SMED30011704

Overview
NameSMED30011704
Smed IDSMED30011704
Length (bp)2277
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30011704 (SMED30011704) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30011704 (SMED30011704) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30011704 (SMED30011704) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30011704 (SMED30011704) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30011704

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30011704h1SMcG0011570 SmedASXL_016535SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30011704h1SMcG0011570 SmedASXL_016535SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30011704h1SMcG0011570 dd_Smed_v6_15194_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
zeta neoblastSMED30011704h1SMcG0011570 dd_Smed_v4_15194_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30011704h1SMcG0011570 SMED_36956_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30011704h1SMcG0011570 SMED_08610_V2_1GPL14150PMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30011704h1SMcG0011570 SMED_08610_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Alignments
SMED30011704 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000247:64843..72711 -11131
Homology
BLAST of SMED30011704 vs. Planmine SMEST
Match: SMESG000063079.1 (SMESG000063079.1)

HSP 1 Score: 1402.88 bits (3630), Expect = 0.000e+0
Identity = 719/719 (100.00%), Postives = 719/719 (100.00%), Query Frame = 1
Query:   52 MDESFDISKIVYASQNSKITLKTKQKLDELAAQVAFWNSEVYQMSSNKENRIVNLSKPVISNVKKSFVFSNDKFSANSVKFPLTESRSGNRKSMLDCKGSVREKKAYFESLTQTTPKGKIPIFKVQQSFKNIEYPKIFEVSSRNANESVITEEQNKDDNNISVEDILPPPPYISDESQYGDEEIVVTDKGHRESLKNESTVVSELDASWNNVDTQLNNIXXXXXXXXXXXXXXXXXXXFDEFIFEYGKMTKENDVESLPEKYSKIERIKPRRSLSFNEKVNVIEISDSDSSENLANTPGKNLEFNAKLSYITPDNLQENFESSDEPTICFEMEELKQRSWHLIKDLEAFSSTLSYTPGNLRHVEKCIDIVKNNLLYASVVERIHCQHNSNIGIIADVDIHGVRFSIKDNYVFFNYKSLLARGYDSKISRLPSIYHVALVSRSVAXXXXXXXXXXXXVKRRDADKVGLDYHQSRWLEFFEDIDFLRLSSGFKIIFELYCMNVTRKKHDQSFGTPKRRTPMKKLFYDSPNKFTDSPILLSNVGTFDLTSKDFIVNNNDIIEKNLHLPNSNPFDGCVKLDGSMSFSVASSCEGRLNVYMEVGKIGSWAFRDCQLSHTGLIVGKKSEQTGIIEEVDANIPFFTMKESANLCDFQVCAKANSFSVLLIDQEIKEKMAINRRISTDGKRKYRFLAAAESQDDACRWCKCINDCNDSFKDWSKNID 2208
            MDESFDISKIVYASQNSKITLKTKQKLDELAAQVAFWNSEVYQMSSNKENRIVNLSKPVISNVKKSFVFSNDKFSANSVKFPLTESRSGNRKSMLDCKGSVREKKAYFESLTQTTPKGKIPIFKVQQSFKNIEYPKIFEVSSRNANESVITEEQNKDDNNISVEDILPPPPYISDESQYGDEEIVVTDKGHRESLKNESTVVSELDASWNNVDTQLNNISSDLTRVSSLSSSQSIGSLFDEFIFEYGKMTKENDVESLPEKYSKIERIKPRRSLSFNEKVNVIEISDSDSSENLANTPGKNLEFNAKLSYITPDNLQENFESSDEPTICFEMEELKQRSWHLIKDLEAFSSTLSYTPGNLRHVEKCIDIVKNNLLYASVVERIHCQHNSNIGIIADVDIHGVRFSIKDNYVFFNYKSLLARGYDSKISRLPSIYHVALVSRSVADSTITISDIYSIVKRRDADKVGLDYHQSRWLEFFEDIDFLRLSSGFKIIFELYCMNVTRKKHDQSFGTPKRRTPMKKLFYDSPNKFTDSPILLSNVGTFDLTSKDFIVNNNDIIEKNLHLPNSNPFDGCVKLDGSMSFSVASSCEGRLNVYMEVGKIGSWAFRDCQLSHTGLIVGKKSEQTGIIEEVDANIPFFTMKESANLCDFQVCAKANSFSVLLIDQEIKEKMAINRRISTDGKRKYRFLAAAESQDDACRWCKCINDCNDSFKDWSKNID
Sbjct:    1 MDESFDISKIVYASQNSKITLKTKQKLDELAAQVAFWNSEVYQMSSNKENRIVNLSKPVISNVKKSFVFSNDKFSANSVKFPLTESRSGNRKSMLDCKGSVREKKAYFESLTQTTPKGKIPIFKVQQSFKNIEYPKIFEVSSRNANESVITEEQNKDDNNISVEDILPPPPYISDESQYGDEEIVVTDKGHRESLKNESTVVSELDASWNNVDTQLNNISSDLTRVSSLSSSQSIGSLFDEFIFEYGKMTKENDVESLPEKYSKIERIKPRRSLSFNEKVNVIEISDSDSSENLANTPGKNLEFNAKLSYITPDNLQENFESSDEPTICFEMEELKQRSWHLIKDLEAFSSTLSYTPGNLRHVEKCIDIVKNNLLYASVVERIHCQHNSNIGIIADVDIHGVRFSIKDNYVFFNYKSLLARGYDSKISRLPSIYHVALVSRSVADSTITISDIYSIVKRRDADKVGLDYHQSRWLEFFEDIDFLRLSSGFKIIFELYCMNVTRKKHDQSFGTPKRRTPMKKLFYDSPNKFTDSPILLSNVGTFDLTSKDFIVNNNDIIEKNLHLPNSNPFDGCVKLDGSMSFSVASSCEGRLNVYMEVGKIGSWAFRDCQLSHTGLIVGKKSEQTGIIEEVDANIPFFTMKESANLCDFQVCAKANSFSVLLIDQEIKEKMAINRRISTDGKRKYRFLAAAESQDDACRWCKCINDCNDSFKDWSKNID 719          
BLAST of SMED30011704 vs. Planmine SMEST
Match: SMESG000008014.1 (SMESG000008014.1)

HSP 1 Score: 356.681 bits (914), Expect = 4.184e-112
Identity = 207/471 (43.95%), Postives = 294/471 (62.42%), Query Frame = 1
Query:  811 DVESLP--------EKYSKIERIKPRRSLSFNEKVNVIEI-SDSDSSENLA-NTPGKNLEFNAKLSYITPDNLQENFESSDEPTICFEMEELKQRSWHLIKDLEAFSSTLSYTPGNLRHVEKCIDIVKNNLLYASVVERIHCQHNSNIGIIADVDIHGVRFSIKDNYVFFNYKSLLARGYDSKISRLPSIYHVALVSRSVAXXXXXXXXXXXXVKRRDADKVGLDYHQSRWLEFFEDIDFLRLSSGFKIIFELYCMNVTRKKHDQSFGTPKRRTPMKK---LFYDSPNKFTDSPILLSNVGTFDLTSKDFIVNNNDIIEKNLHLPNSNPFDGCVKLDGSMSFSVASSCEGRLNVYMEVGKIGSWAFRDCQLSHTGLIVGKKSEQTGIIEEVDANIPFFTMKESANLCDFQVCAKANSFSVLLIDQEIKEKMAINRRISTDGKRKYRFLAAAESQDDACRWCKCINDCNDSF 2184
            D E LP        +++++ E I    SLS  +++N  +I +D DSS N + N P +N   N   + +  +N  +NF  S+E  I  +M  LK++  HL+  L        +TP ++ H+EKC++I++NNL+Y+S+ E +H QH SN    AD  +  + FSIKD YVF+N+KSLL +G+  KI RL S+YHV LV RSV  S +  S+++SI+K+ DADK+ L    S+WL   + +DF RL   FK+ FELYCM+V  KKHD       +  P+KK   L +    K    P+L +N+G+ +LT ++ I N +  I+KN+ +  S+P DG VKL   +  S+ S C+G+L VY EV  IGSW+F DC+L+  G+I GKKS++TGIIE ++  I F  MK  ANLC     +KA+SFSVLL  +EI+ KM +NRRIS +G  KY FLAAA+S +DA RWCKCIN C DSF
Sbjct:  263 DNEQLPNTTDIGADKEHTEQEAINLHCSLSLKDELNTTKICADFDSSVNASENIPDENKTIND--TQLPTENSLDNFVVSEESIIICQMGHLKEKRLHLMNGLFV---NYRFTPVDVHHIEKCVEILRNNLMYSSLAESLHHQHYSNDSFHADAYVRNIMFSIKDEYVFYNFKSLLDKGFGDKIKRLSSVYHVILVGRSVGGSRVYESEVFSIIKKCDADKIKLYNGMSQWLRMIKTLDFTRLPINFKLNFELYCMHVNPKKHD-------KEKPLKKSTQLLFRRTTKIA-GPVLFTNIGSLNLTPREIIKNGS--IQKNIIVHQSSPLDGFVKLTSLV--SLISGCQGKLEVYSEVNNIGSWSFMDCELTTKGIIGGKKSKETGIIENINFEISFNVMKNLANLCCSSAFSKAHSFSVLLNKEEIEGKMPVNRRISINGTEKYTFLAAAKSPEDALRWCKCINLCRDSF 716          

HSP 2 Score: 82.8037 bits (203), Expect = 4.907e-16
Identity = 52/116 (44.83%), Postives = 77/116 (66.38%), Query Frame = 1
Query:   49 IMDESFDISKIVYASQNSKITLKTKQKLDELAAQVAFWNSEVYQMSSNKENRIVNL----SKPVISNVKKSFVFSNDKFSANSVKFPLTESRSGNRKSMLDCKGSVREKKAYFESL 384
            +++++FD+ K++YA+ NSKITL +K+KLD+L AQV  WNSE    SSNKEN  +      +KP +S+ K S + SN+     +V   LTE+ + ++   LD K SV+ +KAYFESL
Sbjct:    1 MLNKTFDLDKVIYANNNSKITLSSKKKLDKLTAQVDMWNSEFTVKSSNKENLPILTAKINTKPRVSSSKNSCLLSNNNDIMPTV-ITLTET-TKSKSFKLDGKLSVKNRKAYFESL 114          
BLAST of SMED30011704 vs. Planmine SMEST
Match: SMESG000011901.1 (SMESG000011901.1)

HSP 1 Score: 286.189 bits (731), Expect = 9.793e-86
Identity = 186/463 (40.17%), Postives = 272/463 (58.75%), Query Frame = 1
Query:  781 FEYGKMTKENDVESLPEKYSKIERIKPRRSLSFNEKVNVIEI-SDSDSSENLANTPGKNLEFNAKLSYITPDNLQENFESSDEPTICFEMEELKQRSWHLIKDLEAFSSTLSYTPGNLRHVEKCIDIVKNNLLYASVVERIHCQHNSNIGIIADVDIHGVRFSIKDNYVFFNYKSLLARGYDSKISRLPSIYHVALVSRSVAXXXXXXXXXXXXVKRRDADKVGLDYHQSRWLEFFEDIDFLRLSSGFKIIFELYCMNVTRKKHDQSFGTPKRRTPMKKLFYDSPNKFTDSPILLSNVGTFDLTSKDFIVNNNDIIEKNLHLPNSNPFDGCVKLDGSMSFSVASSCEGRLNVYMEVGKIGSWAFRDCQLSHTGLIVGKKSEQTGIIEEVDANIPFFTMKESANLCDFQVCAKANSFSVLLIDQEIKEKMAINRRISTDGKRKYRFLAAAESQDDACRWCKCIN 2166
             E  +++ E   +SL  K +KI+R K RRS+SF EK++VIEI SD DS           L+F+              +E  DE  +  ++E++KQ+S  L +++   S+     PGN  HV KC++I+KNN LY S+VER H   NS    ++ VD+  V+ S+KD YVF+N  SL+  GY   ++ L S++HV LV+RSV  S I  S ++SIVKR++A ++ L    SRWLE F  ++F+ L   FK+ FELYCM+VT KK+        R    K L+ +SP    +S IL +N+G+F+LTS D  ++    IEK ++LP S+P DG VKL   + F       G LN+Y EV    SW+ + C++   G++ GKK E  GI+ E+D  IPF  MK  ANLCDF    K NS +V L  +E  E+  IN++I+T G+ +Y F+ A++S++D  +WC  IN
Sbjct:  265 LECEQISNEKVAKSL-SKSTKIKRKKVRRSISFIEKISVIEIGSDLDS-----------LKFDI-------------YERKDEERLRLQLEDVKQKSLDLAREICTLSNI--NKPGNFYHVSKCVEILKNNNLYTSLVERQHLGKNSK-DFLSHVDLCNVQISVKDEYVFYNLDSLIKLGYGEHLNNLSSLFHVILVARSVGGSDIMESSVFSIVKRQEAKQIKLHPGGSRWLEMFHTMNFIELPILFKLNFELYCMHVTPKKY--CINVVDR---FKSLYRNSPQNI-ESTILFTNIGSFNLTSDD--IHKKKFIEKRMNLPQSSPLDGNVKLISILRFG------GFLNIYREVNGRASWSLKYCEVISDGIVCGKKPE-NGIMMEIDIVIPFSLMKGPANLCDF---LKPNSLAVYLEVRESDEEKFINQKITTSGEPEYIFMGASDSEEDVSKWCNVIN 681          
BLAST of SMED30011704 vs. Planmine SMEST
Match: SMESG000011901.1 (SMESG000011901.1)

HSP 1 Score: 285.804 bits (730), Expect = 1.025e-85
Identity = 186/463 (40.17%), Postives = 272/463 (58.75%), Query Frame = 1
Query:  781 FEYGKMTKENDVESLPEKYSKIERIKPRRSLSFNEKVNVIEI-SDSDSSENLANTPGKNLEFNAKLSYITPDNLQENFESSDEPTICFEMEELKQRSWHLIKDLEAFSSTLSYTPGNLRHVEKCIDIVKNNLLYASVVERIHCQHNSNIGIIADVDIHGVRFSIKDNYVFFNYKSLLARGYDSKISRLPSIYHVALVSRSVAXXXXXXXXXXXXVKRRDADKVGLDYHQSRWLEFFEDIDFLRLSSGFKIIFELYCMNVTRKKHDQSFGTPKRRTPMKKLFYDSPNKFTDSPILLSNVGTFDLTSKDFIVNNNDIIEKNLHLPNSNPFDGCVKLDGSMSFSVASSCEGRLNVYMEVGKIGSWAFRDCQLSHTGLIVGKKSEQTGIIEEVDANIPFFTMKESANLCDFQVCAKANSFSVLLIDQEIKEKMAINRRISTDGKRKYRFLAAAESQDDACRWCKCIN 2166
             E  +++ E   +SL  K +KI+R K RRS+SF EK++VIEI SD DS           L+F+              +E  DE  +  ++E++KQ+S  L +++   S+     PGN  HV KC++I+KNN LY S+VER H   NS    ++ VD+  V+ S+KD YVF+N  SL+  GY   ++ L S++HV LV+RSV  S I  S ++SIVKR++A ++ L    SRWLE F  ++F+ L   FK+ FELYCM+VT KK+        R    K L+ +SP    +S IL +N+G+F+LTS D  ++    IEK ++LP S+P DG VKL   + F       G LN+Y EV    SW+ + C++   G++ GKK E  GI+ E+D  IPF  MK  ANLCDF    K NS +V L  +E  E+  IN++I+T G+ +Y F+ A++S++D  +WC  IN
Sbjct:  261 LECEQISNEKVAKSL-SKSTKIKRKKVRRSISFIEKISVIEIGSDLDS-----------LKFDI-------------YERKDEERLRLQLEDVKQKSLDLAREICTLSNI--NKPGNFYHVSKCVEILKNNNLYTSLVERQHLGKNSK-DFLSHVDLCNVQISVKDEYVFYNLDSLIKLGYGEHLNNLSSLFHVILVARSVGGSDIMESSVFSIVKRQEAKQIKLHPGGSRWLEMFHTMNFIELPILFKLNFELYCMHVTPKKY--CINVVDR---FKSLYRNSPQNI-ESTILFTNIGSFNLTSDD--IHKKKFIEKRMNLPQSSPLDGNVKLISILRFG------GFLNIYREVNGRASWSLKYCEVISDGIVCGKKPE-NGIMMEIDIVIPFSLMKGPANLCDF---LKPNSLAVYLEVRESDEEKFINQKITTSGEPEYIFMGASDSEEDVSKWCNVIN 677          
BLAST of SMED30011704 vs. Planmine SMEST
Match: SMESG000011905.1 (SMESG000011905.1)

HSP 1 Score: 281.567 bits (719), Expect = 1.292e-83
Identity = 188/475 (39.58%), Postives = 276/475 (58.11%), Query Frame = 1
Query:  766 FDEFIFEYGKMTKENDVESLPEKYSKIERIKPRRSLSFNEKVNVIEI-SDSDSSENLANTPGKNLEFNAKLSYITPDNLQENFESSDEPTICFEMEELKQRSWHLIKDLEAFSSTLSYTPGNLRHVEKCIDIVKNNLLYASVVERIHCQHNSNIGIIADVDIHGVRFSIKDNYVFFNYKSLLARGYDSKISRLPSIYHVALVSRSVAXXXXXXXXXXXXVKRRDADKVGLDYHQSRWLEFFEDIDFLRLSSGFKIIFELYCMNVTRKKHDQSFGTPKRRTPMKKLFYDSPNKFTDSPILLSNVGTFDLTSKDFIVNNNDIIEKNLHLPNSNPFDGCVKLDGSMSFSVASSCEGRLNVYMEVGKIGSWAFRDCQLSHTGLIVGKKSEQTGIIEEVDANIPFFTMKESANLCDFQVCAKANSFSVLLIDQEIKEKMAINRRISTDGKRKYRFLAAAESQDDACRWCKCIN---DCND 2178
            F E   E  +++ E   +SL  K +KI+R K RRS+SF EK++VIEI SD DS           L+F+              +E  DE  +  ++E++KQ+S  L +++   S+     PGN  HV KC++I+KNN LY S+VER H   NS    ++ VD+  V+ S+KD YVF+N  SL+  GY   ++ L S++HV LV+RSV  S I  S ++SIVKR++A ++ L    SRWLE F  ++F+ L   FK+ FELYCM+VT KK+        R    K L+ +SP    +S IL +N+G+F+LTS D  ++    IEK ++LP S+P DG VKL   + F       G LN+Y EV    SW+ + C++   G++ GKK  + GI++E+D  IPF  MK  ANL DF    K NS +V L  + + E   I+++I+T G+ +Y F+ A++S+ DA RWC  IN   +C D
Sbjct:  306 FSESKLECEQISNEKVAKSL-SKSTKIKRKKVRRSISFIEKISVIEIGSDLDS-----------LKFDI-------------YERKDEERLRLQLEDVKQKSLDLAREICTLSNI--NKPGNFYHVSKCVEILKNNNLYTSLVERQHLGKNSK-DFLSHVDLCNVQISVKDEYVFYNLDSLIKLGYGEHLNNLSSLFHVILVARSVGGSDIMESSVFSIVKRQEAKQIKLHPGGSRWLEMFHTMNFIELPILFKLNFELYCMHVTPKKY--CINVVDR---FKSLYRNSPQNI-ESTILFTNIGSFNLTSDD--IHKKKFIEKRMNLPQSSPLDGNVKLISILRFG------GFLNIYREVNGRASWSLKYCEVISDGIVCGKKP-KNGIMKEIDIVIPFSLMKGPANLFDF---LKPNSLAVCLEVKRVDEIKFIDQKITTSGEPEYIFIGASDSEQDASRWCDFINQFLECAD 734          
The following BLAST results are available for this feature:
BLAST of SMED30011704 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011704 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011704 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011704 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011704 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011704 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011704 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011704 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011704 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011704 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011704 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011704 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011704 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011704 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000063079.10.000e+0100.00SMESG000063079.1[more]
SMESG000008014.14.184e-11243.95SMESG000008014.1[more]
SMESG000011901.19.793e-8640.17SMESG000011901.1[more]
SMESG000011901.11.025e-8540.17SMESG000011901.1[more]
SMESG000011905.11.292e-8339.58SMESG000011905.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011704 ID=SMED30011704|Name=SMED30011704|organism=Schmidtea mediterranea sexual|type=transcript|length=2277bp
ACTTCTTATTAAGATTCAAATAATAAAGAAATTTTTTAATAAATATTTAT
AATGGATGAAAGTTTTGACATTAGTAAAATTGTGTATGCCAGTCAGAATT
CAAAAATTACTCTAAAAACAAAGCAAAAGTTAGATGAATTAGCTGCTCAA
GTTGCATTTTGGAATTCGGAAGTTTATCAGATGTCATCCAACAAAGAAAA
TCGAATAGTTAATTTATCTAAGCCAGTAATCTCCAATGTTAAGAAAAGTT
TCGTGTTTTCAAATGATAAATTTTCAGCTAATTCTGTCAAATTTCCTTTG
ACAGAATCAAGAAGTGGTAATAGGAAAAGTATGTTGGATTGTAAAGGATC
AGTGAGAGAAAAGAAGGCATATTTTGAATCTTTGACTCAAACAACACCAA
AAGGTAAAATACCGATTTTCAAAGTTCAGCAGTCATTTAAAAATATAGAA
TATCCTAAAATTTTTGAAGTTTCTTCAAGAAATGCTAATGAATCGGTCAT
CACCGAAGAACAAAACAAAGATGATAATAACATAAGTGTCGAAGACATTC
TTCCTCCTCCTCCTTATATATCCGATGAATCTCAATATGGTGACGAAGAA
ATTGTTGTTACGGACAAAGGTCACCGCGAATCTTTGAAAAATGAATCCAC
TGTAGTATCTGAGTTGGATGCATCATGGAATAATGTTGACACACAACTTA
ACAATATTAGTTCAGATTTAACTCGTGTTTCTTCACTTTCTTCTTCACAA
AGTATTGGATCGTTGTTTGATGAATTTATTTTTGAATATGGAAAAATGAC
AAAAGAAAATGACGTGGAATCTCTCCCTGAAAAATACTCTAAAATCGAAC
GAATAAAACCTCGTCGATCGTTAAGTTTTAATGAAAAAGTCAATGTTATT
GAAATATCTGATTCTGATTCGTCGGAAAATTTAGCTAATACTCCGGGAAA
AAACCTAGAATTTAATGCCAAATTATCATATATAACTCCTGACAACTTGC
AAGAAAATTTTGAATCAAGTGATGAGCCAACTATCTGTTTTGAAATGGAA
GAATTAAAACAAAGAAGTTGGCATTTAATCAAAGATCTCGAAGCATTTTC
GTCAACTCTTAGCTACACACCCGGAAATTTGCGTCACGTTGAGAAATGTA
TCGATATTGTCAAAAATAATTTGTTGTATGCATCTGTGGTTGAAAGAATC
CATTGCCAACACAATTCAAATATCGGCATTATTGCTGATGTTGATATTCA
TGGCGTTCGGTTCAGCATCAAGGATAACTATGTGTTTTTCAATTATAAAT
CCCTACTTGCAAGGGGATATGACAGCAAAATCAGTCGTTTACCTAGCATT
TATCATGTCGCTTTGGTATCTCGCTCAGTTGCAGATTCCACAATAACCAT
ATCTGATATATATTCAATTGTAAAGCGCCGAGATGCTGATAAAGTTGGAT
TGGATTATCACCAATCAAGGTGGCTTGAATTTTTCGAAGATATTGATTTT
CTTCGATTGTCATCGGGATTCAAGATAATTTTTGAGCTCTACTGCATGAA
TGTCACACGGAAAAAACACGATCAATCATTTGGAACACCAAAAAGACGGA
CTCCAATGAAAAAGTTATTTTACGATTCTCCTAATAAATTTACCGACAGC
CCAATCCTGCTATCGAATGTCGGCACATTCGATTTGACATCGAAGGACTT
TATAGTGAATAATAATGATATTATTGAGAAGAATTTACACTTGCCTAATA
GCAATCCATTTGATGGGTGCGTGAAATTGGATGGGTCTATGTCATTCAGT
GTTGCATCGAGTTGTGAAGGCCGACTGAATGTATACATGGAAGTGGGTAA
AATCGGTTCGTGGGCCTTCAGGGATTGCCAGCTATCTCACACGGGCCTTA
TAGTTGGAAAGAAGTCCGAACAAACTGGAATCATTGAAGAAGTCGATGCA
AACATTCCATTTTTCACAATGAAAGAATCCGCCAATCTCTGTGACTTTCA
AGTTTGTGCCAAAGCGAATTCATTTTCAGTGCTTCTAATTGATCAAGAAA
TCAAAGAAAAGATGGCAATCAATCGAAGAATATCGACAGATGGCAAACGG
AAGTATCGATTTTTAGCAGCTGCTGAATCGCAAGACGACGCCTGCCGATG
GTGCAAATGCATTAATGATTGCAATGACTCGTTCAAAGATTGGTCAAAGA
ATATTGATTTATTGTAGAAAATTATTATTGTGGTTTTGATTTGTCTTTTT
CACAGCTTATTTCGATATGTTCCGATG
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protein sequence of SMED30011704-orf-1

>SMED30011704-orf-1 ID=SMED30011704-orf-1|Name=SMED30011704-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=722bp
MDESFDISKIVYASQNSKITLKTKQKLDELAAQVAFWNSEVYQMSSNKEN
RIVNLSKPVISNVKKSFVFSNDKFSANSVKFPLTESRSGNRKSMLDCKGS
VREKKAYFESLTQTTPKGKIPIFKVQQSFKNIEYPKIFEVSSRNANESVI
TEEQNKDDNNISVEDILPPPPYISDESQYGDEEIVVTDKGHRESLKNEST
VVSELDASWNNVDTQLNNISSDLTRVSSLSSSQSIGSLFDEFIFEYGKMT
KENDVESLPEKYSKIERIKPRRSLSFNEKVNVIEISDSDSSENLANTPGK
NLEFNAKLSYITPDNLQENFESSDEPTICFEMEELKQRSWHLIKDLEAFS
STLSYTPGNLRHVEKCIDIVKNNLLYASVVERIHCQHNSNIGIIADVDIH
GVRFSIKDNYVFFNYKSLLARGYDSKISRLPSIYHVALVSRSVADSTITI
SDIYSIVKRRDADKVGLDYHQSRWLEFFEDIDFLRLSSGFKIIFELYCMN
VTRKKHDQSFGTPKRRTPMKKLFYDSPNKFTDSPILLSNVGTFDLTSKDF
IVNNNDIIEKNLHLPNSNPFDGCVKLDGSMSFSVASSCEGRLNVYMEVGK
IGSWAFRDCQLSHTGLIVGKKSEQTGIIEEVDANIPFFTMKESANLCDFQ
VCAKANSFSVLLIDQEIKEKMAINRRISTDGKRKYRFLAAAESQDDACRW
CKCINDCNDSFKDWSKNIDLL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000420parapharyngeal region
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR011993PH-like_dom_sf
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011993PH-like domain superfamilyGENE3DG3DSA:2.30.29.30coord: 587..715
e-value: 1.0E-6
score: 31.1