SMED30011704
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30011704 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Alignments
SMED30011704 aligns in the following genomic locations:
Homology
BLAST of SMED30011704 vs. Planmine SMEST
Match: SMESG000063079.1 (SMESG000063079.1) HSP 1 Score: 1402.88 bits (3630), Expect = 0.000e+0 Identity = 719/719 (100.00%), Postives = 719/719 (100.00%), Query Frame = 1 Query: 52 MDESFDISKIVYASQNSKITLKTKQKLDELAAQVAFWNSEVYQMSSNKENRIVNLSKPVISNVKKSFVFSNDKFSANSVKFPLTESRSGNRKSMLDCKGSVREKKAYFESLTQTTPKGKIPIFKVQQSFKNIEYPKIFEVSSRNANESVITEEQNKDDNNISVEDILPPPPYISDESQYGDEEIVVTDKGHRESLKNESTVVSELDASWNNVDTQLNNIXXXXXXXXXXXXXXXXXXXFDEFIFEYGKMTKENDVESLPEKYSKIERIKPRRSLSFNEKVNVIEISDSDSSENLANTPGKNLEFNAKLSYITPDNLQENFESSDEPTICFEMEELKQRSWHLIKDLEAFSSTLSYTPGNLRHVEKCIDIVKNNLLYASVVERIHCQHNSNIGIIADVDIHGVRFSIKDNYVFFNYKSLLARGYDSKISRLPSIYHVALVSRSVAXXXXXXXXXXXXVKRRDADKVGLDYHQSRWLEFFEDIDFLRLSSGFKIIFELYCMNVTRKKHDQSFGTPKRRTPMKKLFYDSPNKFTDSPILLSNVGTFDLTSKDFIVNNNDIIEKNLHLPNSNPFDGCVKLDGSMSFSVASSCEGRLNVYMEVGKIGSWAFRDCQLSHTGLIVGKKSEQTGIIEEVDANIPFFTMKESANLCDFQVCAKANSFSVLLIDQEIKEKMAINRRISTDGKRKYRFLAAAESQDDACRWCKCINDCNDSFKDWSKNID 2208 MDESFDISKIVYASQNSKITLKTKQKLDELAAQVAFWNSEVYQMSSNKENRIVNLSKPVISNVKKSFVFSNDKFSANSVKFPLTESRSGNRKSMLDCKGSVREKKAYFESLTQTTPKGKIPIFKVQQSFKNIEYPKIFEVSSRNANESVITEEQNKDDNNISVEDILPPPPYISDESQYGDEEIVVTDKGHRESLKNESTVVSELDASWNNVDTQLNNISSDLTRVSSLSSSQSIGSLFDEFIFEYGKMTKENDVESLPEKYSKIERIKPRRSLSFNEKVNVIEISDSDSSENLANTPGKNLEFNAKLSYITPDNLQENFESSDEPTICFEMEELKQRSWHLIKDLEAFSSTLSYTPGNLRHVEKCIDIVKNNLLYASVVERIHCQHNSNIGIIADVDIHGVRFSIKDNYVFFNYKSLLARGYDSKISRLPSIYHVALVSRSVADSTITISDIYSIVKRRDADKVGLDYHQSRWLEFFEDIDFLRLSSGFKIIFELYCMNVTRKKHDQSFGTPKRRTPMKKLFYDSPNKFTDSPILLSNVGTFDLTSKDFIVNNNDIIEKNLHLPNSNPFDGCVKLDGSMSFSVASSCEGRLNVYMEVGKIGSWAFRDCQLSHTGLIVGKKSEQTGIIEEVDANIPFFTMKESANLCDFQVCAKANSFSVLLIDQEIKEKMAINRRISTDGKRKYRFLAAAESQDDACRWCKCINDCNDSFKDWSKNID Sbjct: 1 MDESFDISKIVYASQNSKITLKTKQKLDELAAQVAFWNSEVYQMSSNKENRIVNLSKPVISNVKKSFVFSNDKFSANSVKFPLTESRSGNRKSMLDCKGSVREKKAYFESLTQTTPKGKIPIFKVQQSFKNIEYPKIFEVSSRNANESVITEEQNKDDNNISVEDILPPPPYISDESQYGDEEIVVTDKGHRESLKNESTVVSELDASWNNVDTQLNNISSDLTRVSSLSSSQSIGSLFDEFIFEYGKMTKENDVESLPEKYSKIERIKPRRSLSFNEKVNVIEISDSDSSENLANTPGKNLEFNAKLSYITPDNLQENFESSDEPTICFEMEELKQRSWHLIKDLEAFSSTLSYTPGNLRHVEKCIDIVKNNLLYASVVERIHCQHNSNIGIIADVDIHGVRFSIKDNYVFFNYKSLLARGYDSKISRLPSIYHVALVSRSVADSTITISDIYSIVKRRDADKVGLDYHQSRWLEFFEDIDFLRLSSGFKIIFELYCMNVTRKKHDQSFGTPKRRTPMKKLFYDSPNKFTDSPILLSNVGTFDLTSKDFIVNNNDIIEKNLHLPNSNPFDGCVKLDGSMSFSVASSCEGRLNVYMEVGKIGSWAFRDCQLSHTGLIVGKKSEQTGIIEEVDANIPFFTMKESANLCDFQVCAKANSFSVLLIDQEIKEKMAINRRISTDGKRKYRFLAAAESQDDACRWCKCINDCNDSFKDWSKNID 719
BLAST of SMED30011704 vs. Planmine SMEST
Match: SMESG000008014.1 (SMESG000008014.1) HSP 1 Score: 356.681 bits (914), Expect = 4.184e-112 Identity = 207/471 (43.95%), Postives = 294/471 (62.42%), Query Frame = 1 Query: 811 DVESLP--------EKYSKIERIKPRRSLSFNEKVNVIEI-SDSDSSENLA-NTPGKNLEFNAKLSYITPDNLQENFESSDEPTICFEMEELKQRSWHLIKDLEAFSSTLSYTPGNLRHVEKCIDIVKNNLLYASVVERIHCQHNSNIGIIADVDIHGVRFSIKDNYVFFNYKSLLARGYDSKISRLPSIYHVALVSRSVAXXXXXXXXXXXXVKRRDADKVGLDYHQSRWLEFFEDIDFLRLSSGFKIIFELYCMNVTRKKHDQSFGTPKRRTPMKK---LFYDSPNKFTDSPILLSNVGTFDLTSKDFIVNNNDIIEKNLHLPNSNPFDGCVKLDGSMSFSVASSCEGRLNVYMEVGKIGSWAFRDCQLSHTGLIVGKKSEQTGIIEEVDANIPFFTMKESANLCDFQVCAKANSFSVLLIDQEIKEKMAINRRISTDGKRKYRFLAAAESQDDACRWCKCINDCNDSF 2184 D E LP +++++ E I SLS +++N +I +D DSS N + N P +N N + + +N +NF S+E I +M LK++ HL+ L +TP ++ H+EKC++I++NNL+Y+S+ E +H QH SN AD + + FSIKD YVF+N+KSLL +G+ KI RL S+YHV LV RSV S + S+++SI+K+ DADK+ L S+WL + +DF RL FK+ FELYCM+V KKHD + P+KK L + K P+L +N+G+ +LT ++ I N + I+KN+ + S+P DG VKL + S+ S C+G+L VY EV IGSW+F DC+L+ G+I GKKS++TGIIE ++ I F MK ANLC +KA+SFSVLL +EI+ KM +NRRIS +G KY FLAAA+S +DA RWCKCIN C DSF Sbjct: 263 DNEQLPNTTDIGADKEHTEQEAINLHCSLSLKDELNTTKICADFDSSVNASENIPDENKTIND--TQLPTENSLDNFVVSEESIIICQMGHLKEKRLHLMNGLFV---NYRFTPVDVHHIEKCVEILRNNLMYSSLAESLHHQHYSNDSFHADAYVRNIMFSIKDEYVFYNFKSLLDKGFGDKIKRLSSVYHVILVGRSVGGSRVYESEVFSIIKKCDADKIKLYNGMSQWLRMIKTLDFTRLPINFKLNFELYCMHVNPKKHD-------KEKPLKKSTQLLFRRTTKIA-GPVLFTNIGSLNLTPREIIKNGS--IQKNIIVHQSSPLDGFVKLTSLV--SLISGCQGKLEVYSEVNNIGSWSFMDCELTTKGIIGGKKSKETGIIENINFEISFNVMKNLANLCCSSAFSKAHSFSVLLNKEEIEGKMPVNRRISINGTEKYTFLAAAKSPEDALRWCKCINLCRDSF 716 HSP 2 Score: 82.8037 bits (203), Expect = 4.907e-16 Identity = 52/116 (44.83%), Postives = 77/116 (66.38%), Query Frame = 1 Query: 49 IMDESFDISKIVYASQNSKITLKTKQKLDELAAQVAFWNSEVYQMSSNKENRIVNL----SKPVISNVKKSFVFSNDKFSANSVKFPLTESRSGNRKSMLDCKGSVREKKAYFESL 384 +++++FD+ K++YA+ NSKITL +K+KLD+L AQV WNSE SSNKEN + +KP +S+ K S + SN+ +V LTE+ + ++ LD K SV+ +KAYFESL Sbjct: 1 MLNKTFDLDKVIYANNNSKITLSSKKKLDKLTAQVDMWNSEFTVKSSNKENLPILTAKINTKPRVSSSKNSCLLSNNNDIMPTV-ITLTET-TKSKSFKLDGKLSVKNRKAYFESL 114
BLAST of SMED30011704 vs. Planmine SMEST
Match: SMESG000011901.1 (SMESG000011901.1) HSP 1 Score: 286.189 bits (731), Expect = 9.793e-86 Identity = 186/463 (40.17%), Postives = 272/463 (58.75%), Query Frame = 1 Query: 781 FEYGKMTKENDVESLPEKYSKIERIKPRRSLSFNEKVNVIEI-SDSDSSENLANTPGKNLEFNAKLSYITPDNLQENFESSDEPTICFEMEELKQRSWHLIKDLEAFSSTLSYTPGNLRHVEKCIDIVKNNLLYASVVERIHCQHNSNIGIIADVDIHGVRFSIKDNYVFFNYKSLLARGYDSKISRLPSIYHVALVSRSVAXXXXXXXXXXXXVKRRDADKVGLDYHQSRWLEFFEDIDFLRLSSGFKIIFELYCMNVTRKKHDQSFGTPKRRTPMKKLFYDSPNKFTDSPILLSNVGTFDLTSKDFIVNNNDIIEKNLHLPNSNPFDGCVKLDGSMSFSVASSCEGRLNVYMEVGKIGSWAFRDCQLSHTGLIVGKKSEQTGIIEEVDANIPFFTMKESANLCDFQVCAKANSFSVLLIDQEIKEKMAINRRISTDGKRKYRFLAAAESQDDACRWCKCIN 2166 E +++ E +SL K +KI+R K RRS+SF EK++VIEI SD DS L+F+ +E DE + ++E++KQ+S L +++ S+ PGN HV KC++I+KNN LY S+VER H NS ++ VD+ V+ S+KD YVF+N SL+ GY ++ L S++HV LV+RSV S I S ++SIVKR++A ++ L SRWLE F ++F+ L FK+ FELYCM+VT KK+ R K L+ +SP +S IL +N+G+F+LTS D ++ IEK ++LP S+P DG VKL + F G LN+Y EV SW+ + C++ G++ GKK E GI+ E+D IPF MK ANLCDF K NS +V L +E E+ IN++I+T G+ +Y F+ A++S++D +WC IN Sbjct: 265 LECEQISNEKVAKSL-SKSTKIKRKKVRRSISFIEKISVIEIGSDLDS-----------LKFDI-------------YERKDEERLRLQLEDVKQKSLDLAREICTLSNI--NKPGNFYHVSKCVEILKNNNLYTSLVERQHLGKNSK-DFLSHVDLCNVQISVKDEYVFYNLDSLIKLGYGEHLNNLSSLFHVILVARSVGGSDIMESSVFSIVKRQEAKQIKLHPGGSRWLEMFHTMNFIELPILFKLNFELYCMHVTPKKY--CINVVDR---FKSLYRNSPQNI-ESTILFTNIGSFNLTSDD--IHKKKFIEKRMNLPQSSPLDGNVKLISILRFG------GFLNIYREVNGRASWSLKYCEVISDGIVCGKKPE-NGIMMEIDIVIPFSLMKGPANLCDF---LKPNSLAVYLEVRESDEEKFINQKITTSGEPEYIFMGASDSEEDVSKWCNVIN 681
BLAST of SMED30011704 vs. Planmine SMEST
Match: SMESG000011901.1 (SMESG000011901.1) HSP 1 Score: 285.804 bits (730), Expect = 1.025e-85 Identity = 186/463 (40.17%), Postives = 272/463 (58.75%), Query Frame = 1 Query: 781 FEYGKMTKENDVESLPEKYSKIERIKPRRSLSFNEKVNVIEI-SDSDSSENLANTPGKNLEFNAKLSYITPDNLQENFESSDEPTICFEMEELKQRSWHLIKDLEAFSSTLSYTPGNLRHVEKCIDIVKNNLLYASVVERIHCQHNSNIGIIADVDIHGVRFSIKDNYVFFNYKSLLARGYDSKISRLPSIYHVALVSRSVAXXXXXXXXXXXXVKRRDADKVGLDYHQSRWLEFFEDIDFLRLSSGFKIIFELYCMNVTRKKHDQSFGTPKRRTPMKKLFYDSPNKFTDSPILLSNVGTFDLTSKDFIVNNNDIIEKNLHLPNSNPFDGCVKLDGSMSFSVASSCEGRLNVYMEVGKIGSWAFRDCQLSHTGLIVGKKSEQTGIIEEVDANIPFFTMKESANLCDFQVCAKANSFSVLLIDQEIKEKMAINRRISTDGKRKYRFLAAAESQDDACRWCKCIN 2166 E +++ E +SL K +KI+R K RRS+SF EK++VIEI SD DS L+F+ +E DE + ++E++KQ+S L +++ S+ PGN HV KC++I+KNN LY S+VER H NS ++ VD+ V+ S+KD YVF+N SL+ GY ++ L S++HV LV+RSV S I S ++SIVKR++A ++ L SRWLE F ++F+ L FK+ FELYCM+VT KK+ R K L+ +SP +S IL +N+G+F+LTS D ++ IEK ++LP S+P DG VKL + F G LN+Y EV SW+ + C++ G++ GKK E GI+ E+D IPF MK ANLCDF K NS +V L +E E+ IN++I+T G+ +Y F+ A++S++D +WC IN Sbjct: 261 LECEQISNEKVAKSL-SKSTKIKRKKVRRSISFIEKISVIEIGSDLDS-----------LKFDI-------------YERKDEERLRLQLEDVKQKSLDLAREICTLSNI--NKPGNFYHVSKCVEILKNNNLYTSLVERQHLGKNSK-DFLSHVDLCNVQISVKDEYVFYNLDSLIKLGYGEHLNNLSSLFHVILVARSVGGSDIMESSVFSIVKRQEAKQIKLHPGGSRWLEMFHTMNFIELPILFKLNFELYCMHVTPKKY--CINVVDR---FKSLYRNSPQNI-ESTILFTNIGSFNLTSDD--IHKKKFIEKRMNLPQSSPLDGNVKLISILRFG------GFLNIYREVNGRASWSLKYCEVISDGIVCGKKPE-NGIMMEIDIVIPFSLMKGPANLCDF---LKPNSLAVYLEVRESDEEKFINQKITTSGEPEYIFMGASDSEEDVSKWCNVIN 677
BLAST of SMED30011704 vs. Planmine SMEST
Match: SMESG000011905.1 (SMESG000011905.1) HSP 1 Score: 281.567 bits (719), Expect = 1.292e-83 Identity = 188/475 (39.58%), Postives = 276/475 (58.11%), Query Frame = 1 Query: 766 FDEFIFEYGKMTKENDVESLPEKYSKIERIKPRRSLSFNEKVNVIEI-SDSDSSENLANTPGKNLEFNAKLSYITPDNLQENFESSDEPTICFEMEELKQRSWHLIKDLEAFSSTLSYTPGNLRHVEKCIDIVKNNLLYASVVERIHCQHNSNIGIIADVDIHGVRFSIKDNYVFFNYKSLLARGYDSKISRLPSIYHVALVSRSVAXXXXXXXXXXXXVKRRDADKVGLDYHQSRWLEFFEDIDFLRLSSGFKIIFELYCMNVTRKKHDQSFGTPKRRTPMKKLFYDSPNKFTDSPILLSNVGTFDLTSKDFIVNNNDIIEKNLHLPNSNPFDGCVKLDGSMSFSVASSCEGRLNVYMEVGKIGSWAFRDCQLSHTGLIVGKKSEQTGIIEEVDANIPFFTMKESANLCDFQVCAKANSFSVLLIDQEIKEKMAINRRISTDGKRKYRFLAAAESQDDACRWCKCIN---DCND 2178 F E E +++ E +SL K +KI+R K RRS+SF EK++VIEI SD DS L+F+ +E DE + ++E++KQ+S L +++ S+ PGN HV KC++I+KNN LY S+VER H NS ++ VD+ V+ S+KD YVF+N SL+ GY ++ L S++HV LV+RSV S I S ++SIVKR++A ++ L SRWLE F ++F+ L FK+ FELYCM+VT KK+ R K L+ +SP +S IL +N+G+F+LTS D ++ IEK ++LP S+P DG VKL + F G LN+Y EV SW+ + C++ G++ GKK + GI++E+D IPF MK ANL DF K NS +V L + + E I+++I+T G+ +Y F+ A++S+ DA RWC IN +C D Sbjct: 306 FSESKLECEQISNEKVAKSL-SKSTKIKRKKVRRSISFIEKISVIEIGSDLDS-----------LKFDI-------------YERKDEERLRLQLEDVKQKSLDLAREICTLSNI--NKPGNFYHVSKCVEILKNNNLYTSLVERQHLGKNSK-DFLSHVDLCNVQISVKDEYVFYNLDSLIKLGYGEHLNNLSSLFHVILVARSVGGSDIMESSVFSIVKRQEAKQIKLHPGGSRWLEMFHTMNFIELPILFKLNFELYCMHVTPKKY--CINVVDR---FKSLYRNSPQNI-ESTILFTNIGSFNLTSDD--IHKKKFIEKRMNLPQSSPLDGNVKLISILRFG------GFLNIYREVNGRASWSLKYCEVISDGIVCGKKP-KNGIMKEIDIVIPFSLMKGPANLFDF---LKPNSLAVCLEVKRVDEIKFIDQKITTSGEPEYIFIGASDSEQDASRWCDFINQFLECAD 734 The following BLAST results are available for this feature:
BLAST of SMED30011704 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30011704 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30011704 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30011704 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30011704 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30011704 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30011704 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30011704 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30011704 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30011704 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30011704 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30011704 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30011704 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30011704 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30011704 ID=SMED30011704|Name=SMED30011704|organism=Schmidtea mediterranea sexual|type=transcript|length=2277bpback to top protein sequence of SMED30011704-orf-1 >SMED30011704-orf-1 ID=SMED30011704-orf-1|Name=SMED30011704-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=722bp MDESFDISKIVYASQNSKITLKTKQKLDELAAQVAFWNSEVYQMSSNKENback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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