CRAL-TRIO domain-containing protein

Overview
NameCRAL-TRIO domain-containing protein
Smed IDSMED30011535
Length (bp)867
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of CRAL-TRIO domain-containing protein (SMED30011535) t-SNE clustered cells

Violin plots show distribution of expression levels for CRAL-TRIO domain-containing protein (SMED30011535) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of CRAL-TRIO domain-containing protein (SMED30011535) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for CRAL-TRIO domain-containing protein (SMED30011535) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30011535

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30011535h1SMcG0021902 dd_Smed_v4_14557_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
reproductive organSMED30011535h1SMcG0021902 Contig1249GPL15192PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
zeta neoblastSMED30011535h1SMcG0021902 dd_Smed_v4_14557_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Human
Match: MOSPD2 (motile sperm domain containing 2 [Source:HGNC Symbol;Acc:HGNC:28381])

HSP 1 Score: 68.9366 bits (167), Expect = 1.462e-12
Identity = 49/166 (29.52%), Postives = 85/166 (51.20%), Query Frame = 2 
Query:  275 WRKEFGVXXXXXXXXXXQI-EINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHE---QISLYFDPKHL 760
            WRKE  V++L+ + I   + EI    V+ L   DK    + +I VK H+   + I    K + + +ER  ++  EN   + ++FDL+   I ++D  FVR +I+ F  YYP+ L   +I + P +    + +VK WL    V  + F + +E    +S+ + P H+
Sbjct:   71 WRKEISVNDLNESSIPRWLLEIG---VIYLHGYDKEGNKLFWIRVKYHVKDQKTILDKKKLIAFWLERYAKR--ENGKPVTVMFDLSETGINSIDMDFVRFIINCFKVYYPKYLSKIVIFDMPWLMNAAFKIVKTWLGPEAVSLLKFTSKNEVQDYVSVEYLPPHM 231
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Human
Match: MOSPD2 (motile sperm domain containing 2 [Source:HGNC Symbol;Acc:HGNC:28381])

HSP 1 Score: 68.5514 bits (166), Expect = 2.111e-12
Identity = 49/166 (29.52%), Postives = 85/166 (51.20%), Query Frame = 2 
Query:  275 WRKEFGVXXXXXXXXXXQI-EINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHE---QISLYFDPKHL 760
            WRKE  V++L+ + I   + EI    V+ L   DK    + +I VK H+   + I    K + + +ER  ++  EN   + ++FDL+   I ++D  FVR +I+ F  YYP+ L   +I + P +    + +VK WL    V  + F + +E    +S+ + P H+
Sbjct:   71 WRKEISVNDLNESSIPRWLLEIG---VIYLHGYDKEGNKLFWIRVKYHVKDQKTILDKKKLIAFWLERYAKR--ENGKPVTVMFDLSETGINSIDMDFVRFIINCFKVYYPKYLSKIVIFDMPWLMNAAFKIVKTWLGPEAVSLLKFTSKNEVQDYVSVEYLPPHM 231
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Celegans
Match: T03F7.7 (pep chromosome:WBcel235:V:11311456:11313445:1 gene:WBGene00011404.1 transcript:T03F7.7a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T03F7.7)

HSP 1 Score: 49.6766 bits (117), Expect = 1.242e-6
Identity = 52/211 (24.64%), Postives = 92/211 (43.60%), Query Frame = 2 
Query:  209 TRFIRAF-GSASIAYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVLILPHLDKLNRPVVYISVKNHI--VQNRNIDSVTKFM--VYVI----------ERA---CEKCIENQDQLCIIFDLNSFSIGNMDY--------AFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLP 763
             RF+ A  G      Q+I++    RK  G + ++  DI   + I K C     H+ +LN  V   ++   +  ++  +I  + K M   YV+           RA    E+       +  I DL   ++  MD+           R ++ +++ Y+ E L   L+IN P I    W V KR +  +TV+K+ F ++ E +  Y +P+ +P
Sbjct:   40 ARFLNANNGDVEKTRQKIIQLFEHRKLMGYEKINDLDIFTTVNIGKDCFERF-HISQLNYEVTSKNLHVFVQKMEGTDIKEILKVMPLSYVLHSYFMLQENFSRAMAHTERKTGKTSSVVCILDLKGLNL--MDFMNPLSGPAQLARLVVQVWAEYFSEHLCKLLLINPPGIISVMWQVTKRLVDPNTVEKLAFLSNVEDLKKYLEPEAIP 247
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Celegans
Match: T03F7.7 (pep chromosome:WBcel235:V:11311456:11313445:1 gene:WBGene00011404.1 transcript:T03F7.7b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T03F7.7)

HSP 1 Score: 49.2914 bits (116), Expect = 1.616e-6
Identity = 52/211 (24.64%), Postives = 92/211 (43.60%), Query Frame = 2 
Query:  209 TRFIRAF-GSASIAYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVLILPHLDKLNRPVVYISVKNHI--VQNRNIDSVTKFM--VYVI----------ERA---CEKCIENQDQLCIIFDLNSFSIGNMDYA--------FVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLP 763
             RF+ A  G      Q+I++    RK  G + ++  DI   + I K C     H+ +LN  V   ++   +  ++  +I  + K M   YV+           RA    E+       +  I DL   ++  MD+           R ++ +++ Y+ E L   L+IN P I    W V KR +  +TV+K+ F ++ E +  Y +P+ +P
Sbjct:   40 ARFLNANNGDVEKTRQKIIQLFEHRKLMGYEKINDLDIFTTVNIGKDCFERF-HISQLNYEVTSKNLHVFVQKMEGTDIKEILKVMPLSYVLHSYFMLQENFSRAMAHTERKTGKTSSVVCILDLKGLNL--MDFMNPLSGPAQLARLVVQVWAEYFSEHLCKLLLINPPGIISVMWQVTKRLVDPNTVEKLAFLSNVEDLKKYLEPEAIP 247
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Celegans
Match: hpo-28 (Major sperm protein [Source:UniProtKB/TrEMBL;Acc:Q10956])

HSP 1 Score: 47.3654 bits (111), Expect = 8.628e-6
Identity = 39/166 (23.49%), Postives = 69/166 (41.57%), Query Frame = 2 
Query:  242 IAYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNI------------DSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQK 703
            IAY  +++ + WR+ F              E+++  +L L  L  L+  ++Y+  K+  +QNR++            D   K   + IER   +    Q  L +  D+    + NM +  ++ +IH    YYP  +   LI  +P I    W V+  WL      +
Sbjct:   72 IAYAIMLECLKWRRNF--------------EVDRISLLSLKPL--LDNQLMYLHGKD--LQNRHMLWIMMNKYKNGDDGFEKLFTFWIERHYMEYKGCQ-PLTVFIDMTGTGLKNMSFDAMKFIIHSSKYYYPNSIESILIFENPAILNASWKVIGSWLESSAASQ 218
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Fly
Match: CG32485 (gene:FBgn0052485 transcript:FBtr0072985)

HSP 1 Score: 151.754 bits (382), Expect = 9.055e-45
Identity = 81/187 (43.32%), Postives = 117/187 (62.57%), Query Frame = 2 
Query:  212 RFIRAFGSASIAYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIEN-QDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLPSN 769
            R++RAF +   A+Q I+K   WR+ +GVD L   D   + +++KK  L L H D + RPV+YI  KNH  + R+ID +T+F+VY +E AC+KC E   D+LCI+FDL  FS   MDY  V+ LI L   ++PERLG+CLIINSP +F   W  ++  L D+T +K+ F     ++  Y  P  LP++
Sbjct:   40 RYLRAFKTTDDAFQAILKTNKWRETYGVDKLSEMD---RSQLDKKARL-LRHRDCIGRPVIYIPAKNHSSE-RDIDELTRFIVYNLEEACKKCFEEVTDRLCIVFDLAEFSTSCMDYQLVQNLIWLLGKHFPERLGVCLIINSPGLFSTIWPAIRVLLDDNTAKKVKFVADEAELCQYLIPDILPTD 221
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Fly
Match: CG32407 (gene:FBgn0052407 transcript:FBtr0330118)

HSP 1 Score: 63.5438 bits (153), Expect = 1.774e-11
Identity = 40/165 (24.24%), Postives = 85/165 (51.52%), Query Frame = 2 
Query:  254 RIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFD 748
            R+   + WRK FGV ++   +++ +  +N   + +  + D+  +P++ +++K H  ++RN + + + +V+ IER       N D++ I  D+    + N+D  F++ +I +F T YP      L+ + P +    + +VK +L    + KI+   + + I  Y D
Sbjct:   64 RLWDNLAWRKSFGVYDITEANLNQEF-LNDGSIYVH-NKDRDGKPLLILTIKKH-SKSRNQEDLLRILVFWIERLQRDS--NLDKITIFMDMTGAGLSNLDMGFIKSIIGVFETKYPYVPNYILVHDLPFLLDAAFKLVKTFLPPEAL-KILKVTTKKDIDQYVD 222
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Fly
Match: CG32407 (gene:FBgn0052407 transcript:FBtr0077032)

HSP 1 Score: 63.5438 bits (153), Expect = 1.774e-11
Identity = 40/165 (24.24%), Postives = 85/165 (51.52%), Query Frame = 2 
Query:  254 RIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFD 748
            R+   + WRK FGV ++   +++ +  +N   + +  + D+  +P++ +++K H  ++RN + + + +V+ IER       N D++ I  D+    + N+D  F++ +I +F T YP      L+ + P +    + +VK +L    + KI+   + + I  Y D
Sbjct:   64 RLWDNLAWRKSFGVYDITEANLNQEF-LNDGSIYVH-NKDRDGKPLLILTIKKH-SKSRNQEDLLRILVFWIERLQRDS--NLDKITIFMDMTGAGLSNLDMGFIKSIIGVFETKYPYVPNYILVHDLPFLLDAAFKLVKTFLPPEAL-KILKVTTKKDIDQYVD 222
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Fly
Match: retm (gene:FBgn0031814 transcript:FBtr0329951)

HSP 1 Score: 60.8474 bits (146), Expect = 3.836e-10
Identity = 48/201 (23.88%), Postives = 97/201 (48.26%), Query Frame = 2 
Query:  239 SIAYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVLI--LP----HLDKLNRPVV-----YISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQL-------CIIFDLNSFSIGNMDYAFVRQLIHLFSTY---YPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIF-----CNSHEQISLYFDPKHLP 763
            S AY  +   + WR+E  +D L         E +K  V++   P    HLDK  RPV      ++ VK  ++++  +D + +  +++ E   +K  E+ ++L        ++ DL   S+ ++    ++ L+++  T    YPE +G  L++ +P +F   WT+V  ++ +HT  K +F      +  + ++ Y D + +P
Sbjct:  259 SQAYAMLCDSLRWRREHRIDALLA-------EYSKPAVVVEHFPGGWHHLDKDGRPVYILRLGHMDVKG-LLKSLGMDGLLRLALHICEEGIQKINESAERLEKPVLNWSLLVDLEGLSMRHLWRPGIKALLNIIETVERNYPETMGRVLVVRAPRVFPIAWTIVSAFIDEHTRSKFLFYGPDCAHMKDGLAQYLDEEIVP 451
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Fly
Match: retm (gene:FBgn0031814 transcript:FBtr0079272)

HSP 1 Score: 60.8474 bits (146), Expect = 4.451e-10
Identity = 48/201 (23.88%), Postives = 97/201 (48.26%), Query Frame = 2 
Query:  239 SIAYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVLI--LP----HLDKLNRPVV-----YISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQL-------CIIFDLNSFSIGNMDYAFVRQLIHLFSTY---YPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIF-----CNSHEQISLYFDPKHLP 763
            S AY  +   + WR+E  +D L         E +K  V++   P    HLDK  RPV      ++ VK  ++++  +D + +  +++ E   +K  E+ ++L        ++ DL   S+ ++    ++ L+++  T    YPE +G  L++ +P +F   WT+V  ++ +HT  K +F      +  + ++ Y D + +P
Sbjct:  259 SQAYAMLCDSLRWRREHRIDALLA-------EYSKPAVVVEHFPGGWHHLDKDGRPVYILRLGHMDVKG-LLKSLGMDGLLRLALHICEEGIQKINESAERLEKPVLNWSLLVDLEGLSMRHLWRPGIKALLNIIETVERNYPETMGRVLVVRAPRVFPIAWTIVSAFIDEHTRSKFLFYGPDCAHMKDGLAQYLDEEIVP 451
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Zebrafish
Match: mospd2 (motile sperm domain containing 2 [Source:ZFIN;Acc:ZDB-GENE-041114-1])

HSP 1 Score: 74.3294 bits (181), Expect = 1.537e-14
Identity = 48/170 (28.24%), Postives = 83/170 (48.82%), Query Frame = 2 
Query:  257 IVKYILWRKEFGVXXXXXXXXXXQI-EINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLP 763
            I + + WR+EF V++L  + I   + EI     + L   DK    + +  VK HI   + +    +++ + +ER  ++  E    L ++FD++   + N+D  FV+ +I  F  YYP+ L   ++   P I    W +VK WL    + K+ F  S   I  +  P+HLP
Sbjct:   71 IDESLQWRREFSVNDLTESSIPRWMFEIG---AVYLHGYDKEGNKLFWFKVKLHIKDPKTVLDKKRYVAFWLERYAKR--EPGMPLTVVFDMSESGLSNIDMDFVKYIISCFKVYYPKFLSKMIMYEMPWIMNAAWKIVKTWLGPDAISKLKFV-SKSDIQTFVGPEHLP 234
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Zebrafish
Match: mospd2 (motile sperm domain containing 2 [Source:ZFIN;Acc:ZDB-GENE-041114-1])

HSP 1 Score: 74.3294 bits (181), Expect = 1.671e-14
Identity = 48/170 (28.24%), Postives = 83/170 (48.82%), Query Frame = 2 
Query:  257 IVKYILWRKEFGVXXXXXXXXXXQI-EINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLP 763
            I + + WR+EF V++L  + I   + EI     + L   DK    + +  VK HI   + +    +++ + +ER  ++  E    L ++FD++   + N+D  FV+ +I  F  YYP+ L   ++   P I    W +VK WL    + K+ F  S   I  +  P+HLP
Sbjct:   71 IDESLQWRREFSVNDLTESSIPRWMFEIG---AVYLHGYDKEGNKLFWFKVKLHIKDPKTVLDKKRYVAFWLERYAKR--EPGMPLTVVFDMSESGLSNIDMDFVKYIISCFKVYYPKFLSKMIMYEMPWIMNAAWKIVKTWLGPDAISKLKFV-SKSDIQTFVGPEHLP 234
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Zebrafish
Match: ptpn9b (protein tyrosine phosphatase non-receptor type 9b [Source:ZFIN;Acc:ZDB-GENE-090313-52])

HSP 1 Score: 56.9954 bits (136), Expect = 1.197e-8
Identity = 31/138 (22.46%), Postives = 67/138 (48.55%), Query Frame = 2 
Query:  356 ILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHE---QISLYFDPKHL 760
            +LP  D     +   + + H        +V + ++Y +++A E     +D L  I+D+ + +  N DY    ++++L    +P RL    I++SPL F+  + V++ ++ +   +++    +HE    I +   P+HL
Sbjct:   87 VLPGRDAKGAALALFTARLHQPDRTTHKAVLQAIIYQLDKAIESVQTQRDGLIFIYDMTNSTYANFDYELCVKILNLLKGAFPARLKCVFIVSSPLWFRAPFAVLRLFVREKLRERVCTVKAHELVNHIPVSSLPEHL 224
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Zebrafish
Match: sec14l8 (SEC14-like lipid binding 8 [Source:ZFIN;Acc:ZDB-GENE-060526-180])

HSP 1 Score: 48.521 bits (114), Expect = 4.402e-6
Identity = 26/104 (25.00%), Postives = 54/104 (51.92%), Query Frame = 2 
Query:  470 ERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVR---QLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSH--EQISLYFDPKHLPS 766
            +R  E+   N + + +++D     + ++    +    +++ +F   YPE L    +I +P +F   + +VK +LS+ T +K+I   S+  E +  Y DP+ LP+
Sbjct:  137 DRQSERLGRNIESITMVYDCEGLGMKHLYKPAIETYGEVLTMFEDNYPEGLKRLFVIKAPKLFPVAYNLVKHFLSEDTRRKVIVLGSNWQEVLQKYIDPEELPA 240
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Xenopus
Match: mospd2 (motile sperm domain containing 2 [Source:NCBI gene;Acc:493504])

HSP 1 Score: 72.0182 bits (175), Expect = 1.347e-13
Identity = 51/200 (25.50%), Postives = 93/200 (46.50%), Query Frame = 2 
Query:  176 EDINKVDVLTQTRFIRAFGSASIAYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKC----VLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLP 763
            E + K D L ++  +        A + + + + WRK+ GV++L+      +  I K C       L   DK    ++++ VK H+   +  +   KF+ + +ER   +  E    + ++FD+    + N+D  FVR +++ F TYYP  L   +I   P I    + +VK WL    +  + F N + Q+  Y   ++LP
Sbjct:   34 ERLQKDDTLVESYLMWRHYVTEEALKMMDESLKWRKDIGVNDLN------ESTIPKWCFETGASYLHGYDKEGNKLLWLKVKLHVRDGKTTEDKKKFVAFWLERYARR--EPGKFITVVFDMVDSGLSNVDMDFVRFVVNSFKTYYPRYLSKMVIYEMPWILNAAFKIVKSWLGPEAISLLKFANKN-QVQDYISAEYLP 224
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Xenopus
Match: mospd2 (motile sperm domain containing 2 [Source:NCBI gene;Acc:493504])

HSP 1 Score: 71.633 bits (174), Expect = 1.755e-13
Identity = 51/200 (25.50%), Postives = 93/200 (46.50%), Query Frame = 2 
Query:  176 EDINKVDVLTQTRFIRAFGSASIAYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKC----VLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLP 763
            E + K D L ++  +        A + + + + WRK+ GV++L+      +  I K C       L   DK    ++++ VK H+   +  +   KF+ + +ER   +  E    + ++FD+    + N+D  FVR +++ F TYYP  L   +I   P I    + +VK WL    +  + F N + Q+  Y   ++LP
Sbjct:   38 ERLQKDDTLVESYLMWRHYVTEEALKMMDESLKWRKDIGVNDLN------ESTIPKWCFETGASYLHGYDKEGNKLLWLKVKLHVRDGKTTEDKKKFVAFWLERYARR--EPGKFITVVFDMVDSGLSNVDMDFVRFVVNSFKTYYPRYLSKMVIYEMPWILNAAFKIVKSWLGPEAISLLKFANKN-QVQDYISAEYLP 228
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Xenopus
Match: mospd2 (motile sperm domain containing 2 [Source:NCBI gene;Acc:493504])

HSP 1 Score: 67.3958 bits (163), Expect = 4.617e-12
Identity = 51/200 (25.50%), Postives = 92/200 (46.00%), Query Frame = 2 
Query:  176 EDINKVDVLTQTRFIRAFGSASIAYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKC----VLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLP 763
            E + K D L ++  +        A + + + + WRK+ GV++L+      +  I K C       L   DK    ++++ VK H+   +  +   KF+ + +ER   +  E    + ++FD+    + N D  FVR +++ F TYYP  L   +I   P I    + +VK WL    +  + F N + Q+  Y   ++LP
Sbjct:   19 ERLQKDDTLVESYLMWRHYVTEEALKMMDESLKWRKDIGVNDLN------ESTIPKWCFETGASYLHGYDKEGNKLLWLKVKLHVRDGKTTEDKKKFVAFWLERYARR--EPGKFITVVFDMVDSGLSN-DMDFVRFVVNSFKTYYPRYLSKMVIYEMPWILNAAFKIVKSWLGPEAISLLKFANKN-QVQDYISAEYLP 208
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Xenopus
Match: mfhas1 (malignant fibrous histiocytoma amplified sequence 1 [Source:Xenbase;Acc:XB-GENE-1003080])

HSP 1 Score: 55.4546 bits (132), Expect = 4.486e-8
Identity = 29/139 (20.86%), Postives = 69/139 (49.64%), Query Frame = 2 
Query:  356 ILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLPSNN 772
            +LP  D     +   + + H        +V + ++Y +++A E+    ++ L  I+D+ + + GN DY    ++++L    +P RL    I++SPL F+  + V++ ++ +   +++    +HE ++      H+P  +
Sbjct:   88 VLPGRDANGAALALFTARLHRPDVTTHKAVLQAIIYQLDKAIERVETQRNGLIFIYDMINSTYGNFDYELCVKILNLLKGAFPARLKCVFIVSSPLWFRAPFAVLRLFVREKLRERVRTVKAHELVN------HIPKES 220
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Xenopus
Match: mfhas1 (malignant fibrous histiocytoma amplified sequence 1 [Source:Xenbase;Acc:XB-GENE-1003080])

HSP 1 Score: 55.4546 bits (132), Expect = 4.550e-8
Identity = 29/139 (20.86%), Postives = 69/139 (49.64%), Query Frame = 2 
Query:  356 ILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLPSNN 772
            +LP  D     +   + + H        +V + ++Y +++A E+    ++ L  I+D+ + + GN DY    ++++L    +P RL    I++SPL F+  + V++ ++ +   +++    +HE ++      H+P  +
Sbjct:   88 VLPGRDANGAALALFTARLHRPDVTTHKAVLQAIIYQLDKAIERVETQRNGLIFIYDMINSTYGNFDYELCVKILNLLKGAFPARLKCVFIVSSPLWFRAPFAVLRLFVREKLRERVRTVKAHELVN------HIPKES 220
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Mouse
Match: Mospd2 (motile sperm domain containing 2 [Source:MGI Symbol;Acc:MGI:1924013])

HSP 1 Score: 69.707 bits (169), Expect = 5.767e-13
Identity = 48/166 (28.92%), Postives = 84/166 (50.60%), Query Frame = 2 
Query:  275 WRKEFGVXXXXXXXXXXQI-EINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSH---EQISLYFDPKHL 760
            WRKEF V++L  + I   + E+     + L   DK    + +I VK HI   + I    K + + +ER  ++  EN   + ++FD++   + ++D  FVR +I+ F  YYP+ L   +I + P I    + +VK WL    V  + F + +   E +S+ + P H+
Sbjct:   71 WRKEFSVNDLSESSIPRWLLELGG---IYLHGYDKEGNKLFWIRVKYHIKDQKTIMDKKKLIAFWLERYAKR--ENGKPITVMFDMSETGLNSIDMDFVRFIINCFKVYYPKYLSKIVIFDMPWIMNAAFKIVKSWLGPEAVSLLKFTSKNEIQEYVSVEYLPPHM 231
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Mouse
Match: Mospd2 (motile sperm domain containing 2 [Source:MGI Symbol;Acc:MGI:1924013])

HSP 1 Score: 69.3218 bits (168), Expect = 7.047e-13
Identity = 48/166 (28.92%), Postives = 84/166 (50.60%), Query Frame = 2 
Query:  275 WRKEFGVXXXXXXXXXXQI-EINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSH---EQISLYFDPKHL 760
            WRKEF V++L  + I   + E+     + L   DK    + +I VK HI   + I    K + + +ER  ++  EN   + ++FD++   + ++D  FVR +I+ F  YYP+ L   +I + P I    + +VK WL    V  + F + +   E +S+ + P H+
Sbjct:   71 WRKEFSVNDLSESSIPRWLLELGG---IYLHGYDKEGNKLFWIRVKYHIKDQKTIMDKKKLIAFWLERYAKR--ENGKPITVMFDMSETGLNSIDMDFVRFIINCFKVYYPKYLSKIVIFDMPWIMNAAFKIVKSWLGPEAVSLLKFTSKNEIQEYVSVEYLPPHM 231
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Mouse
Match: Mospd2 (motile sperm domain containing 2 [Source:MGI Symbol;Acc:MGI:1924013])

HSP 1 Score: 69.3218 bits (168), Expect = 8.040e-13
Identity = 48/166 (28.92%), Postives = 84/166 (50.60%), Query Frame = 2 
Query:  275 WRKEFGVXXXXXXXXXXQI-EINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSH---EQISLYFDPKHL 760
            WRKEF V++L  + I   + E+     + L   DK    + +I VK HI   + I    K + + +ER  ++  EN   + ++FD++   + ++D  FVR +I+ F  YYP+ L   +I + P I    + +VK WL    V  + F + +   E +S+ + P H+
Sbjct:   71 WRKEFSVNDLSESSIPRWLLELGG---IYLHGYDKEGNKLFWIRVKYHIKDQKTIMDKKKLIAFWLERYAKR--ENGKPITVMFDMSETGLNSIDMDFVRFIINCFKVYYPKYLSKIVIFDMPWIMNAAFKIVKSWLGPEAVSLLKFTSKNEIQEYVSVEYLPPHM 231
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. UniProt/SwissProt
Match: sp|Q10138|YAS2_SCHPO (CRAL-TRIO domain-containing protein C3H8.02 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC3H8.02 PE=1 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 7.103e-22
Identity = 45/130 (34.62%), Postives = 75/130 (57.69%), Query Frame = 2 
Query:  371 DKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHL 760
            DK NRPV YI  + H V + + +SV +  V+V+E A        +   ++FD+  FS+ NMDY  ++ +I  F  +YPE LG C++  +P +FQG W+++K WL    V K+ F  ++  +  Y +P ++
Sbjct:  189 DKHNRPVCYIRARLHKVGDVSPESVERLTVWVMETARLILKPPIETATVVFDMTDFSMSNMDYGPLKFMIKCFEAHYPECLGECIVHKAPWLFQGVWSIIKSWLDPVVVSKVKFTRNYRDLQQYINPDNI 318
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9HDZ5|YKP9_SCHPO (CRAL-TRIO domain-containing protein C589.09, mitochondrial OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC589.09 PE=4 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.870e-20
Identity = 52/192 (27.08%), Postives = 93/192 (48.44%), Query Frame = 2 
Query:  245 AYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVL--------ILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIE---------RACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLPSN 769
            A Q+++  + WR + GV+ +         E N    L         +   D  +RP+ YI V  H       +S+ +  V+V+E         +  +  +++   + ++FDL++FS+ NMDY+FV+ L      YYP+ LG+C++  SP IF+  W ++K W+      KI+F  S   +  Y D   +P++
Sbjct:   97 ASQKLINTLQWRVDTGVERIVERGELYAKETNDDQFLEQLRTGKVTMLGRDLSDRPICYIQVHLHQPSKLTQNSLREMTVWVMETMRLFLRPQKTLKDSMDSPQNVNVLFDLSNFSLHNMDYSFVKYLASCLEYYYPQSLGVCILHKSPWIFRSVWNIIKGWIKPEIAAKIVFTQSANDLEKYIDYSVIPTS 288
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. UniProt/SwissProt
Match: sp|Q06705|CSR1_YEAST (Phosphatidylinositol transfer protein CSR1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CSR1 PE=1 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.978e-18
Identity = 46/128 (35.94%), Postives = 71/128 (55.47%), Query Frame = 2 
Query:  383 RPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIEN-QDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLP 763
            RPV+ +  + H   ++    + KF + VIE++     EN      I+FDLN FS+ NMDYA V+ LI  F  +YPE LG  LI  +P IF   W ++K WL      KI+F  + +++  +  P+++P
Sbjct:  179 RPVILVRPRLHHSSDQTEQELEKFSLLVIEQSKLFFKENYPASTTILFDLNGFSMSNMDYAPVKFLITCFEAHYPESLGHLLIHKAPWIFNPIWNIIKNWLDPVVASKIVFTKNIDELHKFIQPQYIP 306
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. UniProt/SwissProt
Match: sp|Q757H2|CSR1_ASHGO (Phosphatidylinositol transfer protein CSR1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=CSR1 PE=3 SV=2)

HSP 1 Score: 86.6557 bits (213), Expect = 5.837e-18
Identity = 52/165 (31.52%), Postives = 80/165 (48.48%), Query Frame = 2 
Query:  269 ILWRKEFGVXXXXXXXXXXQIEINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLP 763
            I++  E G    D   I  Q+E+ K  V      DK   P+VY+  + H   ++     +++ + +IE+A     E  D   I+FDL+ FS+ NMDYA V+ LI  F  +YPE LG   I  +P IF   W ++K WL      KI F  +   +  +   + +P
Sbjct:  175 IVFGGERGAQKADKKGIVKQLELGKATVR---GFDKNGCPIVYVRPRLHHAADQTEAETSEYSLLIIEQARLFLKEPCDTATILFDLSGFSMANMDYAPVKFLITCFEAHYPECLGKLFIHKAPWIFPPIWNIIKNWLDPVVAAKIAFTKTAADLEEFIPAEQIP 336
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. UniProt/SwissProt
Match: sp|Q55CU8|PITC_DICDI (Phosphatidylinositol transfer protein 3 OS=Dictyostelium discoideum OX=44689 GN=pitC PE=2 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 5.374e-13
Identity = 47/175 (26.86%), Postives = 88/175 (50.29%), Query Frame = 2 
Query:  212 RFIRAFGSASIAYQRIVKYIL-WRKEFGVXXXXXXXXXXQIEINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQ--DQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHE 727
            R++RA        +++++  L WRK+F   ++ L     +I  +  CV +    DK  RP+++   +N  ++N   +   K +VY +E+   +  E +  +Q C I D   F  GNMD     + +H    + PER+G  L ++ P +F   W ++  +L++ T+ K+ F NS +
Sbjct:   78 RYLRARNYIVSKSEKMLRDTLEWRKKFRPQDIQLGGDIREIG-SAGCVYVNKR-DKKGRPIIFAVPRNDTLKNVPSELKFKNLVYWLEQGFSRMDEPKGIEQFCFIVDYKDFGSGNMDMKTNLEAMHFLLDHCPERMGQSLFLDPPALFWFAWKIISPFLNEVTLSKVRFINSKK 250
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. TrEMBL
Match: A0A293LCF1 (CRAL-TRIO domain-containing protein (Fragment) OS=Ornithodoros erraticus OX=265619 PE=4 SV=1)

HSP 1 Score: 185.267 bits (469), Expect = 2.813e-54
Identity = 89/187 (47.59%), Postives = 124/187 (66.31%), Query Frame = 2 
Query:  209 TRFIRAFGSASIAYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQ-DQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLPS 766
            TRF+RAF +   A+Q I+K   WR E+GV  +  N  DI+  +  K  ++L + D   RPVVYI  + H VQ+R+ID +T+F+VY++E AC+KC E   D LCIIFDL  F + +MDY  ++ LI L S +YPERLG+CL++NSP IF GCW V++ WL D T +K++F NS E++  Y  P  LP 
Sbjct:   41 TRFLRAFRTVDNAFQAILKCNKWRSEYGVKTITPNQPDIKKNLESKKAMVLGNRDFCGRPVVYIPARKHNVQDRDIDELTRFIVYILEEACKKCFEEVIDNLCIIFDLKDFGLNSMDYPLIKNLIWLLSKHYPERLGVCLVLNSPSIFSGCWVVIRGWLDDVTARKVVFINSQEELVKYIHPDILPD 227
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. TrEMBL
Match: A0A2R5L3V9 (Putative phosphatidylinositol transfer protein pdr16 OS=Ornithodoros turicata OX=34597 PE=4 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 1.868e-53
Identity = 89/187 (47.59%), Postives = 124/187 (66.31%), Query Frame = 2 
Query:  209 TRFIRAFGSASIAYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQ-DQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLPS 766
            TRF+RAF +   A+Q I+K   WR E+GV  +  +  DI+  +  K  ++L + D   RPVVYI    H VQ+R+ID +T+F+VY++E AC+KC E   D LCIIFDL  F + +MDY  ++ LI L S +YPERLG+CL+INSP IF GCW V++ WL D T +K++F NS E++  Y  P  LP+
Sbjct:   41 TRFLRAFRTIDNAFQAILKCNKWRSEYGVKKISPDQPDIKKNLESKKAMVLGNRDFCGRPVVYIPACKHNVQDRDIDELTRFIVYILEEACKKCFEEVIDNLCIIFDLKDFGLNSMDYPLIKNLIWLLSKHYPERLGVCLVINSPSIFSGCWVVIRGWLDDVTARKVVFINSQEELVKYIHPDILPN 227
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. TrEMBL
Match: A0A1B6KED9 (CRAL-TRIO domain-containing protein OS=Graphocephala atropunctata OX=36148 GN=g.29957 PE=4 SV=1)

HSP 1 Score: 182.956 bits (463), Expect = 2.401e-53
Identity = 88/187 (47.06%), Postives = 121/187 (64.71%), Query Frame = 2 
Query:  212 RFIRAFGSASIAYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQ-DQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLPSN 769
            R++RAF +   A+Q+I+K   WR E+GV  L+ N   ++   +KK   +L H D   RPV+YI  K+H   +R+ID +TKF+VY +E AC+KC E   D LCI+FDLN F +  MDY  V+ LI L S +YPERLG+CLIINSP +F GCW V+K WL ++T  K+ F +S E +  Y  P  LP +
Sbjct:   40 RYLRAFKTTDAAFQQILKTNKWRVEYGVAELNENHPIVKKHFDKKKARVLKHRDMQGRPVIYIPAKHHNANDRDIDELTKFIVYCLEEACKKCFEEVVDNLCIVFDLNDFGLSCMDYQVVKNLIWLLSRHYPERLGVCLIINSPTLFTGCWAVIKGWLDENTAGKVTFVSSEEDLCQYLIPDILPDD 226
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. TrEMBL
Match: A0A131XBH1 (Putative phosphatidylinositol transfer protein pdr16 OS=Hyalomma excavatum OX=257692 PE=2 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 9.772e-53
Identity = 89/186 (47.85%), Postives = 124/186 (66.67%), Query Frame = 2 
Query:  212 RFIRAFGSASIAYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQ-DQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLPS 766
            RF+RAF +   A+Q I+K   WR E+GV ++  ND DI+  I  K  ++LP+ D   RPV+YI    H VQ R ID +T+F+VY++E AC+KC E   D LCIIFDL  F + +MDY  ++ LI L S +YPERLGICLI+NSP IF GCW+V++ WL++ T +K++F  S + +  Y  P  LP+
Sbjct:   42 RFLRAFRTVDAAFQAILKCNKWRTEYGVKSISANDPDIKRNIEAKKAMVLPNRDFYGRPVIYIPACKHNVQEREIDELTRFIVYILEEACKKCFEEVVDNLCIIFDLKDFGLNSMDYPLIKNLIWLLSKHYPERLGICLILNSPAIFSGCWSVIRGWLNEVTAKKVVFIGSQDDLVKYVHPDILPN 227
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. TrEMBL
Match: L7M5M6 (Putative phosphatidylinositol transfer protein pdr16 OS=Rhipicephalus pulchellus OX=72859 PE=2 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 9.986e-53
Identity = 89/186 (47.85%), Postives = 124/186 (66.67%), Query Frame = 2 
Query:  212 RFIRAFGSASIAYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQ-DQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLPS 766
            RF+RAF +   A+Q I+K   WR E+GV ++  ND DI+  I  K  ++LP+ D   RPV+YI    H VQ R ID +T+F+VY++E AC+KC E   D LCIIFDL  F + +MDY  ++ LI L S +YPERLGICLI+NSP IF GCW+V++ WL++ T +K++F  S + +  Y  P  LP+
Sbjct:   42 RFLRAFRTVDAAFQAILKCNKWRTEYGVKSISANDPDIKRNIEAKKAMVLPNRDFYGRPVIYIPACKHNVQEREIDELTRFIVYILEEACKKCFEEVVDNLCIIFDLKDFGLNSMDYPLIKNLIWLLSKHYPERLGICLILNSPTIFSGCWSVIRGWLNEVTAKKVVFIGSQDDLVKYVHPDILPN 227
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Cavefish
Match: mospd2 (motile sperm domain containing 2 [Source:NCBI gene;Acc:103022972])

HSP 1 Score: 77.0258 bits (188), Expect = 1.378e-15
Identity = 47/173 (27.17%), Postives = 84/173 (48.55%), Query Frame = 2 
Query:  245 AYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLP 763
            A + I +   WRKEF +++L  + I   +   +   + L   DK    + +  VK H+   + I    +++ + +ER  ++  E    L ++FD++   IGN+D  FV+ +I  F  +YP+ L   ++   P I    W +VK WL    + K+ F  S   +  + D +HLP
Sbjct:   59 ALKMIDESFQWRKEFNLNDLTESSIPRWM--FETGAVYLHGYDKEGNKLFWFKVKLHVKDAKTIHDKKRYVAFWLERYAKR--EPGMPLTVVFDMSESGIGNIDMEFVKYIISCFKVFYPKFLSKMIMYEMPWIMNAAWKIVKTWLGPEAISKLKFV-SKSDVQTFIDAEHLP 226
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Cavefish
Match: mospd2 (motile sperm domain containing 2 [Source:NCBI gene;Acc:103022972])

HSP 1 Score: 76.6406 bits (187), Expect = 2.366e-15
Identity = 47/173 (27.17%), Postives = 84/173 (48.55%), Query Frame = 2 
Query:  245 AYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLP 763
            A + I +   WRKEF +++L  + I   +   +   + L   DK    + +  VK H+   + I    +++ + +ER  ++  E    L ++FD++   IGN+D  FV+ +I  F  +YP+ L   ++   P I    W +VK WL    + K+ F  S   +  + D +HLP
Sbjct:   65 ALKMIDESFQWRKEFNLNDLTESSIPRWM--FETGAVYLHGYDKEGNKLFWFKVKLHVKDAKTIHDKKRYVAFWLERYAKR--EPGMPLTVVFDMSESGIGNIDMEFVKYIISCFKVFYPKFLSKMIMYEMPWIMNAAWKIVKTWLGPEAISKLKFV-SKSDVQTFIDAEHLP 232
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Cavefish
Match: mospd2 (motile sperm domain containing 2 [Source:NCBI gene;Acc:103022972])

HSP 1 Score: 76.6406 bits (187), Expect = 2.387e-15
Identity = 47/173 (27.17%), Postives = 84/173 (48.55%), Query Frame = 2 
Query:  245 AYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLP 763
            A + I +   WRKEF +++L  + I   +   +   + L   DK    + +  VK H+   + I    +++ + +ER  ++  E    L ++FD++   IGN+D  FV+ +I  F  +YP+ L   ++   P I    W +VK WL    + K+ F  S   +  + D +HLP
Sbjct:   89 ALKMIDESFQWRKEFNLNDLTESSIPRWM--FETGAVYLHGYDKEGNKLFWFKVKLHVKDAKTIHDKKRYVAFWLERYAKR--EPGMPLTVVFDMSESGIGNIDMEFVKYIISCFKVFYPKFLSKMIMYEMPWIMNAAWKIVKTWLGPEAISKLKFV-SKSDVQTFIDAEHLP 256
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Cavefish
Match: ptpn9b (protein tyrosine phosphatase non-receptor type 9b [Source:ZFIN;Acc:ZDB-GENE-090313-52])

HSP 1 Score: 55.4546 bits (132), Expect = 2.437e-8
Identity = 30/136 (22.06%), Postives = 65/136 (47.79%), Query Frame = 2 
Query:  356 ILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLP 763
            +LP  D     +   + + H        +V + ++Y +++A E     +D L  I+D+ + +  N DY    ++++L    +P RL    I++SPL F+  + V++ ++ +   +++    +HE       P H+P
Sbjct:   54 VLPGRDAKGAALALFTARLHRPDVTTHKAVLQAIIYQLDKAIESIQTQRDGLIFIYDMTNSTYANFDYELCVKILNLLKGAFPARLKCVFIVSSPLWFRAPFAVLRLFVREKLRERVCTVKAHEL------PNHIP 183
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Yeast
Match: CSR1 (Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000004372])

HSP 1 Score: 87.8113 bits (216), Expect = 2.848e-20
Identity = 46/128 (35.94%), Postives = 71/128 (55.47%), Query Frame = 2 
Query:  383 RPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIEN-QDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLP 763
            RPV+ +  + H   ++    + KF + VIE++     EN      I+FDLN FS+ NMDYA V+ LI  F  +YPE LG  LI  +P IF   W ++K WL      KI+F  + +++  +  P+++P
Sbjct:  179 RPVILVRPRLHHSSDQTEQELEKFSLLVIEQSKLFFKENYPASTTILFDLNGFSMSNMDYAPVKFLITCFEAHYPESLGHLLIHKAPWIFNPIWNIIKNWLDPVVASKIVFTKNIDELHKFIQPQYIP 306
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Yeast
Match: SEC14 (Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs; SEC14 has a paralog, YKL091C, that arose from the whole genome duplication [Source:SGD;Acc:S000004684])

HSP 1 Score: 60.077 bits (144), Expect = 6.241e-11
Identity = 57/216 (26.39%), Postives = 93/216 (43.06%), Query Frame = 2 
Query:  182 INKVDVLTQTRFIRAFG-SASIAYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVLILPH-LDKLNRPVVY-------------ISVKNHIVQNR--NIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMD--YAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLPSNN 772
            I ++D  T  RF+RA      +A +       WRK++G D +  +    +  +  K      H  DK  RPV +             ++ +  +++N     +SV ++ +    RA    +E     C I DL   SI +     ++VR+  ++   YYPER+G   IIN+P  F   + + K +L   TV KI    S  Q  L    K +P+ N
Sbjct:   50 IERLDDSTLLRFLRARKFDVQLAKEMFENCEKWRKDYGTDTILQDFHYDEKPLIAKFYPQYYHKTDKDGRPVYFEELGAVNLHEMNKVTSEERMLKNLVWEYESVVQYRLPACSRAAGHLVETS---CTIMDLKGISISSAYSVMSYVREASYISQNYYPERMGKFYIINAPFGFSTAFRLFKPFLDPVTVSKIFILGSSYQKELL---KQIPAEN 259
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Yeast
Match: YKL091C (Putative phosphatidylinositol/phosphatidylcholine transfer protein; possibly involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study; YKL091C has a paralog, SEC14, that arose from the whole genome duplication [Source:SGD;Acc:S000001574])

HSP 1 Score: 55.0694 bits (131), Expect = 2.954e-9
Identity = 51/205 (24.88%), Postives = 92/205 (44.88%), Query Frame = 2 
Query:  188 KVDVLTQTRFIRAFG---SASIAYQRIVKYILWRKEFGVXXXXXXXXXXQIEINK---KCVLILP----HLDKLNRPVVY-------------ISVKNHIVQN--RNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDY--AFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNS 721
            ++D  T  RF+RA     +AS+  +  V+   WR+E+G + +  +  + +   +K   K   + P    H+DK  RP+ +             I+ +  +++N  +  +    + V    R     IE     C + DL   S+ N  +  ++++ +  +   YYPER+G   II+SP  F   + +VK +L   TV KI    S
Sbjct:   48 RLDDSTLLRFLRARKFDINASV--EMFVETERWREEYGANTIIEDYENNKEAEDKERIKLAKMYPQYYHHVDKDGRPLYFEELGGINLKKMYKITTEKQMLRNLVKEYELFATYRVPACSRRAGYLIETS---CTVLDLKGISLSNAYHVLSYIKDVADISQNYYPERMGKFYIIHSPFGFSTMFKMVKPFLDPVTVSKIFILGS 247
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Yeast
Match: PDR16 (Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000005175])

HSP 1 Score: 52.7582 bits (125), Expect = 1.803e-8
Identity = 42/174 (24.14%), Postives = 81/174 (46.55%), Query Frame = 2 
Query:  254 RIVKYILWRKEFGVXXXXXXXX--------XXQIEINKKCVLILPHLDKLNRPVVYISV--KNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFS-----IGNMDYAFV---RQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVK---RWLSDHTVQKIIF 712
            RI   + WR+EFG+ +L              ++ E  K+ +L     +   RP++Y+    +N    +R +    + +V+++ER  +     QD L ++ D   +       GN     +   ++++H+  T+YPERLG  L+ N P +    WT +K    ++   T +K++F
Sbjct:  107 RITMTLAWRREFGISHLGEEHGDKITADLVAVENESGKQVIL---GYENDARPILYLKPGRQNTKTSHRQV----QHLVFMLERVIDFMPAGQDSLALLIDFKDYPDVPKVPGNSKIPPIGVGKEVLHILQTHYPERLGKALLTNIPWL---AWTFLKLIHPFIDPLTREKLVF 270
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Yeast
Match: PDR17 (Phosphatidylinositol transfer protein (PITP); downregulates Plb1p-mediated turnover of phosphatidylcholine; forms a complex with Psd2p which appears essential for maintenance of vacuolar PE levels; found in the cytosol and microsomes; homologous to Pdr16p; deletion affects phospholipid composition [Source:SGD;Acc:S000005208])

HSP 1 Score: 46.595 bits (109), Expect = 2.198e-6
Identity = 41/186 (22.04%), Postives = 82/186 (44.09%), Query Frame = 2 
Query:  212 RFIRAFG-SASIAYQRIVKYILWRKEFGVX-------XXXXXXXXXQIEINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNM------DYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHE 727
            R++RA   + + A + + K ++WR+E G+         L  + + ++ E  K+ +L     D   RP+ Y+  KN      +     + +VY++E A     +  +++ ++ D  S+    +        +  R  +++   +YPERL  C++IN P        ++  +L   T  K IF    E
Sbjct:   97 RYLRANKWNTANAIKGLTKTLVWRREIGLTHGKEDKDPLTADKVAVENETGKQVIL---GFDNAKRPLYYM--KNGRQNTESSFRQVQELVYMMETATTVAPQGVEKITVLVDFKSYKEPGIITDKAPPISIARMCLNVMQDHYPERLAKCVLINIPWFAWAFLKMMYPFLDPATKAKAIFDEPFE 277
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Nematostella
Match: EDO33346 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSI7])

HSP 1 Score: 109.768 bits (273), Expect = 5.299e-30
Identity = 49/102 (48.04%), Postives = 68/102 (66.67%), Query Frame = 2 
Query:  470 ERACEKCIENQ-DQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLPSNN 772
            E A  KC E+  D +CI FD+  FS  NMDY FV+QLI L S  YPERLG+CL+IN+PLIF GCW++++ W++D T  KI+F    + +S +     LP ++
Sbjct:   35 EMALRKCNEDIIDNVCIAFDMKGFSFSNMDYGFVKQLIWLLSRRYPERLGVCLLINAPLIFSGCWSLIRLWINDVTANKIVFIRDRDHLSEFIATGILPIDD 136
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Nematostella
Match: EDO44504 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVS8])

HSP 1 Score: 74.3294 bits (181), Expect = 4.499e-15
Identity = 52/187 (27.81%), Postives = 92/187 (49.20%), Query Frame = 2 
Query:  215 FIRAFG-SASIAYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKK---CVLILPH-LDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHL 760
            FIRA       A + +V  + WRK FG++++       +  +N+K      + PH +DK    +V    +NH    +    + +F+V+++E+   + +    ++ ++FD+    + NMD  FV+ +   F+ YYP  L   L+I  P I    W +VK WLS + V+ I F  S + +  Y D + L
Sbjct:   48 FIRARSQDVDKALEALVFCLEWRKTFGLNDIT------EDNLNRKLFESGFLFPHNIDKDGNTIVLFVGRNHKKDPQQHHEMKRFLVFLLEK--HRKMYPSRKINLLFDMQETGLANMDMEFVKFITTCFTNYYPNTLSWLLVIELPWILTAAWKIVKTWLSPNAVRIIKFV-SKDALQEYIDREQL 225
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Nematostella
Match: EDO45692 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RS70])

HSP 1 Score: 47.7506 bits (112), Expect = 3.228e-6
Identity = 23/94 (24.47%), Postives = 50/94 (53.19%), Query Frame = 2 
Query:  425 NRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKI 706
            +R  + V   MV+ +E+A +  +  +  L +++D+++ S  N D+   ++++ L    YP RL    II+ PL F+    ++  +L +   ++I
Sbjct:  121 SRPSNKVLTGMVFQLEQAIKSPLTQRSGLVLLYDMSNSSYKNFDFDLSQKILELLKGGYPARLKSVFIISPPLWFKAVLLILSSFLREKIRERI 214
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Medaka
Match: mospd2 (motile sperm domain containing 2 [Source:NCBI gene;Acc:101169399])

HSP 1 Score: 84.7297 bits (208), Expect = 1.054e-18
Identity = 52/173 (30.06%), Postives = 88/173 (50.87%), Query Frame = 2 
Query:  245 AYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLP 763
            A + I   + WRKEFGV++L  + I   +   +   + L   DK    + +  VK HI   +      K++ + +ER  +K  E    L ++FD+    I N+D  FV+ +++ F  YYP+ L   +I++ P I    W +VK WL    + K+ F +  E ++ Y DP++LP
Sbjct:   64 ALKMIDDSLQWRKEFGVNDLSESTIPRWM--FETGAVFLHGYDKEGNKLFWFKVKLHIKDAKTAMDKKKYVAFWLERYAKK--EPGMPLTVVFDMAESGISNIDMDFVKYVVNCFKVYYPKFLSKMVIVDMPWIMNAAWKIVKSWLGPEAISKLKFASKAEVLN-YIDPEYLP 231
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Medaka
Match: mospd2 (motile sperm domain containing 2 [Source:NCBI gene;Acc:101169399])

HSP 1 Score: 83.9593 bits (206), Expect = 7.266e-18
Identity = 52/173 (30.06%), Postives = 88/173 (50.87%), Query Frame = 2 
Query:  245 AYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLP 763
            A + I   + WRKEFGV++L  + I   +   +   + L   DK    + +  VK HI   +      K++ + +ER  +K  E    L ++FD+    I N+D  FV+ +++ F  YYP+ L   +I++ P I    W +VK WL    + K+ F +  E ++ Y DP++LP
Sbjct:   64 ALKMIDDSLQWRKEFGVNDLSESTIPRWM--FETGAVFLHGYDKEGNKLFWFKVKLHIKDAKTAMDKKKYVAFWLERYAKK--EPGMPLTVVFDMAESGISNIDMDFVKYVVNCFKVYYPKFLSKMVIVDMPWIMNAAWKIVKSWLGPEAISKLKFASKAEVLN-YIDPEYLP 231
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Medaka
Match: ptpn9b (tyrosine-protein phosphatase non-receptor type 9 [Source:NCBI gene;Acc:101164280])

HSP 1 Score: 58.5362 bits (140), Expect = 2.387e-9
Identity = 30/127 (23.62%), Postives = 64/127 (50.39%), Query Frame = 2 
Query:  356 ILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQIS 736
            +LP  D     +   + + H        +V + ++Y +++A E     +D L  I+D+ + + GN DY    ++++L    +P RL    I++SPL F+  + V++ ++ +   +++    +HE IS
Sbjct:   81 VLPGRDAKGAALALFTARLHRPDVTTHKAVLQAIIYQLDKAIESVQTQRDGLIFIYDMTNSTYGNFDYELCVKILNLLKGAFPARLKCVFIVSSPLWFRAPFAVLRLFVREKLRERVCTVKAHELIS 207
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Medaka
Match: ptpn9b (tyrosine-protein phosphatase non-receptor type 9 [Source:NCBI gene;Acc:101164280])

HSP 1 Score: 58.5362 bits (140), Expect = 2.446e-9
Identity = 30/127 (23.62%), Postives = 64/127 (50.39%), Query Frame = 2 
Query:  356 ILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQIS 736
            +LP  D     +   + + H        +V + ++Y +++A E     +D L  I+D+ + + GN DY    ++++L    +P RL    I++SPL F+  + V++ ++ +   +++    +HE IS
Sbjct:   88 VLPGRDAKGAALALFTARLHRPDVTTHKAVLQAIIYQLDKAIESVQTQRDGLIFIYDMTNSTYGNFDYELCVKILNLLKGAFPARLKCVFIVSSPLWFRAPFAVLRLFVREKLRERVCTVKAHELIS 214
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Medaka
Match: ptpn9b (tyrosine-protein phosphatase non-receptor type 9 [Source:NCBI gene;Acc:101164280])

HSP 1 Score: 58.5362 bits (140), Expect = 2.998e-9
Identity = 30/127 (23.62%), Postives = 64/127 (50.39%), Query Frame = 2 
Query:  356 ILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQIS 736
            +LP  D     +   + + H        +V + ++Y +++A E     +D L  I+D+ + + GN DY    ++++L    +P RL    I++SPL F+  + V++ ++ +   +++    +HE IS
Sbjct:   88 VLPGRDAKGAALALFTARLHRPDVTTHKAVLQAIIYQLDKAIESVQTQRDGLIFIYDMTNSTYGNFDYELCVKILNLLKGAFPARLKCVFIVSSPLWFRAPFAVLRLFVREKLRERVCTVKAHELIS 214
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Planmine SMEST
Match: SMESG000081079.1 (SMESG000081079.1)

HSP 1 Score: 400.593 bits (1028), Expect = 9.529e-143
Identity = 221/221 (100.00%), Postives = 221/221 (100.00%), Query Frame = 2 
Query:  110 MTTENDXXXXXXXXXXXXXKTREDINKVDVLTQTRFIRAFGSASIAYQRIVKYILWRKEFGVXXXXXXXXXXQIEINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLPSNN 772
            MTTENDFEFQNLQLLLQENKTREDINKVDVLTQTRFIRAFGSASIAYQRIVKYILWRKEFGVDNLDLNDIDIQIEINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLPSNN
Sbjct:    1 MTTENDFEFQNLQLLLQENKTREDINKVDVLTQTRFIRAFGSASIAYQRIVKYILWRKEFGVDNLDLNDIDIQIEINKKCVLILPHLDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKIIFCNSHEQISLYFDPKHLPSNN 221
[Close]
BLAST of CRAL-TRIO domain-containing protein vs. Planmine SMEST
Match: SMESG000019717.1 (SMESG000019717.1)

HSP 1 Score: 59.3066 bits (142), Expect = 6.462e-10
Identity = 30/113 (26.55%), Postives = 54/113 (47.79%), Query Frame = 2 
Query:  368 LDKLNRPVVYISVKNHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDYAFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKI 706
             D+  RPV+Y S      +  +++     ++Y+IE A +           I D +  +I   +     ++  + S YYPERLG+   +N   +FQG W  +K +L  +T +K+
Sbjct:   87 FDESGRPVIYSSFSQANCRKNSVEESIAHVIYLIENATKTMKVGISNFVFIIDCSGVTIQACNPKLGYEVTQILSNYYPERLGVVFCVNHSQLFQGIWKAIKIFLHPNTSKKV 199
The following BLAST results are available for this feature:
BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
MOSPD21.462e-1229.52motile sperm domain containing 2 [Source:HGNC Symb... [more]
MOSPD22.111e-1229.52motile sperm domain containing 2 [Source:HGNC Symb... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
T03F7.71.242e-624.64pep chromosome:WBcel235:V:11311456:11313445:1 gene... [more]
T03F7.71.616e-624.64pep chromosome:WBcel235:V:11311456:11313445:1 gene... [more]
hpo-288.628e-623.49Major sperm protein [Source:UniProtKB/TrEMBL;Acc:... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG324859.055e-4543.32gene:FBgn0052485 transcript:FBtr0072985[more]
CG324071.774e-1124.24gene:FBgn0052407 transcript:FBtr0330118[more]
CG324071.774e-1124.24gene:FBgn0052407 transcript:FBtr0077032[more]
retm3.836e-1023.88gene:FBgn0031814 transcript:FBtr0329951[more]
retm4.451e-1023.88gene:FBgn0031814 transcript:FBtr0079272[more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
mospd21.537e-1428.24motile sperm domain containing 2 [Source:ZFIN;Acc:... [more]
mospd21.671e-1428.24motile sperm domain containing 2 [Source:ZFIN;Acc:... [more]
ptpn9b1.197e-822.46protein tyrosine phosphatase non-receptor type 9b ... [more]
sec14l84.402e-625.00SEC14-like lipid binding 8 [Source:ZFIN;Acc:ZDB-GE... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mospd21.347e-1325.50motile sperm domain containing 2 [Source:NCBI gene... [more]
mospd21.755e-1325.50motile sperm domain containing 2 [Source:NCBI gene... [more]
mospd24.617e-1225.50motile sperm domain containing 2 [Source:NCBI gene... [more]
mfhas14.486e-820.86malignant fibrous histiocytoma amplified sequence ... [more]
mfhas14.550e-820.86malignant fibrous histiocytoma amplified sequence ... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Mospd25.767e-1328.92motile sperm domain containing 2 [Source:MGI Symbo... [more]
Mospd27.047e-1328.92motile sperm domain containing 2 [Source:MGI Symbo... [more]
Mospd28.040e-1328.92motile sperm domain containing 2 [Source:MGI Symbo... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q10138|YAS2_SCHPO7.103e-2234.62CRAL-TRIO domain-containing protein C3H8.02 OS=Sch... [more]
sp|Q9HDZ5|YKP9_SCHPO2.870e-2027.08CRAL-TRIO domain-containing protein C589.09, mitoc... [more]
sp|Q06705|CSR1_YEAST1.978e-1835.94Phosphatidylinositol transfer protein CSR1 OS=Sacc... [more]
sp|Q757H2|CSR1_ASHGO5.837e-1831.52Phosphatidylinositol transfer protein CSR1 OS=Ashb... [more]
sp|Q55CU8|PITC_DICDI5.374e-1326.86Phosphatidylinositol transfer protein 3 OS=Dictyos... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A293LCF12.813e-5447.59CRAL-TRIO domain-containing protein (Fragment) OS=... [more]
A0A2R5L3V91.868e-5347.59Putative phosphatidylinositol transfer protein pdr... [more]
A0A1B6KED92.401e-5347.06CRAL-TRIO domain-containing protein OS=Graphocepha... [more]
A0A131XBH19.772e-5347.85Putative phosphatidylinositol transfer protein pdr... [more]
L7M5M69.986e-5347.85Putative phosphatidylinositol transfer protein pdr... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
mospd21.378e-1527.17motile sperm domain containing 2 [Source:NCBI gene... [more]
mospd22.366e-1527.17motile sperm domain containing 2 [Source:NCBI gene... [more]
mospd22.387e-1527.17motile sperm domain containing 2 [Source:NCBI gene... [more]
ptpn9b2.437e-822.06protein tyrosine phosphatase non-receptor type 9b ... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
CSR12.848e-2035.94Phosphatidylinositol transfer protein; has a poten... [more]
SEC146.241e-1126.39Phosphatidylinositol/phosphatidylcholine transfer ... [more]
YKL091C2.954e-924.88Putative phosphatidylinositol/phosphatidylcholine ... [more]
PDR161.803e-824.14Phosphatidylinositol transfer protein (PITP); cont... [more]
PDR172.198e-622.04Phosphatidylinositol transfer protein (PITP); down... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO333465.299e-3048.04Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO445044.499e-1527.81Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO456923.228e-624.47Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mospd21.054e-1830.06motile sperm domain containing 2 [Source:NCBI gene... [more]
mospd27.266e-1830.06motile sperm domain containing 2 [Source:NCBI gene... [more]
ptpn9b2.387e-923.62tyrosine-protein phosphatase non-receptor type 9 [... [more]
ptpn9b2.446e-923.62tyrosine-protein phosphatase non-receptor type 9 [... [more]
ptpn9b2.998e-923.62tyrosine-protein phosphatase non-receptor type 9 [... [more]
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BLAST of CRAL-TRIO domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000081079.19.529e-143100.00SMESG000081079.1[more]
SMESG000019717.16.462e-1026.55SMESG000019717.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011535 ID=SMED30011535|Name=CRAL-TRIO domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=867bp
CCTAGATCAAAGTTTAAAAAGAGTTATGTGTTTTGGATAAATTGATCTAC
AGCTTAAATCATTTACATATTTTAATTAATTTTATTAATCTTTCAAACAT
CATTGAATAATGACAACAGAAAATGATTTTGAATTTCAAAATTTGCAATT
ATTATTACAAGAAAACAAAACTAGAGAAGATATTAATAAAGTTGACGTTT
TAACACAAACTCGCTTTATTCGTGCTTTTGGATCCGCTTCTATTGCATAT
CAAAGAATTGTTAAATATATTTTATGGCGAAAAGAATTCGGAGTTGATAA
TTTGGATTTAAATGACATTGATATTCAAATTGAAATAAATAAAAAATGCG
TCCTTATACTTCCTCATTTAGATAAGTTAAATAGACCTGTCGTTTATATC
TCTGTCAAAAATCATATTGTTCAAAACCGAAACATTGATAGTGTCACCAA
ATTTATGGTTTATGTAATTGAAAGAGCTTGTGAAAAATGTATAGAAAATC
AAGATCAATTATGTATAATATTTGATTTAAATTCATTTTCTATCGGAAAT
ATGGACTACGCATTTGTTCGACAATTAATTCATTTGTTTTCTACTTATTA
TCCAGAGCGTTTAGGTATTTGTCTCATCATTAATTCTCCGTTAATATTCC
AAGGTTGTTGGACAGTGGTAAAAAGATGGTTATCAGATCACACTGTGCAG
AAGATCATATTCTGCAATTCACACGAACAAATTAGTCTCTATTTTGATCC
AAAACATTTGCCTTCAAATAACTGAGTTATGTATTTATAATTTTAAAATT
TATCTTCAGAGAGATATTTTTAACTATTGTCTTATTATGGCCAAGATTAT
TTATTTTATTGTATAGC
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protein sequence of SMED30011535-orf-1

>SMED30011535-orf-1 ID=SMED30011535-orf-1|Name=SMED30011535-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=222bp
MTTENDFEFQNLQLLLQENKTREDINKVDVLTQTRFIRAFGSASIAYQRI
VKYILWRKEFGVDNLDLNDIDIQIEINKKCVLILPHLDKLNRPVVYISVK
NHIVQNRNIDSVTKFMVYVIERACEKCIENQDQLCIIFDLNSFSIGNMDY
AFVRQLIHLFSTYYPERLGICLIINSPLIFQGCWTVVKRWLSDHTVQKII
FCNSHEQISLYFDPKHLPSNN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000016pharynx
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR036865CRAL-TRIO_dom_sf
IPR036273CRAL/TRIO_N_dom_sf
IPR001251CRAL-TRIO_dom
IPR011074CRAL/TRIO_N_dom
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
Vocabulary: molecular function
TermDefinition
GO:0008526phosphatidylinositol transporter activity
Vocabulary: biological process
TermDefinition
GO:0015914phospholipid transport
GO:0071852fungal-type cell wall organization or biogenesis
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001251CRAL-TRIO lipid binding domainSMARTSM00516sec14_4coord: 73..221
e-value: 1.5E-24
score: 97.6
IPR001251CRAL-TRIO lipid binding domainPFAMPF00650CRAL_TRIOcoord: 75..219
e-value: 6.8E-34
score: 116.9
IPR001251CRAL-TRIO lipid binding domainPROSITEPS50191CRAL_TRIOcoord: 83..221
score: 16.714
IPR001251CRAL-TRIO lipid binding domainCDDcd00170SEC14coord: 73..220
e-value: 5.36907E-30
score: 107.086
IPR036865CRAL-TRIO lipid binding domain superfamilyGENE3DG3DSA:3.40.525.10coord: 2..220
e-value: 2.3E-43
score: 150.4
IPR036865CRAL-TRIO lipid binding domain superfamilySUPERFAMILYSSF52087CRAL/TRIO domaincoord: 75..219
NoneNo IPR availablePANTHERPTHR46590FAMILY NOT NAMEDcoord: 35..220
IPR036273CRAL/TRIO, N-terminal domain superfamilySUPERFAMILYSSF46938CRAL/TRIO N-terminal domaincoord: 10..68