Mediator of RNA polymerase II transcription subunit 21

Overview
NameMediator of RNA polymerase II transcription subunit 21
Smed IDSMED30011519
Length (bp)1448
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Mediator of RNA polymerase II transcription subunit 21 (SMED30011519) t-SNE clustered cells

Violin plots show distribution of expression levels for Mediator of RNA polymerase II transcription subunit 21 (SMED30011519) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Mediator of RNA polymerase II transcription subunit 21 (SMED30011519) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Mediator of RNA polymerase II transcription subunit 21 (SMED30011519) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30011519

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30011519h1SMcG0014030 Contig2568GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
dorsal ventral margin of the whole animalSMED30011519h1SMcG0014030 Contig2568GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30011519h1SMcG0014030 Contig2568GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30011519h1SMcG0014030 dd_Smed_v4_2670_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30011519h1SMcG0014030 dd_Smed_v4_2670_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30011519h1SMcG0014030 dd_Smed_v4_2670_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30011519h1SMcG0014030 dd_Smed_v4_2670_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30011519h1SMcG0014030 dd_Smed_v4_2670_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Human
Match: MED21 (mediator complex subunit 21 [Source:HGNC Symbol;Acc:HGNC:11473])

HSP 1 Score: 64.3142 bits (155), Expect = 8.651e-12
Identity = 41/139 (29.50%), Postives = 69/139 (49.64%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQL 462
            ADRL++LQD +N  A   C++IGV+QQ   P+ F+         +  NP          + +Y + FA +I  T   I+ LID LP E+ +  +  ++L  L  +  ++   L   V   +  LE ++  L +I++ QL
Sbjct:    2 ADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFNNIQTAINKDQPANP----------TEEYAQLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATCLEDVVYRGDMLLEKIQSALADIAQSQL 130
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Human
Match: MED21 (mediator complex subunit 21 [Source:HGNC Symbol;Acc:HGNC:11473])

HSP 1 Score: 64.6994 bits (156), Expect = 8.845e-12
Identity = 42/150 (28.00%), Postives = 73/150 (48.67%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQLTAEALEAEILK 495
            ADRL++LQD +N  A   C++IGV+QQ   P+ F+         +  NP          + +Y + FA +I  T   I+ LID LP E+ +  +  ++L  L  +  ++   L   V   +  LE ++  L +I++ QL   + E  + +
Sbjct:    2 ADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFNNIQTAINKDQPANP----------TEEYAQLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATCLEDVVYRGDMLLEKIQSALADIAQSQLKTRSGEVSVTQ 141
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Human
Match: GET1 (guided entry of tail-anchored proteins factor 1 [Source:HGNC Symbol;Acc:HGNC:12790])

HSP 1 Score: 53.9138 bits (128), Expect = 6.373e-8
Identity = 25/91 (27.47%), Postives = 56/91 (61.54%), Query Frame = 2 
Query:  599 FLSKIGCMLMNRSKADNDRISKLKELTNEKSKLNMTDNFAKYSKLERQIKKMESELQTNSQVRCKNSLTVRLVISMSLKILQVLTFMTIFY 871
            F+S++   L   ++ ++   ++++++  E S +NM D FA+Y++LER+I KM  +L+T+ + R      ++ VIS++  +LQ    +++ +
Sbjct:   34 FMSRV---LQKDAEQESQMRAEIQDMKQELSTVNMMDEFARYARLERKINKMTDKLKTHVKARTAQLAKIKWVISVAFYVLQAALMISLIW 121
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Human
Match: WRB-SH3BGR (WRB-SH3BGR readthrough [Source:NCBI gene;Acc:106865373])

HSP 1 Score: 53.9138 bits (128), Expect = 7.296e-8
Identity = 25/91 (27.47%), Postives = 56/91 (61.54%), Query Frame = 2 
Query:  599 FLSKIGCMLMNRSKADNDRISKLKELTNEKSKLNMTDNFAKYSKLERQIKKMESELQTNSQVRCKNSLTVRLVISMSLKILQVLTFMTIFY 871
            F+S++   L   ++ ++   ++++++  E S +NM D FA+Y++LER+I KM  +L+T+ + R      ++ VIS++  +LQ    +++ +
Sbjct:   34 FMSRV---LQKDAEQESQMRAEIQDMKQELSTVNMMDEFARYARLERKINKMTDKLKTHVKARTAQLAKIKWVISVAFYVLQAALMISLIW 121
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Human
Match: GET1 (guided entry of tail-anchored proteins factor 1 [Source:HGNC Symbol;Acc:HGNC:12790])

HSP 1 Score: 52.373 bits (124), Expect = 1.090e-7
Identity = 22/71 (30.99%), Postives = 46/71 (64.79%), Query Frame = 2 
Query:  659 SKLKELTNEKSKLNMTDNFAKYSKLERQIKKMESELQTNSQVRCKNSLTVRLVISMSLKILQVLTFMTIFY 871
            ++++++  E S +NM D FA+Y++LER+I KM  +L+T+ + R      ++ VIS++  +LQ    +++ +
Sbjct:   17 AEIQDMKQELSTVNMMDEFARYARLERKINKMTDKLKTHVKARTAQLAKIKWVISVAFYVLQAALMISLIW 87
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Celegans
Match: mdt-21 (Mediator of RNA polymerase II transcription subunit 21 [Source:UniProtKB/Swiss-Prot;Acc:Q6BER6])

HSP 1 Score: 50.0618 bits (118), Expect = 2.175e-7
Identity = 43/143 (30.07%), Postives = 68/143 (47.55%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGK--ELLKTVDYLEKKLEFVREKLIEISEVQLTA 468
            ADR+++LQD+IN+ A  + ++IGV+Q  A P  F     +              LED+ +   C  FA  I +    I  LID  P E  + E       ++ ND     K  EL+  V    + +  V++KL EIS+ Q+++
Sbjct:    2 ADRMTQLQDMINEMAGLMTNAIGVLQATAPPCEFGAISQE--------------LEDEPN---CAIFAAGIAKAAKNIEILIDSFPIEAGNQEAEVEE-KMIKNDEKQREKVNELVGLVGDSNRLVGVVQKKLAEISKTQMSS 126
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Fly
Match: MED21 (gene:FBgn0040020 transcript:FBtr0113739)

HSP 1 Score: 77.7962 bits (190), Expect = 6.980e-17
Identity = 50/139 (35.97%), Postives = 77/139 (55.40%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQL 462
            ADRL++LQD +NQQA++ C++IGVIQQ + PS F  F+     +    P            DY + FA++I      I+ LI+ LP ED S+E+ NS+L  L  +   + ++L + V   E  LE ++  L  I++ QL
Sbjct:    2 ADRLTQLQDTVNQQAEHFCNAIGVIQQTSLPSKFVNFERIGPQTPIPCP---------PQEDYAQLFAQLIARCAKDIDTLIESLPNEDSSIELQNSSLKRLEIENQGTARDLEEVVQKGELLLEKMQYSLECIAQAQL 131
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Fly
Match: MED21 (gene:FBgn0040020 transcript:FBtr0113740)

HSP 1 Score: 77.7962 bits (190), Expect = 6.980e-17
Identity = 50/139 (35.97%), Postives = 77/139 (55.40%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQL 462
            ADRL++LQD +NQQA++ C++IGVIQQ + PS F  F+     +    P            DY + FA++I      I+ LI+ LP ED S+E+ NS+L  L  +   + ++L + V   E  LE ++  L  I++ QL
Sbjct:    2 ADRLTQLQDTVNQQAEHFCNAIGVIQQTSLPSKFVNFERIGPQTPIPCP---------PQEDYAQLFAQLIARCAKDIDTLIESLPNEDSSIELQNSSLKRLEIENQGTARDLEEVVQKGELLLEKMQYSLECIAQAQL 131
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Zebrafish
Match: med21 (mediator complex subunit 21 [Source:NCBI gene;Acc:406732])

HSP 1 Score: 69.707 bits (169), Expect = 6.803e-14
Identity = 42/139 (30.22%), Postives = 71/139 (51.08%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQL 462
            ADRL++LQD +N  A   C++IGV+QQ A P+ FS         +  NP          + +Y + FA +I  T   ++ LID LP E+ +  +  ++L  L  +  ++   L + V   +  LE ++  L +I++ QL
Sbjct:    2 ADRLTQLQDAVNSLADQFCNAIGVLQQCAPPASFSNIQTAINKDQPSNP----------TEEYAQLFAALIARTAKDVDVLIDSLPSEESTAALQAASLRQLEEENQEAAARLEEVVYRGDALLEKIQTALADIAQSQL 130
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Xenopus
Match: med21 (mediator complex subunit 21 [Source:NCBI gene;Acc:100302398])

HSP 1 Score: 66.2402 bits (160), Expect = 2.009e-12
Identity = 43/139 (30.94%), Postives = 69/139 (49.64%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQL 462
            ADRL++LQD +N  A   C++IGV+QQ A P+ FS         +  NP          + +Y + FA +I  T   I  LID LP E+ +  +  ++L  L  +   +   L + V   +  LE ++  L +I++ QL
Sbjct:    2 ADRLTQLQDALNSLADQFCNAIGVLQQCAPPASFSNIQTGINKDQPPNP----------TEEYAQLFAALIARTAKDIEVLIDSLPSEESTAALQAASLYQLEEENHAAAARLEEVVYRGDLLLEKIQTALADIAQSQL 130
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Xenopus
Match: med21 (mediator complex subunit 21 [Source:NCBI gene;Acc:100302398])

HSP 1 Score: 50.447 bits (119), Expect = 6.590e-7
Identity = 36/127 (28.35%), Postives = 59/127 (46.46%), Query Frame = 1 
Query:   82 QQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQL 462
            Q A   C++IGV+QQ A P+ FS         +  NP          + +Y + FA +I  T   I  LID LP E+ +  +  ++L  L  +   +   L + V   +  LE ++  L +I++ QL
Sbjct:   26 QLADQFCNAIGVLQQCAPPASFSNIQTGINKDQPPNP----------TEEYAQLFAALIARTAKDIEVLIDSLPSEESTAALQAASLYQLEEENHAAAARLEEVVYRGDLLLEKIQTALADIAQSQL 142
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Xenopus
Match: nkain1 (Sodium/potassium transporting ATPase interacting 1cting 1 [Source:Xenbase;Acc:XB-GENE-946558])

HSP 1 Score: 49.6766 bits (117), Expect = 1.735e-6
Identity = 26/91 (28.57%), Postives = 52/91 (57.14%), Query Frame = 2 
Query:  599 FLSKIGCMLMNRSKADNDRISKLKELTNEKSKLNMTDNFAKYSKLERQIKKMESELQTNSQVRCKNSLTVRLVISMSLKILQVLTFMTIFY 871
            F S +  +L   ++ + +  S ++ +  E S ++M D FAKY++LER+I KM  +L+T+ + R      ++ VIS+   +LQ    +++ +
Sbjct:   35 FSSLVSRLLQEDAEQEYEMRSDIQTMRQELSNISMMDEFAKYARLERKINKMTDKLKTHVKSRTAQLSKIKWVISIVFYVLQAALMVSLIW 125
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Xenopus
Match: med21 (mediator complex subunit 21 [Source:NCBI gene;Acc:100302398])

HSP 1 Score: 49.6766 bits (117), Expect = 1.827e-6
Identity = 36/127 (28.35%), Postives = 59/127 (46.46%), Query Frame = 1 
Query:   82 QQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQL 462
            Q A   C++IGV+QQ A P+ FS         +  NP          + +Y + FA +I  T   I  LID LP E+ +  +  ++L  L  +   +   L + V   +  LE ++  L +I++ QL
Sbjct:   26 QLADQFCNAIGVLQQCAPPASFSNIQTGINKDQPPNP----------TEEYAQLFAALIARTAKDIEVLIDSLPSEESTAALQAASLYQLEEENHAAAARLEEVVYRGDLLLEKIQTALADIAQSQL 142
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Mouse
Match: Med21 (mediator complex subunit 21 [Source:MGI Symbol;Acc:MGI:1347064])

HSP 1 Score: 65.4698 bits (158), Expect = 2.327e-12
Identity = 42/139 (30.22%), Postives = 69/139 (49.64%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQL 462
            ADRL++LQD +N  A   C++IGV+QQ   P+ FS         +  NP          + +Y + FA +I  T   I+ LID LP E+ +  +  ++L  L  +  ++   L   V   +  LE ++  L +I++ QL
Sbjct:    2 ADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFSNIQTAINKDQPANP----------TEEYAQLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATCLEDVVYRGDMLLEKIQSALADIAQSQL 130
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Mouse
Match: Med21 (mediator complex subunit 21 [Source:MGI Symbol;Acc:MGI:1347064])

HSP 1 Score: 65.4698 bits (158), Expect = 2.327e-12
Identity = 42/139 (30.22%), Postives = 69/139 (49.64%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQL 462
            ADRL++LQD +N  A   C++IGV+QQ   P+ FS         +  NP          + +Y + FA +I  T   I+ LID LP E+ +  +  ++L  L  +  ++   L   V   +  LE ++  L +I++ QL
Sbjct:    2 ADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFSNIQTAINKDQPANP----------TEEYAQLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATCLEDVVYRGDMLLEKIQSALADIAQSQL 130
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Mouse
Match: Med21 (mediator complex subunit 21 [Source:MGI Symbol;Acc:MGI:1347064])

HSP 1 Score: 58.9214 bits (141), Expect = 1.315e-10
Identity = 32/89 (35.96%), Postives = 46/89 (51.69%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEED 312
            ADRL++LQD +N  A   C++IGV+QQ   P+ FS         +  NP          + +Y + FA +I  T   I+ LID LP E+
Sbjct:    2 ADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFSNIQTAINKDQPANP----------TEEYAQLFAALIARTAKDIDVLIDSLPSEE 80
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Mouse
Match: Wrb (tryptophan rich basic protein [Source:MGI Symbol;Acc:MGI:2136882])

HSP 1 Score: 51.2174 bits (121), Expect = 3.927e-7
Identity = 25/91 (27.47%), Postives = 54/91 (59.34%), Query Frame = 2 
Query:  599 FLSKIGCMLMNRSKADNDRISKLKELTNEKSKLNMTDNFAKYSKLERQIKKMESELQTNSQVRCKNSLTVRLVISMSLKILQVLTFMTIFY 871
            F+S++   L   ++ ++   ++++ +  E S +NM D FA+Y++LER+I KM  +L+T+ + R      ++  IS++  ILQ    +++ +
Sbjct:   34 FISRV---LQKDAEQESQMRAEIQGMKQELSTVNMMDEFARYARLERKINKMTDKLKTHVKARTAQLAKIKWFISVAFYILQAALMISLIW 121
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. UniProt/SwissProt
Match: sp|Q9W5P1|MED21_DROME (Mediator of RNA polymerase II transcription subunit 21 OS=Drosophila melanogaster OX=7227 GN=MED21 PE=1 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 6.997e-16
Identity = 50/139 (35.97%), Postives = 77/139 (55.40%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQL 462
            ADRL++LQD +NQQA++ C++IGVIQQ + PS F  F+     +    P            DY + FA++I      I+ LI+ LP ED S+E+ NS+L  L  +   + ++L + V   E  LE ++  L  I++ QL
Sbjct:    2 ADRLTQLQDTVNQQAEHFCNAIGVIQQTSLPSKFVNFERIGPQTPIPCP---------PQEDYAQLFAQLIARCAKDIDTLIESLPNEDSSIELQNSSLKRLEIENQGTARDLEEVVQKGELLLEKMQYSLECIAQAQL 131
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. UniProt/SwissProt
Match: sp|Q16RX1|MED21_AEDAE (Mediator of RNA polymerase II transcription subunit 21 OS=Aedes aegypti OX=7159 GN=MED21 PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 4.000e-15
Identity = 47/139 (33.81%), Postives = 77/139 (55.40%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQL 462
            ADRL++LQD +NQQA++ C+SIG++QQ + PS F  F+     + + N             DY + F+ +I      I+ LI+ LP E+ S+E+   AL  L  +  +S ++L + V   E  LE ++  L +I++ QL
Sbjct:    2 ADRLTQLQDTVNQQAEHFCNSIGILQQCSVPSKFPGFERTGSQTPQQN----------QQEDYAQLFSTLISRCAKDIDTLIESLPSEESSIELQVQALKRLEIENQESAEKLEEVVRKGELLLEKIQAALSDIAQSQL 130
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. UniProt/SwissProt
Match: sp|Q7ZTI5|MED21_DANRE (Mediator of RNA polymerase II transcription subunit 21 OS=Danio rerio OX=7955 GN=med21 PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 5.135e-13
Identity = 42/139 (30.22%), Postives = 71/139 (51.08%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQL 462
            ADRL++LQD +N  A   C++IGV+QQ A P+ FS         +  NP          + +Y + FA +I  T   ++ LID LP E+ +  +  ++L  L  +  ++   L + V   +  LE ++  L +I++ QL
Sbjct:    2 ADRLTQLQDAVNSLADQFCNAIGVLQQCAPPASFSNIQTAINKDQPSNP----------TEEYAQLFAALIARTAKDVDVLIDSLPSEESTAALQAASLRQLEEENQEAAARLEEVVYRGDALLEKIQTALADIAQSQL 130
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. UniProt/SwissProt
Match: sp|Q2TBU8|MED21_BOVIN (Mediator of RNA polymerase II transcription subunit 21 OS=Bos taurus OX=9913 GN=MED21 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 5.009e-12
Identity = 42/139 (30.22%), Postives = 69/139 (49.64%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQL 462
            ADRL++LQD +N  A   C++IGV+QQ   P+ FS         +  NP          + +Y + FA +I  T   I+ LID LP E+ +  +  ++L  L  +  ++   L   V   +  LE ++  L +I++ QL
Sbjct:    2 ADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFSNIQTAINKDQPANP----------TEEYAQLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATSLEDVVYRGDMLLEKIQSALADIAQSQL 130
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. UniProt/SwissProt
Match: sp|Q5RE46|MED21_PONAB (Mediator of RNA polymerase II transcription subunit 21 OS=Pongo abelii OX=9601 GN=MED21 PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.139e-11
Identity = 44/139 (31.65%), Postives = 71/139 (51.08%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQL 462
            ADRL++LQD +N  A   C++IGV+QQ   P+ FS       +  KD P         S+ +Y + FA +I  T   I+ LID LP E+ +  +  ++L  L  +  ++   L   V   +  LE ++  L +I++ QL
Sbjct:    2 ADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFSNIQT---AINKDQPA-------NSTEEYAQLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATCLEDVVYRGDMLLEKIQSALADIAQSQL 130
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. TrEMBL
Match: A0A5K4FD93 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 2.066e-33
Identity = 67/154 (43.51%), Postives = 98/154 (63.64%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQLTAEALEAEILKNYYG 507
            ADRL+ELQD IN QA+NLC++IGVIQQ AQPSFFS+F+W +RS++   P  Q  ++ + + D  + FA  I  T  Q++ LI  LPEE+ S +I  + +  L   Y   G +L +    LE +L  VR  L  I++ QLT ++LE+E+ + +YG
Sbjct:    2 ADRLTELQDAINLQAENLCNAIGVIQQVAQPSFFSDFNWASRSAK---PEYQAFIQAQPTDDIARSFAVAISATAKQLDALIGALPEEEASADIQRATVHKLLEAYRSEGAKLARITTKLESRLAEVRRCLTAIAQTQLTTQSLESEVYREFYG 152
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. TrEMBL
Match: A0A4Z2DJF8 (Mediator of RNA polymerase II transcription subunit 21 OS=Schistosoma japonicum OX=6182 GN=EWB00_000257 PE=3 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 3.721e-33
Identity = 67/154 (43.51%), Postives = 97/154 (62.99%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQLTAEALEAEILKNYYG 507
            ADRL+ELQD IN QA+NLC++IGVIQQ AQPSFFS+F+W +RS +   P  Q  ++ + + D  + FA  I  T  Q++ LI  LPEE+ S +I  + +  L   Y   G +L +    LE +L  VR  L  I++ QLT ++LE+E+ + +YG
Sbjct:    2 ADRLTELQDAINLQAENLCNAIGVIQQVAQPSFFSDFNWASRSVK---PEYQAFIQSQPTDDIARSFAVAISATAKQLDTLIGALPEEEASADIQRATVHKLLEGYRTEGAKLARITTKLESRLAEVRRCLTAIAQTQLTTQSLESEVYREFYG 152
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. TrEMBL
Match: Q5DD16 (Mediator of RNA polymerase II transcription subunit 21 OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 4.256e-33
Identity = 67/154 (43.51%), Postives = 97/154 (62.99%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQLTAEALEAEILKNYYG 507
            ADRL+ELQD IN QA+NLC++IGVIQQ AQPSFFS+F+W +RS +   P  Q  ++ + + D  + FA  I  T  Q++ LI  LPEE+ S +I  + +  L   Y   G +L +    LE +L  VR  L  I++ QLT ++LE+E+ + +YG
Sbjct:    2 ADRLTELQDAINLQAENLCNAIGVIQQVAQPSFFSDFNWASRSVK---PEYQAFIQSQPTDDIARSFAVAISATAKQLDTLIGALPEEEASADIQRATVHKLLEGYRAEGAKLARITTKLESRLAEVRRCLTAIAQTQLTTQSLESEVYREFYG 152
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. TrEMBL
Match: A0A4S2L4H9 (Mediator of RNA polymerase II transcription subunit 21 OS=Opisthorchis felineus OX=147828 GN=CRM22_009991 PE=3 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 2.050e-31
Identity = 70/147 (47.62%), Postives = 94/147 (63.95%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQLTAEALEAE 486
            ADRL+ELQD IN QA+NLC++IGVIQQ AQPSFFS+F+W +R+S+   P  Q  L+ +   D  + FA VI  T  Q++ LI  LPEE+ S EI  +A   L  DY   G +L +  + LE +L  VR  L  I++ QLT ++LE E
Sbjct:    2 ADRLTELQDAINLQAENLCNAIGVIQQVAQPSFFSDFNWSSRASK---PEYQAFLQGQPPDDIGRNFAVVIAATARQLDALIGSLPEEEASSEIQRAAFQHLVEDYRTEGWKLTRVTERLESRLTEVRLFLTAIAQTQLTTQSLETE 145
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. TrEMBL
Match: A0A4Z2DKA2 (Mediator of RNA polymerase II transcription subunit 21 OS=Schistosoma japonicum OX=6182 GN=EWB00_000257 PE=3 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 9.376e-31
Identity = 65/149 (43.62%), Postives = 94/149 (63.09%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQLTAEALEAEIL 492
            ADRL+ELQD IN QA+NLC++IGVIQQ AQPSFFS+F+W +RS +   P  Q  ++ + + D  + FA  I  T  Q++ LI  LPEE+ S +I  + +  L   Y   G +L +    LE +L  VR  L  I++ QLT ++LE+E++
Sbjct:    2 ADRLTELQDAINLQAENLCNAIGVIQQVAQPSFFSDFNWASRSVK---PEYQAFIQSQPTDDIARSFAVAISATAKQLDTLIGALPEEEASADIQRATVHKLLEGYRTEGAKLARITTKLESRLAEVRRCLTAIAQTQLTTQSLESEVI 147
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Cavefish
Match: med21 (mediator complex subunit 21 [Source:NCBI gene;Acc:103026743])

HSP 1 Score: 68.9366 bits (167), Expect = 1.157e-13
Identity = 42/139 (30.22%), Postives = 71/139 (51.08%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQL 462
            ADRL++LQD +N  A   C++IGV+QQ A P+ FS         +  NP          + +Y + FA +I  T   ++ LID LP E+ +  +  ++L  L  +  ++   L + V   +  LE ++  L +I++ QL
Sbjct:    2 ADRLTQLQDAVNSLADQFCNAIGVLQQCAPPASFSNIQTAINKDQPSNP----------TEEYAQLFAALIARTAKDVDVLIDSLPSEESTAALQAASLRQLEEENHEAAARLEEVVYRGDLLLEKIQSALADIAQSQL 130
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Sea Lamprey
Match: get1 (guided entry of tail-anchored proteins factor 1 [Source:NCBI gene;Acc:335756])

HSP 1 Score: 47.7506 bits (112), Expect = 8.590e-7
Identity = 21/67 (31.34%), Postives = 42/67 (62.69%), Query Frame = 2 
Query:  671 ELTNEKSKLNMTDNFAKYSKLERQIKKMESELQTNSQVRCKNSLTVRLVISMSLKILQVLTFMTIFY 871
            ++  E + +++ D FA+Y++LER+I K   EL+T+ Q R     T++ +I++S   LQ +  +T+ +
Sbjct:   56 KMRRELATISVMDEFARYARLERRINKASDELKTSLQARTTQLTTMKWIINISFYTLQAVVMITLIW 122
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Nematostella
Match: EDO47412 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RLY3])

HSP 1 Score: 82.0333 bits (201), Expect = 9.404e-19
Identity = 47/136 (34.56%), Postives = 74/136 (54.41%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISE 453
            ADR+++LQD +NQ A   C+S+G++QQ A+P  F  FD         NP         +   +   FA +I  T   I+YLID LP E+ S E+  ++L  L  +  ++G++L + V   EK L  +++ L EI+E
Sbjct:    2 ADRITQLQDALNQLADQFCNSVGILQQTAEPGQFPGFDKGTNPKLSSNP--------SNFEGHGALFATLIARTAKDIDYLIDSLPSEECSPELQAASLQRLEAENQEAGRKLEEVVRKGEKLLALIQQALNEIAE 129
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Medaka
Match: med21 (mediator complex subunit 21 [Source:NCBI gene;Acc:101163865])

HSP 1 Score: 70.8626 bits (172), Expect = 2.493e-14
Identity = 42/139 (30.22%), Postives = 72/139 (51.80%), Query Frame = 1 
Query:   46 ADRLSELQDIINQQAQNLCDSIGVIQQFAQPSFFSEFDWKARSSRKDNPIIQLVLEDKSSPDYCKQFAKVIRETFTQINYLIDGLPEEDLSLEIHNSALSLLHNDYIDSGKELLKTVDYLEKKLEFVREKLIEISEVQL 462
            ADRL++LQD +N  A   C++IGV+QQFA P+ FS         +  NP          + +Y + FA +I  T   ++ LID LP E+ +  +  ++L  L  +  ++   L + V   +  L+ ++  L +I++ QL
Sbjct:    2 ADRLTQLQDAVNSLADQFCNAIGVLQQFAPPASFSNIQTAINKDQPANP----------TEEYAQLFAALIARTAKDVDVLIDSLPSEESTAALQAASLRQLEEENHEAAARLEEVVFRGDMLLKRIQHALADIAQSQL 130
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Planmine SMEST
Match: SMESG000036235.1 (SMESG000036235.1)

HSP 1 Score: 322.013 bits (824), Expect = 2.171e-109
Identity = 180/180 (100.00%), Postives = 180/180 (100.00%), Query Frame = 2 
Query:  503 MVEETCVKDIWTXXXXXXXXXXXXXXXXXXXPFLSKIGCMLMNRSKADNDRISKLKELTNEKSKLNMTDNFAKYSKLERQIKKMESELQTNSQVRCKNSLTVRLVISMSLKILQVLTFMTIFYFWKNCVLNINSETLLIFIPTWMQHDFLTQPPMWCVLPVWTASCHVFVSTVLNYLLSE 1042
            MVEETCVKDIWTLLYVLLIIFIFKLIHLFCIPFLSKIGCMLMNRSKADNDRISKLKELTNEKSKLNMTDNFAKYSKLERQIKKMESELQTNSQVRCKNSLTVRLVISMSLKILQVLTFMTIFYFWKNCVLNINSETLLIFIPTWMQHDFLTQPPMWCVLPVWTASCHVFVSTVLNYLLSE
Sbjct:    1 MVEETCVKDIWTLLYVLLIIFIFKLIHLFCIPFLSKIGCMLMNRSKADNDRISKLKELTNEKSKLNMTDNFAKYSKLERQIKKMESELQTNSQVRCKNSLTVRLVISMSLKILQVLTFMTIFYFWKNCVLNINSETLLIFIPTWMQHDFLTQPPMWCVLPVWTASCHVFVSTVLNYLLSE 180
The following BLAST results are available for this feature:
BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
MED218.651e-1229.50mediator complex subunit 21 [Source:HGNC Symbol;Ac... [more]
MED218.845e-1228.00mediator complex subunit 21 [Source:HGNC Symbol;Ac... [more]
GET16.373e-827.47guided entry of tail-anchored proteins factor 1 [S... [more]
WRB-SH3BGR7.296e-827.47WRB-SH3BGR readthrough [Source:NCBI gene;Acc:10686... [more]
GET11.090e-730.99guided entry of tail-anchored proteins factor 1 [S... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mdt-212.175e-730.07Mediator of RNA polymerase II transcription subuni... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
MED216.980e-1735.97gene:FBgn0040020 transcript:FBtr0113739[more]
MED216.980e-1735.97gene:FBgn0040020 transcript:FBtr0113740[more]
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BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
med216.803e-1430.22mediator complex subunit 21 [Source:NCBI gene;Acc:... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
med212.009e-1230.94mediator complex subunit 21 [Source:NCBI gene;Acc:... [more]
med216.590e-728.35mediator complex subunit 21 [Source:NCBI gene;Acc:... [more]
nkain11.735e-628.57Sodium/potassium transporting ATPase interacting 1... [more]
med211.827e-628.35mediator complex subunit 21 [Source:NCBI gene;Acc:... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Med212.327e-1230.22mediator complex subunit 21 [Source:MGI Symbol;Acc... [more]
Med212.327e-1230.22mediator complex subunit 21 [Source:MGI Symbol;Acc... [more]
Med211.315e-1035.96mediator complex subunit 21 [Source:MGI Symbol;Acc... [more]
Wrb3.927e-727.47tryptophan rich basic protein [Source:MGI Symbol;A... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9W5P1|MED21_DROME6.997e-1635.97Mediator of RNA polymerase II transcription subuni... [more]
sp|Q16RX1|MED21_AEDAE4.000e-1533.81Mediator of RNA polymerase II transcription subuni... [more]
sp|Q7ZTI5|MED21_DANRE5.135e-1330.22Mediator of RNA polymerase II transcription subuni... [more]
sp|Q2TBU8|MED21_BOVIN5.009e-1230.22Mediator of RNA polymerase II transcription subuni... [more]
sp|Q5RE46|MED21_PONAB1.139e-1131.65Mediator of RNA polymerase II transcription subuni... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5K4FD932.066e-3343.51Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A4Z2DJF83.721e-3343.51Mediator of RNA polymerase II transcription subuni... [more]
Q5DD164.256e-3343.51Mediator of RNA polymerase II transcription subuni... [more]
A0A4S2L4H92.050e-3147.62Mediator of RNA polymerase II transcription subuni... [more]
A0A4Z2DKA29.376e-3143.62Mediator of RNA polymerase II transcription subuni... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
med211.157e-1330.22mediator complex subunit 21 [Source:NCBI gene;Acc:... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
get18.590e-731.34guided entry of tail-anchored proteins factor 1 [S... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO474129.404e-1934.56Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
med212.493e-1430.22mediator complex subunit 21 [Source:NCBI gene;Acc:... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 21 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000036235.12.171e-109100.00SMESG000036235.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011519 ID=SMED30011519|Name=Mediator of RNA polymerase II transcription subunit 21|organism=Schmidtea mediterranea sexual|type=transcript|length=1448bp
TTTTTTTTAAACACGTTCTAAAATTATTTTATGTTTTATTTTTAGGCAGA
TAGATTAAGTGAATTACAAGATATTATTAATCAACAAGCTCAAAATCTTT
GTGATTCAATTGGAGTTATTCAGCAGTTTGCCCAGCCTTCATTTTTTAGT
GAATTTGATTGGAAAGCTAGAAGTAGCAGAAAAGACAATCCTATAATACA
ATTAGTATTAGAAGATAAATCATCACCAGATTATTGTAAGCAATTTGCCA
AAGTTATTCGAGAAACTTTTACTCAAATTAATTATTTAATTGACGGCCTT
CCAGAAGAAGACTTATCACTTGAAATACATAACTCAGCACTTTCACTTTT
ACATAACGACTATATTGATAGCGGCAAAGAATTATTGAAAACAGTTGACT
ATTTAGAAAAAAAGTTGGAATTTGTTAGAGAAAAACTAATCGAAATATCT
GAAGTACAGTTGACAGCTGAAGCTCTCGAAGCCGAAATTTTAAAAAATTA
TTATGGTTGAAGAAACTTGTGTTAAAGATATTTGGACACTTCTATATGTT
CTTTTAATCATTTTTATTTTTAAACTTATACATTTGTTTTGTATTCCGTT
TCTTAGTAAAATTGGATGCATGCTTATGAATAGATCAAAAGCCGACAATG
ATCGAATCAGTAAACTTAAAGAATTGACAAACGAGAAGTCAAAATTAAAT
ATGACAGACAATTTCGCCAAATATTCAAAACTAGAAAGACAAATTAAGAA
AATGGAGAGTGAATTGCAAACTAATAGCCAAGTAAGGTGTAAAAACAGTC
TAACCGTGAGACTTGTTATATCAATGTCACTAAAAATTCTTCAAGTTTTA
ACATTTATGACAATTTTCTACTTTTGGAAAAATTGTGTGTTAAACATCAA
TTCTGAAACATTGTTAATATTTATTCCTACTTGGATGCAGCATGATTTTC
TTACTCAACCTCCAATGTGGTGCGTTTTACCTGTCTGGACTGCTAGCTGT
CATGTTTTTGTTTCAACTGTACTAAATTATTTGTTAAGTGAATAATTTTG
ATGTTAAATTTGAATTGATTTTAAATAGTTGTTAAATTGGTACTTTGATA
TTGATTTTATTATATTTATTTTTCAATTTTCAAGTAGTTCTACGCAGTTT
TTATCGTTTCGTTTTCATGGTAGTGTTGAAATATGCTCTCATCGTTCCAT
TTGATTGATTTTGATATGGTGATTTTAGTTCTTGCTTTATTCCCGATTTC
GATAAATTTGTATTTATCGTTGATGCTGACTAATTTACTTGATCGATCTA
TTATTACGACGACATTTTGTTACACTTTGACTCATGTATCCTATGTCAAT
TTGTTCTTGAAAGTTAACTTTCCTTTTTCGTTTCTGGTCAATTTTTCACA
GAAAATTGAAGACCAGAACAATGTTACTAATGTTTGTTCCGATTTGTC
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protein sequence of SMED30011519-orf-1

>SMED30011519-orf-1 ID=SMED30011519-orf-1|Name=SMED30011519-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=181bp
MVEETCVKDIWTLLYVLLIIFIFKLIHLFCIPFLSKIGCMLMNRSKADND
RISKLKELTNEKSKLNMTDNFAKYSKLERQIKKMESELQTNSQVRCKNSL
TVRLVISMSLKILQVLTFMTIFYFWKNCVLNINSETLLIFIPTWMQHDFL
TQPPMWCVLPVWTASCHVFVSTVLNYLLSE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0016592mediator complex
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000043dorsal ventral margin of the whole animal
PLANA:0000070intestinal phagocyte
Vocabulary: INTERPRO
TermDefinition
IPR028945WRB/Get1
IPR021384Mediator_Med21
Vocabulary: biological process
TermDefinition
GO:0071816tail-anchored membrane protein insertion into ER membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 67..94
NoneNo IPR availablePANTHERPTHR42650FAMILY NOT NAMEDcoord: 8..174
NoneNo IPR availableTMHMMTMhelixcoord: 10..32
NoneNo IPR availableTMHMMTMhelixcoord: 156..178
NoneNo IPR availableTMHMMTMhelixcoord: 102..124
IPR028945WRB/Get1 familyPFAMPF04420CHD5coord: 15..166
e-value: 1.2E-13
score: 51.3