SMED30011554
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30011554 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 52Note: Hover over icons to view figure legend
Homology
BLAST of SMED30011554 vs. Ensembl Celegans
Match: K09F6.5 (pep chromosome:WBcel235:II:2272062:2273384:-1 gene:WBGene00019588.1 transcript:K09F6.5.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:K09F6.5) HSP 1 Score: 56.9954 bits (136), Expect = 3.511e-8 Identity = 40/150 (26.67%), Postives = 70/150 (46.67%), Query Frame = 1
Query: 1732 EFYINGSQTEYLDLNDTLLTVQIKVVDKDRKPLGEPSDVI-------PNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGYGSDDD-----RKKWIK--GSKLFTMCSSLQLDFMDQPKYLLPGVNVHI 2139
EF + S YL+L T L + D++ + P+ + P N + H++ K+ L N+ +S Y +KA E + Y + KN+ LT GY +D+ K ++ G+ + +++ LD M+QP+ LL NV +
Sbjct: 50 EFVLGNSSRSYLNLKRTYLVYTFNITDQNGAIVNIPNPITKDSLVYAPINNIAHSIVKNFSLHINSRLAYHNSSNYAYKAYFENALMYGKEIKNSTLTAAGYFHEDEIGSPTSKGFLNRCGTGTTQVAANISLDLMNQPRVLLNSCNVKL 199
BLAST of SMED30011554 vs. Ensembl Celegans
Match: F54H12.2 (pep chromosome:WBcel235:III:7964581:7965840:1 gene:WBGene00018842.1 transcript:F54H12.2.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F54H12.2) HSP 1 Score: 55.4546 bits (132), Expect = 8.844e-8 Identity = 39/150 (26.00%), Postives = 70/150 (46.67%), Query Frame = 1
Query: 1732 EFYINGSQTEYLDLNDTLLTVQIKVVDKDRKPLGEPSDVI-------PNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGYGSDDD-----RKKWIK--GSKLFTMCSSLQLDFMDQPKYLLPGVNVHI 2139
EF + + YL+L T L + D++ + P+ + P N + H++ K+ L N+ +S Y +KA E + Y + KN+ LT GY +D+ K ++ G+ + +++ LD M+QP+ LL NV +
Sbjct: 29 EFVLGNNSRSYLNLKRTYLVYTFNITDQNGAIVNIPNPITKDSLVYAPINNIAHSIVKNFSLHINSQLAYHNSSNYAYKAYFENALMYGKEIKNSTLTAAGYFHEDEIGTPTSKGFLNRCGTGTTQVAANISLDLMNQPRVLLNSCNVKL 178
BLAST of SMED30011554 vs. Ensembl Celegans
Match: Y57G11C.20 (pep chromosome:WBcel235:IV:14843362:14845311:1 gene:WBGene00013315.1 transcript:Y57G11C.20.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y57G11C.20) HSP 1 Score: 50.447 bits (119), Expect = 5.344e-6 Identity = 46/136 (33.82%), Postives = 72/136 (52.94%), Query Frame = 1
Query: 850 KDGICKFS-LNNNICEVTFDKNLANTLGLDKQTYRASAFGVRKPKIIKLHRQMFIYTNIIDPIIVGDSLVPLLKSVWVDKHDQDEVVNINIDNPMYLPVSTSCINNIEINIRDDSGKFIEFSKDTKTHLTLHFRKL 1254
KDG L++NI F+K+ A LG R S+ K +++Y +I+DPIIVGDS LL SV K E+++ I +P YLP+ S I++I+I + + + I F+ + T + LHF+K+
Sbjct: 516 KDGRVSIDFLDSNILFEEFEKSCAYFLGFHDTIVRESSLAPSKIDYFGNVSTIYLYCDIVDPIIVGDSKNSLL-SVIPCKGKYGEMIHHTIPHPRYLPIMNSTIDSIKIELLSEFAEPINFNWGS-TVIVLHFKKI 649
BLAST of SMED30011554 vs. UniProt/SwissProt
Match: sp|P34456|YMD2_CAEEL (Uncharacterized protein F54H12.2 OS=Caenorhabditis elegans OX=6239 GN=F54H12.2 PE=4 SV=1) HSP 1 Score: 55.4546 bits (132), Expect = 1.125e-6 Identity = 39/150 (26.00%), Postives = 70/150 (46.67%), Query Frame = 1
Query: 1732 EFYINGSQTEYLDLNDTLLTVQIKVVDKDRKPLGEPSDVI-------PNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGYGSDDD-----RKKWIK--GSKLFTMCSSLQLDFMDQPKYLLPGVNVHI 2139
EF + + YL+L T L + D++ + P+ + P N + H++ K+ L N+ +S Y +KA E + Y + KN+ LT GY +D+ K ++ G+ + +++ LD M+QP+ LL NV +
Sbjct: 29 EFVLGNNSRSYLNLKRTYLVYTFNITDQNGAIVNIPNPITKDSLVYAPINNIAHSIVKNFSLHINSQLAYHNSSNYAYKAYFENALMYGKEIKNSTLTAAGYFHEDEIGTPTSKGFLNRCGTGTTQVAANISLDLMNQPRVLLNSCNVKL 178
BLAST of SMED30011554 vs. TrEMBL
Match: A0A3C1P7N7 (Uncharacterized protein (Fragment) OS=Planktothrix sp. UBA8402 OX=2055759 GN=DCQ63_02400 PE=4 SV=1) HSP 1 Score: 167.933 bits (424), Expect = 8.084e-44 Identity = 87/189 (46.03%), Postives = 120/189 (63.49%), Query Frame = 1
Query: 1612 ICGKSELCLFDRPSPQAVIEMGAFEDVFPMNSIMDSRTDIEFYINGSQTEYLDLNDTLLTVQIKVVDKDRKPLGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGY-GSDDDRKKWIKGSKLFTMCSSLQLDFMDQPKYLLPGVNVHIRLKRSESALSLT 2175
+ GK+EL +FD PQ VIE G FEDV+P + T IEF + S +YLDLNDTLL++Q+KVV D L + +P+++ H+LF D L N+ IEGG+ Y +KA+IE+ +N+NS+TK T L G+ + RK W+ SK+ M L+LDF++QPKYLLPGV V I L ++ S SLT
Sbjct: 1 MSGKTELKIFDDHPPQVVIEAGYFEDVYPKAAT--ESTTIEFEVPSSNVDYLDLNDTLLSLQVKVVKADGTALTDKDKPVPSDYFMHSLFSDVSLALNDTTIEGGSGIYPYKAVIESELNFNSETKQTQLAMAGHEPAVLKRKAWVALSKVLEMIGPLRLDFLNQPKYLLPGVKVRIILTKANSNFSLT 187
BLAST of SMED30011554 vs. TrEMBL
Match: W4ZG02 (Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1) HSP 1 Score: 134.035 bits (336), Expect = 1.246e-29 Identity = 85/230 (36.96%), Postives = 125/230 (54.35%), Query Frame = 1
Query: 1615 CGKSELCLFDRPSPQAVIEMGAFEDVFPMNSIMDSRTDIEFYINGSQTEYLDLNDTLLTVQIKVVDKDRKPLGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGY-----GSDDD---------------RKKWIKGSKLFTMCSSLQLDFMDQPKYLLPGVNVHIRLKRSESALSL-TSVTTAPI-LQLVDAKLLVRRVR 2238
C KSE LF P Q +E G + + FP+ +I D+ T I+F++ GS EY DL+ TL+ +Q+KVV+ D PL E + V P N H+LF D L N+ I +TY ++A +ET++ Y + K + LT + G DD R ++IKGSK M L LD + Q K LL GV++ ++L RS+++ SL +SV A + + A L VRRV+
Sbjct: 11 CSKSETDLFSIPPTQTTVEKGKWVEFFPLTNISDT-TPIQFHLQGSTEEYTDLSQTLIHLQVKVVNGDGTPLAEDAQVGPTNMFLHSLFSDVDLMLNDRLITPSTNTYAYRAALETLLTYGPEAKESQLTSALFYKDTPGHMDDGNPLREDAGNMGLKERHRFIKGSKTVDMVGLLHLDMVFQDKLLLGGVDIKLKLNRSKNSFSLMSSVQNAEYKVIITSASLHVRRVK 239
BLAST of SMED30011554 vs. TrEMBL
Match: W4YCC1 (Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1) HSP 1 Score: 127.487 bits (319), Expect = 1.618e-27 Identity = 80/230 (34.78%), Postives = 125/230 (54.35%), Query Frame = 1
Query: 1615 CGKSELCLFDRPSPQAVIEMGAFEDVFPMNSIMDSRTDIEFYINGSQTEYLDLNDTLLTVQIKVVDKDRKPLGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGY-----GSDDD---------------RKKWIKGSKLFTMCSSLQLDFMDQPKYLLPGVNVHIRLKRSESALSL-TSVTTAPILQLV-DAKLLVRRVR 2238
C KSE+ LF P Q +E G + + FP+ +I D+ I+F++ GS EY DL+ +L+ +Q KVV+ D PL + + V P N H+LF D L N+ I +TY ++A +ET++ Y ++ K + L+ + G DD R ++IKGS + M L LD + Q + LL GV++ ++L RS+++ SL +SV A L+ A L VRRV+
Sbjct: 11 CSKSEIDLFSIPPTQTTVEKGKWVEFFPLTNISDT-APIQFHLQGSTEEYTDLSQSLIHLQAKVVNGDGTPLADDAQVGPTNMFLHSLFSDVDLMLNDRLITPSTNTYAYRAALETLLTYGAEAKESQLSSALFYKDTAGHMDDGNPLRNDDGNMGLKERHRFIKGSNIVDMVGVLHLDMVFQDRLLLGGVDIKLKLNRSKNSFSLMSSVENANYKVLITSASLHVRRVK 239
BLAST of SMED30011554 vs. TrEMBL
Match: A0A4Y2TVH4 (Uncharacterized protein OS=Araneus ventricosus OX=182803 GN=AVEN_94531_1 PE=4 SV=1) HSP 1 Score: 121.709 bits (304), Expect = 5.050e-27 Identity = 75/227 (33.04%), Postives = 114/227 (50.22%), Query Frame = 1
Query: 1612 ICGKSELCLFDRPSPQAVIEMGAFEDVFPMNSIMDSRTDIEFYINGSQTEYLDLNDTLLTVQIKVVDKDRKPLGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGYGSDD-----------------DRKKWIKGSKLFTMCSSLQLDFMDQPKYLLPGVNVHIRLKRSESALSLTSVTTAPI-LQLVDAKLLVRRVR 2238
IC KSEL LF PS QA IE G F + FP+++I D + +EF+I+GS EYLDL D+ + V+ K+ D PL + V P N H+LF + N+ I ++TY ++A +ET++NY D K + L+ + DD R + + S+ M L D Q + +L V + I++ RS+ L S + + L A L VR+V+
Sbjct: 11 ICVKSELDLFSIPSTQAAIEFGKFVEYFPLSNIRDG-SPVEFHISGSGDEYLDLADSYIHVKAKITKSDGAPLPDNEPVAPVNLFLHSLFSQVGVSLNDRIISSASNTYPYRAYLETLLNYGEDAKKSLLSCEAFFKDDKPYQVDPVSEEACESLKKRYQLMANSRTLDMIGQLHCDIFQQNRLMLNLVEMKIKMIRSKPNFCLLSTNNSEYNVVLEHASLFVRKVK 236
BLAST of SMED30011554 vs. TrEMBL
Match: A0A4Y2UAH2 (Uncharacterized protein F54H12.2 OS=Araneus ventricosus OX=182803 GN=F54H12.2_85 PE=4 SV=1) HSP 1 Score: 125.176 bits (313), Expect = 8.559e-27 Identity = 76/227 (33.48%), Postives = 117/227 (51.54%), Query Frame = 1
Query: 1612 ICGKSELCLFDRPSPQAVIEMGAFEDVFPMNSIMDSRTDIEFYINGSQTEYLDLNDTLLTVQIKVVDKDRKPLGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGYGSDDD-----------------RKKWIKGSKLFTMCSSLQLDFMDQPKYLLPGVNVHIRLKRSESALSLTSVTTAPI-LQLVDAKLLVRRVR 2238
IC KSEL LF PS QA IE G F + FP+++I D + +EF+I+GS EYLDL D+ + V+ K+ DR PL + V P N H+LF + N+ I ++TY ++A +ET++NY D K + L+ + DD+ R + + S+ M L D Q + +L V++ I++ RS+ L S + + L A L VR+V+
Sbjct: 11 ICVKSELDLFSIPSTQAAIEFGKFVEYFPLSNIRDG-SPVEFHISGSGDEYLDLADSYIHVKAKITKSDRAPLPDNEPVAPVNLFLHSLFSRVDVSLNDRIISSASNTYPYRAYLETLLNYGEDAKKSLLSCEAFFKDDNPYQVDPVSEEACESLKKRYQLMANSRTLDMIGQLHCDIFQQNRLMLNLVDMKIKMIRSKPNFCLLSTNNSEYNVVLEHASLFVRKVK 236
BLAST of SMED30011554 vs. Ensembl Nematostella
Match: EDO39053 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SB46]) HSP 1 Score: 55.0694 bits (131), Expect = 8.076e-8 Identity = 52/230 (22.61%), Postives = 89/230 (38.70%), Query Frame = 1
Query: 1612 ICGKSELCLFDRPSPQAVIEMGAFEDVFPMNSIMDSRTDIEFYINGSQTEYLDLNDTLLTVQIKVVDKDRKPLGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCL--------TPMGYGSDD------------DRKKWIKGSKLFTMCSSLQLDFMDQPKYLLPGVNVHIRLKRSESALSLTSVTTAPILQLVDAKLLVRRVRS 2241
+C + LF P+ + MG + P +I +R I FYI +Y+ L T L +++ D LG V NF L K L N + T ++ + + +Y ++T L TP S D +R + F + L + +YL GV++ ++L + A L S I+++VDA+L + V++
Sbjct: 9 VCANQGIDLFSVPNTDRTLAMGKWNHYAPAETI--TRDKILFYIPAGG-DYIALEKTQLHFTVQIRKPDGTTLGADQKVGFVNFPLAALIKQVTLHLNGTLVCSQTDTSAYRGYVGALTSYTQQAQDTALSAGLWARDTPGAMESYDVMTATGPNRGFVERATYTPLWVNFGLHGVLHTELFQSDRYLPDGVSIKLKLDLNPDAFVLMSENQNEIVRIVDAQLSLFHVKA 235
BLAST of SMED30011554 vs. Planmine SMEST
Match: SMESG000017989.1 (SMESG000017989.1) HSP 1 Score: 692.96 bits (1787), Expect = 0.000e+0 Identity = 335/351 (95.44%), Postives = 342/351 (97.44%), Query Frame = 1
Query: 196 MVWISKPIMDIRKRSNKINFYIILPSNTCPTIHPDNQASKFMVDLQNPIYLHGNWEVALQDFTFVYNTFPFYSHCKIGFKKRVPKTCSGIFSINNFERYCHIPNQILDENCGMFFKDGLNVSCDYSPFTIKFESIEDAKRMGCDKLSIVVPGTEYLIRFKTFDEKSVTIENIKISVSYINEVEDTFTFTNDLYIETLEELHKYISFNCLHIFKTIEFVKDGICKFSLNNNICEVTFDKNLANTLGLDKQTYRASAFGVRKPKIIKLHRQMFIYTNIIDPIIVGDSLVPLLKSVWVDKHDQDEVVNINIDNPMYLPVSTSCINNIEINIRDDSGKFIEFSKDTKTHLTLHFR 1248
M WISK +MDI KR +K++FY ILPSN+CP IHP+NQASKFMVDLQNPIYLHGNWEVALQDFTFVYNTFPFYSHCKI +KKRVPKTCSGIFSINNFERYCHIPNQILDENCGMFFKDGLNVSCDYSPFTIKFESIEDAKRMGCDKLSIVVPGTEYLIRFKTFDEKSVTIENIKISVSYINEVEDTFTFTNDLYIETLEELHKYISFNCLHIFKTIEFVKDGICKFSLNNNICEVTFDKNLANTLGLDKQTYRASAFGVRKPKIIKLHRQMFIYTNIIDPIIVGDSLVPLLKSVWVD HDQDEVVNINIDNPMYLPVSTSCINNIEINIRDDSGKFIEFSKDTKTHLTLHF
Sbjct: 1 MAWISKTLMDIIKRPSKMSFYTILPSNSCPLIHPENQASKFMVDLQNPIYLHGNWEVALQDFTFVYNTFPFYSHCKISYKKRVPKTCSGIFSINNFERYCHIPNQILDENCGMFFKDGLNVSCDYSPFTIKFESIEDAKRMGCDKLSIVVPGTEYLIRFKTFDEKSVTIENIKISVSYINEVEDTFTFTNDLYIETLEELHKYISFNCLHIFKTIEFVKDGICKFSLNNNICEVTFDKNLANTLGLDKQTYRASAFGVRKPKIIKLHRQMFIYTNIIDPIIVGDSLVPLLKSVWVDTHDQDEVVNINIDNPMYLPVSTSCINNIEINIRDDSGKFIEFSKDTKTHLTLHFH 351 HSP 2 Score: 297.745 bits (761), Expect = 1.593e-85 Identity = 159/179 (88.83%), Postives = 160/179 (89.39%), Query Frame = 1
Query: 1756 TEYLDLNDTLLTVQIKVVDKDRKPLGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGYGSDDDRKKWIKGSKLFTMCSSLQLDFMDQPKYLLPGVNVHIRLKRSESALSLTSVTTAPILQLVDAKLLVRRVRSLFQ*P*KCLMSHGLCLN 2292
T + LNDTLLTVQIKVVDKDRKPLGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGYGSDDDRKKWIKGSKLFTMCSSLQLDFMDQPKYLLPGVNVHIRLKRSES LSLTS TT PILQLVDAKLLVRRVR L H L LN
Sbjct: 347 TLHFHLNDTLLTVQIKVVDKDRKPLGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGYGSDDDRKKWIKGSKLFTMCSSLQLDFMDQPKYLLPGVNVHIRLKRSESTLSLTSATTTPILQLVDAKLLVRRVRVELS----VLAGHQLGLN 521
BLAST of SMED30011554 vs. Planmine SMEST
Match: SMESG000048922.1 (SMESG000048922.1) HSP 1 Score: 692.189 bits (1785), Expect = 0.000e+0 Identity = 331/353 (93.77%), Postives = 344/353 (97.45%), Query Frame = 1
Query: 196 MVWISKPIMDIRKRSNKINFYIILPSNTCPTIHPDNQASKFMVDLQNPIYLHGNWEVALQDFTFVYNTFPFYSHCKIGFKKRVPKTCSGIFSINNFERYCHIPNQILDENCGMFFKDGLNVSCDYSPFTIKFESIEDAKRMGCDKLSIVVPGTEYLIRFKTFDEKSVTIENIKISVSYINEVEDTFTFTNDLYIETLEELHKYISFNCLHIFKTIEFVKDGICKFSLNNNICEVTFDKNLANTLGLDKQTYRASAFGVRKPKIIKLHRQMFIYTNIIDPIIVGDSLVPLLKSVWVDKHDQDEVVNINIDNPMYLPVSTSCINNIEINIRDDSGKFIEFSKDTKTHLTLHFRKL 1254
M WISK +MDI KR +K++FY ILPSN+CP IHP+NQASKFMVDLQNPIYLHGNWEVALQDFTFVYNTFPFYSHCKIG+KKRVPKTCSGIFSINNFERYCHIPNQILDENCGMFFKDGLNVSCDYSPFTIKF+SIEDAKRMGCDKLSIVVPGTEYLIRFKTFDEKSVTI+NIKIS+SYINEVE+TFTFTNDLYIETLEELHKYISFNCLHIFKTIEFVKDG+CKFSLNNNICE+TFDKNLANTLGLDKQTYR SAFGV KPKIIKLHRQMFIYTNIIDPIIVGDSLVPLLKSVWVDKHDQDEVVNINIDNPMYLPVSTSCINNIEINIRDDSGKFIEFSKDTKTHLTLHFR L
Sbjct: 1 MAWISKTLMDIIKRPSKMSFYTILPSNSCPLIHPENQASKFMVDLQNPIYLHGNWEVALQDFTFVYNTFPFYSHCKIGYKKRVPKTCSGIFSINNFERYCHIPNQILDENCGMFFKDGLNVSCDYSPFTIKFKSIEDAKRMGCDKLSIVVPGTEYLIRFKTFDEKSVTIKNIKISLSYINEVEETFTFTNDLYIETLEELHKYISFNCLHIFKTIEFVKDGLCKFSLNNNICEITFDKNLANTLGLDKQTYRTSAFGVHKPKIIKLHRQMFIYTNIIDPIIVGDSLVPLLKSVWVDKHDQDEVVNINIDNPMYLPVSTSCINNIEINIRDDSGKFIEFSKDTKTHLTLHFRWL 353 HSP 2 Score: 432.18 bits (1110), Expect = 0.000e+0 Identity = 209/213 (98.12%), Postives = 211/213 (99.06%), Query Frame = 1
Query: 1588 TWNYISKMICGKSELCLFDRPSPQAVIEMGAFEDVFPMNSIMDSRTDIEFYINGSQTEYLDLNDTLLTVQIKVVDKDRKPLGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGYGSDDDRKKWIKGSKLFTMCSSLQLDFMDQPKYLLPGVNVHIRLKRSESALSLTSVTTAPILQLVDAKLLV 2226
TWNYISKMICGKSELCLFDRPSPQAVIEMG FE+VFPMNSIMDSRTDIEFYINGSQTEYLDLNDTLLTVQIKVVDKDRKPLGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGYGSDDDRKKWIKGSKLFTMCSSLQLDFMDQPKYLLPGVNVHIRLKRS+SALSLTS TTAPILQLVDAKLLV
Sbjct: 428 TWNYISKMICGKSELCLFDRPSPQAVIEMGVFEEVFPMNSIMDSRTDIEFYINGSQTEYLDLNDTLLTVQIKVVDKDRKPLGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGYGSDDDRKKWIKGSKLFTMCSSLQLDFMDQPKYLLPGVNVHIRLKRSDSALSLTSATTAPILQLVDAKLLV 640 HSP 3 Score: 125.946 bits (315), Expect = 0.000e+0 Identity = 59/60 (98.33%), Postives = 60/60 (100.00%), Query Frame = 2
Query: 1346 WLRTIGRFALPILKRLGSFGMKTAKDVIMNEQKILPSLKSNALSELGKVLPSVAGMFQKQ 1525
WLRTIGRFALPILKRLGSFGMKT+KDVIMNEQKILPSLKSNALSELGKVLPSVAGMFQKQ
Sbjct: 352 WLRTIGRFALPILKRLGSFGMKTSKDVIMNEQKILPSLKSNALSELGKVLPSVAGMFQKQ 411
BLAST of SMED30011554 vs. Planmine SMEST
Match: SMESG000076652.1 (SMESG000076652.1) HSP 1 Score: 655.596 bits (1690), Expect = 0.000e+0 Identity = 319/358 (89.11%), Postives = 331/358 (92.46%), Query Frame = 1
Query: 196 MVWISKPIMDIRKRSNKINFYIILPSNTCPTIHPDNQASKFMVDLQNPIYLHGNWEVALQDFTFVYNTFPFYSHCKIGFKKRVPKTCSGIFSINNFERYCHIPNQILDENCGMFFKDGLNVSCDYSPFTIKFESIEDAKRMGCDKLSIVVPGTEYLIRFKTFDEKSVTIENIKISVSYINEVEDTFTFTNDLYIETLEELHKYISFNCLHIFKTIEFVKDGICKFSLNNNICEVTFDKNLANTLGLDKQTYRASAFGVRKPKIIKLHRQMFIYTNIIDPIIVGDSLVPLLKSVWVDKHDQDEVVNINIDNPMYLPVSTSCINNIEINIRDDSGKFIEFSKDTKTHLTLHFRKLDGQ*R 1269
M WISK +MDI KR +K++FY ILPSN+CP IHP+NQASKFMVDLQNPIYLHGNWEVALQDFTFVYNTFPFY+HCKI +KKRVPKTCSGIFSINNFERYCHIPNQILDEN GMFFKDGLN DAKRMGCDKLSIVVPGT+YLIRFKTFDEKSVTIENIKISVSYINEVE+TFTFTN+LYIETLEELHKYIS NCLHIFKTIEF+KDGICKFSLNNNICEVTFDKNLANTLGLDKQTYRASAFGVRKPKIIKLHRQMFIYTNIIDPIIVGDSLVPLLKSVWVDKHDQDEVVNINIDNPMYLPVSTSCINNIEINIRDDSGKFIEFSKDTKTHLTLHFRKLDG R
Sbjct: 1 MAWISKTLMDIIKRPSKMSFYTILPSNSCPLIHPENQASKFMVDLQNPIYLHGNWEVALQDFTFVYNTFPFYNHCKISYKKRVPKTCSGIFSINNFERYCHIPNQILDENSGMFFKDGLN----------------DAKRMGCDKLSIVVPGTDYLIRFKTFDEKSVTIENIKISVSYINEVEETFTFTNELYIETLEELHKYISLNCLHIFKTIEFLKDGICKFSLNNNICEVTFDKNLANTLGLDKQTYRASAFGVRKPKIIKLHRQMFIYTNIIDPIIVGDSLVPLLKSVWVDKHDQDEVVNINIDNPMYLPVSTSCINNIEINIRDDSGKFIEFSKDTKTHLTLHFRKLDGWLR 342 HSP 2 Score: 436.417 bits (1121), Expect = 0.000e+0 Identity = 218/237 (91.98%), Postives = 219/237 (92.41%), Query Frame = 1
Query: 1582 ERTWNYISKMICGKSELCLFDRPSPQAVIEMGAFEDVFPMNSIMDSRTDIEFYINGSQTEYLDLNDTLLTVQIKVVDKDRKPLGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGYGSDDDRKKWIKGSKLFTMCSSLQLDFMDQPKYLLPGVNVHIRLKRSESALSLTSVTTAPILQLVDAKLLVRRVRSLFQ*P*KCLMSHGLCLN 2292
ER WNYISKMICGKSELCLFDRPSPQAVIEMGAFEDVFPMNSIMDSRTDIEFYINGSQTEYLDLNDTLLTVQIKVVDKDRK LGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGYGSDDDRKKWIKGSKLFTMCSSLQLDFMDQPKYLL VNVHIRLKRS+SALSLTS TTAPILQLVDAKLLVRRVR L H L LN
Sbjct: 417 ERAWNYISKMICGKSELCLFDRPSPQAVIEMGAFEDVFPMNSIMDSRTDIEFYINGSQTEYLDLNDTLLTVQIKVVDKDRKLLGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGYGSDDDRKKWIKGSKLFTMCSSLQLDFMDQPKYLLLCVNVHIRLKRSDSALSLTSATTAPILQLVDAKLLVRRVRV----EPSVLAGHQLGLN 649 HSP 3 Score: 131.724 bits (330), Expect = 0.000e+0 Identity = 64/67 (95.52%), Postives = 65/67 (97.01%), Query Frame = 2
Query: 1343 GWLRTIGRFALPILKRLGSFGMKTAKDVIMNEQKILPSLKSNALSELGKVLPSVAGMFQKQEPAPAQ 1543
GWLRTIGRFALPILKR+GSFGMKTAKDVIMNEQKILPSLKSNALSELGKVLPSVAGMFQK E APAQ
Sbjct: 339 GWLRTIGRFALPILKRIGSFGMKTAKDVIMNEQKILPSLKSNALSELGKVLPSVAGMFQKPE-APAQ 404
BLAST of SMED30011554 vs. Planmine SMEST
Match: SMESG000048922.1 (SMESG000048922.1) HSP 1 Score: 654.055 bits (1686), Expect = 0.000e+0 Identity = 312/333 (93.69%), Postives = 325/333 (97.60%), Query Frame = 1
Query: 196 MVWISKPIMDIRKRSNKINFYIILPSNTCPTIHPDNQASKFMVDLQNPIYLHGNWEVALQDFTFVYNTFPFYSHCKIGFKKRVPKTCSGIFSINNFERYCHIPNQILDENCGMFFKDGLNVSCDYSPFTIKFESIEDAKRMGCDKLSIVVPGTEYLIRFKTFDEKSVTIENIKISVSYINEVEDTFTFTNDLYIETLEELHKYISFNCLHIFKTIEFVKDGICKFSLNNNICEVTFDKNLANTLGLDKQTYRASAFGVRKPKIIKLHRQMFIYTNIIDPIIVGDSLVPLLKSVWVDKHDQDEVVNINIDNPMYLPVSTSCINNIEINIRDDSG 1194
M WISK +MDI KR +K++FY ILPSN+CP IHP+NQASKFMVDLQNPIYLHGNWEVALQDFTFVYNTFPFYSHCKIG+KKRVPKTCSGIFSINNFERYCHIPNQILDENCGMFFKDGLNVSCDYSPFTIKF+SIEDAKRMGCDKLSIVVPGTEYLIRFKTFDEKSVTI+NIKIS+SYINEVE+TFTFTNDLYIETLEELHKYISFNCLHIFKTIEFVKDG+CKFSLNNNICE+TFDKNLANTLGLDKQTYR SAFGV KPKIIKLHRQMFIYTNIIDPIIVGDSLVPLLKSVWVDKHDQDEVVNINIDNPMYLPVSTSCINNIEINIRDDSG
Sbjct: 1 MAWISKTLMDIIKRPSKMSFYTILPSNSCPLIHPENQASKFMVDLQNPIYLHGNWEVALQDFTFVYNTFPFYSHCKIGYKKRVPKTCSGIFSINNFERYCHIPNQILDENCGMFFKDGLNVSCDYSPFTIKFKSIEDAKRMGCDKLSIVVPGTEYLIRFKTFDEKSVTIKNIKISLSYINEVEETFTFTNDLYIETLEELHKYISFNCLHIFKTIEFVKDGLCKFSLNNNICEITFDKNLANTLGLDKQTYRTSAFGVHKPKIIKLHRQMFIYTNIIDPIIVGDSLVPLLKSVWVDKHDQDEVVNINIDNPMYLPVSTSCINNIEINIRDDSG 333 HSP 2 Score: 431.024 bits (1107), Expect = 1.189e-152 Identity = 209/216 (96.76%), Postives = 213/216 (98.61%), Query Frame = 1
Query: 1579 NERTWNYISKMICGKSELCLFDRPSPQAVIEMGAFEDVFPMNSIMDSRTDIEFYINGSQTEYLDLNDTLLTVQIKVVDKDRKPLGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGYGSDDDRKKWIKGSKLFTMCSSLQLDFMDQPKYLLPGVNVHIRLKRSESALSLTSVTTAPILQLVDAKLLV 2226
++ TWNYISKMICGKSELCLFDRPSPQAVIEMG FE+VFPMNSIMDSRTDIEFYINGSQTEYLDLNDTLLTVQIKVVDKDRKPLGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGYGSDDDRKKWIKGSKLFTMCSSLQLDFMDQPKYLLPGVNVHIRLKRS+SALSLTS TTAPILQLVDAKLLV
Sbjct: 407 SKTTWNYISKMICGKSELCLFDRPSPQAVIEMGVFEEVFPMNSIMDSRTDIEFYINGSQTEYLDLNDTLLTVQIKVVDKDRKPLGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGYGSDDDRKKWIKGSKLFTMCSSLQLDFMDQPKYLLPGVNVHIRLKRSDSALSLTSATTAPILQLVDAKLLV 622 HSP 3 Score: 129.798 bits (325), Expect = 1.189e-152 Identity = 61/62 (98.39%), Postives = 62/62 (100.00%), Query Frame = 2
Query: 1340 SGWLRTIGRFALPILKRLGSFGMKTAKDVIMNEQKILPSLKSNALSELGKVLPSVAGMFQKQ 1525
SGWLRTIGRFALPILKRLGSFGMKT+KDVIMNEQKILPSLKSNALSELGKVLPSVAGMFQKQ
Sbjct: 332 SGWLRTIGRFALPILKRLGSFGMKTSKDVIMNEQKILPSLKSNALSELGKVLPSVAGMFQKQ 393
BLAST of SMED30011554 vs. Planmine SMEST
Match: SMESG000057427.1 (SMESG000057427.1) HSP 1 Score: 634.795 bits (1636), Expect = 0.000e+0 Identity = 306/333 (91.89%), Postives = 319/333 (95.80%), Query Frame = 1
Query: 196 MVWISKPIMDIRKRSNKINFYIILPSNTCPTIHPDNQASKFMVDLQNPIYLHGNWEVALQDFTFVYNTFPFYSHCKIGFKKRVPKTCSGIFSINNFERYCHIPNQILDENCGMFFKDGLNVSCDYSPFTIKFESIEDAKRMGCDKLSIVVPGTEYLIRFKTFDEKSVTIENIKISVSYINEVEDTFTFTNDLYIETLEELHKYISFNCLHIFKTIEFVKDGICKFSLNNNICEVTFDKNLANTLGLDKQTYRASAFGVRKPKIIKLHRQMFIYTNIIDPIIVGDSLVPLLKSVWVDKHDQDEVVNINIDNPMYLPVSTSCINNIEINIRDDSG 1194
M WISK +MDI KR +K++FY ILP N+CP IHP+NQASKFMVDLQNPIYLHGNWEVALQDFTFVYNTFPFYSHCKIG+KKRVPKTCSGIF+INNFERYCHIPNQILDEN GMFFKDGL VSCD+SPFTIKFESIEDAKR+ DKL+IVVPGTEYLIRFK FDEKSVTIENIKISVSYINEVEDTF FTNDLYIETLEELHKYISFNCLHIFKTIEF+KDGICKFSLNNNICEVTFDKNLANTLGLDKQTYRASAFGV KPKIIKLH+QMFIYTNIIDPIIVGDSLVPLLKSVWVDKHDQDEVVNINIDNPMYLPVSTSCINNIEINIRDDSG
Sbjct: 1 MAWISKTLMDIIKRPSKMSFYTILPINSCPLIHPENQASKFMVDLQNPIYLHGNWEVALQDFTFVYNTFPFYSHCKIGYKKRVPKTCSGIFTINNFERYCHIPNQILDENSGMFFKDGLIVSCDFSPFTIKFESIEDAKRIDSDKLNIVVPGTEYLIRFKKFDEKSVTIENIKISVSYINEVEDTFKFTNDLYIETLEELHKYISFNCLHIFKTIEFLKDGICKFSLNNNICEVTFDKNLANTLGLDKQTYRASAFGVLKPKIIKLHKQMFIYTNIIDPIIVGDSLVPLLKSVWVDKHDQDEVVNINIDNPMYLPVSTSCINNIEINIRDDSG 333 HSP 2 Score: 323.553 bits (828), Expect = 7.110e-94 Identity = 158/163 (96.93%), Postives = 160/163 (98.16%), Query Frame = 1
Query: 1735 FYINGSQTEYLDLNDTLLTVQIKVVDKDRKPLGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGYGSDDDRKKWIKGSKLFTMCSSLQLDFMDQPKYLLPGVNVHIRLKRSESALSLTSVTTAPILQLVDAKLL 2223
FYINGSQTEYLDLND LLTVQIKVVDKDRKPLGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGYGSD DRKKWIKGSKLFTMCSSLQLDFMDQPKYLLPGVNVHIRLKRS+SALSLTS +TAPILQLVDAKLL
Sbjct: 407 FYINGSQTEYLDLNDKLLTVQIKVVDKDRKPLGEPSDVIPNNFLFHTLFKDAVLGFNNIKIEGGNSTYIHKALIETIINYNSDTKNTCLTPMGYGSDGDRKKWIKGSKLFTMCSSLQLDFMDQPKYLLPGVNVHIRLKRSDSALSLTSTSTAPILQLVDAKLL 569 HSP 3 Score: 140.584 bits (353), Expect = 1.082e-33 Identity = 66/68 (97.06%), Postives = 67/68 (98.53%), Query Frame = 2
Query: 1340 SGWLRTIGRFALPILKRLGSFGMKTAKDVIMNEQKILPSLKSNALSELGKVLPSVAGMFQKQEPAPAQ 1543
SGWL+TIGRFALPILKRLGSFGMKTAKDVIMNEQKILPSLKSNALSELGKVLPSVA MFQKQEPAPAQ
Sbjct: 332 SGWLKTIGRFALPILKRLGSFGMKTAKDVIMNEQKILPSLKSNALSELGKVLPSVADMFQKQEPAPAQ 399 The following BLAST results are available for this feature:
BLAST of SMED30011554 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30011554 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 3
BLAST of SMED30011554 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30011554 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30011554 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30011554 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30011554 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 1
BLAST of SMED30011554 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30011554 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30011554 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30011554 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30011554 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of SMED30011554 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30011554 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30011554 ID=SMED30011554|Name=SMED30011554|organism=Schmidtea mediterranea sexual|type=transcript|length=2416bpback to top protein sequence of SMED30011554-orf-1 >SMED30011554-orf-1 ID=SMED30011554-orf-1|Name=SMED30011554-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=357bp MVWISKPIMDIRKRSNKINFYIILPSNTCPTIHPDNQASKFMVDLQNPIYback to top protein sequence of SMED30011554-orf-2 >SMED30011554-orf-2 ID=SMED30011554-orf-2|Name=SMED30011554-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=119bp MANNDIISMYSGSQSGGELPYFIGKQYGSGWLRTIGRFALPILKRLGSFGback to top protein sequence of SMED30011554-orf-3 >SMED30011554-orf-3 ID=SMED30011554-orf-3|Name=SMED30011554-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=215bp MICGKSELCLFDRPSPQAVIEMGAFEDVFPMNSIMDSRTDIEFYINGSQTback to top Annotated Terms
The following terms have been associated with this transcript:
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