SMED30011509

Overview
NameSMED30011509
Smed IDSMED30011509
Length (bp)2203
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30011509 (SMED30011509) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30011509 (SMED30011509) in cells (dots) of each of the 12 neoblast clusters.

 

back to top


 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30011509 (SMED30011509) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30011509 (SMED30011509) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top


 

Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30011509

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymal cellSMED30011509SMESG000014480.1 dd_Smed_v4_2606_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
pigment cellSMED30011509SMESG000014480.1 dd_Smed_v6_2606_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30011509SMESG000014480.1 dd_Smed_v6_2606_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30011509 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002736:43869..47013 +10869
Homology
BLAST of SMED30011509 vs. Planmine SMEST
Match: SMESG000014480.1 (SMESG000014480.1)

HSP 1 Score: 1292.33 bits (3343), Expect = 0.000e+0
Identity = 638/642 (99.38%), Postives = 638/642 (99.38%), Query Frame = 2
Query:  131 MKIAAVILIWIFLKFSKAGSLSISKISPFPHNIHKHFNITLSYSNESFGLGDTLVIRISDKFGNKFDEAVFDYGVKDEIGDTYSGEFMTVSKWEKNPVEKTLSASLFFNKRSNSQFIEILNIQLISSKFQNLSQENYGESILVYRSKIELLSPTKLKLTVSSVDGKVKEFEEGQIGVASCRLIGGTQNTLPVFINIKNFTIKRSSLILEKTERKFEFLISIADHMKEIICKSGNNIQKAKVKVFHPPTKVKLEPELNNKLVNNTKVSCGHLNGYPIPVAQWRLVHGPSNASTMYEITQGGQHLKVTVPRLDNSIWSFNCSARNSFSNSIKWFNKTVTFFIIENNKNKQINSENIKPIRKPENSTNHNELLLIKNTIELLSVNYLKLTKVTEKLITDNKNFSMALKETGKANLRLNREIEQLKKSCSVKTKKLRSNNKKEFFESAMKRKDDSIEIRNLKEGLEVLKKKINEMTELINRTPLDKAIHQPQQLKLIQTKPPDKTQGYPPFNISPVNEWKINQIQKKRKKAFPIFGNFFYADYNANKMKMLFFPNGYQISSATHLTVILQLIFAPDIDSYRPFKGDIIIGIYNSRNQYLNKIISRHLLLTRQVDRAIFNFKYDELELSGVLKSDEIIIKCFVIGKD 2056
            MKIAAVILIWIFLKFSKAGSLSISKISPFPHNIHKHFNITLSYSNESFGLGDTLVIRISDKFGNKFDEAVFDYGVKDEIGDTYSGEFMTVSKWEKNPVEKTLSASLFFNKRSNSQFIEILNIQLISSKFQNLSQENYGESILVYRSKIELLSPTKLKLTVSSVDGKVKEFEEGQIGVASCRLIGGTQNTLPVFINIKNFTIKRSSLILEKTERKFEFLISIADHMKEIICKSGNNIQKAKVKVFHPPTKVKLEPELNNK VNNTKVSCGHLNGYPIPVAQWRLVHGPSNASTMYEITQGGQHLKVTVPRLDNSIWSFNCSARNSFSNSIKWFNKTVTFFIIENNKNKQINSENIKPIRKPENSTNHNELLLIKNTIELLSVNYLKLTKVTEKLITDNKNFSMALKETGKANLRLNREIEQLKKSCSVKTKKLRSNNKKEFFESAMKRKDDSIEIRNLKEGLEVLKKKINEMTELINRTPLDKAIHQPQQLKLIQTKPPDKTQGYPPFNISPVNEWKINQIQKKRKKAFPIFGNFFYADYNANKMKMLFFPNGYQ SSATHLTVILQLIFAPDIDSYRPFKGDIIIGIYNSRNQYLNKIISRHLLLT QVDRAIFNFKYDELELSGVLKSDEIIIKCFV GKD
Sbjct:    1 MKIAAVILIWIFLKFSKAGSLSISKISPFPHNIHKHFNITLSYSNESFGLGDTLVIRISDKFGNKFDEAVFDYGVKDEIGDTYSGEFMTVSKWEKNPVEKTLSASLFFNKRSNSQFIEILNIQLISSKFQNLSQENYGESILVYRSKIELLSPTKLKLTVSSVDGKVKEFEEGQIGVASCRLIGGTQNTLPVFINIKNFTIKRSSLILEKTERKFEFLISIADHMKEIICKSGNNIQKAKVKVFHPPTKVKLEPELNNKFVNNTKVSCGHLNGYPIPVAQWRLVHGPSNASTMYEITQGGQHLKVTVPRLDNSIWSFNCSARNSFSNSIKWFNKTVTFFIIENNKNKQINSENIKPIRKPENSTNHNELLLIKNTIELLSVNYLKLTKVTEKLITDNKNFSMALKETGKANLRLNREIEQLKKSCSVKTKKLRSNNKKEFFESAMKRKDDSIEIRNLKEGLEVLKKKINEMTELINRTPLDKAIHQPQQLKLIQTKPPDKTQGYPPFNISPVNEWKINQIQKKRKKAFPIFGNFFYADYNANKMKMLFFPNGYQTSSATHLTVILQLIFAPDIDSYRPFKGDIIIGIYNSRNQYLNKIISRHLLLTHQVDRAIFNFKYDELELSGVLKSDEIIIKCFVSGKD 642          
BLAST of SMED30011509 vs. Planmine SMEST
Match: SMESG000014710.1 (SMESG000014710.1)

HSP 1 Score: 179.104 bits (453), Expect = 3.278e-48
Identity = 158/589 (26.83%), Postives = 265/589 (44.99%), Query Frame = 2
Query:  290 LVIRISDKFGNKFDEAVFDYGVKDEIGDTYSGEFMTVSKWEKNPVEKTLSASLFFNKRSNSQFIEILNIQLISSKFQNLSQENYGESILVYRSKIELLSPTKLKLTVSSVDGKVKEFEEGQIGVASCRLIGGTQNTLPVFINIKNFTIKRSSLILEKTERKFEFLISIADHMKEIICKSGNNIQKAKVKVFHPPTKVKLEPELNNKLVNNTKVSCGHLNGYPIPVAQWRLVHGPSNASTMYEITQGGQHLKVTVPRLDNSIWSFNCSARNSFSNSIKWFNKTVTFFIIENNKNKQ---INSENIKPIRKPENSTNHNELLLIKNTIELLSVNYLKLTKVTEKLITDNKNFSMALKETGKANLRLNREIEQLKKSCSVKTKKLRSNNKKEFFESAMKRKDDSIEIRNLKEGLEVLKKKINEMTELINRTPLDK-AIHQPQQLKLIQTKPPDKTQGYPPFNISPVNEWKINQIQKKRKKAFPIFGNFFYADYNANKMKMLFFPNG--YQISSATHLTVILQLIFAPDIDSYRPFKGDIIIGIYNSRNQYLNKIISRHLLLTRQVDRAIFNFKYDELELSGVLKSDEIIIKC 2038
            +VI+ +D  G   + A+FD+G+K+ I   +  ++M +  W K     T+   + F + +N++ +EI  I   S+K +     +   + L+YR+ I LL P +L+L ++S        E       SC+ + G     P  +   +  I+      +    K     S+ DH + + CK+G+ ++  ++ ++H PTK+KL+PE+   L NNT + C   NGYP P   W  +HGPS+AS  Y+I    Q L +       S W F CSA NSF    K FNKTV+F II   +NK     N+  IK      +  N+           L  +  +KL      +   N   S+           LN EI QL ++     ++LR   ++   +  M +K  +IEI       E LKK++N +    N   +DK  ++Q Q+   + T P         F      +W+IN + + R+K      +F    +   K++   FP    ++      + + +++    D +S    KG + + IYN+ +              R+     F F Y EL ++ VL+SD+II+KC
Sbjct:    1 MVIQTTDLNGFDKEVAIFDFGLKEVISQEFQSDYMRIPTWGKFEEGATIYTMINFTQYANNRILEIYYINPNSTKERRNMLNHLKPTQLMYRNNINLLVPPQLELKMNSQVRNYITVEPNSSLTVSCQALDGQPKVRPEIVK-SDMKIEFDIFSHDSNTHKITIRTSMLDHNRTVTCKAGSVMKSVRIFIYHAPTKIKLQPEVKKVLGNNTGIICLATNGYPTPRITWYFIHGPSDASLYYQIAPYTQRLYIKENVPIGSSWKFICSATNSFFERSKTFNKTVSFTIIHQQRNKTQTYYNTSKIKTSIWDPDQMNYT----------LKQIEEIKLEIFVRDITITNLKDSIGT---------LNSEIFQLNQTI----QQLRETQRETSSDIKMFKKIFNIEI-------ESLKKELNNL----NGNLIDKNKVNQTQRNSAMTTTPSASLVPIISF------DWRINGVSRLRRKE----KSFRSVKFVNPKIR---FPVTLYFKFRRMESMFISVRMSHINDAESSLVIKGYVTLSIYNAEDVSKQMSTRNFFERNRRSTNVDFVFNYFELHMNQVLQSDDIIVKC 541          
BLAST of SMED30011509 vs. Planmine SMEST
Match: SMESG000069999.1 (SMESG000069999.1)

HSP 1 Score: 169.859 bits (429), Expect = 1.060e-44
Identity = 98/302 (32.45%), Postives = 158/302 (52.32%), Query Frame = 2
Query:  278 LGDTLVIRISDKFGNKFDEAVFDYGVKDEIGDTYSGEFMTVSKWEKNPVEKTLSASLFFNKRSNSQFIEILNIQLISSKFQNLSQENYGESILVYRSKIELLSPTKLKLTVSSVDGKVKEFEEGQIGVASCRLIGGTQNTLPVFINIKNFTIKRSSLILEKTERKFEFLISIADHMKEIICKSGNNIQKAKVKVFHPPTKVKLEPELNNKLV-NNTKVSCGHLNGYPIPVAQWRLVHGPSNASTMYEITQGGQHLKVTVPRLDNSIWSFNCSARNSFSNSIKWFNKTVTFFIIENNKNKQIN 1180
            +GDT++I+I+D  G      +F YG+KD I +   G++M + +WE N  + +++  + F+K SN    EI  I   S +      +N   +  +Y+  IEL  P  L  TV+SV G   ++E     +A CR   G  N +P F N + F+   S+  LE+ ER     +   +HM  + C++G+ ++   +KVFH PT++ ++P+L  KLV +NT   C   +G+P P  +W LV GP+N S  YE+     +L +     ++S W F C   +   NS +   K ++FF  ENN   Q N
Sbjct:   58 VGDTMLIQITDNIGLIKQREIFYYGIKDTIENEIKGDYMNIKRWEVNYNDSSINLFIIFHKNSNFHLFEIYFIDAPSKEILKKKIKNLRLAKRIYKRTIELFHPPTLSFTVNSVLGAHSQWEINDTIIAICRAFDGIPNEIPKFENYQGFSNISSTFSLERNERMINMTVGTENHMNPLQCRAGSEVKVHTIKVFHHPTELFIKPKL--KLVYSNTTFQCYAKDGFPTPTMEWFLVEGPANHSNYYELHGNRSYLFIKDNCPNHSKWKFKCKLTHMIRNSSRTLVKMISFF-FENNTIGQCN 356          
BLAST of SMED30011509 vs. Planmine SMEST
Match: SMESG000032651.1 (SMESG000032651.1)

HSP 1 Score: 165.236 bits (417), Expect = 1.908e-43
Identity = 98/328 (29.88%), Postives = 164/328 (50.00%), Query Frame = 2
Query:  191 LSISKISPFPHNIHKHFNITLS--------YSNESFGLGDTLVIRISDKFGNKFDEAVFDYGVKDEIGDTYSGEFMTVSKWEKNPVEKTLSASLFFNKRSNSQFIEILNIQLISSKFQNLSQENYGESILVYRSKIELLSPTKLKLTVSSVDGKVKEFEEGQIGVASCRLIGGTQNTLPVFINIKNFTIKRSSLILEKTERKFEFLISIADHMKEIICKSGNNIQKAKVKVFHPPTKVKLEPELNNKLVNNTKVSCGHLNGYPIPVAQWRLVHGPSNASTMYEITQGGQHLKVTVPRLDNSIWSFNCSARNSFSNSIKWFNKTVTFFI 1150
            L ++ I  FP     +F I +         Y N      D L+I+ISD   +K  +A+F+Y  KD I     G+FM +  W K   +  + A++ F++ +N + +EI  I   ++  +    +   ++  +Y+SKI LL   KL+L ++S+DG+  E    + G ASCR   G     P +    +  ++      E+ ER  E   SI DHMKE+ C +G      K+ V H PT++ L+PEL + + N T   C  ++G+P+P  +W  + GP +    + I++ G+ L +       ++W FNC+A N    +   FN+TV+F I
Sbjct:   28 LRVNTIPEFPFPAGSNFTIEIVEKENGKQIYGNSH----DILIIQISD--SSKKQQAIFEYIYKDMIDMEIKGDFMRIPTWTK--FKSQIIATMLFDRSANDKTLEIFQITASNNNEKRNQMKTLNKNSRIYQSKISLLIDPKLELLITSMDGRKFELMLDEWGKASCRAFDGVPMEKPYWDKNFDLKMRNPKYSPERNERTIEIYASILDHMKELSCTAGRTRAAVKINVLHHPTQILLDPELGSFVENGTTTYCSSIDGFPVPEVRWHFISGPDDPGRYFNISKDGRKLLLKSETPIGTVWKFNCTASNILPRTKGSFNRTVSFEI 347          
BLAST of SMED30011509 vs. Planmine SMEST
Match: SMESG000070014.1 (SMESG000070014.1)

HSP 1 Score: 158.303 bits (399), Expect = 8.094e-41
Identity = 138/481 (28.69%), Postives = 214/481 (44.49%), Query Frame = 2
Query:  143 AVILIWIFLKFSKAGSLSISKISPFPHNIHKHFNITL----SYSNESFGLGDTLVIRISDKFGNKFDEAVFDYGVKDEIGDTYSGEFMTVSKWEKNPVEKTLSASLFFNKRSNSQFIEILNIQLISSKFQNLSQENYGESILVYRSKIELLSPTKLKLTVSSVDGKVKEFEEGQIGVASCRLIGGTQNTLPVFINIKNFTIKRSSLILEKTERKFEFLISIADHMKEIICKSGNNIQKAKVKVFHPPTKVKLEPELNNKLVNNTKVSCGHLNGYPIPVAQWRLVHGPSNASTMYEITQGGQHLKVTVPRLDNSIWSFNCSARNSFSNSIKWFNKTVTFFIIENNKNKQINSENIKPIRKPENSTNHNELLLIKNTIELLSVNYLKLTKVTEKLITDNKNFSMALKETGKANLRLNREIEQLKKSCSVKTKKLRSNNKKEFFESAMKRKDDSIEIRNLKEGLEVLKKKINEMTELINRTPLD 1573
            AV+++   + F    SL+   +S FP  +++ F   +       N +  L DT+VI+I D  G   ++A+F YG  +++   + G FM + KWE N     +  S+ F  +SN   IEI  +   S      S ++   +  ++R+ I LLSP+K+ L  S        +        +CR  GG    +P+F N K+F I  SS   EK ER  +FL+   DH+K I C +GN  +   +KV H P +V ++    NK   N ++ C  + G+P P  +W L  GP +AS  Y IT  G  L +      NS W F C       + I    +T +                   + KP+ S N +        +E LS N+ K  +  E + T N        E    NLR+  EI+Q     S  T  L    K+E       +K D   I +LK  L  +++K+ E  + IN+T +D
Sbjct:    8 AVVILCGLVGFH-VESLTAKALSKFPFLMNETFQFQILPKTKKGNLTVSLLDTMVIQIVDMAGLVKEKAIFHYGFHEKLDQEFHGSFMKIVKWELNSNASAIDISIIFQPKSNFYIIEIYYVCPQSKSMMKKSLKDLNLAYRIFRNSINLLSPSKVDLRASPNQQNDFIWNLNDTVTVTCRAYGGMPKRIPLFENFKSFAIINSSFFPEKNERMIKFLVRREDHLKTIKCGAGNISKSQIIKVKHRPLQVYIK---QNK---NKEIDCSVVGGFPTPSIKWALTSGPQDASKFYNITSSGTRLFINEKSPLNSKWEFECQISQRLGSFITHTKRTFS-----------------TVVEKPKISCNTDCANKKITNLEHLSENFQKSLQNIENIQTKN--------EKNTDNLRM--EIKQELFQISSLTLNLVKELKQENVNLKQIQKSD---INSLKGDLAEIRQKMKE--KFINKTEID 449          
The following BLAST results are available for this feature:
BLAST of SMED30011509 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011509 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011509 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011509 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011509 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011509 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011509 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011509 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011509 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011509 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011509 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011509 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011509 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30011509 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000014480.10.000e+099.38SMESG000014480.1[more]
SMESG000014710.13.278e-4826.83SMESG000014710.1[more]
SMESG000069999.11.060e-4432.45SMESG000069999.1[more]
SMESG000032651.11.908e-4329.88SMESG000032651.1[more]
SMESG000070014.18.094e-4128.69SMESG000070014.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011509 ID=SMED30011509|Name=SMED30011509|organism=Schmidtea mediterranea sexual|type=transcript|length=2203bp
CGCTAGCATCCAAATCTAAGTCCAATCTGTGTCAGTGAACTTTGACTAGT
CTTATTCGTCTCCAGGATTCGACGTATCCGTTTTAGAATTCGTTTCAAAA
TGACGTATTTTAAATATTAGACTTCTTAGAATGAAAATCGCTGCTGTTAT
ATTAATTTGGATTTTTCTCAAGTTTTCCAAGGCTGGGTCATTGTCAATTT
CAAAGATTTCCCCATTCCCCCATAATATCCATAAACATTTCAACATCACT
CTAAGCTATTCTAATGAAAGCTTTGGGCTGGGTGACACGTTGGTCATTAG
AATATCAGACAAATTCGGGAATAAATTTGACGAAGCGGTCTTCGATTATG
GGGTTAAAGATGAAATTGGTGATACCTACTCAGGAGAATTTATGACTGTT
TCCAAATGGGAAAAGAACCCCGTCGAGAAAACCCTTTCGGCATCTTTATT
TTTCAATAAAAGATCTAACAGCCAATTTATTGAAATTCTTAATATTCAGC
TGATTTCAAGTAAGTTTCAAAACCTCTCTCAGGAAAACTACGGAGAGTCT
ATATTAGTTTATCGCTCAAAAATTGAATTATTATCCCCCACAAAACTCAA
ATTGACAGTAAGTAGTGTAGACGGAAAGGTAAAAGAATTTGAGGAAGGTC
AAATCGGAGTTGCAAGCTGTAGACTCATTGGTGGCACTCAAAATACACTT
CCGGTTTTTATAAATATCAAAAACTTTACAATCAAACGTAGCTCACTGAT
ACTTGAAAAAACAGAAAGAAAGTTCGAATTCCTCATCTCAATTGCTGACC
ATATGAAGGAAATAATATGTAAGTCAGGAAACAATATTCAAAAAGCAAAG
GTAAAAGTATTTCATCCGCCAACTAAAGTTAAACTTGAACCGGAATTAAA
TAATAAACTTGTCAACAACACCAAAGTAAGTTGTGGTCATTTAAATGGCT
ATCCGATTCCTGTTGCGCAATGGAGATTAGTACATGGACCCTCTAATGCT
AGCACAATGTATGAAATAACTCAAGGAGGACAACATTTAAAGGTTACAGT
TCCAAGATTAGATAATTCAATTTGGAGTTTTAATTGTTCGGCAAGAAACT
CTTTTTCAAACTCCATAAAATGGTTTAATAAAACTGTTACATTCTTCATA
ATTGAAAATAATAAAAACAAACAAATAAATTCGGAAAATATTAAACCAAT
CAGAAAACCCGAAAATTCAACCAATCACAACGAACTATTGTTAATTAAAA
ATACTATTGAATTATTGTCTGTAAACTACTTAAAACTGACAAAGGTTACC
GAAAAGCTAATAACGGACAATAAAAACTTTTCCATGGCCCTGAAAGAAAC
AGGAAAAGCGAATTTGCGACTAAATCGGGAGATTGAGCAGTTGAAAAAAA
GTTGCTCTGTTAAAACAAAAAAGTTAAGATCCAACAATAAAAAGGAATTT
TTCGAATCAGCAATGAAACGCAAAGACGACTCAATTGAGATCAGAAATCT
AAAAGAGGGCTTGGAAGTATTGAAGAAAAAGATCAATGAAATGACAGAAC
TCATTAATAGAACCCCTCTTGACAAGGCGATTCATCAACCCCAGCAATTG
AAACTAATTCAAACAAAACCTCCCGACAAGACTCAAGGCTATCCCCCATT
TAATATCTCTCCAGTAAACGAGTGGAAAATCAACCAAATCCAAAAGAAGC
GTAAAAAAGCATTTCCGATTTTCGGGAATTTTTTTTATGCCGATTACAAC
GCAAACAAAATGAAAATGCTGTTCTTTCCGAATGGCTACCAAATATCTTC
AGCTACTCATCTGACAGTGATTTTGCAGTTGATCTTTGCACCCGACATTG
ACTCCTACAGACCCTTCAAAGGAGACATCATCATCGGCATATACAACAGT
CGCAATCAGTATCTCAATAAGATTATCTCGCGACATTTGCTTTTGACTCG
CCAAGTCGATCGCGCAATTTTCAATTTCAAATACGACGAATTGGAGCTTT
CTGGAGTGTTGAAGAGCGACGAAATCATCATCAAATGTTTTGTTATTGGG
AAAGATTAAAAAAAAACAGTTTTGATTCCGTAAATCAGCGACAGGCAAAT
ATTTATGTAAATTTTTGAAAATGATGTTAGTAAATGTTTTAGATTTTTAA
AGATAACTTGACAGAAATGAAGATTTATTTGTAATTAAATTCAAATAAAT
GCC
back to top

protein sequence of SMED30011509-orf-1

>SMED30011509-orf-1 ID=SMED30011509-orf-1|Name=SMED30011509-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=643bp
MKIAAVILIWIFLKFSKAGSLSISKISPFPHNIHKHFNITLSYSNESFGL
GDTLVIRISDKFGNKFDEAVFDYGVKDEIGDTYSGEFMTVSKWEKNPVEK
TLSASLFFNKRSNSQFIEILNIQLISSKFQNLSQENYGESILVYRSKIEL
LSPTKLKLTVSSVDGKVKEFEEGQIGVASCRLIGGTQNTLPVFINIKNFT
IKRSSLILEKTERKFEFLISIADHMKEIICKSGNNIQKAKVKVFHPPTKV
KLEPELNNKLVNNTKVSCGHLNGYPIPVAQWRLVHGPSNASTMYEITQGG
QHLKVTVPRLDNSIWSFNCSARNSFSNSIKWFNKTVTFFIIENNKNKQIN
SENIKPIRKPENSTNHNELLLIKNTIELLSVNYLKLTKVTEKLITDNKNF
SMALKETGKANLRLNREIEQLKKSCSVKTKKLRSNNKKEFFESAMKRKDD
SIEIRNLKEGLEVLKKKINEMTELINRTPLDKAIHQPQQLKLIQTKPPDK
TQGYPPFNISPVNEWKINQIQKKRKKAFPIFGNFFYADYNANKMKMLFFP
NGYQISSATHLTVILQLIFAPDIDSYRPFKGDIIIGIYNSRNQYLNKIIS
RHLLLTRQVDRAIFNFKYDELELSGVLKSDEIIIKCFVIGKD*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002075pigment cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR008974TRAF-like
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 404..424
NoneNo IPR availableCOILSCoilCoilcoord: 454..474
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..18
score: 0.798
IPR008974TRAF-likeGENE3DG3DSA:2.60.210.10coord: 512..639
e-value: 8.9E-7
score: 31.1
IPR008974TRAF-likeSUPERFAMILYSSF49599TRAF domain-likecoord: 511..638