Centrosomal protein of 19 kDa

Overview
NameCentrosomal protein of 19 kDa
Smed IDSMED30011543
Length (bp)737
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Centrosomal protein of 19 kDa (SMED30011543) t-SNE clustered cells

Violin plots show distribution of expression levels for Centrosomal protein of 19 kDa (SMED30011543) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Centrosomal protein of 19 kDa (SMED30011543) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Centrosomal protein of 19 kDa (SMED30011543) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30011543

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30011543h1SMcG0020530 SmedASXL_019445SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30011543h1SMcG0020530 dd_Smed_v4_2502_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30011543h1SMcG0020530 dd_Smed_v4_2502_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30011543h1SMcG0020530 dd_Smed_v4_2502_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30011543h1SMcG0020530 dd_Smed_v4_2502_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
whole organismSMED30011543h1SMcG0020530 dd_Smed_v4_2502_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult colorimetric in situ hybridization evidence
Category 4 cellSMED30011543h1SMcG0020530 dd_Smed_v4_2502_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
dorsal region of the epidermisSMED30011543h1SMcG0020530 dd_Smed_v4_2502_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Centrosomal protein of 19 kDa vs. Ensembl Human
Match: CEP19 (centrosomal protein 19 [Source:HGNC Symbol;Acc:HGNC:28209])

HSP 1 Score: 83.1889 bits (204), Expect = 3.100e-19
Identity = 54/163 (33.13%), Postives = 91/163 (55.83%), Query Frame = 3 
Query:  141 MNNVINVQKVGIKFHEKIANXXXXXXXXXXXXXXXXXXXXFNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDA-EIIESSKWDSDEGD 626
            M  +   +K GI+F      LI   +   K+++  + +  F++ S       QLK+   H+ YL+     Q+ +L S +R  L G SL +++E+ +    I+P+EDLN +D++ L ++K++MDE F  NQKK  DP+F YDIE  F   + ++S  WD++  D
Sbjct:    3 MGMMCTAKKCGIRFQPPAIILIYESEIKGKIRQRIMPVRNFSKFSDCTRAAEQLKNNPRHKSYLEQVSLRQLEKLFSFLRGYLSGQSLAETMEQIQRETTIDPEEDLNKLDDKELAKRKSIMDELFEKNQKKKDDPNFVYDIEVEFPQDDQLQSCGWDTESAD 165
[Close]
BLAST of Centrosomal protein of 19 kDa vs. Ensembl Human
Match: CEP19 (centrosomal protein 19 [Source:HGNC Symbol;Acc:HGNC:28209])

HSP 1 Score: 80.4925 bits (197), Expect = 1.326e-18
Identity = 44/111 (39.64%), Postives = 70/111 (63.06%), Query Frame = 3 
Query:  297 QLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDA-EIIESSKWDSDEGD 626
            QLK+   H+ YL+     Q+ +L S +R  L G SL +++E+ +    I+P+EDLN +D++ L ++K++MDE F  NQKK  DP+F YDIE  F   + ++S  WD++  D
Sbjct:   16 QLKNNPRHKSYLEQVSLRQLEKLFSFLRGYLSGQSLAETMEQIQRETTIDPEEDLNKLDDKELAKRKSIMDELFEKNQKKKDDPNFVYDIEVEFPQDDQLQSCGWDTESAD 126
[Close]
BLAST of Centrosomal protein of 19 kDa vs. Ensembl Zebrafish
Match: cep19 (centrosomal protein 19 [Source:NCBI gene;Acc:619252])

HSP 1 Score: 62.3882 bits (150), Expect = 1.038e-11
Identity = 44/126 (34.92%), Postives = 71/126 (56.35%), Query Frame = 3 
Query:  261 FNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNF-DAEIIESSKWDSDEGDSGF 635
            F++ S       +LK    H  YL+     Q+ RL  ++RD L+G SL++S+  +R  +    +EDLN + +E L R+KA MD+ F  N+++  DPDF YD+E  F +  + E+  WD ++ D  F
Sbjct:   39 FSQYSDCSRAAERLKHHVRHSVYLECVSLAQLERLHLLLRDHLQGASLEESLAAHRHQE---EEEDLNKLSDEELSRRKAQMDDLFQRNRRRRGDPDFVYDLEVEFGEGSVKETCSWDEEQSDQEF 161
[Close]
BLAST of Centrosomal protein of 19 kDa vs. Ensembl Zebrafish
Match: cep19 (centrosomal protein 19 [Source:NCBI gene;Acc:619252])

HSP 1 Score: 62.3882 bits (150), Expect = 1.038e-11
Identity = 44/126 (34.92%), Postives = 71/126 (56.35%), Query Frame = 3 
Query:  261 FNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNF-DAEIIESSKWDSDEGDSGF 635
            F++ S       +LK    H  YL+     Q+ RL  ++RD L+G SL++S+  +R  +    +EDLN + +E L R+KA MD+ F  N+++  DPDF YD+E  F +  + E+  WD ++ D  F
Sbjct:   39 FSQYSDCSRAAERLKHHVRHSVYLECVSLAQLERLHLLLRDHLQGASLEESLAAHRHQE---EEEDLNKLSDEELSRRKAQMDDLFQRNRRRRGDPDFVYDLEVEFGEGSVKETCSWDEEQSDQEF 161
[Close]
BLAST of Centrosomal protein of 19 kDa vs. Ensembl Zebrafish
Match: cep19 (centrosomal protein 19 [Source:NCBI gene;Acc:619252])

HSP 1 Score: 62.3882 bits (150), Expect = 1.038e-11
Identity = 44/126 (34.92%), Postives = 71/126 (56.35%), Query Frame = 3 
Query:  261 FNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNF-DAEIIESSKWDSDEGDSGF 635
            F++ S       +LK    H  YL+     Q+ RL  ++RD L+G SL++S+  +R  +    +EDLN + +E L R+KA MD+ F  N+++  DPDF YD+E  F +  + E+  WD ++ D  F
Sbjct:   39 FSQYSDCSRAAERLKHHVRHSVYLECVSLAQLERLHLLLRDHLQGASLEESLAAHRHQE---EEEDLNKLSDEELSRRKAQMDDLFQRNRRRRGDPDFVYDLEVEFGEGSVKETCSWDEEQSDQEF 161
[Close]
BLAST of Centrosomal protein of 19 kDa vs. Ensembl Xenopus
Match: cep19 (centrosomal protein 19kDa [Source:NCBI gene;Acc:100135164])

HSP 1 Score: 80.8777 bits (198), Expect = 1.863e-18
Identity = 50/126 (39.68%), Postives = 76/126 (60.32%), Query Frame = 3 
Query:  261 FNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDA-EIIESSKWDSDEGDSGF 635
            F++ S       QLK+   H+ YL      Q+ +L  +++  LKG SL+ ++E+ +  + I+P+EDLN +D++ L ++K VMDE F  N+KK  DPDF+Y+IE +F   E  ES  WD D  D GF
Sbjct:   39 FSKYSDCSRAAEQLKNNPRHKDYLDLVSLRQLEKLYKLLKGHLKGESLKVTLEEIKKEETIDPEEDLNKLDDKELAKRKHVMDELFEKNRKKKDDPDFEYEIEVDFTKDEQAESCGWD-DASDEGF 163
[Close]
BLAST of Centrosomal protein of 19 kDa vs. Ensembl Mouse
Match: Cep19 (centrosomal protein 19 [Source:MGI Symbol;Acc:MGI:1914244])

HSP 1 Score: 77.0258 bits (188), Expect = 4.705e-17
Identity = 45/123 (36.59%), Postives = 71/123 (57.72%), Query Frame = 3 
Query:  261 FNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDA-EIIESSKWDSDEGD 626
            F++ S       QLK+   H+ YL+     Q+ +L   +R  L+G SL +++E+ R    I+P+EDLN +D++ L ++K++MDE F  NQK+  DP F YD+E  F   E + S  WD+   D
Sbjct:   39 FSKFSDCTRAAEQLKNNPRHKSYLEQVPLKQLEKLFVFLRGSLQGQSLAETMEQIRRETTIDPEEDLNKLDDKELAKRKSIMDELFEKNQKRKDDPTFVYDVEVEFPQDEQLLSCSWDTASVD 161
[Close]
BLAST of Centrosomal protein of 19 kDa vs. Ensembl Mouse
Match: Cep19 (centrosomal protein 19 [Source:MGI Symbol;Acc:MGI:1914244])

HSP 1 Score: 77.0258 bits (188), Expect = 4.705e-17
Identity = 45/123 (36.59%), Postives = 71/123 (57.72%), Query Frame = 3 
Query:  261 FNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDA-EIIESSKWDSDEGD 626
            F++ S       QLK+   H+ YL+     Q+ +L   +R  L+G SL +++E+ R    I+P+EDLN +D++ L ++K++MDE F  NQK+  DP F YD+E  F   E + S  WD+   D
Sbjct:   39 FSKFSDCTRAAEQLKNNPRHKSYLEQVPLKQLEKLFVFLRGSLQGQSLAETMEQIRRETTIDPEEDLNKLDDKELAKRKSIMDELFEKNQKRKDDPTFVYDVEVEFPQDEQLLSCSWDTASVD 161
[Close]
BLAST of Centrosomal protein of 19 kDa vs. Ensembl Mouse
Match: Cep19 (centrosomal protein 19 [Source:MGI Symbol;Acc:MGI:1914244])

HSP 1 Score: 77.0258 bits (188), Expect = 4.705e-17
Identity = 45/123 (36.59%), Postives = 71/123 (57.72%), Query Frame = 3 
Query:  261 FNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDA-EIIESSKWDSDEGD 626
            F++ S       QLK+   H+ YL+     Q+ +L   +R  L+G SL +++E+ R    I+P+EDLN +D++ L ++K++MDE F  NQK+  DP F YD+E  F   E + S  WD+   D
Sbjct:   39 FSKFSDCTRAAEQLKNNPRHKSYLEQVPLKQLEKLFVFLRGSLQGQSLAETMEQIRRETTIDPEEDLNKLDDKELAKRKSIMDELFEKNQKRKDDPTFVYDVEVEFPQDEQLLSCSWDTASVD 161
[Close]
BLAST of Centrosomal protein of 19 kDa vs. UniProt/SwissProt
Match: sp|Q96LK0|CEP19_HUMAN (Centrosomal protein of 19 kDa OS=Homo sapiens OX=9606 GN=CEP19 PE=1 SV=2)

HSP 1 Score: 81.2629 bits (199), Expect = 7.523e-18
Identity = 53/160 (33.12%), Postives = 90/160 (56.25%), Query Frame = 3 
Query:  150 VINVQKVGIKFHEKIANXXXXXXXXXXXXXXXXXXXXFNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDA-EIIESSKWDSDEGD 626
            +   +K GI+F      LI   +   K+++  + +  F++ S       QLK+   H+ YL+     Q+ +L S +R  L G SL +++E+ +    I+P+EDLN +D++ L ++K++MDE F  NQKK  DP+F YDIE  F   + ++S  WD++  D
Sbjct:    2 MCTAKKCGIRFQPPAIILIYESEIKGKIRQRIMPVRNFSKFSDCTRAAEQLKNNPRHKSYLEQVSLRQLEKLFSFLRGYLSGQSLAETMEQIQRETTIDPEEDLNKLDDKELAKRKSIMDELFEKNQKKKDDPNFVYDIEVEFPQDDQLQSCGWDTESAD 161
[Close]
BLAST of Centrosomal protein of 19 kDa vs. UniProt/SwissProt
Match: sp|A9UL78|CEP19_XENTR (Centrosomal protein of 19 kDa OS=Xenopus tropicalis OX=8364 GN=cep19 PE=2 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 9.868e-18
Identity = 50/126 (39.68%), Postives = 76/126 (60.32%), Query Frame = 3 
Query:  261 FNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDA-EIIESSKWDSDEGDSGF 635
            F++ S       QLK+   H+ YL      Q+ +L  +++  LKG SL+ ++E+ +  + I+P+EDLN +D++ L ++K VMDE F  N+KK  DPDF+Y+IE +F   E  ES  WD D  D GF
Sbjct:   39 FSKYSDCSRAAEQLKNNPRHKDYLDLVSLRQLEKLYKLLKGHLKGESLKVTLEEIKKEETIDPEEDLNKLDDKELAKRKHVMDELFEKNRKKKDDPDFEYEIEVDFTKDEQAESCGWD-DASDEGF 163
[Close]
BLAST of Centrosomal protein of 19 kDa vs. UniProt/SwissProt
Match: sp|A6H7C9|CEP19_BOVIN (Centrosomal protein of 19 kDa OS=Bos taurus OX=9913 GN=CEP19 PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.826e-17
Identity = 53/160 (33.12%), Postives = 89/160 (55.62%), Query Frame = 3 
Query:  150 VINVQKVGIKFHEKIANXXXXXXXXXXXXXXXXXXXXFNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDA-EIIESSKWDSDEGD 626
            +   +K GI+F      LI   +   K ++  + +  F++ S       QLK+   H+ YL+     Q+ +L S +R  L G SL +++E+ +    I+P+EDLN +D++ L ++K++MDE F  NQKK  DP+F YDIE  F   E ++S  WD++  +
Sbjct:    2 MCTAKKCGIRFQPPAIILIYENEIKGKSRQRIMPVRNFSKYSDCSRAAEQLKNNPRHKGYLEQVSLKQLEKLFSFLRGNLWGQSLAETMEQIQRETTIDPEEDLNKLDDKELAKRKSIMDELFEKNQKKKDDPNFVYDIEVEFPQDEQLQSCGWDTESAE 161
[Close]
BLAST of Centrosomal protein of 19 kDa vs. UniProt/SwissProt
Match: sp|Q9QZX9|CEP19_RAT (Centrosomal protein of 19 kDa OS=Rattus norvegicus OX=10116 GN=Cep19 PE=2 SV=2)

HSP 1 Score: 77.411 bits (189), Expect = 2.128e-16
Identity = 43/119 (36.13%), Postives = 71/119 (59.66%), Query Frame = 3 
Query:  261 FNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDA-EIIESSKWDS 614
            F++ S       QLK+   H+ YL+     Q+ +L   +R  L+G SL +++E+ +    I+P+EDLN +D++ L ++K++MDE F  NQK+  DP F YD+E  F   E ++S  WD+
Sbjct:   39 FSKFSDCTRAAEQLKNNPRHKSYLEQVSLKQLEKLFVFLRGSLQGQSLAETMEQIQRETTIDPEEDLNKLDDKELAKRKSIMDELFEKNQKRKDDPSFVYDVEVEFPQDEQLQSCSWDT 157
[Close]
BLAST of Centrosomal protein of 19 kDa vs. UniProt/SwissProt
Match: sp|Q9CQA8|CEP19_MOUSE (Centrosomal protein of 19 kDa OS=Mus musculus OX=10090 GN=Cep19 PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 3.291e-16
Identity = 45/123 (36.59%), Postives = 71/123 (57.72%), Query Frame = 3 
Query:  261 FNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDA-EIIESSKWDSDEGD 626
            F++ S       QLK+   H+ YL+     Q+ +L   +R  L+G SL +++E+ R    I+P+EDLN +D++ L ++K++MDE F  NQK+  DP F YD+E  F   E + S  WD+   D
Sbjct:   39 FSKFSDCTRAAEQLKNNPRHKSYLEQVPLKQLEKLFVFLRGSLQGQSLAETMEQIRRETTIDPEEDLNKLDDKELAKRKSIMDELFEKNQKRKDDPTFVYDVEVEFPQDEQLLSCSWDTASVD 161
[Close]
BLAST of Centrosomal protein of 19 kDa vs. TrEMBL
Match: A0A433SLU7 (Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_022142 PE=4 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 2.990e-32
Identity = 69/165 (41.82%), Postives = 101/165 (61.21%), Query Frame = 3 
Query:  141 MNNVINVQKVGIKFHEKIANXXXXXXXXXXXXXXXXXXXXFNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDAEIIESSKWDSDEGDSGF 635
            M   + + K G++F      L     +  KL++  + L  FN+N+ V   I+ L S   H  Y++     Q+ RLL+IV+DKL G SL+ SI +N  +D INP+E+LN VD ETL RKK +MD +F   +KKP DPDFQY++E +F+  ++E+S WDS+E D  F
Sbjct:    1 MAETLQLLKCGVRFEPPTLVLSYKDWKSGKLRRRSMPLRNFNKNTGVERIIDDLNSNPRHSRYIRLLSSAQLQRLLTIVKDKLSGLSLEASIARNNAMDTINPEENLNRVDPETLQRKKLIMDTSFEQKRKKPGDPDFQYNVEVDFETAVVETSGWDSEESDVDF 165
[Close]
BLAST of Centrosomal protein of 19 kDa vs. TrEMBL
Match: K1QZR9 (UPF0709 protein C3orf34 OS=Crassostrea gigas OX=29159 GN=CGI_10019881 PE=4 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 8.072e-30
Identity = 58/125 (46.40%), Postives = 88/125 (70.40%), Query Frame = 3 
Query:  261 FNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDAEIIESSKWDSDEGDSGF 635
            FN+NS V   +++L S   H  YLK     Q+ RL+SI++DKL G SL++S+ +N  ID ++P+EDLN +D+ETL RKK++M++TF  N KKP DP F+YD++ +FD   +E+ +WDS+E +  F
Sbjct:    6 FNKNSSVDATVDELLSNSRHSKYLKCMPKFQLYRLVSIIKDKLSGMSLEESLARNDEIDKLDPEEDLNKLDDETLRRKKSIMEDTFEKNLKKPGDPGFEYDVQMDFDE--VEACEWDSEESEQEF 128
[Close]
BLAST of Centrosomal protein of 19 kDa vs. TrEMBL
Match: R7TP07 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_140184 PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 4.775e-28
Identity = 59/125 (47.20%), Postives = 84/125 (67.20%), Query Frame = 3 
Query:  261 FNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDAEIIESSKWDSDEGDSGF 635
            FN+N+ V     +L+  + HE YL +    Q+ +++SI++DKL G  + D ++KN+ ID I+P+EDLN+VDE  L  KKAVMD+TF  N+KKP D DF YD+EK+F+   IES  WDSD   + F
Sbjct:   35 FNKNAGVERVALELQCNKRHEKYLSHVPLHQLKKMISIIKDKLHGLPMDDILKKNKDIDKIDPEEDLNLVDEGILKHKKAVMDQTFERNRKKPGDVDFIYDVEKDFEGGAIESCDWDSDNDSNEF 159
[Close]
BLAST of Centrosomal protein of 19 kDa vs. TrEMBL
Match: A0A2T7ND90 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_21694 PE=4 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 8.393e-27
Identity = 57/123 (46.34%), Postives = 84/123 (68.29%), Query Frame = 3 
Query:  261 FNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDAEI--IESSKWDSDEG 623
            F ++S +   I +L   + H  +++   + Q+ RLL+I++DKL G SL+ SI +N  ID ++P+EDLN VD ETL RKK  MD TF  N+KKP DPDFQY++E +F+ E+  +E+S WDS +G
Sbjct:   42 FTKHSNISSAIKELTENKRHARFVQLLPEAQLHRLLTIMKDKLCGMSLEVSIARNNDIDTLDPNEDLNKVDPETLTRKKLQMDSTFEKNRKKPGDPDFQYNVEIDFETELGRVETSGWDSGDG 164
[Close]
BLAST of Centrosomal protein of 19 kDa vs. TrEMBL
Match: A0A2R2MSZ0 (centrosomal protein of 19 kDa OS=Lingula unguis OX=7574 GN=LOC106161265 PE=4 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 1.844e-25
Identity = 57/155 (36.77%), Postives = 99/155 (63.87%), Query Frame = 3 
Query:  153 INVQKVGIKFHEKIANXXXXXXXXXXXXXXXXXXXXFNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDAEIIESSKWDSD 617
            I ++K GI+F+     +  +     KL +  + +  F +NS +   +++L+  + HE YL+N    Q+ +LL++++DK+KG SL+DS+ +   ++ I+P+EDLN  D+ TL  KKAVM+E+F  N+KKP DP ++YD+E  F+   +E+ +WD D
Sbjct:    4 IQLKKCGIRFNPPAIIVTYLIVSKNKLHRRTMPVRNFTKNSGIQRTVDELRLNKKHEKYLQNIPLRQLEKLLTVIQDKMKGISLEDSLREREKLETIDPEEDLNKADDRTLAAKKAVMEESFEKNRKKPGDPGYEYDVEVEFEGG-VETCEWDDD 157
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BLAST of Centrosomal protein of 19 kDa vs. Ensembl Cavefish
Match: cep19 (centrosomal protein 19 [Source:NCBI gene;Acc:103023470])

HSP 1 Score: 79.337 bits (194), Expect = 4.515e-18
Identity = 45/114 (39.47%), Postives = 65/114 (57.02%), Query Frame = 3 
Query:  297 QLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDA-EIIESSKWDSDEGDSGF 635
            +LK    H  YL++    Q+ RL +++RD L+G +L++S+   RG D    DEDLN + +E L R+KA MD  F  N++   DPDF YD+E  F    + E+  WD D  D  F
Sbjct:   51 RLKHHARHSLYLESVPLAQLERLHTVLRDHLRGLTLEESLAAQRGPD--PNDEDLNKLSDEALNRRKAQMDIVFERNRRHKDDPDFVYDLEVEFPQNSVRETCSWDEDRSDDEF 162
[Close]
BLAST of Centrosomal protein of 19 kDa vs. Ensembl Cavefish
Match: cep19 (centrosomal protein 19 [Source:NCBI gene;Acc:103023470])

HSP 1 Score: 79.337 bits (194), Expect = 4.515e-18
Identity = 45/114 (39.47%), Postives = 65/114 (57.02%), Query Frame = 3 
Query:  297 QLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDA-EIIESSKWDSDEGDSGF 635
            +LK    H  YL++    Q+ RL +++RD L+G +L++S+   RG D    DEDLN + +E L R+KA MD  F  N++   DPDF YD+E  F    + E+  WD D  D  F
Sbjct:   51 RLKHHARHSLYLESVPLAQLERLHTVLRDHLRGLTLEESLAAQRGPD--PNDEDLNKLSDEALNRRKAQMDIVFERNRRHKDDPDFVYDLEVEFPQNSVRETCSWDEDRSDDEF 162
[Close]
BLAST of Centrosomal protein of 19 kDa vs. Ensembl Nematostella
Match: EDO41837 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S374])

HSP 1 Score: 68.5514 bits (166), Expect = 1.706e-14
Identity = 41/121 (33.88%), Postives = 72/121 (59.50%), Query Frame = 3 
Query:  261 FNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNF-DAEIIESSKWDSDE 620
            F+ +S + +  N+LK    H  +++     QI +LL +++DK+KG   + +  +N+    ++PD++LN + ++ L + KA MD  F  N+ +P D DF+YD E +F DA  IES  WD ++
Sbjct:   38 FDGDSSIQNTANELKKSSHHGKFIEGIPQKQIEKLLKLIQDKIKGLEPKPNPTENKPAAQLDPDKNLNKLGDDELNKVKAAMDIDFEKNRIRPGDEDFEYDKEVDFGDAGKIESG-WDEED 157
[Close]
BLAST of Centrosomal protein of 19 kDa vs. Ensembl Medaka
Match: cep19 (centrosomal protein 19 [Source:NCBI gene;Acc:101167027])

HSP 1 Score: 75.8702 bits (185), Expect = 7.431e-17
Identity = 45/113 (39.82%), Postives = 64/113 (56.64%), Query Frame = 3 
Query:  297 QLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDAEIIESSKWDSDEGDSGF 635
            QLK    H  YL+     Q+ +L  +++D L+G SL+ S+        ++PDEDLN +D++ L RKKA MD  F  N+K   DP F YD+E +F+    E+  W  DE D GF
Sbjct:   51 QLKEHPRHRDYLEAVSQGQLEKLHILLQDHLRGLSLKHSLSTFH----LDPDEDLNKLDDKELARKKAQMDREFERNRKHKDDPGFVYDLEVDFEKAPQENCSW--DESDDGF 157
[Close]
BLAST of Centrosomal protein of 19 kDa vs. Planmine SMEST
Match: SMESG000010236.1 (SMESG000010236.1)

HSP 1 Score: 280.796 bits (717), Expect = 6.215e-97
Identity = 164/165 (99.39%), Postives = 164/165 (99.39%), Query Frame = 3 
Query:  141 MNNVINVQKVGIKFHEKIANXXXXXXXXXXXXXXXXXXXXFNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDAEIIESSKWDSDEGDSGF 635
            MNNVINVQKVGIKFHEKIANLILIYKEGKKLKKHEIKLIGFNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNR IDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDAEIIESSKWDSDEGDSGF
Sbjct:    1 MNNVINVQKVGIKFHEKIANLILIYKEGKKLKKHEIKLIGFNENSVVIDYINQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRSIDVINPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDAEIIESSKWDSDEGDSGF 165
The following BLAST results are available for this feature:
BLAST of Centrosomal protein of 19 kDa vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CEP193.100e-1933.13centrosomal protein 19 [Source:HGNC Symbol;Acc:HGN... [more]
CEP191.326e-1839.64centrosomal protein 19 [Source:HGNC Symbol;Acc:HGN... [more]
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BLAST of Centrosomal protein of 19 kDa vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Centrosomal protein of 19 kDa vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Centrosomal protein of 19 kDa vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
cep191.038e-1134.92centrosomal protein 19 [Source:NCBI gene;Acc:61925... [more]
cep191.038e-1134.92centrosomal protein 19 [Source:NCBI gene;Acc:61925... [more]
cep191.038e-1134.92centrosomal protein 19 [Source:NCBI gene;Acc:61925... [more]
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BLAST of Centrosomal protein of 19 kDa vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
cep191.863e-1839.68centrosomal protein 19kDa [Source:NCBI gene;Acc:10... [more]
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BLAST of Centrosomal protein of 19 kDa vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Cep194.705e-1736.59centrosomal protein 19 [Source:MGI Symbol;Acc:MGI:... [more]
Cep194.705e-1736.59centrosomal protein 19 [Source:MGI Symbol;Acc:MGI:... [more]
Cep194.705e-1736.59centrosomal protein 19 [Source:MGI Symbol;Acc:MGI:... [more]
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BLAST of Centrosomal protein of 19 kDa vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q96LK0|CEP19_HUMAN7.523e-1833.13Centrosomal protein of 19 kDa OS=Homo sapiens OX=9... [more]
sp|A9UL78|CEP19_XENTR9.868e-1839.68Centrosomal protein of 19 kDa OS=Xenopus tropicali... [more]
sp|A6H7C9|CEP19_BOVIN1.826e-1733.13Centrosomal protein of 19 kDa OS=Bos taurus OX=991... [more]
sp|Q9QZX9|CEP19_RAT2.128e-1636.13Centrosomal protein of 19 kDa OS=Rattus norvegicus... [more]
sp|Q9CQA8|CEP19_MOUSE3.291e-1636.59Centrosomal protein of 19 kDa OS=Mus musculus OX=1... [more]
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BLAST of Centrosomal protein of 19 kDa vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A433SLU72.990e-3241.82Uncharacterized protein OS=Elysia chlorotica OX=18... [more]
K1QZR98.072e-3046.40UPF0709 protein C3orf34 OS=Crassostrea gigas OX=29... [more]
R7TP074.775e-2847.20Uncharacterized protein OS=Capitella teleta OX=283... [more]
A0A2T7ND908.393e-2746.34Uncharacterized protein OS=Pomacea canaliculata OX... [more]
A0A2R2MSZ01.844e-2536.77centrosomal protein of 19 kDa OS=Lingula unguis OX... [more]
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BLAST of Centrosomal protein of 19 kDa vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
cep194.515e-1839.47centrosomal protein 19 [Source:NCBI gene;Acc:10302... [more]
cep194.515e-1839.47centrosomal protein 19 [Source:NCBI gene;Acc:10302... [more]
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BLAST of Centrosomal protein of 19 kDa vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Centrosomal protein of 19 kDa vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Centrosomal protein of 19 kDa vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO418371.706e-1433.88Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Centrosomal protein of 19 kDa vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
cep197.431e-1739.82centrosomal protein 19 [Source:NCBI gene;Acc:10116... [more]
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BLAST of Centrosomal protein of 19 kDa vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000010236.16.215e-9799.39SMESG000010236.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011543 ID=SMED30011543|Name=Centrosomal protein of 19 kDa|organism=Schmidtea mediterranea sexual|type=transcript|length=737bp
CTATGATAACTAAAAGCGCAATATTCAAATAATAATTTTATCCATATTTA
GCTGTTATTTTATAAATTGTAATTAGTAATTCAAAAATTAATTAAATTCT
AATAAGTAATTGAATCAAATCATATTTAATATCGGTTTAAATGAATAACG
TAATTAACGTTCAAAAAGTTGGAATTAAATTCCATGAAAAAATTGCGAAT
TTAATTTTAATCTACAAAGAAGGCAAGAAGCTTAAAAAACATGAAATTAA
ATTAATTGGATTTAATGAAAACAGCGTAGTAATTGATTATATAAATCAGC
TCAAATCCCAGGAGGCTCATGAACCATATCTGAAAAATACCGTCGATTTG
CAAATCATACGTTTATTGAGTATCGTGCGTGATAAATTGAAAGGATTTTC
ATTGCAAGATAGTATTGAAAAGAACCGAGGCATCGATGTAATTAATCCAG
ATGAAGATCTGAATGTGGTCGATGAGGAGACATTATTCCGGAAAAAAGCC
GTTATGGATGAAACGTTTCTCACCAATCAAAAGAAACCGACAGATCCGGA
TTTCCAGTATGACATTGAGAAAAATTTCGACGCGGAAATAATCGAGTCCA
GCAAATGGGATTCAGACGAGGGCGATTCTGGATTTTAATAAAATATTGTC
TTAATTCTTTTATCGATTAACTGCCACATCAATTTATGTAATTTTAATTG
TATATGTGTGTACTGTGTGTGTTGTGCATGCTTGTAT
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protein sequence of SMED30011543-orf-1

>SMED30011543-orf-1 ID=SMED30011543-orf-1|Name=SMED30011543-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=166bp
MNNVINVQKVGIKFHEKIANLILIYKEGKKLKKHEIKLIGFNENSVVIDY
INQLKSQEAHEPYLKNTVDLQIIRLLSIVRDKLKGFSLQDSIEKNRGIDV
INPDEDLNVVDEETLFRKKAVMDETFLTNQKKPTDPDFQYDIEKNFDAEI
IESSKWDSDEGDSGF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000499cell
PLANA:0000418head
Vocabulary: cellular component
TermDefinition
GO:0005929cilium
GO:0036064ciliary basal body
GO:0000922spindle pole
GO:0005737cytoplasm
GO:0005814centriole
GO:0005856cytoskeleton
GO:0042995cell projection
Vocabulary: biological process
TermDefinition
GO:0030030cell projection organization
GO:0060271cilium morphogenesis
Vocabulary: INTERPRO
TermDefinition
IPR029412CEP19
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029412Centrosomal protein of 19kDaPFAMPF14933CEP19coord: 9..157
e-value: 2.2E-27
score: 96.4
IPR029412Centrosomal protein of 19kDaPANTHERPTHR31539FAMILY NOT NAMEDcoord: 104..161
coord: 8..102