EF-hand domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30011490 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10
Homology
BLAST of EF-hand domain-containing protein vs. UniProt/SwissProt
Match: sp|P14202|TEGU_SCHMA (Tegument antigen OS=Schistosoma mansoni OX=6183 GN=A12 PE=2 SV=1) HSP 1 Score: 65.4698 bits (158), Expect = 5.616e-12 Identity = 49/188 (26.06%), Postives = 104/188 (55.32%), Query Frame = 2 Query: 38 AQIKMATMDDAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFCDVXXXXXXXXXXXXXXXXXXXXXX--XXPKSDQMLDDIIEKTKELR-----KEHPDQN-------KIVASELKKYIEERYQRAWVILIIRGQYWAHYSYLPEDNFVFKLNNDTHVIFRSMS 559 + K++ M++ F+ AF ID + + I ++L+++ K D K +D W+ FD ++ I++EEFC GL++ E+ ++++ ++E++ + P + +++ + KTK+ KE+ D + VA+++K ++ Y R W ++++ G YW ++S+ P + FK NN + +R+ S Sbjct: 3 TETKLSQMEE-FIRAFLEIDADSNEMIDKQELIKYCQKYRLDMKLIDPWIARFDTDKDNKISIEEFCRGFGLKVSEIRREKDELKKERDGKFPKLPPNIEIIAATMSKTKQYEICCQFKEYVDNTSRTGNDMREVANKMKSLLDNTYGRVWQVVLLTGSYWMNFSHEPFLSIQFKYNNYVCLAWRTPS 189
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A0X3NX37 (Antigen Sm21.7 OS=Schistocephalus solidus OX=70667 GN=SM21 PE=4 SV=1) HSP 1 Score: 100.908 bits (250), Expect = 5.907e-23 Identity = 64/172 (37.21%), Postives = 105/172 (61.05%), Query Frame = 2 Query: 65 DAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFCDVXXXXXXXXXXXXXXXXXXXXXXXXPKSD-QMLDD--------IIEKTKELRKEHPDQNKIVASELKKYIEERYQRAWVILIIRGQYWAHYSYLPEDNFVFKLNNDTHVIFRS 553 DA + AFF+ID+ +T KIT L +++ N+ DE F+++W LFD +GVITL+ FCDVLGLEL E++E R+K+ + P Q+ +D I E KE +++ D + + LK ++E+Y R W + ++RGQY+A+YSY +++ FK + +V+F++ Sbjct: 2 DALMEAFFTIDKDHTDKITMDALEQYRLDNDLDESFINQWKKLFDPGNSGVITLQTFCDVLGLELAEVQEDRDKFVAAATPSTIPAGILQIAEDMDLQRRLEIFELVKEAEEKNGDNERDIVKWLKLILDEKYGRVWHVFVVRGQYYAYYSYEAGNSYCFKKDKRIYVVFKT 173
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A267F2V4 (Dynein light chain (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034129g2 PE=3 SV=1) HSP 1 Score: 98.9821 bits (245), Expect = 2.763e-22 Identity = 57/173 (32.95%), Postives = 95/173 (54.91%), Query Frame = 2 Query: 53 ATMDDAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFC---DVXXXXXXXXXXXXXXXXXXXXXXXXPKSDQMLD---DIIEKTKELRKEHPDQNKIVASELKKYIEERYQRAWVILIIRGQYWAHYSYLPEDNFVFKLNNDTHVIFRS 553 TM DAF+ A+F+ID + +IT+ QL + +NNYDE FV KWL LFD + +G ITL+E+C + ++K + + SD L+ IIE ++ ++ + K +A +K+ + + +R W ++II GQ+W YS+ P +F+F+L I+R+ Sbjct: 3 VTMTDAFIDAYFAIDTEGKGEITSAQLTRYMRQNNYDEAFVRKWLKLFDSDNSGTITLDEYCQTLGLEAKKVKADYIAKMRSGSLPSDITVISSDMTLELQLRIIEIVRDGQRLNS-MEKDMAKYIKQQCDAKMERLWHVVIINGQFWCWYSHEPGYSFIFRLGKYIFCIWRT 174
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A2H1CKG3 (EF hand OS=Fasciola hepatica OX=6192 GN=D915_04679 PE=4 SV=1) HSP 1 Score: 98.2117 bits (243), Expect = 6.003e-22 Identity = 54/172 (31.40%), Postives = 97/172 (56.40%), Query Frame = 2 Query: 65 DAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFCD--------VXXXXXXXXXXXXXXXXXXXXXXXXPKSDQMLDDIIEKTKELRKE--HPDQNKIVASELKKYIEERYQRAWVILIIRGQYWAHYSYLPEDNFVFKLNNDTHVIFR 550 D F+++F ++D + +IT +L ++ NN D V KW+ LFD ++TGVITLE FCD V + + +R ++ P DQ+ I+++TK+L K +PD ++S+LK++ ++ + AW + I+ G YW +S+LPE++F F +N ++ ++ Sbjct: 2 DTFINSFLAVDADGSGQITEDELRQYVTDNNMDSMMVTKWMQLFDPDKTGVITLENFCDKLGLKPEEVERQRAETTQAERIPSDIMVIYDKLPIKDQV--QIVKETKKLAKNVNNPDDYIQLSSQLKQFADKEFGSAWQVAIVEGSYWITFSHLPENSFQFVYDNHAYLFWK 171
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A4E0RHQ2 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_003074 PE=4 SV=1) HSP 1 Score: 98.2117 bits (243), Expect = 7.857e-22 Identity = 54/172 (31.40%), Postives = 97/172 (56.40%), Query Frame = 2 Query: 65 DAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFCD--------VXXXXXXXXXXXXXXXXXXXXXXXXPKSDQMLDDIIEKTKELRKE--HPDQNKIVASELKKYIEERYQRAWVILIIRGQYWAHYSYLPEDNFVFKLNNDTHVIFR 550 D F+++F ++D + +IT +L ++ NN D V KW+ LFD ++TGVITLE FCD V + + +R ++ P DQ+ I+++TK+L K +PD ++S+LK++ ++ + AW + I+ G YW +S+LPE++F F +N ++ ++ Sbjct: 18 DTFINSFLAVDADGSGQITEDELRQYVTDNNMDSMMVTKWMQLFDPDKTGVITLENFCDKLGLKPEEVERQRAETTQAERIPSDIMVIYDKLPIKDQV--QIVKETKKLAKNVNNPDDYIQLSSQLKQFADKEFGSAWQVAIVEGSYWITFSHLPENSFQFVYDNHAYLFWK 187
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A1I8H6V5 (Dynein light chain OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034129g1 PE=3 SV=1) HSP 1 Score: 96.6709 bits (239), Expect = 1.991e-21 Identity = 56/171 (32.75%), Postives = 94/171 (54.97%), Query Frame = 2 Query: 59 MDDAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFC---DVXXXXXXXXXXXXXXXXXXXXXXXXPKSDQMLD---DIIEKTKELRKEHPDQNKIVASELKKYIEERYQRAWVILIIRGQYWAHYSYLPEDNFVFKLNNDTHVIFRS 553 M DAF+ A+F+ID + +IT+ QL + +NNYDE FV KWL LFD + +G ITL+E+C + ++K + + SD L+ IIE ++ ++ + K +A +K+ + + +R W ++II GQ+W YS+ P +F+F+L I+R+ Sbjct: 1 MTDAFIDAYFAIDTEGKGEITSAQLTRYMRQNNYDEAFVRKWLKLFDSDNSGTITLDEYCQTLGLEAKKVKADYIAKMRSGSLPSDITVISSDMTLELQLRIIEIVRDGQRLNS-MEKDMAKYIKQQCDAKMERLWHVVIINGQFWCWYSHEPGYSFIFRLGKYIFCIWRT 170
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000026288.1 (SMESG000026288.1) HSP 1 Score: 298.13 bits (762), Expect = 5.138e-104 Identity = 171/171 (100.00%), Postives = 171/171 (100.00%), Query Frame = 2 Query: 50 MATMDDAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFCDVXXXXXXXXXXXXXXXXXXXXXXXXPKSDQMLDDIIEKTKELRKEHPDQNKIVASELKKYIEERYQRAWVILIIRGQYWAHYSYLPEDNFVFKLNNDTHVIFRSMSD 562 MATMDDAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFCDVLGLELKEMEEKREKWQEEKEKNREPKSDQMLDDIIEKTKELRKEHPDQNKIVASELKKYIEERYQRAWVILIIRGQYWAHYSYLPEDNFVFKLNNDTHVIFRSMSD Sbjct: 1 MATMDDAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFCDVLGLELKEMEEKREKWQEEKEKNREPKSDQMLDDIIEKTKELRKEHPDQNKIVASELKKYIEERYQRAWVILIIRGQYWAHYSYLPEDNFVFKLNNDTHVIFRSMSD 171
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000026288.1 (SMESG000026288.1) HSP 1 Score: 290.812 bits (743), Expect = 3.040e-101 Identity = 168/168 (100.00%), Postives = 168/168 (100.00%), Query Frame = 2 Query: 59 MDDAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFCDVXXXXXXXXXXXXXXXXXXXXXXXXPKSDQMLDDIIEKTKELRKEHPDQNKIVASELKKYIEERYQRAWVILIIRGQYWAHYSYLPEDNFVFKLNNDTHVIFRSMSD 562 MDDAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFCDVLGLELKEMEEKREKWQEEKEKNREPKSDQMLDDIIEKTKELRKEHPDQNKIVASELKKYIEERYQRAWVILIIRGQYWAHYSYLPEDNFVFKLNNDTHVIFRSMSD Sbjct: 1 MDDAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFCDVLGLELKEMEEKREKWQEEKEKNREPKSDQMLDDIIEKTKELRKEHPDQNKIVASELKKYIEERYQRAWVILIIRGQYWAHYSYLPEDNFVFKLNNDTHVIFRSMSD 168
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000006950.1 (SMESG000006950.1) HSP 1 Score: 98.9821 bits (245), Expect = 9.294e-26 Identity = 57/176 (32.39%), Postives = 92/176 (52.27%), Query Frame = 2 Query: 59 MDDAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFC-----------DVXXXXXXXXXXXXXXXXXXXXXXXXPKSDQMLDDIIEKTKELRKEHPDQNKIVASELKKYIEERYQRAWVILIIRGQYWAHYSYLPEDNFVFKLNNDTHVIFRS 553 MDDAF+ A+ ID+ + ITA +L + +NNY E FV KWL LFDR+ TG ITLEEFC D + + N ++ ++ +I K +EL +P+ K ++ +K+ ++ + W +I +GQYWA+YS PE + K+ N ++++R+ Sbjct: 1 MDDAFIKAYCQIDKDGSGTITADELRAYMTENNYQEDFVTKWLRLFDRDNTGRITLEEFCETLGLEVSDFIDKRTALKESADFSLYNGVNILSSNMVQPNEGIVIQVIRKAREL---YPNSFKEQSTYIKQNLDLVLETTWHCIITKGQYWAYYSCEPECSLAVKIENIVYLLWRT 173
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000045029.1 (SMESG000045029.1) HSP 1 Score: 78.9518 bits (193), Expect = 2.729e-18 Identity = 48/171 (28.07%), Postives = 89/171 (52.05%), Query Frame = 2 Query: 65 DAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFCDVXXXXXXXXXXXXXXXXXXXXXXXXPKSDQMLDDIIEKTKELRKEHPDQNKIVASELKKYIEE--------RYQRAWVILIIRGQYWAHYSYLPEDNFVFKLNNDTHVIFRS 553 D+F+ AFF+ID ++ +I+ +L ++ NKN+Y +F+ KWL +FD + +G ITLEE+C V +E KR Q P++ ++L +++ + N I + + K I + Y W +I+GQ+W ++S+L + + ++ +IFR+ Sbjct: 2 DSFIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKV-----LGIEYKRASLQRRMSMYSIPENVKILQSTMDEGQRNNCIKIYLNAISSGDSPKGILKDVHENLILYYGTGWQCFLIKGQHWTYFSHLEGCSLIIHIDGFIFIIFRT 167
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000026622.1 (SMESG000026622.1) HSP 1 Score: 72.4034 bits (176), Expect = 1.092e-15 Identity = 58/180 (32.22%), Postives = 96/180 (53.33%), Query Frame = 2 Query: 65 DAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDRERTGVITLEEFCDVXXXXXXXXXXXXXXXXXXXXXXXXPKSDQMLDD--IIEK--TKELRKEHPD---------QNKIVASELKKYIEER----YQRAWVILIIRGQYWAHYSYLPEDNFVFKLNNDTHVIFRS 553 D F+ AFF+ID +++IT +L ++ NN + FV KW+N+FD GVITL+E+C V L L E + + Q E + PK+ + DD II+ T E+ E D QN A ++ K+I+E Y +W + + GQ+W+++S P +F +K ++I+++ Sbjct: 2 DNFLTAFFNIDSDQSEEITIAELEKYMRLNNLKDAFVKKWINIFDSNDDGVITLDEYCSV--LGLVTNEARIMREQFNIEHSAIPKT--LPDDVKIIQSSVTSEMEIEMVDIEINGWSKCQN---APQVSKFIKEELDKAYGPSWHCVTVLGQFWSYFSSAPATSFQYKRGKYIYLIWKT 174 The following BLAST results are available for this feature:
BLAST of EF-hand domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 1
BLAST of EF-hand domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of EF-hand domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30011490 ID=SMED30011490|Name=EF-hand domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=670bpback to top protein sequence of SMED30011490-orf-1 >SMED30011490-orf-1 ID=SMED30011490-orf-1|Name=SMED30011490-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=172bp MATMDDAFVHAFFSIDRQNTKKITAKQLLEFQNKNNYDEKFVDKWLNLFDback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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