTEGHSIQTIQHPPSN 188
BLAST of smad4 vs. Ensembl Human
Match: SMAD4 (SMAD family member 4 [Source:HGNC Symbol;Acc:HGNC:6770]) HSP 1 Score: 307.375 bits (786), Expect = 2.029e-95 Identity = 156/233 (66.95%), Postives = 185/233 (79.40%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 320 PEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQP 550 HSP 2 Score: 183.341 bits (464), Expect = 2.026e-49 Identity = 104/191 (54.45%), Postives = 139/191 (72.77%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSSIEDLDDTESPSVMDSESVNETLKYISNSSNGPDDSLHMRARMTLQYGSSN 646
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D+S L+L S+ +PS M + +Y+ + P S + T+Q+ SN
Sbjct: 11 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSN--------APSSMMVKD-----EYVHDFEGQPSLSTEGHSIQTIQHPPSN 188
BLAST of smad4 vs. Ensembl Human
Match: SMAD4 (SMAD family member 4 [Source:HGNC Symbol;Acc:HGNC:6770]) HSP 1 Score: 307.375 bits (786), Expect = 2.029e-95 Identity = 156/233 (66.95%), Postives = 185/233 (79.40%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 320 PEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQP 550 HSP 2 Score: 183.341 bits (464), Expect = 2.026e-49 Identity = 104/191 (54.45%), Postives = 139/191 (72.77%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSSIEDLDDTESPSVMDSESVNETLKYISNSSNGPDDSLHMRARMTLQYGSSN 646
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D+S L+L S+ +PS M + +Y+ + P S + T+Q+ SN
Sbjct: 11 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSN--------APSSMMVKD-----EYVHDFEGQPSLSTEGHSIQTIQHPPSN 188
BLAST of smad4 vs. Ensembl Human
Match: SMAD4 (SMAD family member 4 [Source:HGNC Symbol;Acc:HGNC:6770]) HSP 1 Score: 214.157 bits (544), Expect = 2.488e-64 Identity = 96/126 (76.19%), Postives = 110/126 (87.30%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQ 1465
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+
Sbjct: 54 PEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHR 179
BLAST of smad4 vs. Ensembl Human
Match: SMAD4 (SMAD family member 4 [Source:HGNC Symbol;Acc:HGNC:6770]) HSP 1 Score: 184.882 bits (468), Expect = 3.617e-52 Identity = 104/190 (54.74%), Postives = 139/190 (73.16%), Query Frame = 2
Query: 77 SNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSSIEDLDDTESPSVMDSESVNETLKYISNSSNGPDDSLHMRARMTLQYGSSN 646
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D+S L+L S+ +PS M + +Y+ + P S + T+Q+ SN
Sbjct: 12 SNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSN--------APSSMMVKD-----EYVHDFEGQPSLSTEGHSIQTIQHPPSN 188
BLAST of smad4 vs. Ensembl Celegans
Match: sma-4 (Dwarfin sma-4 [Source:UniProtKB/Swiss-Prot;Acc:P45897]) HSP 1 Score: 157.147 bits (396), Expect = 1.400e-40 Identity = 105/269 (39.03%), Postives = 150/269 (55.76%), Query Frame = 2
Query: 1013 NQNQGF--DRP----PTTSAQP--LQALTHQRPPEY---WCTIAYFELNQQVGELFKVPSQ-YSCVTVDGYTDPSSPN--RFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPG-AYIKVFDIRQCHQQM-KKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLS-PFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMS 1768
N N GF D+P P S P L + PP+ WC+I Y+EL+ +GE FKV ++ + V VDG DP N R CLG LSNVHR+E+SEK+R++IG+GVEL +G++ I S +FV+S YLD G + H+ P + VFDIR + QM +++ + + + AAV + S+ ++ GVD + FC + +SFVK WG Y R IKETPCWIE+ L+RPLQ+LD++L++ S
Sbjct: 306 NFNGGFAPDQPQLVTPIISDIPIDLNQIYVPTPPQLLDNWCSIIYYELDTPIGETFKVSARDHGKVIVDGGMDPHGENEGRLCLGALSNVHRTEASEKARIHIGRGVELTAHADGNISI--TSNCKIFVRSGYLDYTHGSEYSSKAHRFTPNESSFTVFDIRWAYMQMLRRSRSSNEAVRAQAAAVAGYAPMSVMPAIMPDS------------GVDRMRRDFCTIAISFVKAWGDVYQRKTIKETPCWIEVTLHRPLQILDQLLKNSS 560 HSP 2 Score: 135.191 bits (339), Expect = 3.673e-33 Identity = 80/135 (59.26%), Postives = 102/135 (75.56%), Query Frame = 2
Query: 83 DACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSL 487
D+C I + L C++QGGE +F RKAIESLVKKLK+KR ELD LITAVT++G QPTGCVTI ++LDGRLQ+AGRK PHV+Y+RIWRWP + KNEL + C + D +CINPYHY+RVV + + ++ SL
Sbjct: 141 DSCQQISHVLQCYQQGGEDSDFVRKAIESLVKKLKDKRIELDALITAVTSNGKQPTGCVTIQRSLDGRLQVAGRKGVPHVVYARIWRWPKVSKNELVKLVQCQTSSDHP-DNICINPYHYERVVSNRITSADQSL 274
BLAST of smad4 vs. Ensembl Celegans
Match: sma-4 (Dwarfin sma-4 [Source:UniProtKB/Swiss-Prot;Acc:P45897]) HSP 1 Score: 157.147 bits (396), Expect = 1.400e-40 Identity = 105/269 (39.03%), Postives = 150/269 (55.76%), Query Frame = 2
Query: 1013 NQNQGF--DRP----PTTSAQP--LQALTHQRPPEY---WCTIAYFELNQQVGELFKVPSQ-YSCVTVDGYTDPSSPN--RFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPG-AYIKVFDIRQCHQQM-KKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLS-PFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMS 1768
N N GF D+P P S P L + PP+ WC+I Y+EL+ +GE FKV ++ + V VDG DP N R CLG LSNVHR+E+SEK+R++IG+GVEL +G++ I S +FV+S YLD G + H+ P + VFDIR + QM +++ + + + AAV + S+ ++ GVD + FC + +SFVK WG Y R IKETPCWIE+ L+RPLQ+LD++L++ S
Sbjct: 306 NFNGGFAPDQPQLVTPIISDIPIDLNQIYVPTPPQLLDNWCSIIYYELDTPIGETFKVSARDHGKVIVDGGMDPHGENEGRLCLGALSNVHRTEASEKARIHIGRGVELTAHADGNISI--TSNCKIFVRSGYLDYTHGSEYSSKAHRFTPNESSFTVFDIRWAYMQMLRRSRSSNEAVRAQAAAVAGYAPMSVMPAIMPDS------------GVDRMRRDFCTIAISFVKAWGDVYQRKTIKETPCWIEVTLHRPLQILDQLLKNSS 560 HSP 2 Score: 135.191 bits (339), Expect = 3.673e-33 Identity = 80/135 (59.26%), Postives = 102/135 (75.56%), Query Frame = 2
Query: 83 DACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSL 487
D+C I + L C++QGGE +F RKAIESLVKKLK+KR ELD LITAVT++G QPTGCVTI ++LDGRLQ+AGRK PHV+Y+RIWRWP + KNEL + C + D +CINPYHY+RVV + + ++ SL
Sbjct: 141 DSCQQISHVLQCYQQGGEDSDFVRKAIESLVKKLKDKRIELDALITAVTSNGKQPTGCVTIQRSLDGRLQVAGRKGVPHVVYARIWRWPKVSKNELVKLVQCQTSSDHP-DNICINPYHYERVVSNRITSADQSL 274
BLAST of smad4 vs. Ensembl Celegans
Match: sma-4 (Dwarfin sma-4 [Source:UniProtKB/Swiss-Prot;Acc:P45897]) HSP 1 Score: 157.147 bits (396), Expect = 1.400e-40 Identity = 105/269 (39.03%), Postives = 150/269 (55.76%), Query Frame = 2
Query: 1013 NQNQGF--DRP----PTTSAQP--LQALTHQRPPEY---WCTIAYFELNQQVGELFKVPSQ-YSCVTVDGYTDPSSPN--RFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPG-AYIKVFDIRQCHQQM-KKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLS-PFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMS 1768
N N GF D+P P S P L + PP+ WC+I Y+EL+ +GE FKV ++ + V VDG DP N R CLG LSNVHR+E+SEK+R++IG+GVEL +G++ I S +FV+S YLD G + H+ P + VFDIR + QM +++ + + + AAV + S+ ++ GVD + FC + +SFVK WG Y R IKETPCWIE+ L+RPLQ+LD++L++ S
Sbjct: 306 NFNGGFAPDQPQLVTPIISDIPIDLNQIYVPTPPQLLDNWCSIIYYELDTPIGETFKVSARDHGKVIVDGGMDPHGENEGRLCLGALSNVHRTEASEKARIHIGRGVELTAHADGNISI--TSNCKIFVRSGYLDYTHGSEYSSKAHRFTPNESSFTVFDIRWAYMQMLRRSRSSNEAVRAQAAAVAGYAPMSVMPAIMPDS------------GVDRMRRDFCTIAISFVKAWGDVYQRKTIKETPCWIEVTLHRPLQILDQLLKNSS 560 HSP 2 Score: 135.191 bits (339), Expect = 3.673e-33 Identity = 80/135 (59.26%), Postives = 102/135 (75.56%), Query Frame = 2
Query: 83 DACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSL 487
D+C I + L C++QGGE +F RKAIESLVKKLK+KR ELD LITAVT++G QPTGCVTI ++LDGRLQ+AGRK PHV+Y+RIWRWP + KNEL + C + D +CINPYHY+RVV + + ++ SL
Sbjct: 141 DSCQQISHVLQCYQQGGEDSDFVRKAIESLVKKLKDKRIELDALITAVTSNGKQPTGCVTIQRSLDGRLQVAGRKGVPHVVYARIWRWPKVSKNELVKLVQCQTSSDHP-DNICINPYHYERVVSNRITSADQSL 274
BLAST of smad4 vs. Ensembl Celegans
Match: sma-3 (Dwarfin sma-3 [Source:UniProtKB/Swiss-Prot;Acc:P45896]) HSP 1 Score: 140.584 bits (353), Expect = 5.472e-36 Identity = 83/236 (35.17%), Postives = 128/236 (54.24%), Query Frame = 2
Query: 1073 THQRPPEY-----WCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPN-RFCLGQLSNVHRSESSEKSRLYIGKGVELNNV-GEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQ 1759
HQRPP + W I YFELN +VGE+FK+ + +TVDGYT+PS+ N R CLGQL+NV+R+ + E +R++IGKG++L+N + + I S VFVQS + P V +I P + + VF+ Q ++++ ++ G+++LS C +R+SFVKGWG DYPR ++ TPCW+E++LN PL +D+ ++
Sbjct: 184 VHQRPPPFRHPKSWAQITYFELNSRVGEVFKLVNL--SITVDGYTNPSNSNTRICLGQLTNVNRNGTIENTRMHIGKGIQLDNKEDQMHIMITNNSDMPVFVQSKNTNLMMN-MPLVKVCRIPPHSQLCVFEFNLFFQMLEQSCNDSD-------------------------------------GLNELSKHCFIRISFVKGWGEDYPRQDVTSTPCWLELRLNVPLAYIDQKMK 379 HSP 2 Score: 84.3445 bits (207), Expect = 3.801e-17 Identity = 38/69 (55.07%), Postives = 49/69 (71.01%), Query Frame = 2
Query: 254 CVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHK-NELRQNKCCIYGYE--MKLDYVCINPYHYDRV 451
C+TI K+LDGRLQ++ +K PHVIY R+WRWP++ +ELR C Y YE K Y+CINPYHY R+
Sbjct: 65 CITIAKSLDGRLQVSHKKGLPHVIYCRVWRWPDISSPHELRSIDTCSYPYESSSKTMYICINPYHYQRL 133
BLAST of smad4 vs. Ensembl Celegans
Match: sma-4 (Dwarfin sma-4 [Source:UniProtKB/Swiss-Prot;Acc:P45897]) HSP 1 Score: 128.257 bits (321), Expect = 7.644e-34 Identity = 78/197 (39.59%), Postives = 113/197 (57.36%), Query Frame = 2
Query: 1193 DPSSPN--RFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPG-AYIKVFDIRQCHQQM-KKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLS-PFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMS 1768
DP N R CLG LSNVHR+E+SEK+R++IG+GVEL +G++ I S +FV+S YLD G + H+ P + VFDIR + QM +++ + + + AAV + S+ ++ GVD + FC + +SFVK WG Y R IKETPCWIE+ L+RPLQ+LD++L++ S
Sbjct: 2 DPHGENEGRLCLGALSNVHRTEASEKARIHIGRGVELTAHADGNISI--TSNCKIFVRSGYLDYTHGSEYSSKAHRFTPNESSFTVFDIRWAYMQMLRRSRSSNEAVRAQAAAVAGYAPMSVMPAIMPDS------------GVDRMRRDFCTIAISFVKAWGDVYQRKTIKETPCWIEVTLHRPLQILDQLLKNSS 184
BLAST of smad4 vs. Ensembl Fly
Match: Med (gene:FBgn0011655 transcript:FBtr0085816) HSP 1 Score: 315.849 bits (808), Expect = 1.967e-97 Identity = 158/235 (67.23%), Postives = 182/235 (77.45%), Query Frame = 2
Query: 1070 LTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVN 1774
L+ Q PPEYWC+IAYFEL+ QVGE FKVPS V +DGY DPS NRFCLG LSNVHR+E SE++RL+IGKGV+L+ GEGDVW+RCLS +SVFVQSYYLDREAGR PGDAVHKIYP A IKVFD+RQCHQQM +T AQ A QAA VAG +N G + +AAGIGVDDL C+LRLSFVKGWGPDYPR +IKETPCWIE+ L+R LQLLDEVL +M ++
Sbjct: 463 LSRQPPPEYWCSIAYFELDTQVGETFKVPSAKPNVIIDGYVDPSGGNRFCLGALSNVHRTEQSERARLHIGKGVQLDLRGEGDVWLRCLSDNSVFVQSYYLDREAGRTPGDAVHKIYPAACIKVFDLRQCHQQMHSLATNAQAAAAAQAAAVAGVANQ------QMGGGGRSMTAAAGIGVDDLRRLCILRLSFVKGWGPDYPRQSIKETPCWIEVHLHRALQLLDEVLHAMPID 691 HSP 2 Score: 183.341 bits (464), Expect = 7.110e-49 Identity = 100/156 (64.10%), Postives = 124/156 (79.49%), Query Frame = 2
Query: 62 PSPVN---SNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSSIEDLDDTE 520
P P N S DAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEKR+ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+RIWRWP+LHKNEL+ K C + +++K D VC+NPYHY+RVV +D+S LSL S L E
Sbjct: 32 PPPSNAPTSADACLSIVHSLMCHRQGGESEGFAKRAIESLVKKLKEKRDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARIWRWPDLHKNELKHVKYCAFAFDLKCDSVCVNPYHYERVVSPGIDLSGLSLQSGPSRLVKDE 187
BLAST of smad4 vs. Ensembl Fly
Match: Med (gene:FBgn0011655 transcript:FBtr0085815) HSP 1 Score: 315.849 bits (808), Expect = 1.111e-96 Identity = 158/235 (67.23%), Postives = 182/235 (77.45%), Query Frame = 2
Query: 1070 LTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVN 1774
L+ Q PPEYWC+IAYFEL+ QVGE FKVPS V +DGY DPS NRFCLG LSNVHR+E SE++RL+IGKGV+L+ GEGDVW+RCLS +SVFVQSYYLDREAGR PGDAVHKIYP A IKVFD+RQCHQQM +T AQ A QAA VAG +N G + +AAGIGVDDL C+LRLSFVKGWGPDYPR +IKETPCWIE+ L+R LQLLDEVL +M ++
Sbjct: 537 LSRQPPPEYWCSIAYFELDTQVGETFKVPSAKPNVIIDGYVDPSGGNRFCLGALSNVHRTEQSERARLHIGKGVQLDLRGEGDVWLRCLSDNSVFVQSYYLDREAGRTPGDAVHKIYPAACIKVFDLRQCHQQMHSLATNAQAAAAAQAAAVAGVANQ------QMGGGGRSMTAAAGIGVDDLRRLCILRLSFVKGWGPDYPRQSIKETPCWIEVHLHRALQLLDEVLHAMPID 765 HSP 2 Score: 183.726 bits (465), Expect = 1.312e-48 Identity = 100/156 (64.10%), Postives = 124/156 (79.49%), Query Frame = 2
Query: 62 PSPVN---SNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSSIEDLDDTE 520
P P N S DAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEKR+ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+RIWRWP+LHKNEL+ K C + +++K D VC+NPYHY+RVV +D+S LSL S L E
Sbjct: 32 PPPSNAPTSADACLSIVHSLMCHRQGGESEGFAKRAIESLVKKLKEKRDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARIWRWPDLHKNELKHVKYCAFAFDLKCDSVCVNPYHYERVVSPGIDLSGLSLQSGPSRLVKDE 187
BLAST of smad4 vs. Ensembl Fly
Match: Med (gene:FBgn0011655 transcript:FBtr0337030) HSP 1 Score: 315.849 bits (808), Expect = 1.111e-96 Identity = 158/235 (67.23%), Postives = 182/235 (77.45%), Query Frame = 2
Query: 1070 LTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVN 1774
L+ Q PPEYWC+IAYFEL+ QVGE FKVPS V +DGY DPS NRFCLG LSNVHR+E SE++RL+IGKGV+L+ GEGDVW+RCLS +SVFVQSYYLDREAGR PGDAVHKIYP A IKVFD+RQCHQQM +T AQ A QAA VAG +N G + +AAGIGVDDL C+LRLSFVKGWGPDYPR +IKETPCWIE+ L+R LQLLDEVL +M ++
Sbjct: 537 LSRQPPPEYWCSIAYFELDTQVGETFKVPSAKPNVIIDGYVDPSGGNRFCLGALSNVHRTEQSERARLHIGKGVQLDLRGEGDVWLRCLSDNSVFVQSYYLDREAGRTPGDAVHKIYPAACIKVFDLRQCHQQMHSLATNAQAAAAAQAAAVAGVANQ------QMGGGGRSMTAAAGIGVDDLRRLCILRLSFVKGWGPDYPRQSIKETPCWIEVHLHRALQLLDEVLHAMPID 765 HSP 2 Score: 183.726 bits (465), Expect = 1.312e-48 Identity = 100/156 (64.10%), Postives = 124/156 (79.49%), Query Frame = 2
Query: 62 PSPVN---SNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSSIEDLDDTE 520
P P N S DAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEKR+ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+RIWRWP+LHKNEL+ K C + +++K D VC+NPYHY+RVV +D+S LSL S L E
Sbjct: 32 PPPSNAPTSADACLSIVHSLMCHRQGGESEGFAKRAIESLVKKLKEKRDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARIWRWPDLHKNELKHVKYCAFAFDLKCDSVCVNPYHYERVVSPGIDLSGLSLQSGPSRLVKDE 187
BLAST of smad4 vs. Ensembl Fly
Match: Smox (gene:FBgn0025800 transcript:FBtr0340159) HSP 1 Score: 161.384 bits (407), Expect = 1.695e-42 Identity = 101/251 (40.24%), Postives = 138/251 (54.98%), Query Frame = 2
Query: 1013 NQNQGFDRPPTTSAQPLQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSM 1765
N N PP +A + H+ P +WC+I+Y+ELN +VGE F SQ S +TVDG+TDPS+ RFCLG LSNV+R+E E++R +IGKGV L +G G+V+ CLS S+FVQS ++ G P V KI PG +K+F+ ++ AA+++ S +S V L+ C +R+SFVKGWG +Y R + TPCWIE+ LN PLQ LD VL M
Sbjct: 270 NMNMSRLTPPADAAP---VMYHE--PAFWCSISYYELNTRVGETFHA-SQPS-ITVDGFTDPSNSERFCLGLLSNVNRNEVVEQTRRHIGKGVRLYYIG-GEVFAECLSDSSIFVQSPNCNQRYGWHPA-TVCKIPPGCNLKIFNNQEF------------------AALLSQS------------------VSQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDRVLTQM 475 HSP 2 Score: 73.559 bits (179), Expect = 2.257e-13 Identity = 46/123 (37.40%), Postives = 74/123 (60.16%), Query Frame = 2
Query: 98 IVNSLLCHRQGGESQN----FSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLH-KNELRQNKCCIYGYEMKLDYVCINPYHYDRV 451
+V LL ++G E + +S KA+++LVKK+K+ + + A++T Q T CVT+P++ RK PHVIY R+WRWP+L +NEL+ C Y + ++ + +CINPYHY ++
Sbjct: 8 VVKRLLALKKGNEDNSVEGKWSEKAVKNLVKKIKKNSQLEELE-RAISTQNCQ-TRCVTVPRSKPAPAGEHLRKGLPHVIYCRLWRWPDLQSQNELKPLDHCEYAFHLRKEEICINPYHYKKI 128
BLAST of smad4 vs. Ensembl Fly
Match: Smox (gene:FBgn0025800 transcript:FBtr0071192) HSP 1 Score: 161.384 bits (407), Expect = 1.695e-42 Identity = 101/251 (40.24%), Postives = 138/251 (54.98%), Query Frame = 2
Query: 1013 NQNQGFDRPPTTSAQPLQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSM 1765
N N PP +A + H+ P +WC+I+Y+ELN +VGE F SQ S +TVDG+TDPS+ RFCLG LSNV+R+E E++R +IGKGV L +G G+V+ CLS S+FVQS ++ G P V KI PG +K+F+ ++ AA+++ S +S V L+ C +R+SFVKGWG +Y R + TPCWIE+ LN PLQ LD VL M
Sbjct: 270 NMNMSRLTPPADAAP---VMYHE--PAFWCSISYYELNTRVGETFHA-SQPS-ITVDGFTDPSNSERFCLGLLSNVNRNEVVEQTRRHIGKGVRLYYIG-GEVFAECLSDSSIFVQSPNCNQRYGWHPA-TVCKIPPGCNLKIFNNQEF------------------AALLSQS------------------VSQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDRVLTQM 475 HSP 2 Score: 73.559 bits (179), Expect = 2.257e-13 Identity = 46/123 (37.40%), Postives = 74/123 (60.16%), Query Frame = 2
Query: 98 IVNSLLCHRQGGESQN----FSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLH-KNELRQNKCCIYGYEMKLDYVCINPYHYDRV 451
+V LL ++G E + +S KA+++LVKK+K+ + + A++T Q T CVT+P++ RK PHVIY R+WRWP+L +NEL+ C Y + ++ + +CINPYHY ++
Sbjct: 8 VVKRLLALKKGNEDNSVEGKWSEKAVKNLVKKIKKNSQLEELE-RAISTQNCQ-TRCVTVPRSKPAPAGEHLRKGLPHVIYCRLWRWPDLQSQNELKPLDHCEYAFHLRKEEICINPYHYKKI 128
BLAST of smad4 vs. Ensembl Zebrafish
Match: smad4a (SMAD family member 4a [Source:ZFIN;Acc:ZDB-GENE-990603-8]) HSP 1 Score: 306.22 bits (783), Expect = 9.304e-96 Identity = 156/233 (66.95%), Postives = 184/233 (78.97%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + P
Sbjct: 273 PEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPTP 503 HSP 2 Score: 182.57 bits (462), Expect = 1.239e-49 Identity = 99/172 (57.56%), Postives = 136/172 (79.07%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSS------IEDLDDTES-PSVMDSESVNETLKY 568
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C + +++K D VC+NPYHY+RVV +D+S L+L+ S ++D D + PS+ +E +T+++
Sbjct: 8 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQFAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLSGSGPSGLMVKDEYDYDGPPSLPSTEGHMQTIQH 179
BLAST of smad4 vs. Ensembl Zebrafish
Match: smad4a (SMAD family member 4a [Source:ZFIN;Acc:ZDB-GENE-990603-8]) HSP 1 Score: 306.605 bits (784), Expect = 2.359e-95 Identity = 156/233 (66.95%), Postives = 184/233 (78.97%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + P
Sbjct: 315 PEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPTP 545 HSP 2 Score: 182.956 bits (463), Expect = 1.574e-49 Identity = 98/172 (56.98%), Postives = 134/172 (77.91%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSS-------IEDLDDTESPSVMDSESVNETLKY 568
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C + +++K D VC+NPYHY+RVV +D+S L+L+ S ++ D PS+ +E +T+++
Sbjct: 8 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQFAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLSGSGPSGLMVKDEYDYDGPPSLPSTEGHMQTIQH 179
BLAST of smad4 vs. Ensembl Zebrafish
Match: si:dkey-222b8.1 (si:dkey-222b8.1 [Source:ZFIN;Acc:ZDB-GENE-060503-248]) HSP 1 Score: 301.597 bits (771), Expect = 2.930e-93 Identity = 158/264 (59.85%), Postives = 198/264 (75.00%), Query Frame = 2
Query: 998 SSSSWNQNQGFDRPPTTSAQPLQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKPT 1789
+S W+Q+ PT+ QP+ ++ PE+WC+I+YFE++ QVGE+FKV + VTVDGY DPS +RFCLGQLSNVHR+++SE++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYPGA IKVFD+RQCH+QM++ + AQ A QAA VAGS S + SL+ +AAGIGVDDL C+LRLSFVKGWGPDYPR IK+TPCW+E+ L+R LQLLDEVL +M + + P
Sbjct: 313 TSHFWSQHHT----PTSYPQPV---SNHPGPEFWCSISYFEMDVQVGEMFKVLANCPVVTVDGYVDPSGGDRFCLGQLSNVHRTDASERARLHIGKGVQLECRGEGDVWMRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPGALIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGSIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRLSFVKGWGPDYPRQTIKQTPCWVEVHLHRALQLLDEVLHTMPLADPGPA 567 HSP 2 Score: 179.104 bits (453), Expect = 5.069e-48 Identity = 103/169 (60.95%), Postives = 129/169 (76.33%), Query Frame = 2
Query: 47 MSLNDPSPVNSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVV-PSAVDIS-----ELSLTSSIEDLDDTESPSVM 535
MS+N PS SNDAC+SIV+SL+CHRQGGE+++F+++AIESLVKKLKEKR+ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV P V S + I D E PS M
Sbjct: 1 MSVNAPS---SNDACLSIVHSLMCHRQGGENESFAKRAIESLVKKLKEKRDELDSLITAITTNGVHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKFCQYAFDLKYDNVCVNPYHYERVVSPGIVGFSLPNTGRIIKEEHIHDCIQLEGPSSM 166
BLAST of smad4 vs. Ensembl Zebrafish
Match: si:dkey-222b8.1 (si:dkey-222b8.1 [Source:ZFIN;Acc:ZDB-GENE-060503-248]) HSP 1 Score: 301.597 bits (771), Expect = 2.930e-93 Identity = 158/264 (59.85%), Postives = 198/264 (75.00%), Query Frame = 2
Query: 998 SSSSWNQNQGFDRPPTTSAQPLQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKPT 1789
+S W+Q+ PT+ QP+ ++ PE+WC+I+YFE++ QVGE+FKV + VTVDGY DPS +RFCLGQLSNVHR+++SE++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYPGA IKVFD+RQCH+QM++ + AQ A QAA VAGS S + SL+ +AAGIGVDDL C+LRLSFVKGWGPDYPR IK+TPCW+E+ L+R LQLLDEVL +M + + P
Sbjct: 313 TSHFWSQHHT----PTSYPQPV---SNHPGPEFWCSISYFEMDVQVGEMFKVLANCPVVTVDGYVDPSGGDRFCLGQLSNVHRTDASERARLHIGKGVQLECRGEGDVWMRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPGALIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGSIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRLSFVKGWGPDYPRQTIKQTPCWVEVHLHRALQLLDEVLHTMPLADPGPA 567 HSP 2 Score: 179.104 bits (453), Expect = 5.069e-48 Identity = 103/169 (60.95%), Postives = 129/169 (76.33%), Query Frame = 2
Query: 47 MSLNDPSPVNSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVV-PSAVDIS-----ELSLTSSIEDLDDTESPSVM 535
MS+N PS SNDAC+SIV+SL+CHRQGGE+++F+++AIESLVKKLKEKR+ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV P V S + I D E PS M
Sbjct: 1 MSVNAPS---SNDACLSIVHSLMCHRQGGENESFAKRAIESLVKKLKEKRDELDSLITAITTNGVHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKFCQYAFDLKYDNVCVNPYHYERVVSPGIVGFSLPNTGRIIKEEHIHDCIQLEGPSSM 166
BLAST of smad4 vs. Ensembl Zebrafish
Match: si:dkey-222b8.1 (si:dkey-222b8.1 [Source:ZFIN;Acc:ZDB-GENE-060503-248]) HSP 1 Score: 298.516 bits (763), Expect = 4.612e-92 Identity = 150/234 (64.10%), Postives = 185/234 (79.06%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKPT 1789
PE+WC+I+YFE++ QVGE+FKV S VT+DGY DPS +RFCLGQLSNVHR+++SE++RL+IGKGV+L GEGDVW+RC+S H+VFVQSYYLDREAGR PGDAVHKIYPGAYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LRLSFVKGWGPDYPR +IK TPCW+E+ L+R LQLLDEVL +M + + P
Sbjct: 339 PEFWCSISYFEMDVQVGEMFKVLSSCPVVTIDGYVDPSGGDRFCLGQLSNVHRTDASERARLHIGKGVQLECRGEGDVWMRCMSDHAVFVQSYYLDREAGRAPGDAVHKIYPGAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAVSLS--AAAGIGVDDLRRLCILRLSFVKGWGPDYPRQSIKHTPCWVEVHLHRALQLLDEVLHTMPLADPGPA 570 HSP 2 Score: 177.563 bits (449), Expect = 2.234e-47 Identity = 96/144 (66.67%), Postives = 122/144 (84.72%), Query Frame = 2
Query: 47 MSLNDPSPVNSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVV-PSAVDIS 475
MS+N P NSNDAC+SIV+SL+CHRQGGE++ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV P V +S
Sbjct: 1 MSVNPP---NSNDACLSIVHSLMCHRQGGENEGFAKRAIESLVKKLKEKKDELDSLITAITTNGVHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKFCQYAFDLKYDNVCVNPYHYERVVSPGIVGLS 141
BLAST of smad4 vs. Ensembl Xenopus
Match: acsl6 (acyl-CoA synthetase long chain family member 6 [Source:Xenbase;Acc:XB-GENE-5834989]) HSP 1 Score: 308.145 bits (788), Expect = 5.853e-97 Identity = 160/250 (64.00%), Postives = 193/250 (77.20%), Query Frame = 2
Query: 1037 PPTTSAQPLQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
P + S+ P+ + T PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 213 PTSFSSIPVPSTT----PEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQP 456 HSP 2 Score: 183.726 bits (465), Expect = 2.336e-50 Identity = 95/141 (67.38%), Postives = 121/141 (85.82%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSS 496
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D+S L+L SS
Sbjct: 11 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSS 151
BLAST of smad4 vs. Ensembl Xenopus
Match: acsl6 (acyl-CoA synthetase long chain family member 6 [Source:Xenbase;Acc:XB-GENE-5834989]) HSP 1 Score: 308.531 bits (789), Expect = 1.992e-96 Identity = 164/265 (61.89%), Postives = 196/265 (73.96%), Query Frame = 2
Query: 1013 NQNQGFDRPPTTSAQPLQ-ALTHQRP------PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
QN +P T +P+ L Q P PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 247 TQNGFTGQPATYHHRPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQP 509 HSP 2 Score: 184.111 bits (466), Expect = 5.559e-50 Identity = 95/141 (67.38%), Postives = 121/141 (85.82%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSS 496
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D+S L+L SS
Sbjct: 8 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSS 148
BLAST of smad4 vs. Ensembl Xenopus
Match: acsl6 (acyl-CoA synthetase long chain family member 6 [Source:Xenbase;Acc:XB-GENE-5834989]) HSP 1 Score: 307.375 bits (786), Expect = 5.412e-96 Identity = 156/233 (66.95%), Postives = 185/233 (79.40%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 278 PEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQP 508 HSP 2 Score: 183.726 bits (465), Expect = 7.346e-50 Identity = 95/141 (67.38%), Postives = 121/141 (85.82%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSS 496
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D+S L+L SS
Sbjct: 8 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSS 148
BLAST of smad4 vs. Ensembl Xenopus
Match: acsl6 (acyl-CoA synthetase long chain family member 6 [Source:Xenbase;Acc:XB-GENE-5834989]) HSP 1 Score: 307.76 bits (787), Expect = 1.192e-95 Identity = 156/233 (66.95%), Postives = 185/233 (79.40%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 320 PEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQP 550 HSP 2 Score: 184.111 bits (466), Expect = 1.179e-49 Identity = 95/141 (67.38%), Postives = 121/141 (85.82%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSS 496
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D+S L+L SS
Sbjct: 11 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSS 151
BLAST of smad4 vs. Ensembl Xenopus
Match: acsl6 (acyl-CoA synthetase long chain family member 6 [Source:Xenbase;Acc:XB-GENE-5834989]) HSP 1 Score: 215.698 bits (548), Expect = 7.366e-61 Identity = 96/126 (76.19%), Postives = 110/126 (87.30%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQ 1465
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+
Sbjct: 317 PEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHR 442 HSP 2 Score: 182.956 bits (463), Expect = 4.782e-49 Identity = 95/141 (67.38%), Postives = 121/141 (85.82%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSS 496
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D+S L+L SS
Sbjct: 8 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSS 148
BLAST of smad4 vs. Ensembl Mouse
Match: Smad4 (SMAD family member 4 [Source:MGI Symbol;Acc:MGI:894293]) HSP 1 Score: 307.375 bits (786), Expect = 1.074e-95 Identity = 156/233 (66.95%), Postives = 185/233 (79.40%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 319 PEYWCSIAYFEMDVQVGETFKVPSSCPVVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQP 549 HSP 2 Score: 182.57 bits (462), Expect = 2.544e-49 Identity = 94/145 (64.83%), Postives = 122/145 (84.14%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSSIEDL 508
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D+S L+L S+ +
Sbjct: 11 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSM 155
BLAST of smad4 vs. Ensembl Mouse
Match: Smad4 (SMAD family member 4 [Source:MGI Symbol;Acc:MGI:894293]) HSP 1 Score: 307.375 bits (786), Expect = 1.074e-95 Identity = 156/233 (66.95%), Postives = 185/233 (79.40%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 319 PEYWCSIAYFEMDVQVGETFKVPSSCPVVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQP 549 HSP 2 Score: 182.57 bits (462), Expect = 2.544e-49 Identity = 94/145 (64.83%), Postives = 122/145 (84.14%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSSIEDL 508
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D+S L+L S+ +
Sbjct: 11 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSM 155
BLAST of smad4 vs. Ensembl Mouse
Match: Smad2 (SMAD family member 2 [Source:MGI Symbol;Acc:MGI:108051]) HSP 1 Score: 164.466 bits (415), Expect = 8.857e-44 Identity = 101/242 (41.74%), Postives = 139/242 (57.44%), Query Frame = 2
Query: 1040 PTTSAQPLQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSM 1765
P + LQ +T+ P +WC+IAY+ELNQ+VGE F SQ S +TVDG+TDPS+ RFCLG LSNV+R+ + E +R +IG+GV L +G G+V+ CLS ++FVQS ++ G P V KI PG +K+F+ ++ AA++A S NQG + V L+ C +R+SFVKGWG +Y R + TPCWIE+ LN PLQ LD+VL M
Sbjct: 226 PVNHSLDLQPVTYSEP-AFWCSIAYYELNQRVGETFHA-SQPS-LTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIG-GEVFAECLSDSAIFVQSPNCNQRYGWHPA-TVCKIPPGCNLKIFNNQEF------------------AALLAQS--------VNQGFEA----------VYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQM 426 HSP 2 Score: 99.7525 bits (247), Expect = 1.078e-21 Identity = 48/103 (46.60%), Postives = 66/103 (64.08%), Query Frame = 2
Query: 221 AVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHK-NELRQNKCCIYGYEMKLDYVCINPYHYDR----VVPSAVDISELSLTSSIEDLDD 514
A+TT T CVTIP++LDGRLQ++ RK PHVIY R+WRWP+LH +EL+ + C Y + +K D VC+NPYHY R V+P + + + + LDD
Sbjct: 64 AITTQNCN-TKCVTIPRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDD 165
BLAST of smad4 vs. Ensembl Mouse
Match: Smad2 (SMAD family member 2 [Source:MGI Symbol;Acc:MGI:108051]) HSP 1 Score: 164.851 bits (416), Expect = 1.199e-43 Identity = 101/242 (41.74%), Postives = 139/242 (57.44%), Query Frame = 2
Query: 1040 PTTSAQPLQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSM 1765
P + LQ +T+ P +WC+IAY+ELNQ+VGE F SQ S +TVDG+TDPS+ RFCLG LSNV+R+ + E +R +IG+GV L +G G+V+ CLS ++FVQS ++ G P V KI PG +K+F+ ++ AA++A S NQG + V L+ C +R+SFVKGWG +Y R + TPCWIE+ LN PLQ LD+VL M
Sbjct: 256 PVNHSLDLQPVTYSEP-AFWCSIAYYELNQRVGETFHA-SQPS-LTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIG-GEVFAECLSDSAIFVQSPNCNQRYGWHPA-TVCKIPPGCNLKIFNNQEF------------------AALLAQS--------VNQGFEA----------VYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQM 456 HSP 2 Score: 86.6557 bits (213), Expect = 2.482e-17 Identity = 39/87 (44.83%), Postives = 56/87 (64.37%), Query Frame = 2
Query: 269 KTLDGRLQIAGRKCFPHVIYSRIWRWPNLHK-NELRQNKCCIYGYEMKLDYVCINPYHYDR----VVPSAVDISELSLTSSIEDLDD 514
++LDGRLQ++ RK PHVIY R+WRWP+LH +EL+ + C Y + +K D VC+NPYHY R V+P + + + + LDD
Sbjct: 109 RSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDD 195
BLAST of smad4 vs. Ensembl Mouse
Match: Smad2 (SMAD family member 2 [Source:MGI Symbol;Acc:MGI:108051]) HSP 1 Score: 164.851 bits (416), Expect = 1.199e-43 Identity = 101/242 (41.74%), Postives = 139/242 (57.44%), Query Frame = 2
Query: 1040 PTTSAQPLQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSM 1765
P + LQ +T+ P +WC+IAY+ELNQ+VGE F SQ S +TVDG+TDPS+ RFCLG LSNV+R+ + E +R +IG+GV L +G G+V+ CLS ++FVQS ++ G P V KI PG +K+F+ ++ AA++A S NQG + V L+ C +R+SFVKGWG +Y R + TPCWIE+ LN PLQ LD+VL M
Sbjct: 256 PVNHSLDLQPVTYSEP-AFWCSIAYYELNQRVGETFHA-SQPS-LTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIG-GEVFAECLSDSAIFVQSPNCNQRYGWHPA-TVCKIPPGCNLKIFNNQEF------------------AALLAQS--------VNQGFEA----------VYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQM 456 HSP 2 Score: 86.6557 bits (213), Expect = 2.482e-17 Identity = 39/87 (44.83%), Postives = 56/87 (64.37%), Query Frame = 2
Query: 269 KTLDGRLQIAGRKCFPHVIYSRIWRWPNLHK-NELRQNKCCIYGYEMKLDYVCINPYHYDR----VVPSAVDISELSLTSSIEDLDD 514
++LDGRLQ++ RK PHVIY R+WRWP+LH +EL+ + C Y + +K D VC+NPYHY R V+P + + + + LDD
Sbjct: 109 RSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDD 195
BLAST of smad4 vs. UniProt/SwissProt
Match: sp|Q1HE26|SMAD4_BOVIN (Mothers against decapentaplegic homolog 4 OS=Bos taurus OX=9913 GN=SMAD4 PE=2 SV=1) HSP 1 Score: 307.76 bits (787), Expect = 6.845e-95 Identity = 160/251 (63.75%), Postives = 190/251 (75.70%), Query Frame = 2
Query: 1037 PPTTSAQPLQALTHQRP-PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PP + Q P PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 303 PPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQP 551 HSP 2 Score: 183.341 bits (464), Expect = 1.136e-48 Identity = 103/191 (53.93%), Postives = 139/191 (72.77%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSSIEDLDDTESPSVMDSESVNETLKYISNSSNGPDDSLHMRARMTLQYGSSN 646
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D+S L+L S+ PS++ + +Y+ + P S + T+Q+ SN
Sbjct: 11 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSN-------APPSMLVKD------EYVHDFEGQPSLSTEGHSIQTIQHPPSN 188
BLAST of smad4 vs. UniProt/SwissProt
Match: sp|O70437|SMAD4_RAT (Mothers against decapentaplegic homolog 4 OS=Rattus norvegicus OX=10116 GN=Smad4 PE=1 SV=1) HSP 1 Score: 307.76 bits (787), Expect = 7.022e-95 Identity = 156/233 (66.95%), Postives = 185/233 (79.40%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 320 PEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQP 550 HSP 2 Score: 182.57 bits (462), Expect = 1.978e-48 Identity = 94/140 (67.14%), Postives = 120/140 (85.71%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTS 493
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D+S L+L S
Sbjct: 11 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQS 150
BLAST of smad4 vs. UniProt/SwissProt
Match: sp|P97471|SMAD4_MOUSE (Mothers against decapentaplegic homolog 4 OS=Mus musculus OX=10090 GN=Smad4 PE=1 SV=2) HSP 1 Score: 307.375 bits (786), Expect = 7.514e-95 Identity = 156/233 (66.95%), Postives = 185/233 (79.40%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 319 PEYWCSIAYFEMDVQVGETFKVPSSCPVVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQP 549 HSP 2 Score: 182.57 bits (462), Expect = 1.779e-48 Identity = 94/145 (64.83%), Postives = 122/145 (84.14%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSSIEDL 508
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D+S L+L S+ +
Sbjct: 11 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSM 155
BLAST of smad4 vs. UniProt/SwissProt
Match: sp|Q9GKQ9|SMAD4_PIG (Mothers against decapentaplegic homolog 4 OS=Sus scrofa OX=9823 GN=SMAD4 PE=2 SV=1) HSP 1 Score: 307.375 bits (786), Expect = 8.141e-95 Identity = 156/233 (66.95%), Postives = 185/233 (79.40%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 320 PEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQP 550 HSP 2 Score: 182.57 bits (462), Expect = 2.017e-48 Identity = 94/140 (67.14%), Postives = 120/140 (85.71%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTS 493
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D+S L+L S
Sbjct: 11 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQS 150
BLAST of smad4 vs. UniProt/SwissProt
Match: sp|Q13485|SMAD4_HUMAN (Mothers against decapentaplegic homolog 4 OS=Homo sapiens OX=9606 GN=SMAD4 PE=1 SV=1) HSP 1 Score: 307.375 bits (786), Expect = 9.744e-95 Identity = 156/233 (66.95%), Postives = 185/233 (79.40%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 320 PEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQP 550 HSP 2 Score: 183.341 bits (464), Expect = 9.731e-49 Identity = 104/191 (54.45%), Postives = 139/191 (72.77%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSSIEDLDDTESPSVMDSESVNETLKYISNSSNGPDDSLHMRARMTLQYGSSN 646
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D+S L+L S+ +PS M + +Y+ + P S + T+Q+ SN
Sbjct: 11 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSN--------APSSMMVKD-----EYVHDFEGQPSLSTEGHSIQTIQHPPSN 188
BLAST of smad4 vs. TrEMBL
Match: F1DT95 (Smad4 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 614.764 bits (1584), Expect = 0.000e+0 Identity = 291/291 (100.00%), Postives = 291/291 (100.00%), Query Frame = 2
Query: 989 FNTSSSSWNQNQGFDRPPTTSAQPLQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKPTRHFFANYYNQSQSGLGPSGPLHPN 1861
FNTSSSSWNQNQGFDRPPTTSAQPLQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKPTRHFFANYYNQSQSGLGPSGPLHPN
Sbjct: 49 FNTSSSSWNQNQGFDRPPTTSAQPLQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKPTRHFFANYYNQSQSGLGPSGPLHPN 339
BLAST of smad4 vs. TrEMBL
Match: H2KTB1 (Mothers against decapentaplegic homolog 4 OS=Clonorchis sinensis OX=79923 GN=CLF_113096 PE=4 SV=1) HSP 1 Score: 395.201 bits (1014), Expect = 3.670e-129 Identity = 200/304 (65.79%), Postives = 223/304 (73.36%), Query Frame = 2
Query: 1055 QPLQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSV---------------------ANQGS------------------NSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKPTRHFFANYYNQSQSGLGPSGP 1849
QP+ LT QRPPE+WC IAYFEL+QQVGELFKVPSQYS VTVDGYTDPSSPNRFCLGQLSNVHRSE SEKSRLYIGKGVEL+NVGEGDVWIRCLS SVFVQSYYLDREAGR+PGDAVHKIYPGAYIKVFDIRQCH+QMK + AQLA QAAVV GS S T++ +NQ + L+ L+ A +GVDDL C+LRLSFVKGWGPDYPR +IKETPCWIEI+L+RPLQLLDEVLQ M +N+ KPTRHFF Y++Q + P P
Sbjct: 30 QPVPVLTTQRPPEFWCNIAYFELDQQVGELFKVPSQYSRVTVDGYTDPSSPNRFCLGQLSNVHRSEQSEKSRLYIGKGVELDNVGEGDVWIRCLSEFSVFVQSYYLDREAGRRPGDAVHKIYPGAYIKVFDIRQCHEQMKLLAHSAQLAAEHQAAVVVGSIPSPTANAPPISPLMSQLPPGPSQTNAIQSNQNAMGGGGGNSGGGAGAGSGAGGLSPLATADVGVDDLRRLCMLRLSFVKGWGPDYPRRSIKETPCWIEIQLHRPLQLLDEVLQGMPLNDRKPTRHFFP-YFSQPTNCPAPRLP 332
BLAST of smad4 vs. TrEMBL
Match: A0A5J4NB80 (Mothers against decapentaplegic 4 OS=Paragonimus westermani OX=34504 GN=DEA37_0010167 PE=4 SV=1) HSP 1 Score: 392.889 bits (1008), Expect = 5.641e-129 Identity = 196/271 (72.32%), Postives = 214/271 (78.97%), Query Frame = 2
Query: 1070 LTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSIT-------------------SSVANQGSNSL--NNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKPTRHFFANYYNQ 1819
LT QRPPE+WC IAYFEL+QQVGELFKVPSQYS VTVDGYTDPSSPNRFCLGQLSNVHRSE SEKSRLYIGKGVEL+NVGEGDVWIRCLS SVFVQSYYLDREAGR+PGDAVHKIYPGAYIKVFDIRQCH+QMK + AQLA QAAVV GS S T S+ N+L + L A +GVDDL C+LRLSFVKGWGPDYPR +IKETPCWIEI+L+RPLQLLDEVLQ M +N+ KPTRHFF Y++Q
Sbjct: 2 LTTQRPPEFWCNIAYFELDQQVGELFKVPSQYSRVTVDGYTDPSSPNRFCLGQLSNVHRSEQSEKSRLYIGKGVELDNVGEGDVWIRCLSEFSVFVQSYYLDREAGRRPGDAVHKIYPGAYIKVFDIRQCHEQMKLLAHSAQLAAEHQAAVVVGSLPSPTVCGGPPLASHLLPTPGPSPSNTVPPSPNTLGMSPLVTADVGVDDLRRLCMLRLSFVKGWGPDYPRRSIKETPCWIEIQLHRPLQLLDEVLQGMPLNDRKPTRHFFP-YFSQ 271
BLAST of smad4 vs. TrEMBL
Match: C1LFV7 (MAD homolog 4 OS=Schistosoma japonicum OX=6182 GN=Smad4 PE=2 SV=1) HSP 1 Score: 393.66 bits (1010), Expect = 5.539e-127 Identity = 192/274 (70.07%), Postives = 213/274 (77.74%), Query Frame = 2
Query: 1055 QPLQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKAS------------------TEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKPTRHFFANYYNQS 1822
QPL LT QRPPEYWC IAYFEL+QQVGELFKVPSQYS VTVDGYTDPSSPNRFCLGQLSNVHRSE SEKSRLYIGKGVEL+NVGEGDVWIRCLS SVFVQSYYLDREAGR PGDAVHKIYPGAYIKVFDIRQCH++MK + T L A+ G S S + S+ S + ++ AG+GVDDL C+LRLSFVKGWGPDYPR +IKETPCWIEI+L+RPLQLLDEVLQ+M +N+ KPTRHFF+ Y+ QS
Sbjct: 161 QPLPVLTTQRPPEYWCNIAYFELDQQVGELFKVPSQYSRVTVDGYTDPSSPNRFCLGQLSNVHRSEQSEKSRLYIGKGVELDNVGEGDVWIRCLSEFSVFVQSYYLDREAGRAPGDAVHKIYPGAYIKVFDIRQCHEEMKSLAQSSHAAAVRQAAAVVGSPTTNDLLSQLPGALPLGPSQSASGSLPGTLSTGASIMATAGVGVDDLRRLCMLRLSFVKGWGPDYPRRSIKETPCWIEIQLHRPLQLLDEVLQAMPLNDLKPTRHFFS-YFPQS 433
BLAST of smad4 vs. TrEMBL
Match: A0A4Z2CSQ4 (Mothers against decapentaplegic member 4 isoform 2 OS=Schistosoma japonicum OX=6182 GN=EWB00_007850 PE=4 SV=1) HSP 1 Score: 395.201 bits (1014), Expect = 1.459e-126 Identity = 192/274 (70.07%), Postives = 213/274 (77.74%), Query Frame = 2
Query: 1055 QPLQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKAS------------------TEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKPTRHFFANYYNQS 1822
QPL LT QRPPEYWC IAYFEL+QQVGELFKVPSQYS VTVDGYTDPSSPNRFCLGQLSNVHRSE SEKSRLYIGKGVEL+NVGEGDVWIRCLS SVFVQSYYLDREAGR PGDAVHKIYPGAYIKVFDIRQCH++MK + T L A+ G S S + S+ S + ++ AG+GVDDL C+LRLSFVKGWGPDYPR +IKETPCWIEI+L+RPLQLLDEVLQ+M +N+ KPTRHFF+ Y+ QS
Sbjct: 230 QPLPVLTTQRPPEYWCNIAYFELDQQVGELFKVPSQYSRVTVDGYTDPSSPNRFCLGQLSNVHRSEQSEKSRLYIGKGVELDNVGEGDVWIRCLSEFSVFVQSYYLDREAGRAPGDAVHKIYPGAYIKVFDIRQCHEEMKSLAQSSHAAAVRQAAAVVGSPTTNDLLSQLPGALPLGPSQSASGSLPGTLSTGASIMATAGVGVDDLRRLCMLRLSFVKGWGPDYPRRSIKETPCWIEIQLHRPLQLLDEVLQAMPLNDLKPTRHFFS-YFPQS 502
BLAST of smad4 vs. Ensembl Cavefish
Match: smad4a (mothers against decapentaplegic homolog 4 [Source:NCBI gene;Acc:103029394]) HSP 1 Score: 305.834 bits (782), Expect = 9.138e-96 Identity = 156/233 (66.95%), Postives = 185/233 (79.40%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 270 PEYWCSIAYFEMDVQVGETFKVPSNCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQP 500 HSP 2 Score: 184.882 bits (468), Expect = 1.415e-50 Identity = 101/178 (56.74%), Postives = 140/178 (78.65%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSS---IEDLDDTESPSVM-DSESVNETLKYISNSSNGPD 595
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C + +++K D VC+NPYHY+RVV + +D+S L+L+ S ++D D + PS + SE +T+++ + P+
Sbjct: 8 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQFAFDLKCDSVCVNPYHYERVVSAGIDLSGLTLSGSGSLVKDEYDYDCPSSLPSSEGHIQTIQHPPSRPVAPE 185
BLAST of smad4 vs. Ensembl Cavefish
Match: smad4a (mothers against decapentaplegic homolog 4 [Source:NCBI gene;Acc:103029394]) HSP 1 Score: 306.605 bits (784), Expect = 1.155e-95 Identity = 156/233 (66.95%), Postives = 185/233 (79.40%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 302 PEYWCSIAYFEMDVQVGETFKVPSNCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQP 532 HSP 2 Score: 185.267 bits (469), Expect = 1.703e-50 Identity = 101/178 (56.74%), Postives = 140/178 (78.65%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSS---IEDLDDTESPSVM-DSESVNETLKYISNSSNGPD 595
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C + +++K D VC+NPYHY+RVV + +D+S L+L+ S ++D D + PS + SE +T+++ + P+
Sbjct: 12 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQFAFDLKCDSVCVNPYHYERVVSAGIDLSGLTLSGSGSLVKDEYDYDCPSSLPSSEGHIQTIQHPPSRPVAPE 189
BLAST of smad4 vs. Ensembl Cavefish
Match: smad4a (mothers against decapentaplegic homolog 4 [Source:NCBI gene;Acc:103029394]) HSP 1 Score: 306.22 bits (783), Expect = 1.586e-95 Identity = 156/233 (66.95%), Postives = 185/233 (79.40%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 302 PEYWCSIAYFEMDVQVGETFKVPSNCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQP 532 HSP 2 Score: 174.481 bits (441), Expect = 1.285e-46 Identity = 97/175 (55.43%), Postives = 133/175 (76.00%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSSIEDLDDTESPSVM-DSESVNETLKYISNSSNGPD 595
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C + +++K D VC+NPYHY+RVV + + S ++D D + PS + SE +T+++ + P+
Sbjct: 8 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQFAFDLKCDSVCVNPYHYERVVSAGIG-------SLVKDEYDYDCPSSLPSSEGHIQTIQHPPSRPVAPE 175
BLAST of smad4 vs. Ensembl Cavefish
Match: smad4a (mothers against decapentaplegic homolog 4 [Source:NCBI gene;Acc:103029394]) HSP 1 Score: 306.605 bits (784), Expect = 1.622e-95 Identity = 156/233 (66.95%), Postives = 185/233 (79.40%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 312 PEYWCSIAYFEMDVQVGETFKVPSNCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQP 542 HSP 2 Score: 185.652 bits (470), Expect = 1.756e-50 Identity = 101/178 (56.74%), Postives = 140/178 (78.65%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSS---IEDLDDTESPSVM-DSESVNETLKYISNSSNGPD 595
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C + +++K D VC+NPYHY+RVV + +D+S L+L+ S ++D D + PS + SE +T+++ + P+
Sbjct: 8 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQFAFDLKCDSVCVNPYHYERVVSAGIDLSGLTLSGSGSLVKDEYDYDCPSSLPSSEGHIQTIQHPPSRPVAPE 185
BLAST of smad4 vs. Ensembl Cavefish
Match: smad4b (SMAD family member 4b [Source:ZFIN;Acc:ZDB-GENE-060130-140]) HSP 1 Score: 305.064 bits (780), Expect = 1.684e-95 Identity = 154/233 (66.09%), Postives = 184/233 (78.97%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
P+YWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R QLLDEVL +M + + +P
Sbjct: 261 PDYWCSIAYFEMDVQVGETFKVPSSCPVVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRAFQLLDEVLHTMPIADPQP 491 HSP 2 Score: 172.17 bits (435), Expect = 3.845e-46 Identity = 89/126 (70.63%), Postives = 112/126 (88.89%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRV 451
SNDAC+SIV+SL+CHRQGGES++FS++AIESLVKKLKEK++ELD LITAVTT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C + +++K D VC+NPYHY+RV
Sbjct: 9 TSNDACLSIVHSLMCHRQGGESESFSKRAIESLVKKLKEKKDELDSLITAVTTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQFAFDLKCDSVCVNPYHYERV 134
BLAST of smad4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010406.1 (pep scaffold:Pmarinus_7.0:GL476769:63937:79511:-1 gene:ENSPMAG00000009414.1 transcript:ENSPMAT00000010406.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 314.309 bits (804), Expect = 6.874e-99 Identity = 158/233 (67.81%), Postives = 188/233 (80.69%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PE+WC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+++SEK+RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYPGAYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPRH+IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 335 PEFWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTDASEKARLHIGKGVQLECRGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPGAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRHSIKETPCWIEIHLHRALQLLDEVLHTMPMADPRP 565 HSP 2 Score: 192.586 bits (488), Expect = 1.691e-53 Identity = 110/197 (55.84%), Postives = 145/197 (73.60%), Query Frame = 2
Query: 38 KMLMSLNDPSPVNSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSS-----------IEDLDDTESPS-VMDSESVNETLKYISNSSNGP 592
+ MSL PS SNDAC+SIV+SLLCHRQGGES+ F+++AIESLV+KLKEK++ELD LITAVTT+G P+ CVTI +TLDGRLQ+AGRK FPHV+Y+RIWRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D++ L+L S+ I D +SPS + SE + +L+ IS+ + P
Sbjct: 1 RKTMSLVTPS---SNDACLSIVHSLLCHRQGGESETFAKRAIESLVRKLKEKKDELDSLITAVTTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVVYARIWRWPDLHKNELKHVKFCQYAFDLKADSVCVNPYHYERVVSPCIDLAGLTLQSTGPSSLLVKDEPILDCLAADSPSGRLASEGAHGSLQLISHQAQAP 194
BLAST of smad4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010514.1 (pep scaffold:Pmarinus_7.0:GL482855:12566:14836:-1 gene:ENSPMAG00000009525.1 transcript:ENSPMAT00000010514.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 211.846 bits (538), Expect = 4.442e-65 Identity = 114/176 (64.77%), Postives = 136/176 (77.27%), Query Frame = 2
Query: 1259 EKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
+ RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYPGAYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LRLSFVKGWGPDYPR +IK+TPCWIEI L+R LQLLD+VL +M + + P
Sbjct: 1 DAGRLHIGKGVQLECRGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPGAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRLSFVKGWGPDYPRQSIKQTPCWIEIHLHRALQLLDQVLHTMPMADPTP 174
BLAST of smad4 vs. Ensembl Sea Lamprey
Match: smad4a (SMAD family member 4a [Source:ZFIN;Acc:ZDB-GENE-990603-8]) HSP 1 Score: 188.734 bits (478), Expect = 8.707e-55 Identity = 99/150 (66.00%), Postives = 126/150 (84.00%), Query Frame = 2
Query: 47 MSLNDPSPVNSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSS 496
MSL P+ SNDAC+SIV+SL+CHRQGGES++FS++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+RIWRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D+ +L+L S
Sbjct: 4 MSLGTPT---SNDACLSIVHSLMCHRQGGESESFSKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARIWRWPDLHKNELKHLKFCQYAFDLKADSVCVNPYHYERVVSPGLDLGQLTLHGS 150
BLAST of smad4 vs. Ensembl Sea Lamprey
Match: SMAD3 (SMAD family member 3 [Source:HGNC Symbol;Acc:HGNC:6769]) HSP 1 Score: 161.384 bits (407), Expect = 2.648e-45 Identity = 97/226 (42.92%), Postives = 131/226 (57.96%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSM 1765
P +WC+I Y+ELNQ+VGE F SQ S +TVDG+TDPS+ RFCLG LSNV+R++ E +R +IG+GV L +G G+V+ CLS ++FVQS ++ G P V KI PG +K+F+ +Q+ AA++A S +QG + V L+ C +R+SFVKGWG +Y R I TPCWIE+ LN PLQ LD VL M
Sbjct: 67 PAFWCSIGYYELNQRVGETFHA-SQPS-LTVDGFTDPSNSERFCLGLLSNVNRNQVVEMTRRHIGRGVRLYYIG-GEVFAECLSESAIFVQSPNCNQRYGWHPA-TVCKIPPGCNLKIFN----NQEF--------------AALLAQS--------VHQGFEA----------VYQLTRMCTIRMSFVKGWGAEYRRQTITSTPCWIELHLNGPLQWLDRVLTQM 252
BLAST of smad4 vs. Ensembl Sea Lamprey
Match: SMAD3 (SMAD family member 3 [Source:HGNC Symbol;Acc:HGNC:6769]) HSP 1 Score: 162.925 bits (411), Expect = 4.669e-44 Identity = 99/241 (41.08%), Postives = 141/241 (58.51%), Query Frame = 2
Query: 1043 TTSAQPLQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSM 1765
+ S+ LQ +T+ P +WC+I+Y+ELNQ+VGE F SQ S +TVDG+TDPS+ RFCLG LSN++R+++ E +R +IG+GV L +G G+V+ CLS ++FVQS ++ G P V KI PG +K+F+ ++ AA++A S NQG + V L+ C +R+SFVKGWG +Y R + TPCWIE+ LN PLQ LD+VL M
Sbjct: 224 SVSSADLQPVTYCEP-AFWCSISYYELNQRVGETFHA-SQPS-LTVDGFTDPSNSERFCLGLLSNINRNQTVEMTRRHIGRGVRLYYIG-GEVFAECLSESAIFVQSPNCNQRYGWHPA-TVCKIPPGCNLKIFNNQEF------------------AALLAQS--------VNQGFEA----------VYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQM 423 HSP 2 Score: 96.6709 bits (239), Expect = 1.399e-21 Identity = 43/95 (45.26%), Postives = 60/95 (63.16%), Query Frame = 2
Query: 248 TGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHK-NELRQNKCCIYGYEMKLDYVCINPYHYDR-----VVPSAVDISELSLTSSIEDLDD 514
T C+TIP++LDGRLQ++ RK PHVIY R+WRWP+LH +EL C + + +K D VC+NPYHY R V+P + + + + LDD
Sbjct: 62 TKCITIPRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELHAIDACEFAFHLKKDEVCVNPYHYQRVETPAVLPPVLVPRHTDIPAELPKLDD 156
BLAST of smad4 vs. Ensembl Nematostella
Match: EDO31382 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SY32]) HSP 1 Score: 292.352 bits (747), Expect = 2.352e-92 Identity = 143/233 (61.37%), Postives = 164/233 (70.39%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PE WC+IAYFEL+QQVGE+FKV S VTVDGY DPS NRFCLGQLSNVHR+E+SE++RL+IGKGV+L+ GEGDVW+RCLS HSVFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+R QM + S +GVDDL C+LRLSFVKGWGPDYPR +IKETPCWIEI L+R LQLLDE+L +M +NE +P
Sbjct: 206 PENWCSIAYFELDQQVGEIFKVTSNCPSVTVDGYVDPSGGNRFCLGQLSNVHRTEASERARLHIGKGVQLDVRGEGDVWVRCLSEHSVFVQSYYLDREAGRCPGDAVHKIYPSAYIKVFDLRALLPQMGQTS----------------------------------------VGVDDLRRLCILRLSFVKGWGPDYPRKSIKETPCWIEIHLHRALQLLDEILITMPINEPRP 398 HSP 2 Score: 175.252 bits (443), Expect = 1.591e-48 Identity = 94/143 (65.73%), Postives = 123/143 (86.01%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSSIE 502
S+DAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+T++GT P+ CVTI +TLDGRLQ+AGRK FPHVIY+RIWRWP+LHKNELR K C + +++K D VC+NP+HY+RVV + DI+ LSL+ + E
Sbjct: 6 TSSDACLSIVHSLMCHRQGGESEAFAKRAIESLVKKLKEKKDELDSLITAITSAGTHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARIWRWPDLHKNELRHVKYCQFAFDLKCDSVCVNPFHYERVV--SPDIAGLSLSRNSE 146
BLAST of smad4 vs. Ensembl Nematostella
Match: EDO39594 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9L0]) HSP 1 Score: 159.073 bits (401), Expect = 1.380e-42 Identity = 97/238 (40.76%), Postives = 132/238 (55.46%), Query Frame = 2
Query: 1052 AQPLQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSM 1765
AQP+Q P YWC+I+Y+E+N +VGE F SQ S +TVDG+TDPSS +RFCLG LSN++R+ E +R +IGKGV L +G G+V+ CLS S+FVQS ++ P V KI PG +K+F+ ++ Q + S NQG ++ L+ C +R+SFVKGWG +Y R + TPCWIE+ LN PLQ LD+VL M
Sbjct: 219 AQPIQYTE----PTYWCSISYYEMNTRVGETFHA-SQPS-LTVDGFTDPSSSDRFCLGLLSNINRNPPIEMTRKHIGKGVRLYYIG-GEVFAECLSDSSIFVQSPNCNQRYNWHPA-TVCKIPPGCNLKIFNNQEFAQ--------------------------LLSQSVNQGFEAVYALTR----------MCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQM 412 HSP 2 Score: 93.9745 bits (232), Expect = 2.127e-20 Identity = 41/78 (52.56%), Postives = 56/78 (71.79%), Query Frame = 2
Query: 221 AVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHK-NELRQNKCCIYGYEMKLDYVCINPYHYDRV 451
A+T G T CVTIP++LDGRLQ++ RK PHVIY R+WRWP+L +ELR + C + + +K + VC+NP+HY RV
Sbjct: 52 AITNPGVA-TKCVTIPRSLDGRLQVSHRKGLPHVIYCRLWRWPDLQSHHELRPIEACEFAFSLKKEEVCVNPFHYQRV 128
BLAST of smad4 vs. Ensembl Nematostella
Match: EDO47037 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNC1]) HSP 1 Score: 153.295 bits (386), Expect = 2.445e-40 Identity = 97/252 (38.49%), Postives = 138/252 (54.76%), Query Frame = 2
Query: 1031 DRPPTTSAQPLQALTH-----QRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPN--RFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSM 1765
+ PP+ S Q +A++H + P WC+IAY+ELN +VGELF S + + VDG+TDP++ N RFCLG LSNV+R+ + E +R +IGKGV L VG G+V+ CLS ++FVQS + G P V KI G +K+F+ ++ Q + S N G + V +L+ C +R+SFVKGWG +Y R ++ TPCWIE+ L+ PLQ LD+VL M
Sbjct: 214 NSPPSIS-QSAEAMSHVTAVNYQEPLSWCSIAYYELNNRVGELFHAKS--TSLIVDGFTDPNTTNSERFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVG-GEVYAECLSDSAIFVQSRNCNHSHGFHP-TTVCKIPSGCTLKIFNNQEFAQ--------------------------LLSQSVNYGFEA----------VYELTKMCSIRISFVKGWGAEYHRQDVTSTPCWIEVHLHGPLQWLDKVLSQM 424 HSP 2 Score: 99.7525 bits (247), Expect = 2.695e-22 Identity = 61/118 (51.69%), Postives = 82/118 (69.49%), Query Frame = 2
Query: 101 VNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHK-NELRQNKCCIYGYEMKLDYVCINPYHYDRV 451
V LL +QG E + ++ KAIESLVKKLK+K+ L+ L A++ Q + CVTI ++LDGRLQ++ RK PHVIY R+WRWP+L +EL+ +CC Y + +K VCINPYHY RV
Sbjct: 12 VKRLLGWKQGDEEEKWAEKAIESLVKKLKKKKGALEDLEKALSNPNQQ-SKCVTIARSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEYAFGLKQKEVCINPYHYHRV 128
BLAST of smad4 vs. Ensembl Nematostella
Match: EDO37590 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFA8]) HSP 1 Score: 130.568 bits (327), Expect = 5.939e-36 Identity = 63/110 (57.27%), Postives = 91/110 (82.73%), Query Frame = 2
Query: 122 RQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRV 451
RQGGE++ F+++AIESLVKKL++K +EL+ LI+ +TT+G QP+ CVTI +TLDGRLQ+ RK FPHVIY+R+WRWP++ K E++ C +GY++K + VC+NPYHY+R+
Sbjct: 1 RQGGETEKFAKRAIESLVKKLRKKTDELESLISTITTNGAQPSKCVTIQRTLDGRLQVCERKGFPHVIYARLWRWPDIQKMEMKHLDFCRFGYDLKYESVCVNPYHYERI 110
BLAST of smad4 vs. Ensembl Nematostella
Match: EDO37591 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFA9]) HSP 1 Score: 125.946 bits (315), Expect = 2.937e-34 Identity = 65/111 (58.56%), Postives = 88/111 (79.28%), Query Frame = 2
Query: 125 QGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVP 457
Q E+ +FSR+AIESLVKKLK+K ELD LI A+T+ G + CVT+P+T+DGRLQ+ +K FPHVIY+R+WRWP++HK E+R + C +GY++K + VC+NPYHYDRV P
Sbjct: 1 QSVEADSFSRRAIESLVKKLKKKFYELDSLIIAITSKGRTASKCVTVPRTMDGRLQVGEKKDFPHVIYARLWRWPDVHKMEMRHKEYCQFGYDLKNEKVCVNPYHYDRVQP 111
BLAST of smad4 vs. Ensembl Medaka
Match: smad4b (mothers against decapentaplegic homolog 4 [Source:NCBI gene;Acc:101163504]) HSP 1 Score: 305.834 bits (782), Expect = 2.510e-96 Identity = 156/233 (66.95%), Postives = 185/233 (79.40%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 218 PEYWCSIAYFEMDVQVGETFKVPSTCPVVTVDGYVDPSGGDRFCLGQLSNVHRTENIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQP 448 HSP 2 Score: 181.03 bits (458), Expect = 1.255e-49 Identity = 92/141 (65.25%), Postives = 123/141 (87.23%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSS 496
SNDAC+SIV+SL+CHRQGGES++F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHV+Y+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D+S L++++S
Sbjct: 13 TSNDACLSIVHSLMCHRQGGESESFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVVYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSTLTISNS 153
BLAST of smad4 vs. Ensembl Medaka
Match: SMAD4 (mothers against decapentaplegic homolog 4 [Source:NCBI gene;Acc:101168872]) HSP 1 Score: 304.679 bits (779), Expect = 3.854e-95 Identity = 155/233 (66.52%), Postives = 185/233 (79.40%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKP 1786
P+YWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEVL +M + + +P
Sbjct: 278 PDYWCSIAYFEMDVQVGETFKVPSTCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQP 508 HSP 2 Score: 187.578 bits (475), Expect = 1.877e-51 Identity = 102/180 (56.67%), Postives = 137/180 (76.11%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSS-------IEDLDDTESPSVMDSESVNETLKYISNSSNGP 592
SNDAC+SIV+SL+CHRQGGES+ F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHVIY+R+WRWP+LHKNEL+ K C + +++K D VC+NPYHY+RVV +D+S L+LTSS ++D D + P + S +L+ I + + P
Sbjct: 8 TSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQFAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLTSSGPSSALIVKDEYDFDGPQSIPSVEGGHSLQTIQHPPSNP 187
BLAST of smad4 vs. Ensembl Medaka
Match: smad4b (mothers against decapentaplegic homolog 4 [Source:NCBI gene;Acc:101163504]) HSP 1 Score: 296.975 bits (759), Expect = 2.289e-92 Identity = 153/222 (68.92%), Postives = 178/222 (80.18%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEV 1753
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEV
Sbjct: 271 PEYWCSIAYFEMDVQVGETFKVPSTCPVVTVDGYVDPSGGDRFCLGQLSNVHRTENIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEV 490 HSP 2 Score: 180.644 bits (457), Expect = 4.958e-49 Identity = 92/141 (65.25%), Postives = 123/141 (87.23%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSS 496
SNDAC+SIV+SL+CHRQGGES++F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHV+Y+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D+S L++++S
Sbjct: 8 TSNDACLSIVHSLMCHRQGGESESFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVVYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSTLTISNS 148
BLAST of smad4 vs. Ensembl Medaka
Match: smad4b (mothers against decapentaplegic homolog 4 [Source:NCBI gene;Acc:101163504]) HSP 1 Score: 296.59 bits (758), Expect = 2.866e-92 Identity = 153/222 (68.92%), Postives = 178/222 (80.18%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEV 1753
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEV
Sbjct: 261 PEYWCSIAYFEMDVQVGETFKVPSTCPVVTVDGYVDPSGGDRFCLGQLSNVHRTENIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEV 480 HSP 2 Score: 171.014 bits (432), Expect = 8.816e-46 Identity = 88/131 (67.18%), Postives = 114/131 (87.02%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAV 466
SNDAC+SIV+SL+CHRQGGES++F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHV+Y+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +
Sbjct: 8 TSNDACLSIVHSLMCHRQGGESESFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVVYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGI 138
BLAST of smad4 vs. Ensembl Medaka
Match: smad4b (mothers against decapentaplegic homolog 4 [Source:NCBI gene;Acc:101163504]) HSP 1 Score: 296.59 bits (758), Expect = 5.883e-92 Identity = 153/222 (68.92%), Postives = 178/222 (80.18%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEV 1753
PEYWC+IAYFE++ QVGE FKVPS VTVDGY DPS +RFCLGQLSNVHR+E+ E++RL+IGKGV+L GEGDVW+RCLS H+VFVQSYYLDREAGR PGDAVHKIYP AYIKVFD+RQCH+QM++ + AQ A QAA VAG+ S + SL+ +AAGIGVDDL C+LR+SFVKGWGPDYPR +IKETPCWIEI L+R LQLLDEV
Sbjct: 271 PEYWCSIAYFEMDVQVGETFKVPSTCPVVTVDGYVDPSGGDRFCLGQLSNVHRTENIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLS--AAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEV 490 HSP 2 Score: 181.03 bits (458), Expect = 6.004e-49 Identity = 92/141 (65.25%), Postives = 123/141 (87.23%), Query Frame = 2
Query: 74 NSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSS 496
SNDAC+SIV+SL+CHRQGGES++F+++AIESLVKKLKEK++ELD LITA+TT+G P+ CVTI +TLDGRLQ+AGRK FPHV+Y+R+WRWP+LHKNEL+ K C Y +++K D VC+NPYHY+RVV +D+S L++++S
Sbjct: 8 TSNDACLSIVHSLMCHRQGGESESFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVVYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSTLTISNS 148
BLAST of smad4 vs. Planmine SMEST
Match: SMESG000013170.1 (SMESG000013170.1) HSP 1 Score: 1087.79 bits (2812), Expect = 0.000e+0 Identity = 604/605 (99.83%), Postives = 604/605 (99.83%), Query Frame = 2
Query: 47 MSLNDPSPVNSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSSIEDLDDTESPSVMDSESVNETLKYISNSSNGPDDSLHMRARMTLQYGSSNAEYSLDDPDPLKSRIGXXXXXXXXXXXXXXXXXXFKPRIGQDGDNIPTTIKYENSVVAHYCQNSPSNGNFKIASLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFNTSSSSWNQNQGFDRPPTTSAQPLQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKPTRHFFANYYNQSQSGLGPSGPLHPN 1861
MSLNDPSPVNSNDACMSIVNSLLCHRQGGESQNFSRKAIESLVKKLKEKREELDFLITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSSIEDLDDTESPSVMDSESVNETLKYISNSSNGPDDSLHMRARMTLQYGSSNAEYSLDDPDPLKSRIGQSLDVQSMSDSSGSPSDQFKPRIGQDGDNIPTTIKYENSVVAHYCQNSPSNGNFKIASLTGRGGSQNSQGSSASQQQNQTQRNTNQPTQSTPQQSRPNFN SSSSWNQNQGFDRPPTTSAQPLQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKPTRHFFANYYNQSQSGLGPSGPLHPN
Sbjct: 1 MSLNDPSPVNSNDACMSIVNSLLCHRQGGESQNFSRKAIESLVKKLKEKREELDFLITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDISELSLTSSIEDLDDTESPSVMDSESVNETLKYISNSSNGPDDSLHMRARMTLQYGSSNAEYSLDDPDPLKSRIGQSLDVQSMSDSSGSPSDQFKPRIGQDGDNIPTTIKYENSVVAHYCQNSPSNGNFKIASLTGRGGSQNSQGSSASQQQNQTQRNTNQPTQSTPQQSRPNFNNSSSSWNQNQGFDRPPTTSAQPLQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSMSVNEYKPTRHFFANYYNQSQSGLGPSGPLHPN 605
BLAST of smad4 vs. Planmine SMEST
Match: SMESG000029614.1 (SMESG000029614.1) HSP 1 Score: 209.534 bits (532), Expect = 5.114e-57 Identity = 92/146 (63.01%), Postives = 117/146 (80.14%), Query Frame = 2
Query: 1061 LQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQL 1498
L++L + W TI+YFE++ QVGE FKVP + V++DGYTDPSSPNRF LG+L N+HR +S EK+RLYIG+G++L +VG+G++WIRCLS SVFVQSYYLD +A RQPG AVHKIYPGAYIKVFD+RQC++Q+K A E L
Sbjct: 629 LESLLRNTSTDIWTTISYFEMDTQVGECFKVPVNFLKVSIDGYTDPSSPNRFSLGKLVNIHRVDSVEKARLYIGRGIDLESVGDGEIWIRCLSGFSVFVQSYYLDHKAYRQPGGAVHKIYPGAYIKVFDVRQCYEQIKAALIEHHL 774 HSP 2 Score: 174.096 bits (440), Expect = 4.157e-45 Identity = 87/134 (64.93%), Postives = 115/134 (85.82%), Query Frame = 2
Query: 71 VNSNDACMSIVNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHKNELRQNKCCIYGYEMKLDYVCINPYHYDRVVPSAVDI 472
VNS+DAC+SIV SLL R+ GES+ F+++AIESLVKKLK+KR+ELD LI AV+T+G PT CVTI +TLDGRLQ+AGRKCFPHVIY+RIWRWP+LHKNEL+ K C+YG+++K + VC+NPYHY+R++ + ++
Sbjct: 12 VNSSDACLSIVQSLLTRRKSGESEGFAKRAIESLVKKLKDKRDELDALIVAVSTAGNHPTKCVTIQRTLDGRLQVAGRKCFPHVIYARIWRWPDLHKNELKHCKFCVYGFDLKQESVCVNPYHYERIITAPFNV 145 HSP 3 Score: 96.2857 bits (238), Expect = 3.414e-20 Identity = 39/56 (69.64%), Postives = 47/56 (83.93%), Query Frame = 2
Query: 1595 AAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQS 1762
G+ VDDL C+LRLSFVKGWGPDYPRH+IKETPCWIEI L+R L++LD +LQ+
Sbjct: 894 TTGLDVDDLWELCILRLSFVKGWGPDYPRHSIKETPCWIEIHLHRALKILDSMLQA 949
BLAST of smad4 vs. Planmine SMEST
Match: SMESG000011457.1 (SMESG000011457.1) HSP 1 Score: 156.377 bits (394), Expect = 5.750e-41 Identity = 104/269 (38.66%), Postives = 141/269 (52.42%), Query Frame = 2
Query: 995 TSSSSWNQNQGFDRPPTTSAQP-----------LQALTHQRPPEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSS-PNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSM 1765
T S + N N D P T +QP +Q++T+Q P + WC+I Y+ELN +VGE F S+ S + VDG+TDPS+ +RFCLG LSNV+R+ + E +R +IGKGV L VG GDV+ CLS S+FVQS + P V KI PG +K+F + + A++ V G V +L+ C +R+SFVKGWG +Y R ++ TPCWIEI LN PL LD+VL M
Sbjct: 217 TPSINGNGNTSSDTPITNISQPQPMDVSSVPKNMQSVTYQEP-KNWCSIVYYELNNRVGEAF-FASKLS-IIVDGFTDPSNNSDRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVG-GDVYAECLSDSSIFVQSRNCNYHHNFHP-TTVCKIPPGCSLKIF-------------SNQEFAFLLSRTVHHGFE-----------------------AVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLNGPLHWLDKVLSQM 444 HSP 2 Score: 96.6709 bits (239), Expect = 5.926e-21 Identity = 61/134 (45.52%), Postives = 89/134 (66.42%), Query Frame = 2
Query: 65 SPVNSNDACMSI----VNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHK-NELRQNKCCIYGYEMKLDYVCINPYHYDRV 451
+PV+S ++ S V LL +QG E + ++ KAI+SLVKKLK+K+ ++ + V ++ + CVTIP++LDGRLQ++ RK PHVIY R+WRWP+L +EL+ ++ C Y + K VCINPYHY RV
Sbjct: 3 TPVSSLNSLFSFTSPAVKRLLGWKQGDEEEKWAEKAIDSLVKKLKKKKGAIES-LEKVLRYPSEQSECVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKASEYCKYPFSAKETEVCINPYHYKRV 135
BLAST of smad4 vs. Planmine SMEST
Match: SMESG000031386.1 (SMESG000031386.1) HSP 1 Score: 155.992 bits (393), Expect = 1.715e-40 Identity = 92/226 (40.71%), Postives = 124/226 (54.87%), Query Frame = 2
Query: 1088 PEYWCTIAYFELNQQVGELFKVPSQYSCVTVDGYTDPSSPNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSM 1765
P+YWC+I Y+EL+ GE F + SC+TVDG+TDP+ +RFCLG +SNV+R+ +E +R +IGKG+ L +G G+V+ CLS ++FVQS + G P V KI P +K+FD Q Q+ LA +++S V L+ C +RLSFVKGWG DY R + TPCWIEI LN PLQ LD VL+ M
Sbjct: 312 PQYWCSIRYYELSVAFGETFHCST--SCLTVDGFTDPAQSDRFCLGLVSNVNRNPQTELARRHIGKGLRLYYIG-GEVFAECLSESAIFVQSPNCNYLNGWHPA-TVCKIPPRCNLKIFD-NQVFAQL--------LA---------------------------DSVSKGFEAVFTLTHMCSIRLSFVKGWGSDYRRQTVTNTPCWIEIHLNGPLQWLDRVLKEM 497 HSP 2 Score: 89.7373 bits (221), Expect = 1.656e-18 Identity = 42/109 (38.53%), Postives = 66/109 (60.55%), Query Frame = 2
Query: 248 TGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLH-KNELRQNKCCIYGYEMKLDYVCINPYHYDRVV-PSAVDISELSLTSSIEDLDDTESPSVMDSESVNETLKY 568
T C+T+ ++LDGRLQ+A +K PH+ Y ++WRWP+LH ++ELR C Y + +K + VCINPYHY R+ P I +++ TE+ + ++N+ L Y
Sbjct: 60 TKCITLQRSLDGRLQVAQKKGLPHIFYCQLWRWPDLHTQHELRPIDTCEYSFHLKKEEVCINPYHYRRIENPVLPPIMVPKNKETVQSSSATETTKTSQNGTLNQLLSY 168
BLAST of smad4 vs. Planmine SMEST
Match: SMESG000056334.1 (SMESG000056334.1) HSP 1 Score: 151.369 bits (381), Expect = 1.143e-39 Identity = 91/237 (38.40%), Postives = 130/237 (54.85%), Query Frame = 2
Query: 1064 QALTHQRPPEYWCTIAYFELNQQVGELF--KVPSQYSCVTVDGYTDPSS-PNRFCLGQLSNVHRSESSEKSRLYIGKGVELNNVGEGDVWIRCLSAHSVFVQSYYLDREAGRQPGDAVHKIYPGAYIKVFDIRQCHQQMKKASTEAQLAWVRQAAVVAGSSNSITSSVANQGSNSLNNLSAAGIGVDDLSPFCVLRLSFVKGWGPDYPRHNIKETPCWIEIKLNRPLQLLDEVLQSM 1765
Q++ +Q P YW T+ Y+ELN +VGE F ++PS + +DG+TDPS+ +RFCLG LSNV+R+ + E +R +IGKGV L VG G+V+ CLS S+FVQS + P V KI PG +K+F R+ +Q+ + VV G + +++ C +R+SFVKGWG +Y R ++ TPCW+EI LN PL+ LD VL M
Sbjct: 206 QSVVYQEP-RYWSTVVYYELNSRVGEAFHARLPS----IIIDGFTDPSNNADRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVG-GEVFAECLSDSSIFVQSRNCNYRHNFHP-STVCKIPPGCSLKIFSNREFAEQLSR-------------TVVHGYE-----------------------AIFEMTRMCTVRMSFVKGWGAEYHRQDVTSTPCWVEIHLNGPLKWLDRVLTKM 399 HSP 2 Score: 91.2781 bits (225), Expect = 2.934e-19 Identity = 57/118 (48.31%), Postives = 80/118 (67.80%), Query Frame = 2
Query: 101 VNSLLCHRQGGESQNFSRKAIXXXXXXXXXXXXXXXXXITAVTTSGTQPTGCVTIPKTLDGRLQIAGRKCFPHVIYSRIWRWPNLHK-NELRQNKCCIYGYEMKLDYVCINPYHYDRV 451
V LL +QG E ++ KA++SLVKKLK+KR L+ L A++ T+ + CVTI ++LDGRLQ++ RK PHVIY R+WRWP+L +EL+ + C Y + K +CINPYHY R+
Sbjct: 20 VKRLLGWKQGDEEDKWAEKAVDSLVKKLKKKRGALESLEKALSNP-TEQSECVTIQRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLEFCKYPFSAKEKEICINPYHYKRI 136 The following BLAST results are available for this feature:
BLAST of smad4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of smad4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of smad4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of smad4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of smad4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of smad4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of smad4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of smad4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of smad4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of smad4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of smad4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of smad4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of smad4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of smad4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Cross References
External references for this transcript
Sequences
The following sequences are available for this feature:
transcript sequence >SMED30011742 ID=SMED30011742|Name=smad4|organism=Schmidtea mediterranea sexual|type=transcript|length=2101bpback to top protein sequence of SMED30011742-orf-1 >SMED30011742-orf-1 ID=SMED30011742-orf-1|Name=SMED30011742-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=608bp MLMSLNDPSPVNSNDACMSIVNSLLCHRQGGESQNFSRKAIESLVKKLKEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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