CBFD_NFYB_HMF domain-containing protein

Overview


Name	CBFD_NFYB_HMF domain-containing protein
Smed ID	SMED30011687
Length (bp)	481

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of CBFD_NFYB_HMF domain-containing protein (SMED30011687) t-SNE clustered cells

Violin plots show distribution of expression levels for CBFD_NFYB_HMF domain-containing protein (SMED30011687) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of CBFD_NFYB_HMF domain-containing protein (SMED30011687) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for CBFD_NFYB_HMF domain-containing protein (SMED30011687) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30011687

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 7

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
X1 cell	SMED30011687	h1SMcG0019528	SmedASXL_000275	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
X2 cell	SMED30011687	h1SMcG0019528	SmedASXL_000275	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
neoblast	SMED30011687	h1SMcG0019528	dd_Smed_v4_12599_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parapharyngeal region	SMED30011687	h1SMcG0019528	dd_Smed_v6_12599_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
zeta neoblast	SMED30011687	h1SMcG0019528	dd_Smed_v4_12599_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
neoblast	SMED30011687	h1SMcG0019528	SMED_07069_V2_1	GPL14150	PMID:22385657 Wagner et al., 2012
neoblast	SMED30011687	h1SMcG0019528	SMED_07069_V2_1	GPL14150_gene_models	PMID:22385657 Wagner et al., 2012

Note: Hover over icons to view figure legend

Homology

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BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Human
Match: DRAP1 (DR1 associated protein 1 [Source:HGNC Symbol;Acc:HGNC:3019])

HSP 1 Score: 56.6102 bits (135), Expect = 8.016e-10
Identity = 28/75 (37.33%), Postives = 43/75 (57.33%), Query Frame = 3

Query:  237 NLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFL 461
            N  FP +RIK I++ + EIG +      +I++A E F+E L+K A    Q  N KT+T +HL + I+     +FL
Sbjct:    9 NARFPPARIKKIMQTDEEIGKVAAAVPVIISRALELFLESLLKKACQVTQSRNAKTMTTSHLKQCIELEQQFDFL 83

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BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Human
Match: DRAP1 (DR1 associated protein 1 [Source:HGNC Symbol;Acc:HGNC:3019])

HSP 1 Score: 56.6102 bits (135), Expect = 8.541e-10
Identity = 28/75 (37.33%), Postives = 43/75 (57.33%), Query Frame = 3

Query:  237 NLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFL 461
            N  FP +RIK I++ + EIG +      +I++A E F+E L+K A    Q  N KT+T +HL + I+     +FL
Sbjct:    9 NARFPPARIKKIMQTDEEIGKVAAAVPVIISRALELFLESLLKKACQVTQSRNAKTMTTSHLKQCIELEQQFDFL 83

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BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Human
Match: POLE4 (DNA polymerase epsilon 4, accessory subunit [Source:HGNC Symbol;Acc:HGNC:18755])

HSP 1 Score: 52.7582 bits (125), Expect = 5.392e-9
Identity = 25/77 (32.47%), Postives = 44/77 (57.14%), Query Frame = 3

Query:  246 FPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFLDGMLD 476
             P +R+KA++K + ++ +   ++I ++ +AAE FVE + K A   AQ   +KT+ +  L   I+      FL+G LD
Sbjct:   41 LPLARVKALVKADPDVTLAGQEAIFILARAAELFVETIAKDAYCCAQQGKRKTLQRRDLDNAIEAVDEFAFLEGTLD 117

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BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Celegans
Match: pole-4 (pep chromosome:WBcel235:II:14968633:14969354:-1 gene:WBGene00013150.1 transcript:Y53F4B.3.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:pole-4)

HSP 1 Score: 52.373 bits (124), Expect = 8.763e-9
Identity = 31/102 (30.39%), Postives = 56/102 (54.90%), Query Frame = 3

Query:  180 IIDASFKDNEELDEHIATKNLF---FPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFLDGMLD 476
            I   S +D  E++EH+  + L     P  R+K ++++N ++ ++NN+++ L+ KAAE F++EL   A   A L  +KT+    + + I       FL+  LD
Sbjct:   13 IAQMSEEDVREIEEHV--EELVRSQLPLGRVKKVVRMNPDVEMLNNEALQLMAKAAELFIKELSNAANQNAALEKRKTVQTKDIDKAIKKTWAFAFLEDALD 112

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BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Fly
Match: Chrac-16 (gene:FBgn0043001 transcript:FBtr0073642)

HSP 1 Score: 52.373 bits (124), Expect = 5.540e-9
Identity = 27/83 (32.53%), Postives = 44/83 (53.01%), Query Frame = 3

Query:  219 EHIATKNLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNK--KTITKNHLIEFIDDNSNMNFL 461
            E   T   F P SR++ I+K + + G+I N+ + L+ K  E FV  L  GAAY  +   +  + +   HL + ++ N N+ FL
Sbjct:   11 ERPPTAETFLPLSRVRTIMKSSMDTGLITNEVLFLMTKCTELFVRHLA-GAAYTEEFGQRPGEALKYEHLSQVVNKNKNLEFL 92

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BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Fly
Match: Chrac-16 (gene:FBgn0043001 transcript:FBtr0339625)

HSP 1 Score: 52.373 bits (124), Expect = 5.540e-9
Identity = 27/83 (32.53%), Postives = 44/83 (53.01%), Query Frame = 3

Query:  219 EHIATKNLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNK--KTITKNHLIEFIDDNSNMNFL 461
            E   T   F P SR++ I+K + + G+I N+ + L+ K  E FV  L  GAAY  +   +  + +   HL + ++ N N+ FL
Sbjct:   11 ERPPTAETFLPLSRVRTIMKSSMDTGLITNEVLFLMTKCTELFVRHLA-GAAYTEEFGQRPGEALKYEHLSQVVNKNKNLEFL 92

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BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Fly
Match: Mes4 (gene:FBgn0034726 transcript:FBtr0071827)

HSP 1 Score: 46.595 bits (109), Expect = 8.338e-7
Identity = 24/77 (31.17%), Postives = 45/77 (58.44%), Query Frame = 3

Query:  246 FPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFLDGMLD 476
             P +RI+ I+KL+ ++ + NN+++ ++ KA E F+  L + +  +   S KKTI K  +   I    ++ FLDG ++
Sbjct:   78 LPLARIRNIMKLDPDLHMANNEAVFIVAKAVELFIASLSRESYTYTAQSKKKTIQKRDVDMAISAVDSLLFLDGAMN 154

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BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Fly
Match: Mes4 (gene:FBgn0034726 transcript:FBtr0301243)

HSP 1 Score: 46.595 bits (109), Expect = 8.338e-7
Identity = 24/77 (31.17%), Postives = 45/77 (58.44%), Query Frame = 3

Query:  246 FPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFLDGMLD 476
             P +RI+ I+KL+ ++ + NN+++ ++ KA E F+  L + +  +   S KKTI K  +   I    ++ FLDG ++
Sbjct:   78 LPLARIRNIMKLDPDLHMANNEAVFIVAKAVELFIASLSRESYTYTAQSKKKTIQKRDVDMAISAVDSLLFLDGAMN 154

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BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Zebrafish
Match: pole4 (polymerase (DNA-directed), epsilon 4, accessory subunit [Source:ZFIN;Acc:ZDB-GENE-050522-309])

HSP 1 Score: 53.5286 bits (127), Expect = 2.170e-9
Identity = 30/80 (37.50%), Postives = 47/80 (58.75%), Query Frame = 3

Query:  246 FPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHL---IEFIDDNSNMNFLDGMLD 476
             P SRIK ++K + ++ + + +S+ +I KA E FVE + K A  +AQ   +KT+ +  L   IE ID+     FL+G LD
Sbjct:   51 LPLSRIKTLMKADPDVTLASQESVFIIAKATELFVEMIAKDALVYAQQGKRKTLQRKDLDNAIEAIDE---FAFLEGTLD 127

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BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Zebrafish
Match: drap1 (DR1-associated protein 1 (negative cofactor 2 alpha) [Source:ZFIN;Acc:ZDB-GENE-030131-400])

HSP 1 Score: 53.1434 bits (126), Expect = 9.115e-9
Identity = 27/75 (36.00%), Postives = 42/75 (56.00%), Query Frame = 3

Query:  237 NLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFL 461
            N  FP +RIK I++ + EIG +      +I++A E F+E L+  A    Q  N KT+T +HL + I+     +FL
Sbjct:    9 NARFPPARIKKIMQTDEEIGKVAAAVPVIISRALELFLESLLTKACDVTQSRNAKTMTTSHLKQCIELEQQFDFL 83

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Zebrafish
Match: pole4 (polymerase (DNA-directed), epsilon 4, accessory subunit [Source:ZFIN;Acc:ZDB-GENE-050522-309])

HSP 1 Score: 48.9062 bits (115), Expect = 2.343e-7
Identity = 28/77 (36.36%), Postives = 45/77 (58.44%), Query Frame = 3

Query:  246 FPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHL---IEFIDDNSNMNFLDG 467
             P SRIK ++K + ++ + + +S+ +I KA E FVE + K A  +AQ   +KT+ +  L   IE ID+     FL+G
Sbjct:   51 LPLSRIKTLMKADPDVTLASQESVFIIAKATELFVEMIAKDALVYAQQGKRKTLQRKDLDNAIEAIDE---FAFLEG 124

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Zebrafish
Match: pole4 (polymerase (DNA-directed), epsilon 4, accessory subunit [Source:ZFIN;Acc:ZDB-GENE-050522-309])

HSP 1 Score: 46.595 bits (109), Expect = 5.482e-7
Identity = 21/59 (35.59%), Postives = 36/59 (61.02%), Query Frame = 3

Query:  246 FPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHL 422
             P SRIK ++K + ++ + + +S+ +I KA E FVE + K A  +AQ   +KT+ +  L
Sbjct:   51 LPLSRIKTLMKADPDVTLASQESVFIIAKATELFVEMIAKDALVYAQQGKRKTLQRKDL 109

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Zebrafish
Match: pole4 (polymerase (DNA-directed), epsilon 4, accessory subunit [Source:ZFIN;Acc:ZDB-GENE-050522-309])

HSP 1 Score: 46.595 bits (109), Expect = 6.146e-7
Identity = 21/59 (35.59%), Postives = 36/59 (61.02%), Query Frame = 3

Query:  246 FPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHL 422
             P SRIK ++K + ++ + + +S+ +I KA E FVE + K A  +AQ   +KT+ +  L
Sbjct:   51 LPLSRIKTLMKADPDVTLASQESVFIIAKATELFVEMIAKDALVYAQQGKRKTLQRKDL 109

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BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Xenopus
Match: pole4 (polymerase (DNA-directed), epsilon 4, accessory subunit [Source:NCBI gene;Acc:549359])

HSP 1 Score: 57.7658 bits (138), Expect = 5.640e-11
Identity = 29/81 (35.80%), Postives = 47/81 (58.02%), Query Frame = 3

Query:  234 KNLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFLDGMLD 476
            K    P SRIKA++K + ++ + + +S+ +I+KA E F+E + K A  +AQ   +KT+ +  L   ID      FL+G LD
Sbjct:   33 KQARLPLSRIKALMKADPDLSLASQESVFVISKATELFIETIAKDAYLYAQQGKRKTLQRKDLDNAIDAIDEFAFLEGTLD 113

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BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Xenopus
Match: pole4 (polymerase (DNA-directed), epsilon 4, accessory subunit [Source:NCBI gene;Acc:549359])

HSP 1 Score: 50.8322 bits (120), Expect = 3.123e-8
Identity = 23/70 (32.86%), Postives = 43/70 (61.43%), Query Frame = 3

Query:  234 KNLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDN 443
            K    P SRIKA++K + ++ + + +S+ +I+KA E F+E + K A  +AQ   +KT+ +  L  +I ++
Sbjct:   33 KQARLPLSRIKALMKADPDLSLASQESVFVISKATELFIETIAKDAYLYAQQGKRKTLQRKDLGMYIAED 102

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BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Xenopus
Match: arl14 (ADP ribosylation factor like GTPase 14 [Source:Xenbase;Acc:XB-GENE-5750500])

HSP 1 Score: 48.1358 bits (113), Expect = 8.789e-7
Identity = 24/69 (34.78%), Postives = 40/69 (57.97%), Query Frame = 3

Query:  255 SRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFL 461
            +RIK I++ + EIG +      +I++A E F+E L+K A    Q  + KT+T +HL + I+     +FL
Sbjct:    1 ARIKKIMQTDEEIGKVAAAVPVIISRALELFLESLLKKACQVTQSRSAKTMTTSHLKQCIELEQQFDFL 69

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BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Mouse
Match: Drap1 (Dr1 associated protein 1 (negative cofactor 2 alpha) [Source:MGI Symbol;Acc:MGI:1913806])

HSP 1 Score: 56.6102 bits (135), Expect = 4.185e-10
Identity = 28/75 (37.33%), Postives = 43/75 (57.33%), Query Frame = 3

Query:  237 NLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFL 461
            N  FP +RIK I++ + EIG +      +I++A E F+E L+K A    Q  N KT+T +HL + I+     +FL
Sbjct:    9 NARFPPARIKKIMQTDEEIGKVAAAVPVIISRALELFLESLLKKACQVTQSRNAKTMTTSHLKQCIELEQQFDFL 83

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Mouse
Match: Drap1 (Dr1 associated protein 1 (negative cofactor 2 alpha) [Source:MGI Symbol;Acc:MGI:1913806])

HSP 1 Score: 56.6102 bits (135), Expect = 5.297e-10
Identity = 28/75 (37.33%), Postives = 43/75 (57.33%), Query Frame = 3

Query:  237 NLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFL 461
            N  FP +RIK I++ + EIG +      +I++A E F+E L+K A    Q  N KT+T +HL + I+     +FL
Sbjct:    9 NARFPPARIKKIMQTDEEIGKVAAAVPVIISRALELFLESLLKKACQVTQSRNAKTMTTSHLKQCIELEQQFDFL 83

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Mouse
Match: Drap1 (Dr1 associated protein 1 (negative cofactor 2 alpha) [Source:MGI Symbol;Acc:MGI:1913806])

HSP 1 Score: 56.6102 bits (135), Expect = 5.559e-10
Identity = 28/75 (37.33%), Postives = 43/75 (57.33%), Query Frame = 3

Query:  237 NLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFL 461
            N  FP +RIK I++ + EIG +      +I++A E F+E L+K A    Q  N KT+T +HL + I+     +FL
Sbjct:    9 NARFPPARIKKIMQTDEEIGKVAAAVPVIISRALELFLESLLKKACQVTQSRNAKTMTTSHLKQCIELEQQFDFL 83

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Mouse
Match: Pole4 (polymerase (DNA-directed), epsilon 4 (p12 subunit) [Source:MGI Symbol;Acc:MGI:1914229])

HSP 1 Score: 52.7582 bits (125), Expect = 4.066e-9
Identity = 25/77 (32.47%), Postives = 44/77 (57.14%), Query Frame = 3

Query:  246 FPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFLDGMLD 476
             P +R+KA++K + ++ +   ++I ++ +AAE FVE + K A   AQ   +KT+ +  L   I+      FL+G LD
Sbjct:   42 LPLARVKALVKADPDVTLAGQEAIFILARAAELFVETIAKDAYCCAQQGKRKTLQRRDLDNAIEAVDEFAFLEGTLD 118

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Mouse
Match: Pole4 (polymerase (DNA-directed), epsilon 4 (p12 subunit) [Source:MGI Symbol;Acc:MGI:1914229])

HSP 1 Score: 49.6766 bits (117), Expect = 4.616e-8
Identity = 23/75 (30.67%), Postives = 43/75 (57.33%), Query Frame = 3

Query:  246 FPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFLDGM 470
             P +R+KA++K + ++ +   ++I ++ +AAE FVE + K A   AQ   +KT+ +  L   I+      FL+G+
Sbjct:   42 LPLARVKALVKADPDVTLAGQEAIFILARAAELFVETIAKDAYCCAQQGKRKTLQRRDLDNAIEAVDEFAFLEGL 116

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BLAST of CBFD_NFYB_HMF domain-containing protein vs. UniProt/SwissProt
Match: sp|Q2YDP3|NC2A_BOVIN (Dr1-associated corepressor OS=Bos taurus OX=9913 GN=DRAP1 PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 3.668e-9
Identity = 28/75 (37.33%), Postives = 43/75 (57.33%), Query Frame = 3

Query:  237 NLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFL 461
            N  FP +RIK I++ + EIG +      +I++A E F+E L+K A    Q  N KT+T +HL + I+     +FL
Sbjct:    9 NARFPPARIKKIMQTDEEIGKVAAAVPVIISRALELFLESLLKKACQVTQSRNAKTMTTSHLKQCIELEQQFDFL 83

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9D6N5|NC2A_MOUSE (Dr1-associated corepressor OS=Mus musculus OX=10090 GN=Drap1 PE=1 SV=3)

HSP 1 Score: 56.6102 bits (135), Expect = 3.705e-9
Identity = 28/75 (37.33%), Postives = 43/75 (57.33%), Query Frame = 3

Query:  237 NLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFL 461
            N  FP +RIK I++ + EIG +      +I++A E F+E L+K A    Q  N KT+T +HL + I+     +FL
Sbjct:    9 NARFPPARIKKIMQTDEEIGKVAAAVPVIISRALELFLESLLKKACQVTQSRNAKTMTTSHLKQCIELEQQFDFL 83

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. UniProt/SwissProt
Match: sp|A0JPP1|NC2A_RAT (Dr1-associated corepressor OS=Rattus norvegicus OX=10116 GN=Drap1 PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 3.743e-9
Identity = 28/75 (37.33%), Postives = 43/75 (57.33%), Query Frame = 3

Query:  237 NLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFL 461
            N  FP +RIK I++ + EIG +      +I++A E F+E L+K A    Q  N KT+T +HL + I+     +FL
Sbjct:    9 NARFPPARIKKIMQTDEEIGKVAAAVPVIISRALELFLESLLKKACQVTQSRNAKTMTTSHLKQCIELEQQFDFL 83

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. UniProt/SwissProt
Match: sp|Q14919|NC2A_HUMAN (Dr1-associated corepressor OS=Homo sapiens OX=9606 GN=DRAP1 PE=1 SV=3)

HSP 1 Score: 56.6102 bits (135), Expect = 4.101e-9
Identity = 28/75 (37.33%), Postives = 43/75 (57.33%), Query Frame = 3

Query:  237 NLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFL 461
            N  FP +RIK I++ + EIG +      +I++A E F+E L+K A    Q  N KT+T +HL + I+     +FL
Sbjct:    9 NARFPPARIKKIMQTDEEIGKVAAAVPVIISRALELFLESLLKKACQVTQSRNAKTMTTSHLKQCIELEQQFDFL 83

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. UniProt/SwissProt
Match: sp|A6QQ14|DPOE4_BOVIN (DNA polymerase epsilon subunit 4 OS=Bos taurus OX=9913 GN=POLE4 PE=3 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 2.261e-8
Identity = 25/77 (32.47%), Postives = 44/77 (57.14%), Query Frame = 3

Query:  246 FPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFLDGMLD 476
             P +R+KA++K + ++ +   ++I ++ +AAE FVE + K A   AQ   +KT+ +  L   I+      FL+G LD
Sbjct:   40 LPLARVKALVKADPDVTLAGQEAIFILARAAELFVETIAKDAYCCAQQGKRKTLQRRDLDNAIEAVDEFAFLEGTLD 116

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. TrEMBL
Match: E9IEX9 (CBFD_NFYB_HMF domain-containing protein (Fragment) OS=Solenopsis invicta OX=13686 GN=SINV_09843 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 8.315e-12
Identity = 43/120 (35.83%), Postives = 67/120 (55.83%), Query Frame = 3

Query:  120 SFILQFGDSDHDQINSQD-EEIIDASFKDNEELDEHIATKNLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFLDGMLD 476
            S  +Q   S  D+ NS++  E +D    D   +DE    K +  P  RIK IIK++ E+G+IN ++  L+ K+ E+F+E L K A  +   S K+T+ K  +   ID+   + FL+GMLD
Sbjct:   10 SKTIQLDKSQVDEENSRNSREDVD----DTPHVDEEQREKLIRLPLGRIKTIIKMDPEVGLINQEAAFLVAKSVEFFIESLAKEAYKYTVQSKKRTVQKRDVENAIDNVDALVFLEGMLD 125

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. TrEMBL
Match: A0A3P7L4J6 (CBFD_NFYB_HMF domain-containing protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS7415 PE=4 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 5.936e-11
Identity = 37/100 (37.00%), Postives = 57/100 (57.00%), Query Frame = 3

Query:  186 DASFKDNEELDEHIATKN---LFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFLDGMLD 476
            D+  KD   + E   T +   + FP SR+K I+K    + +IN++S+A+++ AAE F+ +L   AA  A  S KKT+++N L   +       FLDGMLD
Sbjct:   88 DSPHKDEGAVPEEAETSSVRLIRFPVSRLKTIVKTVPSVHLINSESLAVLSHAAERFIADLTLEAARMATESGKKTVSRNSLDAVVATLPQYEFLDGMLD 187

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. TrEMBL
Match: A0A5N4ADX6 (Uncharacterized protein OS=Photinus pyralis OX=7054 GN=PPYR_12369 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 9.126e-11
Identity = 31/77 (40.26%), Postives = 48/77 (62.34%), Query Frame = 3

Query:  246 FPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFLDGMLD 476
             P +R+K I+KL+ E G I+ DS+ L+ KAAE F++ L + AA +A  S +KT+ +  +   ID    + FL+G LD
Sbjct:   43 LPLTRVKTIMKLDPENGKISQDSVVLVTKAAELFLQYLGREAAKYAMQSKRKTVQRRDINTVIDSVPQLCFLEGALD 119

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. TrEMBL
Match: A0A1Y1LJ31 (CBFD_NFYB_HMF domain-containing protein OS=Photinus pyralis OX=7054 PE=4 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 9.938e-11
Identity = 31/77 (40.26%), Postives = 48/77 (62.34%), Query Frame = 3

Query:  246 FPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFLDGMLD 476
             P +R+K I+KL+ E G I+ DS+ L+ KAAE F++ L + AA +A  S +KT+ +  +   ID    + FL+G LD
Sbjct:   43 LPLTRVKTIMKLDPENGKISQDSVVLVTKAAELFLQYLGREAAKYAMQSKRKTVQRRDINTVIDSVPQLCFLEGALD 119

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. TrEMBL
Match: U6IUL5 (DNA polymerase epsilon subunit OS=Echinococcus granulosus OX=6210 GN=EGR_00312 PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.099e-10
Identity = 39/113 (34.51%), Postives = 59/113 (52.21%), Query Frame = 3

Query:  135 FGDSDHDQINSQDEEIIDASFKDNEELDEHIATKNLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFLDGML 473
             G+SD D     D ++         E  E + +K L  P SRIK+IIK    + ++N++++ALI K+ E+F+ EL + A      S KKT++  H+   I       FLDGML
Sbjct:   48 IGESDRDNDEVSDAQVESGGVATLSEPAE-VPSKLLHLPTSRIKSIIKTVPAVHLVNSEAVALIGKSTEFFLNELAREAHQMIVESGKKTLSMVHIEGVIQTLPQFEFLDGML 159

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Cavefish
Match: pole4 (DNA polymerase epsilon 4, accessory subunit [Source:NCBI gene;Acc:103045108])

HSP 1 Score: 54.6842 bits (130), Expect = 9.606e-10
Identity = 31/80 (38.75%), Postives = 48/80 (60.00%), Query Frame = 3

Query:  246 FPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHL---IEFIDDNSNMNFLDGMLD 476
             P SRIKA++K + ++ + + +S+ +I KA E FVE + K A  +AQ   +KT+ +  L   IE ID+     FL+G LD
Sbjct:   64 LPLSRIKALMKADPDLSLASQESVFIIAKATELFVEMIAKDALVYAQQGKRKTLQRKDLDNAIEAIDE---FAFLEGTLD 140

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Cavefish
Match: drap1 (DR1 associated protein 1 [Source:NCBI gene;Acc:103031620])

HSP 1 Score: 55.4546 bits (132), Expect = 1.329e-9
Identity = 27/75 (36.00%), Postives = 43/75 (57.33%), Query Frame = 3

Query:  237 NLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFL 461
            N  FP +RIK I++ + EIG +      +I++A E F+E L+  A +  Q  N KT+T +HL + I+     +FL
Sbjct:    9 NARFPPARIKKIMQTDEEIGKVAAAVPVIISRALELFLESLLTKACHVTQSRNAKTMTTSHLKQCIELEQQFDFL 83

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Cavefish
Match: pole4 (DNA polymerase epsilon 4, accessory subunit [Source:NCBI gene;Acc:103045108])

HSP 1 Score: 50.8322 bits (120), Expect = 5.264e-8
Identity = 29/78 (37.18%), Postives = 47/78 (60.26%), Query Frame = 3

Query:  246 FPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHL---IEFIDDNSNMNFLDGM 470
             P SRIKA++K + ++ + + +S+ +I KA E FVE + K A  +AQ   +KT+ +  L   IE ID+     FL+G+
Sbjct:   64 LPLSRIKALMKADPDLSLASQESVFIIAKATELFVEMIAKDALVYAQQGKRKTLQRKDLDNAIEAIDE---FAFLEGI 138

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Sea Lamprey
Match: drap1 (DR1-associated protein 1 (negative cofactor 2 alpha) [Source:ZFIN;Acc:ZDB-GENE-030131-400])

HSP 1 Score: 54.299 bits (129), Expect = 6.597e-10
Identity = 26/75 (34.67%), Postives = 42/75 (56.00%), Query Frame = 3

Query:  237 NLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFL 461
            N  FP +RIK I++ + E+G +      +I++A E F+E L+  A    Q  N KT+T +HL + I+     +FL
Sbjct:    9 NARFPPARIKKIMQTDEEVGKVAAAVPVIISRALELFLESLLTKACQVTQSRNAKTMTTSHLKQCIEAEQQFDFL 83

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Yeast
Match: DPB3 (Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication [Source:SGD;Acc:S000000482])

HSP 1 Score: 41.9726 bits (97), Expect = 9.940e-6
Identity = 29/84 (34.52%), Postives = 47/84 (55.95%), Query Frame = 3

Query:  225 IATKNLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNK-KT---ITKNHLIEFIDDNSNMNFLD 464
            +  K   FP S++K I K + E  I +N +I+    AAE FV+ LV+ +   AQL++K KT   ++ N + E ++   N  FL+
Sbjct:    5 VKEKAPVFPISKVKKIAKCDPEYVITSNVAISATAFAAELFVQNLVEESLVLAQLNSKGKTSLRLSLNSIEECVEKRDNFRFLE 88

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Nematostella
Match: EDO42541 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S160])

HSP 1 Score: 59.6918 bits (143), Expect = 2.294e-12
Identity = 28/87 (32.18%), Postives = 52/87 (59.77%), Query Frame = 3

Query:  216 DEHIATKNLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFLDGMLD 476
            +E   ++   FP++R++ ++KL+ ++ + N +++ L+ +AAE FVE   K +        +KTI K  L   +DDN  + FL+G+LD
Sbjct:   25 EEEKPSRMTQFPQTRVRNMMKLDPDLQLANKEAVFLVTRAAELFVEYFAKASYKKTIQGKRKTIQKKDLDATVDDNDEVAFLEGVLD 111

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Nematostella
Match: EDO41226 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4W7])

HSP 1 Score: 51.2174 bits (121), Expect = 2.638e-9
Identity = 25/80 (31.25%), Postives = 44/80 (55.00%), Query Frame = 3

Query:  237 NLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFLDGMLD 476
            N  FP +RIK I++ + ++G +      +I+KA E F++ LV+ A  + Q  N KT++  HL   I      +FL  +++
Sbjct:    9 NARFPPARIKKIMQTDEDVGKVAAAVPVIISKALEIFMQTLVEKACNYTQARNAKTLSTAHLKRCITSEQQFDFLKDLVE 88

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Medaka
Match: pole4 (DNA polymerase epsilon 4, accessory subunit [Source:NCBI gene;Acc:101162035])

HSP 1 Score: 54.299 bits (129), Expect = 7.533e-10
Identity = 27/77 (35.06%), Postives = 45/77 (58.44%), Query Frame = 3

Query:  246 FPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFLDGMLD 476
             P +RIKA++K + ++ + + +S+ +I KA E FVE + K A  +AQ   +KT+ +  L   I+      FL+G LD
Sbjct:   48 LPLARIKALMKTDPDVSLASQESVFIIAKATELFVEMIAKDALVYAQQGKRKTLQRKDLDNAIEAVDEFAFLEGTLD 124

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Medaka
Match: drap1 (DR1 associated protein 1 [Source:NCBI gene;Acc:101159386])

HSP 1 Score: 53.1434 bits (126), Expect = 5.710e-9
Identity = 27/75 (36.00%), Postives = 42/75 (56.00%), Query Frame = 3

Query:  237 NLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFL 461
            N  FP +RIK I++ + EIG +      +I++A E F+E L+  A    Q  N KT+T +HL + I+     +FL
Sbjct:    9 NARFPPARIKKIMQTDEEIGKVAAAVPVIISRALELFLESLLTKACQVTQSRNAKTMTMSHLKQCIELEQQFDFL 83

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Medaka
Match: drap1 (DR1 associated protein 1 [Source:NCBI gene;Acc:101159386])

HSP 1 Score: 53.5286 bits (127), Expect = 7.258e-9
Identity = 27/75 (36.00%), Postives = 42/75 (56.00%), Query Frame = 3

Query:  237 NLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFL 461
            N  FP +RIK I++ + EIG +      +I++A E F+E L+  A    Q  N KT+T +HL + I+     +FL
Sbjct:    9 NARFPPARIKKIMQTDEEIGKVAAAVPVIISRALELFLESLLTKACQVTQSRNAKTMTMSHLKQCIELEQQFDFL 83

[Close]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Planmine SMEST
Match: SMESG000070171.1 (SMESG000070171.1)

HSP 1 Score: 285.034 bits (728), Expect = 2.495e-100
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 3

Query:   39 MSDIDIDDVQLSDSDSTASEEIINEIPSFILQFGDSDHDQINSQDEEIIDASFKDNEELDEHIATKNLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFLDGMLD 476
            MSDIDIDDVQLSDSDSTASEEIINEIPSFILQFGDSDHDQINSQDEEIIDASFKDNEELDEHIATKNLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFLDGMLD
Sbjct:    1 MSDIDIDDVQLSDSDSTASEEIINEIPSFILQFGDSDHDQINSQDEEIIDASFKDNEELDEHIATKNLFFPKSRIKAIIKLNNEIGIINNDSIALINKAAEYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFLDGMLD 146

The following BLAST results are available for this feature:

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Human
BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Celegans
BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Fly
BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Zebrafish
BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Xenopus
BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Mouse
BLAST of CBFD_NFYB_HMF domain-containing protein vs. UniProt/SwissProt
BLAST of CBFD_NFYB_HMF domain-containing protein vs. TrEMBL
BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Cavefish
BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Sea Lamprey
BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Yeast
BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Nematostella
BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Medaka
BLAST of CBFD_NFYB_HMF domain-containing protein vs. Planmine SMEST

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3


Match Name	E-value	Identity	Description
DRAP1	8.016e-10	37.33	DR1 associated protein 1 [Source:HGNC Symbol;Acc:H...	[more]
DRAP1	8.541e-10	37.33	DR1 associated protein 1 [Source:HGNC Symbol;Acc:H...	[more]
POLE4	5.392e-9	32.47	DNA polymerase epsilon 4, accessory subunit [Sourc...	[more]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
pole-4	8.763e-9	30.39	pep chromosome:WBcel235:II:14968633:14969354:-1 ge...	[more]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 4


Match Name	E-value	Identity	Description
Chrac-16	5.540e-9	32.53	gene:FBgn0043001 transcript:FBtr0073642	[more]
Chrac-16	5.540e-9	32.53	gene:FBgn0043001 transcript:FBtr0339625	[more]
Mes4	8.338e-7	31.17	gene:FBgn0034726 transcript:FBtr0071827	[more]
Mes4	8.338e-7	31.17	gene:FBgn0034726 transcript:FBtr0301243	[more]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
pole4	2.170e-9	37.50	polymerase (DNA-directed), epsilon 4, accessory su...	[more]
drap1	9.115e-9	36.00	DR1-associated protein 1 (negative cofactor 2 alph...	[more]
pole4	2.343e-7	36.36	polymerase (DNA-directed), epsilon 4, accessory su...	[more]
pole4	5.482e-7	35.59	polymerase (DNA-directed), epsilon 4, accessory su...	[more]
pole4	6.146e-7	35.59	polymerase (DNA-directed), epsilon 4, accessory su...	[more]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3


Match Name	E-value	Identity	Description
pole4	5.640e-11	35.80	polymerase (DNA-directed), epsilon 4, accessory su...	[more]
pole4	3.123e-8	32.86	polymerase (DNA-directed), epsilon 4, accessory su...	[more]
arl14	8.789e-7	34.78	ADP ribosylation factor like GTPase 14 [Source:Xen...	[more]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Drap1	4.185e-10	37.33	Dr1 associated protein 1 (negative cofactor 2 alph...	[more]
Drap1	5.297e-10	37.33	Dr1 associated protein 1 (negative cofactor 2 alph...	[more]
Drap1	5.559e-10	37.33	Dr1 associated protein 1 (negative cofactor 2 alph...	[more]
Pole4	4.066e-9	32.47	polymerase (DNA-directed), epsilon 4 (p12 subunit)...	[more]
Pole4	4.616e-8	30.67	polymerase (DNA-directed), epsilon 4 (p12 subunit)...	[more]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q2YDP3\|NC2A_BOVIN	3.668e-9	37.33	Dr1-associated corepressor OS=Bos taurus OX=9913 G...	[more]
sp\|Q9D6N5\|NC2A_MOUSE	3.705e-9	37.33	Dr1-associated corepressor OS=Mus musculus OX=1009...	[more]
sp\|A0JPP1\|NC2A_RAT	3.743e-9	37.33	Dr1-associated corepressor OS=Rattus norvegicus OX...	[more]
sp\|Q14919\|NC2A_HUMAN	4.101e-9	37.33	Dr1-associated corepressor OS=Homo sapiens OX=9606...	[more]
sp\|A6QQ14\|DPOE4_BOVIN	2.261e-8	32.47	DNA polymerase epsilon subunit 4 OS=Bos taurus OX=...	[more]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
E9IEX9	8.315e-12	35.83	CBFD_NFYB_HMF domain-containing protein (Fragment)...	[more]
A0A3P7L4J6	5.936e-11	37.00	CBFD_NFYB_HMF domain-containing protein OS=Dibothr...	[more]
A0A5N4ADX6	9.126e-11	40.26	Uncharacterized protein OS=Photinus pyralis OX=705...	[more]
A0A1Y1LJ31	9.938e-11	40.26	CBFD_NFYB_HMF domain-containing protein OS=Photinu...	[more]
U6IUL5	1.099e-10	34.51	DNA polymerase epsilon subunit OS=Echinococcus gra...	[more]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
pole4	9.606e-10	38.75	DNA polymerase epsilon 4, accessory subunit [Sourc...	[more]
drap1	1.329e-9	36.00	DR1 associated protein 1 [Source:NCBI gene;Acc:103...	[more]
pole4	5.264e-8	37.18	DNA polymerase epsilon 4, accessory subunit [Sourc...	[more]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
drap1	6.597e-10	34.67	DR1-associated protein 1 (negative cofactor 2 alph...	[more]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
DPB3	9.940e-6	34.52	Third-largest subunit of DNA polymerase II (DNA po...	[more]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2


Match Name	E-value	Identity	Description
EDO42541	2.294e-12	32.18	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO41226	2.638e-9	31.25	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3


Match Name	E-value	Identity	Description
pole4	7.533e-10	35.06	DNA polymerase epsilon 4, accessory subunit [Sourc...	[more]
drap1	5.710e-9	36.00	DR1 associated protein 1 [Source:NCBI gene;Acc:101...	[more]
drap1	7.258e-9	36.00	DR1 associated protein 1 [Source:NCBI gene;Acc:101...	[more]

BLAST of CBFD_NFYB_HMF domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000070171.1	2.495e-100	100.00	SMESG000070171.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30011687 ID=SMED30011687|Name=CBFD_NFYB_HMF domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=481bp
TTTAATTTTAAATTCTAAGTAAAATTAGTTTTCAAAATATGAGTGACATT
GATATCGATGACGTCCAGTTAAGTGATAGTGATTCTACAGCATCTGAAGA
AATCATAAATGAAATTCCCTCATTTATTTTACAGTTTGGAGATTCAGATC
ATGATCAAATAAATTCACAAGATGAAGAAATTATTGATGCTTCTTTCAAG
GATAATGAAGAACTGGATGAACATATCGCAACTAAGAATTTATTTTTTCC
CAAATCTCGAATCAAGGCTATTATTAAGCTTAATAATGAAATTGGAATTA
TTAATAATGATTCCATAGCTTTGATAAATAAAGCTGCTGAATATTTTGTC
GAGGAACTTGTAAAAGGTGCTGCTTATTTCGCTCAGTTATCAAATAAAAA
AACAATTACAAAAAATCACTTGATTGAGTTTATAGATGATAATTCGAATA
TGAATTTTTTAGATGGAATGCTTGATTAGTC

protein sequence of SMED30011687-orf-1

>SMED30011687-orf-1 ID=SMED30011687-orf-1|Name=SMED30011687-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=147bp
MSDIDIDDVQLSDSDSTASEEIINEIPSFILQFGDSDHDQINSQDEEIID
ASFKDNEELDEHIATKNLFFPKSRIKAIIKLNNEIGIINNDSIALINKAA
EYFVEELVKGAAYFAQLSNKKTITKNHLIEFIDDNSNMNFLDGMLD*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000014	zeta neoblast
PLANA:0000420	parapharyngeal region
PLANA:0000429	neoblast
PLANA:0002109	X1 cell
PLANA:0002111	X2 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR009072	Histone-fold
IPR003958	CBFA_NFYB_domain

Vocabulary: **molecular function**
Term	Definition
GO:0046982	protein heterodimerization activity

Vocabulary: **cellular component**
Term	Definition
GO:0005634	nucleus

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003958	Transcription factor CBF/NF-Y/archaeal histone domain	PFAM	PF00808	CBFD_NFYB_HMF	coord: 69..129 e-value: 1.6E-11 score: 44.3
IPR009072	Histone-fold	GENE3D	G3DSA:1.10.20.10		coord: 31..146 e-value: 1.7E-19 score: 72.1
IPR009072	Histone-fold	SUPERFAMILY	SSF47113	Histone-fold	coord: 55..144
None	No IPR available	PANTHER	PTHR10252:SF79	DNA POLYMERASE EPSILON SUBUNIT 4	coord: 64..146
None	No IPR available	PANTHER	PTHR10252	HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED	coord: 64..146