Calcium-binding protein A
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30011700 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of Calcium-binding protein A vs. TrEMBL
Match: A0A1I8FHH7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 55.0694 bits (131), Expect = 2.548e-6 Identity = 24/67 (35.82%), Postives = 44/67 (65.67%), Query Frame = 2 Query: 68 KMFLAVDINKNGIISIEELRKHCSKCSNVDEAVEKFLKNFDTNRDREITYKEFADALGYPLNEKTLA 268 K FL++D + +G I+++EL+ +C K + + V+K+L FD +++ ITY E+ +ALG P + +A Sbjct: 13 KAFLSIDTDHSGEITVDELQSYCRKMNYQETFVQKWLMLFDADQNGRITYDEYCNALGLPPKKDVIA 79
BLAST of Calcium-binding protein A vs. TrEMBL
Match: A0A267FI43 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig004334g1 PE=4 SV=1) HSP 1 Score: 53.9138 bits (128), Expect = 6.158e-6 Identity = 24/67 (35.82%), Postives = 45/67 (67.16%), Query Frame = 2 Query: 68 KMFLAVDINKNGIISIEELRKHCSKCSNVDEAVEKFLKNFDTNRDREITYKEFADALGYPLNEKTLA 268 K FL++D +++G I+++EL+ +C K + + V+K+L FD +++ ITY E+ +ALG P + +A Sbjct: 13 KAFLSIDTDQSGEITVDELQSYCRKMNYQETFVQKWLMLFDADQNGRITYDEYCNALGLPPKKDVVA 79
BLAST of Calcium-binding protein A vs. TrEMBL
Match: A0A1I8IIW6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 53.9138 bits (128), Expect = 6.262e-6 Identity = 24/67 (35.82%), Postives = 45/67 (67.16%), Query Frame = 2 Query: 68 KMFLAVDINKNGIISIEELRKHCSKCSNVDEAVEKFLKNFDTNRDREITYKEFADALGYPLNEKTLA 268 K FL++D +++G I+++EL+ +C K + + V+K+L FD +++ ITY E+ +ALG P + +A Sbjct: 13 KAFLSIDTDQSGEITVDELQSYCRKMNYQETFVQKWLMLFDADQNGRITYDEYCNALGLPPKKDVVA 79
BLAST of Calcium-binding protein A vs. TrEMBL
Match: A0A267GN94 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig004334g2 PE=4 SV=1) HSP 1 Score: 53.5286 bits (127), Expect = 8.003e-6 Identity = 24/67 (35.82%), Postives = 44/67 (65.67%), Query Frame = 2 Query: 68 KMFLAVDINKNGIISIEELRKHCSKCSNVDEAVEKFLKNFDTNRDREITYKEFADALGYPLNEKTLA 268 K FL++D + +G I+++EL+ +C K + + V+K+L FD +++ ITY E+ +ALG P + +A Sbjct: 13 KAFLSIDTDHSGEITVDELQSYCRKMNYQETFVQKWLMLFDADQNGRITYDEYCNALGLPPKKDVIA 79
BLAST of Calcium-binding protein A vs. Planmine SMEST
Match: SMESG000048475.1 (SMESG000048475.1) HSP 1 Score: 162.155 bits (409), Expect = 1.845e-53 Identity = 77/78 (98.72%), Postives = 78/78 (100.00%), Query Frame = 2 Query: 47 MGDDMASKMFLAVDINKNGIISIEELRKHCSKCSNVDEAVEKFLKNFDTNRDREITYKEFADALGYPLNEKTLALFQK 280 MGDDMASKMFLAVDINKNGIIS+EELRKHCSKCSNVDEAVEKFLKNFDTNRDREITYKEFADALGYPLNEKTLALFQK Sbjct: 1 MGDDMASKMFLAVDINKNGIISVEELRKHCSKCSNVDEAVEKFLKNFDTNRDREITYKEFADALGYPLNEKTLALFQK 78
BLAST of Calcium-binding protein A vs. Planmine SMEST
Match: SMESG000002907.1 (SMESG000002907.1) HSP 1 Score: 119.013 bits (297), Expect = 2.546e-36 Identity = 51/78 (65.38%), Postives = 70/78 (89.74%), Query Frame = 2 Query: 47 MGDDMASKMFLAVDINKNGIISIEELRKHCSKCSNVDEAVEKFLKNFDTNRDREITYKEFADALGYPLNEKTLALFQK 280 MGDD+ASKMFL +D NKNG+ISI+EL++HCSK +NVDEA++ FLKNFD ++D+ +TY+EFA ALG P++EKTL++F+K Sbjct: 1 MGDDIASKMFLKIDTNKNGVISIDELKQHCSKTANVDEAIKTFLKNFDKDKDKNVTYEEFAKALGIPVSEKTLSVFKK 78
BLAST of Calcium-binding protein A vs. Planmine SMEST
Match: SMESG000045893.1 (SMESG000045893.1) HSP 1 Score: 61.6178 bits (148), Expect = 1.176e-13 Identity = 33/82 (40.24%), Postives = 51/82 (62.20%), Query Frame = 2 Query: 47 MGDDMASKMFLAVDINKNGIISIEELRKHCSKCSNVDEAVEK----FLKNFDTNRDREITYKEFADALGYPLNEKTLALFQK 280 M DD+ +K+F AVD NKNG IS +EL+++ K D +EK ++ FDT+ ++ I+Y E +G P N+ T A+F+K Sbjct: 1 MDDDVVTKIFKAVDKNKNGEISQDELKEYFKKTKVADCDIEKRITDWMGKFDTDSNKSISYSEMCKVVGIPENQNTKAMFKK 82
BLAST of Calcium-binding protein A vs. Planmine SMEST
Match: SMESG000019835.1 (SMESG000019835.1) HSP 1 Score: 46.2098 bits (108), Expect = 8.504e-7 Identity = 24/70 (34.29%), Postives = 42/70 (60.00%), Query Frame = 2 Query: 68 KMFLAVDINKNGIISIEELRKHCSKCSNVDEAVEKFLKNFDTNRDREITYKEFADALGYPLNEKTLALFQ 277 K F A+D + +G I+++EL+ + K + D V K+L FD+N+ +ITYK + D LG E+ + + + Sbjct: 6 KAFEAIDEDGSGEITVDELQHYMIKNNYKDSFVSKWLTLFDSNQSGKITYKHYCDTLGLVPREREIPIVK 75
BLAST of Calcium-binding protein A vs. Planmine SMEST
Match: SMESG000018598.1 (SMESG000018598.1) HSP 1 Score: 43.1282 bits (100), Expect = 1.515e-6 Identity = 24/69 (34.78%), Postives = 35/69 (50.72%), Query Frame = 2 Query: 74 FLAVDINKNGIISIEELRKHCSKCSNVDEAVEKFLKNFDTNRDREITYKEFADALGYPLNEKTLALFQK 280 FL +D NKNGII+++E D E K F++N ITY EF + G ++ T+ F+K Sbjct: 7 FLKIDSNKNGIITLKEFEDAKKSIGKAD--AEAIRKQFESNSGNNITYAEFCNLFGIAPDDGTMKKFKK 73 The following BLAST results are available for this feature:
BLAST of Calcium-binding protein A vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Calcium-binding protein A vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Calcium-binding protein A vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Calcium-binding protein A vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Calcium-binding protein A vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Calcium-binding protein A vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Calcium-binding protein A vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Calcium-binding protein A vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 4
BLAST of Calcium-binding protein A vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Calcium-binding protein A vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Calcium-binding protein A vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Calcium-binding protein A vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Calcium-binding protein A vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Calcium-binding protein A vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30011700 ID=SMED30011700|Name=Calcium-binding protein A|organism=Schmidtea mediterranea sexual|type=transcript|length=350bpback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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